CN105548587A - Multifunctional micro fluidic intelligent experiment device and work method thereof - Google Patents
Multifunctional micro fluidic intelligent experiment device and work method thereof Download PDFInfo
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- CN105548587A CN105548587A CN201510870131.XA CN201510870131A CN105548587A CN 105548587 A CN105548587 A CN 105548587A CN 201510870131 A CN201510870131 A CN 201510870131A CN 105548587 A CN105548587 A CN 105548587A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
- G01N2035/00376—Conductive heating, e.g. heated plates
Abstract
The invention relates to a multifunctional micro fluidic intelligent experiment device and a work method thereof. The intelligent experiment device comprises a card slot, into which a micro fluidic chip can be inserted, wherein the card slot is arranged on a vibration mechanism, which is controlled by a processor module; a heating device, which is arranged in the card slot and is controlled by the processor module; and a sampling device, which is used to sample the reaction liquid in the micro fluidic chip.
Description
Technical field
The present invention relates to a kind of microfluidic experimental device, particularly relate to a kind of multifunctional microflow control intelligence experimental provision and method of work thereof.
Background technology
Biochemistry detection micro-fluidic chip is multifunction system chip, this chip is formed in integrated for the basic operation units such as the sample preparation involved by biochemistry detection, quantitative sample injection, liquid mixing, biochemical reaction, separation detection or baseset on the chip of several square centimeters, is a kind of technology platform of the various functions replacing conventional chemical or biology laboratory.
Traditional micro-fluidic intelligent experimental provision function is very single, cannot realize intellectualized operation, therefore, the invention provides a kind of multifunctional microflow control intelligence experimental provision and method of work thereof.
Summary of the invention
The object of this invention is to provide a kind of multifunctional microflow control intelligence experimental provision and method of work thereof, this micro-fluidic intelligent experimental provision is suitable in the environment of sealing, pass through vibration, heating realizes the rapid reaction of reagent, and can sample reactant liquor, improve conventional efficient.
In order to solve the problems of the technologies described above, the invention provides a kind of multifunctional microflow control intelligence experimental provision of micro-fluidic chip, be suitable for the draw-in groove that micro-fluidic chip vertically inserts, described draw-in groove is arranged on a vibrating mechanism, and described vibrating mechanism is controlled by processor module; And in the groove of draw-in groove, being provided with the heating arrangement controlled by described processor module, this heating arrangement is suitable for heating micro-fluidic chip domestic demand reaction reagent; And described micro-fluidic intelligent experimental provision also comprises the sampler being suitable for sampling reactant liquor in micro-fluidic chip.
Further, described multifunctional microflow control intelligence experimental provision also comprises: be suitable for the draw-in groove that micro-fluidic chip vertically inserts, for the supply module of micro-fluidic chip heating, and be positioned at the pressurizing unit of described draw-in groove side; Described supply module, pressurizing unit control by processor module, and described processor module is also connected with a temperature sensor, with after micro-fluidic chip is heated to uniform temperature, control pressurizing unit transverse shear stress micro-fluidic chip.
Further, described micro-fluidic chip adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer, intermediate connecting layer, the hybrid reaction layer that are arranged in order; Distribute in described reagent reserve liquid layer some liquid storage tanks, and each liquid storage tank is by being covered in diaphragm seal and the intermediate connecting layer sealing on agent reserve liquid layer surface; Described intermediate connecting layer is provided with the through hole of liquid storage tank discharge opeing, and through hole is sealed by paraffin, when paraffin melting, liquid storage tank is connected with each microchannel branch road in hybrid reaction layer by through hole, the downward-sloping microchannel main road connected respectively in hybrid reaction layer of each microchannel branch road, this microchannel main road is communicated with the reaction tank be positioned at bottom hybrid reaction layer vertically downward; And the height of each liquid storage tank is all higher than reaction tank; And the height of each liquid storage tank is all higher than reaction tank, namely reaction tank is positioned at the bottom of micro-fluidic chip; The described reaction reagent that needs that described heating arrangement is suitable for being arranged in reaction tank is heated; Further, described hybrid reaction layer is also provided with a pipeline being suitable for microchannel main road to communicate with the external world, and this line portals is coated with sealing film, at medicament after completion of the reaction, tears sealing film, and described sampler extracts reactant liquor out chemical examination by inserting needle tubing; Described sampler comprises the negative pressure cavity be connected with needle tubing, namely by needle tubing by reactant liquor this negative pressure cavity of suction from reaction tank.
Further, in described intermediate connecting layer, be inlaid with heater strip, and each heater strip is set around sealing place of corresponding paraffin respectively; The multi-channel output of described supply module is connected with the energization input of each heater strip respectively, and this supply module controls multiple-channel output by processor module; Heater strip electrified regulation is controlled, to melt paraffin by described processor module.
Further, described pressurizing unit adopts air curtain, and is provided with some gas orifices in air curtain, and each gas orifice is respectively to corresponding accurate liquid storage tank position; The steam line of each gas orifice is respectively equipped with air valve, and the control end of each air valve is all connected with processor module, control corresponding air valve by described processor module to open, gas orifice aims at liquid storage tank position ejection gas, to oppress liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
Further, described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod is driven by corresponding screw body respectively, namely stretches out compressing liquid storage tank, and make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast; The drive motor of described each screw body controls by described processor module.
Another aspect, present invention also offers a kind of method of work of described multifunctional microflow control intelligence experimental provision, comprises the steps:
Step S1, heats micro-fluidic chip;
Step S2, controls pressurizing unit transverse shear stress micro-fluidic chip;
Step S3, Vibration on Start-up mechanism, carries out dither to micro-fluidic chip, and heats micro-fluidic chip domestic demand reaction reagent, makes its sufficient reacting; And
Step S4, is chemically examined by sampler abstraction reaction liquid from micro-fluidic chip.
Further, described multifunctional microflow control intelligence experimental provision comprises: for the supply module of micro-fluidic chip heating, and be positioned at the pressurizing unit of described draw-in groove side; Described supply module, pressurizing unit control by processor module, and described processor module is also connected with a temperature sensor; In described step S1, the method for micro-fluidic chip heating is comprised: control supply module by processor module and micro-fluidic chip is heated.
Further, described micro-fluidic chip adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer, intermediate connecting layer, the hybrid reaction layer that are arranged in order; Distribute in described reagent reserve liquid layer some liquid storage tanks, and each liquid storage tank is by being covered in diaphragm seal and the intermediate connecting layer sealing on agent reserve liquid layer surface; Described intermediate connecting layer is provided with the through hole of liquid storage tank discharge opeing, and through hole is sealed by paraffin, when paraffin melting, liquid storage tank is connected with each microchannel branch road in hybrid reaction layer by through hole, the downward-sloping microchannel main road connected respectively in hybrid reaction layer of each microchannel branch road, this microchannel main road is communicated with the reaction tank be positioned at bottom hybrid reaction layer vertically downward; And the height of each liquid storage tank is all higher than reaction tank, namely reaction tank is positioned at the bottom of micro-fluidic chip; The described reaction reagent that needs that described heating arrangement is suitable for being arranged in reaction tank is heated; Further, described hybrid reaction layer is also provided with a pipeline being suitable for microchannel main road to communicate with the external world, and this line portals is coated with sealing film; At medicament after completion of the reaction, tear sealing film, described sampler extracts reactant liquor out chemical examination by inserting needle tubing; Described sampler comprises the negative pressure cavity be connected with needle tubing, namely by needle tubing by reactant liquor this negative pressure cavity of suction from reaction tank.
Further, in described intermediate connecting layer, be inlaid with heater strip, and each heater strip is set around sealing place of corresponding paraffin respectively; The multi-channel output of described supply module is connected with the energization input of each heater strip respectively, and this supply module controls multiple-channel output by processor module; Control supply module by processor module to heat micro-fluidic chip, namely control heater strip electrified regulation by described processor module, to melt paraffin, liquid storage tank is communicated with reaction tank.
Further, described pressurizing unit adopts air curtain, and is provided with some gas orifices in air curtain, and each gas orifice is respectively to corresponding accurate liquid storage tank position; The steam line of each gas orifice is respectively equipped with air valve, and the control end of each air valve is all connected with processor module; Step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises: control corresponding air valve by described processor module and open, gas orifice aims at liquid storage tank position ejection gas, to oppress liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast; Or described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod is driven by corresponding screw body respectively, and the drive motor of each screw body controls by described processor module; Step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises: control respective drive motor by described processor module and rotate, aim at liquid storage tank position to be driven flexible extruded rod by screw body to stretch out, compressing liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
The invention has the beneficial effects as follows, (1), by vibrating mechanism and heating arrangement, improves the reaction efficiency of reagent, and in the micro-fluidic chip of sealing, can also carry out fast sampling to reactant liquor, improves conventional efficient; (2) by pre-packaged for reaction reagent in micro-fluidic chip, decrease operator's load procedure; (3) realizing reagent robotization by this multifunctional microflow control intelligence experimental provision moves in the reaction tank of hybrid reaction layer; (4) by pressurizing unit, its quick fluid-discharge can be impelled, improve conventional efficient; (5) described pressurizing unit matches with heating wire, can realize adding one by one or simultaneously in certain sequence of reagent, meets special biochemical reaction needs.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 shows the structural representation of multifunctional microflow control of the present invention intelligence experimental provision;
Fig. 2 shows the control principle block diagram of multifunctional microflow control of the present invention intelligence experimental provision;
Fig. 3 shows the structural representation of micro-fluidic chip of the present invention;
Fig. 4 shows the structural representation of intermediate connecting layer of the present invention;
Fig. 5 shows the structural representation of a flexible extruded rod.
In figure: micro-fluidic chip 1, draw-in groove 2, supply module 3, pressurizing unit 4, diaphragm seal 100, reserve liquid layer 110, intermediate connecting layer 120, hybrid reaction layer 130, liquid storage tank 111, heating arrangement 112, paraffin 121, heater strip 122, through hole 123, power supply interface 124, microchannel branch road 131, microchannel main road 132, reaction tank 133, pipeline 134, sealing film 135, air curtain 400, gas orifice 401, flexible extruded rod 402, feed screw nut 403, screw mandrel 404, drive motor 405.
Embodiment
In conjunction with the accompanying drawings, the present invention is further detailed explanation.These accompanying drawings are the schematic diagram of simplification, only basic structure of the present invention are described in a schematic way, and therefore it only shows the formation relevant with the present invention.
Embodiment 1
As shown in Figure 1, the invention provides a kind of multifunctional microflow control intelligence experimental provision, comprising: be suitable for the draw-in groove 2 that micro-fluidic chip 1 vertically inserts, described draw-in groove 2 is arranged on a vibrating mechanism 5, and described vibrating mechanism 5 is controlled by processor module; And in the groove of draw-in groove, being provided with the heating arrangement 112 controlled by described processor module, this heating arrangement 112 is suitable for heating micro-fluidic chip domestic demand reaction reagent.
The described reagent needing reaction reagent to refer to enter in micro-fluidic chip 1 in reaction tank 133.
Described heating arrangement 112 comprises heating plate, this heating plate is positioned at described groove, and contact with the bottom of micro-fluidic chip 1, this heating plate is controlled by driving power module by described processor module, preferably, the electric current being controlled driving power module by processor module is exported, and controls the temperature of heating plate to reach.
And described micro-fluidic intelligent experimental provision also comprises the sampler being suitable for sampling reactant liquor in micro-fluidic chip.
Described vibrating mechanism 5 comprises eccentric motor (vibrating motor), and eccentric motor is controlled by described processor module, is rotated vibrate, to improve reagent reacting efficiency in micro-fluidic chip 1 by eccentric motor.
Described multifunctional microflow control intelligence experimental provision also comprises: the supply module 3 heated for micro-fluidic chip 1, and is positioned at the pressurizing unit 4 of described draw-in groove 2 side; Described supply module 3, pressurizing unit 4 control by processor module, and described processor module is also connected with a temperature sensor, with after micro-fluidic chip 1 is heated to uniform temperature, control pressurizing unit 4 transverse shear stress micro-fluidic chip 1.
Concrete, described micro-fluidic chip 1 adopts multilayer vertically to arrange (adopting vertical), and comprises the reagent reserve liquid layer 110, intermediate connecting layer 120, the hybrid reaction layer 130 that are arranged in order; Distribute in described reagent reserve liquid layer 110 some liquid storage tanks 111, and each liquid storage tank 111 is sealed by the diaphragm seal 100 and intermediate connecting layer 120 being covered in agent reserve liquid layer 110 surface; Described intermediate connecting layer 120 is provided with the through hole 123 of liquid storage tank 111 discharge opeing, and through hole 123 is sealed (making through hole 123 be equivalent to a valve) by paraffin 121, when paraffin 121 melts (being equivalent to valve open), liquid storage tank 111 is connected with each microchannel branch road 131 in hybrid reaction layer 130 by through hole 123, the downward-sloping microchannel main road 132 connected respectively in hybrid reaction layer 130 of each microchannel branch road 131, this microchannel main road 132 is communicated with the reaction tank 133 be positioned at bottom hybrid reaction layer 130 vertically downward; And the height of each liquid storage tank 111 is all higher than reaction tank 133, namely described reaction tank is positioned at the bottom of micro-fluidic chip; Described hybrid reaction layer is also provided with a pipeline being suitable for microchannel main road to communicate with the external world, and this line portals is coated with sealing film, at medicament after completion of the reaction, tears sealing film, and described sampler extracts reactant liquor out chemical examination by inserting needle tubing; As sampler one preferred embodiment, described sampler comprises the negative pressure cavity be connected with needle tubing, by reactant liquor this negative pressure cavity of suction from reaction tank.Described negative pressure cavity can by air pump extracting air to be formed; Also can adopt known negative pressure technique to realize.Further, air pump is controlled by described processor module.
And the described reaction reagent that needs that described heating arrangement is suitable for being arranged in reaction tank is heated.
Described temperature sensor is connected near each valve by a miniature probe, to detect paraffin melting temperature.
Described hybrid reaction layer 130 is also provided with a pipeline 134 being suitable for microchannel main road 132 to communicate with the external world, this line portals is coated with sealing film 135, can in use, or after completion of the reaction, tear sealing film, insert needle tubing from described pipeline 134 and extract reactant liquor out chemical examination.
There is drop between described liquid storage tank 111 and reaction tank 133, the reagent being convenient to flow into reaction tank 133 can not pour in down a chimney in liquid storage tank 111.
Wherein, described diaphragm seal 100 adopts elastic film, and the base material of described reagent reserve liquid layer 110, intermediate connecting layer 120, hybrid reaction layer 130 is such as but not limited to plastic sheet, glass sheet, piezoid, PVC or silicon chip.
Further, in described intermediate connecting layer 120, heater strip 122 is inlaid with, and each heater strip 122 winding corresponding paraffin 121 sealing place respectively; The multi-channel output of described supply module 3 is connected with the energization input (i.e. power supply interface 124) of each heater strip 122 respectively, and this supply module 3 controls multiple-channel output by processor module; Heater strip 122 electrified regulation is controlled, to melt paraffin 121 by described processor module.
Paraffin dissolve after and water insoluble, be namely insoluble to reagent.
And because microchannel branch road 131 is wider, after paraffin melting, freeze solidly on its empty interior bottom, microchannel branch road 131 can not be blocked.
Wherein, connecting triode can be distinguished in each road of the multi-channel output of described supply module 3, and the control end of triode (base stage) is connected with each control end of processor module respectively, provide triode ON level by processor or turn off the control respectively that level realizes multi-channel output, and then selecting corresponding liquid storage tank 111 to open.
Preferably, described pressurizing unit 4 adopts air curtain 400, and is provided with some gas orifices 401 in air curtain 400, and each gas orifice 401 aims at liquid storage tank 111 position respectively; The steam line of each gas orifice 401 is respectively equipped with air valve, and the control end of each air valve is all connected with processor module, control corresponding air valve by described processor module to open, gas orifice 401 aims at liquid storage tank 111 position ejection gas, to oppress liquid storage tank 111, make after the paraffin 121 of this liquid storage tank 111 correspondence melts, the reagent in liquid storage tank 111 enters fast in reaction tank 133.
Because described diaphragm seal 100 adopts elastic film, so by extruding liquid storage tank 111, the reagent in pond can be made to discharge fast, improves reaction velocity and conventional efficient after paraffin 121 melts.
As shown in Figure 5, as another preferred embodiment of pressurizing unit 4, described pressurizing unit comprises some flexible extruded rods 402, and each flexible extruded rod 402 is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod 402 is driven by corresponding screw body respectively, namely stretches out compressing liquid storage tank, and make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast; The drive motor 405 of described each screw body controls by described processor module.
Fig. 5 illustrates the concrete structure of a flexible extruded rod 402, namely described flexible extruded rod adopts hollow structure, and feed screw nut 403 installs therein, described feed screw nut 403 coordinates with screw mandrel 404 and forms screw body, described screw mandrel 404 is by drive motor 405 driven rotary, to control the flexible of flexible extruded rod 402, and the head of described flexible extruded rod 402 can also adopt rubber structure, makes pressure more even.
Multifunctional microflow control of the present invention intelligence experimental provision, when testing, has reduced or remitted traditional load procedure, to be prestored reagent by the liquid storage tank 111 in micro-fluidic chip 1, and can to prestore different types of reagent according to the quantity of liquid storage tank 111.Coordinated with heater strip 122 by air curtain 400 or some flexible extruded rods 402, the reagent needed can be selected fast to join in reaction tank 133 test, also reagent addition sequence can be adjusted, namely by controlling heater strip 122 respectively and be energized and gas orifice 401 or corresponding flexible extruded rod 402 realizing; And after reagent enters into reaction tank 133, Vibration on Start-up mechanism 5 impels reagent in reaction tank 133 to react rapidly, improve reaction efficiency.
Described processor module is such as but not limited to employing 51 series monolithic.
Embodiment 2
On embodiment 1 basis, present invention also offers a kind of method of work of described multifunctional microflow control intelligence experimental provision, comprise the steps:
Step S1, heats micro-fluidic chip 1; And
Step S2, controls pressurizing unit 4 transverse shear stress micro-fluidic chip 1;
Step S3, Vibration on Start-up mechanism 5, carries out dither to micro-fluidic chip 1, and heats micro-fluidic chip domestic demand reaction reagent, makes its sufficient reacting; And
Step S4, is chemically examined by sampler abstraction reaction liquid from micro-fluidic chip.
Further, described multifunctional microflow control intelligence experimental provision comprises: the supply module 3 heated for micro-fluidic chip 1, and is positioned at the pressurizing unit 4 of described draw-in groove 2 side; Described supply module 3, pressurizing unit 4 control by processor module, and described processor module is also connected with a temperature sensor; In described step S1, the method that micro-fluidic chip 1 heats is comprised: control supply module 3 pairs of micro-fluidic chips 1 by processor module and heat.
And in the groove of draw-in groove, being provided with the heating arrangement 112 controlled by described processor module, this heating arrangement 112 is suitable for heating micro-fluidic chip domestic demand reaction reagent.
Described micro-fluidic chip 1 adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer 110, intermediate connecting layer 120, the hybrid reaction layer 130 that are arranged in order; Distribute in described reagent reserve liquid layer 110 some liquid storage tanks 111, and each liquid storage tank 111 is sealed by the diaphragm seal 100 and intermediate connecting layer 120 being covered in agent reserve liquid layer 110 surface; Described intermediate connecting layer 120 is provided with the through hole 123 of liquid storage tank 111 discharge opeing, and through hole 123 is sealed by paraffin 121, when paraffin 121 melts, liquid storage tank 111 is connected with each microchannel branch road 131 in hybrid reaction layer 130 by through hole 123, the downward-sloping microchannel main road 132 connected respectively in hybrid reaction layer 130 of each microchannel branch road 131, this microchannel main road 132 is communicated with the reaction tank 133 be positioned at bottom hybrid reaction layer 130 vertically downward; And the height of each liquid storage tank 111 is all higher than reaction tank 133, namely reaction tank is positioned at the bottom of micro-fluidic chip; The described reaction reagent that needs that described heating arrangement is suitable for being arranged in reaction tank is heated; Further, described hybrid reaction layer is also provided with a pipeline being suitable for microchannel main road to communicate with the external world, and this line portals is coated with sealing film; At medicament after completion of the reaction, tear sealing film, described sampler extracts reactant liquor out chemical examination by inserting needle tubing; Described sampler comprises the negative pressure cavity be connected with needle tubing, namely by needle tubing by reactant liquor this negative pressure cavity of suction from reaction tank.
Heater strip 122 is inlaid with in described intermediate connecting layer 120, and each heater strip 122 winding corresponding paraffin 121 sealing place respectively;
The multi-channel output of described supply module 3 is connected with the energization input of each heater strip 122 respectively, and this supply module 3 controls multiple-channel output by processor module; Control supply module 3 pairs of micro-fluidic chips 1 by processor module to heat, namely control heater strip 122 electrified regulation by described processor module, to melt paraffin 121, liquid storage tank 111 is communicated with reaction tank 133.
Described pressurizing unit 4 adopts air curtain 400, and is provided with some gas orifices 401 in air curtain 400, and each gas orifice 401 aims at liquid storage tank 111 position respectively; The steam line of each gas orifice 401 is respectively equipped with air valve, and the control end of each air valve is all connected with processor module; And step S2, the method controlling pressurizing unit 4 transverse shear stress micro-fluidic chip 1 comprises: control corresponding air valve by described processor module and open, gas orifice 401 aims at liquid storage tank 111 position ejection gas, to oppress liquid storage tank 111, make after the paraffin 121 of this liquid storage tank 111 correspondence melts, the reagent in liquid storage tank 111 enters fast in reaction tank 133.
And, further, by described processor module can make gas orifice 401 and liquid storage tank 111 relative to, realize each liquid storage tank 111 and control respectively or simultaneously, being specially adapted to store by the liquid storage tank 111 of different reagent when completing some experiment, needing to regulate and control each reagent to throw in speed.Concrete, can heat one by one heating wire, and gas orifice 401 is also according to the jet extruding one by one of corresponding order, completes the progressively input of reagent, meets experiment needs.
Optionally, described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod is driven by corresponding screw body respectively, and the drive motor of each screw body controls by described processor module;
On this basis, step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises: control respective drive motor by described processor module and rotate, aim at liquid storage tank position to be driven flexible extruded rod by screw body to stretch out, compressing liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
With above-mentioned according to desirable embodiment of the present invention for enlightenment, by above-mentioned description, relevant staff in the scope not departing from this invention technological thought, can carry out various change and amendment completely.The technical scope of this invention is not limited to the content on instructions, must determine its technical scope according to right.
Claims (10)
1. a multifunctional microflow control intelligence experimental provision, is characterized in that, comprising:
Be suitable for the draw-in groove that micro-fluidic chip vertically inserts, described draw-in groove is arranged on a vibrating mechanism, and described vibrating mechanism is controlled by processor module; And
In the groove of draw-in groove, be provided with the heating arrangement controlled by described processor module, this heating arrangement is suitable for heating micro-fluidic chip domestic demand reaction reagent; And
Described micro-fluidic intelligent experimental provision also comprises the sampler being suitable for sampling reactant liquor in micro-fluidic chip.
2. multifunctional microflow control intelligence experimental provision according to claim 1, is characterized in that,
Described multifunctional microflow control intelligence experimental provision also comprises: for the supply module of micro-fluidic chip heating, and be positioned at the pressurizing unit of described draw-in groove side;
Described supply module, pressurizing unit control by processor module, and described processor module is also connected with a temperature sensor, with after micro-fluidic chip is heated to uniform temperature, control pressurizing unit transverse shear stress micro-fluidic chip.
3. multifunctional microflow control intelligence experimental provision according to claim 2, is characterized in that,
Described micro-fluidic chip adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer, intermediate connecting layer, the hybrid reaction layer that are arranged in order;
Distribute in described reagent reserve liquid layer some liquid storage tanks, and each liquid storage tank is by being covered in diaphragm seal and the intermediate connecting layer sealing on agent reserve liquid layer surface;
Described intermediate connecting layer is provided with the through hole of liquid storage tank discharge opeing, and through hole is sealed by paraffin, when paraffin melting, liquid storage tank is connected with each microchannel branch road in hybrid reaction layer by through hole, the downward-sloping microchannel main road connected respectively in hybrid reaction layer of each microchannel branch road, this microchannel main road is communicated with the reaction tank be positioned at bottom hybrid reaction layer vertically downward; And
The height of each liquid storage tank is all higher than reaction tank, and namely reaction tank is positioned at the bottom of micro-fluidic chip;
The described reaction reagent that needs that described heating arrangement is suitable for being arranged in reaction tank is heated;
Further, described hybrid reaction layer is also provided with a pipeline being suitable for microchannel main road to communicate with the external world, and this line portals is coated with sealing film; At medicament after completion of the reaction, tear sealing film, described sampler extracts reactant liquor out chemical examination by inserting needle tubing; Described sampler comprises the negative pressure cavity be connected with needle tubing, namely by needle tubing by reactant liquor this negative pressure cavity of suction from reaction tank.
4. multifunctional microflow control intelligence experimental provision according to claim 3, is characterized in that,
Be inlaid with heater strip in described intermediate connecting layer, and each heater strip is set around sealing place of corresponding paraffin respectively;
The multi-channel output of described supply module is connected with the energization input of each heater strip respectively, and this supply module controls multiple-channel output by processor module;
Heater strip electrified regulation is controlled, to melt paraffin by described processor module.
5. multifunctional microflow control intelligence experimental provision according to claim 4, it is characterized in that, described pressurizing unit adopts air curtain, and is provided with some gas orifices in air curtain, and each gas orifice is respectively to corresponding accurate liquid storage tank position;
The steam line of each gas orifice is respectively equipped with air valve, and the control end of each air valve is all connected with processor module, control corresponding air valve by described processor module to open, gas orifice aims at liquid storage tank position ejection gas, to oppress liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
6. multifunctional microflow control intelligence experimental provision according to claim 4, it is characterized in that, described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position;
Described each flexible extruded rod is driven by corresponding screw body respectively, namely stretches out compressing liquid storage tank, and make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast;
The drive motor of described each screw body controls by described processor module.
7. a method of work for multifunctional microflow control intelligence experimental provision according to claim 1, is characterized in that, comprise the steps:
Step S1, heats micro-fluidic chip;
Step S2, controls pressurizing unit transverse shear stress micro-fluidic chip;
Step S3, Vibration on Start-up mechanism, carries out dither to micro-fluidic chip, and heats micro-fluidic chip domestic demand reaction reagent, makes its sufficient reacting; And
Step S4, is chemically examined by sampler abstraction reaction liquid from micro-fluidic chip.
8. the method for work of multifunctional microflow control intelligence experimental provision according to claim 7, is characterized in that,
Described multifunctional microflow control intelligence experimental provision comprises: for the supply module of micro-fluidic chip heating, and be positioned at the pressurizing unit of described draw-in groove side;
Described supply module, pressurizing unit control by processor module, and described processor module is also connected with a temperature sensor;
In described step S1, the method for micro-fluidic chip heating is comprised:
Control supply module by processor module to heat micro-fluidic chip.
9. the method for work of multifunctional microflow control intelligence experimental provision according to claim 8, is characterized in that,
Described micro-fluidic chip adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer, intermediate connecting layer, the hybrid reaction layer that are arranged in order;
Distribute in described reagent reserve liquid layer some liquid storage tanks, and each liquid storage tank is by being covered in diaphragm seal and the intermediate connecting layer sealing on agent reserve liquid layer surface;
Described intermediate connecting layer is provided with the through hole of liquid storage tank discharge opeing, and through hole is sealed by paraffin, when paraffin melting, liquid storage tank is connected with each microchannel branch road in hybrid reaction layer by through hole, the downward-sloping microchannel main road connected respectively in hybrid reaction layer of each microchannel branch road, this microchannel main road is communicated with the reaction tank be positioned at bottom hybrid reaction layer vertically downward; And
The height of each liquid storage tank is all higher than reaction tank, and namely reaction tank is positioned at the bottom of micro-fluidic chip;
The described reaction reagent that needs that described heating arrangement is suitable for being arranged in reaction tank is heated;
Further, described hybrid reaction layer is also provided with a pipeline being suitable for microchannel main road to communicate with the external world, and this line portals is coated with sealing film; At medicament after completion of the reaction, tear sealing film, described sampler extracts reactant liquor out chemical examination by inserting needle tubing; Described sampler comprises the negative pressure cavity be connected with needle tubing, namely by needle tubing by reactant liquor this negative pressure cavity of suction from reaction tank;
Be inlaid with heater strip in described intermediate connecting layer, and each heater strip is set around sealing place of corresponding paraffin respectively;
The multi-channel output of described supply module is connected with the energization input of each heater strip respectively, and this supply module controls multiple-channel output by processor module;
Control supply module by processor module to heat micro-fluidic chip, namely control heater strip electrified regulation by described processor module, to melt paraffin, liquid storage tank is communicated with reaction tank.
10. the method for work of multifunctional microflow control intelligence experimental provision according to claim 9, is characterized in that,
Described pressurizing unit adopts air curtain, and is provided with some gas orifices in air curtain, and each gas orifice is respectively to corresponding accurate liquid storage tank position; The steam line of each gas orifice is respectively equipped with air valve, and the control end of each air valve is all connected with processor module;
Step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises:
Control corresponding air valve by described processor module to open, gas orifice aims at liquid storage tank position ejection gas, and to oppress liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast; Or
Described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod is driven by corresponding screw body respectively, and the drive motor of each screw body controls by described processor module;
Step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises:
Control respective drive motor by described processor module to rotate, aim at liquid storage tank position to be driven flexible extruded rod by screw body to stretch out, compressing liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
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