CN105534877A - Fermentation traditional Chinese medicine combination for liquid shampoo and preparation method and application of combination - Google Patents
Fermentation traditional Chinese medicine combination for liquid shampoo and preparation method and application of combination Download PDFInfo
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Abstract
The invention discloses a fermentation traditional Chinese medicine combination for liquid shampoo and a preparation method of the combination. The fermentation traditional Chinese medicine combination is prepared after traditional Chinese medicines of lightyellow sophora root, safflower flower, common cnidium fruit and pricklyash peel are fermented by bacillus subtilis and yeaster, combined with amur corktree bark, rhizome of Chinese goldthread and baical skullcap root extracts and added into a chitosan-acetic acid solution. The fermentation traditional Chinese medicine combination can be used for preparing anti-scurf shampoo products. Experiments show that the shampoo products comprising the fermentation traditional Chinese medicine combination have remarkable inhibiting effects on malassezia fufur and remarkable efficacy for curing seborrheic dermatitis.
Description
Technical field
The present invention relates to the application of herb fermenting compositions in household chemicals field that bacillus subtilis, yeast fermentation Chinese medicine and Chinese medicine extract are prepared jointly, belong to technical field of biological fermentation.
Background technology
The dandruff is scalp common disease, is medically called " capitis pityriasis ", and clinical manifestation is too much ash fines white dried or slightly greasy pityrosporum sample chip on scalp or hair, and can accompany pruritus, crowd's sickness rate is high.Modern medicine study shows, formed abnormal dandruff be because a kind of reside in human skin addicted to fat yeast-like fungi.Under normal circumstances, the dandruff of natural metabolism is also not easy to observe, but, if scalp metabolism is too fast, fat secretion is unbalance, or when cell mature process is incomplete, cutin will be made to pile up, now with the chlosma spore amount reproduction that scalp dead cell is supported one's family, cause cutin to come off, the dandruff as snowflake also just creates.Current most dandruff-controlling shampoo is mostly containing chemical anti-dandruff agent, antibacterial, and as compositions such as ketoconazole, zinc pyrithione, climbazoles, chemical composition is comparatively large to scalp injury, and easily produces drug resistance.The anti-chlosma shampoo of exploitation high-efficiency low-toxicity just seems particularly important, but synthesizes a kind of new antifungal platform thing, and cycle long labor intensive material resources are huge.The treatment of China's Chinese medicine to fungal infection is with a long history, have accumulated rich experiences, from Chinese herbal medicine and extract thereof, therefore filters out the medicine with anti-chlosma effect, make shampoo, can lower the untoward reaction of chemical composition, prevents drug resistance from occurring.
Summary of the invention
Microorganism has very abundant enzyme system, has powerful decomposition, the ability of transformation substance.Use fermentable Chinese medicine, biology can be carried out to chemical composition of Chinese materia medica and transform, produce new compound or cause the change of some component contents in Chinese medicine.In addition, in the process of fermentable Chinese medicine, some compositions in Chinese medicine can some metabolic pathway of inducing microbial change, thus produce new compound.Further, occurring in nature, also also exists biological antagonist relation between microorganism, and the specific metabolite that certain Institute of Micro-biology produces can suppress the growth of other microbial growths even to kill them.
The invention provides the application of herb fermenting compositions in household chemicals field that a kind of bacillus subtilis, yeast fermentation Chinese medicine and Chinese medicine extract are prepared jointly, particularly relate to Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli after bacillus subtilis, yeast fermentation, mix with Cortex Phellodendri, Rhizoma Coptidis, Radix Scutellariae extracting solution, through extracting, obtained fermented tcm compositions, this fermented tcm compositions is for the preparation of the application in dandruff-controlling shampoo.
An object of the present invention is to provide a kind of fermented tcm compositions for shampoo, said composition forms compound recipe by sophora flavescens ait, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli, Cortex Phellodendri, Rhizoma Coptidis, Radix Scutellariae, obtains by the following method:
A. get Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli pulverizing, cross 40 mesh sieves, add the water of medical material weight 10 times, soak 1.0 hours, adjust pH is 7.0,115 DEG C of sterilizings 50 minutes;
B. when temperature is down to 33 DEG C, the bacillus cereus bacterium liquid that access preculture is good, inoculum concentration is volume ratio 3%, cultivates 20 ~ 24 hours, then accesses the good at least one strain above yeast bacterium liquid of preculture for 32 DEG C, every strain bacterium inoculum concentration is volume ratio 2%, cultivation temperature 30 DEG C, ferments 24 ~ 36 hours, is heated to 100 DEG C, and keep 10 minutes, centrifugally obtain supernatant;
Precipitation medicinal residues after centrifugal are added 85% ethanol of 10 times of weight, heating and refluxing extraction 1 hour, filter, decompression filtrate recycling ethanol, alcohol extract and the centrifugal supernatant obtained merge, and obtain four taste herb fermenting extracting solution; Or: the not alcohol extraction of precipitation medicinal residues, directly discards;
C. Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae soak 1 hour, decoct twice, each 1 hour, add the water of 10 times of medical material amounts for the first time, the water of second time 8 times of medical material amounts, and gradation filters, merging filtrate;
D. the four taste herb fermenting extracting solution filtrate of step C and step B obtained or supernatant merge, being evaporated to medical material weight g/ volume ml ratio is 20%, by medicine liquid heating to 80 DEG C, under stirring, slowly add the chitosan-acetic acid solution of 1% concentration, addition is 12% of medicine liquid volume; Sedimentation is after 8 ~ 12 hours, centrifugal, supernatant continue to be evaporated to medical material content be weight g/ volume ml than 50%, extracting solution precipitate with ethanol or not precipitate with ethanol, obtain fermented tcm compositions;
Described Traditional Chinese Medicine dosage is: Radix Sophorae Flavescentis 10-20 part, Flos Carthami 5-16 part, Fructus Cnidii 6-15 part, Fructus Zanthoxyli 5-16 part, Cortex Phellodendri 6-16 part, Rhizoma Coptidis 15-25 part, Radix Scutellariae 6-15g part.
Cortex Phellodendri is the dry bark of rutaceae wampee PhellodendronchinenseSchneid..Bitter in the mouth, cold in nature, have heat clearing and damp drying, pathogenic fire purging removes steams, effect of detoxification sore treatment.Modern study shows, Cortex Phellodendri contains antibiotic effective ingredient berberine, and in vitro tests shows, all has inhibitory or killing effect to a certain degree to antibacterial, fungus.Rhizoma Coptidis is the dry rhizome of ranunculaceae plant Rhizoma Coptidis CoptischinensisFranch., Coptis deltoidea C.Y.Cheng et Hsiao CoptisdeltoideaC.Y.ChengetHsiao or Coptis Teeta Wall CoptisteetaWall., bitter in the mouth, cold.GUIXIN, spleen, stomach, liver, gallbladder, large intestine channel.There is heat clearing and damp drying, the function of eliminating fire and detoxication.Modern pharmacological research shows, Rhizoma Coptidis has the effect suppressing sex pheromone.Radix Scutellariae (Classification system: ScutellariabaicalensisGeorgi), another name Radix Scutellariae, tsuchikane tea root, be Labiatae Scutellaria herbaceos perennial, the root of Radix Scutellariae is used as medicine, bitter in the mouth, cold in nature, there are heat clearing and damp drying, eliminating fire and detoxication, hemostasis, the effect such as antiabortive.Radix Scutellariae extracting solution has the effect of antibacterium, fungus, virus and antiinflammatory antiallergic action.Radix Sophorae Flavescentis is the dry root of leguminous plant Radix Sophorae Flavescentis SophoraflavescensAit..Nature and flavor are bitter, and cold, GUIXIN, liver, stomach, large intestine, urinary bladder channel, pharmacological action shows, has diuresis, antiviral effect.Flos Carthami is the dried floral of feverfew Flos Carthami CarthamustinctoriusL..Pluck when summer, flower was red by xanthochromia and get tubular flower, nature and flavor are pungent, temperature.Enter the heart, Liver Channel.There is promoting blood circulation to restore menstrual flow, the effect of eliminating stasis to stop pain.Fructus Cnidii Cnidiummonnieri(L.) Cuss., another name Daucus carota L. is the dry mature fruit of samphire cnidium monnieri.Gather when summer, autumn two season fruit maturation, removing impurity, dries.Warm in nature, bitter in the mouth.Return kidney channel.There is warming the kidney to invigorate YANG, dampness, dispel the wind, the effect of parasite killing.Fructus Zanthoxyli is the dry mature skin of rutaceae Capsicum annuum L. (fragrant green pepper, Zanthoxylum schinifolium, Pericarpium Zanthoxyli, Canis familiaris L. green pepper) ZanthoxylumschinifoliumSieb.etZucc. or Pericarpium Zanthoxyli (another name for Sichuan Province green pepper, Fructus Zanthoxyli, Capsicum tetragonum, pericarpium zanthoxyli bungeani, Radix Campylotropis Hirtella (Herba Myrsines Africanae)) ZanthoxylumbungeanumMaxim..Nature and flavor are pungent, numb, temperature.Return spleen, stomach, kidney channel, have inhibitory action to various pathogens and some dermatophytes.
Preferably, described Traditional Chinese Medicine dosage is: Radix Sophorae Flavescentis 13 parts, 10 parts, Flos Carthami, Fructus Cnidii 12 parts, Fructus Zanthoxyli 16 parts, Cortex Phellodendri 10 parts, Rhizoma Coptidis 25 parts, Radix Scutellariae 14 parts.
According to the present invention, described bacillus cereus comprises Bacillus subtilis subspecies Bacillussubtilissubsp.Subtilis(AS1.210), Bacillus subtilis natto, yeast comprises reddish brown shadow yeast Sporobolomycessalmonicolor(AS2.2114), Saccharomyces kluyveri Saccharomyceskluyveri(AS2.4070), Saccharomyces Cerevisiae in S accharomycescerevisiae(AS2.3852).
Described chitosan is the chitosan solution of 1% concentration prepared with the acetum of 1% concentration, and imbibition uses after spending the night.
The step of described precipitate with ethanol adds ethanol, and make ethanol final concentration be 75%, sedimentation is spent the night, and filter, decompression recycling ethanol, it is 50%(weight/volume that residual liquid is settled to medical material content).
Another object of the present invention is to provide fermented tcm compositions of the present invention and applies in daily use chemicals product, and it as adding ingredient, can be applied to the production of shampoo.
Described shampoo is made up of shampoo substrate 95 parts, fermented tcm compositions 5 parts.
Described shampoo substrate is: 58.55 parts, water, laureth sulfuric ester ammonium 11 parts, lauryl alcohol sulfuric ester ammonium 7 parts, methyl hydroxybenzoate 0.1 part, propyl hydroxybenzoate 0.05 part, EDETATE SODIUM 0.1 part, coconut oleoyl amine MEA1.5 part, two (hydrogenated-tallow group) phthalic acid amide 0.5 part, glycol distearate 2 parts, guar hydroxypropyltrimonium chloride 0.3 part, VARISOFT TA100, cetrimonium chloride 0.4 part, piroctone olamine salt 0.7 part, citric acid 0.02 part, cocamido propyl betaine 4 parts, Polyethylene Glycol-14M0.06 part, glucose, glycerol polymethacrylates, dodecane hexadecyltrimethylammonium chloride 2 parts, pantothenylol 0.2 part, polyquaternary ammonium salt-70.5 parts, polydimethylsiloxane, cocamido propyl betaine, C12-15 Pareth-3, guar hydroxypropyltrimonium ammonium chloride 2 parts, dimethiconol, DBSA TEA salt 1.5, hyaluronate sodium 0.01 part, yeast extract 1 part, algae extract 1 part, 0.25 part, essence, ammonium chloride 0.2 part, magnesium nitrate, methylchloroisothiazandnone, Methylisothiazolinone 0.06 part.Its preparation method is as follows:
A. successively the water of room temperature, laureth sulfuric ester ammonium, lauryl alcohol sulfuric ester ammonium are added manufacture tank, be stirred to raw material and all dissolve;
B. Polyethylene Glycol-14M, VARISOFT TA100, cetrimonium chloride, two (hydrogenated-tallow group) phthalic acid amide, methyl hydroxybenzoate, propyl hydroxybenzoate, EDETATE SODIUM is added successively, be stirred and heated to 80 DEG C, stop heating, be incubated 10 minutes, start cooling;
C. greenhouse cooling is to 70 DEG C, add coconut oleoyl amine MEA, glycol distearate successively, add after the water dispersed with stirring of guar hydroxypropyltrimonium chloride and 10 times, add piroctone olamine salt, dispersed with stirring is even, add citric acid, cocamido propyl betaine, glucose, glycerol polymethacrylates, dodecane hexadecyltrimethylammonium chloride, fermented tcm compositions, pantothenylol, polyquaternary ammonium salt-7, cool to 45 DEG C;
D. add other raw materials successively, stirring and dissolving is even, to obtain final product.
Beneficial effect: for detecting fermented tcm compositions of the present invention to the inhibitory action of Malassezia furfur and the anti-dandruff effect of shampoo containing fermented tcm compositions of the present invention, the laboratory observation minimal inhibitory concentration of fermented tcm compositions to Malassezia furfur, infect the therapeutical effect of the animal model of Malassezia furfur and have dandruff volunteer to use the shampoo effect containing fermented tcm compositions of the present invention, experimental result shows: fermented tcm compositions≤5% concentration of the present invention can suppress the growth of Malassezia furfur preferably, and, Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, medicinal residues after Fructus Zanthoxyli four taste herb fermenting are again through alcohol extraction, the fungistatic effect of fermented tcm compositions can be improved, in the 4th day, the 7th day statistical result showed of guinea pig skin chlosma infection place medication, negative control group, blank group is obviously less than containing fermented tcm compositions laboratory sample Malassezia furfur clump count of the present invention, have significant difference (* P<0.01), the fungus negative conversion rate of fermentation composition of the present invention is between 20 ~ 70%, having dandruff volunteer to use containing after fermented tcm compositions shampoo of the present invention, effective percentage reaches 91%.
Detailed description of the invention
Hereinafter with reference to embodiment, the present invention is conducted further description, but should not regard limitation of the present invention as.
The preparation of embodiment 1 fermented tcm compositions A
Ingredients is taken: Radix Sophorae Flavescentis 18g according to following weight, Flos Carthami 13g, Fructus Cnidii 13g, Fructus Zanthoxyli 12g, Rhizoma Coptidis 20g, Cortex Phellodendri 14g, Radix Scutellariae 10g, get Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli is pulverized, cross 40 mesh sieves, add the water of medical material weight 10 times, soak 1.0 hours, adjust pH is 7.0, 115 DEG C of sterilizings 50 minutes, when temperature is down to 33 DEG C, Bacillus subtilis subspecies bacterium liquid (AS1.210) that access preculture is good, inoculum concentration is 3%(volume/volume), cultivate 22 hours for 32 DEG C, access the reddish brown shadow yeast (AS2.2114) that preculture is good again, saccharomyces cerevisiae (AS2.3852) bacterium bacterium liquid, inoculum concentration is 2%(volume/volume) cultivation temperature 30 DEG C, ferment 30 hours, be heated to 100 DEG C, and keep 10 minutes, centrifuging and taking supernatant, for subsequent use.Precipitation is added 85% ethanol of 10 times of medicinal residues weight, heating and refluxing extraction 1 hour, filter, decompression filtrate recycling ethanol, alcohol extract merges with fermentation centrifuged supernatant, obtains four Chinese medicine fermented extracted liquid.Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae soak 1 hour, decoct twice, each 1 hour, add the water of 10 times of medical material amounts for the first time, and the water of second time 8 times of medical material amounts, gradation filters, merging filtrate.Filtrate and four Chinese medicine fermented extracted liquid are merged, and be evaporated to medical material concentration 20%(medical material weight g/ volume ml), by medicine liquid heating to 80 DEG C, under stirring, slowly add 1%(weight g/ volume ml) chitosan-acetic acid solution of concentration, addition is 12% of medicine liquid volume.Sedimentation is after 10 hours, centrifugal, it is 50%(medical material weight g/ volume ml that supernatant continues to be evaporated to medical material content), by fermented tcm compositions precipitate with ethanol, make ethanol final concentration be 75%, sedimentation is spent the night, filter, reclaim ethanol, it is 50%(medical material weight g/ volume ml that residual liquid is settled to medical material content), obtain fermented tcm compositions A.
The preparation of embodiment 2 fermented tcm compositions B
Ingredients is taken: Radix Sophorae Flavescentis 18g according to following weight, Flos Carthami 13g, Fructus Cnidii 13g, Fructus Zanthoxyli 12g, Rhizoma Coptidis 20g, Cortex Phellodendri 14g, Radix Scutellariae 10g, get Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli is pulverized, cross 40 mesh sieves, add the water of medical material weight 10 times, soak 1.0 hours, adjust pH is 7.0, 115 DEG C of sterilizings 50 minutes, when temperature is down to 33 DEG C, Bacillus subtilis subspecies bacterium liquid (AS1.210) that access preculture is good, inoculum concentration is 3%(volume/volume), cultivate 22 hours for 32 DEG C, access the reddish brown shadow yeast (AS2.2114) that preculture is good again, saccharomyces cerevisiae (AS2.3852) bacterium bacterium liquid, inoculum concentration is 2%(volume/volume) cultivation temperature 30 DEG C, ferment 30 hours, be heated to 100 DEG C, and keep 10 minutes, centrifuging and taking supernatant, for subsequent use, obtain four Chinese medicine fermented extracted liquid.Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae soak 1 hour, decoct twice, each 1 hour, add the water of 10 times of medical material amounts for the first time, and the water of second time 8 times of medical material amounts, gradation filters, merging filtrate.Filtrate and four Chinese medicine fermented extracted liquid are merged, and be evaporated to medical material concentration 20%(medical material weight g/ volume ml), by medicine liquid heating to 80 DEG C, under stirring, slowly add 1%(weight g/ volume ml) chitosan-acetic acid solution of concentration, addition is 12% of medicine liquid volume.Sedimentation is after 10 hours, centrifugal, it is 50%(medical material weight g/ volume ml that supernatant continues to be evaporated to medical material content), by fermented tcm compositions precipitate with ethanol, make ethanol final concentration be 75%, sedimentation is spent the night, filter, reclaim ethanol, it is 50%(medical material weight/volume that residual liquid is settled to medical material content), obtain fermented tcm compositions B.
The preparation of embodiment 3 fermented tcm compositions C
Ingredients is taken: Radix Sophorae Flavescentis 13g according to following weight, Flos Carthami 10g, Fructus Cnidii 12g, Fructus Zanthoxyli 16g, Rhizoma Coptidis 25g, Cortex Phellodendri 10g, Radix Scutellariae 14g, get Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli is pulverized, cross 40 mesh sieves, add the water of medical material weight 10 times, soak 1.0 hours, adjust pH is 7.0, 115 DEG C of sterilizings 50 minutes, when temperature is down to 33 DEG C, the bacillus subtilis (being separated in natto) that access preculture is good, inoculum concentration is 3%(volume/volume), cultivate 24 hours for 32 DEG C, access the good Saccharomyces kluyveri of preculture (AS2.4070) again, saccharomyces cerevisiae (AS2.3852) bacterium bacterium liquid, inoculum concentration is 2%(volume/volume) cultivation temperature 30 DEG C, ferment 36 hours, be heated to 100 DEG C, and keep 10 minutes, centrifuging and taking supernatant, for subsequent use.Precipitation is added 85% ethanol of 10 times of medicinal residues weight, heating and refluxing extraction 1 hour, filter, decompression filtrate recycling ethanol, alcohol extract merges with fermentation centrifuged supernatant, obtains four Chinese medicine fermented extracted liquid.Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae soak 1 hour, decoct twice, each 1 hour, add the water of 10 times of medical material amounts for the first time, and the water of second time 8 times of medical material amounts, gradation filters, merging filtrate.Filtrate and four Chinese medicine fermented extracted liquid are merged, and be evaporated to medical material concentration 20%(medical material weight g/ volume ml), by medicine liquid heating to 80 DEG C, under stirring, slowly add 1%(weight g/ volume ml) chitosan-acetic acid solution of concentration, addition is 12% of medicine liquid volume.Sedimentation is after 12 hours, centrifugal, it is 50%(medical material weight g/ volume ml that supernatant continues to be evaporated to medical material content), by fermented tcm compositions precipitate with ethanol, make ethanol final concentration be 75%, sedimentation is spent the night, filter, reclaim ethanol, it is 50%(medical material weight g/ volume ml that residual liquid is settled to medical material content), obtain fermented tcm compositions C.
The preparation of embodiment 4 fermented tcm compositions D
Ingredients is taken: Radix Sophorae Flavescentis 10g according to following weight, Flos Carthami 15g, Fructus Cnidii 7g, Fructus Zanthoxyli 16g, Rhizoma Coptidis 25g, Cortex Phellodendri 10g, Radix Scutellariae 14g, get Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli is pulverized, cross 40 mesh sieves, add the water of medical material weight 10 times, soak 1.0 hours, adjust pH is 7.0, 115 DEG C of sterilizings 50 minutes, when temperature is down to 33 DEG C, the bacillus subtilis (being separated in natto) that access preculture is good, inoculum concentration is 3%(volume/volume), cultivate 24 hours for 32 DEG C, access the good Saccharomyces kluyveri of preculture (AS2.4070) again, saccharomyces cerevisiae (AS2.3852) bacterium bacterium liquid, inoculum concentration is 2%(volume/volume) cultivation temperature 30 DEG C, ferment 36 hours, be heated to 100 DEG C, and keep 10 minutes, centrifuging and taking supernatant, for subsequent use.Precipitation is added 85% ethanol of 10 times of medicinal residues weight, heating and refluxing extraction 1 hour, filter, decompression filtrate recycling ethanol, alcohol extract merges with fermentation centrifuged supernatant, obtains four Chinese medicine fermented extracted liquid.Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae soak 1 hour, decoct twice, each 1 hour, add the water of 10 times of medical material amounts for the first time, and the water of second time 8 times of medical material amounts, gradation filters, merging filtrate.Filtrate and four Chinese medicine fermented extracted liquid are merged, and be evaporated to medical material concentration 20%(medical material weight g/ volume ml), by medicine liquid heating to 80 DEG C, under stirring, slowly add 1%(weight g/ volume ml) chitosan-acetic acid solution of concentration, addition is 12% of medicine liquid volume.Sedimentation is after 12 hours, centrifugal, and it is 50%(medical material weight g/ volume ml that supernatant continues to be evaporated to medical material content), obtain fermented tcm compositions D.
Embodiment 5 is for the anti-dandruff preparation containing fermented tcm compositions shampoo of the present invention
Formula (wherein each Ingredients Weight unit be kilogram)
| Sequence number | Title | Weight (kilogram) |
| 1 | Water | 58.55 |
| 2 | Laureth sulfuric ester ammonium | 11 |
| 3 | Lauryl alcohol sulfuric ester ammonium | 7 |
| 4 | Methyl hydroxybenzoate | 0.1 |
| 5 | Propyl hydroxybenzoate | 0.05 |
| 6 | EDTA disodium | 0.1 |
| 7 | Coconut oleoyl amine MEA | 1.5 |
| 8 | Two (hydrogenated-tallow group) phthalic acid amide | 0.5 |
| 9 | Glycol distearate | 2 |
| 10 | Guar hydroxypropyltrimonium chloride | 0.3 |
| 11 | VARISOFT TA100, cetrimonium chloride | 0.4 |
| 12 | Piroctone olamine salt | 0.7 |
| 13 | Citric acid | 0.02 |
| 14 | Cocamido propyl betaine | 4 |
| 15 | Polyethylene Glycol-14M | 0.06 |
| 16 | Glucose, glycerol polymethacrylates, dodecane hexadecyltrimethylammonium chloride | 2 |
| 17 | Fermented tcm compositions | 5 |
| 18 | Pantothenylol | 0.2 |
| 19 | Polyquaternary ammonium salt-7 | 0.5 |
| 20 | Polydimethylsiloxane, cocamido propyl betaine, C12-15 Pareth-3, guar hydroxypropyltrimonium ammonium chloride | 2 |
| 21 | Dimethiconol, DBSA TEA salt | 1.5 |
| 22 | Hyaluronate sodium | 0.01 |
| 23 | Yeast extract | 1 |
| 24 | Algae extract | 1 |
| 25 | Essence | 0.25 |
| 26 | Ammonium chloride | 0.2 |
| 27 | Magnesium nitrate, methylchloroisothiazandnone, Methylisothiazolinone | 0.06 |
Preparation method: successively 1,2, of room temperature No. 3 raw material is added manufacture tank, start stirring, be stirred to raw material and all dissolve, add 15,11,8,4,5, No. 6 raw materials successively, open heating.Be stirred and heated to 80 DEG C, stop heating.Be incubated 10 minutes, start cooling, cool to 70 DEG C, add 7, No. 9 raw materials successively, add after the water dispersed with stirring of No. 10 raw materials and 10 times, add No. 12 raw materials, dispersed with stirring is even, adds 13,14,16,17,18, No. 19 raw materials, cool to 45 DEG C, add other raw materials successively, stirring and dissolving is even, namely obtains for the anti-dandruff shampoo containing fermented tcm compositions.
The fermented tcm compositions added in preparation process refers to fermented tcm compositions A or B or C or D, namely obtains the shampoo containing fermented tcm compositions A or B or C or D.
Experimental example 1 fermented tcm compositions is to the Inhibition test of Malassezia furfur
Laboratory sample is fermented tcm compositions A, B, C, D tetra-samples prepared by embodiment 1-4.
Experimental technique:
1, Malassezia furfur culture medium: glucose 40g, peptone 10g, agar 15g, distilled water 1000ml, 40ml Oleum Sesami, 10ml Tween 80.
2, the preparation of bacteria suspension
The lawn cultivating the Malassezia furfur inclined-plane of the activation of transferred species of 4 days with picking 32 DEG C after the calcination of inoculating loop flame is appropriate, and insert the physiological saline solution of sterile glass beads, jolting becomes suspension, and with blood counting chamber counting, controlling bacteria suspension concentration is lxl0
6cFU/ml.
3, the preparation of test sample: four laboratory samples are diluted to 5 Concentraton gradient with sterile distilled water respectively, be respectively 1.25(volume/volume), 2.5%(volume/volume), 5.0%(volume/volume), 7.5%(volume/volume), 10%(volume/volume), with distilled water in contrast.
4, minimum inhibitory concentration (MIC) experiment
(1) pastille culture medium is prepared: aseptically, get the diluent 0.3ml of four sample variable concentrations respectively, join in sterile test tube, when sterilising medium after the optimization prepared is cooled to 40 DEG C ~ 50 DEG C, sterile working adds often props up test tube 2.7ml, mixing bevel.Often pipe final volume is 3ml.
(2) bacteria suspension is inoculated: be respectively inoculated in respectively in the medium slant of pastille base by the Malassezia furfur prepared, bacteria suspension addition 200ul.
(3) cultivate: oneself inoculation Malassezia furfur pastille culture medium is placed in 32 DEG C of calorstats and cultivates 4 days.
(4) interpretation of MIC value: naked eyes carry out interpretation under available light after cultivation terminates.Under negative control pipe culture medium clearly prerequisite, positive control pipe colony growth is good simultaneously, carries out result interpretation.By pastille culture tube often pipe growing state and control tube compare, be MIC terminal by the lowest concentration of drug pastille culture tube grown without culture, so, the drug level of this pastille culture tube is the MIC value of this test sample to this bacterial strain.Specific experiment the results are shown in Table 1.
The Inhibition test that table 1 fermented tcm compositions grows Malassezia furfur
Illustrate :-do not grow for Malassezia furfur; + be Malassezia furfur growth.
As can be seen from Table 1, the minimal inhibitory concentration of fermented tcm compositions A is 2.5%, the minimal inhibitory concentration of fermented tcm compositions B is 5.0%, in preparation technology, fermented tcm compositions A is compared to fermented tcm compositions B, and the medicinal residues after Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli fermentation, again through alcohol extraction, this shows, medicinal residues, through alcohol extraction, can improve the bacteriostatic activity of fermentation composition.The minimal inhibitory concentration of fermentation composition C is 2.5%, the minimal inhibitory concentration of fermentation composition D is <1.25%, in preparation technology, fermented tcm compositions D is compared to fermented tcm compositions C, last without alcohol precipitation process, the Mlc of fermentation composition D, lower than fermentation composition C, illustrates that precipitate with ethanol can lose a part of effective ingredient.But the clarity of fermentation composition D is lower than fermentation composition C.
The zoopery that the anti-skin chlosma of experimental example 2 fermented tcm compositions infects
Laboratory sample is fermented tcm compositions A, B, C, D tetra-samples prepared by embodiments of the invention 1,2,3,4.
Experimental technique
1, Malassezia furfur culture medium: glucose 40g, peptone 10g, agar 15g, distilled water 1000ml, 40ml Oleum Sesami, 10ml Tween 80.
2, the preparation of bacteria suspension: the continuous passage on solid medium of Malassezia furfur type strain is cultivated 2 times, to ensure its vigor.2nd 32 DEG C of cultivations are after 5 days, and choose its bacterium colony in aseptic 0.9% normal saline that bead is housed, jolting repeatedly, makes bacteria suspension, and blood cell counts plate counts, and adjusting its final concentration is 1 × IO
8cFU/m1.
3, the preparation of medicine: each laboratory sample is made containing 5%(volume/volume) the vaseline cream of fermented tcm compositions of concentration, negative control thing is simple vaseline frost, the place's not medication of blank group skin lesion.
4, the structure of guinea pig skin chlosma infected animal model:
(1) hair shaved by guinea pig back razor, with depilatory wax depilation, formed 4.5 × 7.5 square centimeters without hair-fields.
(2) carry out the direct microscopy of fungus and cultivation to guinea pig back without hair-fields, result is feminine gender.
(3) rub equably gently at Cavia porcellus back with sand paper after 24h, and bacteria suspension 200ul is evenly applied to back without hair-fields.
(4) bacterium 1 time is coated with every day, continuous 7 days.
5, the outer skin infected for guinea pig back chlosma of medicine.
(1) Cavia porcellus Self-control method is adopted
(2) experiment is divided into two groups, and often organize each 10 Cavia porcelluss, often group is divided into again 6 groups according to medication difference, 6 group's medication instructions:
1st group: treatment group, the external of skin lesion place is containing the cream of 5% fermented tcm compositions A;
2nd group: treatment group, the external of skin lesion place is containing the cream of 5% fermented tcm compositions B;
3rd group: treatment group, the external of skin lesion place is containing the cream of 5% fermented tcm compositions C;
4th group: treatment group, the external of skin lesion place is containing the cream of 5% fermented tcm compositions D;
5th group: negative control group, the simple cream base of skin lesion place external;
6th group: blank group, skin lesion place not external used medicine, observes its natural outcome situation.
(3) guinea pig back divides 2 rows, and often arrange picture 3 diameters 1.5 centimetres of circles, the spacing of each circle is about about 1.5cm, and each circle represents 1 experimental group, avoids influencing each other between medicine.
(4) last to be coated with after bacterium 1 day, and medicine is outer in circle accordingly, medication every day 1 time.
(5) by Cavia porcellus respectively continuous use 4 days, 7 days each 10 at every turn, get skin in guinea pig back circle and carry out Drug efficacy evaluation.
6, the evaluation of curative effect of medication: often organize and put to death Cavia porcellus in 1 day after medication terminates respectively, its test block, back 75% cotton ball soaked in alcohol is sterilized, and cut each circular specimen with sterilization shears respectively, shred, be placed in the glass homogenizer that 2mlO.9% normal saline is housed, abundant homogenate, makes into suspension.Get 300ul suspension to add and have in the plate of solid medium, shake up immediately, make it be uniformly distributed in media surface, put 32 DEG C and cultivate and observe for 3 days.
(1) clump count in plate is observed.
(2) mycological cure rate: the clump count >1 in plate, regards it as and cultivates the positive.Calculate each group of mycology and cure number and fungus negative conversion rate.Mycology is cured: the direct microscopy of fungus and cultivation are feminine gender, fungus negative conversion rate: often organize mycology and cure number divided by often organizing Cavia porcellus sum.
6, statistical procedures:
Fungus feminine gender has statistical significance.All fungus colony numbers are means standard deviation, and application SPSS19.0 statistics analysis software package carries out data analysis.
Result:
Fungus colony number (see table 2) between each group after Drug therapy
Fungus colony number and fungus negative conversion rate between each group after table 2 Drug therapy
From table 2, at the 4th day of guinea pig skin chlosma infection place medication, statistical result showed, treatment group four sample CFU are obviously less than negative control group, blank group, there is significant difference (* P<0.01), compare between treatment group, treatment group fermentation composition D and treatment group fermentation composition B has statistical significance (#P<0.01).When medication the 7th day, treatment group four sample CFU are obviously less than negative control group, blank group, have significant difference (* P<0.01), treatment group fermentation composition B and other three treatment groups compare, and have significant difference.Medication is after 7 days, and the fungus negative conversion rate of fermentation composition D is 70%, and therapeutic effect is best.
Experimental example 3 is verified for anti-dandruff fermented tcm compositions shampoo trial effect
Choose squarrose 100 volunteers, use the shampoo containing fermented tcm compositions A prepared by embodiment 5, within every three days, use once, use 3 months continuously.The standard of curative effect on probation is: cure: after treatment, the dandruff all disappears; Effective: the dandruff obviously reduces, but do not disappear completely; Invalid: dandruff degree is not improved.Concrete result of use is in table 3.
The shampoo trial effect of table 3 containing fermented tcm compositions
The dandruff belongs to a kind of chronic disease of seborrheic dermatitis.Clinical manifestation skin lesion in the form of sheets, surface drying pityrosporum sample squama, pale asphyxia.Comb send out time or scratch scalp time have more pityrosporum sample squama to descend slowly and lightly.The fermented tcm compositions shampoo of the application of the invention, 100 volunteers, cure 45 examples, and effective 46 examples, no effect 9, effective percentage is 91%.
Claims (9)
1. for a fermented tcm compositions for shampoo, it is characterized in that, said composition forms compound recipe by sophora flavescens ait, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli, Cortex Phellodendri, Rhizoma Coptidis, Radix Scutellariae, obtains by the following method:
A. get Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli pulverizing, cross 40 mesh sieves, add the water of medical material weight 10 times, soak 1.0 hours, adjust pH is 7.0,115 DEG C of sterilizings 50 minutes;
B. when temperature is down to 33 DEG C, the bacillus cereus bacterium liquid that access preculture is good, inoculum concentration is volume ratio 3%, cultivates 20 ~ 24 hours, then accesses the good at least one strain above yeast bacterium liquid of preculture for 32 DEG C, every strain bacterium inoculum concentration is volume ratio 2%, cultivation temperature 30 DEG C, ferments 24 ~ 36 hours, is heated to 100 DEG C, and keep 10 minutes, centrifugally obtain supernatant;
Precipitation medicinal residues after centrifugal are added 85% ethanol of 10 times of weight, heating and refluxing extraction 1 hour, filter, decompression filtrate recycling ethanol, alcohol extract and the centrifugal supernatant obtained merge, and obtain four taste herb fermenting extracting solution; Or: the not alcohol extraction of precipitation medicinal residues, directly discards;
C. Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae soak 1 hour, decoct twice, each 1 hour, add the water of 10 times of medical material amounts for the first time, the water of second time 8 times of medical material amounts, and gradation filters, merging filtrate;
D. the four taste herb fermenting extracting solution filtrate of step C and step B obtained or supernatant merge, being evaporated to medical material weight g/ volume ml ratio is 20%, by medicine liquid heating to 80 DEG C, under stirring, slowly add the chitosan-acetic acid solution of 1% concentration, addition is 12% of medicine liquid volume; Sedimentation is after 8 ~ 12 hours, centrifugal, supernatant continue to be evaporated to medical material content be weight g/ volume ml than 50%, extracting solution precipitate with ethanol or not precipitate with ethanol, obtain fermented tcm compositions;
Described Traditional Chinese Medicine dosage is: Radix Sophorae Flavescentis 10-20 part, Flos Carthami 5-16 part, Fructus Cnidii 6-15 part, Fructus Zanthoxyli 5-16 part, Cortex Phellodendri 6-16 part, Rhizoma Coptidis 15-25 part, Radix Scutellariae 6-15g part.
2. the fermented tcm compositions for shampoo according to claim 1, it is characterized in that, described Traditional Chinese Medicine dosage is: Radix Sophorae Flavescentis 13 parts, 10 parts, Flos Carthami, Fructus Cnidii 12 parts, Fructus Zanthoxyli 16 parts, Cortex Phellodendri 10 parts, Rhizoma Coptidis 25 parts, Radix Scutellariae 14 parts.
3. the fermented tcm compositions for shampoo according to claim 1, is characterized in that, described bacillus cereus is Bacillus subtilis subspecies AS1.210, Bacillus subtilis natto; Described yeast is: reddish brown shadow yeast AS2.2114, Saccharomyces kluyveri AS2.4070, saccharomyces cerevisiae AS2.3852.
4. the fermented tcm compositions for shampoo according to claim 1, is characterized in that, described chitosan is the chitosan solution of 1% concentration prepared with the acetum of 1% concentration, and imbibition uses after spending the night.
5. the fermented tcm compositions for shampoo according to claim 1, it is characterized in that, the step of described precipitate with ethanol adds ethanol, make ethanol final concentration be 75%, sedimentation is spent the night, and filters, decompression recycling ethanol, it is that weight g/ volume ml is than 50% that residual liquid is settled to medical material content.
6., for a preparation method for the fermented tcm compositions of shampoo, it is characterized in that, step is as follows:
A. get Radix Sophorae Flavescentis, Flos Carthami, Fructus Cnidii, Fructus Zanthoxyli pulverizing, cross 40 mesh sieves, add the water of medical material weight 10 times, soak 1.0 hours, adjust pH is 7.0,115 DEG C of sterilizings 50 minutes;
B. when temperature is down to 33 DEG C, the bacillus cereus bacterium liquid that access preculture is good, inoculum concentration is volume ratio 3%, cultivates 20 ~ 24 hours, then accesses the good at least one strain above yeast bacterium liquid of preculture for 32 DEG C, every strain bacterium inoculum concentration is volume ratio 2%, cultivation temperature 30 DEG C, ferments 24 ~ 36 hours, is heated to 100 DEG C, and keep 10 minutes, centrifugally obtain supernatant;
Precipitation medicinal residues after centrifugal are added 85% ethanol of 10 times of weight, heating and refluxing extraction 1 hour, filter, decompression filtrate recycling ethanol, alcohol extract and the centrifugal supernatant obtained merge, and obtain four taste herb fermenting extracting solution; Or: the not alcohol extraction of precipitation medicinal residues, directly discards;
C. Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae soak 1 hour, decoct twice, each 1 hour, add the water of 10 times of medical material amounts for the first time, the water of second time 8 times of medical material amounts, and gradation filters, merging filtrate;
D. the four taste herb fermenting extracting solution filtrate of step C and step B obtained or supernatant merge, being evaporated to medical material weight g/ volume ml ratio is 20%, by medicine liquid heating to 80 DEG C, under stirring, slowly add the chitosan-acetic acid solution of 1% concentration, addition is 12% of medicine liquid volume; Sedimentation is after 8 ~ 12 hours, centrifugal, supernatant continue to be evaporated to medical material content be weight g/ volume ml than 50%, extracting solution precipitate with ethanol or not precipitate with ethanol, obtain fermented tcm compositions.
7. the fermented tcm compositions described in any one of claim 1-5 is preparing the application in shampoo.
8. application according to claim 7, is characterized in that, described shampoo is made up of shampoo substrate 95 parts, fermented tcm compositions 5 parts;
Described shampoo substrate is: 58.55 parts, water, laureth sulfuric ester ammonium 11 parts, lauryl alcohol sulfuric ester ammonium 7 parts, methyl hydroxybenzoate 0.1 part, propyl hydroxybenzoate 0.05 part, EDETATE SODIUM 0.1 part, coconut oleoyl amine MEA1.5 part, two (hydrogenated-tallow group) phthalic acid amide 0.5 part, glycol distearate 2 parts, guar hydroxypropyltrimonium chloride 0.3 part, VARISOFT TA100, cetrimonium chloride 0.4 part, piroctone olamine salt 0.7 part, citric acid 0.02 part, cocamido propyl betaine 4 parts, Polyethylene Glycol-14M0.06 part, glucose, glycerol polymethacrylates, dodecane hexadecyltrimethylammonium chloride 2 parts, pantothenylol 0.2 part, polyquaternary ammonium salt-70.5 parts, polydimethylsiloxane, cocamido propyl betaine, C12-15 Pareth-3, guar hydroxypropyltrimonium ammonium chloride 2 parts, dimethiconol, DBSA TEA salt 1.5, hyaluronate sodium 0.01 part, yeast extract 1 part, algae extract 1 part, 0.25 part, essence, ammonium chloride 0.2 part, magnesium nitrate, methylchloroisothiazandnone, Methylisothiazolinone 0.06 part.
9. application according to claim 8, is characterized in that, the preparation method of described shampoo is as follows:
A. successively the water of room temperature, laureth sulfuric ester ammonium, lauryl alcohol sulfuric ester ammonium are added manufacture tank, be stirred to raw material and all dissolve;
B. Polyethylene Glycol-14M, VARISOFT TA100, cetrimonium chloride, two (hydrogenated-tallow group) phthalic acid amide, methyl hydroxybenzoate, propyl hydroxybenzoate, EDETATE SODIUM is added successively, be stirred and heated to 80 DEG C, stop heating, be incubated 10 minutes, start cooling;
C. greenhouse cooling is to 70 DEG C, add coconut oleoyl amine MEA, glycol distearate successively, add after the water dispersed with stirring of guar hydroxypropyltrimonium chloride and 10 times, add piroctone olamine salt, dispersed with stirring is even, add citric acid, cocamido propyl betaine, glucose, glycerol polymethacrylates, dodecane hexadecyltrimethylammonium chloride, fermented tcm compositions, pantothenylol, polyquaternary ammonium salt-7, cool to 45 DEG C;
D. add other raw materials successively, stirring and dissolving is even, to obtain final product.
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