CN105543318A - Producing method of high-purity and high-dissolubility hydrolyzed rice protein - Google Patents
Producing method of high-purity and high-dissolubility hydrolyzed rice protein Download PDFInfo
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 103
- 235000009566 rice Nutrition 0.000 title claims abstract description 103
- 238000000034 method Methods 0.000 title claims abstract description 41
- 102000004169 proteins and genes Human genes 0.000 title abstract description 42
- 108090000623 proteins and genes Proteins 0.000 title abstract description 42
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 102
- 102000004190 Enzymes Human genes 0.000 claims abstract description 61
- 108090000790 Enzymes Proteins 0.000 claims abstract description 61
- 230000007062 hydrolysis Effects 0.000 claims abstract description 44
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 44
- 150000001875 compounds Chemical class 0.000 claims abstract description 23
- 229940088598 enzyme Drugs 0.000 claims description 59
- 239000000243 solution Substances 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 17
- 239000002893 slag Substances 0.000 claims description 17
- 238000002360 preparation method Methods 0.000 claims description 15
- 102000004139 alpha-Amylases Human genes 0.000 claims description 14
- 108090000637 alpha-Amylases Proteins 0.000 claims description 14
- 229940024171 alpha-amylase Drugs 0.000 claims description 14
- 238000005507 spraying Methods 0.000 claims description 14
- 108091005804 Peptidases Proteins 0.000 claims description 13
- 239000004365 Protease Substances 0.000 claims description 13
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 13
- 238000005406 washing Methods 0.000 claims description 13
- 239000011259 mixed solution Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 9
- 238000012545 processing Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 238000002203 pretreatment Methods 0.000 claims description 7
- 238000001694 spray drying Methods 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000006210 lotion Substances 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 230000002459 sustained effect Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 2
- 230000002457 bidirectional effect Effects 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 36
- 239000000047 product Substances 0.000 description 26
- 239000003513 alkali Substances 0.000 description 11
- 238000010792 warming Methods 0.000 description 10
- 239000003531 protein hydrolysate Substances 0.000 description 9
- 108010009736 Protein Hydrolysates Proteins 0.000 description 8
- 239000012141 concentrate Substances 0.000 description 6
- 235000008504 concentrate Nutrition 0.000 description 6
- 238000013016 damping Methods 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 230000001804 emulsifying effect Effects 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 229920001353 Dextrin Polymers 0.000 description 2
- 239000004375 Dextrin Substances 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 235000008452 baby food Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000019425 dextrin Nutrition 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 206010003645 Atopy Diseases 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 206010016946 Food allergy Diseases 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 108010073032 Grain Proteins Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
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- 239000006227 byproduct Substances 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- -1 compound compound Chemical class 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 206010016766 flatulence Diseases 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 230000000774 hypoallergenic effect Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 108010024951 plastein Proteins 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses a producing method of high-purity and high-dissolubility hydrolyzed rice protein. The high-purity and high-dissolubility hydrolyzed rice protein is obtained after bidirectional compound enzyme enzymolysis of rice dreg as well as decoloration and debittering. The purity of the hydrolyzed rice protein prepared with the method reaches 94% according to a test; the degree of hydrolysis of the product protein reaches 75%, the solubility reaches 98%, the average molecular weight is 830 Da, the molecular weight of 85% of the protein is concentrated on 790 Da, the emulsibility and the foamability of the product are greatly improved, and the producing method has a good application prospect.
Description
Technical field
The present invention relates to a kind of production method of rice protein, particularly enzymatic hydrolysis rice protein, obtain the production method of the soluble rice protein of high purity.
Background technology
Biological value (B.V.) and the protein value (P.V.) of rice protein all rank first in various grain protein, protein in rice it contain whole amino acid of needed by human body, have high nutrition, hypoallergenic, local flavor gentle, can not the peculiar properties such as flatulence be caused.Rice is again uniquely can in order to avoid the cereal of allergic experiment, the clinical study of the U.S. shows, hyperirritability venereal disease people only less than 1% in about 700 atopic cases can be irritated to rice protein, and paediatrics also rarely has the case of report to rice protein allergy.So rice protein is internationally recognized high quality food protein, be especially suitable in healthcare products and infant or baby food, it has good prospect in the development of food products market.
Rice residue is the residue after rice extracts syrup, and be the byproduct after rice sugar industryization is produced, in rice residue, protein content is up to 35%-40%, far away higher than protein content 7%-8% in rice.Rice protein in rice residue experienced by long High-temperature Liquefaction and amylorrhexis, produce crosslinked between molecule, protein and reducing sugar combine with N-cardohydrata-peptide linkage, the deep dark pigment generated makes rice residue in appearance in dark yellow, Starch Fraction is degraded, with the wrapped rice protein of limit dextrin.Therefore, in rice residue, rice protein solubleness greatly reduces and loses some associated functional property, limits its application in food, domestic mainly using after rice residue simple process as animal-feed, its added value is extremely low.
Through retrieval:
CN201110090550.3 discloses a kind of preparation method of high-purity rice protein, comprises and sizing mixing and defibrination, degreasing, saccharifying enzyme enzymolysis, separating, washing and the steps such as enzyme sterilizing of going out.
CN201110081635.5 discloses a kind of extracting method of protein of rice residue, and the method adopting alkali to propose acid heavy processes rice residue.
CN200910043181.5 discloses a kind of method of complex enzyme to prepare rice residue protein, wherein uses cellulase and amylase to process rice residue raw material.
CN200810163917.8 discloses a kind of method utilizing rice residue protein to produce rice protein polypeptide, alkali soak and Sumizyme MP, papoid united hydrolysis mode rice residue raw material is processed.
CN2012100390075 discloses a kind of method that rice residue prepares high-purity rice protein.
The people such as Chen Jiwang (enzyme preparation technique of rice peptide and the research [J] of characteristic thereof. Transactions of the Chinese Society of Agricultural Engineering, 2006,22 (6): 178-181.) research shows, utilize hydrolysis by novo rice protein, the rice peptide obtained has good solvability and lower viscosity, can widely use in food service industry.
The people such as Zheng Mai (development [J] of solubility corn protein powder. Jiangxi foodstuffs industry, 2006 (3): 34-35.) utilize compound protease to be hydrolyzed to corn protein powder, obtain the corn protein powder of solubility.
Many research shows, utilizes rice residue to be hydrolyzed to produce the protolysate that can be used for food to can yet be regarded as a kind of means of effective vegetable-protein.But due to rice residue complicated component, the extraction difficulty of albumen is comparatively large, the yield that the exploitation of the rice protein being exists product is lower, the problems such as purity is not high, and color and luster is darker.Alkalinity extraction rice gluten, protein yield is minimum, and solvability is also poor, is no longer applicable to the extraction of rice residue protein; How to prepare the technical barrier that the soluble hydrolysed rice albumen of high purity is the exploitation of a puzzlement rice protein.
Summary of the invention
In order to solve the problem, the invention provides the production method of the soluble hydrolysed rice albumen of a kind of high purity.Utilize rice residue as raw material, extract and obtain the high hydrolysed rice albumen of solvability high purity, realize suitability for industrialized production food method of rice protein hydrolysate, the market potential of further exploitation rice protein, expand its application within the scope of foodstuffs industry, and by the utilization to rice residue, promote rice deep processing variation and utilize.
The concrete technical scheme that the present invention adopts is:
1) rice residue pre-treatment: mixing solutions rice residue being mixed with massfraction 15-25%;
2) combinative enzyme hydrolysis: to step 1) add compound enzymic preparation, temperature 45-55 DEG C, pH7.0-8.0, sustained reaction 2-4h in the mixed solution that obtains, rear intensification should be terminated and to go out enzyme.
3) wash: filtration step 2) obtain mixed solution, and material slag is repeated washing, utilize filter press to remove water lotion;
4) enzymolysis hydrolysis: by step 3) washing after material slag add composite protease hydrolysis, temperature 50-65 DEG C, pH to 8.0-9.0, hydrolysis 2-4h; After hydrolysis terminates, heat up the enzyme that goes out.
5) decolour debitterize: to step 4) after retort in add 1-1.5% gac according to rice residue weight, stir decolouring 30-50min, be heated to 90-100 DEG C, decolouring after debitterize terminates, is cooled to normal temperature, filters to obtain hydrolyzed solution;
6) spraying dry: use spray-drying process to step 5) hydrolyzed solution that obtains carries out drying, obtains hydrolysed rice albumen finished product.
Described step 2) middle compound enzymic preparation is α-amylase, saccharifying enzyme, debranching enzyme combine.
Described compound enzymic preparation addition is α-amylase 30-40U/g, saccharifying enzyme 30-40U/g, debranching enzyme 30-40U/g, is preferably α-amylase 40U/g, saccharifying enzyme 30U/g, debranching enzyme 30U/g.
Step 4) in compound protease be Sumizyme MP and the combination of trypsinase two kinds of enzymes.
Described compound compound protease addition is 350-400U/g, and adding proportion is 2:1, and preferred protease addition is 350-400U/g, and adding proportion is 2:1.
Described step 6) in spray-dired processing condition be: feeding temperature: 40-60 DEG C, inlet temperature: 150-200 DEG C, carries out spraying dry under air outlet temperature: 60-85 DEG C condition.
The analysis of product purity and degree of hydrolysis:
In the present invention, the lipidated protein of product measures, referenced patent: publication number CN102524515A mono-kind prepares the method for high-purity rice protein by rice residue.
Degree of hydrolysis of the present invention measures, and with reference to people such as Yang Qian, rice protein enzyme hydrolysis condition and degree of hydrolysis are on the impact [J] of plastein. Hua Zhong Agriculture University's journal, 2007,26 (4): 565 ~ 569.
The present invention is used by the optimization of compound enzymic preparation, eliminate polymer starch and dextrin, the solubleness that improve rice protein and degree of hydrolysis in rice residue, reduce the molecular weight of method of rice protein hydrolysate, effectively improve purity and the quality of method of rice protein hydrolysate, product purity reaches 94% by mensuration; The degree of hydrolysis of product protein reaches 75%, and solubleness reaches 97%, and molecular-weight average is 880Da, and 85% concentrates on 790Da, and product emulsifying property and whipability are greatly improved.
Embodiment
The present invention's α-amylase used, saccharifying enzyme, debranching enzyme, Sumizyme MP, trypsinase, papoid is commercially available prod, without specificity requirement.
Embodiment 1
1) rice residue pre-treatment: by rice residue broken and cross 70 mesh sieves, dry.Take the mixing solutions that 100kg rice residue is mixed with massfraction 15% in damping fluid and join retort.2) combinative enzyme hydrolysis: to step 1) add compound enzymic preparation in the mixed solution that obtains, prozyme addition is α-amylase 40U/g, saccharifying enzyme 30U/g, debranching enzyme 30U/g.Temperature controls at 45 DEG C, adds concentration 0.3% alkali lye, and regulate pH7.0, sustained reaction 2h, be warming up to 80 DEG C after should terminating, go out enzyme 20min.3) wash: filtration step 2) obtain mixed solution, and material slag is repeated washing, utilize filter press to remove water lotion.4) enzymolysis hydrolysis: by step 3) washing after material slag be added in retort, add compound protease according to 350U/g rice residue weight, Sumizyme MP and trypsinase two kinds of enzyme adding proportions are 2:1.Add massfraction 0.3% alkali lye, regulate pH to 8.0, after stirring, temperature 55 DEG C, hydrolysis 4h.After hydrolysis terminates, be warming up to 80 DEG C, go out enzyme 20min.5) decolour debitterize: to step 4) after retort in add 1.0kg gac, stir decolouring 30min, be heated to 90 DEG C, decolouring after debitterize terminates, is cooled to normal temperature, by filter press removing material slag and gac, obtains hydrolyzed solution.6) spraying dry: use spray-drying process to step 5) hydrolyzed solution that obtains carries out drying, obtains hydrolysed rice albumen finished product.Spray-dired processing condition are: feeding temperature: 40 DEG C, inlet temperature: 150 DEG C, air outlet temperature: carry out spraying dry under 60 DEG C of conditions.
Adopt method of rice protein hydrolysate prepared by present method, product purity reaches 94% by mensuration; The degree of hydrolysis of product protein reaches 75%, and solubleness reaches 98%, and molecular-weight average is 830Da, and 85% concentrates on 790Da, and product emulsifying property and whipability are greatly improved.
Embodiment 2
1) rice residue pre-treatment: by rice residue broken and cross 70 mesh sieves, dry.Take the mixing solutions that 150kg rice residue is mixed with massfraction 25% in damping fluid and join retort.2) combinative enzyme hydrolysis: to step 1) add compound enzymic preparation in the mixed solution that obtains, preferred compound enzymic preparation is α-amylase, saccharifying enzyme, the combination of debranching enzyme prozyme.Prozyme addition is α-amylase 30U/g, saccharifying enzyme 40U/g, debranching enzyme 30U/g.Temperature controls at 55 DEG C, adds concentration 0.5% alkali lye, and regulate pH8.0, sustained reaction 30min, be warming up to 85 DEG C after should terminating, go out enzyme 20min.3) wash: filtration step 2) obtain mixed solution, and material slag is repeated washing, utilize filter press to remove water lotion.4) enzymolysis hydrolysis: by step 3) material slag after washing is added in retort, and add compound protease according to 400U/g rice residue weight, Sumizyme MP and the combination of trypsinase two kinds of enzymes, interpolation mass ratio is 2:1.Add massfraction 0.5% alkali lye, regulate pH to 9.0, after stirring, temperature 65 DEG C, hydrolysis 90min.After hydrolysis terminates, be warming up to 85 DEG C, go out enzyme 20min.5) decolour debitterize: to step 4) after retort in add 1.5kg gac, stir decolouring 50min, be heated to 100 DEG C, decolouring after debitterize terminates, is cooled to normal temperature, by filter press removing material slag and gac, obtains hydrolyzed solution.6) spraying dry: use spray-drying process to step 5) hydrolyzed solution that obtains carries out drying, obtains hydrolysed rice albumen finished product.Spray-dired processing condition are: feeding temperature: 60 DEG C, inlet temperature: 200 DEG C, air outlet temperature: carry out spraying dry under 85 DEG C of conditions.
Adopt method of rice protein hydrolysate prepared by present method, product purity reaches 93% by mensuration; The degree of hydrolysis of product protein reaches 75%, and solubleness reaches 96%, and molecular-weight average is 850Da, and 85% concentrates on 800Da, and product emulsifying property and whipability are greatly improved.
Embodiment 3
1) rice residue pre-treatment: by rice residue broken and cross 70 mesh sieves, dry.Take the mixing solutions that 125kg rice residue is mixed with massfraction 20% in damping fluid and join retort.2) combinative enzyme hydrolysis: to step 1) add compound enzymic preparation in the mixed solution that obtains, preferred compound enzymic preparation is α-amylase, saccharifying enzyme, the combination of debranching enzyme prozyme.Prozyme addition is α-amylase 40U/g, saccharifying enzyme 40U/g, debranching enzyme 30U/g.Temperature controls at 35-40 DEG C, adds concentration 0.4% alkali lye, and regulate pH8.0, sustained reaction 30min, be warming up to 85 DEG C after should terminating, go out enzyme 20min.3) wash: filtration step 2) obtain mixed solution, and material slag is repeated washing, utilize filter press to remove water lotion.4) enzymolysis hydrolysis: by step 3) material slag after washing is added in retort, and add compound protease according to 380U/g rice residue weight, Sumizyme MP and the combination of trypsinase two kinds of enzymes, interpolation mass ratio is 1:1.Add massfraction 0.4% alkali lye, regulate pH to 9.0, after stirring, temperature 50 C, hydrolysis 90min.After hydrolysis terminates, be warming up to 83 DEG C, go out enzyme 20min.5) decolour debitterize: to step 4) after retort in add 1.25kg gac, stir decolouring 40min, be heated to 95 DEG C, decolouring after debitterize terminates, is cooled to normal temperature, by filter press removing material slag and gac, obtains hydrolyzed solution.6) spraying dry: use spray-drying process to step 5) hydrolyzed solution that obtains carries out drying, obtains hydrolysed rice albumen finished product.Spray-dired processing condition are: feeding temperature: 50 DEG C, inlet temperature: 175 DEG C, air outlet temperature: carry out spraying dry under 72.5 DEG C of conditions.
Adopt method of rice protein hydrolysate prepared by present method, product purity reaches 93% by mensuration; The degree of hydrolysis of product protein reaches 70%, and solubleness reaches 94%, and molecular-weight average is 860Da, and 85% concentrates on 780Da, and product emulsifying property and whipability are greatly improved.
Reference examples 1
1) rice residue pre-treatment: by rice residue broken and cross 70 mesh sieves, dry.Take the mixing solutions that 150kg rice residue is mixed with massfraction 25% in damping fluid and join retort.2) combinative enzyme hydrolysis: to step 1) add compound enzymic preparation in the mixed solution that obtains, preferred compound enzymic preparation is α-amylase, saccharifying enzyme, the combination of debranching enzyme prozyme.Prozyme addition is α-amylase 30U/g, saccharifying enzyme 40U/g, debranching enzyme 30U/g.Temperature controls at 55 DEG C, adds concentration 0.5% alkali lye, and regulate pH8.0, sustained reaction 30min, be warming up to 85 DEG C after should terminating, go out enzyme 20min.3) wash: filtration step 2) obtain mixed solution, and material slag is repeated washing, utilize filter press to remove water lotion.4) enzymolysis hydrolysis: by step 3) material slag after washing is added in retort, and add compound protease according to 400U/g rice residue weight, Sumizyme MP and the combination of papoid two kinds of enzymes, interpolation mass ratio is 2:1.Add massfraction 0.5% alkali lye, regulate pH to 9.0, after stirring, temperature 55 DEG C, hydrolysis 90min.After hydrolysis terminates, be warming up to 85 DEG C, go out enzyme 20min.5) decolour debitterize: to step 4) after retort in add 1.5kg gac, stir decolouring 50min, be heated to 100 DEG C, decolouring after debitterize terminates, is cooled to normal temperature, by filter press removing material slag and gac, obtains hydrolyzed solution.6) spraying dry: use spray-drying process to step 5) hydrolyzed solution that obtains carries out drying, obtains hydrolysed rice albumen finished product.Spray-dired processing condition are: feeding temperature: 60 DEG C, inlet temperature: 200 DEG C, air outlet temperature: carry out spraying dry under 85 DEG C of conditions.
Adopt method of rice protein hydrolysate prepared by present method, product purity reaches 88% by mensuration; The degree of hydrolysis of product protein reaches 62%, and solubleness reaches 89%, and molecular-weight average is 1100Da, and 85% concentrates on 1020Da.
Reference examples 2
1) rice residue pre-treatment: by rice residue broken and cross 70 mesh sieves, dry.Take the mixing solutions that 100kg rice residue is mixed with massfraction 15-25% in damping fluid and join retort.2) enzymolysis hydrolysis: mixing solutions, add Sumizyme MP Sumizyme MP and trypsinase two kinds of enzyme combinations according to rice residue weight 1% ratio, adding proportion is 2:1.Add massfraction 0.3% alkali lye, regulate pH to 8.0, after stirring, temperature 45 C, hydrolysis 90min.After hydrolysis terminates, be warming up to 80 DEG C, go out enzyme 20min.After hydrolysis terminates, be warming up to 80 DEG C, go out enzyme 20min.3) decolour debitterize: to step 2) after retort in add 1.0kg gac, stir decolouring 30min, be heated to 90 DEG C, decolouring after debitterize terminates, is cooled to normal temperature, by filter press removing material slag and gac, obtains hydrolyzed solution.4) spraying dry: use spray-drying process to step 3) hydrolyzed solution that obtains carries out drying, obtains hydrolysed rice albumen finished product.Spray-dired processing condition are: feeding temperature: 40 DEG C, inlet temperature: 150 DEG C, air outlet temperature: carry out spraying dry under 60 DEG C of conditions.
Adopt method of rice protein hydrolysate prepared by present method, product purity is by being determined as 72%; The degree of hydrolysis of product protein reaches 58%, and solubleness reaches 78%, and molecular-weight average is 1500Da, and 85% concentrates on 1380Da.
Claims (8)
1. a production method for the soluble hydrolysed rice albumen of high purity, is characterized in that comprising following steps:
1) rice residue pre-treatment: mixing solutions rice residue being mixed with massfraction 15-25%;
2) combinative enzyme hydrolysis: to step 1) add compound enzymic preparation, temperature 45-55 DEG C, pH7.0-8.0, sustained reaction 2-4h in the mixed solution that obtains, rear intensification should be terminated and to go out enzyme;
3) wash: filtration step 2) obtain mixed solution, and material slag is repeated washing, utilize filter press to remove water lotion;
4) enzymolysis hydrolysis: to step 3) washing after material slag according to rice residue weight add composite protease hydrolysis, temperature 50-65 DEG C, pH to 8.0-9.0, hydrolysis 2-4h; After hydrolysis terminates, heat up the enzyme that goes out;
5) decolour debitterize: to step 4) after retort in add 1-1.5% gac according to rice residue weight, stir decolouring 30-50min, be heated to 90-100 DEG C, decolouring after debitterize terminates, is cooled to normal temperature, filters to obtain hydrolyzed solution;
6) spraying dry: use spray-drying process to step 5) hydrolyzed solution that obtains carries out drying, obtains hydrolysed rice albumen finished product.
2. method according to claim 1, is characterized in that step 2) in compound enzymic preparation be the combination of α-amylase, saccharifying enzyme, debranching enzyme.
3. method according to claim 1, is characterized in that described prozyme addition is α-amylase 30-40U/g, saccharifying enzyme 30-40U/g, debranching enzyme 30-40U/g.
4. method according to claim 3, is characterized in that described prozyme addition is preferably α-amylase 40U/g, saccharifying enzyme 30U/g, debranching enzyme 30U/g.
5. method according to claim 1, is characterized in that step 4) in compound protease be Sumizyme MP and the combination of trypsinase two kinds of enzymes.
6. method according to claim 5, it is characterized in that described compound protease addition is 350-400U/g rice residue, Sumizyme MP and trypsinase adding proportion are 2:1.
7. method according to claim 6, it is characterized in that described compound protease addition is 350U/g rice residue, adding proportion is 2:1.
8. method according to claim 1, is characterized in that step 6) in spray-dired processing condition be: feeding temperature: 40-60 DEG C, inlet temperature: 150-200 DEG C, carries out spraying dry under air outlet temperature: 60-85 DEG C condition.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106561971A (en) * | 2016-11-07 | 2017-04-19 | 南昌大学 | Rice protein peptide and preparation method thereof |
| CN109468356A (en) * | 2018-11-22 | 2019-03-15 | 湖南汇升生物科技有限公司 | A kind of preparation method of rice protein |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101530153A (en) * | 2009-04-22 | 2009-09-16 | 湖南农业大学 | Method by adopting complex enzyme to prepare rice residue protein |
| CN101695334A (en) * | 2009-09-09 | 2010-04-21 | 万福生科(湖南)农业开发股份有限公司 | Method for extracting rice protein by using rice |
| CN103229887A (en) * | 2013-04-25 | 2013-08-07 | 合肥锦泰糖业有限公司 | Method for preparing rice protein by utilizing bio-enzyme |
| CN103918869A (en) * | 2014-04-24 | 2014-07-16 | 盘锦和田食品有限公司 | High-purity rice protein peptide and preparation method thereof |
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| CN101530153A (en) * | 2009-04-22 | 2009-09-16 | 湖南农业大学 | Method by adopting complex enzyme to prepare rice residue protein |
| CN101695334A (en) * | 2009-09-09 | 2010-04-21 | 万福生科(湖南)农业开发股份有限公司 | Method for extracting rice protein by using rice |
| CN103229887A (en) * | 2013-04-25 | 2013-08-07 | 合肥锦泰糖业有限公司 | Method for preparing rice protein by utilizing bio-enzyme |
| CN103918869A (en) * | 2014-04-24 | 2014-07-16 | 盘锦和田食品有限公司 | High-purity rice protein peptide and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106561971A (en) * | 2016-11-07 | 2017-04-19 | 南昌大学 | Rice protein peptide and preparation method thereof |
| CN109468356A (en) * | 2018-11-22 | 2019-03-15 | 湖南汇升生物科技有限公司 | A kind of preparation method of rice protein |
| CN109468356B (en) * | 2018-11-22 | 2021-03-26 | 湖南汇升生物科技有限公司 | Preparation method of rice protein |
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