CN105498654A - Micro-fluidic intelligent experimental device with temperature control function and working method thereof - Google Patents
Micro-fluidic intelligent experimental device with temperature control function and working method thereof Download PDFInfo
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- CN105498654A CN105498654A CN201510869773.8A CN201510869773A CN105498654A CN 105498654 A CN105498654 A CN 105498654A CN 201510869773 A CN201510869773 A CN 201510869773A CN 105498654 A CN105498654 A CN 105498654A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0006—Controlling or regulating processes
- B01J19/0013—Controlling the temperature of the process
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00002—Chemical plants
- B01J2219/00004—Scale aspects
- B01J2219/00011—Laboratory-scale plants
- B01J2219/00013—Miniplants
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00049—Controlling or regulating processes
- B01J2219/00051—Controlling the temperature
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00049—Controlling or regulating processes
- B01J2219/00051—Controlling the temperature
- B01J2219/00132—Controlling the temperature using electric heating or cooling elements
- B01J2219/00135—Electric resistance heaters
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00049—Controlling or regulating processes
- B01J2219/00162—Controlling or regulating processes controlling the pressure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
- B01J2219/00786—Geometry of the plates
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00873—Heat exchange
- B01J2219/00882—Electromagnetic heating
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00891—Feeding or evacuation
- B01J2219/00896—Changing inlet or outlet cross-section, e.g. pressure-drop compensation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/0095—Control aspects
- B01J2219/00952—Sensing operations
- B01J2219/00954—Measured properties
- B01J2219/00961—Temperature
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/0095—Control aspects
- B01J2219/00952—Sensing operations
- B01J2219/00954—Measured properties
- B01J2219/00963—Pressure
Abstract
The invention relates to a micro-fluidic intelligent experimental device with a temperature control function and a working method thereof. The micro-fluidic intelligent experimental device comprises a clamping groove suitable for a micro-fluidic chip to be vertically inserted therein and a heating device arranged in the clamping groove and controlled by a processor module, wherein the clamping groove is mounted on a vibration mechanism, and the vibration mechanism is controlled by the processor module; the heating device is suitable for heating up required reaction reagents in the micro-fluidic chip. By virtue of the vibration mechanism and the heating device, the reagent reaction efficiency is improved; as the reaction reagents are pre-packaged in the micro-fluidic chip, a sampling step is omitted for an operator; the intelligent experimental device can realize automatic movement of the reagents into a reaction tank at the bottom of a hybrid reaction layer; quick liquor drainage can be promoted by a squeezing device, so that the experimental efficiency is improved; as the squeezing device is matched with heater strips, the reagents can be added one by one in order or added simultaneously to meet the demand for special biochemical reaction.
Description
Technical field
The present invention relates to a kind of microfluidic experimental device, particularly relate to a kind of with temperature controlled micro-fluidic intelligent experimental provision and method of work thereof.
Background technology
Biochemistry detection micro-fluidic chip is multifunction system chip, this chip is formed in integrated for the basic operation units such as the sample preparation involved by biochemistry detection, quantitative sample injection, liquid mixing, biochemical reaction, separation detection or baseset on the chip of several square centimeters, is a kind of technology platform of the various functions replacing conventional chemical or biology laboratory.
How to make the reagent fast reaction in micro-fluidic chip, improve the technical barrier that testing efficiency is this area.
Summary of the invention
The object of this invention is to provide a kind of with temperature controlled micro-fluidic intelligent experimental provision and method of work thereof, micro-fluidic chip domestic demand reaction reagent is impelled to add fast response by vibrating mechanism and heater, to solve the inefficient technical problem of reagent reacting in micro-fluidic chip.
In order to solve the problems of the technologies described above, the invention provides a kind of with temperature controlled micro-fluidic intelligent experimental provision, be suitable for the draw-in groove that micro-fluidic chip vertically inserts, described draw-in groove is arranged on a vibrating mechanism, and described vibrating mechanism is controlled by processor module; And in the groove of draw-in groove, being provided with the heater controlled by described processor module, this heater is suitable for heating micro-fluidic chip domestic demand reaction reagent.
Further, described micro-fluidic intelligent experimental provision also comprises: be suitable for the draw-in groove that micro-fluidic chip vertically inserts, and for the supply module of micro-fluidic chip heating, and is positioned at the pressurizing unit of described draw-in groove side; Described supply module, pressurizing unit control by processor module, and described processor module is also connected with a temperature sensor, with after micro-fluidic chip is heated to uniform temperature, control pressurizing unit transverse shear stress micro-fluidic chip.
Further, described micro-fluidic chip adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer, intermediate connecting layer, the hybrid reaction layer that are arranged in order; Distribute in described reagent reserve liquid layer some liquid storage tanks, and each liquid storage tank is by being covered in diaphragm seal and the intermediate connecting layer sealing on agent reserve liquid layer surface; Described intermediate connecting layer is provided with the through hole of liquid storage tank discharge opeing, and through hole is sealed by paraffin, when paraffin melting, liquid storage tank is connected with each microchannel branch road in hybrid reaction layer by through hole, the downward-sloping microchannel main road connected respectively in hybrid reaction layer of each microchannel branch road, this microchannel main road is communicated with the reaction tank be positioned at bottom hybrid reaction layer vertically downward; And the height of each liquid storage tank is all higher than reaction tank, namely reaction tank is positioned at the bottom of micro-fluidic chip; The described reaction reagent that needs that described heater is suitable for being arranged in reaction tank is heated.
Further, in described intermediate connecting layer, be inlaid with heater strip, and each heater strip is set around sealing place of corresponding paraffin respectively; The multi-channel output of described supply module is connected with the energization input of each heater strip respectively, and this supply module controls multiple-channel output by processor module; Heater strip electrified regulation is controlled, to melt paraffin by described processor module.
Further, described pressurizing unit adopts air curtain, and is provided with some fumaroles in air curtain, and each fumarole is respectively to corresponding accurate liquid storage tank position; The steam line of each fumarole is respectively equipped with air valve, and the control end of each air valve is all connected with processor module, control corresponding air valve by described processor module to open, fumarole aims at liquid storage tank position ejection gas, to oppress liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
Further, described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod is driven by corresponding screw body respectively, namely stretches out compressing liquid storage tank, and make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast; And the drive motors of each screw body controls by described processor module.
Another aspect, present invention also offers a kind of method of work of described micro-fluidic intelligent experimental provision, comprises the steps:
Step S1, heats micro-fluidic chip;
Step S2, controls pressurizing unit transverse shear stress micro-fluidic chip;
Step S3, Vibration on Start-up mechanism, carries out dither to micro-fluidic chip, and heats micro-fluidic chip domestic demand reaction reagent, makes its sufficient reacting.
Further, described micro-fluidic intelligent experimental provision comprises: for the supply module of micro-fluidic chip heating, and be positioned at the pressurizing unit of described draw-in groove side; Described supply module, pressurizing unit control by processor module, and described processor module is also connected with a temperature sensor; In described step S1, the method for micro-fluidic chip heating is comprised: control supply module by processor module and micro-fluidic chip is heated.
Further, described micro-fluidic chip adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer, intermediate connecting layer, the hybrid reaction layer that are arranged in order; Distribute in described reagent reserve liquid layer some liquid storage tanks, and each liquid storage tank is by being covered in diaphragm seal and the intermediate connecting layer sealing on agent reserve liquid layer surface; Described intermediate connecting layer is provided with the through hole of liquid storage tank discharge opeing, and through hole is sealed by paraffin, when paraffin melting, liquid storage tank is connected with each microchannel branch road in hybrid reaction layer by through hole, the downward-sloping microchannel main road connected respectively in hybrid reaction layer of each microchannel branch road, this microchannel main road is communicated with the reaction tank be positioned at bottom hybrid reaction layer vertically downward; And the height of each liquid storage tank is all higher than reaction tank.
Further, in described intermediate connecting layer, be inlaid with heater strip, and each heater strip is set around sealing place of corresponding paraffin respectively; The multi-channel output of described supply module is connected with the energization input of each heater strip respectively, and this supply module controls multiple-channel output by processor module; Control supply module by processor module to heat micro-fluidic chip, namely control heater strip electrified regulation by described processor module, to melt paraffin, liquid storage tank is communicated with reaction tank.
Further, described pressurizing unit adopts air curtain, and is provided with some fumaroles in air curtain, and each fumarole is respectively to corresponding accurate liquid storage tank position; The steam line of each fumarole is respectively equipped with air valve, and the control end of each air valve is all connected with processor module; Step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises: control corresponding air valve by described processor module and open, fumarole aims at liquid storage tank position ejection gas, to oppress liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast; Or described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod is driven by corresponding screw body respectively, and the drive motors of each screw body controls by described processor module; Step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises: control respective drive motor by described processor module and rotate, aim at liquid storage tank position to be driven flexible extruded rod by screw body to stretch out, compressing liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
The invention has the beneficial effects as follows, (1), by vibrating mechanism and heater, improves the reaction efficiency of reagent; (2) by pre-packaged for reaction reagent in micro-fluidic chip, decrease operator's load procedure; (3) realizing reagent automation by this intelligent experimental provision moves in the reaction tank of hybrid reaction layer; (4) by pressurizing unit, its quick fluid-discharge can be impelled, improve conventional efficient; (5) described pressurizing unit matches with heating wire, can realize adding one by one or simultaneously in certain sequence of reagent, meets special biochemical reaction needs.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 shows the structural representation of micro-fluidic intelligent experimental provision of the present invention;
Fig. 2 shows the control principle block diagram of micro-fluidic intelligent experimental provision of the present invention;
Fig. 3 shows the structural representation of micro-fluidic chip of the present invention;
Fig. 4 shows the structural representation of intermediate connecting layer of the present invention;
Fig. 5 shows the structural representation of a flexible extruded rod.
In figure: micro-fluidic chip 1, draw-in groove 2, supply module 3, pressurizing unit 4, diaphragm seal 100, reserve liquid layer 110, intermediate connecting layer 120, hybrid reaction layer 130, liquid storage tank 111, heater 112, paraffin 121, heater strip 122, through hole 123, power supply interface 124, microchannel branch road 131, microchannel main road 132, reaction tank 133, pipeline 134, sealing film 135, air curtain 400, fumarole 401, flexible extruded rod 402, feed screw nut 403, screw mandrel 404, drive motors 405.
Detailed description of the invention
In conjunction with the accompanying drawings, the present invention is further detailed explanation.These accompanying drawings are the schematic diagram of simplification, only basic structure of the present invention are described in a schematic way, and therefore it only shows the formation relevant with the present invention.
Embodiment 1
As shown in Figure 1, the invention provides a kind of with temperature controlled micro-fluidic intelligent experimental provision, comprising: be suitable for the draw-in groove 2 that micro-fluidic chip 1 vertically inserts, described draw-in groove 2 is arranged on a vibrating mechanism 5, and described vibrating mechanism 5 is controlled by processor module; And in the groove of draw-in groove, being provided with the heater 112 controlled by described processor module, this heater 112 is suitable for heating micro-fluidic chip domestic demand reaction reagent.
The described reagent needing reaction reagent to refer to enter in micro-fluidic chip 1 in reaction tank 133.
Described heater 112 comprises heating plate, this heating plate is positioned at described groove, and contact with the bottom of micro-fluidic chip 1, this heating plate is controlled by driving power module by described processor module, preferably, the electric current being controlled driving power module by processor module is exported, and controls the temperature of heating plate to reach.
Described vibrating mechanism 5 comprises eccentric motor (vibrating motor), and eccentric motor is controlled by described processor module, is rotated vibrate, to improve reagent reacting efficiency in micro-fluidic chip 1 by eccentric motor.
This micro-fluidic intelligent experimental provision collaborative work by heating, vibrating, to improve the reaction speed of reagent, and can meet the reaction needed of particular agent.
Described micro-fluidic intelligent experimental provision also comprises: the supply module 3 heated for micro-fluidic chip 1, and is positioned at the pressurizing unit 4 of described draw-in groove 2 side; Described supply module 3, pressurizing unit 4 control by processor module, and described processor module is also connected with a temperature sensor, with after micro-fluidic chip 1 is heated to uniform temperature, control pressurizing unit 4 transverse shear stress micro-fluidic chip 1.
Concrete, described micro-fluidic chip 1 adopts multilayer vertically to arrange (adopting vertical), and comprises the reagent reserve liquid layer 110, intermediate connecting layer 120, the hybrid reaction layer 130 that are arranged in order; Distribute in described reagent reserve liquid layer 110 some liquid storage tanks 111, and each liquid storage tank 111 is sealed by the diaphragm seal 100 and intermediate connecting layer 120 being covered in agent reserve liquid layer 110 surface; Described intermediate connecting layer 120 is provided with the through hole 123 of liquid storage tank 111 discharge opeing, and through hole 123 is sealed (making through hole 123 be equivalent to a valve) by paraffin 121, when paraffin 121 melts (being equivalent to valve open), liquid storage tank 111 is connected with each microchannel branch road 131 in hybrid reaction layer 130 by through hole 123, the downward-sloping microchannel main road 132 connected respectively in hybrid reaction layer 130 of each microchannel branch road 131, this microchannel main road 132 is communicated with the reaction tank 133 be positioned at bottom hybrid reaction layer 130 vertically downward; And the height of each liquid storage tank 111 is all higher than reaction tank 133, namely reaction tank is positioned at the bottom of micro-fluidic chip; The described reaction reagent that needs that described heater is suitable for being arranged in reaction tank is heated.
Described temperature sensor is connected near each valve by a miniature probe, to detect paraffin melting temperature.
Described hybrid reaction layer 130 is also provided with a pipeline 134 being suitable for microchannel main road 132 to communicate with the external world, this line portals is coated with sealing film 135, can in use, or after completion of the reaction, tear sealing film, insert needle tubing from described pipeline 134 and extract reactant liquor out chemical examination.
There is drop between described liquid storage tank 111 and reaction tank 133, the reagent being convenient to flow into reaction tank 133 can not pour in down a chimney in liquid storage tank 111.
Wherein, described diaphragm seal 100 adopts elastic film, and the base material of described reagent reserve liquid layer 110, intermediate connecting layer 120, hybrid reaction layer 130 is such as but not limited to plastic sheet, sheet glass, quartz plate, PVC or silicon chip.
Further, in described intermediate connecting layer 120, heater strip 122 is inlaid with, and each heater strip 122 winding corresponding paraffin 121 sealing place respectively; The multi-channel output of described supply module 3 is connected with the energization input (i.e. power supply interface 124) of each heater strip 122 respectively, and this supply module 3 controls multiple-channel output by processor module; Heater strip 122 electrified regulation is controlled, to melt paraffin 121 by described processor module.
Paraffin dissolve after and water insoluble, be namely insoluble to reagent.
And because microchannel branch road 131 is wider, after paraffin melting, freeze solidly on its empty interior bottom, microchannel branch road 131 can not be blocked.
Wherein, connecting triode can be distinguished in each road of the multi-channel output of described supply module 3, and the control end of triode (base stage) is connected with each control end of processor module respectively, provide triode ON level by processor or turn off the control respectively that level realizes multi-channel output, and then selecting corresponding liquid storage tank 111 to open.
Preferably, described pressurizing unit 4 adopts air curtain 400, and is provided with some fumaroles 401 in air curtain 400, and each fumarole 401 aims at liquid storage tank 111 position respectively; The steam line of each fumarole 401 is respectively equipped with air valve, and the control end of each air valve is all connected with processor module, control corresponding air valve by described processor module to open, fumarole 401 aims at liquid storage tank 111 position ejection gas, to oppress liquid storage tank 111, make after the paraffin 121 of this liquid storage tank 111 correspondence melts, the reagent in liquid storage tank 111 enters fast in reaction tank 133.
Because described diaphragm seal 100 adopts elastic film, so by extruding liquid storage tank 111, the reagent in pond can be made to discharge fast, improves reaction speed and conventional efficient after paraffin 121 melts.
As shown in Figure 5, as another preferred embodiment of pressurizing unit 4, described pressurizing unit comprises some flexible extruded rods 402, and each flexible extruded rod 402 is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod 402 is driven by corresponding screw body respectively, namely stretches out compressing liquid storage tank, and make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast; And the drive motors 405 of each screw body controls by described processor module.
Fig. 5 illustrates the concrete structure of a flexible extruded rod 402, namely described flexible extruded rod adopts hollow structure, and feed screw nut 403 installs therein, described feed screw nut 403 coordinates with screw mandrel 404 and forms screw body, described screw mandrel 404 is by drive motors 405 driven rotary, to control the flexible of flexible extruded rod 402, and the head of described flexible extruded rod 402 can also adopt rubber structure, makes pressure more even.
Micro-fluidic intelligent experimental provision of the present invention, when testing, has reduced or remitted traditional load procedure, to be prestored reagent by the liquid storage tank 111 in micro-fluidic chip 1, and can to prestore different types of reagent according to the quantity of liquid storage tank 111.Coordinated with heater strip 122 by air curtain 400 or some flexible extruded rods 402, the reagent needed can be selected fast to join in reaction tank 133 test, also reagent addition sequence can be adjusted, namely by controlling heater strip 122 respectively and be energized and fumarole 401 or corresponding flexible extruded rod 402 realizing; And after reagent enters into reaction tank 133, Vibration on Start-up mechanism 5 impels reagent in reaction tank 133 to react rapidly, improve reaction efficiency.
Described processor module is such as but not limited to employing 51 series monolithic.
Embodiment 2
On embodiment 1 basis, present invention also offers a kind of method of work of described micro-fluidic intelligent experimental provision, comprise the steps:
Step S1, heats micro-fluidic chip 1; And
Step S2, controls pressurizing unit 4 transverse shear stress micro-fluidic chip 1;
Step S3, Vibration on Start-up mechanism 5, carries out dither to micro-fluidic chip, and heats micro-fluidic chip 1 domestic demand reaction reagent, makes its sufficient reacting.
Further, described micro-fluidic intelligent experimental provision comprises: the supply module 3 heated for micro-fluidic chip 1, and is positioned at the pressurizing unit 4 of described draw-in groove 2 side; Described supply module 3, pressurizing unit 4 control by processor module, and described processor module is also connected with a temperature sensor; In described step S1, the method that micro-fluidic chip 1 heats is comprised: control supply module 3 pairs of micro-fluidic chips 1 by processor module and heat.
Described micro-fluidic chip 1 adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer 110, intermediate connecting layer 120, the hybrid reaction layer 130 that are arranged in order; Distribute in described reagent reserve liquid layer 110 some liquid storage tanks 111, and each liquid storage tank 111 is sealed by the diaphragm seal 100 and intermediate connecting layer 120 being covered in agent reserve liquid layer 110 surface; Described intermediate connecting layer 120 is provided with the through hole 123 of liquid storage tank 111 discharge opeing, and through hole 123 is sealed by paraffin 121, when paraffin 121 melts, liquid storage tank 111 is connected with each microchannel branch road 131 in hybrid reaction layer 130 by through hole 123, the downward-sloping microchannel main road 132 connected respectively in hybrid reaction layer 130 of each microchannel branch road 131, this microchannel main road 132 is communicated with the reaction tank 133 be positioned at bottom hybrid reaction layer 130 vertically downward; And the height of each liquid storage tank 111 is all higher than reaction tank 133.
Heater strip 122 is inlaid with in described intermediate connecting layer 120, and each heater strip 122 winding corresponding paraffin 121 sealing place respectively;
The multi-channel output of described supply module 3 is connected with the energization input of each heater strip 122 respectively, and this supply module 3 controls multiple-channel output by processor module; Control supply module 3 pairs of micro-fluidic chips 1 by processor module to heat, namely control heater strip 122 electrified regulation by described processor module, to melt paraffin 121, liquid storage tank 111 is communicated with reaction tank 133.
Described pressurizing unit 4 adopts air curtain 400, and is provided with some fumaroles 401 in air curtain 400, and each fumarole 401 aims at liquid storage tank 111 position respectively; The steam line of each fumarole 401 is respectively equipped with air valve, and the control end of each air valve is all connected with processor module; And step S2, the method controlling pressurizing unit 4 transverse shear stress micro-fluidic chip 1 comprises: control corresponding air valve by described processor module and open, fumarole 401 aims at liquid storage tank 111 position ejection gas, to oppress liquid storage tank 111, make after the paraffin 121 of this liquid storage tank 111 correspondence melts, the reagent in liquid storage tank 111 enters fast in reaction tank 133.
And, further, by described processor module can make fumarole 401 and liquid storage tank 111 relative to, realize each liquid storage tank 111 and control respectively or simultaneously, being specially adapted to store by the liquid storage tank 111 of different reagent when completing some experiment, needing to regulate and control each reagent to throw in speed.Concrete, can heat one by one heating wire, and fumarole 401 is also according to the jet extruding one by one of corresponding order, completes the progressively input of reagent, meets experiment needs.
Optionally, described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod is driven by corresponding screw body respectively, and the drive motors of each screw body controls by described processor module;
On this basis, step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises: control respective drive motor by described processor module and rotate, aim at liquid storage tank position to be driven flexible extruded rod by screw body to stretch out, compressing liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
With above-mentioned according to desirable embodiment of the present invention for enlightenment, by above-mentioned description, relevant staff in the scope not departing from this invention technological thought, can carry out various change and amendment completely.The technical scope of this invention is not limited to the content on description, must determine its technical scope according to right.
Claims (10)
1., with a temperature controlled micro-fluidic intelligent experimental provision, it is characterized in that, comprising:
Be suitable for the draw-in groove that micro-fluidic chip vertically inserts, described draw-in groove is arranged on a vibrating mechanism, and described vibrating mechanism is controlled by processor module; And
In the groove of draw-in groove, be provided with the heater controlled by described processor module, this heater is suitable for heating micro-fluidic chip domestic demand reaction reagent.
2. micro-fluidic intelligent experimental provision according to claim 1, is characterized in that,
Described micro-fluidic intelligent experimental provision also comprises: for the supply module of micro-fluidic chip heating, and be positioned at the pressurizing unit of described draw-in groove side;
Described supply module, pressurizing unit control by processor module, and described processor module is also connected with a temperature sensor, with after micro-fluidic chip is heated to uniform temperature, control pressurizing unit transverse shear stress micro-fluidic chip.
3. micro-fluidic intelligent experimental provision according to claim 2, is characterized in that,
Described micro-fluidic chip adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer, intermediate connecting layer, the hybrid reaction layer that are arranged in order;
Distribute in described reagent reserve liquid layer some liquid storage tanks, and each liquid storage tank is by being covered in diaphragm seal and the intermediate connecting layer sealing on agent reserve liquid layer surface;
Described intermediate connecting layer is provided with the through hole of liquid storage tank discharge opeing, and through hole is sealed by paraffin, when paraffin melting, liquid storage tank is connected with each microchannel branch road in hybrid reaction layer by through hole, the downward-sloping microchannel main road connected respectively in hybrid reaction layer of each microchannel branch road, this microchannel main road is communicated with the reaction tank be positioned at bottom hybrid reaction layer vertically downward; And
The height of each liquid storage tank is all higher than reaction tank, and namely reaction tank is positioned at the bottom of micro-fluidic chip;
The described reaction reagent that needs that described heater is suitable for being arranged in reaction tank is heated.
4. micro-fluidic intelligent experimental provision according to claim 3, is characterized in that,
Be inlaid with heater strip in described intermediate connecting layer, and each heater strip is set around sealing place of corresponding paraffin respectively;
The multi-channel output of described supply module is connected with the energization input of each heater strip respectively, and this supply module controls multiple-channel output by processor module;
Heater strip electrified regulation is controlled, to melt paraffin by described processor module.
5. micro-fluidic intelligent experimental provision according to claim 4, is characterized in that, described pressurizing unit adopts air curtain, and is provided with some fumaroles in air curtain, and each fumarole is respectively to corresponding accurate liquid storage tank position;
The steam line of each fumarole is respectively equipped with air valve, and the control end of each air valve is all connected with processor module, control corresponding air valve by described processor module to open, fumarole aims at liquid storage tank position ejection gas, to oppress liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
6. micro-fluidic intelligent experimental provision according to claim 4, is characterized in that, described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position;
Described each flexible extruded rod is driven by corresponding screw body respectively, namely stretches out compressing liquid storage tank, and make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast;
The drive motors of described each screw body controls by described processor module.
7. a method of work for micro-fluidic intelligent experimental provision according to claim 1, is characterized in that, comprise the steps:
Step S1, heats micro-fluidic chip;
Step S2, controls pressurizing unit transverse shear stress micro-fluidic chip;
Step S3, Vibration on Start-up mechanism, carries out dither to micro-fluidic chip, and heats micro-fluidic chip domestic demand reaction reagent, makes its sufficient reacting.
8. the method for work of micro-fluidic intelligent experimental provision according to claim 7, is characterized in that,
Described micro-fluidic intelligent experimental provision comprises: for the supply module of micro-fluidic chip heating, and be positioned at the pressurizing unit of described draw-in groove side;
Described supply module, pressurizing unit control by processor module, and described processor module is also connected with a temperature sensor;
In described step S1, the method for micro-fluidic chip heating is comprised:
Control supply module by processor module to heat micro-fluidic chip.
9. the method for work of micro-fluidic intelligent experimental provision according to claim 8, is characterized in that,
Described micro-fluidic chip adopts multilayer vertically to arrange, and comprises the reagent reserve liquid layer, intermediate connecting layer, the hybrid reaction layer that are arranged in order;
Distribute in described reagent reserve liquid layer some liquid storage tanks, and each liquid storage tank is by being covered in diaphragm seal and the intermediate connecting layer sealing on agent reserve liquid layer surface;
Described intermediate connecting layer is provided with the through hole of liquid storage tank discharge opeing, and through hole is sealed by paraffin, when paraffin melting, liquid storage tank is connected with each microchannel branch road in hybrid reaction layer by through hole, the downward-sloping microchannel main road connected respectively in hybrid reaction layer of each microchannel branch road, this microchannel main road is communicated with the reaction tank be positioned at bottom hybrid reaction layer vertically downward; And
The height of each liquid storage tank is all higher than reaction tank;
Be inlaid with heater strip in described intermediate connecting layer, and each heater strip is set around sealing place of corresponding paraffin respectively;
The multi-channel output of described supply module is connected with the energization input of each heater strip respectively, and this supply module controls multiple-channel output by processor module;
Control supply module by processor module to heat micro-fluidic chip, namely control heater strip electrified regulation by described processor module, to melt paraffin, liquid storage tank is communicated with reaction tank.
10. the method for work of micro-fluidic intelligent experimental provision according to claim 9, is characterized in that,
Described pressurizing unit adopts air curtain, and is provided with some fumaroles in air curtain, and each fumarole is respectively to corresponding accurate liquid storage tank position; The steam line of each fumarole is respectively equipped with air valve, and the control end of each air valve is all connected with processor module;
Step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises:
Control corresponding air valve by described processor module to open, fumarole aims at liquid storage tank position ejection gas, and to oppress liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters in reaction tank fast; Or
Described pressurizing unit comprises some flexible extruded rods, and each flexible extruded rod is respectively to corresponding accurate liquid storage tank position; Described each flexible extruded rod is driven by corresponding screw body respectively, and the drive motors of each screw body controls by described processor module;
Step S2, the method controlling pressurizing unit transverse shear stress micro-fluidic chip comprises:
Control respective drive motor by described processor module to rotate, aim at liquid storage tank position to be driven flexible extruded rod by screw body to stretch out, compressing liquid storage tank, make after corresponding paraffin melting of this liquid storage tank, the reagent in liquid storage tank enters fast in reaction tank.
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