CN105552466B - A kind of p-nitrophenol concentration detection method based on microbiological fuel cell - Google Patents
A kind of p-nitrophenol concentration detection method based on microbiological fuel cell Download PDFInfo
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- CN105552466B CN105552466B CN201510761833.4A CN201510761833A CN105552466B CN 105552466 B CN105552466 B CN 105552466B CN 201510761833 A CN201510761833 A CN 201510761833A CN 105552466 B CN105552466 B CN 105552466B
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M10/00—Secondary cells; Manufacture thereof
- H01M10/42—Methods or arrangements for servicing or maintenance of secondary cells or secondary half-cells
- H01M10/48—Accumulators combined with arrangements for measuring, testing or indicating the condition of cells, e.g. the level or density of the electrolyte
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/10—Energy storage using batteries
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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Abstract
The invention discloses a kind of p-nitrophenol concentration detection system and method based on microbiological fuel cell, belong to microbial technology field, magnetic stirring apparatus is equipped with anode chamber, cathode chamber, anode chamber, the centre of cathode chamber are provided with PEM, the outside of cathode chamber and anode chamber is provided with negative electrode pumping equipment, anode electrode is provided with anode chamber, the negative pole of anode electrode and amplification chip connects, cathode electrode is provided with cathode chamber, the positive pole of cathode electrode and amplification chip connects, amplification detection voltage.It can realize on-line monitoring and two functions of in-situ immobilization simultaneously, there is the characteristics of simple to operate, equipment cost is low and stable compared with traditional detection mode and pollutant process means, this greatly optimizes data final process and the form showed, reduce the processing cost of pollutant, more more equipment remote monitoring simultaneously can be realized, data can be collected, convenient big data analysis later.
Description
Technical field：
The present invention relates to microbial technology field, and in particular to a kind of p-nitrophenol based on microbiological fuel cell is dense
Spend detection method.
Background technology：
P-nitrophenol (p-nitrophenol, PNP) is as main in the industry industrial wastewater such as chemical industry, agricultural chemicals, dyestuff
One of pollutant, have the characteristics that toxicity is big, be difficult to biodegradation.In order to ensure health, PNP concentration should be less than 70 in water
μ g/L, American States provide that PNP concentration must not be higher than 20 μ g/L in drinking water.Therefore, accurately PNP contains measurer in detection water outlet
It is significant.At present, it is exactly ultraviolet-visible spectrophotometry that p-nitrophenol, which detects most common method,.Such a method needs
PH regulations are carried out to all samples in advance, and detected using the precise instruments such as UV-Vis scans spectrometers, no
It is only cumbersome, with high costs, not Portable belt, and be difficult to monitor in real time.On the other hand, current PNP degradation technique
Huge challenge be present, prior art is difficult to accomplish to degrade to it while pollutant is detected.
Microbiological fuel cell, it is the product that microbial technique is combined with battery technology, it is one kind by producing electricity bacterium
It is metabolized biodegradable organic matter, and by electron transmission caused by metabolism to external circuit and then exports the device of electric energy.At present
Research show that the voltage (electric current) of microbiological fuel cell and the concentration of substrate have corresponding relation in certain scope,
Therefore microbiological fuel cell can be used for the measure of some substrate contents.However, for microbiological fuel cell running status
Monitoring is still still unsolved problem in real time.At present, people are for substrate in the working level and battery of microbiological fuel cell
The measurement of concentration still relies on large-scale external device, not only wastes time and energy, and be unfavorable for popularization and application.
In addition, in recent years, miniaturization, cheap of the people for pollutant monitoring device, and it is remote for Monitoring Data
The needs of journey critical-path analysis are also more urgent.And the degradation of contaminant for being capable of low cost while pollutant is detected is even more then to have
It is significant.
The content of the invention：
In view of the above-mentioned problems, the technical problem to be solved in the present invention is to provide a kind of monitoring that can accurately automate is current
Organic pollution concentration, concentrate send out data be easy to subsequent analysis, more reduce pollutant detection and monitoring cost based on
The p-nitrophenol concentration detection method of microbiological fuel cell.
A kind of electro-chemical activity bacterium provided by the present invention, it derives from Lanzhou Huanghe section deposit, flat using dilution
Plate rubbing method is enriched with, and isolates and purifies to obtain, identified, the bacterium is Meng Shi pseudomonads (Pseudomonas monteili) LZU-
3, by China typical culture collection center (abbreviation CCTCC) preservation, preserving number is：CCTCC NO：M 2015454, preservation day
Phase is on July 15th, 2015, and preservation address is Chinese, Wuhan, Wuhan University.Bacterium colony of the bacterium on LB culture mediums is white,
Circle, surface is smooth, and moistening is sticky.The form that the bacterium is observed under ESEM is ellipse.Bacterial strain LZU-3 total length 16S
Accession number of the rDNA sequences in GenBank is KP056323.
A kind of p-nitrophenol concentration detection method based on microbiological fuel cell of the present invention, its hardware unit bag
Containing thermostat, cathode electrode (carbon felt/carbon brush), anode electrode (carbon felt/carbon brush), magnetic stirring apparatus, anode chamber, cathode chamber
(replaceable culture medium realizes the detection of other industrial wastewater pollution things), PEM, automatic sampling/sample mixing device, anode
Well, negative electrode well, anode pumping equipment, negative electrode pumping equipment, amplification chip, externally fed, ground connection, AD conversion,
MCU, input-output equipment；Magnetic stirring apparatus is equipped with anode chamber, cathode chamber, anode chamber, the centre of cathode chamber are provided with matter
Proton exchange, the both sides of PEM are equipped with thermostat, and the outside of anode chamber is provided with anode pumping equipment, cathode chamber
Outside is provided with negative electrode pumping equipment, is provided with anode electrode (carbon felt/carbon brush) in anode chamber, anode electrode (carbon felt/carbon brush) is with putting
The negative pole connection of large chip, cathode chamber is interior to be provided with cathode electrode (carbon felt/carbon brush), cathode electrode (carbon felt/carbon brush) and amplification core
The positive pole connection of piece, the side of amplification chip are connected with externally fed, and the opposite side of amplification chip is ground connection, amplification chip and AD
Conversion connection, AD conversion are connected with MCU, and MCU is connected with each other with input-output equipment, and MCU one end fills with automatic sampling/sample mixing
Connection is put, automatic sampling/sample mixing device is connected with anode well, and the MCU other end is connected with anode pumping equipment.
The improved culture medium prescription for anolyte in a whole set of solution provided by the invention is：K2HPO4·
3H2O 0.85g/L、KH2PO4 0.2g/L、MgSO4·7H2O 0.09g/L、FeSO4·7H2O0.0239g/L；Described negative electrode
Liquid composition is the K that the concentration prepared by 50mmol/L sodium phosphate buffers is 100mmol/L3[Fe(CN)6] mixed liquor.
As the expanded application of the Meng Shi pseudomonads in a whole set of solution provided by the invention, fired for microorganism
Produced electricity in material battery；Or the biosensor as organic pollutions such as PNP；Or determine for organic pollutions such as PNP
Property and quantitative detection；Or for organic pollutions such as the PNP that degrades；Or the thalline as freeze-drying is made resting cell and produced
Electricity；Or make the anode catalyst of microbiological fuel cell as bacterium absorption or the immobilized cell being embedded on electrode holder.
Detection method in a whole set of solution system provided by the invention, including following operating procedure：
First, device is run first：
1st, the K that the concentration prepared by 50mmol/L sodium phosphate buffers is 100mmol/L is injected to cathode chamber3[Fe(CN)6]
Mixed liquor 240mL；
2nd, 216mL MS culture mediums provided by the invention are injected to anode chamber；
3rd, and process MS washing cultured to anode chamber inoculation 24mL and again this hair of suspension (OD600=1.35)
The Meng Shi pseudomonads of bright offer；
4th, Meng Shi pseudomonads start to produce electricity, and electronics portion starts real-time monitoring voltage in system；
5th, when the voltage monitored drops to the default basic point of program (such as 30mV), step 6 is carried out；
6th, PNP titers (such as 250mL) known to a certain amount of concentration and a certain amount of are drawn in automatic sampling/sample mixing device
Mineral salts medium (Mineral Salts, the MS) dry powder of (being determined according to titer volume), carries out sample mixing；
7th, the electronics portion in system continues real-time monitoring voltage, and records and obtain adding PNP titers from this time
Add the maximum voltage value that anode back office box is monitored；
8th, the electronics portion in system judges whether complete according to user preset information or user's real-time command
Help the detection work of ministerial standard liquid.If not completing, anode pumping equipment is opened, after liquid emptying in anode chamber 5, is repeated
(returning to) step 5；If the judged result of electronic installation performs step 9 to have completed；
9th, the MCU in system is set according to each maximum voltage value recorded in above-mentioned steps, and by user by input
The PNP concentration of standard solution digital simulations that standby (18) provide draw formula；
2nd, the device run first has been completed, has carried out testing liquid PNP concentration monitors；
A, anode pumping equipment 11 is opened, after liquid emptying in anode chamber 5, automatic sampling/sample mixing device 8 is drawn necessarily
The solution to be measured (such as 240mL) of amount and a certain amount of (such as K2HPO4·3H2O 0.204g、KH2PO4 0.048、MgSO4·
7H2O 0.0216g、FeSO4·7H2O0.005736g mineral salts medium (Mineral Salts, MS) dry powder), is mixed
Sample；
B, the electronics portion in system continues real-time monitoring voltage, and records and obtain adding PNP titers from this time
The maximum voltage value that device is monitored afterwards；
C, the magnitude of voltage that MCU will be recorded in step B, substitute into the formula that step 9 obtains, and calculated, try to achieve step A
The concentration value of the solution to be measured of middle absorption；
D, the electronics portion in system shows result on an output device, and above-mentioned steps are obtained data,
Formula etc. uploads onto the server；
3rd, when voltage drops to basic point (such as 30mV), MCU determines to perform according to user preset condition, or real-time command
Step 4, or repeat step two, monitoring in real time is automated to realize；
4th, anode pumping equipment 11 is opened, after liquid emptying in anode chamber 5, closes system power supply.
Whole set of system provided by the invention has advantages below and beneficial effect：
1st, concentration that can be to be polluted in automatic measurement environment；2nd, there is simple to operate, equipment compared with traditional detection mode
The advantages of cost is low；3rd, there is the characteristics of portable compared with traditional detection mode；4th, can be in the same degradation of contaminant of detection；5、
Greatly optimize data final process and the form showed, it is possible to achieve more more equipment while remote monitoring；6th, can
Data are collected, convenient big data analysis later.
Brief description of the drawings：
For ease of explanation, the present invention is described in detail by following specific implementations and accompanying drawing.
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the schematic flow sheet of the method for inspection provided by the invention；
In figure：
1 thermostat；2 cathode electrodes (carbon felt/carbon brush)；3 anode electrodes (carbon felt/carbon brush)；4 magnetic stirring apparatuses；5 sun
Pole room；6 cathode chambers (replaceable culture medium realizes the detection of other industrial wastewater pollution things)；7 PEMs；8 automatic samplings/
Sample mixing device；9 anode wells；10 negative electrode wells；11 anode pumping equipments；12 negative electrode pumping equipments；13 amplification chips；14
Externally fed；15 ground connection；16AD is changed；17MCU；18 input-output equipment.
Embodiment：
As shown in Figure 1 to Figure 2, present embodiment uses following technical scheme：Its hardware unit fills comprising constant temperature
Put 1, cathode electrode (carbon felt/carbon brush) 2, anode electrode (carbon felt/carbon brush) 3, magnetic stirring apparatus 4, anode chamber 5, cathode chamber (can
Change culture medium and realize the detections of other industrial wastewater pollution things) 6, PEM 7, automatic sampling/sample mixing device 8, anode
Well 9, negative electrode well 10, anode pumping equipment 11, negative electrode pumping equipment 12, amplification chip 13, externally fed 14, ground connection
15th, AD conversion 16, MCU 17, input-output equipment 18；Magnetic stirring apparatus 4, anode chamber are equipped with anode chamber 5, cathode chamber 6
5th, the centre of cathode chamber 6 is provided with PEM 7, and the both sides of PEM 7 are equipped with thermostat 1, the outside of anode chamber 5
Provided with anode pumping equipment 11, the outside of cathode chamber 6 is provided with negative electrode pumping equipment 12, and anode electrode (carbon is provided with anode chamber 5
Felt/carbon brush) 3, anode electrode (carbon felt/carbon brush) 3 is connected with the positive pole of amplification chip 13, and cathode electrode (carbon is provided with cathode chamber 6
Felt/carbon brush) 2, cathode electrode (carbon felt/carbon brush) 2 is connected with the negative pole of amplification chip 13, and the side of amplification chip 13 supplies with outside
Electricity 14 connects, and the opposite side of amplification chip is ground connection 15, and amplification chip 13 is connected with AD conversion 16, and AD conversion 16 and MCU 17 connects
Connect, MCU 17 is connected with each other with input-output equipment 18, and MCU 17 one end is connected with automatic sampling/sample mixing device 8, is adopted automatically
Sample/sample mixing device 8 is connected with anode well 9, and MCU 17 other end is connected with anode pumping equipment 11.
Present embodiment provide a whole set of solution in the improved culture medium prescription for anolyte be：
K2HPO4·3H2O 0.85g/L、KH2PO4 0.2g/L、MgSO4·7H2O 0.09g/L、FeSO4·7H2O 0.0239g/L；Institute
The catholyte composition stated is the K that the concentration prepared by 50mmol/L sodium phosphate buffers is 100mmol/L3[Fe(CN)6] mixing
Liquid.
The expanded application of Meng Shi pseudomonads in a whole set of solution provided as present embodiment, is used for
Produced electricity in microbiological fuel cell；Or the biosensor as organic pollutions such as PNP；Or for organic dirt such as PNP
Contaminate the qualitative and quantitative detection of thing；Or for organic pollutions such as the PNP that degrades；Or that tranquillization is made is thin for the thalline as freeze-drying
Born of the same parents are produced electricity；Or make the anode of microbiological fuel cell as bacterium absorption or the immobilized cell being embedded on electrode holder
Catalyst.
Detection method in a whole set of solution system that present embodiment provides, including following operating procedure：
First, device is run first：
1st, the K that the concentration prepared by 50mmol/L sodium phosphate buffers is 100mmol/L is injected to cathode chamber3[Fe(CN)6]
Mixed liquor 240mL；
2nd, to the good OD600=1.35 of the inoculated and cultured of anode chamber 5 Meng Shi pseudomonads provided by the invention
(Pseudomonas monteilii)；
3rd, 50mg/L PNP titers are added from anode well 9, start battery；
4th, Meng Shi pseudomonads start to produce electricity, and electronics portion starts real-time monitoring voltage in system；
5th, when the voltage monitored drops to the default basic point of program (such as 30mV), step 6 is carried out；
6th, PNP titers (such as 250mL) known to a certain amount of concentration and a certain amount of are drawn in automatic sampling/sample mixing device
Mineral salts medium (Mineral Salts, the MS) dry powder of (being determined according to titer volume), carries out sample mixing；
7th, the electronics portion in system continues real-time monitoring voltage, and records and obtain adding PNP titers from this time
Add the maximum voltage value that anode back office box is monitored；
8th, the electronics portion in system judges whether complete according to user preset information or user's real-time command
Help the detection work of ministerial standard liquid.If not completing, anode pumping equipment is opened, after liquid emptying in anode chamber 5, is repeated
(returning to) step 6；If the judged result of electronic installation performs step 9 to have completed；
9th, the MCU in system is set according to each maximum voltage value recorded in above-mentioned steps, and by user by input
The PNP concentration of standard solution digital simulations that standby (18) provide draw formula；
2nd, the device run first has been completed, has carried out testing liquid PNP concentration monitors；
A, anode pumping equipment 11 is opened, after liquid emptying in anode chamber 5, automatic sampling/sample mixing device 8 is drawn necessarily
The solution to be measured (such as 240mL) of amount and a certain amount of (such as K2HPO4·3H2O 0.204g、KH2PO4 0.048、MgSO4·
7H2O 0.0216g、FeSO4·7H2O0.005736g mineral salts medium (Mineral Salts, MS) dry powder), is mixed
Sample；
B, the electronics portion in system continues real-time monitoring voltage, and records and obtain adding PNP titers from this time
The maximum voltage value that device is monitored afterwards；
C, the formula that MCU obtains according to step 9 substitutes into step B the magnitude of voltage recorded, and is calculated, and tries to achieve step A
The concentration value of the solution to be measured of middle absorption；
D, the electronics portion in system shows result on an output device, and above-mentioned steps are obtained data,
Formula etc. uploads onto the server；
3rd, when voltage drops to basic point (such as 30mV), MCU determines to perform according to user preset condition, or real-time command
Step 4, or repeat step two, monitoring in real time is automated to realize；
4th, anode pumping equipment 11 is opened, after liquid emptying in anode chamber 5, closes system power supply.
Wherein, described digital voltage-stabilizing system is one piece of Whole PC B circuit.The circuit amplification system has made PCB
Plank；The A/D convertor circuit is also made as fixed PCB and MCU puts together；Single-chip microcomputer pcDuino is internally integrated
8 analog-digital converters, power module include one piece of 9V battery case and linear voltage regulator TPS5430 modules；Input and output are set
Standby is one piece of 7inch 1024x600LCD, can show the color graphics of 1024x600 resolution ratio；Present embodiment carries
Meng Shi pseudomonads in the whole set of system of confession have the characteristic that can experience PNP concentration, in 9-36mg/L PNP concentration models
In enclosing, thalline electricity production has notable linear relationship with PNP concentration.
Present embodiment can be to be polluted in automatic measurement environment concentration, compared with traditional detection mode have operation
Simply, the advantages of equipment cost is low, there is the characteristics of portable compared with traditional detection mode, can be in the same pollution degradation of detection
Thing, greatly optimize data final process and the form showed, it is possible to achieve more more equipment while remote monitoring, can
Data are collected, convenient big data analysis later.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The skill of the industry
For art personnel it should be appreciated that the present invention is not limited to the above embodiments, described in above-described embodiment and specification is explanation
The principle of the present invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these
Changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and
Its equivalent thereof.
Claims (2)
- A kind of 1. p-nitrophenol concentration detection method based on microbiological fuel cell, it is characterised in that：One kind that it is provided Electro-chemical activity bacterium, it derives from Lanzhou Huanghe section deposit, is enriched with using dilution plate rubbing method, isolates and purifies to obtain, Identified, the bacterium is Meng Shi pseudomonad LZU-3, and by China typical culture collection center preservation, preserving number is：CCTCC NO：M2015454, preservation date are on July 15th, 2015, and preservation address is Chinese, Wuhan, Wuhan University；The bacterium is cultivated in LB Bacterium colony on base is white, and circular, surface is smooth, and moistening is sticky；The form that the bacterium is observed under ESEM is ellipse；Bacterial strain Accession number of the LZU-3 total length 16S rDNA sequences in GenBank is KP056323；Its detection method includes following operation Step：(1), device is run first：(1) K that the concentration prepared by 50mmol/L sodium phosphate buffers is 100mmol/L, is injected to cathode chamber3[Fe(CN)6] mixed Close liquid 240mL；(2), 216mLMS culture mediums is injected to anode chamber(3) the Meng Shi provided by the invention vacations that 24mL is cultured and washs by MS and suspends again, are inoculated with to anode chamber Monad；(4), Meng Shi pseudomonads start to produce electricity, and electronics portion starts real-time monitoring voltage in system；(5), when the voltage monitored drops to the default basic point of program, step (6) is carried out；(6), automatic sampling/sample mixing device draws PNP titers and a certain amount of MS culture medium dry powders known to a certain amount of concentration, Carry out sample mixing；(7), the electronics portion in system continues real-time monitoring voltage, and records and obtain adding from this time addition PNP titers Enter the maximum voltage value that anode back office box is monitored；(8), the electronics portion in system judges whether to have completed according to user preset information or user's real-time command The detection work of whole titers；If not completing, anode pumping equipment is opened, after anode indoor liquid emptying, is returned to Step (6)；If the judged result of electronic installation performs step (9) to have completed；(9), the MCU in system passes through input equipment according to each maximum voltage value recorded in above-mentioned steps, and by user The PNP concentration of standard solution digital simulations of offer draw formula；(2) device run first, has been completed, has carried out testing liquid PNP concentration monitors；(A) anode pumping equipment, is opened, after anode indoor liquid emptying, automatic sampling/sample mixing device draws a certain amount of treat Solution and a certain amount of MS culture medium dry powders are surveyed, carries out sample mixing；(B), the electronics portion in system continues real-time monitoring voltage, and records and obtain after this time adds PNP titers The maximum voltage value that device is monitored；(C), the formula that MCU obtains according to step (9) substitutes into the magnitude of voltage recorded in step (B), and is calculated, and tries to achieve step (A) concentration value for the solution to be measured drawn in；(D), the electronics portion in system shows result on an output device, and data, the public affairs that above-mentioned steps are obtained Formula etc. uploads onto the server；(3), when voltage drops to basic point, MCU determines to perform step 4 according to user preset condition, or real-time command, or repeats Step 2, monitoring in real time is automated to realize；(4) anode pumping equipment, is opened, after anode indoor liquid emptying, closes system power supply.
- 2. a kind of p-nitrophenol concentration detection method based on microbiological fuel cell according to claim 1, it is special Sign is that the improved culture medium prescription for anolyte is：K2HPO4·3H2O0.85g/L、KH2PO40.2g/L、MgSO4· 7H2O 0.09g/L、FeSO4·7H2O 0.0239g/L；Described catholyte composition is to be matched somebody with somebody by 50mmol/L sodium phosphate buffers The concentration of system is 100mmol/L K3[Fe(CN)6] mixed liquor.
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| CN102906246A (en) * | 2010-02-23 | 2013-01-30 | 贝克蒂瑞罗博蒂克斯有限责任公司 | Improved microbial fuel cell |
| CN104330455A (en) * | 2014-07-16 | 2015-02-04 | 内蒙古科技大学 | Method and device of on-line monitoring nitrate nitrogen through microbial electrolytic tank technology |
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| US8354267B2 (en) * | 2009-02-23 | 2013-01-15 | Bacterial Robotics, Llc | Microbial fuel cell |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102906246A (en) * | 2010-02-23 | 2013-01-30 | 贝克蒂瑞罗博蒂克斯有限责任公司 | Improved microbial fuel cell |
| CN104330455A (en) * | 2014-07-16 | 2015-02-04 | 内蒙古科技大学 | Method and device of on-line monitoring nitrate nitrogen through microbial electrolytic tank technology |
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