CN212674526U - Aseptic sampler - Google Patents
Aseptic sampler Download PDFInfo
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- CN212674526U CN212674526U CN202021364666.2U CN202021364666U CN212674526U CN 212674526 U CN212674526 U CN 212674526U CN 202021364666 U CN202021364666 U CN 202021364666U CN 212674526 U CN212674526 U CN 212674526U
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Abstract
The utility model discloses an aseptic sampler, include: the sampling device comprises a sampling bottle, a bottle cap, a connecting pipe, a filter and a blocking device, wherein the bottle cap is connected with the sampling bottle, the connecting pipe penetrates through the bottle cap to be communicated with the sampling bottle, the blocking device is arranged on the part, located on the outer side of the bottle cap, of the connecting pipe, the filter comprises an air connector and a filter membrane, the air connector penetrates through the bottle cap to be communicated with the sampling bottle, and the filter membrane is arranged inside the air connector. The sterile sampler can improve the sampling efficiency of the cell culture solution and reduce the sampling cost of the cell culture solution.
Description
Technical Field
The utility model relates to a sample censorship technical field especially relates to an aseptic sampler.
Background
In the process of cell culture under the totally enclosed condition, the cell culture solution needs to be sampled and inspected regularly. However, the sampling process should not only ensure that the sterility requirements of the cell culture fluid are met, and no external pathogens are allowed to enter the cell culture environment, but also consider the viruses possibly existing in the cell culture fluid, and no influence of the viruses on operators and the external environment is allowed. Therefore, the sampling operation of the cell culture solution needs to be completed under a totally enclosed condition, the sampling operation is complex, the sampling efficiency is low and the sampling cost is high.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an aseptic sampler can improve the sample efficiency of cell culture liquid, reduces the sample cost of cell culture liquid.
To achieve the purpose, the utility model adopts the following technical proposal:
an aseptic sampler comprising: sampling bottle, bottle lid, connecting pipe, filter and blocking device, the bottle lid with the sampling bottle is connected, the connecting pipe passes the bottle lid with the sampling bottle intercommunication, the connecting pipe is located be provided with on the part in the bottle lid outside blocking device, the filter includes air connector and filter membrane, air connector passes the bottle lid with the sampling bottle intercommunication, the filter membrane sets up inside the air connector.
In one embodiment, the sterile sampler further comprises a negative pressure device connected to the air connector.
In one embodiment, the negative pressure device is a syringe.
In one embodiment, the sterile sampler further comprises a rubber plug and a rubber plug cover, wherein a sampling opening is formed in the sampling bottle, the rubber plug is partially inserted into the sampling opening, and the rubber plug cover is arranged at the sampling opening and connected with the sampling bottle.
In one embodiment, the sampling bottle is provided with an extension part, the extension part surrounds the sampling opening, and the extension part is provided with a sampling opening.
In one embodiment, the filter membrane is a hydrophobic filter membrane.
In one embodiment, the number of the filter membranes is two.
In one embodiment, the connecting tube is made of thermoplastic material.
In one embodiment, the blocking device is a snap plate or a caliper.
In one embodiment, an adhesive layer is arranged between the bottle cap and the sampling bottle.
When the aseptic sampler is used, the aseptic sampler is assembled and sterilized before cell culture, and the connecting pipe is connected to the cell culture circulating pipeline. When the cell culture solution needs to be sampled and inspected, the air connector of the filter is connected with a negative pressure source; then, opening the blocking device on the connecting pipe, and starting the negative pressure source to pump air so that the cell culture solution in the cell culture circulating pipeline flows into the sampling bottle through the connecting pipe; and (4) closing the blocking device after obtaining a proper amount of cell culture solution, fusing the connecting pipe, taking away the sterile sampler, and completing sampling operation.
The sterile sampler is provided with a blocking device on the connecting pipe, and a filter membrane is arranged in the air joint. Blocking device can form sealedly to remaining connecting pipe after the connecting pipe melts, avoids the outside air to get into the cell culture circulating line and causes the pollution to the cell culture fluid, and can avoid the cell culture fluid to reveal and produce the influence to operator and external environment. Set up the filter membrane in the air connection and can guarantee that sample bottle is inside to be kept apart with the aseptic of external environment, guarantee that the in-process of taking a sample can not cause the pollution to the cell culture liquid in the culture tube way and the cell culture liquid that the sample obtained.
In conclusion, the sterile sampler is assembled with the cell culture circulating pipeline before cell culture, the negative pressure source is connected during sampling, so that the cell culture solution can be sampled, the cell culture solution in the culture pipeline and the cell culture solution obtained by sampling can not be polluted by the external environment in the sampling process, the pollution of the cell culture solution to the external environment can be avoided, the cell culture solution sampling operation is simple and convenient, the sampling operation is safe and reliable, the sampling efficiency of the cell culture solution can be improved, and the sampling cost of the cell culture solution is reduced.
Drawings
FIG. 1 is a schematic diagram of the construction of a sterile sampler in one embodiment;
fig. 2 is a cross-sectional view of a portion of the structure of a sterile sampler in one embodiment.
Description of reference numerals:
10-sampling bottle, 11-extension part, 20-bottle cap, 30-connecting pipe, 41-air connector, 42-filter membrane, 50-blocking device, 61-rubber plug and 62-rubber plug cover.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Referring to fig. 1 to 2, an embodiment of a sterile sampler includes a sampling bottle 10, a bottle cap 20, a connecting pipe 30, a filter and a blocking device 50, wherein the bottle cap 20 is connected to the sampling bottle 10, the connecting pipe 30 passes through the bottle cap 20 and is communicated with the sampling bottle 10, the blocking device 50 is disposed on a portion of the connecting pipe 30 located outside the bottle cap 20, the filter includes an air connector 41 and a filter membrane 42, the air connector 41 passes through the bottle cap 20 and is communicated with the sampling bottle 10, and the filter membrane 42 is disposed inside the air connector 41.
Specifically, the bottle cap 20 is provided with a first through hole and a second through hole, the connecting pipe 30 is communicated with the sampling bottle 10 through the first through hole, and the air connector 41 is communicated with the sampling bottle 10 through the second through hole.
When the aseptic sampler is used, the aseptic sampler is assembled and sterilized before cell culture, and the connection pipe 30 is connected to the cell culture circulation pipeline. When the cell culture solution needs to be sampled and tested, the air connector 41 of the filter is connected with a negative pressure source; then, the blocking device 50 on the connecting pipe 30 is opened, and the negative pressure source is started to pump air so that the cell culture solution in the cell culture circulating pipeline flows into the sampling bottle through the connecting pipe; after a suitable amount of cell culture fluid is obtained, the blocking device 50 is closed, the connecting tube 30 is fused, and the sterile sampler is taken away to complete the sampling operation.
The sterile sampler described above is provided with the blocking device 50 in the connection tube 30, and the filter 42 in the air connection 41. Specifically, blocking device 50 can be but not limited to cardboard or calliper, and blocking device 50 can form sealedly to remaining connecting pipe 30 after connecting pipe 30 melts, avoids the outside air to get into the cell culture circulation pipeline and causes the pollution to the cell culture liquid, and can avoid the cell culture liquid to reveal and produce the influence to operator and external environment. The filter membrane 42 arranged in the air joint 40 can ensure the sterile isolation of the interior of the sampling bottle 10 from the external environment, and ensure that the cell culture solution in the culture pipeline and the cell culture solution obtained by sampling cannot be polluted in the sampling process.
In one embodiment, the sterile sampler further comprises a negative pressure device, which is connected to the air connection 41. The negative pressure device is used for providing negative pressure when sampling, and particularly, the negative pressure device can be but is not limited to a syringe. Further, in one embodiment, in order to improve the air tightness of the connection, the negative pressure device is connected with the connecting pipe through a luer connector.
In one embodiment, the sterile sampler further comprises a rubber plug 61 and a rubber plug cover 62, a sampling opening is formed on the sampling bottle 10, the rubber plug 61 is partially inserted into the sampling opening, and the rubber plug cover 62 is connected with the sampling bottle 61 at the sampling opening. Specifically, plug 61 and plug lid 62 are used for sealed sample connection, ensure that the cell culture liquid that the sample obtained is not contaminated, and plug 61 can be used for the syringe sample, use the syringe to pass the plug and can take out the cell culture liquid in the sampling bottle, and sample operation is simple and convenient.
In one embodiment, the sampling bottle 10 is provided with an extension portion 11, the extension portion 11 surrounds the sampling opening, and the extension portion 11 is provided with a sampling opening. Set up extension 11 and can protect plug 61 and plug lid 62, avoid the in-process plug 61 and plug lid 62 that remove aseptic sampler to collide with other article and take place to drop, ensure plug 61 and plug lid 62 sealed reliable and stable, set up the sample opening on extension 11 and can conveniently take off plug lid 62.
In one embodiment, the filter membrane 42 is a hydrophobic filter membrane, specifically, the filter membrane 42 has a thickness of 0.1 μm. The filter membrane 42 can block the cell culture fluid in the sampling bottle 10, prevent the cell culture fluid from overflowing through the air connector 41, and can filter the air, so that the inside of the sampling bottle 10 is ensured to be aseptically isolated from the external environment, and the cell culture fluid in the culture pipeline and the cell culture fluid obtained by sampling are ensured not to be polluted in the sampling process. Further, in one embodiment, to further improve the sealing and sterility safety of the sampling bottle 10, the number of the filter membranes 42 is two, and the two filter membranes 42 are spaced apart from each other in the air connection 41.
In one embodiment, the connection tube 30 is made of thermoplastic material, the connection tube 30 can be thermally melted by the welding operation of the welding machine, and forms a self-sealing at the welding position, and the fusing of the connection tube 30 forms the self-sealing to ensure the sealing property of the connection tube 30, so as to ensure that neither the cell culture fluid in the culture pipeline nor the cell culture fluid sampled in the sampling bottle 10 after the fusing of the connection tube 30 is polluted.
In one embodiment, an adhesive layer is disposed between the bottle cap 20 and the sampling bottle 10, and the bottle cap 20 and the sampling bottle 10 are bonded together to ensure the sealing performance of the sterile sampler. In one embodiment, to further improve the sealing performance of the sterile sampler, an adhesive layer is disposed between the bottle cap 20 and the connecting tube 30 and the air connector 40. And an adhesive layer is also arranged between the rubber plug 61 and the sampling bottle 10.
Specifically, the specific use of the sterile sampler described above is as follows: firstly, before cell culture, assembling and sterilizing a sterile sampler, and connecting a connecting pipe 30 to a cell culture circulating pipeline; then, when the cell culture solution needs to be sampled and tested, the air connector 41 of the filter is connected with the injector through a luer connector; then, the blocking device 50 on the connection tube 30 is opened, and the syringe is pulled so that a proper amount of cell culture fluid flows into the sampling bottle 10; after a proper amount of cell culture solution is obtained, pushing the injector to enable the liquid in the connecting pipe 30 to flow back to the cell culture circulating pipeline, then closing the blocking device 50 on the connecting pipe 30, and carrying out welding operation (specifically, multi-point welding can be carried out) on the connecting pipe 30 below the blocking device 50 by using a handheld welding machine to enable the connecting pipe 30 to be hot-melted and form self-sealing at the welding position; finally, the connection tube 30 is cut off at the fusing position, the sampler is taken away, and the sampling operation is completed.
The sterile sampler is assembled with the cell culture circulating pipeline in advance before cell culture, the negative pressure device is connected during sampling, the cell culture solution can be sampled, the sampling operation of the cell culture solution is simple and convenient, the sampling operation is safe and reliable, the sampling efficiency of the cell culture solution can be improved, and the sampling cost of the cell culture solution is reduced.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (10)
1. An aseptic sampler, comprising: sampling bottle (10), bottle lid (20), connecting pipe (30), filter and blocking device (50), bottle lid (20) with sampling bottle (10) are connected, connecting pipe (30) are passed bottle lid (20) with sampling bottle (10) intercommunication, connecting pipe (30) are located be provided with on the part in bottle lid (20) outside blocking device (50), the filter includes air joint (41) and filter membrane (42), air joint (41) pass bottle lid (20) with sampling bottle (10) intercommunication, filter membrane (42) set up inside air joint (41).
2. Sterile sampler according to claim 1, characterized in that it further comprises a negative pressure device connected to the air connection (41).
3. An aseptic sampler as claimed in claim 2, in which the negative pressure device is a syringe.
4. The sterile sampler of claim 1, further comprising a rubber plug (61) and a rubber plug cover (62), wherein the sampling bottle (10) has a sampling opening, the rubber plug (61) is partially inserted into the sampling opening, and the rubber plug cover (62) is connected to the sampling bottle (10) at the sampling opening.
5. The sterile sampler of claim 4, wherein the sampling bottle (10) is provided with an extension part (11), the extension part (11) is enclosed outside the sampling opening, and the extension part (11) is provided with a sampling opening.
6. Sterile sampler according to any of claims 1 to 5, characterized in that the filter membrane (42) is a hydrophobic filter membrane.
7. Sterile sampler according to claim 6, characterized in that the number of filter membranes (42) is two.
8. Sterile sampler according to any of claims 1 to 5, characterized in that the connecting tube (30) is made of a thermoplastic material.
9. Sterile sampler according to any of claims 1 to 5, characterized in that the blocking means (50) is a snap-plate or a caliper.
10. Sterile sampler according to any of claims 1 to 5 characterized in that an adhesive layer is provided between the bottle cap (20) and the sampling bottle (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021364666.2U CN212674526U (en) | 2020-07-13 | 2020-07-13 | Aseptic sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021364666.2U CN212674526U (en) | 2020-07-13 | 2020-07-13 | Aseptic sampler |
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| Publication Number | Publication Date |
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| CN212674526U true CN212674526U (en) | 2021-03-09 |
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| CN202021364666.2U Active CN212674526U (en) | 2020-07-13 | 2020-07-13 | Aseptic sampler |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
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2020
- 2020-07-13 CN CN202021364666.2U patent/CN212674526U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
| WO2023284580A1 (en) * | 2021-07-13 | 2023-01-19 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
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