KR100914226B1 - Bacillus subtilis sm010b strain and method for controlling plant pathogens using the same - Google Patents

Abstract

A novel Bacillus subtilis strain having the effect against plant pathogen is provided to prevent from the plant pathogen containing bacterium and fucs without harmfulness. A Bacillus subtilis of deposit number KCTC11439 is isolated form compost using Fusarium oxyporum as a selection marker; A composition for preventing from plant pathogen comprises Bacillus subtilis, suspension of the strain, culture medium, and concentrate of the culture mediu.

Description

Bacillus subtilis SM010B strain and method for controlling plant pathogens using the same

The present invention relates to a novel Bacillus subtilis strain having a control effect against phytopathogens, a composition for controlling phytopathogens comprising the same, and a method for controlling phytopathogens using the same.

Environmentally friendly technologies are being promoted to control pests of plants. Biopesticides have not been produced on a large scale due to economic problems and problems in the preparation and use of preparations. Recently, research on biological control of plant diseases has been encouraging, and as a result, the development of microbial pesticides using antagonistic fungi and bacteria has been developed in foreign countries (Cook and Baker, 1983; Kim et al., 1990; Dandurand et al. 2000; Jack and Robert, 1998; Jack and Robert, 2001). For example, Coniothyrium minitans (Whipps and Gerlagh, 1992), Serratia plymuthica (Merav et al., 2003), Ulocladium atrum (Li et al., 2003), Pseudomonas fluorescens (Pedras et al., 2003), Bacillus megaterium N4 (Refill) Et al., 2002), and Nostoc strain ATCC 53789 (Biondi et al., 2004).

At present, the global biopesticide market is small at about 700 billion won, but it continues to grow at a rapid rate of 12% annually, especially in the microbial pesticide sector. Steve, L, 1999; Knight et al., 1997).

Therefore, there is a need for the development of biological control agents that are environmentally friendly, harmless to humans, and have a control activity against various phytopathogens.

Thus, one example of the present invention provides a novel Bacillus subtilis strain having a control effect against phytopathogens.

In another embodiment, the present invention provides a phytopathogen control agent comprising the Bacillus subtilis strain as an active ingredient.

In another example, the present invention provides a method for controlling phytopathogenic bacteria comprising applying the Bacillus subtilis strain to a plant.

The present invention relates to a novel Bacillus subtilis strain having a control effect against phytopathogens, a composition for controlling phytopathogens comprising the same, and a method for controlling phytopathogens using the same.

The present inventors have successfully isolated and identified Bacillus subtilis SM010B strain (Accession No .: KCTC11439BP) having excellent antimicrobial activity against phytopathogens and completed the present invention.

Accordingly, one example of the present invention provides a Bacillus subtilis SM010B strain of Accession No. KCTC11439BP having antibacterial activity against phytopathogens.

In another example, the invention provides a bacterium of Bacillus subtilis SM010B strain of Accession No. KCTC11439BP, a suspension of the strain, a culture of the strain, a concentrate of the culture, and a dried product of the culture. It provides a composition for controlling phytopathogenic bacteria containing at least one selected as an active ingredient.

In another embodiment, the present invention is one or more selected from the group consisting of Bacillus subtilis SM010B strain of Accession No. KCTC11439BP, the culture of the strain, the concentrate of the culture, and the dried product of the culture. It provides a phytopathogen control method comprising the step of applying to the plant.

Bacillus subtilis SM010B strain according to the present invention has a control effect against a variety of plant pathogens, including bacteria and fungi, and as shown in the following examples of spot pattern bacteria ( Alternaria panax ), black pattern bacteria ( Alternaria brassicicola ), leaf spot fungus ( Bipolaris sorokiniana ), gray fungus ( Botrytis cinerea ), anthrax ( Colletotrichum acutatum ), wilting fungus ( Fusarium oxysporum ), red fungus ( Fusarium graminearum ), late blight ( Phytophthora ) Pyricularia grisea (Magnaporthe grisea ), Rhizoctonia solani , Sclerotinia sclerotiorum , and half rot fungus ( Verticillium) dahliae ), red rust fungus ( Puccinia) recondita ), Barley powdery mildew ( Blumeria) graminis f. sp. hordei ), Sphaerotheca fusca ) and the like has a control effect on at least one selected from the group consisting of. Among them, the control activities against Alternaria brassicicola , Colletotrichum acutatum , Phytophthora nicotianae , Verticillium dahliae , and Sphaerotheca fusca according to the present invention Bacillus subtilis Bacillus subtilis Bacillus subtilis strains, which have been shown to have high homology with the strain, have no known activity.

The cell is a bacterium of Bacillus subtilis SM010B strain means both the body and spores of the bacteria itself, the suspension means the state in which the cells are suspended in distilled water, saline, buffer, etc. The culture includes both cell-containing and cell-free cultures, and the concentrates and dried products are concentrated or dried according to methods commonly used in the art to which the present invention belongs.

Plants to which the phytopathogen control composition and / or phytopathogen control method according to the present invention are applicable are not particularly limited, such as pepper, paprika, tomato, strawberry, melon, melon, cucumber, potato, rice, wheat, barley, ginseng It is applicable to at least one plant selected from the group consisting of, but is not limited thereto. The applied concentration is preferably applied in an amount of 1 × ^{10 6} CFU / ml to 1 × ^{10 9} CFU / ml, but not limited to maximizing control effects, minimizing side effects, and economics.

By using the Bacillus subtilis SM010B strain according to the present invention for the control of phytopathogens, it is possible to control more phytopathogens while being environmentally friendly and harmless to human beings.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example 1 Isolation of Strains

20 g of compost sample was added to a Erlenmeyer flask containing 100 ml of sterile distilled water and stirred for 30 minutes to prepare a sample solution. After diluting the sample solution to 10 ⁵ ~ 10 ⁶ , take 200 ㎕ of each dilution and spread it on Tryptic soy agar and incubate at 30 ° C. When bacterial colonies appear, nutrient agar (Nutrient agar) Passage was used to isolate single colonies. To the separated single colonies with a potato agar medium (Potato Dextrose Agar) bacterial wilt (Fusarium oxysporum) in the culture were selected by colonies that have inhibitory activity against Fusarium oxysporum named SM010B. The strain SM010B was deposited on December 5, 2008 at the Korea Institute of Biotechnology and Biotechnology Center (KCTC), Daejeon, and was given the accession number KCTC11439BP.

Example 2: Antimicrobial Activity Assay Through Substitution of Phytopathogen and SM010B Strains

In order to confirm the antimicrobial activity of the SM010B strain isolated in Example 1, the strain was used by culturing at 28 ° C. for 3 days using nutritious agar medium (Nutrient Agar). A mycelial piece of 5 mm diameter was removed from the mycelial path of the precultured pathogen, and inoculated onto PDA medium. A 6 mm diameter paper disc was placed on the opposite side or the center of the culture medium. After culturing for 7 days at the incubation temperature of each pathogen, the antimicrobial activity of the isolated strain was examined by measuring the diameter of the inhibition zone by the isolated strain.

The results obtained above are shown in Table 1 below.

[Table 1] Antimicrobial activity of phytopathogenic bacteria of SM010B strain

Target pathogen Antimicrobial activity Incubation temperature Alternaria panax Spot ++ 25 ℃ Alternaria brassicicola Germ ++ 25 ℃ Bipolaris sorokiniana Leaf spot + 25 ℃ Botrytis cinerea Gray mold ++ 25 ℃ Colletotrichum acutatum Anthrax ++ 25 ℃ Fusarium oxysporum Wither +++ 25 ℃ Fusarium graminearum Red fungal germs +++ 25 ℃ Phytophthora nicotianae Late blight ++ 25 ℃ Pyricularia grisea (Magnaporthe grisea) Blight germ ++ 25 ℃ Rhizoctonia solani Mozolox bacillus + 25 ℃ Sclerotinia sclerotiorum Fungi + 25 ℃ Verticillium dahliae Half withered germ + 25 ℃

+++: Low support 20 mm or more in diameter.

++: Low support 10 mm or more in diameter and less than 20 mm.

+: Low support diameter less than 10 mm.

1 to 3 are micrographs showing that the growth of phytopathogens is inhibited by the SM010B strain when incubating the SM010B strain and various plant pathogens. Figure 1 shows the antimicrobial activity of the SM010B strain against Botrytis cinerea , it can be seen that the growth of gray fungus mycelium inhibited by the active material produced by SM010B (see red circle). Figure 2 shows the antimicrobial activity of the SM010B strain against anthrax (colletotrichum acutatum ) can be confirmed that the growth of anthrax mycelia by the active material produced by SM010B is inhibited (see red circle). Figure 3 shows the antimicrobial activity of the SM010B strain against Magnaporthe grisea (Mnanaporthe grisea ) it can be confirmed that the growth of the bacillus mycelia by the active material produced by SM010B.

Example 3: Identification of Isolated Strains

In order to investigate the morphological culture characteristics of the isolated strain SM010B, a solid nutrition medium such as potato agar (Potato agar), corn-molasses agar medium (Maize molasses agar), meat peptone agar (Nutrient Media) ) Was incubated at 28 ± 2 ° C. for 3 to 5 days, and then bacteria were observed under a microscope, and 16S rDNA sequencing was performed.

To amplify the fragments required for 16S rDNA sequencing, genomic DNA and 27F primer (5'- AGAGTTTGATYMTGGCTCAG-3 '(Y = C or T, M = A or C), SEQ ID NO: 2) of the SM010B strain, 1492R PCR reactions were performed using primers (5'- TACGGYTACCTTGTTACGACT-3 '(Y = C or T), SEQ ID NO: 3). PCR reaction was performed using GeneAmp ^™ PCR System 9700 (Applied Biosystem), 1 cycle at 94 ° C for 3 minutes, 30 seconds at 94 ° C, 30 seconds at 50 ° C, and 5 minutes at 72 ° C for a total of 30 cycles. Then, 1 cycle was performed for 10 minutes at 72 ℃. PCR results were confirmed by electrophoresis on 1% agarose gel and 16S rDNA fragments were recovered. The recovered fragment was recovered ^{ABI PRISM TM Bigdye TM terminator cycle sequencing} Ready reaction kit V.3.1 (Applied Biosystem) and using the base sequence after performing the analysis of the reaction, a fluorescent label a fragment using a Montage remove dye kit (Millipore) It was. The recovered fragment was developed using an ABI 3730XL capillary DNA sequencer (Applied Biosystem), and the base sequence was determined from the obtained result.

As a result of morphological culture characterization, SM010B strain is rod shape, 0.7 ~ 0.8 ㎛ in width, 2 ~ 3 ㎛ in length, is Gram-positive bacteria, forms only one elliptical spore in cell, Flagella was observed to be peritrichous flagella.

In all solid nutrient media, small white colonies of amorphous form were identified at the beginning of strain growth. After 48 hours of incubation in potato agar medium, the edges grow irregularly and grow into flat, white, unpolished colonies that are slightly thick and beige. Corn molasses agar medium was observed to grow in irregularly shaped edges and beige colonies with small wrinkles in the middle of the colonies. After a week, the colony became slightly darker. Glucose-containing meat peptone agar medium showed irregular edges, no gloss on the surface, and a center of colony growing into flat white colonies. Catalase (catalase) activity and oxidase (oxidase) activity, and starch (Starch) and casein (casein) is hydrolyzed and did not grow under anaerobic conditions. Growth temperature is up to 45-55 ° C, lowest is 5-10 ° C. Like most bacteria, it uses various nitrogen sources (peptone, casein, amino acids, proteins and their hydrolysates, yeast hydrolysates, etc.) It appeared to grow well. Glucose, sucrose and starch are used as nutrients. Acids are produced when glucose, arabinose, xylose and mannitol are used. It has been observed to reduce nitrate to nitrite.

The 16S rDNA sequence of the SM010B strain obtained as a result of 16S rDNA sequencing was shown in FIG. 4 and SEQ ID NO: 1.

The base sequence of 16S rDNA of the obtained SM010B strain showed 99.9% homology with Bacillus subtilis compared to the microorganism registered in NCBI GenBank, and confirmed that the closest, the isolated strain Bacillus subtilis ( Bacillus subtilis ) was named SM010B.

Example 4 Control Effect Test of Plant Disease of Bacillus Subtilis SM010B

In order to spread the Bacillus subtilis SM010B strain on the crop, the living microorganism cells and its culture solution, surfactant, acetylene carbon, yeast extract, etc. are mixed, concentrated and dried to form a powder. Prepared. At this time, the concentration of the microorganism was prepared to include 10 ¹⁰ CFU / g or more.

4.1. Control Effects of Bacillus Subtilis SM010B Against Red Rust

Since Puccinia recondita , a red rust pathogen, is a parasitic bacterium, urediniospores formed in wheat seedlings were used as inoculum while passing directly to plants in the laboratory. In order to investigate the efficacy of the strain, five seed seeds (variety: silver onion) were sown in disposable pots (4.5cm in diameter), treated with 1 leaf seedling seedlings grown in a greenhouse for 8 days, and then treated with the inoculator (spore 0.22g / L) was sprayed. Inoculated wheat seedlings were incubated at 20 ° C. for 1 day and then transferred to a constant temperature and humidity room at 20 ° C. with a relative humidity of 70%. The disease area ratio obtained from the disease survey was calculated according to the following formula to calculate the control value.

Control value (%) = (Length of area of 1-treated area / Lesion of area of non-treated area) × 100

The control value obtained as described above is shown in Table 2 below.

TABLE 2

SM010B Dilution Drainage 250-fold dilution 500-fold dilution Control is (%) 77 70

As shown in Table 2, the Bacillus subtilis SM010B strain was confirmed to have an excellent control effect against red rust. Figure 5 is a photograph showing the control activity against red rust disease of the Bacillus subtilis SM010B strain, there are many symptoms of red rust disease in the untreated group, while the symptom was significantly reduced in the experimental group treated with the strain.

4.2. Control Effect of Bacillus subtilis SM010B on Barley Powder

Blumeria graminis f. sp. Since hordei is a biomolecular bacterium, spores formed in barley were used as inoculum during subculture in barley seedlings. Pharmacokinetic investigation was conducted by spraying 5 seedlings of barley seeds (variety: copper barley) into disposable pots (4.5 cm in diameter) and spraying the medicinal plants for 1 day seedlings grown in greenhouses for 8 days, air-dried in greenhouses, and powdered barley powder on treated barley. The spores were shaken off and inoculated. Inoculated barley seedlings were placed in a constant temperature and humidity room at 20-23 ° C. and a relative humidity of 50% for 7 days, and the lesion area ratio was examined. The disease area ratio obtained from the disease survey was calculated according to the following formula to calculate the control value.

Control value (%) = (Length of area of 1-treated area / Lesion of area of non-treated area) × 100

The control value obtained as described above is shown in Table 3 below.

TABLE 3

SM010B Dilution Drainage 250-fold dilution Control is (%) 76

As shown in the table, the Bacillus subtilis SM010B strain was confirmed to have an excellent control effect against barley powdery mildew. Figure 6 shows the control activity against barley powdery mildew of the Bacillus subtilis SM010B strain, while the symptoms of powdery mildew in the non-treated group, it can be seen that significantly reduced in the experimental group treated with the strain.

4.3 Control Effects of Bacillus Subtilis SM010B Strain on Anthrax

The control activity of Bacillus subtilis SM010B against anthrax was investigated using red pepper fruit. In order to investigate the control effect using red pepper fruits, peppers infected with anthrax germ were collected from red pepper plantation, pure anthrax ( colletotrichum acutatum ) was isolated, and transplanted into potato agar medium (PDA medium) in a cancerous condition at 25 ° C. After incubation for 10 days, 20 ml of distilled water was poured to harvest conidia formed on the surface of the medium. The suspension of conidia was filtered through four layers of gauze to remove the mycelia and debris from the medium, and the number of conidia in the suspension was adjusted to 1 × 110 ⁶ / ml. The control fungicide (anthrachol, Dongbu HiTek) and Bacillus subtilis SM010B used in the experiment were sprayed on red pepper (variety: green mine) and air-dried at room temperature for 1 day. The spore suspension of the anthrax was prepared by spraying the surface of red pepper, and wet-treated in the dark at 30 ° C. for 5 days, and then removed from the wet chamber and stored at the same temperature for 5 days to induce the onset. After 10 days of inoculation, the incidence of each fruit was examined to calculate the control value.

Incidence (%) = [∑ (Number of Incidents X Coefficient) ÷ 5N] X 100 (%)

           Counting criteria

           0: No onset (0%), 1: Less than 1% of onset

           2: onset 1 ~ 10% 3: onset 10 ~ 25%

           4: onset 25 ~ 50% 5: onset 50% or more

           N: Survey Number

Control value (%) = (degree of incidence / treatment-free of 1-treatment tool) * 100

The control value obtained as described above is shown in Table 4 below.

TABLE 4

 Dilution factor Bacillus subtilis SM010B Anthracol No treatment 500-fold dilution 1000-fold dilution 500-fold dilution Incidence (%) 3.7 15.6 8.1 40.0 Control price (%) 90.8 61.0 79.8 -

As shown in Table 4, the Bacillus subtilis SM010B strain exhibited better activity of pepper anthracnose control than anthracol, a drug commonly used as a conventional control agent, and thereby significantly controlling the anthrax of Bacillus subtilis SM010B strain. The effect was confirmed.

4.4. Control Effect of Bacillus Subtilis SM010B Against Powdery mildew

Since Sphaerotheca fusca , a powdery mildew pathogen, is a lipophilic bacterium, it is possible to plant cucumber seeds (cultivar: Houseback teapot) on horticultural soils and cultivate them in a greenhouse for about 4 weeks to ensure sufficient powdery mildew on one and two leaves of five to six leafy cucumbers. It confirmed that it occurred. Cucumber seedlings with powdery mildew were treated primarily with Bacillus subtilis SM010B and then treated with a second time after one week. One week after the second treatment, the lesion area ratio of each leaf from the 3rd to 11th leaves of the cucumber was examined. The disease area ratio obtained from the disease survey was calculated according to the following formula to calculate the control value.

Incidence (%) = [∑ (Number of Incidents X Coefficient) ÷ 4N] X 100 (%)

           Counting criteria

           0: No disease (0%), 1: Less than 5% of lesion area

           2: Area area of disease 5 ~ 20% 3: Area area of disease 20 ~ 40%

           4: lesion area area of 40% or more N: irradiation number

Control value (%) = (degree of incidence / treatment-free of 1-treatment tool) * 100

The control value obtained as described above is shown in Table 5 below.

TABLE 5

Treatment Sample Dilution factor Incidence (%) Control price (%) Bacillus subtilis SM010B 250-fold dilution 27.8 57.4 500-fold dilution 42.5 34.9 EcoJet (Dongbu Hitech) (Control Microorganism) 250-fold dilution 46.0 29.6 500-fold dilution 52.8 19.1 No treatment 65.3 -

As confirmed in Table 5, the Bacillus subtilis SM010B strain exhibited better activity for controlling cucumber powdery mildew than Ecojet, a microbial pesticide commonly used as a conventional control agent, and thus, to the powdery mildew of Bacillus subtilis SM010B strain. Significant control effects were identified.

As mentioned above, the Bacillus subtilis SM010B strain showed excellent control against red rust, barley powdery mildew, powdery mildew, anthracnose, gray fungus, wilted rot, late blight, heat-resistant bacillus, mozzarella, fungal nucleus, and spot bacteria. Since the antagonism of the plant can be useful for controlling plant diseases.

Figure 1 is a photograph showing the antimicrobial activity of the SM010B strain against the gray mold ( Botrytis cinerea ).

Figure 2 is a photograph showing the antimicrobial activity of the SM010B strain against anthrax (colletotrichum acutatum ).

Figure 3 is a photograph showing the antimicrobial activity of the SM010B strain against rice blast bacteria ( Magnaporthe grisea ).

Figure 4 shows the 16S rDNA sequence of the Bacillus subtilis SM010B strain.

5 is a photograph showing the control activity against wheat rust disease of Bacillus subtilis SM010B strain.

Figure 6 is a photograph showing the control activity against barley powdery mildew of Bacillus subtilis SM010B strain.

<110> SESIL Corporation <120> Bacillus subtilis SM010B strain and method of controlling plant          pathogens using the same <160> 3 <170> KopatentIn 1.71 <210> 1 <211> 1426 <212> DNA <213> Artificial Sequence <220> <223> nucleotide sequence of 16S rDNA of SM010B strain <400> 1 atacatgcag tcgagcggac agatgggagc ttgctccctg atgttagcgg cggacgggtg 60 agtaacacgt gggtaacctg cctgtaagac tgggataact ccgggaaacc ggggctaata 120 ccggatggtt gtttgaaccg catggttcaa acataaaagg tggcttcggc taccacttac 180 agatggaccc gcggcgcatt agctagttgg tgaggtaacg gctcaccaag gcaacgatgc 240 gtagccgacc tgagagggtg atcggccaca ctgggactga gacacggccc agactcctac 300 gggaggcagc agtagggaat cttccgcaat ggacgaaagt ctgacggagc aacgccgcgt 360 gagtgatgaa ggttttcgga tcgtaaagct ctgttgttag ggaagaacaa gtaccgttcg 420 aatagggcgg taccttgacg gtacctaacc agaaagccac ggctaactac gtgccagcag 480 ccgcggtaat acgtaggtgg caagcgttgt ccggaattat tgggcgtaaa gggctcgcag 540 gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc attggaaact 600 ggggaacttg agtgcagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga 660 gatgtggagg aacaccagtg gcgaaggcga ctctctggtc tgtaactgac gctgaggagc 720 gaaagcgtgg ggagcgaaca ggattagata ccctggtagt ccacgccgta aacgatgagt 780 gctaagtgtt agggggtttc cgccccttag tgctgcagct aacgcattaa gcactccgcc 840 tggggagtac ggtcgcaaga ctgaaactca aaggaattga cgggggcccg cacaagcggt 900 ggagcatgtg gtttaattcg aagcaacgcg aagaacctta ccaggtcttg acatcctctg 960 acaatcctag agataggacg tccccttcgg gggcagagtg acaggtggtg catggttgtc 1020 gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc cttgatctta 1080 gttgccagca ttcagttggg cactctaagg tgactgccgg tgacaaaccg gaggaaggtg 1140 gggatgacgt caaatcatca tgccccttat gacctgggct acacacgtgc tacaatggac 1200 agaacaaagg gcagcgaaac cgcgaggtta agccaatccc acaaatctgt tctcagttcg 1260 gatcgcagtc tgcaactcga ctgcgtgaag ctggaatcgc tagtaatcgc ggatcagcat 1320 gccgcggtga atacgttccc gggccttgta cacaccgccc gtcacaccac gagagtttgt 1380 aacacccgaa gtcggtgagg taacctttta ggagccagcc gccgaa 1426 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 27F primer <400> 2 agagtttgat ymtggctcag 20 <210> 3 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> 1492R primer <400> 3 tacggytacc ttgttacgac t 21  

Claims (7)

Bacillus subtilis SM010B strain of Accession No. KCTC11439BP. At least one selected from the group consisting of Bacillus subtilis SM010B strain of Accession No. KCTC11439BP, suspension of said strain, culture of said strain, concentrate of said culture, and dried product of said culture. Containing, Alternaria panax , Alternaria brassicicola , Bipolaris sorokiniana , Bacterial fungus ( Botrytis cinerea ), Anthracnose ( Calletotrichum acutatum ), Fusarium oxysporum , Red fungus ( Fusarium) graminearum), Station pathogens (Phytophthora nicotianae), rice blast fungus (Pyricularia grisea, Magnaporthe grisea), damping-off fungi (Rhizoctonia solani), gyunhaekbyeong fungus (Sclerotinia sclerotiorum), half wilt fungi (Verticillium dahliae), red rust (Puccinia recondita), wheat It has a control activity against at least one selected from the group consisting of Blumeria graminis f. Sp.hordei and Cucumber Sphaerotheca fusca . Composition for controlling plant pathogens. delete delete Applying to the plant at least one selected from the group consisting of Bacillus subtilis SM010B strain of Accession No. KCTC11439BP, a culture of said strain, a concentrate of said culture, and a dried product of said culture. doing, Alternaria panax , Alternaria brassicicola , Bipolaris sorokiniana , Bacterial fungus ( Botrytis cinerea ), Anthracnose ( Calletotrichum acutatum ), Fusarium oxysporum , Red fungus ( Fusarium) graminearum), Station pathogens (Phytophthora nicotianae), rice blast fungus (Pyricularia grisea, Magnaporthe grisea), damping-off fungi (Rhizoctonia solani), gyunhaekbyeong fungus (Sclerotinia sclerotiorum), half wilt fungi (Verticillium dahliae), red rust (Puccinia recondita), wheat A method for controlling phytopathogens against at least one species selected from the group consisting of powdery mildew ( Blumeria graminis f. Sp. Hordei ), and powdery mildew ( Sphaerotheca fusca ). The method of claim 5, wherein The plant is one or more selected from the group consisting of pepper, paprika, tomato, strawberry, melon, melon, cucumber, potato, rice, wheat, barley, and ginseng, Phytopathogen control method. delete

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