CN105467080B - Sitodiplosis mosellana number detection method - Google Patents
Sitodiplosis mosellana number detection method Download PDFInfo
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- CN105467080B CN105467080B CN201510827268.7A CN201510827268A CN105467080B CN 105467080 B CN105467080 B CN 105467080B CN 201510827268 A CN201510827268 A CN 201510827268A CN 105467080 B CN105467080 B CN 105467080B
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- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 241000068648 Sitodiplosis mosellana Species 0.000 title abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 56
- 241000209140 Triticum Species 0.000 claims abstract description 38
- 235000021307 Triticum Nutrition 0.000 claims abstract description 38
- 238000000034 method Methods 0.000 claims abstract description 38
- 238000007654 immersion Methods 0.000 claims abstract description 17
- 238000005406 washing Methods 0.000 claims abstract description 8
- 241001663467 Contarinia tritici Species 0.000 claims description 7
- 238000007689 inspection Methods 0.000 claims description 7
- 238000005070 sampling Methods 0.000 claims description 6
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 3
- 241000256135 Chironomus thummi Species 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 8
- 230000000007 visual effect Effects 0.000 abstract 1
- 235000013339 cereals Nutrition 0.000 description 7
- 241000238631 Hexapoda Species 0.000 description 5
- 239000010903 husk Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000012353 t test Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000333702 Thyridopteryx ephemeraeformis Species 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004890 malting Methods 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
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- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
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- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a sitodiplosis mosellana number detection method including the steps of field spike picking, water immersion detection and spike washing detection, wherein the water immersion detection comprises the steps of placing a wheat spike having immersed in water into a plastic bag to make sitodiplosis mosellana automatically pop up, recording the number of the sitodiplosis mosellana in the water, and repeating the steps for multiple times; the spike washing detection comprises the steps of rubbing the wheat spike after water immersion detection in water, then recording the number of the sitodiplosis mosellana in the water, repeating the steps for multiple times to obtain the final total number of the sitodiplosis mosellana. The method not only greatly improves the sitodiplosis mosellana detection speed, but also increases the accuracy of survey data, has the advantages of visual observation, easy identification, convenience for counting, and fastness and accuracy, and achieves the purposes of improving economic benefits, ecological benefits and social benefits.
Description
Technical field
The present invention relates to Wheat Diseases And Insect Pests detection method, and in particular to a kind of wheat midge quantity detection method.
Background technology
Wheat midge is worldwide primary pest, even more the important pests of China's wheat.Hidden in grain husk with larva
The wheat juice being in the milk is sucked in shell, blighted grain, ghost is caused.Wheat midge is caused harm floral organ, seed and/or wheat with larva
Grain, is a kind of crushing insect.Last century early fifties are rampant to be occurred, long-term 1～2 one-tenth of underproduction wheat, and outbreak year part can
Up to 4～5 one-tenth, serious up to 8～9 one-tenth, after once controlled.Last century enters the eighties, gos up comprehensively, anti-because observing and predicting
Reason is controlled, heavy losses are caused repeatedly.National occurring area is more than 2,670,000 hectares within 1994.Henan Insect appearance area reaches within 2005
1530000 hectares, it is related to 104 980, counties small towns, average hundred ear worms amount 239.3, hundred ear worm amount of highest 35000, local wheat
Field has no harvest.Because suction pulp worm spawning has stronger selectivity to kind, breeding time, in addition polypide is tiny, different year, different fields
Block occurs usually have very big uncertainty, quick, accurately observe and predict always suction pulp worm and observe and predict by the restriction of these subjective and objective factors
Preventing and treating a great problem.
At present, widely used stripping fringe and soil inspection method is washed in a pan, exists time-consuming, identification is difficult, high labor intensive,
The defects such as technical conditions height are sought, sampling more than 10 can only be limited in agricultural industry criteria in 2002 observes and predicts specification, far from
Can meet and observe and predict preventing and treating needs, so that Jing leakage often occurs and controls and agricultural chemicals abuse in actually preventing and treating, cause environmental pollution and food
Safety problem.
The content of the invention
The technical problem to be solved is how rapidly and accurately to detect the quantity contained by wheat midge, from
And take necessary prophylactico-therapeutic measures, it is ensured that the yield and quality of wheat.
To solve above-mentioned technical problem, the technical solution used in the present invention is：A kind of wheat midge quantity detection side
Method, including following methods step：
(1). adopt fringe in field：Deviate from the larva occurance peak before the wheat head to larva within 10 days after wheat flowering, by wheatland single pair of
5 points of samplings of linea angulata, per optional 10～30 fringe is put in paper bag and is detected；
(2). immersion detection：The wheat head to be checked is put in polybag, clear water is poured into, is allowed to all be dipped in clear water at least
10s, then takes out the wheat head, in pouring water in bag into white or blue basin, the wheat head is reentered in polybag, after lashing
Mark is carried out, is hung on cross bar, allow suction pulp worm to eject naturally；Clear water in basin is poured out slowly, only stays little water to be easy to observation meter
Number, keeps suction pulp worm larva quantity in mind；After 1～7 day, the pendulum that adds water washes the suction pulp worm outside fringe and on polybag inwall, after pendulum is washed
Still it is put in bag according to preceding method, continues to allow suction pulp worm in fringe to eject；To pour in washbowl containing suction pulp worm water every time, use
Same method is stated, suction pulp worm quantity in water is checked；When population number is larger, by the suction pulp worm with a small amount of water, lattice has been poured into
White flat bottom disk in, so as to accurate counting；
(3). wash in a pan fringe detection：By the wheat head Jing after ejecting suction pulp worm naturally, rub in the white or blue basin being filled with water, under
The mesh screen in a 1mm apertures is put by side, after rubbing several times in water, mesh screen is above carried, then slowly submerged, 2～3 times repeatedly, so
Water above is slowly poured out afterwards, is left bottom suction pulp worm and is counted；After counting in aforementioned manners, carry out the 2nd, the 3rd time and eluriate inspection；Most
Total midge borer population is counted afterwards.
The present invention is soaked using the wheat midge quantity detection method designed by above-mentioned technical proposal, test with clear water
Afterwards, fringe is washed in a pan in water, tradition stripping fringe method is substituted, inspection speed is not only greatly improved, while survey data accuracy is improved,
Better effects are obtained in practical application.The present invention has that observation is directly perceived, identification easily, count easily and fast accurate advantage.Especially
Adapt to that current basic unit of China professional science and technology is undermanned, the generally not high scientific and technological present situation of technical quality.Can be used for aborning fast
Speed, accurately determination controlling object field, make in advance early warning, instruct pest-resistant cultivar to utilize in advance, so as to reduce Pesticide use, reduce
Environmental pollution, saves preventing and treating recruitment, and it is not enough to alleviate rural labour, reach increase economic efficiency, ecological benefits, social benefit mesh
's.
Specific embodiment
Wheat midge quantity detection method of the present invention, including following methods step：
(1). fringe is adopted in field, in larva occurance peak (before 10 days after wheat flowering deviate from the wheat head to larva), in insect density
Larger field, by the 5 points of samplings of single pair of linea angulata, per optional 10 fringe, in being put into paper bag, after taking back the indoor immersion for systematic observation
Suction pulp worm ejects situation naturally, and remaining worm amount in fringe inspection fringe is washed in a pan after 7d；
(2). immersion detection, the fringe of the wheat head to be checked 50 is put into into long 40cm, in the polybag of wide 30cm, 5 liters of clear water is poured into, make
It is to be all dipped in clear water at least 10s, the wheat head is then taken out, pour water in bag into diameter 30cm, the white or blueness of high 20cm
In basin, the wheat head is reentered in polybag, mark is carried out after lashing, hung with cross bar, allow suction pulp worm to eject naturally；Will
Clear water is poured out slowly in basin, only stays little water to be easy to observation to count, and keeps suction pulp worm larva quantity in mind.The pendulum that added water after 1～7 day is washed
Suction pulp worm on fringe outside and polybag inwall, pendulum is still put in bag after washing according to front method, continues to allow suction pulp worm in fringe to eject；Often
It is secondary to pour in washbowl containing suction pulp worm water, above-mentioned same method is used, suction pulp worm quantity in water is checked, when population number is larger,
By the suction pulp worm with a small amount of water, the long 20cm of lattice is poured into, in the white flat bottom disk of wide 30cm, high 4cm, accurately to count
Number；
(3). fringe detection is washed in a pan, the wheat head Jing after ejecting suction pulp worm naturally is rubbed in the white or blue basin being filled with water, under
A bore 25cm puts in side, and deep 10cm, the mesh screen in 1mm apertures after rubbing several times in water, mesh screen is above carried, and then slowly sinks to water
In, 2～3 times repeatedly, water above is then slowly poured out, leave bottom suction pulp worm and count；After counting in aforementioned manners, carry out the 2nd,
Eluriate for 3rd time and check.
Test application
In larva occurance peak, 10 pieces of different type field is chosen, every piece of field single pair of 5 points of samplings of linea angulata, per 20 fringes are divided into
2 parts, 2 paper bags are respectively charged into, with tradition stripping fringe method and method of the present invention population number is checked.
With conventional method, indoors directly stripping is looked into, and total grain number, aggrieved grain number, borer population are investigated thoroughly by fringe, by grain, if having children in bag
Worm, counts in the lump；Look into simultaneously in stripping, one newspaper of lower section will shell the worm after fringe and broken fringe is all accessed thereon, then with above-mentioned
Naughty fringe detection method is verified, and is deducted directly stripping with total borer population and is looked into borer population, obtains leakage borer population, calculates under-enumeration ratio.
As a result with analysis
Using the method for the present invention, wheat midge climbs out of successively clever shell after immersion, i.e., springs into bag wall and bottom,
There is ejection peak (table 1), accounts for total borer population 49.5% in bottom moisture abundance environment, seldom activity, the same day, ejects slow afterwards
Slowly, 4d rear portions molecule grain starts germination, ejects to 7d and has reached 85%.Wheat rudiment is ejected on suction pulp worm affects little, but
Part bud point fractures and is sunken to the bottom, slightly affects on washing in a pan fringe observation, therefore, eject naturally and terminated at once with small part malting
It is advisable.
The bowssening suction pulp worm of table 1 is ejected and naughty fringe method result of the test naturally
During naughty fringe during rubbing, suction pulp worm separate with clever shell it is latter as 3～5s can naturally be down to basin bottom, on pouring out
During portion's excessive moisture, here it is apparent that position of the suction pulp worm in water, pouring speed is adjusted, can be by the suction pulp worm of bottom
Separate with top clear water, a time elutriation of Jing, accumulative to detect 98.2% suction pulp worm, Jing can reach wheat for tri- times with clever shell whole point
From naughty net whole larvas.Process is rubbed in elutriation, tattered polypide is not found, polypide is obtained and inspection after 5d is placed in water, it is raw
Deposit good.
The present invention and conventional stripping fringe method comparison and analysis
Conventional stripping fringe method stripping obtains worm amount (x1) average 236.3, by carrying out recovery inspection with naughty fringe method to omitting borer population
Look into, obtain shelling carries out washing in a pan fringe recovery worm amount (x after fringe2), Jing tests carry out again washing in a pan fringe recovery after stripping fringe, and various kinds is more normal than original
Rule stripping ear worm amount has increase in various degree, and increase ratio is 0.7～52.9% (table 2), averagely reaches 26.1%, two groups of data
Jing t test, t12=2.3637, difference reaches the level of signifiance, illustrate tradition stripping fringe method acquisition result and actual conditions have it is larger go out
Enter；Another group of sample, without stripping fringe, directly washes in a pan fringe method and obtains worm amount (x with immersion3), than first group of sample tradition stripping fringe method
Check total worm amount (x1) high 30.6%, Jing significance test of differences, t13=2.3176, both differences reach the level of signifiance, explanation
Fringe method stripping fringe method more direct than first group of sample is washed in a pan in the immersion of second group of sample, and to strip out borer population more, is because method is improved；
Fringe method is washed in a pan with immersion for second group obtain worm amount (x3)Borer population (x is reclaimed with fringe is washed in a pan after first group of stripping fringe2) compare, then it is closer to, put down
Mean only differs 2.0%, and two groups of data Jing t test, t23=0.7996, the level of signifiance is not reached, illustrate that difference is due to sampling
Caused by accidentalia, method and actual conditions closer to.
The conventional stripping fringe of table 2 washes in a pan fringe method and checks comparative result with immersion
The fail-safe analysis of the present invention
Further analysis, due to suction pulp worm polypide itself it is less, while and due to there is complexity between wheat and wheat husk
Structure, conventional method is directly shelled when looking into, and is often difficult to find some hiding suction pulp worms from an angular observation, even if completely sudden and violent
Two suction pulp worms of dew, side by side, in the case of, because polypide is less, visually-perceptible is usually mistaken for a polypide, no
It is often very big with investigation personal investigation error, directly affect the accuracy and reliability of survey data.Immersion wash in a pan fringe method not only after
Hold tradition stripping fringe method with strong points, representative good feature, using suction pulp worm and the higher feature of water proportion, by sieve and wheat husk,
Wheat debris natural separation, observes, good dispersion in the water of white disk, without the interference of the debris such as wheat husk, is easy to operation
Control, the characteristics of subjective and objective factor affects little, thus with higher accuracy and reliability.
The operating efficiency analysis of the present invention
3～5m when tradition stripping fringe method stripping fringe typically often shells 1 fringe palpus, if completing 150～250m during the fringe of sample 50 palpus,
By standard sample, the used time often exceeds naughty local method.In practical operation, in order to improve speed, much take and down-sample quantity
Method, it is representative that this certainly will reduce survey data.Fringe method is washed in a pan using immersion to check, typically complete 15～25m during a sample palpus,
Can improve work efficiency 80%～90% than tradition stripping fringe method, and operate the used time to increase with sample size affects little.
The application effect of the present invention
Achievement Jing 2013～2015 years can accurate, convenient, fast determination wheat midge generation feelings in local application
Condition, reduces insect survey heavy labor.Except professional can be with the convenient use technology, to plant protection cooperative society, agricultural
Planting industry cooperative society is convenient to use.For instructing preventing and treating, 25.6 ten thousand mu are prevented and treated, 2238.79 ten thousand yuan of direct economic benefit；
Unnecessary 15.3 ten thousand mu of preventing and treating is reduced, 31 tons of medication is reduced, to reducing pollution of area source, safeguarding agricultural product quality and safety, prevent ring
Border destruction, promotion restoration of the ecosystem have played important function.
The problem that should be noted that in present invention application
Bowssening is difficult to be automatically popped up completely in a short time, must can reach wheat Jing after immersion with naughty fringe method connected applications
Shell is soft, and saving is rubbed the time, improves seed and wheat husk separating effect, and to carry out washing in a pan fringe operation more suitable for 1～3d after immersion, immersion and
Naughty fringe operation once needs water 10L or so per sample, and water can be recycled, the interchangeable water of 2～3 samples of process, keeps water more clear
It is clear, in order to operate observation.
Claims (1)
1. a kind of wheat midge quantity detection method, is characterized in that including following methods step：
(1). adopt fringe in field：Deviate from the larva occurance peak before the wheat head to larva within 10 days after wheat flowering, by wheatland single pair of linea angulata
5 points of samplings, per optional 10～30 fringe is put into be detected in paper bag；
(2). immersion detection：The wheat head to be checked is put in polybag, clear water is poured into, is allowed to all be dipped in clear water at least 10s, so
After take out the wheat head, in pouring water in bag into white or blue basin, the wheat head is reentered in polybag, mark is carried out after lashing
Note, is hung on cross bar, allows suction pulp worm to eject naturally；Clear water in basin is poured out slowly, only stays little water to be easy to observation to count, kept in mind
Suction pulp worm larva quantity；After 1～7 day, the pendulum that adds water washes the suction pulp worm outside fringe and on polybag inwall, and pendulum takes out wheat after washing
Fringe, in pouring water in bag into white or blue basin, the wheat head is reentered in polybag, and mark is carried out after lashing, and is hung on
On cross bar, continue to allow suction pulp worm in fringe to eject；To pour in washbowl containing suction pulp worm water every time, with above-mentioned same method water will be checked
Middle suction pulp worm quantity；When population number is larger, by the suction pulp worm with a small amount of water, in pouring the white flat bottom disk of lattice into, with
Just accurate counting；
(3). wash in a pan fringe detection：By the wheat head Jing after ejecting suction pulp worm naturally, rub in the white or blue basin being filled with water, lower section is put
The mesh screen in one 1mm apertures, after rubbing several times in water, mesh screen is above carried, then slowly submerged, 2～3 times repeatedly, Ran Houhuan
It is slow to pour out water above, leave bottom suction pulp worm and count；Carry out the 2nd, the 3rd time after counting in aforementioned manners and eluriate inspection；Finally unite
The total midge borer population of meter.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510827268.7A CN105467080B (en) | 2015-11-25 | 2015-11-25 | Sitodiplosis mosellana number detection method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510827268.7A CN105467080B (en) | 2015-11-25 | 2015-11-25 | Sitodiplosis mosellana number detection method |
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| CN105467080A CN105467080A (en) | 2016-04-06 |
| CN105467080B true CN105467080B (en) | 2017-05-03 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN202907550U (en) * | 2012-09-11 | 2013-05-01 | 鹤壁佳多科工贸有限责任公司 | Intelligent soil excavation device for wheat sitodiplosis mosellana |
| CN204232011U (en) * | 2014-11-13 | 2015-04-01 | 河南省农业科学院植物保护研究所 | Wheat midge washes in a pan soil, rinsing, sorting equipment |
| CN104429736B (en) * | 2014-11-13 | 2016-07-27 | 河南省农业科学院植物保护研究所 | Wheat midge washes in a pan soil, rinsing, sorting equipment and using method thereof |
| CN204241263U (en) * | 2014-12-18 | 2015-04-01 | 河南省农业科学院植物保护研究所 | Wheat midge soil sample barrel |
| CN104621076B (en) * | 2015-01-28 | 2017-03-15 | 河南省农业科学院植物保护研究所 | Wheat midge trap |
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