CN105499261A - Treatment method for improving heavy metal polluted soil remediation efficiency through photinia serrulata - Google Patents
Treatment method for improving heavy metal polluted soil remediation efficiency through photinia serrulata Download PDFInfo
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- 239000002689 soil Substances 0.000 title claims abstract description 51
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000005067 remediation Methods 0.000 title claims abstract description 9
- 241001243666 Photinia serratifolia Species 0.000 title abstract 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 12
- XEYBHCRIKKKOSS-UHFFFAOYSA-N disodium;azanylidyneoxidanium;iron(2+);pentacyanide Chemical compound [Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].[O+]#N XEYBHCRIKKKOSS-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229940083618 sodium nitroprusside Drugs 0.000 claims abstract description 8
- 238000005507 spraying Methods 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims description 34
- 241001163113 Photinia glabra Species 0.000 claims description 33
- BQMKAHQKDSZAIQ-UHFFFAOYSA-N tetrasodium;iron(3+);nitroxyl anion;pentacyanide Chemical compound [Na+].[Na+].[Na+].[Na+].[Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].O=[N-] BQMKAHQKDSZAIQ-UHFFFAOYSA-N 0.000 claims description 29
- 239000007921 spray Substances 0.000 claims description 19
- 238000003672 processing method Methods 0.000 claims description 14
- 239000010949 copper Substances 0.000 claims description 10
- 229910052793 cadmium Inorganic materials 0.000 claims description 8
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims description 8
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 6
- 229910052802 copper Inorganic materials 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 4
- 230000004083 survival effect Effects 0.000 claims description 4
- 238000002054 transplantation Methods 0.000 claims description 3
- 241000607479 Yersinia pestis Species 0.000 claims description 2
- 201000010099 disease Diseases 0.000 claims description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 2
- 239000012452 mother liquor Substances 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 7
- 229960001484 edetic acid Drugs 0.000 abstract 2
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 30
- 238000009825 accumulation Methods 0.000 description 13
- 238000005516 engineering process Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 239000002574 poison Substances 0.000 description 4
- 231100000614 poison Toxicity 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 206010027439 Metal poisoning Diseases 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 208000010501 heavy metal poisoning Diseases 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000006353 environmental stress Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000705 flame atomic absorption spectrometry Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000013138 pruning Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 231100000693 bioaccumulation Toxicity 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000010220 ion permeability Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052752 metalloid Inorganic materials 0.000 description 1
- 150000002738 metalloids Chemical class 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000003802 soil pollutant Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- JBQYATWDVHIOAR-UHFFFAOYSA-N tellanylidenegermanium Chemical compound [Te]=[Ge] JBQYATWDVHIOAR-UHFFFAOYSA-N 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Cultivation Of Plants (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a treatment method for improving heavy metal polluted soil remediation efficiency through photinia serrulata. The method comprises the following steps: a, transplanting photinia serrulata saplings to the heavy metal polluted soil, and spraying an EDTA (Ethylene Diamine Tetraacetic Acid) solution to the soil at the root parts of the photinia serrulata saplings after the photinia serrulata saplings live stably; b, spraying 100ml sodium nitroprusside with the concentration of 0.01 to 0.2mmol/L to the soil at the root part of each photinia serrulata sapling after 0 to 48 hours; c, spraying 20ml sodium nitroprusside with the concentration of 0.01 to 0.2mmol/L to the leaves of each photinia serrulata sapling after 5 days. The method has the main effect that the heavy metal polluted soil remediation efficiency can be obviously improved on the premise that the ornamental value of the photinia serrulata is not influenced.
Description
Technical field
The invention belongs to the recovery technique field of heavy metal environmental pollution, be specifically related to a kind ofly improve the processing method of photinia glabra to heavy metal pollution of soil remediation efficiency.
Background technology
Soil is that the mankind depend on for existence the necessary means of production of development, along with industrialized deeply and the aggravation of the unreasonable activity of the mankind, the multiple pollutant containing heavy metal enters soil by all means.Cause the enrichment of corresponding heavy metal in soil.Mainly with plumbous (Pb), cadmium (Cd), mercury (Hg), copper (Cu), zinc (Zn) and metalloid arsenic (As) etc. in soil.Heavy metal element have in soil stable, be easy to accumulation and the not easily characteristic such as removal, and jeopardize the health of the mankind by food chain.The great contamination accident such as " cadmium rice " event, " blood lead event " has caused unmeasurable loss.
At present, conventional heavy metal pollution of soil recovery technique mainly contains physical method, chemical method and biological method.And these physics or chemical method more or less also exist expensive, easy damage field ground structure, change the original physicochemical property of soil and bring secondary pollution problem.Biological method, namely phytoremediation technology.Compare other physics or chemical method, it is high and can not to advantages such as soil have a negative impact that phytoremediation technology has small investment, security.Become one of method of most potentiality and prospect in heavy metal pollution of soil recovery technique.
Photinia glabra, evergreen dungarunga, its fast growth, and by force germinating, resistance to pruning, the discarded branches and leaves after pruning can focus on.It is mainly used in garden landscape greening, makes scape etc., does not enter food chain, if be applied to phytoremediation technology field, then have only sky and obtain thick inherent advantage.But the plant that at present phytoremediation technology mainly relies on various heavy metal to have enrichment or super accumulation ability is main, photinia glabra etc. are to the poor landscape flower plant then rare research of heavy metal-polluted soil stress tolerance.
Its general principle of phytoremediation technology utilizes root system of plant to absorb the heavy metal contaminants in soil, and be transported to the object reaching in the cauline leaf of aerial part and remove heavy metal in soil pollutant.Therefore, the efficiency of phytoremediation depends on that plant is to the absorption of various heavy metal contaminants and transhipment accumulation capability substantially.In soil, add specific chelating agent artificially can activate the heavy metal ion discharging and fixed by solid phase of soil, make it dissolve and enter the soil liquid, thus improve the absorption bioaccumulation efficiency of plant heavy metal.But this will certainly damage plant, directly affects its landscape planting effect and sight to landscape flower plants such as photinia glabras.
Nitric oxide (NO) is a kind of key signal molecule be prevalent in organism, and biological degeneration-resistant border Stress responses and defense reaction etc. all regulate and control closely related with NO.In plant heavy metallic poison, exogenous NO gas plays a crucial role.There are some researches show that exogenous NO gas can alleviate the Lipid peroxidation metabolism degree of injury of wheat root under Cd stress, reduce ion permeability and H
2o
2content, and reduce the indexs such as activities of antioxidant enzymes such as SOD to alleviate heavy metallic poison symptom.In recent years in the research alleviating heavy metallic poison, exogenous NO gas has become the study hotspot in this field.But not yet find that there is and exogenous NO gas is applied to the research that photinia glabra alleviates heavy metal stress aspect.
Summary of the invention
The present invention, in order to solve the problem, provide a kind of and improves the processing method of photinia glabra to heavy metal pollution of soil remediation efficiency, reach the object realizing the repairing effect to heavy metal pollution of soil under the prerequisite not affecting photinia glabra view.
For achieving the above object, the present invention is by the following technical solutions:
The invention provides and a kind ofly improve the processing method of photinia glabra to heavy metal pollution of soil remediation efficiency, said method comprising the steps of:
Step a, photinia glabra transplantation of seedlings, in heavy metal pollution of soil soil, after the stable survival of plant to be planted, sprays EDTA solution in photinia glabra seedling root soil;
After step b, 0-48 hour, in plant root soil, spray concentration is the sodium nitroprussiate (SNP) of 0.01-0.2mmol/L, and consumption is 100ml/ strain;
Step c, sprayed again to blade after 5 days, the sodium nitroprussiate of 0.01-0.2mmol/L, and consumption is 20ml/ strain.
Heavy-metal contaminated soil described in the present invention at least meets one of following condition: cadmium content scope is at 0.2-100mg/kg, and lead content scope is at 20-3000mg/kg, and copper content range is at 20-3000mg/kg.
Further, in step a, photinia glabra seedling plant height is 20-50cm, and branches and leaves are luxuriant, without disease and pest.
Further, in step a, EDTA solution concentration is 10mmol/L.
Further, in step a, EDTA spray solution consumption is 150ml/ strain.
Further, sodium nitroprusside solution is that distilled water dissolves, and be mixed with the mother liquor of 100mmol/L, 4 DEG C of preservations, the used time is diluted to desired concn again.
Further, in step b, sodium nitroprusside solution spraying time is after 24h.
Further, in step b, sodium nitroprusside solution spray concentration is 0.1mmol/L.
Compared with prior art, the technical program has the following advantages:
In the present invention by photinia glabra transplantation of seedlings to heavy-metal contaminated soil, after the stable survival of plant to be planted, spray EDTA solution in soil after, heavy metal in soil ion activation, photinia glabra seedling absorbs heavy metal in a large number from soil, transports simultaneously and accumulate to aerial part.Heavy metal is constantly deposited in photinia glabra shoot root portion, the cell membrane of stem stalk and blade, cytoplasm, vacuole or blade cuticula, and photinia glabra seedling there will be heavy metal poisoning symptom very soon, blade yellowing, curling, withered.First time sprays after SNP, and the heavy metal poisoning symptom of photinia glabra seedling is obviously alleviated, and improves the tolerance that plant heavy metal coerces.After again spraying, plant leaf blade stretches, recovers original color and luster, does not substantially affect its sight.
The living environment of plant is not be always suitable for, and the process of growth and development of plants is often subject to various environment-stress (also claiming adverse circumstance).Plant from heavy metallic poison, exogenous NO gas plays a crucial role.Research finds that exogenous NO gas is coerced through plant root cells internal oxidition that is plumbous and cadmium induction in alleviation, alleviates the growth inhibition of root system, strengthens SOD activity etc. in cell and all plays crucial regulating and controlling effect; Also studies have reported that exogenous NO gas injures by the environment stress that alleviates of regulating plant vivo oxidation metaboilic level; NO is the important member in plant heavy metal defence signal transduction system.These results of study are pointed out: by applying exogenous NO gas or the intracellular NO level of regulating plant to plant, can the regulating plant heavy metal tolerance of coercing effectively, improve the enriching quantity of plant heavy metal, thus the remediation efficiency of plant to heavy metal pollution of soil can be improved.
The experiment proved that, spray EDTA solution in soil after, different time sections provides various dose NO sustained release agent SNP to photinia glabra seedling, there is provided compared with exogenous NO gas with not giving photinia glabra seedling, 0h, 12h, 24h, 48h spray SNP, the heavy metal accumulation amount of stem stalk and blade increases all to some extent, and has obvious mitigation to plant heavy metal poisoning symptom.When wherein 24h sprays, the heavy metal accumulation amount of stem stalk and blade is apparently higher than 0h, 12h, 48h.SNP spray concentration take 0.1mmol/L as the best.
Effect of the present invention is mainly reflected in: under the prerequisite not affecting flower plant sight, can significantly improve the efficiency that plant is repaired heavy metal pollution of soil.
Accompanying drawing explanation
Fig. 1 is that sodium nitroprussiate (SNP) spraying time is on the impact of Photinia glabra leaves heavy metal accumulation amount.
Fig. 2 is the impact of sodium nitroprussiate (SNP) spraying time on photinia glabra stem heavy metal accumulation amount.
Fig. 3 is that sodium nitroprussiate (SNP) spray concentration is on the impact of Photinia glabra leaves heavy metal accumulation amount.
Fig. 4 is the impact of sodium nitroprussiate (SNP) spray concentration on photinia glabra stem heavy metal accumulation amount.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
That need repair supplies examination soil collecting and Chun Jian township of Fuyang City of Zhejiang Province mining site, and sampling depth is 0-20cm, and it is for subsequent use that soil crosses 4mm sieve after room temperature is air-dry.After measured, as follows for examination soil physico-chemical property: pH (H
2o) value 5.82, content of organic matter 20.87g/kg, CEC value 10.13cmol/kg, plumbous (Pb) 85.64mg/kg, cadmium (Cd) 5.84mg/kg, copper (Cu) 79.78mg/kg, this experiment adopts greenhouse earth culture potted plant, is stirred by the examination soil that supplies after air-dry, packing, 3kg/ basin, transplanted by uniform for growing way photinia glabra (kind is red guest sieve) plant seedling to supplying in examination soil, appropriateness is watered, the stable survival of plant after 30 days, in soil, spray the EDTA solution of 20mmol/L, fountain height is 200ml/kg, and period carries out conventional maintenance of watering.
Test process one: sodium nitroprussiate (SNP) the suitableeest time of application.Be set to 4 different time points: (1) and EDTA are simultaneously (after 0 hour); After (2) 12 hours; After (3) 24 hours; After (4) 48 hours.
Processing method: each process is 10 strains (basins), and each process in triplicate.SNP concentration is 0.1mmol/L, is sprayed at soil and blade near root, sprays on a small quantity after 5 days to blade again according to 100ml/ strain consumption.Collecting part Photinia glabra leaves and stem stalk after 10 days, rinse three times successively with running water and distilled water, 90 DEG C of 30min that complete, and dries to constant weight for 55 DEG C, pulverizes and cross 40 orders (0.42mm) sieve, adopting HNO
3-HClO
4clear up, the content of the lead (Pb) in Flame Atomic Absorption Spectrometry spectroscopic mensuration digestion solution, cadmium (Cd), copper (Cu).The results are shown in Figure 1 and Fig. 2.
SNP is used by the known different times of test one, heavy metal accumulation amount difference all to some extent in Photinia glabra leaves and stem, with do not use compared with SNP, 0h, SNP is used after 12h, 24h, 48h, photinia glabra stem stalk and blade heavy metal accumulation amount increase all to some extent, but use at 24h, and in its stem stalk and blade, enriching quantity is apparently higher than other each periods.Therefore, use after 24h as optimum time.
Test process two: sodium nitroprussiate (SNP) the suitableeest amount of application.Be set to 4 variable concentrations: 0.01mmol/L, 0.05mmol/L, 0.1mmol/L, 0.2mmol/L and blank.
Processing method: the SNP solution of configuration 0.01 to 0.2mmol/L, is sprayed at soil and blade near root according to 150ml/ strain consumption, sprays on a small quantity after 5 days to blade again.Collecting part Photinia glabra leaves and stem stalk after 10 days, use running water and distilled water flushing three times, 90 DEG C of 30min that complete respectively, dries to constant weight for 55 DEG C, pulverizes and crosses 40 orders (0.42mm) and sieve, employing HNO
3-HClO
4clear up, the content of the lead (Pb) in Flame Atomic Absorption Spectrometry spectroscopic mensuration digestion solution, cadmium (Cd), copper (Cu).The results are shown in Figure 3 and Fig. 4.
From test two, SNP concentration from 0mmol/L, to 0.1mmol/L, photinia glabra stem stalk and blade heavy metal accumulation amount constantly increase, and reach maximum at 0.1mmol/L place.Afterwards, along with the raising of SNP concentration, its heavy metal accumulation amount no longer increases.Visible 0.1mmol/L is the optimal application concentration of SNP.
Although the present invention with preferred embodiment openly as above; but it is not for limiting the present invention; any those skilled in the art without departing from the spirit and scope of the present invention; the Method and Technology content of above-mentioned announcement can be utilized to make possible variation and amendment to technical solution of the present invention; therefore; every content not departing from technical solution of the present invention; the any simple modification done above embodiment according to technical spirit of the present invention, equivalent variations and modification, all belong to the protection domain of technical solution of the present invention.
Claims (8)
1. improve the processing method of photinia glabra to heavy metal pollution of soil remediation efficiency, it is characterized in that, said method comprising the steps of:
Step a, photinia glabra transplantation of seedlings, in heavy metal pollution of soil soil, after the stable survival of plant to be planted, sprays EDTA solution in photinia glabra seedling root soil;
After step b, 0-48 hour, in plant root soil, spray concentration is the sodium nitroprussiate of 0.01-0.2mmol/L, and consumption is 100ml/ strain;
Step c, sprayed again to blade after 5 days, the sodium nitroprussiate of 0.01-0.2mmol/L, and consumption is 20ml/ strain.
2. processing method according to claim 1, it is characterized in that, described heavy-metal contaminated soil is containing one or more in following three heavy metal species: cadmium content scope is at 0.2-100mg/kg, and lead content scope is at 20-3000mg/kg, and copper content range is at 20-3000mg/kg.
3. processing method according to claim 1, is characterized in that, in step a, photinia glabra seedling plant height is 20-50cm, and branches and leaves are luxuriant, without disease and pest.
4. processing method according to claim 1, is characterized in that, in step a, EDTA solution concentration is 10mmol/L.
5. processing method according to claim 1, is characterized in that, in step a, EDTA spray solution consumption is 150ml/ strain.
6. processing method according to claim 1, is characterized in that, sodium nitroprusside solution is that distilled water dissolves, and be mixed with the mother liquor of 100mmol/L, 4 DEG C of preservations, the used time is diluted to desired concn again.
7. processing method according to claim 1, is characterized in that, in step b, sodium nitroprusside solution spraying time is after 24h.
8. processing method according to claim 1, is characterized in that, in step b, sodium nitroprusside solution spray concentration is 0.1mmol/L.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101704016A (en) * | 2009-11-17 | 2010-05-12 | 上海市农业科学院 | Phytochemically combined remediation technology for soil contaminated by heavy metals, Mn, Pb, Cr and Zn |
| CN103039150A (en) * | 2013-01-14 | 2013-04-17 | 中国林业科学研究院亚热带林业研究所 | Efficient low-price rapid abandoned tailing vegetation restoration method |
| CN103962367A (en) * | 2014-04-15 | 2014-08-06 | 西南科技大学 | Method for promoting enrichment of cadmium by red spinach through composition of plant hormone and chelating agent |
| CN104322336A (en) * | 2014-09-29 | 2015-02-04 | 中国计量学院 | Method adopting sodium nitroprusside for controlling rice heavy metal mercury accumulation |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101704016A (en) * | 2009-11-17 | 2010-05-12 | 上海市农业科学院 | Phytochemically combined remediation technology for soil contaminated by heavy metals, Mn, Pb, Cr and Zn |
| CN103039150A (en) * | 2013-01-14 | 2013-04-17 | 中国林业科学研究院亚热带林业研究所 | Efficient low-price rapid abandoned tailing vegetation restoration method |
| CN103962367A (en) * | 2014-04-15 | 2014-08-06 | 西南科技大学 | Method for promoting enrichment of cadmium by red spinach through composition of plant hormone and chelating agent |
| CN104322336A (en) * | 2014-09-29 | 2015-02-04 | 中国计量学院 | Method adopting sodium nitroprusside for controlling rice heavy metal mercury accumulation |
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