CN105527438A - Colloidal gold test strip for semi-quantitative detection of alpha-glucan, and detection method thereof - Google Patents
Colloidal gold test strip for semi-quantitative detection of alpha-glucan, and detection method thereof Download PDFInfo
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- CN105527438A CN105527438A CN201511003589.1A CN201511003589A CN105527438A CN 105527438 A CN105527438 A CN 105527438A CN 201511003589 A CN201511003589 A CN 201511003589A CN 105527438 A CN105527438 A CN 105527438A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
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- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
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- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/12—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar
- G01N2400/14—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar alpha-D-Glucans, i.e. having alpha 1,n (n=3,4,6) linkages between saccharide units, e.g. pullulan
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Abstract
The invention discloses a colloidal gold test strip for semi-quantitative detection of alpha-glucan, and a detection method thereof. The test strip comprises a baseboard, and a sample pad, a colloidal gold pad, a nitrocellulose membrane and a sample absorption pad which are sequentially connected on the baseboard. The nitrocellulose membrane is provided with a detection line antibody and a quality control line antibody, and the colloidal gold pad is provided with the colloidal gold antibody. The test strip is made according to a colloidal gold immunochromatography test (GICA) principle, and the alpha-glucan is detected by selecting an anti-alpha-glucan specific monoclonal antibody through adopting an antibody sandwich technology. The test strip has the advantages of strong specificity, high sensitivity, simple, convenient and fast operation, no need of special devices or professional trainings, clear and distinguishable result, economy and practicality in the alpha-glucan detection application process.
Description
Technical field
The present invention relates to a kind of colloidal gold strip and detection method of half-quantitative detection alpha-glucans.
Background technology
Alpha-glucans (dextran), also known as dextran, is the general name of the polymkeric substance of a class D-glucopyranose, forms main chain by α-1,6 glycosidic bond, and a small amount of branched fraction is then formed by α-1,2, α-1,3 and α-Isosorbide-5-Nitrae glycosidic bond.Molecular formula is (C
6h
10o
5)
n, molecular weight generally by tens thousand of to millions of, from solubility Small molecular to insoluble macromolecule, in glue-like, viscosity with molecular weight and concentration increase and increase.Alpha-glucans is white powder, odorless, tasteless, in usual water soluble, sucrose solution, sodium chloride solution, dissolubility with molecular weight and concentration increase and decline.Be insoluble to ethanol, ambient stable, heat variable color gradually or decomposition.After dilute acid hydrolysis, obtain part depolymerization product; If be hydrolyzed for a long time, glucose can be obtained.Dextran reacts through alkaline bleach liquor degradation, and the viscosity of product is significantly different with the length of hydrolysis time.Alpha-glucans is the microbial polysaccharide found the earliest, is the microbial polysaccharide of first suitability for industrialized production in the world, is also that the first of U.S. FDA approval can be used for the Microbial exopolysaccharides of food.
The alpha-glucans occurred in cane sugar manufacture production run, is commonly called as " sugarcane meal ".In fresh cane, the content of alpha-glucans is little, almost nil.But sugarcane as be subject to knife wound or weigh wounded, disease and pest, baked wheaten cake, frost or place long-time after harvesting, will be subject to Leuconostoc mesenteroides (
leuconostocmesnteroides), streptococcus (
streptococcus) etc. microorganism infection and form glucosan.These microorganisms under suitable temperature and humidity, can secrete dextransucrase (
dextransucrose), catalysing sucrose generates glucose and is polymerized and generates polysaccharide body, thus produces alpha-glucans.From sugarcane juice to purified product, sugar refining technology process also exists the harmful effect that alpha-glucans causes from start to finish.In sugarcane juice, the existence of alpha-glucans means that sugar transition loses, and viscosity increases, and clarifies, filters, evaporates, boils sugar (crystallization), purging is influenced, regains and reduces, and cost increases, and product quality declines, and affects the applicability of product.Therefore, the monitoring of alpha-glucans becomes an important technology of sugar refinery production control.
The general available Haze/ICUMSA method of the mensuration of alpha-glucans, Robert's copper method, PoticalActivityLtd.DASA method, enzymatic isolation method etc. in sugar industry, but these methods have larger limitation, as not high in accuracy, the shortcoming such as complex operation, inapplicable all sugar products.Wherein measure two main stream approach of alpha-glucans, HAZE method and Roberts method, or based on the deriving method of these two kinds of principles, all belong to chemical assay.These two kinds of method of testings all will use the characteristic that alpha-glucans precipitates in alcoholic solution.To there is reliability, accuracy, specificity in these method for quantitatively determining of alpha-glucans all not high in application for a long time, the shortcoming that expensive and complex operation is time-consuming.And high performance liquid chromatography, then there is instrument and equipment requirement high, the test duration is long, the problem of sample process difficulty.
Colloidal gold immunochromatographimethod technology, as a kind of detection technique, is characterized in convenient and swift, and without the need to any instrument and equipment except a small amount of reagent, a few minutes get final product visual results.The sample pre-treatments of colloidal gold strip is simple, and antijamming capability is strong, can meet and analyze requirement, be applicable to the quantitative and semi-quantitative primary dcreening operation of a large amount of sample.The colloidal gold strip of half-quantitative detection alpha-glucans of the present invention has the high sensitivity of ELISA method, significantly reduces testing cost.For convenient in sugar manufacturing industry, accurately the alpha-glucans detected in raw material, intermediate material, product and secondary product be highly significant.Result for retrieval shows, not yet has the method for half-quantitative detection alpha-glucans concentration, does not also utilize colloidal gold method to carry out the report of half-quantitative detection to alpha-glucans.
Summary of the invention
In order to overcome deficiency existing in prior art, the object of the present invention is to provide a kind of colloidal gold strip and detection method of half-quantitative detection alpha-glucans.
The technical solution used in the present invention is:
A kind of colloidal gold strip of half-quantitative detection alpha-glucans, comprise base plate, the sample pad that base plate is connected successively, gold mark pad, reaction film and suction sample pad, gold mark pad is coated with the monoclonal antibody of the anti-alpha-glucans of colloid gold label or the polyclonal antibody of anti-alpha-glucans; Described reaction film is positioned at gold mark pad one end and is provided with detection zone, quality control region is provided with away from gold mark pad one end, described detection zone is provided with 1 or many detection lines parallel to each other, detection line is coated with the monoclonal antibody of anti-alpha-glucans or the polyclonal antibody of anti-alpha-glucans.
Described quality control region is provided with nature controlling line, nature controlling line is coated with sheep anti-mouse antibody IgG.
The preparation method of the monoclonal antibody of described anti-alpha-glucans is:
1) alpha-glucans is mixed with albumen, use water-soluble solution, freeze drying, pulverize;
2) powder is placed in the constant temperature of 50 ~ 60 DEG C, reacts at least 3 days, obtain alpha-glucans-protein conjugate;
3) alpha-glucans-protein conjugate is mixed with adjuvant, emulsification, be expelled in animal body;
4) choose the animal body that serum titer is high, get its splenocyte and make suspension, and mix with the oncocyte of exponential phase, mediates fusion, screening obtain the hybridoma cell strain can secreting alpha-glucans monoclonal antibody specific;
5) use hybridoma to produce to obtain alpha-glucans monoclonal antibody specific or use ascites method to produce obtaining alpha-glucans monoclonal antibody specific.
As preferably, described albumen is the one in bovine serum albumin(BSA) or ovalbumin.
As preferably, the molecular weight of described alpha-glucans is at 10 ~ 2000kDa.
As preferably, the monoclonal antibody of described anti-alpha-glucans is secreted by hybridoma cell strain D9 and is obtained, and described hybridoma cell strain D9 is preserved in China typical culture collection center on Dec 7th, 2010, and deposit number is CCTCCNO:C2010107.
Detect a kit for alpha-glucans, comprise outter box and box inner support, it is characterized in that: described box inner support is provided with test strips groove and can places colloidal gold strip.
As preferably, described box inner support is also provided with some reagent pores, is placed with Reagent Tube in hole, in Reagent Tube, the reagent of splendid attire comprises alpha-glucans standard solution, sample diluting liquid respectively.
The principle of the colloidal gold strip of half-quantitative detection alpha-glucans of the present invention is: by the monoclonal antibody of the anti-alpha-glucans of colloid gold label, is coated in by this golden labeling antibody on gold mark pad.Detection line is coated with the monoclonal antibody of a certain amount of anti-alpha-glucans.After measuring samples being arrived gold mark pad, if containing alpha-glucans in sample, be then combined with the antibody of colloid gold label, form antigen-antibody colloid gold label compound.This compound swimming can the monoclonal antibody of anti-alpha-glucans of tested survey line be caught to during detection line, forms double-antibody sandwich compound, is gathered on detection line and develops the color.If alpha-glucans contained in sample is lower than certain amount, then after forming compound, swimming is very few to the double-antibody sandwich compound formed during detection line, can not develop the color at detection line.
By arranging the detection line at many intervals in test strips, the half-quantitative detection of alpha-glucans can be realized.When making this test strips, by limiting the antibody amount of each detection line wrapping quilt, make every bar detection line can in conjunction with a certain amount of antigen-antibody colloid gold label compound.From the near to the remote, during detection line colour developing, representative alpha-glucans content increases distance colloidal gold pad successively.According to the quantity occurring detection line in testing result, the concentration range of alpha-glucans in judgement sample.
Colloidal gold colloidal gold detection test paper strip high specificity of the present invention, highly sensitive, easy and simple to handle, can realize detecting fast alpha-glucans.
Accompanying drawing explanation
Fig. 1 is colloidal gold strip structural representation of the present invention (1 is sample pad, and 2 is gold mark pad, and 3 is reaction film, and 4 is detection line, and 5 is nature controlling line, and 6 for inhaling sample pad);
Fig. 2 is testing result schematic diagram (alpha-glucans concentration is less than 5ng/ml);
Fig. 3 is testing result schematic diagram (alpha-glucans concentration is 5-20ng/ml);
Fig. 4 is testing result schematic diagram (alpha-glucans concentration is 20-50ng/ml);
Fig. 5 is testing result schematic diagram (alpha-glucans concentration is greater than 50ng/ml).
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but be not limited thereto.
embodiment 1: the preparation method of alpha-glucans gold mark detection test paper
1. the preparation of collaurum:
Trisodium citrate reduction method is adopted to prepare collaurum.Get the chlorauric acid solution of 50ml0.1mg/ml, 100r/min stirs, and is heated to boiling, add 2ml10mg/ml sodium citrate solution, keep original temperature of reaction and stirring rate, continue boiling reaction 5min, after just having become limpid Chinese red to solution colour, stop reaction.Cooling, and be placed in brown bottle 4 DEG C and keep in Dark Place.Detect through Electronic Speculum, collaurum particle diameter is 10-15nm.
2. the preparation of alpha-glucans-BSA cross-linking agent
By alpha-glucans DextranT-40 and BSA in the mixing of 1:1 ratio, be demodulated to 6% (W/V), freeze drying with water-soluble.Place in 60 DEG C of environment after being ground into powder, and place saturated KBr solution reaction 7 days at container bottom.Every day, SDS-PAGE electrophoresis was carried out in sampling, and the dyeing of periodic acid-Schiff reagent method and Coomassie brilliant blue R250 dye and identify.
3. the preparation of monoclonal antibody
With the cross-linking agent immune mouse of alpha-glucans DextranT-40 and BSA, splenocyte and the small white mouse myeloma cell hybrid fusion of getting immune mouse prepare hybridoma, indirect ELISA method screening positive clone, adopt limiting dilution assay to carry out subclone and the cultivation of hybridoma, screening can the cell line of stably excreting anti alpha-dextran monoclonal antibody simultaneously.Wherein a strain of hybridoma strain D9 is preserved in China typical culture collection center (CHINACENTERFORTYPECULTURECOLLECTION, CCTCC) on Dec 7th, 2010; Address: China, Wuhan, Wuhan University; Deposit number is CCTCCNO:C2010107.
By being inoculated into the abdominal cavity of BALB/C mice after hybridoma cell strain D9 in vitro culture to certain density, collecting induced ascites, obtaining anti alpha-dextran monoclonal antibody by affinity chromatography method.
4. the preparation of colloid gold label thing
Get 100ml colloidal gold solution 0.1mol/L solution of potassium carbonate and be adjusted to pH8.0, add the monoclonal antibody of the anti-alpha-glucans of 0.6mg while stirring, stirring at room temperature 60min. dropwise adds the bovine serum albumin(BSA) (BSA) of 5% again, its final concentration is made to be 1%, 1h is left standstill after stirring, 2000r/min, 4 DEG C of low-speed centrifugal 30min, get supernatant.By supernatant obtained in the previous step in 12000r/min, 4 DEG C of highly centrifugal 15min, abandon and ask, collecting precipitation.With PBS damping fluid (containing the 1%BSA) dissolution precipitation of 0.01M, pH7.2, be placed in 4 DEG C for subsequent use.
5. the preparation of colloidal gold pad
Collaurum-anti alpha-dextran monoclonal antibody compound uses 0.1mol/L potassium carbonate buffer to be diluted to setting concentration, and even application is on glass fibre element film.Colloidal gold pad there is 0.10 ~ 0.50ug/cm
2colloidal gold labeled monoclonal antibody, the alpha-glucans content in the golden labeling antibody in this concentration range and conventional sense situation in sample matches.37 DEG C of dry 30min obtain colloidal gold pad.
6. detect the preparation of diaphragm
With the PBS damping fluid of 0.01M, pH7.2, the monoclonal antibody of anti-alpha-glucans is diluted to three variable concentrations, every bar line sprays 20ul, live width 1mm respectively, distance between centers of tracks 5mm, its package amount represents and can detect 5ng/ml, the amount of the alpha-glucans of 20/ml, 50ng/ml.Form detection line (T line), vacuum drying.Be sprayed on nitrocellulose filter by the sheep anti-mouse antibody IgG of 0.5mg/mL, concentration is 0.5ul/cm, and live width is 1mm, as nature controlling line (C line), closes, vacuum drying with 1%BSA, 4 DEG C of preservations.
7. the assembling of test strips
Sample pad (glass fibre membrane), colloidal gold pad, nitrocellulose filter and suction sample pad order is pasted onto on PVC base plate successively.Nitrocellulose filter is positioned in the middle part of base plate, nitrocellulose filter one end in detection line region and the connection of colloidal gold pad, and the one end in control line region is connected with suction sample pad, and sample pad and colloidal gold pad overlap.Be cut into the test strips that width is 5mm, also test strips can be loaded in a plastic clip and make test card.The sealing aluminium foil bag put into drying agent saves backup.
embodiment 2: the using method of test strip of the present invention
Sample thief 100ul is added drop-wise to sample pad, starts timing, observes testing result after 15min.
The sample concentration representated by three detection lines designed in this test strips is 5ng/ml, 20ng/ml, 50ng/ml, and acquired results interpretation standard is as follows:
1., when nature controlling line is without display (Fig. 1), test result is invalid.
2., when there is nature controlling line, without (Fig. 2) during detection line, recording alpha-glucans concentration and being less than 5ng/ml.
3., when occurring nature controlling line and a detection line (Fig. 3), recording alpha-glucans concentration is 5-20ng/ml.
4., when occurring nature controlling line and two detection lines (Fig. 4), recording alpha-glucans concentration is 20-50ng/ml.
5., when occurring nature controlling line and three detection lines (Fig. 5), recording alpha-glucans concentration and being greater than 50ng/ml.In this case measure again after sample suitably can being diluted.
embodiment 3: sample detection example
Adopt the method for embodiment 2 to detect all kinds of sample, testing result is as shown in table 1:
the testing result of all kinds of sample of table 1
Wherein the test result of DextranT2000 and DextranT40 standard solution meets expection.Glucose, starch, the samples such as beta glucan can not cause interference to detection, demonstrate test strips and have good specificity.
Above embodiment shows, alpha-glucans colloidal gold strip high specificity of the present invention, highly sensitive, simple, convenient, quick, and do not need special instruments and equipment and professional training, result is distinct easily to be distinguished, economical and practical.
Above embodiment is only introduces preferred case of the present invention, to those skilled in the art, not deviating from any apparent changes and improvements of carrying out in the scope of spirit of the present invention, all should be regarded as a part of the present invention.
Claims (8)
1. the colloidal gold strip of a half-quantitative detection alpha-glucans, comprise base plate, the sample pad that base plate is connected successively, gold mark pad, reaction film and suction sample pad, gold mark pad is coated with the monoclonal antibody of the anti-alpha-glucans of colloid gold label or the polyclonal antibody of anti-alpha-glucans; Described reaction film is positioned at gold mark pad one end and is provided with detection zone, quality control region is provided with away from gold mark pad one end, described detection zone is provided with 1 or many detection lines parallel to each other, detection line is coated with the monoclonal antibody of anti-alpha-glucans or the polyclonal antibody of anti-alpha-glucans.
2. colloidal gold strip according to claim 1, is characterized in that, described quality control region is provided with nature controlling line, nature controlling line is coated with sheep anti-mouse antibody IgG.
3. colloidal gold strip according to claim 1, is characterized in that, the preparation method of the monoclonal antibody of described anti-alpha-glucans is:
1) alpha-glucans is mixed with albumen, use water-soluble solution, freeze drying, pulverize;
2) powder is placed in the constant temperature of 50 ~ 60 DEG C, reacts at least 3 days, obtain alpha-glucans-protein conjugate;
3) alpha-glucans-protein conjugate is mixed with adjuvant, emulsification, be expelled in animal body;
4) choose the animal body that serum titer is high, get its splenocyte and make suspension, and mix with the oncocyte of exponential phase, mediates fusion, screening obtain the hybridoma cell strain can secreting alpha-glucans monoclonal antibody specific;
5) use hybridoma to produce to obtain alpha-glucans monoclonal antibody specific or use ascites method to produce obtaining alpha-glucans monoclonal antibody specific.
4. colloidal gold strip according to claim 3, is characterized in that, described albumen is the one in bovine serum albumin(BSA) or ovalbumin.
5. colloidal gold strip according to claim 3, is characterized in that, the molecular weight of described alpha-glucans is at 10 ~ 2000kDa.
6. colloidal gold strip according to claim 1, it is characterized in that, the monoclonal antibody of described anti-alpha-glucans is secreted by hybridoma cell strain D9 and is obtained, described hybridoma cell strain D9 is preserved in China typical culture collection center on Dec 7th, 2010, and deposit number is CCTCCNO:C2010107.
7. detect a kit for alpha-glucans, comprise outter box and box inner support, it is characterized in that: described box inner support is provided with test strips groove and can places the arbitrary described colloidal gold strip of claim 1-6.
8. the kit of detection alpha-glucans according to claim 7, it is characterized in that: described box inner support is also provided with some reagent pores, be placed with Reagent Tube in hole, in Reagent Tube, the reagent of splendid attire comprises alpha-glucans standard solution, sample diluting liquid respectively.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105929175A (en) * | 2016-05-03 | 2016-09-07 | 长春圣金诺生物制药有限公司 | Semi-quantitative detection colloidal gold test paper for cystatin C in urine |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1547024A (en) * | 2003-12-03 | 2004-11-17 | 华南农业大学 | Fast and semiquantitative detection method for Clenbuterol |
| CN1811446A (en) * | 2005-06-20 | 2006-08-02 | 华南农业大学 | Streptomycin immune chromatographic semiquantitative test paper detecting method |
| CN101852800A (en) * | 2010-05-18 | 2010-10-06 | 同昕生物技术（北京）有限公司 | Colloidal gold test strip for semi-quantitatively detecting concentration of tacrolimus drug in human whole blood and detection method |
| CN101915833A (en) * | 2010-09-10 | 2010-12-15 | 中国科学院东北地理与农业生态研究所 | Semi-quantitative rapid detection test paper for immunoglobulin G in bovine colostrum and products thereof and preparation method thereof |
| CN102127143A (en) * | 2010-12-27 | 2011-07-20 | 广州甘蔗糖业研究所 | Preparation method of alpha-glucan antigen and specificity monoclonal antibody |
| CN102183663A (en) * | 2011-03-24 | 2011-09-14 | 武汉璟泓万方堂医药科技有限公司 | Qualitative semi-quantitative dual-purpose female ovulation hormone detection test paper and colorimetric card |
| CN102353787A (en) * | 2011-09-27 | 2012-02-15 | 山东莱博生物科技有限公司 | Colloidal gold test paper strip for semiquantitatively detecting concentration of GFAP (Glial Fibrillary Acidic Protein) in human serum |
| CN202502097U (en) * | 2012-01-16 | 2012-10-24 | 中国计量学院 | Enzyme linked immuno-sorbent assay board and kit for rapidly detecting specificity radiolysis product DiHT in irradiated foods |
| CN103698520A (en) * | 2013-11-28 | 2014-04-02 | 广州甘蔗糖业研究所 | Kit for quantitatively detecting alpha-glucan by monoclonal antibody immunonephelometry |
| CN104535768A (en) * | 2014-11-25 | 2015-04-22 | 东莞市青麦田数码科技有限公司 | Urinary microalbumin detection apparatus |
-
2015
- 2015-12-25 CN CN201511003589.1A patent/CN105527438A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1547024A (en) * | 2003-12-03 | 2004-11-17 | 华南农业大学 | Fast and semiquantitative detection method for Clenbuterol |
| CN1811446A (en) * | 2005-06-20 | 2006-08-02 | 华南农业大学 | Streptomycin immune chromatographic semiquantitative test paper detecting method |
| CN101852800A (en) * | 2010-05-18 | 2010-10-06 | 同昕生物技术（北京）有限公司 | Colloidal gold test strip for semi-quantitatively detecting concentration of tacrolimus drug in human whole blood and detection method |
| CN101915833A (en) * | 2010-09-10 | 2010-12-15 | 中国科学院东北地理与农业生态研究所 | Semi-quantitative rapid detection test paper for immunoglobulin G in bovine colostrum and products thereof and preparation method thereof |
| CN102127143A (en) * | 2010-12-27 | 2011-07-20 | 广州甘蔗糖业研究所 | Preparation method of alpha-glucan antigen and specificity monoclonal antibody |
| CN102183663A (en) * | 2011-03-24 | 2011-09-14 | 武汉璟泓万方堂医药科技有限公司 | Qualitative semi-quantitative dual-purpose female ovulation hormone detection test paper and colorimetric card |
| CN102353787A (en) * | 2011-09-27 | 2012-02-15 | 山东莱博生物科技有限公司 | Colloidal gold test paper strip for semiquantitatively detecting concentration of GFAP (Glial Fibrillary Acidic Protein) in human serum |
| CN202502097U (en) * | 2012-01-16 | 2012-10-24 | 中国计量学院 | Enzyme linked immuno-sorbent assay board and kit for rapidly detecting specificity radiolysis product DiHT in irradiated foods |
| CN103698520A (en) * | 2013-11-28 | 2014-04-02 | 广州甘蔗糖业研究所 | Kit for quantitatively detecting alpha-glucan by monoclonal antibody immunonephelometry |
| CN104535768A (en) * | 2014-11-25 | 2015-04-22 | 东莞市青麦田数码科技有限公司 | Urinary microalbumin detection apparatus |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105929175A (en) * | 2016-05-03 | 2016-09-07 | 长春圣金诺生物制药有限公司 | Semi-quantitative detection colloidal gold test paper for cystatin C in urine |
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