CN105475230A - Method for improving fertility of older animals - Google Patents
Method for improving fertility of older animals Download PDFInfo
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- CN105475230A CN105475230A CN201510954821.3A CN201510954821A CN105475230A CN 105475230 A CN105475230 A CN 105475230A CN 201510954821 A CN201510954821 A CN 201510954821A CN 105475230 A CN105475230 A CN 105475230A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/194—Carboxylic acids, e.g. valproic acid having two or more carboxyl groups, e.g. succinic, maleic or phthalic acid
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Animal Behavior & Ethology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention relates to the field of animal husbandry and animal reproduction and particularly discloses a method for improving the fertility including the litter size and the sexual period breeding rate of older animals. It is found for the first time that the sexual period breeding rate and the litter size of multiparous animals can be remarkably increased by enabling the older animals to orally take a proper dose (the daily dose ranges from 0.05 g/kg to 1.58 g/kg) of alpha-ketoglutarate for a long time. The multiparous animals take alpha-ketoglutarate by adding alpha-ketoglutarate to drinking water or feed of the animals, adding according to requirements can be conveniently achieved, the dose can be changed in time, and the method can be widely used under an automatic feeding and water feeding system. The method is applied to livestock breeding, the edible safety of meat can be guaranteed, and meanwhile development of the breeding industry is also promoted.
Description
Technical field
The present invention relates to herding and animal reproduction field, specifically, relate to a kind of method improving above the average age for marriage animal reproduction power.
Background technology
The fertility improving animal is to greatest extent one of target of husbandry sector pursuit, is also the important channel of improving animal husbandry economy benefit.China is populous nation, very large to the demand of meat product.Wherein, pork is the main meat of China's consumption of resident.But owing to being subject to environment, nutrition, heredity, stress waiting the impact of factor, the nest litter size of market pig is lower.At present, China's year live pig amount of delivering for sale is approximately 600,000,000, and sow breeding stock is minimum at 4,100 ten thousand, an average sow total amount of livestock for sale 15.One of thinking of dealing with problems improves animal reproduction efficiency, as nest litter size.Therefore, seek a kind of method improving multiparous animal reproduction rate and become very important.
A-ketoglutaric acid (α-Ketoglutaricacid, α-KG) be very important cellular energy metabolism intermediate product, it and amino acid, sodium, potassium, calcium, the salifies such as magnesium can form series of products, can be used as the composition of sport nutrition beverage, is organic intermediate, the matched reagent of biochemical reagents.There are some researches show, α-ketoglutaric acid can alleviate grice diarrhoea, promote that body weight increases and skeleton development, improve insulin in blood plasma, Insulin-like growth factor II-1 growth, promote pancreas beta-secretase insulin, improve the level of BGP under stress situation, improve the negative nitrogen balance of postoperative patient and muscle alienation etc.In the recent period, have scholar to find, certain density α-ketoglutaric acid can extend the life-span of nematode.
Summary of the invention
In order to solve problems of the prior art, the object of this invention is to provide a kind of method improving above the average age for marriage animal reproduction power.
In order to realize the object of the invention, technical scheme of the present invention is as follows:
Improve a method for above the average age for marriage animal reproduction power, make animal absorb α-ketoglutaric acid by oral way.Inventor finds, enables multiparous animal oral suitable dose α-ketoglutaric acid improve litter size and the feelings phase breeding rate of above the average age for marriage animal significantly.
As preferably, the daily dose of animal consumption α-ketoglutaric acid is 0.05 ~ 1.58g/kg with the weight of animals ratio.Under the picked-up daily dose of this α-ketoglutaric acid, above the average age for marriage animal can improve litter size significantly.
More preferred, the daily dose of animal consumption α-ketoglutaric acid is 0.30g/kg with the weight of animals ratio.Under the picked-up daily dose of this α-ketoglutaric acid, improve the best results of above the average age for marriage animal litter size.
The present invention is by carrying out many animals absorbing the research of α-ketoglutaric acid, and optimizing the animal that litter size significantly improves is: mouse, rat, pig, fox, rabbit and mink.Above-mentioned multiparous animal is after picked-up α-ketoglutaric acid, and other animals significantly enhance litter size more relatively.
Preferred daily dose and the weight ratio of the multiparous animals such as mouse, rat, pig, fox, rabbit and mink are 0.30g/kg.
Further, the present invention, respectively for above-mentioned animal, provides the method for the raising litter size be more preferably.
When described animal be mouse or rat time, at 2 ~ 12 monthly ages, every day is by oral way picked-up α-ketoglutaric acid 0.28 ~ 0.32g/kg;
As preferably, when the 2-3 monthly age, every daily ingestion 0.285g/kg, when the 3-4 monthly age, every daily ingestion 0.290g/kg, when the 4-5 monthly age, every daily ingestion 0.295g/kg, when the 5-6 monthly age, every daily ingestion 0.300g/kg, when the 6-7 monthly age, every daily ingestion 0.305g/kg, when the 7-8 monthly age, every daily ingestion 0.310g/kg, when the 8-9 monthly age, every daily ingestion 0.315g/kg, when the 9-10 monthly age, every daily ingestion 0.310g/kg, when the 10-11 monthly age, every daily ingestion 0.305g/kg, when the 11-12 monthly age, every daily ingestion 0.300g/kg.
When described animal is pig, at 14 ~ 84 monthly ages, every day is by oral way picked-up α-ketoglutaric acid 0.28 ~ 0.32g/kg;
As preferably, when the 14-21 monthly age, every daily ingestion 0.285g/kg, when the 21-28 monthly age, every daily ingestion 0.290g/kg, when the 28-35 monthly age, every daily ingestion 0.295g/kg, when the 35-42 monthly age, every daily ingestion 0.300g/kg, when the 42-49 monthly age, every daily ingestion 0.305g/kg, , during 49-56 monthly age, every daily ingestion 0.310g/kg, when the 56-63 monthly age, every daily ingestion 0.315g/kg, when the 63-70 monthly age, every daily ingestion 0.310g/kg, when the 70-77 monthly age, every daily ingestion 0.305g/kg, when the 77-84 monthly age, every daily ingestion 0.300g/kg.
When described animal be fox, rabbit or mink time, at 10 ~ 60 monthly ages, every day is by oral way picked-up α-ketoglutaric acid 0.28 ~ 0.32g/kg;
As preferably, when the 10-15 monthly age, every daily ingestion 0.285g/kg; When the 15-20 monthly age, every daily ingestion 0.290g/kg; When the 20-25 monthly age, every daily ingestion 0.295g/kg; When the 25-30 monthly age, every daily ingestion 0.300g/kg; When the 30-35 monthly age, every daily ingestion 0.305g/kg; When the 35-40 monthly age, every daily ingestion 0.310g/kg; When the 40-45 monthly age, every daily ingestion 0.315g/kg; When the 45-50 monthly age, every daily ingestion 0.310g/kg; When the 50-55 monthly age, every daily ingestion 0.305g/kg; When the 55-60 monthly age, every daily ingestion 0.300g/kg.
Further, the mode that described α-ketoglutaric acid is ingested is by adding in drinking water or animal feed by animal consumption.Every day is changed described feed or drinking water.
Beneficial effect of the present invention is:
Late Cambrian of the present invention enables multiparous animal oral suitable dose α-ketoglutaric acid improve the litter size of above the average age for marriage animal significantly, the reproductive efficiency of the above the average age for marriage animal of safe and effective raising.Make animal consumption α-ketoglutaric acid by the mode of adding α-ketoglutaric acid in the feed or drinking water of animal, can not only accomplish to add as required easily, change dosage in time, and can realize using on a large scale under mechanical feed feeds water system.
A-ketoglutaric acid used in the present invention can conveniently buied on the market, be applied to improve above the average age for marriage animal litter size aspect, considerable economic benefit and good social benefit can be brought: application the method can allow pig, fox, mink, Tu Deng plant and cultivation self-employed worker directly be benefited, improve nest litter size, improve the income of plant and cultivation self-employed worker; For the direct individual consumer of pork, rabbit meat, method described in this aspect also ensure that the edible safety of meat, ensure that the people are physically and mentally healthy, also promotes the development of aquaculture simultaneously.
Can numerous sow breeding stock 49,600 ten thousand by the whole nation in 2014, every sow annual is farrowed 1.5 nests, about 10, every nest.By improving average nest litter size 0.08 ~ 2.35, final live pig total amount of livestock for sale is by volume increase 5952 ~ 1,165,600,000, and direct economic benefit 297.6 ~ 5,828 hundred million yuan, overall economic benefit is considerable.
Accompanying drawing explanation
Fig. 1 takes the impact of different daily dose α-ketoglutaric acid on 6 monthly age mouse feelings phase breeding rates in experimental example 1 of the present invention;
Fig. 2 takes the impact of different daily dose α-ketoglutaric acid on 12 monthly age mouse feelings phase breeding rates in experimental example 1 of the present invention;
Fig. 3 takes the impact of different daily dose α-ketoglutaric acid on 6 monthly age mouse litter sizes in experimental example 1 of the present invention;
Fig. 4 takes the impact of different daily dose α-ketoglutaric acid on 12 monthly age mouse litter sizes in experimental example 1 of the present invention.
Embodiment
Below in conjunction with embodiment, the preferred embodiment of the present invention is described in detail.It will be appreciated that providing of following examples is only object in order to play explanation, being not used to limit scope of the present invention.Those skilled in the art, when not deviating from aim of the present invention and spirit, can carry out various amendment and replacement to the present invention.
The experimental technique used in following embodiment if no special instructions, is conventional method.
The reagent used in following examples and material are commercial goods.α-ketoglutaric acid (α-Ketoglutaricacid, the α-KG) available from Sigma (St.Louis, MO, USA) used.
Embodiment 1
The present embodiment relates to a kind of method being improved mouse litter size by oral α-ketoglutaric acid, and be specially in the feed or drinking-water α-ketoglutaric acid being added on mouse, the daily dose that mouse takes α-ketoglutaric acid is 0.05g/kg.Respectively when 6 monthly age of mouse, select the female mouse of spontaneous estrus, mate mating with public mouse, the next morning examines bolt, chooses and sees the female mouse of bolt, calculate feelings phase breeding rate, and add up litter size.
Embodiment 2 ~ 5
Compared with embodiment 1, distinctive points is only: in embodiment 2 ~ 5, and the daily dose taking α-ketoglutaric acid is respectively 0.00g/kg, 0.30g/kg, 0.78g/kg, 1.58g/kg.
Record display, can improve when the daily dose taking α-ketoglutaric acid is 0.05g/kg, 0.30g/kg 6 monthly ages and 12 the monthly age mouse feelings phase breeding rate and litter size.Daily dose 0.78g/kg, does not have effect to the mouse at 6 monthly ages, but can improve 12 the monthly age mouse feelings phase breeding rate and litter size.Daily dose 1.58g/kg, can significantly improve 6 the monthly age mouse litter size, but to 12 the monthly age mouse there is no effect.
Experimental example 1
1. laboratory animal
The female mouse of the Kunming white about 277 8 week ages is used to carry out above-described embodiment 1 ~ 5, the female mouse of Kunming white is purchased from Chinese military medicine academy of sciences Experimental Animal Center, female for Kunming white mouse is divided into 5 groups at random, often organize 50, female mouse can free choice feeding (Military Medical Science Institute's Experimental Animal Center produces full nutrition pellet) and drinking-water.
2. experiment condition is: illumination 12h/d, and indoor temperature is 20 ~ 22 DEG C.
3. experimentation
By the female mouse of experiment after the adaptability raising of a week, in feed or drinking-water, add α-ketoglutaric acid, the daily dose making female mouse take α-ketoglutaric acid is 0.05g/kg to 1.58g/kg, changes feed or drinking-water every day.When mouse 6 monthly age and 12 monthly age, will often organize female mouse and male mouse mates, morning inspection bolt.Continuous 4 days.Pick out female mouse that introitus has faint yellow solid (vaginal plug).A few days ago will see that the female mouse of bolt divides cage in the expected date of childbirth, raise separately, every day observes childbirth situation sooner or later.The nest litter size often organized added up in record.
4. experimental result
Data processing is carried out to the data result of above-mentioned record: adopt spass18.0 statistical software to carry out one-way analysis of variance (ANOVA) to data respectively, P<0.05 thinks that data have significant difference, and p<0.01 thinks that data difference heteropole is remarkable.The Multiple range test of average adopts DuncanShi to carry out, and result Means ± SEM represents.
4.1 different groups of impacts on mouse feelings phase breeding rate
Fig. 1 display except the feelings phase breeding rate of 0.78g/kg group abnormal on the low side except, feelings phase breeding rates of all the other each groups do not have too big-difference.
More each than all the other group of the feelings phase breeding rate that Fig. 2 shows 0.30g/kg group is all high, and all the other feelings phase breeding rates respectively organized do not have too big-difference.
4.2 different groups of impacts on mouse litter size
The impact of different daily dose α-ketoglutaric acid on 6 monthly age mouse litter sizes taken by table 1
| Group | α-ketoglutaric acid daily dose | Gravid mouse number (only) | Average nest litter size (only) |
| 8 week age young control | 0.00g/kg | 43 | 13.93±2.098 b |
| 6 monthly age control groups | 0.00g/kg | 50 | 12.52±2.288 a |
| 6 monthly age low dose group | 0.05g/kg | 42 | 12.6±2.307 ab |
| Low dose group in 6 monthly ages | 0.30g/kg | 49 | 14.2±2.227 bc |
| Dosage group in 6 monthly ages | 0.78g/kg | 43 | 12.51±2.453 a |
| 6 monthly age high dose group | 1.58g/kg | 50 | 13.45±2.189 ab |
Note: the different letter of shoulder mark represents difference and shows P<0.05, and shoulder mark represents difference containing same letter and do not show P>0.05.
From upper table 1, in 6 monthly age mouse, the average nest litter size of α-ketoglutaric acid daily dose 0.05g/Kg group, 0.30g/Kg group, 1.58g/Kg group is all significantly higher than control group, wherein in 6 monthly ages, the litter size of low dosage 0.30g/Kg group is more taller than control group in 8 week age, and 0.78g/Kg group has no significant effect nest litter size.
The impact of different daily dose α-ketoglutaric acid on 12 monthly age mouse litter sizes taken by table 2
Note: the different letter of shoulder mark represents difference and shows P<0.05, and shoulder mark represents difference containing same letter and do not show P>0.05.
From upper table 2, in 12 monthly age mouse, the average nest litter size of α-ketoglutaric acid daily dose 0.05g/Kg group, 0.30g/Kg group, 0.78g/Kg group is all significantly higher than control group, and 1.58g/Kg group has no significant effect nest litter size, but it is because old and feeble reason, all remarkable in control group in 8 week age.
In summary, daily dose of feeding is the average nest litter size that the α-ketoglutaric acid of 0.05g/Kg to 1.58g/Kg can improve mouse on certain level, especially when daily dose is 0.30g/Kg, nest litter size is improved the standard the most remarkable, and average every nest improves about about 2.In addition, the feelings phase breeding rate of daily dose 0.30g/Kg group mouse is also high compared with other experimental group.Therefore, method of the present invention is that the production practices improving multiparous animal litter size provide a kind of beneficial reference.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (8)
1. improve a method for above the average age for marriage animal reproduction power, it is characterized in that, make animal absorb α-ketoglutaric acid by oral way.
2. method according to claim 1, is characterized in that, the daily dose of animal consumption α-ketoglutaric acid is 0.05 ~ 1.58g/kg with the weight of animals ratio.
3. method according to claim 2, is characterized in that, the daily dose of animal consumption α-ketoglutaric acid is 0.30g/kg with the weight of animals ratio.
4. the method according to any one of claims 1 to 3, is characterized in that, described animal is mouse, rat, pig, fox, rabbit or mink.
5. method according to claim 4, is characterized in that, when described animal be mouse or rat time, at 2 ~ 12 monthly ages, every day is by oral way picked-up α-ketoglutaric acid 0.28 ~ 0.32g/kg;
As preferably, when the 2-3 monthly age, every daily ingestion 0.285g/kg, when the 3-4 monthly age, every daily ingestion 0.290g/kg, when the 4-5 monthly age, every daily ingestion 0.295g/kg, when the 5-6 monthly age, every daily ingestion 0.300g/kg, when the 6-7 monthly age, every daily ingestion 0.305g/kg, when the 7-8 monthly age, every daily ingestion 0.310g/kg, when the 8-9 monthly age, every daily ingestion 0.315g/kg, when the 9-10 monthly age, every daily ingestion 0.310g/kg, when the 10-11 monthly age, every daily ingestion 0.305g/kg, when the 11-12 monthly age, every daily ingestion 0.300g/kg.
6. method according to claim 4, is characterized in that, when described animal is pig, at 14 ~ 84 monthly ages, every day is by oral way picked-up α-ketoglutaric acid 0.28 ~ 0.32g/kg;
As preferably, when the 14-21 monthly age, every daily ingestion 0.285g/kg, when the 21-28 monthly age, every daily ingestion 0.290g/kg, when the 28-35 monthly age, every daily ingestion 0.295g/kg, when the 35-42 monthly age, every daily ingestion 0.300g/kg, when the 42-49 monthly age, every daily ingestion 0.305g/kg, , during 49-56 monthly age, every daily ingestion 0.310g/kg, when the 56-63 monthly age, every daily ingestion 0.315g/kg, when the 63-70 monthly age, every daily ingestion 0.310g/kg, when the 70-77 monthly age, every daily ingestion 0.305g/kg, when the 77-84 monthly age, every daily ingestion 0.300g/kg.
7. method according to claim 4, is characterized in that, when described animal be fox, rabbit or mink time, at 10 ~ 60 monthly ages, every day is by oral way picked-up α-ketoglutaric acid 0.28 ~ 0.32g/kg;
As preferably, when the 10-15 monthly age, every daily ingestion 0.285g/kg; When the 15-20 monthly age, every daily ingestion 0.290g/kg; When the 20-25 monthly age, every daily ingestion 0.295g/kg; When the 25-30 monthly age, every daily ingestion 0.300g/kg; When the 30-35 monthly age, every daily ingestion 0.305g/kg; When the 35-40 monthly age, every daily ingestion 0.310g/kg; When the 40-45 monthly age, every daily ingestion 0.315g/kg; When the 45-50 monthly age, every daily ingestion 0.310g/kg; When the 50-55 monthly age, every daily ingestion 0.305g/kg; When the 55-60 monthly age, every daily ingestion 0.300g/kg.
8. the method according to claims 1 to 3 or 5 ~ 7 any one, is characterized in that, described α-ketoglutaric acid is absorbed by multiparous animal by adding in animal feed or drinking water.
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