CN201623198U - Anammox microbial fuel cell - Google Patents
Anammox microbial fuel cell Download PDFInfo
- Publication number
- CN201623198U CN201623198U CN 201020139304 CN201020139304U CN201623198U CN 201623198 U CN201623198 U CN 201623198U CN 201020139304 CN201020139304 CN 201020139304 CN 201020139304 U CN201020139304 U CN 201020139304U CN 201623198 U CN201623198 U CN 201623198U
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- Prior art keywords
- biofilm
- reactor body
- tubular reactor
- negative electrode
- anode
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/307—Nitrification and denitrification treatment characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process
Abstract
The utility model discloses an anammox (anaerobic ammonium oxidation) microbial fuel cell comprising a constant-temperature stirring system, a reaction system and a data acquisition and monitoring system, wherein the constant-temperature stirring system comprises a constant-temperature magnetic stirrer body and a magnetic stirring bar; the reaction system comprises a cylindrical reactor body and a sealing cover; a water inlet pipe and a water outlet pipe are respectively arranged at the lower part and the upper part of the cylindrical reactor body; an electrogenesis substrate is mounted inside the cylindrical reactor body; free anammox bacteria, and a biofilm-culturing anode and a biofilm-culturing cathode, to which the anammox bacteria are attached, are immersed in the electrogenesis substrate; an anode wire fixing pipe, a cathode wire fixing pipe, a sampling pipe and a reference electrode fixing pipe are arranged at the upper part of the sealing cover; a sealing ring is arranged between the cylindrical reactor body and the sealing cover which are connected by a flange; and the data acquisition and monitoring system comprises a load, wires, a data acquisition unit and an on-line computer. The anammox microbial fuel cell of the utility model has the advantages of simple structure, low construction cost, low internal resistance, high efficiency and stable performance and is capable of achieving the nitrogen removal from wastewater and the biological electrogenesis synchronously.
Description
Technical field
The utility model relates to biological fuel cell, relates in particular to a kind of anaerobic ammonia oxidation microbiological fuel cell.
Background technology
Microbiological fuel cell (Microbial Fuel Cells, be called for short MFC) be a kind of be anode catalyst with the microbe, chemical energy is converted into the device of electric energy.Utilize microbiological fuel cell, not only can be directly with the organic matter degradation in water body or the mud, and the electronics that produces in the organic metabolism process can be converted into electric current, thus obtain electric energy.In recent years, under the dual-pressure of environmental pollution and energy crisis, because microbiological fuel cell can be handled waste water simultaneously and produce electric energy, this new technology is favored by people more and more, has become the research focus in waste water treatment and new energy development field.
The operation principle of microbiological fuel cell is as follows: under the anode action of microorganisms, substrate decomposes generation electronics, proton and other metabolite; Portions of electronics is delivered to anode surface and arrives negative electrode by external circuit, and proton arrives negative electrode through electrogenesis matrix; At cathode surface, electronics, proton combine and form electric current with electron acceptor.Most microbiological fuel cells are electrogenesis matrix with the organic substance, and the electrogenesis microbe is a facultative anaerobe, and battery configuration has two chambers and single chamber two classes.There is following shortcoming in these microbiological fuel cells: (1) is in the amphimicrobian electrogenesis bacterium of having found, except that corrupt Shiva Salmonella (Shewanella putrefaciens), bacillus (Geobacteraceae), the iron vat red spirillum several bacteriums such as (Rhodoferax ferrireducens), the electron transport efficient of all the other electrogenesis bacterium is very low, need the extra dimethyl diaminophenazine chloride, 2 of adding, electron mediums such as 6-anthraquinone come the strengthening electronic transmission, and mediator is poisonous mostly and price is higher.(2) double-chamber microbiological fuel cell is divided into two utmost point chambers of yin, yang by proton exchange membrane, the proton transfer ability of proton exchange membrane can change the pH value of cathode and anode chamber, weaken microbial activity and electron transport ability, and proton exchange membrane costs an arm and a leg (4000～12000 yuans/m
2); The electronics that is delivered to negative electrode need add the electron acceptor combination, electron acceptor has two kinds of supply modes, a kind of mode is to utilize the iron cyanide and permanganate etc. to have the chemical substance of high oxidation activity, this method need not supported precious metal catalyst on negative electrode, but cost height, regeneration be difficult, easily cause secondary pollution, another kind of mode is as electron acceptor, though air is cheap and easy to get with air, but its redox speed is low, needs at noble metal catalysts such as cathode load platinum.(3) single-chamber microbial fuel cell does not have cathode chamber, but still needs proton exchange membrane, and its negative electrode also needs supported precious metal catalyst but also needs complicated water-proofing treatment.(4) electrogenesis matrix is organic substance, and conductivity is low, and the internal resistance of cell is big, and electrogenesis efficient is low, needs additionally to add inorganic ions sometimes to strengthen its conductivity.The two big bottlenecks that electrogenesis efficient is low and the cost height has become the existing microbiological fuel cell development of restriction and used.
At the above-mentioned defective of existing microbiological fuel cell, the utility model is the electrogenesis bacterium with the anaerobic ammonia oxidizing bacteria, need not to add electron medium; With the inorganic wastewater that contains ammonia nitrogen and nitrite nitrogen is electrogenesis matrix, and yin, yang the two poles of the earth are in the homophase solution, need not to be provided with proton exchange membrane; Nitrite can need not to provide in addition the cathode electronics acceptor directly as the cathode electronics acceptor in the electrogenesis matrix, and negative electrode adheres to the electrogenesis biomembrane, can improve electron transport efficient, need not supported precious metal catalyst; Electrogenesis matrix is inorganic ions, and the internal resistance of cell is low, and battery performance is good, electrogenesis efficient height.
Summary of the invention
The purpose of this utility model is to overcome the deficiencies in the prior art, and a kind of anaerobic ammonia oxidation microbiological fuel cell is provided.
Anaerobic ammonia oxidation microbiological fuel cell is made up of constant temperature stirring system, reaction system and data acquisition and monitoring system three parts, and the constant temperature stirring system is provided with constant temperature blender with magnetic force body and magnetic stir bar; Reaction system is provided with tubular reactor body and seal cover, tubular reactor body bottom is provided with water inlet pipe, tubular reactor body top is provided with outlet pipe, electrogenesis matrix is equipped with in tubular reactor body inside, be soaked with free anaerobic ammonia oxidizing bacteria and the biofilm anode and the biofilm negative electrode that adhere to anaerobic ammonia oxidizing bacteria in the electrogenesis matrix, seal cover top is provided with positive wire stationary pipes, cathode wire stationary pipes, probe tube and reference electrode stationary pipes, is provided with sealing ring between tubular reactor body and the seal cover and connects by flange; The data acquisition and monitoring system is provided with load, lead, data acquisition unit and line computer, and the load two ends link to each other with the biofilm negative electrode with the biofilm anode respectively by lead.
Described electrogenesis matrix is the inorganic wastewater that contains ammonia nitrogen and nitrite nitrogen, and the pH value is 7.0～7.5, wherein adds microelement concentrate I, each 1.25mL/L of microelement concentrate II, and the composition of microelement concentrate I is: EDTA 5g/L, FeSO
45g/L; The composition of microelement concentrate II is: EDTA 15g/L, H
3BO
40.014g/L, MnCl
24H
2O 0.99g/L, CuSO
45H
2O 0.025g/L, ZnSO
47H
2O 0.43g/L, NiCl
26H
2O 0.19g/L, NaSeO
410H
2O 0.21g/L, NaMoO
42H
2O 0.22g/L.
Described tubular reactor body ratio of height to diameter is 1～2: 1, and electrogenesis matrix volume accounts for 2/3～3/4 of tubular reactor body volume, and free anaerobic ammonia oxidizing bacteria volume accounts for 1/5～1/10 of electrogenesis matrix volume.
The electric conducting material of described biofilm anode and biofilm negative electrode is carbon paper, carbon cloth, carbon felt, graphite felt or graphite cake, biofilm anode and biofilm cathode surface are attached with the anaerobic ammonia oxidizing bacteria biomembrane, end distance tubular reactor body bottom is 2～4cm under biofilm anode and the biofilm negative electrode, distance between biofilm anode and the biofilm negative electrode is 3～6cm, and biofilm anode or biofilm cathode area are 9～40m with the ratio of tubular reactor body volume
2: 1m
3
The beneficial effect that the utlity model has: (1) utilizes anaerobic ammonia oxidizing bacteria to carry out denitrogenation of waste water and biological electrogenesis, can realize that contaminated wastewater is controlled and electrical energy production is carried out simultaneously.(2) yin, yang the two poles of the earth are in the homophase solution, need not to add electron medium, need not proton exchange membrane, and nitrite can be directly as the cathode electronics acceptor, and apparatus structure is simple, are easy to amplify and industrial applications.(3) electrogenesis matrix is inorganic ions, and the internal resistance of cell is low, and battery performance is good, and electrogenesis efficient height does not discharge the greenhouse gases carbon dioxide in the electricity generation process, is a kind of clean energy resource process units.
Description of drawings
Accompanying drawing 1 is a kind of anaerobic ammonia oxidizing bacteria microbiological fuel cell structural representation;
Accompanying drawing 2 is structural representations of seal cover;
Wherein: constant temperature blender with magnetic force body 1, magnetic stir bar 2, water inlet pipe 3, free anaerobic ammonia oxidizing bacteria 4, biofilm anode 5, biofilm negative electrode 6, electrogenesis matrix 7, tubular reactor body 8, seal cover 9, flange 10, positive wire stationary pipes 11, probe tube 12, load 13, reference electrode stationary pipes 14, cathode wire stationary pipes 15, sealing ring 16, outlet pipe 17, lead 18, data acquisition unit 19, line computer 20.
Embodiment
As shown in drawings, anaerobic ammonia oxidation microbiological fuel cell is made up of constant temperature stirring system I, reaction system II and data acquisition and monitoring system III three parts, and constant temperature stirring system I is provided with constant temperature blender with magnetic force body 1 and magnetic stir bar 2; Reaction system II is provided with tubular reactor body 8 and seal cover 9, tubular reactor body 8 bottoms are provided with water inlet pipe 3, tubular reactor body 8 tops are provided with outlet pipe 17, electrogenesis matrix 7 is equipped with in tubular reactor body 8 inside, be soaked with free anaerobic ammonia oxidizing bacteria 4 and the biofilm anode 5 and the biofilm negative electrode 6 that adhere to anaerobic ammonia oxidizing bacteria in the electrogenesis matrix 7, seal cover 9 tops are provided with positive wire stationary pipes 11, cathode wire stationary pipes 15, probe tube 12 and reference electrode stationary pipes 14, are provided with sealing ring 16 between tubular reactor body 8 and the seal cover 9 and pass through flange 10 to connect; The III of data acquisition and monitoring system is provided with load 13, lead 18, data acquisition unit 19 and line computer 20, and load 13 two ends link to each other with biofilm negative electrode 6 with biofilm anode 5 respectively by lead 18.
Described electrogenesis matrix 7 is for containing the inorganic wastewater of ammonia nitrogen and nitrite nitrogen, and the pH value is 7.0～7.5, wherein adds microelement concentrate I, each 1.25mL/L of microelement concentrate II, and the composition of microelement concentrate I is: EDTA 5g/L, FeSO
45g/L; The composition of microelement concentrate II is: EDTA 15g/L, H
3BO
40.014g/L, MnCl
24H
2O 0.99g/L, CuSO
45H
2O 0.025g/L, ZnSO
47H
2O0.43g/L, NiCl
26H
2O 0.19g/L, NaSeO
410H
2O 0.21g/L, NaMoO
42H
2O 0.22g/L.
Described tubular reactor body 8 ratio of height to diameters are 1～2: 1, and electrogenesis matrix 7 volumes account for 2/3～3/4 of tubular reactor body 8 volumes, and free anaerobic ammonia oxidizing bacteria 4 volumes account for 1/5～1/10 of electrogenesis matrix 7 volumes.
The electric conducting material of described biofilm anode 5 and biofilm negative electrode 6 is carbon paper, carbon cloth, carbon felt, graphite felt or graphite cake, biofilm anode 5 and biofilm negative electrode 6 surface attachment have the anaerobic ammonia oxidizing bacteria biomembrane, biofilm anode 5 and 6 times end distance tubulars of biofilm negative electrode reactor body, 8 bottoms are 2～4cm, distance between biofilm anode 5 and the biofilm negative electrode 6 is 3～6cm, and biofilm anode 5 or biofilm negative electrode 6 areas are 9～40m with the ratio of tubular reactor body 8 volumes
2: 1m
3
Open the constant temperature stirring system, anaerobic ammonia oxidizing bacteria is dispersed under stirring action in the tubular reactor body with the inorganic wastewater that contains ammonia nitrogen and nitrite nitrogen, and constant temperature can be the Anammox bacteria growing and the reaction of denitrogenation electrogenesis provides suitable temperature conditions.Ammonia nitrogen discharges electronics and proton under the effect of anaerobic ammonia oxidizing bacteria, the electronics that discharges is collected by the biofilm anode and is delivered to the biofilm negative electrode by the external circuit lead, at the biofilm cathode surface, electronics, proton and nitrite combination produce electric current and generate nitrogen, finish denitrogenation and electricity generation process thus, the signal of telecommunication that reaction system produces is collected by data acquisition unit, and be transferred in the line computer, by the change in electric ruuning situation of monitoring reaction system simultaneously, the process of being convenient to is in time regulated and control.
Claims (4)
1. anaerobic ammonia oxidation microbiological fuel cell, it is characterized in that it is made up of constant temperature stirring system (I), reaction system (II) and data acquisition and monitoring system (III) three parts, constant temperature stirring system (I) is provided with constant temperature blender with magnetic force body (1) and magnetic stir bar (2); Reaction system (II) is provided with tubular reactor body (8) and seal cover (9), tubular reactor body (8) bottom is provided with water inlet pipe (3), tubular reactor body (8) top is provided with outlet pipe (17), electrogenesis matrix (7) is equipped with in tubular reactor body (8) inside, be soaked with free anaerobic ammonia oxidizing bacteria (4) in the electrogenesis matrix (7) and adhere to the biofilm anode (5) and the biofilm negative electrode (6) of anaerobic ammonia oxidizing bacteria, seal cover (9) top is provided with positive wire stationary pipes (11), cathode wire stationary pipes (15), probe tube (12) and reference electrode stationary pipes (14) are provided with sealing ring (16) and pass through flange (10) connection between tubular reactor body (8) and the seal cover (9); Data acquisition and monitoring system (III) is provided with load (13), lead (18), data acquisition unit (19) and line computer (20), and load (13) two ends link to each other with biofilm negative electrode (6) with biofilm anode (5) respectively by lead (18).
2. a kind of anaerobic ammonia oxidation microbiological fuel cell according to claim 1, it is characterized in that described electrogenesis matrix (7) is for containing the inorganic wastewater of ammonia nitrogen and nitrite nitrogen, the pH value is 7.0～7.5, wherein add microelement concentrate I, each 1.25mL/L of microelement concentrate II, the composition of microelement concentrate I is: EDTA 5g/L, FeSO
45g/L; The composition of microelement concentrate II is: EDTA 15g/L, H
3BO
40.014g/L, MnCl
24H
2O 0.99g/L, CuSO
45H
2O 0.025g/L, ZnSO
47H
2O 0.43g/L, NiCl
26H
2O 0.19g/L, NaSeO
410H
2O 0.21g/L, NaMoO
42H
2O 0.22g/L.
3. a kind of anaerobic ammonia oxidation microbiological fuel cell according to claim 1, it is characterized in that described tubular reactor body (8) ratio of height to diameter is 1～2: 1, electrogenesis matrix (7) volume accounts for 2/3～3/4 of tubular reactor body (8) volume, and free anaerobic ammonia oxidizing bacteria (4) volume accounts for 1/5～1/10 of electrogenesis matrix (7) volume.
4. a kind of anaerobic ammonia oxidation microbiological fuel cell according to claim 1, the electric conducting material that it is characterized in that described biofilm anode (5) and biofilm negative electrode (6) is a carbon paper, carbon cloth, the carbon felt, graphite felt or graphite cake, biofilm anode (5) and biofilm negative electrode (6) surface attachment have the anaerobic ammonia oxidizing bacteria biomembrane, biofilm anode (5) and biofilm negative electrode (6) end distance tubular reactor body (8) bottom down are 2～4cm, distance between biofilm anode (5) and the biofilm negative electrode (6) is 3～6cm, and the ratio of biofilm anode (5) or biofilm negative electrode (6) area and tubular reactor body (8) volume is 9～40m
2: 1m
3
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020139304 CN201623198U (en) | 2010-03-23 | 2010-03-23 | Anammox microbial fuel cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020139304 CN201623198U (en) | 2010-03-23 | 2010-03-23 | Anammox microbial fuel cell |
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| Publication Number | Publication Date |
|---|---|
| CN201623198U true CN201623198U (en) | 2010-11-03 |
Family
ID=43026655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020139304 Expired - Lifetime CN201623198U (en) | 2010-03-23 | 2010-03-23 | Anammox microbial fuel cell |
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|---|---|
| CN (1) | CN201623198U (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101794896A (en) * | 2010-03-23 | 2010-08-04 | 浙江大学 | Anaerobic ammonia oxidation microbiological fuel cell |
| CN102219299A (en) * | 2011-04-01 | 2011-10-19 | 中国科学院成都生物研究所 | Wastewater anaerobic oxidation and negative oxygen ion coupled generation device and method |
| CN102290590A (en) * | 2011-07-28 | 2011-12-21 | 清华大学 | Biocathode microbial fuel cell |
| CN102786330A (en) * | 2012-08-24 | 2012-11-21 | 哈尔滨工业大学 | System for accelerating anaerobic composting of dewatered sludge by bioelectricity production |
| CN105906051A (en) * | 2016-06-06 | 2016-08-31 | 山东大学 | Electrochemical microorganism autotrophic nitrogen removal sewage treatment method and system |
| CN105948222A (en) * | 2016-06-23 | 2016-09-21 | 浙江大学 | Anaerobic digestion, denitrification and anaerobic ammonium oxidation bioelectrochemical system and method |
| CN108840449A (en) * | 2018-07-06 | 2018-11-20 | 武汉轻工大学 | Sewage disposal device and sewage water treatment method based on membrane bioreactor |
| CN109264850A (en) * | 2018-11-09 | 2019-01-25 | 河北大学 | Integral anaerobic ammoxidation film bioelectrochemical system and sewage water denitrification remove the treatment process of carbon |
| CN109638327A (en) * | 2018-12-19 | 2019-04-16 | 大连理工大学 | A kind of technique carrying out denitrogenation electricity production with single chamber anaerobic ammonium oxidation sludge-microbial fuel cell unit |
-
2010
- 2010-03-23 CN CN 201020139304 patent/CN201623198U/en not_active Expired - Lifetime
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101794896B (en) * | 2010-03-23 | 2012-06-13 | 浙江大学 | Anaerobic ammonia oxidation microbiological fuel cell |
| CN101794896A (en) * | 2010-03-23 | 2010-08-04 | 浙江大学 | Anaerobic ammonia oxidation microbiological fuel cell |
| CN102219299A (en) * | 2011-04-01 | 2011-10-19 | 中国科学院成都生物研究所 | Wastewater anaerobic oxidation and negative oxygen ion coupled generation device and method |
| CN102219299B (en) * | 2011-04-01 | 2012-12-19 | 中国科学院成都生物研究所 | Wastewater anaerobic oxidation and negative oxygen ion coupled generation device and method |
| CN102290590A (en) * | 2011-07-28 | 2011-12-21 | 清华大学 | Biocathode microbial fuel cell |
| CN102290590B (en) * | 2011-07-28 | 2013-07-24 | 清华大学 | Biocathode microbial fuel cell |
| CN102786330A (en) * | 2012-08-24 | 2012-11-21 | 哈尔滨工业大学 | System for accelerating anaerobic composting of dewatered sludge by bioelectricity production |
| CN105906051B (en) * | 2016-06-06 | 2019-01-18 | 山东大学 | A kind of electrochemistry microorganism autotrophic denitrification sewage water treatment method and system |
| CN105906051A (en) * | 2016-06-06 | 2016-08-31 | 山东大学 | Electrochemical microorganism autotrophic nitrogen removal sewage treatment method and system |
| CN105948222A (en) * | 2016-06-23 | 2016-09-21 | 浙江大学 | Anaerobic digestion, denitrification and anaerobic ammonium oxidation bioelectrochemical system and method |
| CN105948222B (en) * | 2016-06-23 | 2018-09-07 | 浙江大学 | Anaerobic digestion denitrification anaerobic ammoxidation bioelectrochemical system and method |
| CN108840449A (en) * | 2018-07-06 | 2018-11-20 | 武汉轻工大学 | Sewage disposal device and sewage water treatment method based on membrane bioreactor |
| CN109264850A (en) * | 2018-11-09 | 2019-01-25 | 河北大学 | Integral anaerobic ammoxidation film bioelectrochemical system and sewage water denitrification remove the treatment process of carbon |
| CN109264850B (en) * | 2018-11-09 | 2023-10-13 | 河北大学 | Integrated anaerobic ammonia oxidation membrane bioelectrochemical system and sewage denitrification and decarbonization treatment process |
| CN109638327A (en) * | 2018-12-19 | 2019-04-16 | 大连理工大学 | A kind of technique carrying out denitrogenation electricity production with single chamber anaerobic ammonium oxidation sludge-microbial fuel cell unit |
| CN109638327B (en) * | 2018-12-19 | 2021-05-18 | 大连理工大学 | Process for denitrification and power generation by using single-chamber anaerobic ammonia oxidation sludge-microbial fuel cell device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20101103 Effective date of abandoning: 20120613 |