CN105410454B - A kind of adjusting control agent based on licorice, and the preparation method and application thereof - Google Patents
A kind of adjusting control agent based on licorice, and the preparation method and application thereof Download PDFInfo
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- CN105410454B CN105410454B CN201510843107.7A CN201510843107A CN105410454B CN 105410454 B CN105410454 B CN 105410454B CN 201510843107 A CN201510843107 A CN 201510843107A CN 105410454 B CN105410454 B CN 105410454B
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- control agent
- licorice
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention belongs to animal-breeding, nutrition regulation and disease prevention technical field, especially a kind of adjusting control agent based on licorice, and the preparation method and application thereof.Adjusting control agent composition is as follows：1-3 parts of licorice, 2-4 parts of potassium chloride, 1-3 parts of citric acid, 1-3 parts of ethoxyquinoline, 1-3 parts of zeolite powder.Above-mentioned raw materials are crushed to 120 mesh respectively when preparation, are sufficiently mixed, 23-27 DEG C is placed in and stirs 24 hours, obtain enoxolone；It is 1~2.5 by enoxolone and glycyrrhizic acid ratio:1~1.5 mixture, then be sufficiently mixed with 2-4 parts of potassium chloride, it is placed in room temperature environment lower 24 hours or more, obtains pale white mixture, it can be as fish regulation of fat agent.Improvement of the invention substitutes enoxolone sterling with licorice, substitutes sodium chloride with potassium chloride, further increases fish body immunity from many aspects.
Description
Technical field
The invention belongs to animal-breeding, nutrition regulation and disease prevention technical fields, and especially one kind is with licorice
Based on adjusting control agent, and the preparation method and application thereof.
Background technique
Studies have shown that in current aquaculture process, since feed nutrition element is uneven, the excessive fat of feed addition
The problems such as with energy, water quality deterioration, various stressors, causes cultured fishes liver, visceral fat deposition to increase, and muscle fat contains
Amount variation, causes fatty,fiss liver to take place frequently, and harvesting rate reduces, immunity degradation, and anti-stress ability decline, survival rate reduces, fish
Meat decline etc., has seriously affected the sound development of culture fishery and the supply of green aquatic product.Meanwhile part raiser
To reduce loss, antibiotic, fishing medicine abuse cause it in the intracorporal residual of fish, cause secondary hazards to listing aquatic products, also sternly
The foreign exchange earning for affecting special aquatic products product again brings massive losses to China's fishery economic.Therefore, cultivation fish how is solved
The problem of class fat over-deposit, it has also become the research hotspot problem of interest of aquaculture at present.
Regulation of fat refers to adds certain regulation of fat substance in fish feed, regulates and controls fish body fat metabolism and transhipment,
The technology of excessively high liver, visceral adipose is reduced.Research has shown that regulation of fat substance can regulate and control body from many aspects
Interior fat metabolism and transhipment.Currently, regulation and control substance used in cultivation mainly includes two classes：One kind is that liver fat is promoted to turn
Fortune, including choline, glycine betaine, phosphatide etc., the activity of main carrier or enzyme by increasing liver fat transhipment, promote liver rouge
Fat transports out liver.The characteristics of this substance is：The health of fish body is not influenced, itself is the indispensable nutriment of fish
One of, it will lead to liver when lacking and fatization phenomenon occur.It is effective to be unsatisfactory for addition when requirement, but works as and meets requirement
Afterwards, it additionally adds typically no effect or effect is not significant.Such regulation of fat agent does not reduce the fat deposited in internal organ
Effect.Another kind of is antioxidant, and fat metabolism in liver or adipose tissue needs to carry out β~oxidation, works as hyperliposis
When, body causes interior free yl to be accumulated because of a large amount of β~oxidation process of progress, and the peroxide such as malonaldehyde largely generate,
Cause internal antioxidant system disorder, causes catabolism of fat speed to decline, accumulation of fat.Fish body takes in antioxidant (such as
VC, VE etc.) after, extra free radical is eliminated, peroxide of degrading alleviates fat to promote liver fat catabolism
Deposition.The characteristics of such additives is：There is certain growth promotion while alleviating fatty excessive accumulation and improves immune masterpiece
With, but act on small on reducing Liver fatty deposition, deposited lipolysis or facilitation cannot be promoted unknown
It is aobvious.Such regulation of fat agent can not reduce the fat deposited in internal organ.
Currently, in aquaculture field, still fish can be effectively inhibited to generate liver, visceral fat deposition without one kind,
And to the adjusting control agent that the fatty,fiss for having generated liver, visceral fat deposition is degraded.Reinforce the basic research of fatty,fiss metabolism,
Exploitation is suitble to the regulation of fat agent of production, has positive realistic meaning to the production of environmentally protective aquatic products.
Radix Glycyrrhizae is " King of Herbs ", and extract enoxolone is its main active principle.Currently, domestic, there has been no about sweet
Careless hypo acid is applied to the report and Related product of fatty,fiss regulation, less to the research of enoxolone, only in weanling pig
There is a small amount of research in substitute breast milk.
In the prior art, application No. is the patents of invention of 201110133844.X to disclose a kind of fish body fat depositing regulation
Combination and application, mainly using enoxolone, but have the following problems in use：
（1）In the breeding process, often concomitant immunity power declines fatty,fiss liver, only regulates and controls rouge with enoxolone sterling
Fat cannot enhance cultured fishes immunity, influence on survival rate little；
（2）Single use enoxolone, enoxolone sterling price is high, and source is unstable, leads to production cost and cultivation
High expensive, economic benefit is low, is unfavorable for the sustainable development of raiser.
Summary of the invention
In order to overcome the shortcomings of existing technology, the adjusting control agent that the present invention provides a kind of based on licorice and
Preparation method and application.
The technical solution adopted by the present invention to solve the technical problems is：A kind of fish fat based on licorice
Adjusting control agent forms as follows：1-3 parts of licorice, 2-4 parts of potassium chloride, 1-3 parts of citric acid, 1-3 parts of ethoxyquinoline, boiling
1-3 parts of mountain flour.
Adjusting control agent of the present invention further comprises that the licorice presses enoxolone：Glycyrrhizic acid is 1~2.5:1~
1.5。
A kind of preparation method of the regulation of fat agent of the fish based on licorice, method are as follows：
1）100.0 g of Radix Glycyrrhizae chopping is weighed, by solid-to-liquid ratio 1：6.5~8.5 ratio is incorporated as 10% containing ammonia 0.50%
800.00 g of mass fraction ethanol water, twice in 80 DEG C of refluxing extractions, flask of the Soxhlet extraction device not less than 1L, often
Secondary 2 h, filtering, vacuum pump filters, with the filter cloth of appropriate specification；
2）Merge extracting solution twice, is concentrated in vacuo to the 1/8~1/10 of original volume, is added dropwise with vigorous stirring through diluting
The concentrated sulfuric acid GR V crossed：Deionized water V=1：1, pH to 1.5~2.0 is adjusted, 5h is placed, is filtered under diminished pressure, pH is washed with water in filter cake
To 3.5~4.0, dry crude product 1；
3）100 ml of aqueous solution of 6~8% sulfuric acid is added in the crude product 1 that above-mentioned steps are extracted, under condition of normal pressure, in 100
It DEG C is heated to boiling, 12 h of back flow reaction, with being vigorously stirred；
4）Said mixture is let cool, is filtered, is then washed with 10 mL deionized waters and is deposited to pH and does not change, then will consolidated
Body is dried in 50 DEG C, obtains taupe Radix Glycyrrhizae crude extract；
5）By above-mentioned licorice 1-3 parts, 2-4 parts of potassium chloride, 1-3 parts of citric acid, 1-3 parts of ethoxyquinoline, zeolite
1-3 parts of powder are crushed to 120 mesh respectively, are sufficiently mixed, and are placed in 23-27 DEG C and stir 24 hours, obtain enoxolone；By enoxolone
It is 1~2.5 with glycyrrhizic acid ratio:1~1.5 mixture, then be sufficiently mixed with 2-4 parts of potassium chloride, it is placed in 24 under room temperature environment
Hour or more, pale white mixture is obtained, it can be as fish regulation of fat agent.
Preparation method of the present invention further comprises, the citric acid as acidulant act on this adjusting control agent 24 hours or more,
Ethoxyquinoline as reducing agent act on this adjusting control agent 24 hours or more, potassium chloride as K+ donor, zeolite powder as dispersing agent.
A kind of purposes of the regulation of fat agent of the fish based on licorice, in fish body interior fat lipid-loweringing fish meal
Application.
Another embodiment of purposes according to the present invention further comprises that adjusting control agent presses 0.3 in the raw material of the fish meal
~0.5g/kg ratio is added.
Another embodiment of purposes according to the present invention further comprises that the fish meal raw material includes：Fish meal, dregs of beans,
Rapeseed meal, cotton dregs, soya-bean oil, flour, wheat bran, calcium dihydrogen phosphate, compound premix and adjusting control agent.
The beneficial effects of the invention are as follows：Glycyrrhizic acid extract of the present invention is aided with citric acid, second as main active principle
Uniform regulation of fat agent is made in phenoxyl quinoline, zeolite powder, potassium chloride；Enoxolone and Radix Glycyrrhizae acid blend, by preparation and
After test, has and adjust fat well and reduce interior fat effect, can effectively improve fatty,fiss metabolism, aquatic products can be enhanced
Animal health, improvement of the invention substitute enoxolone sterling with licorice, substitute sodium chloride with potassium chloride, grow to fish
Without negative effect, fish body immunity is further enhanced from many aspects, improves the survival rate of fish body；In addition, licorice substitutes
Enoxolone sterling can eliminate the disadvantage that enoxolone sterling price is high, source is unstable, reduce production cost, improve water
Produce the comprehensive benefit of cultivation.
Detailed description of the invention
Fig. 1 is influence diagram of the adjusting control agent to megalobrama amblycephala rate of body weight gain.
Fig. 2 is influence diagram of the adjusting control agent to megalobrama amblycephala abdominal fat.
Fig. 3 is the influence diagram that adjusting control agent attacks survival rate after poison to megalobrama amblycephala.
Fig. 4 is influence diagram of the adjusting control agent to megalobrama amblycephala hepatic fat content.
Specific embodiment
The present invention will be further explained with reference to the examples below.
One, adjusting control agent and its preparation
Then the main component that the present invention is with licorice is aided with citric acid, ethoxyquinoline, zeolite powder, chlorination
Uniform regulation of fat agent is made in potassium.
The preparation process of licorice is as follows：
1）100.0 g of Radix Glycyrrhizae chopping is weighed, by solid-to-liquid ratio 1：6.5~8.5 ratio is incorporated as 10% containing ammonia 0.50%
800.00 g of mass fraction ethanol water, twice in 80 DEG C of refluxing extractions, flask of the Soxhlet extraction device not less than 1L, often
Secondary 2 h, filtering, vacuum pump filters, with the filter cloth of appropriate specification；
2）Merge extracting solution twice, is concentrated in vacuo to the 1/8~1/10 of original volume, is added dropwise with vigorous stirring through diluting
The concentrated sulfuric acid GR V crossed：Deionized water V=1：1, pH to 1.5~2.0 is adjusted, 5h is placed, is filtered under diminished pressure, pH is washed with water in filter cake
To 3.5~4.0, dry crude product 1；
3）100 ml of aqueous solution of 6~8% sulfuric acid is added in the crude product 1 that above-mentioned steps are extracted, under condition of normal pressure, in 100
It DEG C is heated to boiling, 12 h of back flow reaction, with being vigorously stirred；
4）Said mixture is let cool, is filtered, is then washed with 10 mL deionized waters and is deposited to pH and does not change, then will consolidated
Body is dried in 50 DEG C, taupe Radix Glycyrrhizae crude extract is obtained, as adjusting control agent main component.
Wherein, licorice presses enoxolone：Glycyrrhizic acid is 1-2.5:1-1.5.
After obtaining Radix Glycyrrhizae crude extract, it can carry out preparing adjusting control agent of the invention, method is as follows：By above-mentioned Radix Glycyrrhizae
1-3 parts of extract, 2-4 parts of potassium chloride, 1-3 parts of citric acid, 1-3 parts of ethoxyquinoline, 1-3 parts of zeolite powder be crushed to 120 respectively
Mesh is sufficiently mixed, and is placed in 23-27 DEG C and is stirred 24 hours, obtains enoxolone；By enoxolone and glycyrrhizic acid ratio be 1~
2.5:1~1.5 mixture, then be sufficiently mixed with 2-4 parts of potassium chloride, it is placed in room temperature environment lower 24 hours or more, obtains canescence
Mixture, can be as fish regulation of fat agent.
The embodiment 1 of adjusting control agent of the present invention is as follows：1 part of licorice, 4 parts of potassium chloride, 1 part of citric acid, ethoxyquin
1 part of quinoline, 1 part of zeolite powder.
Two, the application of adjusting control agent
Adjusting control agent of the invention, which can be applied to preparation, reduces the fish meal of fish body fat, and arbitrary fish meal can will be adjusted
Agent is controlled to be added in the ratio of 0.3-0.5g/kg.
The embodiment of the present invention 1：
Fish meal component：5 parts of fish meal, 30 parts of dregs of beans, 12.5 parts of rapeseed meal, 15 parts of cotton dregs, 2.26 parts of soya-bean oil, 0 part of flour, bran
4.5 parts of skin, 1.8 parts of calcium dihydrogen phosphate, 1 part of compound premix and 2 parts of adjusting control agent.These components are mixed sufficiently, fish is made and raises
Material.The adjusting control agent used is the formula of above-mentioned adjusting control agent embodiment 1.
The experimental analysis of the embodiment of the present invention 1：For the validity for verifying this feed, spy carries out present case, present case setting
Control group（Chow diet）, GA sterling group（Prior art enoxolone group）, this adjusting control agent group.Table 1 is test each group raw material
Formula table, wherein control group is commodity material；GA sterling group is the commodity material added with GA sterling, and GA sterling is substituted flour,
The content of GA sterling is 0.2 g/kg in feed formula；Adjusting control agent group is the commodity material added with adjusting control agent, and adjusting control agent is substituted
Flour, the content of adjusting control agent is 20 g/kg in feed formula.In this test recipe, enoxolone and regulation in GA sterling group
Glycyrrhizic acid is consistent with enoxolone total amount in group.
Three groups of fish meal component situation such as the following table 1 of present case：
Three groups of test point：
1, control group：Formula is seen the above table.
2, GA sterling group：0.2 g/kg substitutes flour.
3, this adjusting control agent group：20 g/kg substitute flour.
The experimentation of embodiment 1 and control group is as follows:
1, test fish and aquaculture management
In Nanjing Pukou base, subjects are megalobrama amblycephala in the cultivation place of outdoor breeding, and cage culture is tested and is divided into 3
A group, respectively control group, GA sterling group and adjusting control agent group, every group of 4 repetitions, every to repeat to place 30 tail megalobrama amblycephalas, experiment is opened
Beginning individual weight 80g or so, the culture-cycle 60 days.Control group feeds commercial feed（It is commercially available, feed nutrition component：Crude protein>30,
Crude fat>5, Huaian the earth animal health-care product Co., Ltd）；GA sterling group feeds the commercial feed with GA sterling substitution flour,
Wherein the content of GA sterling is 0.2g/kg（Feed nutrition component：Crude protein>30, crude fat>5, Huaian the earth animal health-care product
Co., Ltd）；Adjusting control agent group feeds the commercial feed with adjusting control agent substitution flour, wherein the 20g/kg of the content of adjusting control agent（It raises
Material nutrient component：Crude protein>30, crude fat>5, Huaian the earth animal health-care product Co., Ltd）.It is observed and recorded during test each
The morbidity and death condition of group subjects, compare the economic benefit of each group after the test.Test arrangement is in high temperature season（7,
August part）It carries out, the specific grouping situation of test is as shown in table 2：
After the test, the rate of body weight gain, abdominal fat of fish body in three groups are measured respectively, attack survival rate and liver fat after poison
Content, and according to this four groups of parameters judge adjusting control agent to the improvement of fish body economic benefits indicator and fish body fat deposition,
Fig. 1-Fig. 4 is specific data analysis chart.
The effect that adjusting control agent of the present invention is significantly improved to weight gain rate, from figure 1 it appears that the increasing of adjusting control agent group
Rate is significantly higher than control group and GA sterling group again, and the rate of body weight gain of GA sterling group is low compared with control group instead.
The effect that adjusting control agent of the present invention is significantly improved to the deposition of abdominal fat, from figure 2 it can be seen that adjusting control agent group and
The abdominal fat of GA sterling group is substantially less than control group, and the abdominal fat of adjusting control agent group is lower than GA sterling group.
Adjusting control agent of the present invention can significantly improve attack poison after fish body survival rate, from figure 3, it can be seen that adjusting control agent group is attacked
The survival rate of subjects is significantly higher than control group and GA sterling group after poison.
The hepatic fat content of fish body can be significantly reduced in the adjusting control agent group, figure 4, it is seen that adjusting control agent group and GA
The hepatic fat content of sterling group is substantially less than control group, and the hepatic fat content of adjusting control agent group is lower than GA sterling group.
Experiment results proved, the adjusting control agent can significantly improve the growth rate of cultured fishes, reduce fish body fat deposition, and
The use cost of adjusting control agent group is substantially less than GA sterling group, and overall economic efficiency is higher, ensure that the inexpensive booming income of cultivation.
Claims (2)
1. a kind of regulation of fat agent of the fish based on licorice, characterized in that its composition is as follows：Licorice 1-3
Part, 2-4 parts of potassium chloride, 1-3 parts of citric acid, 1-3 parts of ethoxyquinoline, 1-3 parts of zeolite powder, in the licorice, Radix Glycyrrhizae
Hypo acid：Glycyrrhizic acid ratio is 1~2.5:1~1.5.
2. the fish according to claim 1 based on licorice is dropped with regulation of fat agent in preparation fish body interior fat
The application of rouge fish meal.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510843107.7A CN105410454B (en) | 2015-11-27 | 2015-11-27 | A kind of adjusting control agent based on licorice, and the preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510843107.7A CN105410454B (en) | 2015-11-27 | 2015-11-27 | A kind of adjusting control agent based on licorice, and the preparation method and application thereof |
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| CN105410454A CN105410454A (en) | 2016-03-23 |
| CN105410454B true CN105410454B (en) | 2018-11-23 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101744122A (en) * | 2009-12-28 | 2010-06-23 | 浙江科盛饲料股份有限公司 | Feed additive, feed and preparation method thereof for preventing nutritional fatty liver of fish |
| CN101779734A (en) * | 2010-03-30 | 2010-07-21 | 合肥华仁农牧集团 | Composite feed additive for preventing and curing liver and gallbladder syndromes of fishes |
| CN102178063A (en) * | 2011-05-23 | 2011-09-14 | 南京农业大学 | Fish fat deposition regulating and controlling composition and application thereof |
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2015
- 2015-11-27 CN CN201510843107.7A patent/CN105410454B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101744122A (en) * | 2009-12-28 | 2010-06-23 | 浙江科盛饲料股份有限公司 | Feed additive, feed and preparation method thereof for preventing nutritional fatty liver of fish |
| CN101779734A (en) * | 2010-03-30 | 2010-07-21 | 合肥华仁农牧集团 | Composite feed additive for preventing and curing liver and gallbladder syndromes of fishes |
| CN102178063A (en) * | 2011-05-23 | 2011-09-14 | 南京农业大学 | Fish fat deposition regulating and controlling composition and application thereof |
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| CN105410454A (en) | 2016-03-23 |
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