CN105510109A - Blood sample sampling pretreatment device and method - Google Patents
Blood sample sampling pretreatment device and method Download PDFInfo
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- CN105510109A CN105510109A CN201510867804.6A CN201510867804A CN105510109A CN 105510109 A CN105510109 A CN 105510109A CN 201510867804 A CN201510867804 A CN 201510867804A CN 105510109 A CN105510109 A CN 105510109A
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- suction pipe
- blood sample
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- pretreatment device
- blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a blood sample sampling pretreatment device and method. The device comprises a blood sampling part and a sample mixing part which are detachably connected, wherein the blood sampling part comprises a suction pipe and an elastomer; a compressing opening is formed in an elastic wall; a sealing ring is arranged at the joint of the compressing opening and the suction pipe; bonding layers for sealing the joint of the compressing opening and the suction pipe are arranged on the two sides of the sealing ring; the sample mixing part comprises a mixing container for accommodating the suction pipe; a sealing film that the end part of the suction pipe can puncture is arranged at the lower half part of the mixing container; a sealed treating liquid accommodating layer is formed by the sealing film and the container wall of the mixing container; a liquid discharging unit communicated with the treating liquid accommodating layer is also arranged at the bottom of the mixing container; a container opening corresponding to the suction pipe in pipe diameter size is formed in the top of the mixing container, so that the elastomer can be fixed on the container opening. The device is simple in structure, convenient and quick, the mixed liquid production process is almost completely isolated from the outside environment, the pollution probability is reduced, and the biological safety is high.
Description
Technical field
The present invention relates to technical field of medical instruments, particularly a kind of blood sample sampling pretreatment device and method thereof.
Background technology
The collection of specimens of existing external diagnosis reagent micro blood, many employings transfer pipet and centrifuge tube carry out the mixing of blood sample and sample treatment solution, but this hybrid mode can cause blood sample and sample treatment solution directly to contact with external environment condition, and duration of contact is long, thus causing sample to pollute, biological safety is lower.
Summary of the invention
The object of the invention is to overcome above-mentioned deficiency, a kind of blood sample sampling pretreatment device and method thereof are provided, by siphon principle, avoid blood sample and sample treatment solution directly contacts with external environment condition, during mixing and external environment completely cut off, effectively prevent sample contaminated.
For achieving the above object, technical solution of the present invention there is provided a kind of blood sample sampling pretreatment device, comprising: the blood sampling portion removably connected and sample mixing unit; Described blood sampling portion comprises the suction pipe of a hollow, one end of described suction pipe is communicated with elastic body, described elastomeric internal package has can the compression stroke of elastomeric elastic wall described in resilience, described elastic wall is provided with compression opening, the junction of described compression opening and described suction pipe is provided with an O-ring seal, and the both sides of described O-ring seal are provided with the tack coat of the junction of the described compression opening of sealing and described suction pipe; Described sample mixing unit comprises the mixer for collecting suction pipe, Lower Half in described mixer is provided with the diaphragm seal supplying the end of described suction pipe to puncture, the wall of described diaphragm seal and described mixer is formed with the accommodating layer of airtight treating fluid, the bottom of described mixer is also provided with the discharge opeing unit be communicated with the accommodating layer of described treating fluid, the top of described mixer is provided with the vessel port of size corresponding to the caliber of described suction pipe, is fixed in described vessel port for described elastic body.
Preferably, baffle plate is provided with in the described mixer of vessel port, described baffle plate is provided with the connecting hole of corresponding described suction pipe caliber, the wall of described baffle plate and described mixer is formed with installing space, be provided with guiding piece in described installing space, in the middle part of described guiding piece, be provided with the guiding groove of corresponding described suction pipe external diameter.
Preferably, described baffle plate connecting hole perisporium is provided with the elastic packaging flexible glue body arranged towards mixer bottom direction.
Preferably, described elastic packaging flexible glue body has the ring-type flexible glue body of hollow, and is connected and the centrum connecting portion of volume convergent with described flexible glue body.
Preferably, described centrum connecting portion comprises multiple arc flap valves of conflicting and arranging.
Preferably, the junction of described elastic body and described suction pipe is formed with the draw-in groove that xsect is L-type, and described vessel port is conflicted with described draw-in groove and is connected.
Preferably, described sample mixing unit also comprises the over cap of airtight container opening, and described over cap has the described elastomeric guard space of collecting.
Preferably; described over cap has elastomeric first lid of collecting; and be connected and the second lid extended towards described mixer bottom direction with described first lid; described second lid ring set is on the sidewall of mixer; and described second lid is provided with groove ring, described mixer respective slot ring position is provided with bulge loop.
Preferably, described first lid has the first lid mouth of corresponding vessel port, described first lid mouth is provided with the easy abscission ring drawn in towards middle part, described easy abscission ring size corresponding to the tube wall of described suction pipe is arranged, described easy abscission ring is provided with rubber finger sleeve, and the opening of described rubber finger sleeve fits on described easy abscission ring.
A kind of blood sample sampling preprocess method, comprises the steps: 1) blood sampling, suction pipe is placed in blood sample and carries out blood sampling; 2) after blood sampling, carry out sample mixing, inserting mixer by gathering the suction pipe having blood sample, puncturing diaphragm seal by suction pipe, blood sample being injected the accommodating layer for the treatment of fluid and mix with treating fluid.
Blood sample sampling pretreatment device provided by the present invention, by the suction pipe automatic sucking blood sample of hollow, encapsulation process liquid in sample mixing unit, decrease both as much as possible to contact with external environment condition, blood sampling portion and sample mixing unit removably connect and can realize the rapid abutting joint of blood sampling and treating fluid, during mixing, only suction pipe need be punctured diaphragm seal, extrude elastic body again and inject blood sample to mixed liquor, stand-by after rocking mixer, need only discharge opeing unit to be opened when using, again extrude elastic body and can discharge mixed liquor.This apparatus structure is simple, almost completely isolated with external environment condition in fast and easy, mixed liquor manufacturing process, reduce contaminated probability and biological safety is high.
Blood sample sampling preprocess method provided by the invention, be placed in blood sample by suction pipe and carry out blood sampling, mixer is inserted by gathering the suction pipe having blood sample, diaphragm seal is punctured by suction pipe, blood sample is injected the accommodating layer for the treatment of fluid to mix with treating fluid, the isolation of whole process and external environment condition, the mixed liquor produced can not be polluted by external environment condition and the security of biology is high.
Accompanying drawing explanation
Fig. 1 is the structural representation of blood sample sampling pretreatment device of the present invention;
Fig. 2 is the structural representation of sample mixing unit in blood sample sampling pretreatment device of the present invention;
Fig. 3 is the enlarged drawing compressing opening and suction pipe junction in blood sample sampling pretreatment device of the present invention;
Fig. 4 is the flow process frame diagram of blood sample of the present invention sampling preprocess method.
Embodiment
For the benefit of to the understanding of structure of the present invention, be described below in conjunction with drawings and Examples.
Fig. 1 is the structural representation of blood sample sampling pretreatment device of the present invention, Fig. 2 is the structural representation of sample mixing unit in blood sample sampling pretreatment device of the present invention, and Fig. 3 is the enlarged drawing compressing opening and suction pipe junction in blood sample sampling pretreatment device of the present invention.Shown in composition graphs 1 to Fig. 3, the invention provides a kind of blood sample sampling pretreatment device, comprising: the blood sampling portion 10 removably connected and sample mixing unit 20.
Described blood sampling portion 10 comprises the suction pipe 11 of a hollow, one end of described suction pipe 11 is communicated with elastic body 12, the internal package of described elastic body 12 has can the compression stroke of elastomeric elastic wall described in resilience, described elastic wall is provided with compression opening 121, described compression opening 121 is provided with an O-ring seal 41 with the junction of described suction pipe 11, and the both sides of described O-ring seal 41 are provided with the tack coat 42 of the junction of the described compression opening 121 of sealing and described suction pipe, this setting avoids suction pipe 11 and directly bonds with elastic body 12, three layers of airtight protection are achieved by the form of tack coat 42-O-ring seal 41-tack coat 42, by the inner space in blood sampling portion 10 and external environment condition completely isolated.In the present embodiment, it is pointed that the front end of described suction pipe 11 is set to thorn.
Described sample mixing unit 20 comprises the mixer 21 for collecting suction pipe 11, Lower Half in described mixer 21 is provided with the diaphragm seal 211 supplying the end of described suction pipe 11 to puncture, described diaphragm seal 211 is formed with the accommodating layer 212 of airtight treating fluid with the wall of described mixer 21, the bottom of described mixer 21 is also provided with the discharge opeing unit 213 that layer 212 accommodating with described treating fluid is communicated with, the top of described mixer 21 is provided with the vessel port 214 with the corresponding size of caliber of described suction pipe 11, is fixed in described vessel port 214 for described elastic body 12.
Baffle plate 215 is provided with in the described mixer 21 of vessel port 213, described baffle plate 215 is provided with the connecting hole 2151 of corresponding described suction pipe 11 caliber, described baffle plate 215 is formed with installing space 216 with the wall of described mixer 21, be provided with guiding piece 218 in described installing space 216, in the middle part of described guiding piece 218, be provided with the guiding groove 2181 of corresponding described suction pipe external diameter.While wearing mixer 21 at suction pipe 11, guided by guiding groove 2181 pairs of suction pipes.
Connecting hole 2151 perisporium of described baffle plate 215 is provided with the elastic packaging flexible glue body 217 arranged towards mixer 21 bottom direction, in the present embodiment, described elastic packaging flexible glue body 217 has the ring-type flexible glue body 2171 of hollow, and is connected and the centrum connecting portion 2172 of volume convergent with described flexible glue body.Further, described centrum connecting portion 2172 comprises multiple arc flap valves of conflicting and arranging, when not plugging suction pipe, multiple arc flap valve is closure state, enter in mixer 21 to prevent from external contaminants matter, after plugging suction pipe 11, multiple arc flap valve is wrapped on the tube wall of suction pipe 11, also can prevent external contamination material from entering in mixer.
Further, described elastic body 12 is formed with the junction of described suction pipe 11 draw-in groove that xsect is L-type, and described vessel port 214 is conflicted with described draw-in groove and is connected.This is arranged so that elastic body 12 can be seated above vessel port 214, and after the draw-in groove conflict vessel port 214 of L-type, further, has completely cut off inner space and contacted with external environment condition.
In addition, described sample mixing unit 20 also comprises the over cap 30 of airtight container opening, and described over cap 30 has the guard space of accommodating described elastic body 12.In the present embodiment; described over cap 30 has elastomeric first lid 31 of collecting; and be connected and the second lid 32 extended towards described mixer 21 bottom direction with described first lid 31; described second lid 32 ring set is on the sidewall of mixer 21; and described second lid 32 is provided with groove ring 321, described mixer 21 respective slot ring 321 position is provided with bulge loop 22.Further, described first lid 31 has the first lid mouth of corresponding vessel port 214, described first lid mouth is provided with the easy abscission ring drawn in towards middle part, described easy abscission ring is arranged with the corresponding size of tube wall of the elastic body 12 of cylindrical shape, described easy abscission ring is provided with rubber finger sleeve, and the opening of described rubber finger sleeve fits on described easy abscission ring.When covering over cap 30 in vessel port 214, elastic body 12 through the hollow open of easy abscission ring, directly cause easy abscission ring conflict by airtight container opening 214, break easy abscission ring fast, final latex cover is wrapped in vessel port.This setting is further advanced by the link of rubber finger sleeve to elastic body 12 and vessel port 214 and seals, and further reduces contaminated possibility.Need cause concern, discharge opeing unit 213 has the discharging tube be located at bottom mixer 21, and described discharging tube is provided with the discharge opeing plug removably connected.
Fig. 4 is the flow process frame diagram of blood sample of the present invention sampling preprocess method, refers to Fig. 4, present invention also offers a kind of blood sample sampling preprocess method, comprises the steps:
Step S1, blood sampling, is placed in blood sample and carries out blood sampling by suction pipe.
Step S2, after blood sampling, carries out sample mixing, inserting mixer, puncturing gathering the suction pipe having blood sample diaphragm seal, blood sample is injected the accommodating layer for the treatment of fluid and mix with treating fluid by suction pipe.
Below the embody rule details of blood sample sampling pretreatment device provided by the invention is described in detail.
First, the suction pipe 11 in blood sampling portion 10 is placed in blood sample, pass through siphon principle, blood sample sucks in suction pipe 11 automatically, the suction pipe 11 sucking blood sample is inserted mixer 21 by vessel port, when suction pipe 11 enters guiding groove 2181, the inwall of guiding piece 218 contacts with suction pipe 11, plant fast to guide suction pipe 11, suction pipe 11 is continued downward, until the front end of suction pipe 11 punctures diaphragm seal 211, makes suction pipe 11 be inserted in the accommodating layer 212 for the treatment of fluid, now, elastic body 12 is just in time seated on airtight container 214 opening.When temporarily not needing mixing blood sample and treating fluid; directly over cap 30 is located in vessel port 214; in this process; elastic body 12 is through the hollow open of easy abscission ring; easy abscission ring break by airtight container 214 opening, thus rubber finger sleeve is wrapped in vessel port 214, now; described second lid 32 ring set is on the sidewall of mixer, and described second lid 32 is provided with, and groove ring is corresponding with the bulge loop of mixer to be engaged.When needing mixing blood sample and treating fluid; take out over cap, then extrude elastic body 12, so that blood sample is injected treating fluid; manually rocking mixer 21 makes mixed liquor mix, and the discharge opeing unit 213 finally by mixer 21 discharges mixed liquor.
Blood sample sampling pretreatment device provided by the present invention, by the suction pipe automatic sucking blood sample of hollow, encapsulation process liquid in sample mixing unit, decrease both as much as possible to contact with external environment condition, blood sampling portion and sample mixing unit removably connect and can realize the rapid abutting joint of blood sampling and treating fluid, during mixing, only suction pipe need be punctured diaphragm seal, extrude elastic body again and inject blood sample to mixed liquor, stand-by after rocking mixer, need only discharge opeing unit to be opened when using, again extrude elastic body and can discharge mixed liquor.Finish using closed discharge opeing plug, residual samples and device, together as offal treatment, effectively reduce sample to the impact of environment.This apparatus structure is simple, almost completely isolated with external environment condition in fast and easy, mixed liquor manufacturing process, reduce contaminated probability and biological safety is high.
Blood sample sampling preprocess method provided by the invention, be placed in blood sample by suction pipe and carry out blood sampling, mixer is inserted by gathering the suction pipe having blood sample, diaphragm seal is punctured by suction pipe, blood sample is injected the accommodating layer for the treatment of fluid to mix with treating fluid, the isolation of whole process and external environment condition, the mixed liquor produced can not be polluted by external environment condition and the security of biology is high.
Below embodiment is to invention has been detailed description by reference to the accompanying drawings, and those skilled in the art can make many variations example to the present invention according to the above description.Thus, some details in embodiment should not form limitation of the invention, the present invention by the scope that defines using appended claims as protection domain.
Claims (10)
1. a blood sample sampling pretreatment device, is characterized in that, comprising: the blood sampling portion removably connected and sample mixing unit;
Described blood sampling portion comprises the suction pipe of a hollow, one end of described suction pipe is communicated with elastic body, described elastomeric internal package has can the compression stroke of elastomeric elastic wall described in resilience, described elastic wall is provided with compression opening, the junction of described compression opening and described suction pipe is provided with an O-ring seal, and the both sides of described O-ring seal are provided with the tack coat of the junction of the described compression opening of sealing and described suction pipe;
Described sample mixing unit comprises the mixer for collecting suction pipe, Lower Half in described mixer is provided with the diaphragm seal supplying the end of described suction pipe to puncture, the wall of described diaphragm seal and described mixer is formed with the accommodating layer of airtight treating fluid, the bottom of described mixer is also provided with the discharge opeing unit be communicated with the accommodating layer of described treating fluid, the top of described mixer is provided with the vessel port of size corresponding to the caliber of described suction pipe, is fixed in described vessel port for described elastic body.
2. blood sample sampling pretreatment device according to claim 1, it is characterized in that, baffle plate is provided with in the described mixer of vessel port, described baffle plate is provided with the connecting hole of corresponding described suction pipe caliber, the wall of described baffle plate and described mixer is formed with installing space, be provided with guiding piece in described installing space, in the middle part of described guiding piece, be provided with the guiding groove of corresponding described suction pipe external diameter.
3. blood sample sampling pretreatment device according to claim 2, is characterized in that, described baffle plate connecting hole perisporium is provided with the elastic packaging flexible glue body arranged towards mixer bottom direction.
4. blood sample sampling pretreatment device according to claim 3, is characterized in that, described elastic packaging flexible glue body has the ring-type flexible glue body of hollow, and is connected and the centrum connecting portion of volume convergent with described flexible glue body.
5. blood sample sampling pretreatment device according to claim 4, is characterized in that, described centrum connecting portion comprises multiple arc flap valves of conflicting and arranging.
6. blood sample sampling pretreatment device according to claim 5, is characterized in that, the junction of described elastic body and described suction pipe is formed with the draw-in groove that xsect is L-type, and described vessel port is conflicted with described draw-in groove and is connected.
7. blood sample sampling pretreatment device according to claim 6, is characterized in that, described sample mixing unit also comprises the over cap of airtight container opening, and described over cap has the described elastomeric guard space of collecting.
8. blood sample sampling pretreatment device according to claim 7; it is characterized in that; described over cap has elastomeric first lid of collecting; and be connected and the second lid extended towards described mixer bottom direction with described first lid; described second lid ring set is on the sidewall of mixer; and described second lid is provided with groove ring, described mixer respective slot ring position is provided with bulge loop.
9. blood sample sampling pretreatment device according to claim 8, it is characterized in that, described first lid has the first lid mouth of corresponding vessel port, described first lid mouth is provided with the easy abscission ring drawn in towards middle part, described easy abscission ring size corresponding to the tube wall of described suction pipe is arranged, described easy abscission ring is provided with rubber finger sleeve, and the opening of described rubber finger sleeve fits on described easy abscission ring.
10. according to claim 1 to 9 any one blood sample sampling pretreatment device blood sample sampling preprocess method, it is characterized in that, comprise the steps:
1) blood sampling, is placed in blood sample and carries out blood sampling by suction pipe;
2) after blood sampling, carry out sample mixing, inserting mixer by gathering the suction pipe having blood sample, puncturing diaphragm seal by suction pipe, blood sample being injected the accommodating layer for the treatment of fluid and mix with treating fluid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510867804.6A CN105510109B (en) | 2015-11-30 | 2015-11-30 | Blood sample sampling pretreatment device and its method |
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| CN201510867804.6A CN105510109B (en) | 2015-11-30 | 2015-11-30 | Blood sample sampling pretreatment device and its method |
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| CN105510109A true CN105510109A (en) | 2016-04-20 |
| CN105510109B CN105510109B (en) | 2018-08-10 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107569240A (en) * | 2017-08-30 | 2018-01-12 | 台州市黄岩方野科技发展有限公司 | A kind of micro blood-taking tube of preset reagent and blood sampling and the method for adding reagent |
| CN107782602A (en) * | 2016-08-31 | 2018-03-09 | 东芝医疗系统株式会社 | Sample preparation apparatus, check external member and sample modulator approach |
| CN110292961A (en) * | 2019-07-02 | 2019-10-01 | 英诺维尔智能科技（苏州）有限公司 | A kind of liquid transfer gun head sealing structure |
| CN111956272A (en) * | 2020-09-03 | 2020-11-20 | 河南省中医院（河南中医药大学第二附属医院） | Cervical cancer epithelial cell sampling device |
| CN118443414A (en) * | 2024-05-17 | 2024-08-06 | 盐城市第一人民医院(南通医学院第四附属医院) | Pretreatment device for test specimen and application method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107782602A (en) * | 2016-08-31 | 2018-03-09 | 东芝医疗系统株式会社 | Sample preparation apparatus, check external member and sample modulator approach |
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| CN111956272A (en) * | 2020-09-03 | 2020-11-20 | 河南省中医院（河南中医药大学第二附属医院） | Cervical cancer epithelial cell sampling device |
| CN118443414A (en) * | 2024-05-17 | 2024-08-06 | 盐城市第一人民医院(南通医学院第四附属医院) | Pretreatment device for test specimen and application method thereof |
| CN118443414B (en) * | 2024-05-17 | 2024-10-11 | 盐城市第一人民医院(南通医学院第四附属医院) | Pretreatment device for test specimen and application method thereof |
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| CN105510109B (en) | 2018-08-10 |
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Address after: 430074 No.18, East bio Garden Road, Donghu New Technology Development Zone, Wuhan City, Hubei Province Patentee after: Wuhan Jinghong Technology Co., Ltd Address before: 430074, building 7-5, 6 Industrial Park, Kanto New Technology Development Zone, Wuhan, Hubei, East Lake Patentee before: Wuhan Jinghong Wanfangtang Medical Technology Co., Ltd. |
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Denomination of invention: Blood sample sampling pretreatment device and method Effective date of registration: 20191220 Granted publication date: 20180810 Pledgee: Bank of Hankou Limited by Share Ltd Financial Services Center Pledgor: Wuhan Jinghong Technology Co., Ltd Registration number: Y2019980001183 |