CN105497318A - Biological agent used for enhancing immunity of organism and preparation method thereof - Google Patents

Biological agent used for enhancing immunity of organism and preparation method thereof Download PDF

Info

Publication number
CN105497318A
CN105497318A CN201511005999.XA CN201511005999A CN105497318A CN 105497318 A CN105497318 A CN 105497318A CN 201511005999 A CN201511005999 A CN 201511005999A CN 105497318 A CN105497318 A CN 105497318A
Authority
CN
China
Prior art keywords
preparation
weight proportion
culture medium
concentrated
strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201511005999.XA
Other languages
Chinese (zh)
Inventor
江瀚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Probiotik Health Technology Co Ltd
Original Assignee
Shanghai Probiotik Health Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Probiotik Health Technology Co Ltd filed Critical Shanghai Probiotik Health Technology Co Ltd
Priority to CN201511005999.XA priority Critical patent/CN105497318A/en
Publication of CN105497318A publication Critical patent/CN105497318A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7016—Disaccharides, e.g. lactose, lactulose
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/745—Bifidobacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/062—Ascomycota
    • A61K36/064—Saccharomycetales, e.g. baker's yeast
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/08—Solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • A61K9/1617—Organic compounds, e.g. phospholipids, fats
    • A61K9/1623—Sugars or sugar alcohols, e.g. lactose; Derivatives thereof; Homeopathic globules
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment

Abstract

The invention provides a preparation method of a biological agent used for enhancing the immunity of the organism. The preparation method includes the steps that saccharomyces cerevisiae, lactobacillus plantarum and bifidobacterium are mixed to obtain mixed bacteria, and the mixed bacteria are cultured in a brown sugar water culture medium in an airtight mode till the pH is smaller than 3.60 and then continue to be cultured for 12-15 days to obtain complex bacteria; radix puerariae, fructus hippophae, grape seeds, brown sugar and water are mixed to obtain a complex culture medium; the complex bacteria are added into the complex culture medium to be cultured for 20-25 days in an airtight mode to obtain a complex fermentation product; the complex fermentation product is further processed to the obtain the biological agent. According to the preparation method, as the active ingredients of plants are converted through the microorganism complex fermentation technology, the obtained biological agent has the advantages of being easier to absorb and utilize, significant in effect and the like, and besides, the biological agent can effectively achieve the beneficial effects of resisting oxidation, scavenging free radicals and enhancing the immunity and is a natural biological agent capable of enhancing the immunity of the organism.

Description

A kind of biological preparation for enhancing human body immunity power and preparation method thereof
Technical field
The present invention relates to field of health care food, be specifically related to a kind of biological preparation, particularly relate to a kind of biological preparation for enhancing human body immunity power and preparation method thereof.
Background technology
Immunity is the defense mechanism of human body self, there is any foreign body (virus, antibacterial etc.) identifying and eliminate exotic invasive, the own cells of process aging, damage, death, degeneration, and identify and the ability of mutant cell and virus infected cell in handling body, be the physiological reaction of a kind of human bioequivalence and eliminating " dissident ".At present, the impact that the immune system producing immunity can be subject in various degree due to the factor of each fermentation, and then affect the immunity level of body, such as: the harmful substance in our ambient contamination can make human body produce the free radical of cell of impairing one's health, and these free radicals can destroy our immune system thus cause harm in various degree to human body self; Vitamin B complexes a large amount of in the bad habit such as smoking, excessive consumption of alcohol meeting consumer, vitamin C (1 cigarette combustion produces 30,000 hundred million free radicals, consumes 25mg vitamin C), thus make the immunity degradation of health; Live irregular, sufficient sleep can not be ensured, the immune system of health also can be made to come to harm; In addition, the physical training of immoderation, as excessive movement the free radical that produces also can have lethal effect to human body; Pressure, spirit depressing, chronic anxiety can weaken the ability of immune system fight disease equally; And to injure maximum factor to immune system be that nutrition is unbalanced.
When natural immune system come to harm make hypoimmunity time, the most directly performance be exactly liable to illness.And the often ill consumption that can increase the weight of body, thus be attended by have a delicate constitution, the reduction of malnutrition, lethargy, fatigue and weak, appetite, the performance such as sleep disorder, therefore, sick, have an injection, take medicine the normal potluck that just gets married.Moreover, the at every turn sick time also can be especially very long, even if temporarily get well also can recurrent exerbation again for body, if things go on like this, easily brings out major disease.
Modern medicine study achievement shows, the materials such as the polysaccharide contained in a lot of Chinese herbal medicine, organic acid, alkaloids, glycoside and volatile oil, can across-the-board regulation and give full play to the immunologic function and adaptive faculty that animal body has, therefore, there is the effect of preventing and curing diseases.Wherein, CN101904500A discloses functional food of a kind of enhancing immunity and preparation method thereof, CN102783645A discloses a kind of health product for improving immunity, CN102551067A discloses a kind of oral liquid prescription of immunity moderation power, in these prior aries, all developed by multiple Chinese herbal medicine or its extract, and need through to concoct or after processing, further effective component extracting, there is remarkable result the side of taking, but often this process very complicated; And if directly take without processing, then the obstacle owing to absorbing, cause effective ingredient not easily to utilize, the defect of DeGrain.
Summary of the invention
Based on above-mentioned background technology, the invention provides a kind of biological preparation for enhancing human body immunity power and preparation method thereof, transformed by the effective ingredient of microorganism composite fermentation technology to plant, the biological preparation obtained is had more easily absorb and utilize, the feature such as Be very effective, moreover, effectively can also realize the beneficial effect of antioxidation, scavenging free radicals, enhancing immunity, be a kind of can the natural biological preparation of enhancing human body immunity power.
Technical scheme of the present invention comprises a kind of preparation method of the biological preparation for enhancing human body immunity power, it is characterized in that, comprising:
Step 1: be mixed to get mixed vaccine according to the weight proportion of saccharomyces cerevisiae 10-20 part, Lactobacillus plantarum 20-30 part, bacillus bifidus 35-50 part, added in brown sugar water culture medium, under 32-35 DEG C of condition, the airtight pH of being cultured to is less than 3.60, then continue to cultivate 12-15 days, obtain compound bacteria;
Step 2: be mixed to get complex medium according to the weight proportion of Radix Puerariae 20-30 part, Fructus Hippophae 15-25 part, Semen Vitis viniferae 10-15 part, brown sugar 10-15 part, water 40-60 part;
Step 3: described compound bacteria is added in described complex medium, under 30-38 DEG C of condition, airtight cultivation 20-25 days, obtains composite fermentation thing;
Step 4: described composite fermentation thing is soaked, drip washing, concentrated, sterilizing, molding, obtain described biological preparation.
In one embodiment of the invention, wherein:
Described saccharomyces cerevisiae (Saccharomycescerevisiae) for deposit number be the strain of ACCC20065;
Described Lactobacillus plantarum (Lactobacillusplantarum) for deposit number be the strain of ACCC11118;
Described bacillus bifidus (Bifidobacterium) for deposit number be the strain of ACCC11054.
In a preferred embodiment of the invention, described preparation method also comprises the cultivation of strain, is specially:
Carry out shaken cultivation by saccharomyces cerevisiae strain access Rhizoma Solani tuber osi fluid medium, wherein, cultivation temperature is 28-32 DEG C, the revolution of shaken cultivation is 180-220rpm, duration of oscillation is 48-72 hour, takes out after culture medium muddiness, puts into 2-4 DEG C of Refrigerator store stand-by;
Lactobacillus plantarum strain, bifidobacterium species are accessed respectively in MRS fluid medium and carry out quiescent culture, wherein, cultivation temperature is 35-39 DEG C, and incubation time is 48-72 hour, takes out after culture medium muddiness, puts into 2-4 DEG C of Refrigerator store stand-by.
In one embodiment, about step 1, preferably, described mixed vaccine is added in brown sugar water culture medium according to the weight proportion of 1:80-120, and wherein, the component of described brown sugar water culture medium and weight proportion thereof are brown sugar 5-10 part, water 90-98 part.
In another embodiment, about step 2, preferably, described Radix Puerariae, Fructus Hippophae, Semen Vitis viniferae are pulverized by pulverizer, obtain 30-50 order granule.
In one embodiment, preferably, about step 3, described compound bacteria is added in described complex medium according to the weight proportion of 1:15-20.
In yet another embodiment, preferably, about step 4, wherein:
By adding in described composite fermentation thing, to add water to solid-liquid weight proportion be 1:1, soaks 1-2 days;
Then drip washing, and leacheate is concentrated into 20-30% under 40-50 DEG C of condition, obtain concentrated solution;
Further, sterilizing 10-30 minute under 60-70 DEG C of condition, obtains concentrated sterilized solution;
Described molding is the further course of processing, and wherein, described biological preparation can be solid formulation, can be also liquid formulation, as oral liquid;
Wherein, procedure of processing further about solid formulation is: according to concentrated sterilized solution 15-30 part, erythritol 15-30 part, lactose 30-60 part, the weight proportion of protective agent 3-5 part takes, first described erythritol and lactose are added in spraying dry one-step-granulating method successively, then start to spray into described concentrated sterilized solution, flow is 10-20ml/min, finally spray into the protective agent be dissolved in the water, wherein, the inlet temperature of described granulator is 60-80 DEG C, leaving air temp is 40-60 DEG C, discharging when temperature drops to 25-35 DEG C in day with fog to be painted, obtain described solid formulation,
Wherein, the procedure of processing further of described oral liquid is: take according to the weight proportion of concentrated sterilized solution 45-50 part, erythritol 45-50 part, protective agent 5-10 part; first dissolve adding appropriate hot water stirs in described erythritol and protective agent; to be cooled to after 25-35 DEG C; add described concentrated sterilized solution; stir to clarify, obtain described oral liquid.
Technical scheme of the present invention also comprises the described biological preparation that a kind of preparation method described above prepares, for enhancing human body immunity power.
The present invention adopts country to defend strain in " can be used for the strain list of food " of planning commission's bulletin, adopts conventional plant and fruit to be raw material, carries out composite fermentation and prepare a kind of natural biological preparation.The present invention combines the effective ingredient of the first kind in existing health product and Equations of The Second Kind, the active component of a large amount of oxidation resistance is rich in product, such as: flavonoid (4.73%), soaping agents (86.4mg/100g), aminoacid (4.01%), glutathion (886mg/100g), vitamin C (322mg/100g), vitamin E (908 μ g/100g), SOD (194000U/100g), lactate (5.04%) and various trace elements (ferrum: 503mg/kg; Selenium: 0.18mg/kg etc.) etc.
Technical scheme of the present invention has the function possessing enhancing immunity, transformed by the effective ingredient of microorganism composite fermentation technology to plant, make it have and more easily absorb, the features such as Be very effective, be a kind of can the natural biological preparation of enhancing immunity.
Detailed description of the invention
The invention provides a kind of preparation method of the biological preparation for enhancing human body immunity power, it is characterized in that, comprising:
Step 1: be mixed to get mixed vaccine according to the weight proportion of saccharomyces cerevisiae 10-20 part, Lactobacillus plantarum 20-30 part, bacillus bifidus 35-50 part, added in brown sugar water culture medium, under 32-35 DEG C of condition, the airtight pH of being cultured to is less than 3.60, then continue to cultivate 12-15 days, obtain compound bacteria;
Step 2: be mixed to get complex medium according to the weight proportion of Radix Puerariae 20-30 part, Fructus Hippophae 15-25 part, Semen Vitis viniferae 10-15 part, brown sugar 10-15 part, water 40-60 part;
Step 3: described compound bacteria is added in described complex medium, under 30-38 DEG C of condition, airtight cultivation 20-25 days, obtains composite fermentation thing;
Step 4: described composite fermentation thing is soaked, drip washing, concentrated, sterilizing, molding, obtain described biological preparation.
In one embodiment of the invention, wherein:
Described saccharomyces cerevisiae (Saccharomycescerevisiae) for deposit number be the strain of ACCC20065;
Described Lactobacillus plantarum (Lactobacillusplantarum) for deposit number be the strain of ACCC11118;
Described bacillus bifidus (Bifidobacterium) for deposit number be the strain of ACCC11054.
In a preferred embodiment of the invention, described preparation method also comprises the cultivation of strain, is specially:
Carry out shaken cultivation by saccharomyces cerevisiae strain access Rhizoma Solani tuber osi fluid medium, wherein, cultivation temperature is 28-32 DEG C, the revolution of shaken cultivation is 180-220rpm, duration of oscillation is 48-72 hour, takes out after culture medium muddiness, puts into 2-4 DEG C of Refrigerator store stand-by;
Lactobacillus plantarum strain, bifidobacterium species are accessed respectively in MRS fluid medium and carry out quiescent culture, wherein, cultivation temperature is 35-39 DEG C, and incubation time is 48-72 hour, takes out after culture medium muddiness, puts into 2-4 DEG C of Refrigerator store stand-by.
In one embodiment, about step 1, preferably, described mixed vaccine is added in brown sugar water culture medium according to the weight proportion of 1:80-120, and wherein, the component of described brown sugar water culture medium and weight proportion thereof are brown sugar 5-10 part, water 90-98 part.
In another embodiment, about step 2, preferably, described Radix Puerariae, Fructus Hippophae, Semen Vitis viniferae are pulverized by pulverizer, obtain 30-50 order granule.
In one embodiment, preferably, about step 3, described compound bacteria is added in described complex medium according to the weight proportion of 1:15-20.
In yet another embodiment, preferably, about step 4, wherein:
By adding in described composite fermentation thing, to add water to solid-liquid weight proportion be 1:1, soaks 1-2 days;
Then drip washing, and leacheate is concentrated into 20-30% under 40-50 DEG C of condition, obtain concentrated solution;
Further, sterilizing 10-30 minute under 60-70 DEG C of condition, obtains concentrated sterilized solution;
Described molding is the further course of processing, and wherein, described biological preparation can be solid formulation, can be also liquid formulation, as oral liquid;
Wherein, procedure of processing further about solid formulation is: according to concentrated sterilized solution 15-30 part, erythritol 15-30 part, lactose 30-60 part, the weight proportion of protective agent 3-5 part takes, first described erythritol and lactose are added in spraying dry one-step-granulating method successively, then start to spray into described concentrated sterilized solution, flow is 10-20ml/min, finally spray into the protective agent be dissolved in the water, wherein, the inlet temperature of described granulator is 60-80 DEG C, leaving air temp is 40-60 DEG C, discharging when temperature drops to 25-35 DEG C in day with fog to be painted, obtain described solid formulation,
Wherein, the procedure of processing further of described oral liquid is: take according to the weight proportion of concentrated sterilized solution 45-50 part, erythritol 45-50 part, protective agent 5-10 part; first dissolve adding appropriate hot water stirs in described erythritol and protective agent; to be cooled to after 25-35 DEG C; add described concentrated sterilized solution; stir to clarify, obtain described oral liquid.
Present invention also offers the described biological preparation that a kind of preparation method described above prepares, for enhancing human body immunity power.
Wherein, it should be noted that when not having specified otherwise, the content that the present invention relates to or proportioning refer to weight content or weight proportion.
According to technique scheme of the present invention, existing by following examples content of the present invention explained further and illustrate.
Embodiment 1
For a preparation method for the biological preparation of enhancing human body immunity power, comprising:
The first step: the cultivation of strain: by the saccharomyces cerevisiae strain (Saccharomycescerevisiae bought from Chinese agriculture Microbiological Culture Collection administrative center, ACCC20065) access in the conical flask of Rhizoma Solani tuber osi fluid medium, at 28 DEG C, 180-220rpm shaken cultivation 48 hours, takes out after culture medium muddiness; By Lactobacillus plantarum strain (Lactobacillusplantarum, ACCC11118), bifidobacterium species (Bifidobacterium, ACCC11054), access respectively in the conical flask of MRS fluid medium, cultivate 48 hours under 37 DEG C of conditions, take out after culture medium muddiness, then put into 4 DEG C of Refrigerator stores stand-by;
Second step: be mixed to get mixed vaccine according to the weight proportion of saccharomyces cerevisiae 15 parts, Lactobacillus plantarum 30 parts, bacillus bifidus 45 parts, added in brown sugar water culture medium according to the part by weight of 1:100, wherein, 5 parts, brown sugar, pure water 95 parts, after fermentation tank stirs, under 35 DEG C of conditions, the airtight pH of being cultured to is less than 3.60, then continue cultivation 15 days, obtain compound bacteria;
3rd step: filter out individual uniform Radix Puerariae, Fructus Hippophae, Semen Vitis viniferae, and be ground into 40 orders respectively by pulverizer, be mixed to get complex medium according to the weight proportion of Radix Puerariae 25 parts, Fructus Hippophae 20 parts, Semen Vitis viniferae 10 parts, 12 parts, brown sugar, 45 parts, water;
4th step: described compound bacteria is added in described complex medium by the part by weight of 1:20, after fermentation tank stirs, airtight cultivation 22 days under 32 DEG C of conditions, obtains composite fermentation thing;
5th step: add water in fermentation tank, make solid-to-liquid ratio reach 1:1, soak 1 day, then drip washing; Leacheate carries out being concentrated into 20% at 50 DEG C; After concentrated solution sterilizes 20 minutes at 60 DEG C, 4 DEG C of storages are stand-by;
6th step: the preparation of solid dosage: take according to the weight proportion of concentrated sterilized solution 20 parts, erythritol 20 parts, lactose 55 parts, protective agent 5 parts; first described erythritol and lactose are added in spraying dry one-step-granulating method successively; then start to spray into described concentrated sterilized solution; flow is 10ml/min; finally spray into the protective agent be dissolved in suitable quantity of water; wherein; the inlet temperature of described granulator is 60 DEG C; leaving air temp is 45 DEG C; discharging when temperature drops to 30 DEG C in day with fog to be painted, obtains described solid formulation.
Embodiment 2
For a preparation method for the biological preparation of enhancing human body immunity power, comprising:
The first step: the cultivation of strain: by the saccharomyces cerevisiae strain (Saccharomycescerevisiae bought from Chinese agriculture Microbiological Culture Collection administrative center, ACCC20065) access in the conical flask of Rhizoma Solani tuber osi fluid medium, at 28 DEG C, 180-220rpm shaken cultivation 48 hours, takes out after culture medium muddiness; By Lactobacillus plantarum strain (Lactobacillusplantarum, ACCC11118), bifidobacterium species (Bifidobacterium, ACCC11054), access respectively in the conical flask of MRS fluid medium, cultivate 48 hours under 37 DEG C of conditions, take out after culture medium muddiness, then put into 4 DEG C of Refrigerator stores stand-by;
Second step: be mixed to get mixed vaccine according to the weight proportion of saccharomyces cerevisiae 10 parts, Lactobacillus plantarum 30 parts, bacillus bifidus 50 parts, added in brown sugar water culture medium according to the part by weight of 1:100, wherein, 3 parts, brown sugar, pure water 97 parts, after fermentation tank stirs, under 33 DEG C of conditions, the airtight pH of being cultured to is less than 3.50, then continue cultivation 12 days, obtain compound bacteria;
3rd step: filter out individual uniform Radix Puerariae, Fructus Hippophae, Semen Vitis viniferae, and be ground into 40 orders respectively by pulverizer, be mixed to get complex medium according to the weight proportion of Radix Puerariae 30 parts, Fructus Hippophae 20 parts, Semen Vitis viniferae 12 parts, 10 parts, brown sugar, 40 parts, water;
4th step: described compound bacteria is added in described complex medium by the part by weight of 1:15, after fermentation tank stirs, airtight cultivation 25 days under 35 DEG C of conditions, obtains composite fermentation thing;
5th step: add water in fermentation tank, make solid-to-liquid ratio reach 1:1, soak 1 day, then drip washing; Leacheate carries out being concentrated into 20% at 45 DEG C; After concentrated solution sterilizes 10 minutes at 65 DEG C, 4 DEG C of storages are stand-by;
6th step: the preparation of oral liquid: take according to the weight proportion of concentrated sterilized solution 45 parts, erythritol 45 parts, protective agent 10 parts; first will add appropriate hot distilled water stirring and dissolving in described erythritol and protective agent; to be cooled to after 35 DEG C; add described concentrated sterilized solution; stir to clarify, obtain described oral liquid.
Detect embodiment
Animal divides into groups: select 90 healthy cleaning grade Kunming mouses, body weight 18-22g, after conventional adaptation feeds 2 days, be divided at random 3 groups (matched group, microbial source antioxidant group and composite antioxidant groups).
Experimental technique: test mice is fed basal diet, by table 1 gavage microbial source antioxidant and composite antioxidant, every day gavage once, gavage capacity is the distilled water of 0.2ml/10gbw, the capacity such as matched group filling, continuous gavage 30 days.Weigh a Mouse Weight weekly, adjustment gavage amount.
Table 1 mouse stomach dosage
The collection of sample and preparation:
1, the preparation of serum
Gavage is fasting 12h after 30 days, at 31d, takes a blood sample to pluck eyeball method, and low-temperature centrifugation after leaving standstill, gets supernatant sealing and be placed on preservation in cryogenic refrigerator, to be measured;
2, the collection of immune organ
31d, mice after blood sampling adopts cervical dislocation to put to death, rapid taking-up spleen and thymus, and the fat of rejecting around internal organs and connective tissue, by electronic balance weighing spleen and thymus weight in wet base, in order to Computation immunity shoot formation, wherein, select some at random, aseptically take out spleen for each group, in order to measure the conversion ratio of splenocyte.
Indexs measure and method:
1, Immune Organs Index
Thymus index (g/kg)=thymus weight in wet base (g)/Mouse Weight (kg),
Index and spleen index (g/kg)=spleen weight in wet base (g)/Mouse Weight (kg);
2, lymhocyte transformation rate
With mtt assay and tetramethyl azo azoles salt trace enzyme reaction colorimetric method for determining lymhocyte transformation rate;
3, immunoglobulin content
Immunoglobulin in Serum A (IgA), IgM (IgM), immunoglobulin G (IgG) all adopt enzyme linked immunosorbent assay (ELISA) to measure;
4, cytokine content
In serum, the content of interleukin-2 (IL-2), interleukin-6 (IL-6) all adopts enzyme linked immunosorbent assay (ELISA) to measure;
5, date processing
Data Processing in Experiment adopts the one factor analysis of variance of SPSS17.0 with analyzing, result represents with mean+SD.
Experimental result is in table 2 and table 3:
Table 2 mouse immune shoot formation
Project Matched group Microbial source antioxidant group Composite antioxidant group
Thymus Thymus 3.644±0.704 3.909±0.807 4.232±0.618
Spleen Spleen 3.270±0.585 3.413±0.709 3.154±0.525
The change of mouse spleen lymphocyte conversion ratio, immunoglobulin content and cytokine levels that table 3ConA induces
Project Matched group Microbial source antioxidant group Composite antioxidant group
Splenic vein hemodynamics rate 0.331±0.028 0.374±0.023 0.367±0.021
IgA(μg/ml) 22.46±9.12 38.01±2.09 33.18±2.73
IgM(μg/ml) 6.26±4.52 9.80±1.55 9.00±3.31
IgG(μg/ml) 25.63±0.52 24.66±2.28 23.03±3.38
IL-2(ng/L) 23.24±7.09 37.04±11.00 42.94±7.22
IL-6(ng/L) 92.83±24.17 140.35±39.29 143.79±45.45
By upper Biao Ke get: from Immune Organs Index, lymhocyte transformation rate, immunoglobulin content, cytokine content, antioxidant can improve the immune function of body, shows that the present invention has the effect of good enhancing immunity.
Further, adopt same above-mentioned requirement of experiment, the microbial origin antioxidant in oxidant group is replaced with the biological preparation prepared in embodiment 1 or 2, finally detect and find that its These parameters is close with oxidant group, be even better than oxidant group.
Be described in detail specific embodiments of the invention above, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.

Claims (10)

1. for a preparation method for the biological preparation of enhancing human body immunity power, it is characterized in that, comprising:
Step 1: be mixed to get mixed vaccine according to the weight proportion of saccharomyces cerevisiae 10-20 part, Lactobacillus plantarum 20-30 part, bacillus bifidus 35-50 part, added in brown sugar water culture medium, under 32-35 DEG C of condition, the airtight pH of being cultured to is less than 3.6, then continues to cultivate 12-15 days, obtains compound bacteria;
Step 2: be mixed to get complex medium according to the weight proportion of Radix Puerariae 20-30 part, Fructus Hippophae 15-25 part, Semen Vitis viniferae 10-15 part, brown sugar 10-15 part, water 40-60 part;
Step 3: described compound bacteria is added in described complex medium, under 30-38 DEG C of condition, airtight cultivation 20-25 days, obtains composite fermentation thing;
Step 4: described composite fermentation thing is soaked, drip washing, concentrated, sterilizing, molding, obtain described biological preparation.
2. preparation method according to claim 1, is characterized in that, described saccharomyces cerevisiae (Saccharomycescerevisiae) for deposit number be the strain of ACCC20065; Described Lactobacillus plantarum (Lactobacillusplantarum) for deposit number be the strain of ACCC11118; Described bacillus bifidus (Bifidobacterium) for deposit number be the strain of ACCC11054.
3. preparation method according to claim 1, is characterized in that, also comprises the cultivation of strain, is specially:
Carry out shaken cultivation by saccharomyces cerevisiae strain access Rhizoma Solani tuber osi fluid medium, wherein, cultivation temperature is 28-32 DEG C, the revolution of shaken cultivation is 180-220rpm, duration of oscillation is 48-72 hour, takes out after culture medium muddiness, puts into 2-4 DEG C of Refrigerator store stand-by;
Lactobacillus plantarum strain, bifidobacterium species are accessed respectively in MRS fluid medium and carry out quiescent culture, wherein, cultivation temperature is 35-39 DEG C, and incubation time is 48-72 hour, takes out after culture medium muddiness, puts into 2-4 DEG C of Refrigerator store stand-by.
4. preparation method according to claim 1, it is characterized in that, about step 1, described mixed vaccine is added in brown sugar water culture medium according to the weight proportion of 1:80-120, wherein, the component of described brown sugar water culture medium and weight proportion thereof are brown sugar 5-10 part, water 90-98 part.
5. preparation method according to claim 1, is characterized in that, about step 2, described Radix Puerariae, Fructus Hippophae, Semen Vitis viniferae are pulverized by pulverizer, obtains 30-50 order granule.
6. preparation method according to claim 1, is characterized in that, about step 3, described compound bacteria is added in described complex medium according to the weight proportion of 1:15-20.
7. preparation method according to claim 1, is characterized in that, about step 4, wherein:
By adding in described composite fermentation thing, to add water to solid-liquid weight proportion be 1:1, soaks 1-2 days;
Then drip washing, and leacheate is concentrated into 20-30% under 40-50 DEG C of condition, obtain concentrated solution;
Further, sterilizing 10-30 minute under 60-70 DEG C of condition, obtains concentrated sterilized solution.
8. preparation method according to claim 1, it is characterized in that, described molding is the further course of processing, wherein, described biological preparation is solid formulation, procedure of processing further then about solid formulation is: according to concentrated sterilized solution 15-30 part, erythritol 15-30 part, lactose 30-60 part, the weight proportion of protective agent 3-5 part takes, first described erythritol and lactose are added in spraying dry one-step-granulating method successively, then start to spray into described concentrated sterilized solution, flow is 10-20ml/min, finally spray into the protective agent be dissolved in the water, wherein, the inlet temperature of described granulator is 60-80 DEG C, leaving air temp is 40-60 DEG C, discharging when temperature drops to 25-35 DEG C in day with fog to be painted, obtain described solid formulation.
9. preparation method according to claim 1; it is characterized in that; described biological preparation is liquid formulation; as oral liquid, then the procedure of processing further of described oral liquid is: take according to the weight proportion of concentrated sterilized solution 45-50 part, erythritol 45-50 part, protective agent 5-10 part, first dissolves adding appropriate hot water stirs in described erythritol and protective agent; to be cooled to after 25-35 DEG C; add described concentrated sterilized solution, stir to clarify, obtain described oral liquid.
10. the biological preparation prepared as the preparation method in claim 1-9 as described in any one.
CN201511005999.XA 2015-12-28 2015-12-28 Biological agent used for enhancing immunity of organism and preparation method thereof Pending CN105497318A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201511005999.XA CN105497318A (en) 2015-12-28 2015-12-28 Biological agent used for enhancing immunity of organism and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201511005999.XA CN105497318A (en) 2015-12-28 2015-12-28 Biological agent used for enhancing immunity of organism and preparation method thereof

Publications (1)

Publication Number Publication Date
CN105497318A true CN105497318A (en) 2016-04-20

Family

ID=55705877

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201511005999.XA Pending CN105497318A (en) 2015-12-28 2015-12-28 Biological agent used for enhancing immunity of organism and preparation method thereof

Country Status (1)

Country Link
CN (1) CN105497318A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106962724A (en) * 2017-03-17 2017-07-21 赵玉良 A kind of production technology of the probiotics solid beverage without chemical addition agent
CN110314205A (en) * 2019-05-30 2019-10-11 上海普乐拜格健康科技有限公司 A kind of cultured fishes hepatic injury reparation natural biological preparation and preparation method thereof
CN110464763A (en) * 2019-08-30 2019-11-19 海南木辣达生物科技有限公司 A kind of antipruritic antibacterial Moringa external application object and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101690743A (en) * 2009-09-30 2010-04-07 北京大北农动物保健科技有限责任公司 Traditional Chinese medicine biological agent for improving immunity of livestock and preparation method thereof
CN104623122A (en) * 2015-01-12 2015-05-20 江瀚生物科技（上海）有限公司 Biological preparation with anti-fatigue function and preparation method of biological preparation
CN104971111A (en) * 2014-04-14 2015-10-14 北京大北农动物保健科技有限责任公司 Traditional Chinese medicine bio-preparation of improving immunity of livestock and poultry, preparation method thereof and feed therewith

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101690743A (en) * 2009-09-30 2010-04-07 北京大北农动物保健科技有限责任公司 Traditional Chinese medicine biological agent for improving immunity of livestock and preparation method thereof
CN104971111A (en) * 2014-04-14 2015-10-14 北京大北农动物保健科技有限责任公司 Traditional Chinese medicine bio-preparation of improving immunity of livestock and poultry, preparation method thereof and feed therewith
CN104623122A (en) * 2015-01-12 2015-05-20 江瀚生物科技（上海）有限公司 Biological preparation with anti-fatigue function and preparation method of biological preparation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
金旭丹: "《神奇的元素》", 31 January 2007, 内蒙古人民出版社 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106962724A (en) * 2017-03-17 2017-07-21 赵玉良 A kind of production technology of the probiotics solid beverage without chemical addition agent
CN110314205A (en) * 2019-05-30 2019-10-11 上海普乐拜格健康科技有限公司 A kind of cultured fishes hepatic injury reparation natural biological preparation and preparation method thereof
CN110464763A (en) * 2019-08-30 2019-11-19 海南木辣达生物科技有限公司 A kind of antipruritic antibacterial Moringa external application object and preparation method thereof
CN110464763B (en) * 2019-08-30 2021-08-27 海南木辣达生物科技有限公司 Itching-relieving and bacteriostatic moringa oleifera external application and preparation method thereof

Similar Documents

Publication Publication Date Title
CN105087423B (en) Novel Bei Laisi bacillus CJBV and the bactericidal composition containing it
CN111406856B (en) Functional fermented beverage for dispelling effects of alcohol and protecting liver and preparation method thereof
CN104642771A (en) Application of Chinese herbal medicine feed additive to livestock and poultry feed
CN104585489A (en) An herbal dry-mixed feed and a preparation method thereof
CN104543384A (en) Chinese herbal medicine feed additive
CN105166899A (en) Fragrance flavored fruit and vegetable enzyme powder preparation method
CN104623122A (en) Biological preparation with anti-fatigue function and preparation method of biological preparation
CN105497318A (en) Biological agent used for enhancing immunity of organism and preparation method thereof
CN106190912A (en) Photosynthetic bacteria microbial inoculum and preparation method and application
CN114191464B (en) Traditional Chinese medicine microecological preparation for improving duck growth performance and application thereof
CN102204646A (en) Feed for fattening adult pigs
CN105076830A (en) Compound feed for channa maculate and preparation method of compound feed
CN106754427B (en) Penicillium lanophorum Kong Junxin strain and domestication method thereof
CN103931665B (en) A kind of mixing formula preparation preventing and treating brown planthopper
CN102851334B (en) Fermentation medium and fermentation method of aflatoxin B1
Ammar et al. Nutritive value of ajuga iva as a pastoral plant for ruminants: plant phytochemicals and in vitro gas production and digestibility
CN104605149A (en) Chinese herbal medicine mixed fermentation feed
TW200948371A (en) Cultivation method and liquid culture medium for cultivation of mycelium and fruiting body of Cordyceps
CN103975957B (en) A kind of mixing formula formulation preparation method preventing and treating brown planthopper
CN105535282A (en) Biological agent for irritable infertility function repair and preparation method thereof
CN105558350A (en) Feed containing Chinese herbal medicine compound crude polysaccharide and preparation method of feed
CN106190913A (en) One strain Rhodopseudomonas palustris and store method thereof
CN103947684A (en) Application of mixed formula preparation in prevention and treatment of brown planthopper
Alghamdi Nutritional value and phytochemical constituents of some plantago spp. of ha‟ il region, Saudi Arabia
CN103550286B (en) A kind of infectious bursal disease live-vaccine effervescent tablet immunological adjuvant

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20160420