CN105420092A - Cavitation impinging stream microalga cell wall-breaking device - Google Patents
Cavitation impinging stream microalga cell wall-breaking device Download PDFInfo
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- CN105420092A CN105420092A CN201510921809.2A CN201510921809A CN105420092A CN 105420092 A CN105420092 A CN 105420092A CN 201510921809 A CN201510921809 A CN 201510921809A CN 105420092 A CN105420092 A CN 105420092A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
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Abstract
The invention belongs to the field of microalga cell wall breaking, and particularly discloses a cavitation impinging stream microalga cell wall-breaking device and an application method thereof. The cavitation impinging stream microalga cell wall-breaking device comprises a pipeline circulation system, a cavitation impinging stream system and a measuring system. A uniform cavitation strengthening field can be formed by fluid and alga slurry of microalga cells through cavitation impinging within a large range, microjet and instantaneous high temperature and high pressure generated during collapse of cavitation bubbles repeatedly act on cell surfaces to break molecular structures and smash organic matter molecules, and therefore cell walls are broken. According to the cavitation impinging stream microalga cell wall-breaking device, hydrodynamic cavitation and an impinging stream technology are coupled, the instantaneous microjet and shock waves generated during the collapse of the cavitaiton bubbles and strong turbulence and pressure fluctuation formed by impinging, cutting and extrusion of impinging areas are fully utilized, turbulence conditions allowing cavitation to occur are increased through the strong turbulence of the impinging areas to enable cavitation to continue to occur in the impinging areas, the wall-breaking effect is improved greatly, the cavitation impinging stream microalga cell wall-breaking device is simple in structure and low in operating cost, and the industrialization can be achieved easily.
Description
Technical field
The present invention relates to a kind of cavitation percussion flow microalgae cell means for breaking walls, belong to microalgae cell broken wall field.
Background technology
In recent years, along with China's rapid development of economy, global fossil energy worsening shortages and its relevant environment pollution problem developing generation become increasingly conspicuous, and develop clean reproducible biomass energy extremely urgent.Wherein, photosynthetic efficiency is high, adaptive capacity to environment is strong, growth cycle is short because having for algae bio matter, be easy to breeding, the area that do not occupy cultivated land, biomass are large and efficiently consolidate the plurality of advantages such as charcoal, have become one of potential raw material of preparation biofuel.But, in the exploitation process of microalgae biomass, most micro-algaes has tough and tensile cellulosic cell walls, the extraction of block cell grease, and effective extraction of grease significantly can reduce the cost of manufacture of microalgae biodiesel, thus need to carry out cell wall breaking process by some special methods to it.
Cell wall breaking method the most common has high pressure homogenization method, freeze-thaw method, supersonic method, organic solvent method, polishing, microwave heating method and combined-enzyme method etc. at present.But aforesaid method also exists that step is various, restricted application, energy consumption are too high, broken time is long, broken wall inefficiency, not easily realize the problems such as broken wall industrialization.How efficiently broken wall treatment can be carried out to micro-algae raw material, to overcome above-mentioned problems, develop a kind of new and effective cell wall breaking device technology significant.
Cavitation percussion flow has put forward based on Hydrodynamic cavitation and percussion flow two kinds of technology, two strands of cavitation jets carrying a large amount of cavitation bubble are made to flow in opposite directions and clash into, the cavity that cavitation produces can produce TRANSIENT HIGH TEMPERATURE high pressure and microjet repeated action in cell surface when it is crumbled and fall, break molecular structure, organic molecule is smashed, thus makes cell wall breaking.And mutually flow two strands of liquid phases make cell break to producing during shock under strong collision, shearing, extruding etc. interact, thus make cell wall breaking.Simultaneously due to strong collision, the energy of flow of segment fluid flow is converted into vibrational energy, forms strong turbulence at impingement region, increases the turbulent-flow conditions that cavitation occurs, and then makes cavitation phenomenon continue to occur at impingement region, and bubble collapse better effects if, cell-wall breaking ratio is higher.In addition, compared with traditional cell wall breaking, have energy consumption low, device is simple, maintenance cost is low and be easy to the significant advantages such as industrialization.
Although the device at present about cell wall breaking is of a great variety, the device efficiently carrying out cell wall breaking about cavitation coupling percussion flow has no report, thus, the invention discloses a kind of new and effective cavitation percussion flow microalgae cell means for breaking walls.
Summary of the invention
In order to overcome that at present existing wall breaking technology energy consumption is high, efficiency is low and can not the deficiency such as mass-producing amplification; the invention provides a kind of cavitation percussion flow microalgae cell means for breaking walls, this microalgae cell means for breaking walls is simple, maintenance cost is low and be easy to the significant advantages such as industrialization.
Technical scheme of the present invention is:
A kind of cavitation percussion flow microalgae wall breaking device, comprises the pipeline recycle system, cavitation percussion flow system and measuring system; The described pipeline recycle system comprises material box, recycle pump, valve I ~ III; Described cavitation percussion flow system comprises Hydrodynamic cavitation reactor, clashes into room, nozzle; Measuring system comprises under meter, tensimeter;
Described material box is connected by pipeline with the import of recycle pump, and circulation pump outlet is connected with shock room by 2N branch road, clashes into after the outlet of room is connected by pipeline with valve III and gets back to material box, forms a complete closed cycle; Described N >=1;
The pipeline clashing into room that exports to from recycle pump on described branch road sets gradually valve II, under meter, tensimeter, Hydrodynamic cavitation reactor, nozzle; Described nozzle is fixedly connected with shock room, and it is indoor to be symmetrically arranged in shock;
Described circulation pump outlet is connected with to material box by pipeline, and described circulating-pump outlet is provided with valve I to the pipeline of material box.
Described branch road quantity is 4, wherein, and N=2.
Described material box volume is 30L; Circulating pump lift is 45 ~ 60m; The described pipeline recycle system all adopts 316L stainless steel, and specification is Φ 20mm*2mm; Described manometric scope is 0 ~ 0.6MPa, and described flow counts spinner-type flowmeter.
Described Hydrodynamic cavitation reactor is can embedded hole board-like dismountable flange dish cavitation reactor, and ring flange inside nominal diameter is 20mm, and external diameter is 100mm, and thickness is 14mm.
Described orifice plate is single hole or multi-hole orifice, and percentage of open area is 0.02 ~ 0.1.
The hole count of described multi-hole orifice is 5 ~ 20.
A using method for cavitation percussion flow microalgae cell means for breaking walls, specifically comprises the steps:
A, in material box, add the microalgae cell raw material of fluid and non-broken wall, and be uniformly mixed;
B, ON cycle pump, make fluid and microalgae cell mixture be full of whole pipeline by regulated valve II and valve III;
C, fluid and microalgae cell mixture flow, after spinner-type flowmeter and tensimeter, enter Hydrodynamic cavitation reactor, collide subsequently in shock indoor after 2N the nozzle be arranged symmetrically with;
D, the fluid clashing into indoor collision rift and microalgae cell mixture flow into material box after valve III, proceed next circulation.
Described microalgae cell raw material is that the oil-rich microalgae that lipid content is higher maybe can produce the micro-algae of bioactive economy, and described fluid is the good liquid of mobility such as water, methyl alcohol, ethanol.
Described microalgae cell raw material is chlorella, Du Shi diatom, salt algae and flat algae etc.
The time of the fluid described in step C and micro-algae circulating reaction is 10 ~ 30min.
During device work of the present invention, pressure optimum is 0.4MPa,
The invention has the beneficial effects as follows:
(1) compared to traditional mechanical breaking-wall method and ultrasonication, low, the speed of reaction of cavitation percussion flow microalgae cell means for breaking walls energy consumption that the present invention adopts is fast, cell-wall breaking ratio is up to 80%.
(2) the cavitation percussion flow microalgae cell means for breaking walls that adopts of the present invention is simple, and maintenance cost is low, can broken wall continuously, is easy to accomplish scale production.
(3) the cavitation percussion flow microalgae cell means for breaking walls of the present invention's employing is without the need to using any chemical reagent, can not produce pollute environment.
(4) the cavitation percussion flow microalgae cell means for breaking walls of the present invention's employing, take full advantage of instantaneous microjet, shockwave and the impingement region collision that bubble collapse produces, strong turbulence and the pressure surge of shearing and extrude formation, the strong turbulence of impingement region adds the turbulent-flow conditions of cavitation generation, make cavitation continue to occur at impingement region, substantially increase shell-broken effect.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of cavitation percussion flow of the present invention microalgae cell means for breaking walls;
Fig. 2 is the internal structure schematic diagram (for single hole) of Hydrodynamic cavitation reactor;
Fig. 3 is for clashing into room vertical view;
In figure: 1, material box; 2, recycle pump; 3, valve I; 4, valve II; 5, under meter; 6, tensimeter; 7, Hydrodynamic cavitation reactor; 8, room is clashed into; 9, nozzle; 10, valve III.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail.
A kind of cavitation percussion flow of the present invention microalgae cell means for breaking walls as shown in Figure 1, mainly comprises the pipeline recycle system, cavitation percussion flow system and measuring system.The pipeline recycle system comprises material box, recycle pump, valve; Cavitation percussion flow system comprises Hydrodynamic cavitation reactor, clashes into the nozzle of room, carry liquid jet; Measuring system comprises under meter, tensimeter.
Described material box 1 is connected by pipeline with the import of recycle pump 2, the outlet of recycle pump 2 is connected with shock room 8 by 4 branch roads (branch road I, branch road II, branch road III, branch road IV), clash into after the outlet of room 8 is connected by pipeline with valve III 10 and get back to material box 1, form a complete closed cycle;
The pipeline clashing into room 8 that exports to from recycle pump 2 on described branch road sets gradually valve II 4, under meter 5, tensimeter 6, Hydrodynamic cavitation reactor 7, nozzle 9; Described nozzle 9 is fixedly connected with shock room 8, and is symmetrically arranged in shock room 8; Described nozzle is the nozzle with hydrofluidic;
The outlet of described recycle pump 2 is connected with to material box 1 by pipeline, and the pipeline that described recycle pump 2 exports to material box 1 is provided with valve I 3.
Described material box 1 volume is 30L; Recycle pump 2 lift is 50m; The described pipeline recycle system all adopts 316L stainless steel, and specification is Φ 20mm*2mm; Described manometric scope is 0 ~ 0.6MPa, and described flow counts spinner-type flowmeter.
Described Hydrodynamic cavitation reactor 7 is can embedded hole board-like dismountable flange dish cavitation reactor, and ring flange inside nominal diameter is 20mm, and external diameter is 100mm, and thickness is 14mm.The hole count of described orifice plate is 13 holes, aperture 1mm.
A kind of cavitation percussion flow microalgae cell means for breaking walls, specifically comprises the steps:
A, in material box, add the 1Kg chlorella microalgae cell raw material of 20L water and non-broken wall, and be uniformly mixed, form more sparse algae mud mixture;
B, unlatching lift are the recycle pump of 50m, make water and chlorella microalgae cell mixture be full of whole pipeline by regulated valve;
C, regulated valve make overpressure be 0.4MPa, make water and chlorella microalgae cell mixture flow after spinner-type flowmeter and tensimeter simultaneously, enter and embed orifice plate (aperture 1mm, 13 holes) Hydrodynamic cavitation reactor produce cavitation crumbling and fall in pressure recovery region, subsequently clash into indoor through 4 be arranged symmetrically with the nozzle of hydrofluidic after collide, form strong turbulence and pressure surge, make cavitation continue to occur at impingement region.
D, the water clashing into room collision rift and chlorella microalgae cell mixture flow into material box after valve, and proceed next circulation, the reaction times is 30min.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention.All any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a cavitation percussion flow microalgae wall breaking device, is characterized in that, comprises the pipeline recycle system, cavitation percussion flow system and measuring system; The described pipeline recycle system comprises material box (1), recycle pump (2), valve I ~ III; Described cavitation percussion flow system comprises Hydrodynamic cavitation reactor (7), clashes into room (8), nozzle (9); Measuring system comprises under meter (5), tensimeter (6);
Described material box (1) is connected by pipeline with the import of recycle pump (2), the outlet of recycle pump (2) is connected with shock room (8) by 2N branch road, get back to material box (1) after the outlet of clashing into room (8) is connected by pipeline with valve III (10), form a complete closed cycle; Described N >=1;
The pipeline clashing into room (8) that exports to from recycle pump (2) on described branch road sets gradually valve II (4), under meter (5), tensimeter (6), Hydrodynamic cavitation reactor (7), nozzle (9); Described nozzle (9) is fixedly connected with shock room (8), and is symmetrically arranged in shock room (8);
The outlet of described recycle pump (2) is connected with to material box (1) by pipeline, and the pipeline that described recycle pump (2) exports to material box (1) is provided with valve I (3).
2. a kind of cavitation percussion flow microalgae wall breaking device according to claim 1, it is characterized in that, described branch road quantity is 4, wherein, N=2.
3. a kind of cavitation percussion flow microalgae wall breaking device according to claim 1, it is characterized in that, described material box (1) volume is 30L, recycle pump (2) lift is 45 ~ 60m, the described pipeline recycle system all adopts 316L stainless steel, specification is Φ 20mm*2mm, and described manometric scope is 0 ~ 0.6MPa, and flow counts spinner-type flowmeter.
4. a kind of cavitation percussion flow microalgae wall breaking device according to claim 1, it is characterized in that, described Hydrodynamic cavitation reactor (7) is can embedded hole board-like dismountable flange dish cavitation reactor, and ring flange inside nominal diameter is 20mm, external diameter is 100mm, and thickness is 14mm.
5. a kind of cavitation percussion flow microalgae wall breaking device according to claim 4, it is characterized in that, described orifice plate is single hole or multi-hole orifice, and percentage of open area is 0.02 ~ 0.1.
6. a kind of cavitation percussion flow microalgae wall breaking device according to claim 5, it is characterized in that, the hole count of described multi-hole orifice is 5 ~ 20.
7. the using method of a kind of cavitation percussion flow microalgae cell means for breaking walls according to claim 1, is characterized in that, specifically comprise the steps:
A, in material box (1), add the microalgae cell raw material of fluid and non-broken wall, and be uniformly mixed;
B, ON cycle pump (2), make fluid and microalgae cell mixture be full of whole pipeline by regulated valve II (4) and valve III (10);
C, fluid and microalgae cell mixture flow, after spinner-type flowmeter (5) and tensimeter (6), enter Hydrodynamic cavitation reactor (7), collide subsequently in shock indoor after 2N the nozzle (9) be arranged symmetrically with;
D, the fluid clashing into indoor collision rift and microalgae cell mixture flow into material box after valve III (10), proceed next circulation.
8. the using method of a kind of cavitation percussion flow microalgae wall breaking device according to claim 7, it is characterized in that, described microalgae cell raw material is that the oil-rich microalgae that lipid content is higher maybe can produce the micro-algae of bioactive economy, and described fluid is the good liquid of mobility such as water, methyl alcohol, ethanol.
9. the using method of a kind of cavitation percussion flow microalgae wall breaking device according to claim 7, it is characterized in that, described microalgae cell raw material is chlorella, Du Shi diatom, salt algae and flat algae etc.
10. the using method of a kind of cavitation percussion flow microalgae wall breaking device according to claim 7, it is characterized in that, the time of the fluid described in step C and micro-algae circulating reaction is 10 ~ 30min, and during device work of the present invention, pressure is 0.4MPa.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510921809.2A CN105420092A (en) | 2015-12-14 | 2015-12-14 | Cavitation impinging stream microalga cell wall-breaking device |
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| CN201510921809.2A CN105420092A (en) | 2015-12-14 | 2015-12-14 | Cavitation impinging stream microalga cell wall-breaking device |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172703A (en) * | 2016-07-11 | 2016-12-07 | 杨超坤 | A kind of aquatic products special bio antistaling agent process units |
| CN109205693A (en) * | 2018-08-21 | 2019-01-15 | 江苏大学 | A kind of detachable cavitation apparatus |
| CN110436562A (en) * | 2019-09-04 | 2019-11-12 | 中科北联(辽宁)环境科技有限公司 | Hydraulic oscillation device, system and method |
| CN111285449A (en) * | 2018-12-06 | 2020-06-16 | 宁波方太厨具有限公司 | Electromagnetic water treatment device and water heater comprising same |
| CN114540113A (en) * | 2022-03-29 | 2022-05-27 | 山东大学 | Cavitation coupling microbubble flotation microalgae oil extraction system and extraction process |
| CN114804976A (en) * | 2022-03-25 | 2022-07-29 | 中瑞(苏州)资源环境科技有限公司 | One-stop preparation method of soil remediation material based on blue algae, sludge and biochar |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172703A (en) * | 2016-07-11 | 2016-12-07 | 杨超坤 | A kind of aquatic products special bio antistaling agent process units |
| CN109205693A (en) * | 2018-08-21 | 2019-01-15 | 江苏大学 | A kind of detachable cavitation apparatus |
| CN111285449A (en) * | 2018-12-06 | 2020-06-16 | 宁波方太厨具有限公司 | Electromagnetic water treatment device and water heater comprising same |
| CN110436562A (en) * | 2019-09-04 | 2019-11-12 | 中科北联(辽宁)环境科技有限公司 | Hydraulic oscillation device, system and method |
| CN114804976A (en) * | 2022-03-25 | 2022-07-29 | 中瑞(苏州)资源环境科技有限公司 | One-stop preparation method of soil remediation material based on blue algae, sludge and biochar |
| CN114540113A (en) * | 2022-03-29 | 2022-05-27 | 山东大学 | Cavitation coupling microbubble flotation microalgae oil extraction system and extraction process |
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