CN105493916A - Method for reducing content of nicotine in flue-cured tobacco leaves - Google Patents

Method for reducing content of nicotine in flue-cured tobacco leaves Download PDF

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Publication number
CN105493916A
CN105493916A CN201510876945.4A CN201510876945A CN105493916A CN 105493916 A CN105493916 A CN 105493916A CN 201510876945 A CN201510876945 A CN 201510876945A CN 105493916 A CN105493916 A CN 105493916A
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flue
vitamin
nicotine
cured tobacco
glucose oxidase
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CN201510876945.4A
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Inventor
张一扬
方丽婷
李鑫
李强
张毅
周冀衡
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Hunan Agricultural University
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Hunan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/30Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Forests & Forestry (AREA)
  • Ecology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Toxicology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention provides a method for reducing the content of nicotine in flue-cured tobacco leaves. Within two hours after flue-cured tobaccos are topped, the topped sections of the flue-cured tobaccos are smeared with 1.0-2.0 ml of a nicotine signal regulating substance solution, and covered with round paper patches with the diameter being 2.5 cm; according to the using method, 1, the nicotine signal regulating substance solution is used instantly after being prepared, wherein 1000 ml of purified water is mixed with 8.0-10.0 g of glucose oxidase, 2.0-3.0 g of vitamin C, 3.0-5.0 g of vitamin E, 300-500 mg of vitamin E, 5 g of sorbitol, 50 g of glycerol and 10 g of magnesium sulfate heptahydrate; according to the different soil types, flue-cured tobacco varieties and the nitrogen fertilizer application level, the different smearing amounts of the nicotine signal regulating substance solution are determined. According to the method for reducing the content of the nicotine in the flue-cured tobacco leaves, the synthetic amount of the nicotine in flue-cured tobacco roots can be reduced, the content of the nicotine in the tobacco leaves of all parts is reduced, especially, the content of nicotine in the tobacco leaves of the upper portion is reduced, coordination of chemical ingredients of the tobacco leaves is improved, industrial usability of the tobacco leaves is improved, and the method is particularly suitable for being applied and popularized in high-grade cigarette brand base units.

Description

A kind of method reducing Nicotine Content in Flue-cured Tobacco
Technical field
The present invention relates to the production and application after adopting antioxidant semiochemicals to pinch to flue-cured tobacco, particularly relate to a kind of method reducing Nicotine Content in Flue-cured Tobacco.
Background technology
China's flue-cured tobacco is due to after farming operation measure of pinching, cigarette strain root system nicotine synthesis capability significantly improves, the tobacco leaf nicotine of different parts distributes and accumulation increases sharply, especially upper tobacco leaf nicotine content is higher, tobacco leaf intrinsic chemical composition harmony is caused to be deteriorated, tobacco leaf sensory evaluating smoking quality excitant strengthens, and tobacco leaf usability is deteriorated.Under the background of China's Tar, reduce nicotine content of tobacco leaves, especially reduce upper tobacco leaf nicotine content, become the important restrictive factor that restriction raw tobacco material is produced.
The method of existing reduction nicotine content of tobacco leaves, the general usage amount adopting minimizing nitrogenous fertilizer, reduce number of times and the height of cultivation and banking, or adopt stays wooden fork to stay the methods such as leaf more, although this kind of method can reduce nicotine content of tobacco leaves to a certain extent, but simultaneously significantly reducing tobacco leaf interior quality and economic flow rate, is not especially obvious especially to reduction upper tobacco leaf nicotine content effect yet.By hormone regulating and controlling, also can reduce nicotine content of tobacco leaves, but tobacco leaf thickens, flammability is poor, and sensory evaluating smoking's difficult quality is accepted by cigarette enterprise.Therefore, it may be necessary research, seek one and can coordinate tobacco leaf intrinsic chemical composition, a kind ofly can reduce again the method for Nicotine Content in Flue-cured Tobacco.
Summary of the invention
Technical problem to be solved by this invention is the defect making up above-mentioned prior art, there is provided a kind of cost low, operation is implemented simple and easy, slow down the problem of the root system nicotine synthesis acceleration that mechanical damage signal of pinching causes, coordinate tobacco leaf intrinsic chemical composition, reduce nicotine content of tobacco leaves, promote a kind of method of cured tobacco leaf quality.
Technical problem of the present invention is solved by the following technical programs.
A kind of feature reducing the method for Nicotine Content in Flue-cured Tobacco of the present invention is.
Described nicotine signals-modulating substance solution is used and collocation method: flue-cured tobacco is pinched in latter 2 hours, smears 1.0 ~ 2.0ml nicotine signals-modulating substance solution at the tangent plane place that flue-cured tobacco is pinched, and covers the round paper paster of diameter 2.5cm; Described nicotine signals-modulating substance solution is now with the current; 1000ml pure water proportioning 8 ~ 10g glucose oxidase, 2 ~ 3g vitamin C, 300 ~ 500mg support one's family E, sorbierite 5g, glycerine 50g, epsom salt 10g.
Nicotine signals-modulating substance solution may be used for isolating flue-cured tobacco pinch after the contact of oxygen in the surface of a wound and air, reduce flue-cured tobacco to the stress reaction of mechanical wounding, by anti-oxidant intracellular signaling to root system, reduce the synthetic amount of flue-cured tobacco root system nicotine, reduce each position nicotine content of tobacco leaves, especially upper tobacco leaf nicotine content, thus improve the harmony of tobacco leaf chemical composition, the industrial applicability of tobacco leaf is improved, is especially suitable for applying at high-end cigarette brand base unit.
The every strain applying amount of described glucose oxidase is 0.8 ~ 1.0mg, activity >=100,000 u/g; Effectively can reduce the synthetic amount of root system nicotine in described glucose oxidase finite concentration, suppress distribution and the cumulant of each position tobacco leaf nicotine simultaneously.
Described vitamin C adopts medical oral tablet, according to 0.1 gram/piece of actual usage amount of conversion; Vitamin C is rapid deterioration in aqueous, is strong reductant, reduces rapidly the oxidative damage of cigarette strain wound after pinching.
Described vitamin E adopts medical oral tablet, converts actual usage amount according to 10mg/ sheet; Described vitamin E is a kind of lipovitamin, and its hydrolysate is vitamin e, is topmost antioxidant, has stronger dissolubility, and then play antioxidation to the lipid material of cigarette strain surface of a wound secretion after pinching.
The configuration of described sorbierite, glycerine, epsom salt is the fat-soluble emulsifier of a configuration aqueous phase, is conducive to oxygen in air-isolation and contacts with wound, also helps dissolving and the emulsification of vitamin E.
The inventive method beneficial effect is compared with prior art.
The inventive method can not reduce yield of tobacco; Reduce thickness and the elasticity of tobacco leaf, occur that flammability is deteriorated, the problems such as excitant enhancing, obviously will reduce nicotine content of tobacco leaves, on the basis of especially upper tobacco leaf nicotine content, tobacco leaf intrinsic chemical composition harmony is strengthened, and aesthetic quality is obviously promoted, rational tobacco leaf production value can also be kept simultaneously, too much Productive statistics cost can not be increased.
Embodiment
According to the difference of different soil typess, flue-cured tobacco cultivars and nitrogen amount applied, the proportioning of nicotine signals-modulating substance solution and the amount of application of the every strain of flue-cured tobacco (emulsifier ratio of wherein respectively filling a prescription is constant, sees claim 1) specific as follows.
Embodiment 1.
Red soil, cloud and mist 87, when executing purity nitrogen 6.0kg, 1000ml pure water proportioning 8.0g glucose oxidase, 2.0g vitamin C, 400.0mg support one's family E.
Embodiment 2.
Red soil, cloud and mist 87, when executing purity nitrogen 9.0kg, 1000ml pure water proportioning 10.0g glucose oxidase, 3.0g vitamin C, 400.0mg support one's family E.
Embodiment 3.
Red soil, K326, when executing purity nitrogen 6.0kg, 1000ml pure water proportioning 9.0g glucose oxidase, 2.0g vitamin C, 500.0mg support one's family E.
Embodiment 4.
Red soil, K326, when executing purity nitrogen 9.0kg, 1000ml pure water proportioning 10.0g glucose oxidase, 3.0g vitamin C, 500.0mg support one's family E.
Embodiment 5.
Yellow earth, cloud and mist 87, when executing purity nitrogen 6.0kg, 1000ml pure water proportioning 8.0g glucose oxidase, 2.0g vitamin C, 300.0mg support one's family E.
Embodiment 6.
Yellow earth, cloud and mist 87, when executing purity nitrogen 9.0kg, 1000ml pure water proportioning 9.5g glucose oxidase, 3.0g vitamin C, 300.0mg support one's family E.
Embodiment 7.
Yellow earth, K326, when executing purity nitrogen 6.0kg, 1000ml pure water proportioning 8.5g glucose oxidase, 2.0g vitamin C, 400.0mg support one's family E.
Embodiment 8.
Yellow earth, K326, when executing purity nitrogen 9kg, 1000ml pure water proportioning 9.0g glucose oxidase, 3.0g vitamin C, 400.0mg support one's family E.
Nicotine signals-modulating substance solution configuration and use operation item: described glucose oxidase, vitamin C, vitamin E, should fully to pulverize, after shaking up with emulsifier such as sorbierite, glycerine, epsom salts, to make every effort to after pinching within 2 hours uniform application on the surface of a wound, and cover upper circular paper.

Claims (7)

1. reduce a method for Nicotine Content in Flue-cured Tobacco, it is characterized in that: flue-cured tobacco is pinched in latter 2 hours, smear 1.0 ~ 2.0ml nicotine signals-modulating substance solution at the tangent plane place that flue-cured tobacco is pinched, and cover the round paper paster of diameter 2.5cm; Described nicotine signals-modulating substance solution is now with the current; 1000ml pure water proportioning 8.0 ~ 10.0g glucose oxidase, 2.0 ~ 3.0g vitamin C, 300 ~ 500mg support one's family E, sorbierite 5.0g, glycerine 50.0g, epsom salt 10.0g.
2. a kind of method reducing Nicotine Content in Flue-cured Tobacco as claimed in claim 1, is characterized in that:
The every strain applying amount of described glucose oxidase is 0.8 ~ 1.0mg, activity >=100,000 u/g.
3. a kind of method reducing Nicotine Content in Flue-cured Tobacco as claimed in claim 1, is characterized in that:
Described vitamin C adopts medical oral tablet, according to 0.1 gram/piece of actual usage amount of conversion.
4. a kind of method reducing Nicotine Content in Flue-cured Tobacco as claimed in claim 1, is characterized in that:
Described vitamin E adopts medical oral tablet, converts actual usage amount according to 10mg/ sheet.
5. a kind of method reducing Nicotine Content in Flue-cured Tobacco as claimed in claim 1, is characterized in that:
The configuration of described sorbierite, glycerine, epsom salt is the fat-soluble emulsifier of a configuration aqueous phase.
6. a kind of method reducing Nicotine Content in Flue-cured Tobacco according to claim 1, is characterized in that: using method is:
(1) according to the difference of different soil typess, flue-cured tobacco cultivars and nitrogen amount applied, the proportioning of nicotine signals-modulating substance solution and the amount of application of the every strain of flue-cured tobacco (emulsifier ratio of wherein respectively filling a prescription is constant, sees claim 1) specific as follows:
(2) red soil, cloud and mist 87, when executing purity nitrogen 6.0kg, 1000ml pure water proportioning 8.0g glucose oxidase, 2.0g vitamin C, 400.0mg support one's family E;
(3) red soil, cloud and mist 87, when executing purity nitrogen 9.0kg, 1000ml pure water proportioning 10.0g glucose oxidase, 3.0g vitamin C, 400.0mg support one's family E;
(4) red soil, K326, when executing purity nitrogen 6.0kg, 1000ml pure water proportioning 9.0g glucose oxidase, 2.0g vitamin C, 500.0mg support one's family E;
(5) red soil, K326, when executing purity nitrogen 9.0kg, 1000ml pure water proportioning 10.0g glucose oxidase, 3.0g vitamin C, 500.0mg support one's family E;
(6) yellow earth, cloud and mist 87, when executing purity nitrogen 6.0kg, 1000ml pure water proportioning 8.0g glucose oxidase, 2.0g vitamin C, 300.0mg support one's family E;
(7) yellow earth, cloud and mist 87, when executing purity nitrogen 9.0kg, 1000ml pure water proportioning 9.5g glucose oxidase, 3.0g vitamin C, 300.0mg support one's family E;
(8) yellow earth, K326, when executing purity nitrogen 6.0kg, 1000ml pure water proportioning 8.5g glucose oxidase, 2.0g vitamin C, 400.0mg support one's family E;
(9) yellow earth, K326, when executing purity nitrogen 9kg, 1000ml pure water proportioning 9.0g glucose oxidase, 3.0g vitamin C, 400.0mg support one's family E.
7. a kind of using method reducing the method for Nicotine Content in Flue-cured Tobacco according to claim 6, is characterized in that:
Described glucose oxidase, vitamin C, vitamin E, fully should to pulverize, after shaking up with the emulsifier such as sorbierite, glycerine, epsom salt, to make every effort to after pinching within 2 hours uniform application on the surface of a wound, and cover upper circular paper.
CN201510876945.4A 2015-12-03 2015-12-03 Method for reducing content of nicotine in flue-cured tobacco leaves Pending CN105493916A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106472138A (en) * 2016-10-09 2017-03-08 云南省烟草公司昆明市公司 A kind of optimization K326 Nicotiana tabacum L. chemical composition improves the management method of Flue-cured tobacco Quality
CN106577763A (en) * 2016-12-22 2017-04-26 四川省烟草公司达州市公司 Conditioner capable of reducing proportion of conversion plants of burley tobacco group and method for treating tobacco plant by conditioner
CN106576959A (en) * 2016-12-21 2017-04-26 贵州省烟草科学研究院 Topping method for flue-cured tobacco
CN109805439A (en) * 2019-01-16 2019-05-28 中国农业科学院烟草研究所 A kind of inorganic agent and processing method reducing flue-cured tobacco upper tobacco leaf nicotine content

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106472138A (en) * 2016-10-09 2017-03-08 云南省烟草公司昆明市公司 A kind of optimization K326 Nicotiana tabacum L. chemical composition improves the management method of Flue-cured tobacco Quality
CN106576959A (en) * 2016-12-21 2017-04-26 贵州省烟草科学研究院 Topping method for flue-cured tobacco
CN106577763A (en) * 2016-12-22 2017-04-26 四川省烟草公司达州市公司 Conditioner capable of reducing proportion of conversion plants of burley tobacco group and method for treating tobacco plant by conditioner
CN109805439A (en) * 2019-01-16 2019-05-28 中国农业科学院烟草研究所 A kind of inorganic agent and processing method reducing flue-cured tobacco upper tobacco leaf nicotine content
CN109805439B (en) * 2019-01-16 2021-07-30 中国农业科学院烟草研究所 Treating agent and treating method for reducing nicotine content of upper tobacco leaves of flue-cured tobaccos

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