CN105410030B - The method that powdery mildew infects in the prevention and treatment tobacco flue-curing of foliage-spray microbial bacterial agent - Google Patents
The method that powdery mildew infects in the prevention and treatment tobacco flue-curing of foliage-spray microbial bacterial agent Download PDFInfo
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- CN105410030B CN105410030B CN201511019043.5A CN201511019043A CN105410030B CN 105410030 B CN105410030 B CN 105410030B CN 201511019043 A CN201511019043 A CN 201511019043A CN 105410030 B CN105410030 B CN 105410030B
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- powdery mildew
- bacillus subtilis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses the methods that powdery mildew in a kind of prevention and treatment tobacco flue-curing of foliage-spray microbial bacterial agent infects, the microbial bacterial agent is bacillus subtilis microbial agent, bacillus subtilis microbial agent is sprayed in powdery mildew morbidity tobacco leaf upper and lower surface to period before baking after picking, the spraying concentration range of microbial inoculum is 50,000,000,000 spore L‑1~3,000 hundred million spore L‑1, toasted after blade dries.The processing method of tobacco leaf before the present invention changes bacillus subtilis insecticide spraying time and toasts, by test of many times the result shows that the method can effectively prevent infecting for Powdery Mildew in Tobacco Leaf Curing, and test out when barn maximum temperature reaches 68 DEG C, bacillus subtilis residual rate is lower than 20%.Compared with routinely adopting and baking, after spraying bacillus subtilis, controlling powdery mildew effect is more significant, and method is simple to operation, low in cost, easy to spread.The present invention provides a kind of new ways that can be used in baking process preventing and treating powdery mildew.
Description
Technical field
The invention belongs to pesticide control technical fields, and in particular to a kind of foliage-spray microbial bacterial agent prevention and treatment tobacco flue-curing
The method that middle powdery mildew infects.
Background technique
Powdery Mildew in Tobacco (Tobacco powdery mildew) is by Powdery Mildew in Tobacco bacterium (Erysipheci
Choracearum DC.) cause.Position of disease is spread from bottom to top usually since inferior leads, and blade is all de- when serious
It falls, only stays vein, and then lead to flue-cured tobacco Severe Reduction.Color is in sepia after by the modulation of disease infestation tobacco leaf, and blade surface has
Apparent dotted scab, show as blade is thin, fragrance there is a serious shortage of, oil is few, damaged degree is big, miscellaneous gas weight, broken rate are high, industry
Poor availability.
Studies have shown that in baking process, as the raising of tobacco leaf temperature and the evaporation of moisture once reach tobacco white powder
The temperature and humidity of pathogen growth, Powdery Mildew can infect tobacco leaf in the stages such as scattered leaf stacking stage and fresh cigarette dress a heatable brick bed, cause
Powdery mildew diffusion.Therefore there is an urgent need to find one can safely and effectively prevent and treat the method that tobacco powdery mildew barn infects.
Summary of the invention
In view of this, infected the present invention provides powdery mildew in a kind of prevention and treatment tobacco flue-curing of foliage-spray microbial bacterial agent
Method, processing method of this method by tobacco leaf before changing the insecticide spraying time of bacteria agent bacillus subtilis and toasting, peace
Infecting for Powdery Mildew in Tobacco Leaf Curing has been effectively prevented entirely.
The technical solution adopted by the present invention is as follows:
1, the method that powdery mildew infects in foliage-spray microbial bacterial agent prevention and treatment tobacco flue-curing, includes the following steps: to pick
Bacillus subtilis microbial agent is sprayed in powdery mildew morbidity tobacco leaf upper and lower surface to before toasting afterwards, the spraying concentration range of microbial inoculum is
50000000000 spore L-1~3,000 hundred million spore L-1, toasted after blade dries.
Preferably, the bacillus subtilis microbial agent spraying concentration is 100,000,000,000 spore L-1。
Preferably, the maximum temperature of tobacco flue-curing is 68 DEG C.
Preferably, fresh tobacco leaf is stacked and carries out bacillus subtilis microbial agent again within 4 hours and spray after tobacco leaf picking.
The beneficial effects of the present invention are: bacillus subtilis is one kind of phyllospheric microganism, under barn hot environment
It remains to continue to breed, the present invention before tobacco flue-curing by spraying bacillus subtilis microbial agent, to white powder in Tobacco Leaf Curing
Germ, which is infected, to be effectively prevented, and when barn maximum temperature reaches 68 DEG C, and bacillus subtilis residual rate is lower than 20%.It should
Microbial bacterial agent is cheap, and dilution process and operating method are simple.Compared with routinely adopting and baking, after spraying bacillus subtilis,
Controlling powdery mildew effect is more significant, and method is simple to operation, low in cost, easy to spread.In addition, bacillus subtilis
Nontoxic no agriculture is residual, environmentally safe.The present invention provides a kind of new ways for preventing and treating powdery mildew.
Detailed description of the invention
In order to keep the purpose of the present invention, technical scheme and beneficial effects clearer, the present invention provides following attached drawing:
Fig. 1 different agents processing baking front and back disease index comparing result；
Contrast on effect result is prevented and treated after the processing baking of Fig. 2 different agents.
Specific embodiment
A preferred embodiment of the present invention will be described in detail below.The dilution side of actual conditions is not specified in embodiment
Method and experimental method, usually according to conventional conditions or according to the manufacturer's recommendations.
The method for preventing and treating Powdery Mildew Secondary Infection in Tobacco Leaf Curing, includes the following steps: bacillus subtilis bacterium
50,000,000,000 spore L are pressed in agent-1~3,000 hundred million spore L-1Concentration dilution (the bacillus subtilis microbial agent dosage form is unlimited), Yu Cai
Period sprays in powdery mildew morbidity tobacco leaf upper and lower surface to before toasting after plucking, and blade enters roasting after being put at gravity-flow ventilation until drying
Baking room.
Embodiment 1
Provided in this embodiment is method 1, and bacillus subtilis microbial agent is applied by following concentration and time: bacillus subtilis
Bacterium wettable powder is diluted to 50,000,000,000 spore L-1；Rear fresh tobacco leaf interval time is plucked in powdery mildew morbidity tobacco leaf upper and lower surface spray
Apply 50,000,000,000 spore L of bacillus subtilis-1Liquid, leaf natural ventilation are toasted after drying.
Embodiment 2
Provided in this embodiment is method 2, and bacillus subtilis microbial agent is applied by following concentration and time: bacillus subtilis
Bacterium wettable powder is diluted to 50,000,000,000 spore L-1；It is sprayed after being stacked 4 hours after picking in powdery mildew morbidity tobacco leaf upper and lower surface
50,000,000,000 spore L of bacillus subtilis-1Liquid, leaf natural ventilation are toasted after drying.
Embodiment 3
Provided in this embodiment is method 3, and bacillus subtilis microbial agent is applied by following concentration and time: Ko subtilis bar
Bacterium wettable powder is diluted to 100,000,000,000 spore L-1Liquid；After fresh tobacco leaf is stacked 4 hours after picking on powdery mildew morbidity tobacco leaf
Lower two sides sprays 100,000,000,000 spore L of bacillus subtilis-1Liquid, leaf natural ventilation are toasted after drying.
Embodiment 4
Provided in this embodiment is method 4, and bacillus subtilis microbial agent is applied by following concentration and time: Ko subtilis bar
Bacterium aqua is diluted to 150,000,000,000 spore L-1Liquid；In powdery mildew morbidity tobacco leaf upper and lower surface after fresh tobacco leaf is stacked 8 hours after picking
Spray 150,000,000,000 spore L of bacillus subtilis-1Liquid, leaf natural ventilation are toasted after drying.
Embodiment 5
Provided in this embodiment is method 5, and bacillus subtilis microbial agent is applied by following concentration and time: Ko subtilis bar
Bacterium suspending agent is diluted to 200,000,000,000 spore L-1Liquid；After fresh tobacco leaf is stacked 12 hours after picking two above and below powdery mildew morbidity tobacco leaf
Face sprays 200,000,000,000 spore L of bacillus subtilis-1Liquid, leaf natural ventilation are toasted after drying.
Embodiment 6
Method 6 provided in this embodiment, bacillus subtilis microbial agent are applied by following concentration and time: Bacillus subtillis
250,000,000,000 spore L of suspending agent-1；Withered grass is sprayed in powdery mildew morbidity tobacco leaf upper and lower surface after fresh tobacco leaf is stacked 24 hours after picking
250,000,000,000 spore L of bacillus-1Liquid, leaf natural ventilation are toasted after drying.
Embodiment 7
Method 7 provided in this embodiment, bacillus subtilis microbial agent are applied by following concentration and time: Bacillus subtillis
300,000,000,000 spore L of aqua-1；Withered grass bud is sprayed in powdery mildew morbidity tobacco leaf upper and lower surface after fresh tobacco leaf is stacked 36 hours after picking
300,000,000,000 spore L of spore bacillus-1Liquid, leaf natural ventilation are toasted after drying.
The following are bacillus subtilises of the present invention and different agents in Tobacco Leaf Curing, and melon infected with powdery mildew fungus is anti-
Imitate comparative experiments.
Experimental field it is arranged in the white salt well village in Zunyi City, Guizhou Province Tongzi county (height above sea level 1200m, 28 ° of north latitude, 106 ° of east longitude), ground
Block is more smooth, tobacco planting density: line-spacing 110cm, spacing in the rows 70cm or so, and average be 900 plants or so per acre.Barn is standard
Leaf flush mounting type barn is dissipated, barn is brick wall stationary structure, and barn is whole to be made of cigarette-filling house and hot wind generation room.Tobacco cultivation
Kind are as follows: Nan Jiang tri-.Material to be tested: physics plant protection liquid (hundred million good rural area environmental science and technology Co., Ltd of Dalian)；Hundred million spore of l000
Sub- g-1Bacillus subtillis (Jiangxi Shun Quan Biotechnology Co., Ltd)；50% thiophanate-methyl (Jiangsu sword board agrochemical share
Co., Ltd).
It is diluted (table 1) according to bacillus subtilis bacterium solution concentration described in embodiment 3, bacillus subtilis and different medicines
Agent Powdery Mildew Secondary Infection preventive effect comparative experiments in Tobacco Leaf Curing, experimental result is shown in Fig. 1 and Fig. 2.
1 different disposal pharmacy application ratio of table
Test result show after tobacco leaf sprays bacillus subtilis disease index compared to all decreasing before roasting, prevention and treatment effect
Fruit reaches 36.59%.This is the experiment proves that tobacco leaf powdery mildew can infect again in baking process；Tobacco leaf is sprayed before baking withered
Careless bacillus is not only avoided that loss caused by powdery mildew infects, and can mitigate powdery mildew occurring degree.
Finally, it is stated that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (1)
1. the method that powdery mildew infects in the prevention and treatment tobacco flue-curing of foliage-spray microbial bacterial agent, which is characterized in that including walking as follows
It is rapid: to spray bacillus subtilis microbial agent in powdery mildew morbidity tobacco leaf upper and lower surface after fresh tobacco leaf is stacked 4 hours after tobacco leaf picking, institute
Stating bacillus subtilis microbial agent spraying concentration is 100,000,000,000 spore L-1, toasted after blade dries, the maximum temperature of tobacco flue-curing
It is 68 DEG C.
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| CN105733991B (en) * | 2016-03-30 | 2019-08-02 | 贵州大学 | A kind of Bacillus amyloliquefaciens strain and its application in prevention and treatment Powdery Mildew in Tobacco |
| CN106942276A (en) * | 2017-03-16 | 2017-07-14 | 河北省农林科学院植物保护研究所 | Bacillus subtilis BAB 1 prevents and treats the application of cucurbits powdery mildew |
| CN110250209B (en) * | 2019-06-19 | 2021-05-28 | 云南省微生物发酵工程研究中心有限公司 | Microbial agent with function of preventing and treating mildew and rot of tobacco as well as preparation method and application of microbial agent |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250814A (en) * | 2011-07-20 | 2011-11-23 | 范成明 | Bacillus subtilis and use thereof in bio-control preparation for preventing and controlling powdery mildew |
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| KR100914226B1 (en) * | 2009-02-17 | 2009-08-26 | 주식회사 세실 | Bacillus subtilis sm010b strain and method for controlling plant pathogens using the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102250814A (en) * | 2011-07-20 | 2011-11-23 | 范成明 | Bacillus subtilis and use thereof in bio-control preparation for preventing and controlling powdery mildew |
Non-Patent Citations (2)
| Title |
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| 温度、超声、nisin协同脉冲磁场杀灭枯草芽孢杆菌的研究;钱静亚 等;《现代食品科技》;20131231;第29卷(第12期);第2970-2974页 |
| 烟叶烘烤过程中白粉病菌的侵染情况;卢志伟 等;《贵州农业科学》;20150228;第43卷(第2期);第50-52页 |
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