CN105467080A - Sitodiplosis mosellana number detection method - Google Patents
Sitodiplosis mosellana number detection method Download PDFInfo
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- CN105467080A CN105467080A CN201510827268.7A CN201510827268A CN105467080A CN 105467080 A CN105467080 A CN 105467080A CN 201510827268 A CN201510827268 A CN 201510827268A CN 105467080 A CN105467080 A CN 105467080A
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- 238000001514 detection method Methods 0.000 title claims abstract description 16
- 241000068648 Sitodiplosis mosellana Species 0.000 title abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 54
- 238000000034 method Methods 0.000 claims abstract description 46
- 241000209140 Triticum Species 0.000 claims abstract description 35
- 235000021307 Triticum Nutrition 0.000 claims abstract description 35
- 238000007654 immersion Methods 0.000 claims abstract description 17
- 238000005406 washing Methods 0.000 claims abstract description 5
- 241000256135 Chironomus thummi Species 0.000 claims description 42
- 241001663467 Contarinia tritici Species 0.000 claims description 7
- 238000007689 inspection Methods 0.000 claims description 7
- 238000005070 sampling Methods 0.000 claims description 7
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 3
- 230000008901 benefit Effects 0.000 abstract description 8
- 230000000007 visual effect Effects 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 5
- 239000010903 husk Substances 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004890 malting Methods 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a sitodiplosis mosellana number detection method including the steps of field spike picking, water immersion detection and spike washing detection, wherein the water immersion detection comprises the steps of placing a wheat spike having immersed in water into a plastic bag to make sitodiplosis mosellana automatically pop up, recording the number of the sitodiplosis mosellana in the water, and repeating the steps for multiple times; the spike washing detection comprises the steps of rubbing the wheat spike after water immersion detection in water, then recording the number of the sitodiplosis mosellana in the water, repeating the steps for multiple times to obtain the final total number of the sitodiplosis mosellana. The method not only greatly improves the sitodiplosis mosellana detection speed, but also increases the accuracy of survey data, has the advantages of visual observation, easy identification, convenience for counting, and fastness and accuracy, and achieves the purposes of improving economic benefits, ecological benefits and social benefits.
Description
Technical field
The present invention relates to Wheat Diseases And Insect Pests detection method, be specifically related to a kind of wheat midge quantity detection method.
Background technology
Wheat midge is worldwide primary pest, especially the important pests of China's wheat.To hide with larva the wheat juice sucked in clever shell and be in the milk, cause blighted grain, ghost.Wheat midge with larva cause harm floral organ, seed and or wheat, be a kind of crushing insect.Last century, early fifties wildness occurred, long-term underproduction wheat 1 ~ 2 one-tenth, and outbreak year part can reach 4 ~ 5 one-tenth, serious in 8 ~ 9 one-tenth, after once controlled.Last century enters the eighties, gos up comprehensively, preventing and treating reason, causing heavy losses repeatedly because observing and predicting.Within 1994, national occurring area is more than 2,670,000 hectares.Within 2005, Henan Insect appearance area reaches 1,530,000 hectares, relates to small towns, 980,104 counties, average hundred ear worm amount 239.3, the highest hundred ear worm amount 35000, local wheatland total crop failure.Because midge worm lays eggs to kind, breeding time having stronger selectivity, in addition polypide is tiny, different year, different field occur usually there is very large uncertainty, and restrict by these subjective and objective factors, fast, accurately observe and predict is that midge worm observes and predicts control a great problem always.
At present, the stripping fringe of extensive employing and naughty native inspection method, exist time-consuming, not easily, labour intensity is large in identification, requires the defects such as technical conditions are high, observe and predict in specification at agricultural industry criteria in 2002 and can only limit sampling more than 10, can not meet far away and observe and predict control needs, so that in reality control, often occur that leakage is controlled and agricultural chemicals abuse, cause environmental pollution and food-safety problem.
Summary of the invention
Technical matters to be solved by this invention how to detect the quantity contained by wheat midge rapidly and accurately, thus take necessary prophylactico-therapeutic measures, guarantees the seed output and quality of wheat.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of wheat midge quantity detection method, comprises following methods step:
(1). fringe is adopted in field: the larva occurance peak before deviating from the wheat head to larva in 10 days after wheat flowering, and by wheatland list diagonal line 5 sampling, often optional 10 ~ 30 fringes, put into paper bag and be detected;
(2). immersion detects: the wheat head to be checked is put into polybag, pour into clear water, to make it all to be dipped in clear water at least 10s, then the wheat head is taken out, water in bag is poured in white or blue basin, the wheat head is reentered in polybag, after lashing, carries out mark, be hung on cross bar, allow midge worm naturally eject; Clear water in basin is poured out slowly, only stays little water to be convenient to observe counting, keep midge worm larva quantity in mind; After 1 ~ 7 day, the pendulum that adds water washes the midge worm on the outside and polybag inwall of fringe, and pendulum still puts into bag according to preceding method after washing, and continues to allow midge worm in fringe eject; To pour in washbowl containing midge worm water at every turn, use above-mentioned same method, check midge borer population amount in water; When population number is larger, by the midge worm with a small amount of water, pour in the white flat bottom dish of lattice, so that accurate counting;
(3). wash in a pan fringe and detect: by the wheat head after ejection midge worm naturally, rub in the white be filled with water or blue basin, the mesh screen in a 1mm aperture is put in below, rub in water several times, carry on mesh screen, then slowly submerged, 2 ~ 3 times repeatedly, then slowly pour out water above, leave bottom midge worm counting; Count rear said method, carry out the 2nd, the 3rd time and eluriate inspection; Finally add up total midge borer population.
The present invention adopts the wheat midge quantity detection method designed by technique scheme, and test washes in a pan fringe after soaking with clear water in water, substitute tradition stripping fringe method, not only greatly improve inspection speed, improve enquiry data accuracy simultaneously, obtain better effects in actual applications.The present invention have observe directly perceived, identification easily, counting accurate advantage easily and fast.Especially basic unit of current China professional science and technology is adapted to undermanned, the scientific and technological present situation that technical quality is generally not high.Can be used for aborning fast, accurately determining controlling object field, make early warning in advance, instruct pest-resistant cultivar to utilize in advance, thus minimizing Pesticide use, reduce environmental pollution, save control recruitment, alleviate rural labour not enough, reach increase economic efficiency, ecological benefits, social benefit object.
Embodiment
Wheat midge quantity detection method of the present invention, comprises following methods step:
(1). fringe is adopted in field, in larva occurance peak (before within after wheat flowering 10 days, deviating from the wheat head to larva), in the larger field of insect density, by single diagonal line 5 sampling, often optional 10 fringes, put into paper bag, take back indoor for midge worm nature ejection situation after systematic observation immersion, and naughty fringe checks remaining worm amount in fringe after 7d;
(2). immersion detects, the wheat head 50 fringe to be checked is put into long 40cm, in the polybag of wide 30cm, pours into 5 liters, clear water, to make it all to be dipped in clear water at least 10s, then take out the wheat head, pour water in bag into diameter 30cm, in the white of high 20cm or blue basin, the wheat head is reentered in polybag, carry out mark after lashing, hang with on cross bar, allow midge worm naturally eject; Clear water in basin is poured out slowly, only stays little water to be convenient to observe counting, keep midge worm larva quantity in mind.The pendulum that added water after 1 ~ 7 day washes the midge worm on the outside and polybag inwall of fringe, and pendulum still puts into bag according to front method after washing, and continues to allow midge worm in fringe eject; To pour in washbowl containing midge worm water at every turn, use above-mentioned same method, and check midge borer population amount in water, when population number is larger, by the midge worm with a small amount of water, pour the long 20cm of lattice into, wide 30cm, in the white flat bottom dish of high 4cm, so that accurate counting;
(3). wash in a pan fringe and detect, by through naturally ejecting the wheat head after midge worm, rub in the white be filled with water or blue basin, a bore 25cm is put in below, dark 10cm, the mesh screen in 1mm aperture, rubs in water several times, carries on mesh screen, then slowly submerged, 2 ~ 3 times repeatedly, then slowly pour out water above, leave bottom midge worm counting; Count rear said method, carry out the 2nd, the 3rd time and eluriate inspection.
Test application
At larva occurance peak, choose 10 pieces, dissimilar field, single diagonal line 5 sampling in every block field, often 20 fringes, are divided into 2 parts, are respectively charged into 2 paper bags, check population number by tradition stripping fringe method and method of the present invention.
Directly to shell in indoor by classic method and look into, by fringe, investigate thoroughly total grain number by grain, be injured a number, borer population, if having larva in bag, count in the lump; Look into simultaneously in stripping, the worm after stripping fringe and broken fringe are all accessed on it by below one newspaper, then verify by above-mentioned naughty fringe detection method, deduct directly stripping and look into borer population, obtain leakage borer population, calculate under-enumeration ratio with total borer population.
Results and analysis
Adopt method of the present invention, wheat midge is after immersion, namely climb out of clever shell successively, spring into bag wall and bottom, at the sufficient environment of bottom moisture, little activity, namely occurred the same day ejecting peak (table 1), accounted for total borer population 49.5%, ejected slowly afterwards, 4d rear section seed starts to germinate, and ejects add up to reach 85% to 7d.It is little that wheat rudiment ejects impact to midge worm, but part bud point fractures and is sunken to the bottom, and slightly affect naughty fringe observation, therefore, ejection terminates to be advisable with small part malting at once naturally.
Table 1 bowssening midge worm ejects and naughty fringe method test findings naturally
During naughty fringe in the process of rubbing, midge worm is separated rear general 3 ~ 5s and can is naturally down at the bottom of basin with clever shell, pouring out in the excessive moisture process of top, can know and see the position of midge worm in water, regulate pouring speed, the midge worm of bottom can be separated with top clear water, eluriate through one time, add up to detect 98.2% midge worm, wheat can be reached through three times and all be separated with clever shell, wash in a pan clean all larvas.Rub process in elutriation, do not find tattered polypide, obtain after polypide places 5d in water and check, existence is good.
The present invention shells fringe method comparison and analysis with conventional
The stripping of conventional stripping fringe method obtains worm amount (x
1) average 236.3, by carrying out recovery inspection to the naughty fringe method of omission borer population, carrying out naughty fringe after obtaining stripping fringe and reclaiming worm amount (x
2), through test, by carrying out naughty fringe recovery again after stripping fringe, various kinds all has than former routine stripping ear worm amount to be increased in various degree, and increase ratio is 0.7 ~ 52.9% (table 2), on average reaches 26.1%, and two groups of data are test through t, t
12=2.3637, difference reaches the level of signifiance, illustrates that tradition stripping fringe method obtains result and actual conditions have greater difference; Another organizes sample, without stripping fringe, directly washes in a pan fringe method with immersion and obtains worm amount (x
3), check out total worm amount (x than first group of sample tradition stripping fringe method
1) high by 30.6%, through significance test of difference, t
13=2.3176, both reach the level of signifiance at difference, and illustrating that the immersion of second group of sample is washed in a pan fringe method and directly shelled fringe method than first group of sample to strip out borer population more, is caused by method improvement; Wash in a pan fringe method with immersion for second group and obtain worm amount (x
3)wash in a pan fringe after shelling fringe with first group and reclaim borer population (x
2) compare, then comparatively close, average only differs 2.0%, and two groups of data are test through t, t
23=0.7996, do not reach the level of signifiance, illustrate that difference is that method and actual conditions are more close caused by sampling accidentalia.
Table 2 routine stripping fringe is washed in a pan fringe method with immersion and is checked comparative result
Fail-safe analysis of the present invention
Further analysis, because the polypide of midge worm own is less, simultaneously again owing to there is complicated structure between wheat and wheat husk, when classic method is directly shelled and looked into, be often difficult to the midge worm finding that some are hidden from an angular observation, even if expose completely two midge worms, when close side by side, because polypide is less, visually-perceptible is usually mistaken for a polypide, different investigator's survey error is often very large, directly affects accuracy and the reliability of enquiry data.Fringe method is washed in a pan in immersion, and not only inheriting tradition stripping fringe method is with strong points, representative good feature, utilize midge worm and the higher feature of water proportion, by sieve and wheat husk, wheat foreign material natural separation, observe in the water of white disk, good dispersion, there is no the foreign material interference such as wheat husk, have operation to be easy to control, subjective and objective factor affects little feature, thus has higher accuracy and reliability.
Work efficiency analysis of the present invention
Tradition stripping fringe method stripping fringe generally often shell 1 fringe must time 3 ~ 5m, if complete sample 50 fringe must time 150 ~ 250m, by standard sample, the used time often exceeds naughty local method.In practical operation, in order to improve speed, much take the method reducing quantity of sampling quantity, this certainly will reduce enquiry data representativeness.Adopt immersion to wash in a pan the inspection of fringe method, generally complete sample must time 15 ~ 25m, work efficiency 80% ~ 90% can be improved than tradition stripping fringe method, and operating the used time, to increase impact with sample size little.
Effect of the present invention
This achievement is through 2013 ~ 2015 years in locality application, and a situation arises accurate, convenient, fastly can to determine wheat midge, reduces insect survey heavy labor.Except professional can this technology easy to use, to plant protection cooperative society, the also very convenient use of agricultural planting industry cooperative society.Be used to guide control, prevent and treat 25.6 ten thousand mu, direct economic benefit 2238.79 ten thousand yuan; Reduce unnecessary control 15.3 ten thousand mu, reduce medication 31 tons, to reducing pollution of area source, safeguarding agricultural product quality and safety, prevent environmental disruption, promoting that restoration of the ecosystem has played vital role.
Attention question is answered in the present invention's application
Bowssening is difficult to eject completely voluntarily in a short time, must with naughty fringe method connected applications, wheat husk softness can be reached after immersion, save and rub the time, improve seed and wheat husk separating effect, it is more suitable that the rear 1 ~ 3d of immersion carries out naughty fringe operation, immersion and the every sample of naughty fringe operation once need water about 10L, and water can be recycled, and processes 2 ~ 3 interchangeable water of sample, keep water comparatively limpid, so that operation observation.
Claims (1)
1. a wheat midge quantity detection method, is characterized in that comprising following methods step:
(1). fringe is adopted in field: the larva occurance peak before deviating from the wheat head to larva in 10 days after wheat flowering, and by wheatland list diagonal line 5 sampling, often optional 10 ~ 30 fringes, put into paper bag and be detected;
(2). immersion detects: the wheat head to be checked is put into polybag, pour into clear water, to make it all to be dipped in clear water at least 10s, then the wheat head is taken out, water in bag is poured in white or blue basin, the wheat head is reentered in polybag, after lashing, carries out mark, be hung on cross bar, allow midge worm naturally eject; Clear water in basin is poured out slowly, only stays little water to be convenient to observe counting, keep midge worm larva quantity in mind; After 1 ~ 7 day, the pendulum that adds water washes the midge worm on the outside and polybag inwall of fringe, and pendulum still puts into bag according to preceding method after washing, and continues to allow midge worm in fringe eject; To pour in washbowl containing midge worm water at every turn, use above-mentioned same method, check midge borer population amount in water; When population number is larger, by the midge worm with a small amount of water, pour in the white flat bottom dish of lattice, so that accurate counting;
(3). wash in a pan fringe and detect: by the wheat head after ejection midge worm naturally, rub in the white be filled with water or blue basin, the mesh screen in a 1mm aperture is put in below, rub in water several times, carry on mesh screen, then slowly submerged, 2 ~ 3 times repeatedly, then slowly pour out water above, leave bottom midge worm counting; Count rear said method, carry out the 2nd, the 3rd time and eluriate inspection; Finally add up total midge borer population.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510827268.7A CN105467080B (en) | 2015-11-25 | 2015-11-25 | Sitodiplosis mosellana number detection method |
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|---|---|---|---|
| CN201510827268.7A CN105467080B (en) | 2015-11-25 | 2015-11-25 | Sitodiplosis mosellana number detection method |
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| CN105467080A true CN105467080A (en) | 2016-04-06 |
| CN105467080B CN105467080B (en) | 2017-05-03 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202907550U (en) * | 2012-09-11 | 2013-05-01 | 鹤壁佳多科工贸有限责任公司 | Intelligent soil excavation device for wheat sitodiplosis mosellana |
| CN104429736A (en) * | 2014-11-13 | 2015-03-25 | 河南省农业科学院植物保护研究所 | Wheat blossom midge soil removal, rinsing and sorting device and use method thereof |
| CN204241263U (en) * | 2014-12-18 | 2015-04-01 | 河南省农业科学院植物保护研究所 | Wheat midge soil sample barrel |
| CN204232011U (en) * | 2014-11-13 | 2015-04-01 | 河南省农业科学院植物保护研究所 | Wheat midge washes in a pan soil, rinsing, sorting equipment |
| CN104621076A (en) * | 2015-01-28 | 2015-05-20 | 河南省农业科学院植物保护研究所 | Trapping method and device for wheat midges |
-
2015
- 2015-11-25 CN CN201510827268.7A patent/CN105467080B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202907550U (en) * | 2012-09-11 | 2013-05-01 | 鹤壁佳多科工贸有限责任公司 | Intelligent soil excavation device for wheat sitodiplosis mosellana |
| CN104429736A (en) * | 2014-11-13 | 2015-03-25 | 河南省农业科学院植物保护研究所 | Wheat blossom midge soil removal, rinsing and sorting device and use method thereof |
| CN204232011U (en) * | 2014-11-13 | 2015-04-01 | 河南省农业科学院植物保护研究所 | Wheat midge washes in a pan soil, rinsing, sorting equipment |
| CN204241263U (en) * | 2014-12-18 | 2015-04-01 | 河南省农业科学院植物保护研究所 | Wheat midge soil sample barrel |
| CN104621076A (en) * | 2015-01-28 | 2015-05-20 | 河南省农业科学院植物保护研究所 | Trapping method and device for wheat midges |
Non-Patent Citations (1)
| Title |
|---|
| 武予清等: "《麦红吸浆虫的研究与防治》", 30 September 2011, 科学出版社 * |
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