CN102183663A - Qualitative semi-quantitative dual-purpose female ovulation hormone detection test paper and colorimetric card - Google Patents
Qualitative semi-quantitative dual-purpose female ovulation hormone detection test paper and colorimetric card Download PDFInfo
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- CN102183663A CN102183663A CN2011100722409A CN201110072240A CN102183663A CN 102183663 A CN102183663 A CN 102183663A CN 2011100722409 A CN2011100722409 A CN 2011100722409A CN 201110072240 A CN201110072240 A CN 201110072240A CN 102183663 A CN102183663 A CN 102183663A
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Abstract
The invention discloses a test paper for qualitative semi-quantitative dual-purpose detection of female ovulation, which is formed mainly by overlapping and connecting a bottom plate, a nitrocellulose membrane, an absorption pad, a polyester film and a glass fiber membrane mutually, wherein the middle of the bottom plate is adhered with the nitrocellulose membrane on which two antibody lines are engraved, one line is a detection line coated by a first anti-LH monoclonal antibody, and the other line is a quality control line coated by goat anti mouse IgG monoclonal antibody; and the upper edge position of the nitrocellulose membrane is adhered with the water absorption pad, the lower edge position of the nitrocellulose membrane is adhered with a combined pad which combines another anti-LH monoclonal antibody of marking colloidal gold or color rubber latex, and the lower edge position of the combined pad is adhered with a sample pad. A woman can use the test paper of the invention at home to qualitatively and semi-quantitatively detect the content of LH luteinizing hormone of the urine or blood of the woman, and knows the ovulation date, or knows whether hormonal infertility exists or not.
Description
Technical field
The present invention relates to a kind of test paper of half-quantitative detection women's egg laying hormone level and use this detection paper ovulation, determine the ovulation date or detect the infertile method of hormonal.
Background technology
Interstitialcellstimulating hormone (ICSH) (LH) is secreted by the adenohypophysis basophil.At the collaborative FSH(follicle-stimulating hormone (FSH) of women (LH)) acting in conjunction keeps the menstrual cycle of ovary, causes ovulation and corpus luteum to form.The generation of LH is subjected to the control of hypothalamus gonadotropin releasing hormone, regulated and control by the positive-negative feedback of ovary.Impel ovulation under the FSH synergy, form corpus luteum justacrine progestational hormone, can predicting ovulation.When follicle maturity, secrete a large amount of estrogen, make hypophysis discharge a large amount of interstitialcellstimulating hormone (ICSH)s, form the interstitialcellstimulating hormone (ICSH) peak, a large amount of interstitialcellstimulating hormone (ICSH)s can make ripe follicular rupture, ovulation.
Release peak and ovary ovulation at menstrual cycle LH have substantial connection, the LH peak indicates ovary ovulation in 1-2 days once appearance, therefore can monitor LH peak value in serum or the urine in the menstrual cycle, become pregnant the time with definite the best, maybe need to take the time of contraceptives.This testing result is to represent greater than 25 milli-international unit/milliliters (MIU/ML).
The present invention can provide with giving birth to become pregnant the authentic communication that is closely related, user's breeding time in needs provide physiological period initial sufficient predict data, thereby can the prediction love life gestation or need do not taked contraceptives.Or can the hormonal readiness that detect the women reach pregnant level.If hormonal readiness is low excessively, as be lower than 5 miu/ml, illustrate that then the women has owing to hormonal readiness is crossed the low infertility of suffering from.
Domestic and foreign hospitals utilizes colloidal gold immune chromatography test to detect the luteinizing principle level at present, mainly utilizes the collaurum qualitative detection to be the master.Testing result can only be judged having or not of hormonal readiness, just, and can not judge the concrete content of hormonal readiness, and in the check of concrete clinical practice, determine that the concrete content of hormonal readiness has more reference value.
Domestic and foreign hospitals all is to use the LH radioimmunological kit at present, the LH enzyme is exempted from kit and qualitative colloid gold test paper, this has needs the professional of hospital, professional equipment could detect, and the time that needs is long also very complicated, can not be used for family and test oneself, what have can only be qualitative, can not play the directiveness effect.
The CN00112779.9 Patent publish that the Kunming cloud is big a kind of infertility detect test paper and detection method thereof, it has only an antibody line on nitrocellulose membrane, do not have nature controlling line, when practical operation, easily judge by accident infertile because of the inefficacy of test strips or hormonal readiness low.
The Patent publish of the CN200610200333 of Gansu Lanzhou University a kind of tachysynthesis diagnosis test paper of colloidal gold semi-quantitative method, detection line with quantitatively arrange about line, on same horizontal line, middlely add a narrow linear slit again, be not easy to mass production.
Summary of the invention
Whether technical matters to be solved by this invention is the shortcoming that overcomes above-mentioned technology, provide a kind of women just can finish detection at home voluntarily and ovulate, and can the oneself detect the specifically dual-purpose detection test paper of qualitative semi of ovulation LH hormonal readiness.
For solving the problems of the technologies described above, the test paper of the dual-purpose detection women ovulation of qualitative semi of the present invention, main base plate, nitrocellulose filter by PVC or PS material, adsorptive pads, polyester film, glass fibre membrane overlap mutually and are formed by connecting, and it is characterized in that, nitrocellulose membrane is glued at the middle part of described base plate, drawing on the nitrocellulose membrane has two antibody lines, and one is the detection line of an anti-LH monoclonal antibody bag quilt, and another is the nature controlling line of sheep anti-mouse igg antibody sandwich; Upper edge at nitrocellulose membrane is glued adsorptive pads along the position, the sticking pad that combines another anti-LH monoclonal antibody of mark collaurum or color latex in nitrocellulose membrane lower edges position, and sample pad is glued in the lower edges position of pad.
The adsorptive pads on described nitrocellulose membrane and top, the pad of nitrocellulose membrane and lower end, the overlapped 1--4mm of the part that joins between the sample pad of pad and bottom.
The gold grain of described collaurum or the anti-LH monoclonal antibody of color latex mark or latex size are between 20nm--120nm.
As preferred version, to sheep anti-mouse igg antibody concentration in the nature controlling line, anti-LH monoclonal anti bulk concentration in the detection line, gold mark LH monoclonal anti bulk concentration is adjusted, and makes when test sample, when the LH hormone concentration was 25 Miu/ml in the sample, nature controlling line was identical with the detection line red color depth.When the LH hormone concentration was lower than 25miu/ml in the sample, the redness of detection line was shallower than the redness of nature controlling line, and when the LH hormone concentration was higher than 25MIU/ml in the sample, the redness of detection line was deeper than the redness of nature controlling line.When hormone concentration is lower than 5miu/ml in the sample, the nature controlling line redness, and detection line is colourless.
The colorimetric card that the test paper of the dual-purpose detection women ovulation of a kind of and qualitative semi is used contains the red piece of multiple red gradient in the colorimetric card, the red piece of every kind of depth detects the shown redness of variable concentrations LH Detection of antigen to using ovulation test paper.By the contrast ratio colour atla, can find out the concentration of the LH in the test sample.
Detection principle of the present invention is: urine or droplet of blood 4-5 are dripped on the sample pad, or the lower end of sample pad is dipped in urine or the blood, LH antigen in urine or the blood is by the capillary action of sample pad, move to the nitrocellulose membrane direction, during through gold-marking binding pad, combine with the anti-LH monoclonal antibody specificity on the gold-marking binding pad, and bond is moved together to nitrocellulose membrane, when on arriving nitrocellulose membrane, being coated with the detection line of anti-LH monoclonal antibody, the combination of double antibodies sandwich specificity takes place, the anti-LH monoclonal antibody of antigen in the sample and golden target is coated on the anti-LH monoclonal antibody of nitrocellulose membrane and fixes, thereby make detection line show redness, the red depth of its detection line and the LH antigen concentration journey direct ratio in the sample of collaurum.Gold mark LH monoclonal antibody that not tested survey line is fixing and LH hormone antigen bond or gold mark LH monoclonal antibody continue along moving on the nitrocellulose membrane, when reaching nature controlling line, gold mark LH monoclonal antibody will be by the sheep anti-mouse igg antibody on the nature controlling line in conjunction with fixing, thereby make nature controlling line, show redly, no matter whether contain LH antigen in the sample, nature controlling line develops the color all the time, if nature controlling line does not develop the color, illustrate that test strips is invalid.
The present invention adopts the colloidal gold immunochromatographimethod single stage method, but not only can be qualitative but half-quantitative detection women urine or blood in the short corpus luteum of LH produce hormone-content, the women uses test paper of the present invention at home, just knows LH hormone secretion situation in the body, can predict oneself ovulation date.Whether opportunity or selection need to take contraceptives thereby decision is become pregnant.SF also can detect and understand oneself whether to suffer from the hormonal infertility by test strips of the present invention.
Description of drawings
Below in conjunction with the drawings and specific embodiments technical scheme of the present invention is further described in detail.
Fig. 1 is the front view of the dual-purpose detection women of qualitative semi egg laying hormone test paper.
Fig. 2 is the side view of the dual-purpose detection women of qualitative semi egg laying hormone test paper.
The colorimetric card front view of Fig. 3 for cooperating the dual-purpose detection women of qualitative semi egg laying hormone test paper to use.
Embodiment
Shown in Fig. 1,2, the dual-purpose detection women of qualitative semi egg laying hormone test paper is mainly by the sample pad 01 of glass fibre membrane material, the pad 02 of polyester film or glass fibre membrane material, adsorptive pads 06, base plate 07, nitrocellulose membrane 04 5 parts are formed, draw two parallel antibody capture lines on the nitrocellulose membrane 04 respectively, an anti-LH monoclonal antibody near pad, as detection line 03, another contains through having determined the sheep anti-mouse igg antibody of concentration, as normal concentration colour developing control line 05, be also referred to as nature controlling line, two lines are at a distance of 4--7mm; Be sprayed with another anti-LH monoclonal antibody of collaurum or color latex mark on the pad 02.The adsorptive pads 06 on nitrocellulose membrane 04 and top, the pad 02 of nitrocellulose membrane 04 and lower end, the handing-over of overlapping respectively between the sample pad 06 of pad 02 and bottom, overlapping width is respectively 1-4mm.The gold grain of the anti-LH monoclonal antibody of another of used collaurum or color latex mark or latex size are between 20nm--120nm.
To sheep anti-mouse igg antibody concentration in the nature controlling line, anti-LH monoclonal anti bulk concentration in the detection line, gold mark LH monoclonal anti bulk concentration, adjust, make when test sample, when the LH hormone concentration was 25 Miu/ml in the sample, nature controlling line was identical with the detection line red color depth.When the LH hormone concentration was lower than 25miu/ml in the sample, the redness of detection line was shallower than the redness of nature controlling line, and when the LH hormone concentration was higher than 25MIU/ml in the sample, the redness of detection line was deeper than the redness of nature controlling line.When hormone concentration is lower than 5miu/ml in the sample, the nature controlling line redness, and detection line is colourless.
Using method of the present invention is: the selected time that begins to detect, 1 week beginning after the women of the detection selection for the first time menstruation.In the relatively-stationary time of every day, as morning etc., get a test paper, urine or droplet of blood 4-5 are dripped on the sample pad, or the lower end of sample pad is dipped in urine or the blood, in 5 minutes, nature controlling line should show redness; If nature controlling line does not develop the color, illustrate that this test paper is invalid.Observe detection line, and the color and the nature controlling line of detection line contrasted the red depth of detection line and the LH antigen concentration journey direct ratio in the sample.The contrast colour atla, the result of record detection every day.Continuous detecting 10 days was a sense cycle.
If during certain in one-period detects, detection line is red to be deepened suddenly, and surpasses nature controlling line, illustrates that then the women is about to ovulation in two days.These two days will be the preferably opportunity of becoming pregnant, if do not want to become pregnant, then need to take corresponding contraceptives.
If the women is in a sense cycle, the detection line redness is lower than the colour of colour atla 10miu/ml always, illustrates that then the LH hormonal readiness is low excessively in detected person's body, may suffer from the infertility that hypobasophilism causes.
If in the sense cycle, the detection line redness is lower than the redness of nature controlling line always, illustrate that then the LH hormonal readiness is lower than 25miu/ml in detected person's body, may suffer from infertility because of can not normally ovulating and cause.
If in a sense cycle, the detection line redness is higher than the nature controlling line redness always, and greater than colour atla 60miu/ml colour, illustrate that then the LH hormonal readiness is too high in detected person's body, hormone secretion is unusual, may suffer from the disease that certain needs treatment and cure badly.
The test paper of the dual-purpose detection women ovulation of the qualitative semi of the present invention preparation and with the ELISA kit, the pregnant woman's urina sanguinis sample made a definite diagnosis and the 50 negative urine samples of choosing six tame hospitals in 200 parts of Wuhan City are detected, the result is as follows:
The test paper of the dual-purpose detection women ovulation of qualitative semi: 200 parts of positives, 50 feminine genders.
ELISA kit: 200 parts of positives, 50 parts of feminine genders.
The detection paper result and the total coincidence rate of ELISA kit testing result of the dual-purpose detection women ovulation of qualitative semi are 100%.
It should be noted last that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (5)
1. the dual-purpose detection women of qualitative semi egg laying hormone test paper, mainly by base plate, nitrocellulose filter, adsorptive pads, polyester film, glass fibre membrane overlaps mutually and is formed by connecting, it is characterized in that nitrocellulose membrane is glued at the middle part of described base plate, drawing on the nitrocellulose membrane has two antibody lines, article one, be the detection line of an anti-LH monoclonal antibody bag quilt, another is the nature controlling line of sheep anti-mouse igg monoclonal antibody bag quilt; Upper edge at nitrocellulose membrane is glued adsorptive pads along the position, the sticking pad that combines another anti-LH monoclonal antibody of mark collaurum or color latex in nitrocellulose membrane lower edges position, and sample pad is glued in the lower edges position of pad.
2. the dual-purpose detection women of qualitative semi according to claim 1 egg laying hormone test paper, it is characterized in that, the adsorptive pads on described nitrocellulose membrane and top, the pad of nitrocellulose membrane and lower end, the overlapped 1--4mm of the part that joins between the sample pad of pad and bottom.
3. the dual-purpose detection women of qualitative semi according to claim 1 and 2 egg laying hormone test paper is characterized in that the gold grain of described collaurum or the anti-LH monoclonal antibody of color latex mark or latex size are between 20nm--120nm.
4. the dual-purpose detection women of qualitative semi according to claim 3 egg laying hormone test paper, it is characterized in that, anti-LH monoclonal anti bulk concentration in sheep anti-mouse igg antibody concentration in the nature controlling line, the detection line and gold mark LH monoclonal anti bulk concentration are adjusted, make when test sample, when the LH hormone concentration was 25 Miu/ml in the sample, nature controlling line was identical with the detection line red color depth; When the LH hormone concentration was lower than 25miu/ml in the sample, the redness of detection line was shallower than the redness of nature controlling line, and when the LH hormone concentration was higher than 25MIU/ml in the sample, the redness of detection line was deeper than the redness of nature controlling line; When hormone concentration is lower than 5miu/ml in the sample, the nature controlling line redness, and detection line is colourless.
5. colorimetric card that cooperates the dual-purpose detection women of the described qualitative semi of claim 4 egg laying hormone detection paper to use, it is characterized in that, the red piece that contains multiple red gradient in the described colorimetric card, the red piece of every kind of depth detects the shown redness of variable concentrations LH Detection of antigen to using ovulation test paper.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102565426A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing luteinizing hormone (LH) in semi-quantitative mode by employing double-indicatrix immunochromatography |
| CN103163304A (en) * | 2011-12-19 | 2013-06-19 | 天津中新科炬生物制药有限公司 | Method for rapidly detecting follicle stimulating hormone (FSH) in urine sample in a quantitative mode |
| CN103185804A (en) * | 2011-12-28 | 2013-07-03 | 丁鑫 | Method for semiquantitatively measuring LH concentration by combination of ovulation test strips of different sensitivities |
| CN104020286A (en) * | 2014-06-10 | 2014-09-03 | 武汉璟泓万方堂医药科技股份有限公司 | Quantitative detection instrument and detection method of immunochromatography test strip |
| CN105527438A (en) * | 2015-12-25 | 2016-04-27 | 广州甘蔗糖业研究所 | Colloidal gold test strip for semi-quantitative detection of alpha-glucan, and detection method thereof |
| CN105628917A (en) * | 2015-12-25 | 2016-06-01 | 广州甘蔗糖业研究所 | Gold-colloid test strip for detecting alpha-glucosan and detection method |
| CN106443022A (en) * | 2016-07-12 | 2017-02-22 | 昆明云大生物技术有限公司 | Luteinizing hormone semi-quantitative ovulation determination test paper and making method thereof |
| CN106771272A (en) * | 2016-12-07 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection method of Furacilin metabolite and detection card in a kind of animal tissue |
| CN107688090A (en) * | 2017-07-17 | 2018-02-13 | 润和生物医药科技(汕头)有限公司 | A kind of NTx Test papers, kit and preparation method thereof |
| CN109406773A (en) * | 2018-12-27 | 2019-03-01 | 正元盛邦(天津)生物科技有限公司 | A kind of capillary detection kit and preparation method thereof for interstitialcellstimulating hormone (ICSH) |
| CN109887044A (en) * | 2019-03-21 | 2019-06-14 | 北京大学第一医院 | A kind of reproductive data assessment method and system |
| CN111562397A (en) * | 2020-06-04 | 2020-08-21 | 昆明天沃生物科技有限公司 | Method for detecting mating opportunity of cattle |
| CN111562396A (en) * | 2020-06-04 | 2020-08-21 | 昆明天沃生物科技有限公司 | Method for detecting hybridization opportunity of sheep |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103163304A (en) * | 2011-12-19 | 2013-06-19 | 天津中新科炬生物制药有限公司 | Method for rapidly detecting follicle stimulating hormone (FSH) in urine sample in a quantitative mode |
| CN102565426A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing luteinizing hormone (LH) in semi-quantitative mode by employing double-indicatrix immunochromatography |
| CN103185804A (en) * | 2011-12-28 | 2013-07-03 | 丁鑫 | Method for semiquantitatively measuring LH concentration by combination of ovulation test strips of different sensitivities |
| CN104020286B (en) * | 2014-06-10 | 2016-08-31 | 武汉璟泓万方堂医药科技股份有限公司 | A kind of immuno-chromatographic test paper strip instrument and detection method |
| CN104020286A (en) * | 2014-06-10 | 2014-09-03 | 武汉璟泓万方堂医药科技股份有限公司 | Quantitative detection instrument and detection method of immunochromatography test strip |
| CN105527438A (en) * | 2015-12-25 | 2016-04-27 | 广州甘蔗糖业研究所 | Colloidal gold test strip for semi-quantitative detection of alpha-glucan, and detection method thereof |
| CN105628917A (en) * | 2015-12-25 | 2016-06-01 | 广州甘蔗糖业研究所 | Gold-colloid test strip for detecting alpha-glucosan and detection method |
| CN106443022A (en) * | 2016-07-12 | 2017-02-22 | 昆明云大生物技术有限公司 | Luteinizing hormone semi-quantitative ovulation determination test paper and making method thereof |
| CN106771272A (en) * | 2016-12-07 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection method of Furacilin metabolite and detection card in a kind of animal tissue |
| CN107688090A (en) * | 2017-07-17 | 2018-02-13 | 润和生物医药科技(汕头)有限公司 | A kind of NTx Test papers, kit and preparation method thereof |
| CN109406773A (en) * | 2018-12-27 | 2019-03-01 | 正元盛邦(天津)生物科技有限公司 | A kind of capillary detection kit and preparation method thereof for interstitialcellstimulating hormone (ICSH) |
| CN109887044A (en) * | 2019-03-21 | 2019-06-14 | 北京大学第一医院 | A kind of reproductive data assessment method and system |
| CN109887044B (en) * | 2019-03-21 | 2022-02-11 | 北京大学第一医院 | Reproductive data evaluation method and system |
| CN111562397A (en) * | 2020-06-04 | 2020-08-21 | 昆明天沃生物科技有限公司 | Method for detecting mating opportunity of cattle |
| CN111562396A (en) * | 2020-06-04 | 2020-08-21 | 昆明天沃生物科技有限公司 | Method for detecting hybridization opportunity of sheep |
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Application publication date: 20110914 |