CN105411790A - Water logging type multi-dimensional control ex-vivo ophthalmostat - Google Patents
Water logging type multi-dimensional control ex-vivo ophthalmostat Download PDFInfo
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- CN105411790A CN105411790A CN201510939041.1A CN201510939041A CN105411790A CN 105411790 A CN105411790 A CN 105411790A CN 201510939041 A CN201510939041 A CN 201510939041A CN 105411790 A CN105411790 A CN 105411790A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61G—TRANSPORT, PERSONAL CONVEYANCES, OR ACCOMMODATION SPECIALLY ADAPTED FOR PATIENTS OR DISABLED PERSONS; OPERATING TABLES OR CHAIRS; CHAIRS FOR DENTISTRY; FUNERAL DEVICES
- A61G13/00—Operating tables; Auxiliary appliances therefor
- A61G13/10—Parts, details or accessories
- A61G13/12—Rests specially adapted therefor; Arrangements of patient-supporting surfaces
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/02—Laboratory benches or tables; Fittings therefor
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Abstract
The invention provides a water logging type multi-dimensional control ex-vivo ophthalmostat. The water logging type multi-dimensional control ex-vivo ophthalmostat is used for solving the problems that an ophthalmostat in the prior art is poor in clamping reliability and is only suitable for being operated in the non-liquid environment. The ophthalmostat comprises a diaphragm, a longitudinal motion bracket, a water tank and a longitudinal motion piston device. A piston cylinder of the longitudinal motion piston device penetrates through a through hole in the bottom of the water tank and is fixed to the water tank in a sealed mode. Multiple blades arranged in a diaphragm fixing ring cavity formed in the top end of the piston cylinder stretch and retract by rotating a poke rod to be used for adjusting the horizontal position of an eyeball sample. The longitudinal motion bracket is fixed to the top end of a piston inside the piston cylinder, spherical arc-shaped concave surfaces of the upper end faces of the piston cylinder and the longitudinal motion bracket coincide with the longitudinal central axis of a fixing ring, and the piston moves up and down by pushing and pulling a piston handle to be used for adjusting the longitudinal position of the eyeball sample. Multi-dimensional stable control over the eyeball sample can be achieved, clamping reliability is high, and the full water logging environment is provided and used for microimaging or other fine operations of the eyeball sample.
Description
Technical field
The invention belongs to technical field of medical instruments, relate to a kind of immersion type multi-value regulation and control in-vitro eyeball fixator, can be used for micro-imaging or other accurate operations of in-vitro eyeball sample.
Background technology
In ophthalmology basic research and clinical care, usually need careful observation and dissect the eyeball of a large amount of animal, the eyeball tissue therefore winning all kinds of toy carries out in vitro micro-imaging, is the requisite step of ophthalmology diagnosis research field.
But eyeball has spherical, soft wet and slippery physiological feature, and the eyeball shape size difference of different animals is comparatively large, and these factors cause carrying out effective fixing very difficulty to eyeball sample in imaging experiment.In order to ensure the reproducibility of imaging results, and reducing the change to its morphological characteristic as far as possible, needing a kind of convenient reliable eyeball fixing means, the position stability of eyeball sample can be remained in imaging process.Meanwhile, to clear observation and the accurate operation of eyeball sample, be unable to do without and high-resolution micro-imaging is carried out to it.High-resolution optical and photoacoustic microscope all need to adopt immersion type object lens, therefore sample must be placed in water environment.And imaging and operation technique need location, visual field and the image acquisition of long period usually, so be eyeball tissue supply normal saline in time, can ensure the stable of sample morphology structure, avoid the irreversible breaking that extraneous dry environment causes.
Whether adjustable according to size, existing in-vitro eyeball fixator can be divided into two classes: a class realizes the fixing of eyeball sample by the combination of two or more parts, but do not possess adjustability; Another kind of is by the object clamping eyeball sample in same level dimension adjustable, is applicable to the eyeball sample of different size.Such as, Chinese patent application Authorization Notice No. is CN202568734U, and the patent that name is called " a kind of in-vitro eyeball fixator ", discloses a kind of in-vitro eyeball fixator, and it is covered by a threaded pressure and be with the pedestal of stud to screw up and fixes eyeball.But the pressure cover of this geomery can only fix the eyeball sample of specific size.In addition, the eyeball be placed in pressure cover limits the flexible adjustment at position, space, adds the difficulty of accurate imaging.Chinese patent application application publication number is CN103310681A, and the patent application that name is called " in-vitro eyeball fixator ", provides a kind of in-vitro eyeball fixator, fixes eyeball by hemispherical groove and four fixing cards.But it only can fix the eyeball of specific size, and easily destroy the anatomical structure of eyeball, limit it in accurate imaging and the application in operating.Only be suitable for the shortcoming of fixing specific large microphthalmia to overcome traditional non-adjustable in-vitro eyeball fixture, researcher has invented adjustable in-vitro eyeball fixator.In-vitro eyeball fixator that these are adjustable mainly passes through the size of alignment jig in same level, to be applicable to the eyeball sample clamping different size.Such as, Chinese patent application mandate publication number is CN203220456U, name is called the patent of " Pop-up eyeball fixture ", disclose a kind of Pop-up eyeball fixture, it fixes eyeball sample by annulus lock tube, but this device only can regulate the position of eyeball sample in the horizontal, the accurate manipulation of eyeball sample lengthwise position cannot be realized.In addition, only clamping scheme in the horizontal easily causes eyeball landing, and reliability is poor.And existing adjustable in-vitro eyeball fixator is all only applicable to on-liquid environment.
Summary of the invention
The object of the invention is to the deficiency overcoming the existence of above-mentioned prior art, propose a kind of immersion type multi-value regulation and control in-vitro eyeball fixator, for the technical problem solving existing adjustable in-vitro eyeball fixator clamping poor reliability and operate under being only applicable to on-liquid environment.
For achieving the above object, technical scheme of the present invention is as follows:
A kind of immersion type multi-value regulation and control in-vitro eyeball fixator, comprises diaphragm 1, vertically moves bracket 2, tank 3 and vertically move piston apparatus 4; Diaphragm 1 comprises poke rod 11, multiple blade 12 and retainer ring 13, multiple blade 12 is evenly arrangement in circumference, and level is arranged in the cavity of retainer ring 13, forms circular space 14, this circular space 14 realizes flexible by rotary dial bar 11, for the eyeball sample of level clamping different size; Through hole 31 is provided with bottom tank 3; Vertically move piston apparatus 4 to be made up of piston cylinder 41, piston 42 and the piston handle 43 that is fixed on piston 42 bottom; Vertically move piston apparatus 4 through through hole 31, and fix with its sealing; Vertically move the top that bracket 2 is fixed on piston 42, and be positioned at the below of circular space 14; Retainer ring 13 is fixed on the top of piston cylinder 41.
Above-mentioned immersion type multi-value regulation and control in-vitro eyeball fixator, the upper surface vertically moving bracket 2 is provided with globular arc concave surface 21, for stable placement eyeball sample.
Above-mentioned immersion type multi-value regulation and control in-vitro eyeball fixator, the full-size of globular arc concave surface 21 horizontal direction is less than the maximum gauge of circular space 14.
Above-mentioned immersion type multi-value regulation and control in-vitro eyeball fixator, the maximum transverse size vertically moving bracket 2 is less than the internal diameter size of piston cylinder 41.
Above-mentioned immersion type multi-value regulation and control in-vitro eyeball fixator, the longitudinal central axis line of circular space 14, globular arc concave surface 21 and piston cylinder 41 overlaps.
Above-mentioned immersion type multi-value regulation and control in-vitro eyeball fixator, through hole 31 is equal with the size of the external diameter of piston cylinder 41, and shape is identical.
Above-mentioned immersion type multi-value regulation and control in-vitro eyeball fixator, the degree of depth of tank 3 inside is greater than the height summation size of the piston cylinder 41 and diaphragm 1 being positioned at tank, for realizing the full water logging environment of eyeball sample.
Compared with prior art, tool has the following advantages in the present invention:
1, multiple blades 12 of the evenly arrangement in circumference are included in the diaphragm 1 of the present invention due to employing, the circular space 14 that these blades are formed can realize flexible flexibly by rotary dial bar 11, can level clamping difformity and the eyeball sample of size, and make the horizontal uniform stressed of eyeball sample, be provided with in the below of circular space 14 simultaneously and vertically move bracket 2 with globular arc concave surface 21, this vertically moves bracket 2 and moves up and down by piston 42 drive, be not only eyeball sample and provide longitudinal holding power, and realize the free adjustment of eyeball height of specimen position, compared with existing in-vitro eyeball fixator, the accurate flexible of multidimensional can be realized, improve convenience, the reliability and stability of clamping eyeball sample.
2, the present invention is owing to vertically moving piston apparatus 4 through the through hole 31 bottom tank 3, and fixes with its sealing; Vertically move the top that bracket 2 is fixed on piston 42, and be positioned at the below of circular space 14; Retainer ring 13 is fixed on the top of piston cylinder 41; The degree of depth of tank 3 inside is greater than the height summation size of the piston cylinder 41 and diaphragm 1 being positioned at tank; Compared with prior art, not only achieve the full water logging environment of eyeball sample, effectively prevent the irreversible breaking that extraneous dry environment causes eyeball sample, and the relative position of eyeball sample and horizontal plane is adjustable flexibly, improve the convenience to eyeball sample imaging location and other accurate operations.
Accompanying drawing explanation
Fig. 1 is overall structure schematic diagram of the present invention;
Fig. 2 is mechanism of diaphragm schematic diagram of the present invention;
Fig. 3 of the present inventionly vertically moves carrier structure schematic diagram;
Fig. 4 is diaphragm of the present invention and the locus structural representation vertically moving bracket;
Fig. 5 of the present inventionly vertically moves piston apparatus structural representation;
Fig. 6 is sink structure schematic diagram of the present invention.
Detailed description of the invention
Below in conjunction with drawings and Examples, technical scheme of the present invention is described in further detail.
With reference to Fig. 1, immersion type multi-value regulation and control in-vitro eyeball fixator, comprises diaphragm 1, vertically moves bracket 2, tank 3 and vertically move piston apparatus 4.Diaphragm 1 comprises poke rod 11, multiple blade 12 and retainer ring 13, multiple blade 12 is evenly arrangement in circumference, level is arranged in the cavity of retainer ring 13, circular space 14 is formed centrally in retainer ring 13, multiple blade 12 radial expansion is made by rotary dial bar 11, realize saving through size continuously adjustabe of circular space 14, be applicable to the eyeball sample of level clamping different size.The upper surface vertically moving bracket 2 is provided with globular arc concave surface 21, for stable placement eyeball sample; The full-size of globular arc concave surface 21 horizontal direction is less than the maximum gauge of circular space 14, can realize vertically moving bracket 2 can partly be deep in retainer ring 13, ensure the maximum transverse size position of less eyeball sample or its slightly upper/lower positions by stable clamping.The bottom of tank 3 is provided with through hole 31, and the shape of this through hole 31 is circular, and is positioned at the bottom centre position of tank 3.Vertically move piston apparatus 4 to be made up of piston cylinder 41, piston 42 and the piston handle 43 that is fixed on piston 42 bottom, piston cylinder 41 adopts transparent plastics material, so that observe piston 42 and the lengthwise position vertically moving bracket 2 in experiment, its shape is cylindrical, internal diameter equals the internal diameter size of retainer ring 13, and external diameter equals the outside dimension of retainer ring 13.Diaphragm 1 is fixed on the top of piston cylinder 41 by marine glue, and keeps retainer ring 13 to overlap with the longitudinal central axis line of piston cylinder 41; The maximum transverse size vertically moving bracket 2 is less than the internal diameter of piston cylinder 41, is fixed on the top of piston 42 by marine glue, and keeps globular arc concave surface 21 to overlap with the longitudinal central axis line of piston cylinder 41; The longitudinal central axis line of retainer ring 13, spherical arc shape concave surface 21 and piston cylinder 41 overlaps, and ensure that the harmony that eyeball sample is stressed when its horizontal and vertical position adjustments.The internal diameter of through hole 31 is equal with the outside dimension of piston cylinder 41; Vertically move piston apparatus 4 and pass perpendicularly through through hole 31, and with fluid sealant, the junction of piston cylinder 41 with through hole 31 is sealed, and then anti-sealing oozes out.
With reference to Fig. 2, diaphragm 1 is stainless steel, comprises poke rod 11, multiple blade 12 and retainer ring 13, and multiple blade 12 is evenly arrangement in circumference, and level is arranged in the cavity of retainer ring 13, is formed centrally circular space 14 in retainer ring 13.By rotary dial bar 11, can drive the flexible of multiple blade 12, when poke rod 11 rotates by direction shown in solid arrow, multiple blade 12 stretches to the center of circular space 14, and circular space 14 diminishes; When poke rod 11 reversely rotates by dotted arrow, multiple blade 12 shrinks away from circular space 14 center, and circular space 14 becomes large; The change of circular space 14 size is applicable to the eyeball sample clamping different size, and the different longitudinal position of same eyeball sample.In the present embodiment use the thickness of multiple blades 12 of diaphragm 1 to be 2mm; The shape of retainer ring 13 is annular, and its internal diameter size is 20mm, and outside dimension is 25.4mm, and thickness is 5mm; The maximum gauge of circular space 14 is 20mm.
With reference to Fig. 3, vertically move bracket 2 and adopt stainless steel, its shape is cylindrical.In the present embodiment, bracket 2 height dimension that vertically moves used is 20mm, the globular arc concave surface 21 of its upper surface overlaps with the longitudinal central axis line vertically moving bracket 2, and the full-size of globular arc concave surface 21 horizontal direction is equal with the trans D vertically moving bracket 2, size is 19.5mm, and the summit of spherical arc shape concave surface is 4mm to the distance of horizontal plane.
With reference to Fig. 4, diaphragm 1 is placed in and cylindrically vertically moves above bracket 2, and circular space 14 keeps overlapping with the longitudinal central axis line of globular arc concave surface 21.Eyeball adopts following modes of emplacement: first by the poke rod 11 of dotted arrow rotary diaphragm 1, regulate circular space 14 to maximum through size, eyeball is positioned over by circular space 14 and vertically moves on bracket 2, and keep the minimum point of eyeball to overlap with the minimum point of globular arc concave surface 21; Secondly press solid arrow rotary dial bar 11, slowly stretch multiple blade 12, regulate the size of circular space 14 to clamp eyeball sample, make it keep stable.Because multiple blades 12 of diaphragm 1 evenly stretch in the horizontal plane, ensure that the uniform stressed of eyeball lateral attitude; Meanwhile, the good laminating in the bottom surface of globular arc concave surface 21 and eyeball bottom, for eyeball provides good longitudinal bracing, therefore puts on horizontal and vertical holding power and ensure that eyeball sample is clamped by stable.
With reference to Fig. 5, vertically move piston apparatus 4 and be made up of piston cylinder 41, piston 42 and the piston handle 43 that is fixed on piston 42 bottom.Piston cylinder 41 adopts transparent plastics material, and its internal diameter is greater than the cylindrical diameter vertically moving bracket 2, equals the diameter of piston 42, and external diameter equals the external diameter of the retainer ring 13 of diaphragm 1.Diaphragm 1, the connected mode that vertically moves bracket 2 and vertically move piston apparatus 4 be as follows: first, uses marine glue to vertically move bracket 2 and be firmly fixed on the top of piston 42; Secondly, use marine glue diaphragm 1 to be fixed on closely the top of piston cylinder 41, this bonding way maintains the accurate coincidence of circular space 14, globular arc concave surface 21 and piston cylinder 41 three longitudinal central axis line.Because the diameter of piston 42 is equal with the internal diameter of piston cylinder 41, the squeezed tight effect of both utilizations, can avoid the loss of water.The adjustment of eyeball height of specimen position is realized as follows: push-and-pull piston handle 43, drive piston 42 to move up and down with the assembly vertically moving bracket 2; Utilize the transparent characteristic of piston cylinder 41 in the process, the lengthwise position of Real Time Observation sample, treat that eyeball moves to suitable height, stop push-and-pull piston handle 43; The poke rod 11 of rotary diaphragm 1 regulates the size of circular space 14, and stable clamps eyeball sample.In the present embodiment, piston cylinder 41 internal diameter size is 20mm, and outside dimension is 25.4mm, and height dimension is 80mm.
With reference to Fig. 6, tank 3 adopts clear glass material, and shape is cylindrical, and its bottom centre position is provided with manhole 31.The degree of depth of tank 3 inside is greater than the height summation size of the piston cylinder 41 and diaphragm 1 being positioned at tank, to guarantee that the horizontal plane after injecting water can dipped whole eyeball.Tank 3 diameter adopted in the present embodiment is 50.8mm, and internal depth is of a size of 30mm; The diameter of through hole 31 is 25.4mm.
More than describe and embodiment; be only preferred embodiment of the present invention; do not form any limitation of the invention; obviously for those skilled in the art; after having understood content of the present invention and design principle; all when based on principle of the present invention and structure, the various correction in form and in details and change may be carried out, but these corrections based on inventive concept and change are still within the protection domain of claim of the present invention.
Claims (7)
1. an immersion type multi-value regulation and control in-vitro eyeball fixator, is characterized in that: comprise diaphragm (1), vertically move bracket (2), tank (3) and vertically move piston apparatus (4); Described diaphragm (1) comprises poke rod (11), multiple blade (12) and retainer ring (13), multiple blade (12) is evenly arrangement in circumference, level is arranged in the cavity of retainer ring (13), form circular space (14), this circular space (14) realizes flexible by rotary dial bar (11), for the eyeball sample of level clamping different size; Described tank (3) bottom is provided with through hole (31); The described piston apparatus (4) that vertically moves is made up of piston cylinder (41), piston (42) and the piston handle (43) that is fixed on piston (42) bottom; The described piston apparatus (4) that vertically moves through through hole (31), and is fixed with its sealing; Describedly vertically move the top that bracket (2) is fixed on piston (42), and be positioned at the below of circular space (14); Described retainer ring (13) is fixed on the top of piston cylinder (41).
2. immersion type multi-value regulation and control in-vitro eyeball fixator according to claim 1, is characterized in that: described in vertically move bracket (2) upper surface be provided with globular arc concave surface (21), for stable placement eyeball sample.
3. immersion type multi-value regulation and control in-vitro eyeball fixator according to claim 2, is characterized in that: the full-size of described globular arc concave surface (21) horizontal direction is less than the maximum gauge of circular space (14).
4. immersion type multi-value regulation and control in-vitro eyeball fixator according to claim 1, is characterized in that: described in vertically move bracket (2) maximum transverse size be less than the internal diameter size of piston cylinder (41).
5. immersion type multi-value regulation and control in-vitro eyeball fixator according to claim 1 and 2, is characterized in that: the longitudinal central axis line of described circular space (14), globular arc concave surface (21) and piston cylinder (41) overlaps.
6. immersion type multi-value regulation and control in-vitro eyeball fixator according to claim 1, is characterized in that: described through hole (31) is equal with the size of the external diameter of piston cylinder (41), and shape is identical.
7. immersion type multi-value regulation and control in-vitro eyeball fixator according to claim 1, it is characterized in that: the degree of depth of described tank (3) inside is greater than the height summation size of piston cylinder (41) and the diaphragm (1) being positioned at tank, for realizing the full water logging environment of eyeball sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510939041.1A CN105411790A (en) | 2015-12-15 | 2015-12-15 | Water logging type multi-dimensional control ex-vivo ophthalmostat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510939041.1A CN105411790A (en) | 2015-12-15 | 2015-12-15 | Water logging type multi-dimensional control ex-vivo ophthalmostat |
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| CN105411790A true CN105411790A (en) | 2016-03-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510939041.1A Pending CN105411790A (en) | 2015-12-15 | 2015-12-15 | Water logging type multi-dimensional control ex-vivo ophthalmostat |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110542548A (en) * | 2019-08-27 | 2019-12-06 | 杭州电子科技大学 | Soft arthropod robot joint testing device and testing method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0824284A (en) * | 1994-07-14 | 1996-01-30 | Nippon Medoko:Kk | Eye ball holder for experiment |
| CN202568734U (en) * | 2012-05-06 | 2012-12-05 | 中国人民解放军南京军区南京总医院 | Isolated eyeball fixer |
| CN103310681A (en) * | 2013-07-01 | 2013-09-18 | 中国人民解放军总医院 | In-vitro eyeball fixator |
| CN204501481U (en) * | 2015-03-03 | 2015-07-29 | 天津开发区合普工贸有限公司 | A kind of sizable in-vitro eyeball fixture |
-
2015
- 2015-12-15 CN CN201510939041.1A patent/CN105411790A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0824284A (en) * | 1994-07-14 | 1996-01-30 | Nippon Medoko:Kk | Eye ball holder for experiment |
| CN202568734U (en) * | 2012-05-06 | 2012-12-05 | 中国人民解放军南京军区南京总医院 | Isolated eyeball fixer |
| CN103310681A (en) * | 2013-07-01 | 2013-09-18 | 中国人民解放军总医院 | In-vitro eyeball fixator |
| CN204501481U (en) * | 2015-03-03 | 2015-07-29 | 天津开发区合普工贸有限公司 | A kind of sizable in-vitro eyeball fixture |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110542548A (en) * | 2019-08-27 | 2019-12-06 | 杭州电子科技大学 | Soft arthropod robot joint testing device and testing method |
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Application publication date: 20160323 |