KR101107331B1 - Novel Streptomyces argenteolus strain having activity aganist plant pathogens - Google Patents

Abstract

The present invention includes a novel Streptomyces argentherus strain (KCTC 11524BP) having antibacterial activity against plant pathogens, a pesticide preparation for plant disease control containing the strain as an active ingredient, and culturing the strain. The present invention relates to a method for preparing a pesticide preparation for controlling plant diseases, in which the novel strain has an excellent control effect against red pepper disease, anthrax and root rot.

Streptomyces Argenteorus, Pepper Disease, Plant Disease Control, Microbial Pesticides

Description

Novel Streptomyces argenteolus strain having activity aganist plant pathogens

The present invention includes a novel Streptomyces argentherus strain (KCTC 11524BP) having antibacterial activity against plant pathogens, a pesticide preparation for plant disease control containing the strain as an active ingredient, and culturing the strain. It relates to a method for producing a pesticide preparation for plant disease control.

In order to solve the problem of food security caused by global population growth, pesticide and weed control methods using chemical pesticides have been recognized as an indispensable factor in increasing agricultural productivity. However, the abuse of chemical pesticides has caused side effects such as pesticide poisoning, groundwater contamination, soil pollution, residues on agricultural products, and the appearance of resistant individuals to various pests.

Thus, efforts were made to protect the global environment since the Rio Declaration of the UN Conference on Environmental Development, and the Green Round Convention was in full swing, limiting the abuse of chemical pesticides and using them to replace biological pesticides. This is attracting attention. Therefore, it is urgent to select antagonistic microorganisms capable of effectively suppressing plant pathogens while minimizing environmental pollution, and to develop a pollution-free pesticide (biocide) for plant disease control using the same.

In general, crop diseases caused by plant pathogens are a very limiting factor in the stable production of crops along with pests and weeds. Methods for controlling such pathogens include physical methods to improve the cultivation environment, chemical methods using fungicides and microbiological methods using microorganisms. However, physical control alone cannot be expected to have a great effect, and chemical control, as mentioned above, has greatly reduced the control effect due to the problem of environmental pollution caused by chemical pesticides and the emergence of pesticide-resistant pathogens. Therefore, researches on biological control by antagonistic microorganisms have been actively conducted in recent years.

The principle of biological control by antagonistic microorganisms is to reduce the density or pathogenesis of pathogens through antibiotics produced by antibiotics, parasitic or phagocytosis by cell wall degrading enzymes, and nutrient competition or niche competition. The main mechanisms of action include cross-protection and induction of host resistance using non-pathogenic strains or weakly-pathogenic strains. For example, the inhibitory action of fungal diseases caused by antibiotics or volatile substances, toxins, and special inhibitors secreted by the genus Trichoderma and gliocladium is known. In addition, antagonism by parasitic or phagocytosis has been actively studied in recent years, and the dissolution of pathogens by cell wall degrading enzymes of fungi secreted by antagonistic bacteria is known as the main mechanism of action.

Typical microbial agent developed and commercialized in a foreign country is currently tricot der Do not annuum (Trichoderma harzianum) and tricot der Matthew Polis Forum (T. polysporum) made a BINAB T (1988, ER Butts International ) to inhibit Wood rot fungi, writing GlioGard (1990, WR Grace Co.), a bottle control agent for greenhouse flower seedlings using Gliocladium virens , Fstop (1991, Kodak), Trichodex (1997, Israel) Developed by KODIAK (1990, Gustafson) and Pseudomonas fluorescens using seed antagonist Bacillus subtilis) to control cotton lipotonia and Pythium sp. DAGGER (1990, Ecogen Inc.), Pseudomonas Sefar Asia (P. cepacia) for BLUE CIRCLE the seed treatment of multiple crops using (1990, Stine Microbial Products), and antagonistic actinomycetes host Sat My process and the like so Seo irregularities disk (Streptomyces griseoviridis) the Fusarium (Fusarium) wilt, damping-off control agent MYCOSTOP (1985, Kemira Oy Biocontrol of Finland) using.

On the other hand, in Korea, research on biological control of ginseng root rot has been conducted since the early 1970s, and the research on biological control of red pepper disease, cucumber wilting disease, sesame seedling disease, and ash fungus has been conducted. It is true. Bacteria among the microorganisms for fungicides patented as microbial pesticides include Bacillus sp. BS555, KCTC 8821P, Bacillus poly Pastor Bacillus polymoxa B36, FERM BP-6068) , Pseudomonas Oreo Pacific Enschede (Pseudomonas aureofaciens B5, FERM BP-6067) , Enterobacter claw dehydratase (Enterobacter cloacae B51, FERM BP-6069) and Bom1 (KFCC 10992) and Bom2 (KFCC 10993) in Streptomyces, an actinomycetes that secrete antifungal substances. In addition, as a microorganism having an antagonistic ability against pathogenic fungi Bacillus subtilis bar amylolytic faciens ( Bacillus subtilis var. amyloliquefaciens KL114, KCTC 8913P), Bacillus amyl Lori query Patience (Bacillus amyloliquefaciens KTGB0202 KCTC 10564BP), Alcaligenes faecalis KL1179, KCTC 8914P), rugi Pseudomonas Ah industrial (Pseudomonas aeruginosa KL1121, KCTC 8915P) , Pseudomonas brother is thiazol car (Pseudomonas aurantiaca KL1326, KCTC 8916P), Bacillus subtilis and Pseudomonas fluorescences, and Pseudomonas fluorescence bios 1 (Biovar 1), which have antagonistic ability against actinomycetes Streptomyces genus, and fungi Delmar hazinium, Penicillium sp., Aspergillus sp., Acremonium strithium BCP, Acremonium strictum, KCTC 0639BP), etc. under study.

Among them, Korean Patent Registration No. 10-0757298 relates to lysobacter enzymogenes and strain of chromobacterium having plant disease control activity using chitin decomposition ability, but the production ability of antagonist microorganism and chitinase At the same time, there is a disadvantage in that the production of a medium that can be exerted at the same time is not easy, and the strain is different from the Streptomyces argenteo strain of the present invention.

As described above, various microbial pesticides having antagonistic action against phytopathogenic bacteria have been reported at home and abroad, but microorganisms having excellent antagonistic action that can effectively control various pathogenic bacteria causing disease of various crops have not been reported. . Therefore, in the present invention, the new antagonistic microorganism Streptomyces argentherus, which has not been reported so far, has been searched from soils all over the country, and the result of large-scale cultivation of efficacy test results shows excellent antibacterial activity against various pathogens. As well as showing particularly excellent antagonistic activity in pepper blight, anthrax and root rot disease and completed the present invention.

One object of the present invention is Streptomyces ( Streptomyces) having an antimicrobial activity against plant pathogens that cause pepper blight, anthrax and root rot argenteolus) .

Still another object of the present invention is to provide a pesticide preparation for plant disease control and a method of preparing the same, containing the cells, spores, culture medium or mixtures of the Streptomyces argentherus strain as an active ingredient.

Another object of the present invention relates to a method for controlling plant diseases using a pesticide preparation containing a Streptomyces argeneorus strain.

The present invention has antibacterial activity against plant pathogens Streptomyces Argen Theorus ( Streptomyces argenteolus ) strain, a pesticide preparation for plant disease control containing the strain as an active ingredient, and a method for producing a pesticide preparation for plant disease control comprising the step of culturing the strain.

In one embodiment, the present invention relates to a strain of Streptomyces sp. That has antimicrobial activity against plant pathogens, and more particularly to plants that cause plant pathogens, in particular, cayenne blight, anthrax and root rot. It relates to a novel Streptomyces argentherus strain having antimicrobial activity against pathogens.

As used herein, the term "streptomyces" is a representative genus of soil actinomycetes and produces antibiotics, and is a fungi belonging to the actinomycetes Streptomyces family and has a characteristic of forming a conidia in the mycelia. It's a group of people.

Streptomyces argenteorus strain of the present invention is preferably Streptomyces argenteolus subsp.toyonakensis . The novel Streptomyces argentheor toyonakensis subspecies is isolated from the soil of good parts, and has a strong antibacterial activity in common to Phytophthora cactorum and Colletotrichum acutatum . The microorganisms were selected, isolated and identified, and the present inventor named it Streptomyces argentherus rho-44, and deposited it on July 6, 2009 to the Korea Biotechnology Research Institute Gene Bank, and assigned accession number KCTC 11524BP. received.

Among many plant diseases, pepper blight, anthrax, and root rot are representative diseases that cause enormous damage in the harvest of crops. Cayenne pepper disease is a major disease of pepper that occurs in all parts of the roots, stems, leaves, and fruits throughout its growth period. Phytophthora cactorum , its pathogen, is very pathogenic. Invade a lot of damage. In addition, anthrax in plants is caused by a fungus called gnus Colletotrichum , the main pathogen of which is Colletotrichum acutatum . This fungus destroys leaves, stems, fruits, and flowers. It is one of the most serious diseases in eggplants and gourds and crops, such as pepper, which is indispensable in Korean food, and watermelon, which is a summer fruit. Root rot is a condition in which part or all of the roots of a plant are broken down or rotted. When the pathogens Rhizoctonia solani and Pythium ultimum are infected, the internal tissues of the roots are destroyed and black rots. In addition, the root's ability to absorb is reduced, causing leaves to fall and damage to the fruiting rate dropping significantly. Accordingly, the present invention provides a strain of Streptomyces argenteorus, which is a kind of biopesticide capable of exhibiting antimicrobial activity against all of the plant pathogens.

Here, the biopesticide may be defined as the product of the microorganisms and natural materials, natural enemies, etc. collected and collected in the natural environment to control pests and pathogenic microorganisms, weeds of crops.

The strain of the present invention is preferably resistant to the phytophthora japtorum, which is the causative agent of pepper blight, choletotricum akutat, which is the causative agent of anthrax, and the lysatonia solani, which is the causative agent of root rot, and fishium Ultimum. It has antimicrobial activity (FIG. 4).

In addition, the Streptomyces argentheor toyonakensis subspecies of the present invention, in addition to the above-mentioned plant pathogens, Fusarium solani and Cylidrocarpon dextans ( Cylidrocarpon ), which are causes of root rot. destructans ), Botrytis , the causative agent of gray mold cinerea ), Pythium , the causative agent of incoming and mozalococcal disease utimum ), Altenaria panax (Altenaria ), the causative agent of spots panax ), Sclerotinia sclerotiorum , which is the causative agent of mycobacterial disease, and Streptomyces scabies , which are the causative agent of potato beetle disease, but are not limited thereto. In particular, the strains of the present invention exhibit strong antimicrobial activity against Pisium Ultimum, Botrytis Ceria, Altenaria Panax and Fusarium Solani (Figs. 3, 5, 6 and 8). In addition, these pathogens are most often caused by complex infections that are expressed in correlation rather than alone.

Thus, since the strain of the present invention exhibits strong antagonism against pathogens causing various plant diseases, including pepper blight, anthrax and root rot, the strain is used as an environmentally friendly, non-polluting biopesticide. It can be useful for solving ecosystem destruction and toxicity problem.

As another aspect of the present invention, the present invention relates to a pesticide preparation for controlling plant diseases containing cells, spores, culture medium or mixtures of novel Streptomyces argentherus toyonakensis subspecies as an active ingredient. . More specifically, when the foliar spray of a culture medium containing the microorganism of the present invention on the foliar crops, the causative agent of pepper blight phytophthora ( Phytophthora) cactorum), the causative agent of anthrax Collet sat tree glutamicum Aqua tatum (Colletotrichum acutatum ), Rhizoctonia , a causative agent of root rot solani ) relates to a microbial pesticide preparation having a control effect of phytopathogenic microorganisms containing the effect of inhibiting growth.

The culture solution included in the pesticide preparation of the present invention is a supernatant from which the strain is removed from the strain culture solution, which can be obtained by centrifugation or filtration, and can be included in the pesticide preparation for controlling plant diseases in a liquid or solid state after drying. Such a pesticide preparation may be prepared using a carrier usable in a pesticide preparation in addition to the cells of Streptomyces argentheor toyonakensis subspecies, its culture, or its culture extract. The carrier may be any material having a small grain size so that the antagonist can be adsorbed, but is preferably a granular material having a porosity, and may be preferably selected from zeolite, elvan, vermiculite, sand, and activated carbon. Most preferred.

The pesticide preparation of the present invention may be prepared by a known method in the pharmaceutical field, and in addition to the carrier, it may be prepared and used in various formulations, including excipients and diluents, and the antifungal active bacteria may be well adhered to the plant leaves or It may also contain a coating flocculant that will degrade well in the soil. The pesticide preparation of the present invention may be formulated as a liquid or emulsion, typically diluted with water and dispersed in powder, or previously formulated with water, or formulated as a water-soluble hydrate, granule, oil suspension. Alternatively, it may be prepared in the form of a ready mix or tank mix with other agrochemically active compounds such as toxic agents or growth regulators.

Preferably, the active ingredient may be used in 0.001 to 90% of the culture solution extract with respect to the entire pesticide formulation, more preferably may be used by diluting 100 to 500 times the strain culture extract according to the present invention.

As another aspect of the present invention, the present invention provides a method for controlling plant diseases by using the pesticide preparation for plant diseases control. Preferably, the plant disease may be pepper blight, anthrax, or root rot. Pesticides prepared as described above is preferably used as a foliage treatment method on the plant's ground portion from 10 days after planting the crop or just before the onset.

In addition, the pesticide formulation may be used as a general pesticide formulation such as seed coating agents, microbial nutrients, soil improving agents, in addition to plant disease control agents, but is not limited thereto.

As another aspect of the present invention, the present invention relates to a method for producing a pesticide preparation for plant disease control, comprising the step of culturing Streptomyces argentherus toyonaksis subspecies strain in a medium. More specifically, the preparation method of the microbial agent of the present invention is a microorganism Streptomyces that is antagonistic against plant pathogens such as Phytoprosora chitorum, Colletotricum akutatum, Raiztonia sorani, fishium Ultimum and the like. Separating the Genteorus strain from the soil; Identifying the isolated antagonistic microorganism Streptomyces argentherus strain; Treating the spore suspension of the isolated Streptomyces argentherus strain in agar solid medium, inoculating fungal pathogens and measuring the antibacterial activity to assay the antagonism of the Streptomyces argentherus strain; And mass culturing of the Streptomyces argeneorus strain.

Separation of the strain may be carried out by purely separating the colonies produced after 2 to 4 days incubation in YM medium after diluting the soil by mixing the sterile water with dilution. The isolated strain may be subjected to 16S rDNA sequencing to identify morphological characteristics and phylogenetic location to identify. 16S rDNA sequence analysis for the strain of the present invention is shown in FIG. In addition, the step of assaying the antagonism of the strain is buried in a culture medium cultured strain of the present invention on a paper disk and put on a petri dish mixed with pathogens and cultured for 3 to 4 days at 25 ~ 30 ℃ well grown various pathogens Thus, the effect of the strain of the present invention on various pathogens can be investigated with a low support diameter. In addition, the mass culture step may be a liquid medium in which yeast extract 3 ~ 6 g / L, juicy extract 3 ~ 6 g / L, glucose 8 ~ 12 g / L is added, at 25 ~ 30 ℃ 120 ~ 180rpm It can be performed for 3 to 7 days under the condition.

In general, when culturing a large amount of bacteria or when the number of samples to be investigated is large, the growth of bacteria can be measured by measuring the absorbance of the culture solution. Here, the term "Optical Density (OD)" means that when a certain wavelength of light passes through a bacterial culture, the solution can absorb (or scatter) the light, and the amount of light absorbed is dependent on the cell concentration of the solution. It uses the principle of proportionality, and it means the value measured by using an extinction meter, and it is used because the unknown number of cells can be found only by measuring the absorbance. Also in the present invention, the growth curve of the strain in the culture solution is shown in Figure 9 by measuring the absorbance.

The pesticide preparation of the present invention prepared as described above may be sprayed on the crop plantation to measure the number of crops produced per kilogram to analyze the growth of the crop. In the case of the present invention, the results for red pepper are shown in FIG.

The novel Streptomyces argentherus strain of the present invention shows excellent antimicrobial activity and inhibitory effect against plant pathogens that cause pepper blight, anthrax, and root rot disease, and microbial pesticide preparations containing the strain have various plant diseases. It is very useful as an environmentally friendly, pollution-free microbial pesticide that shows excellent control effect against and prevents ecosystem destruction.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are intended to illustrate the present invention, but the scope of the present invention is not limited thereto.

Example  1: Isolation and Identification of Strains

1. Strain Isolation

In this experimental example, soil samples were collected from all over the country in order to isolate the microorganisms having antimicrobial activity, using the pepper bacterium Phytophthora japtorum and the pepper anthrax colletotricum akutatum as test strains. Sampling was carried out by selecting a depth of about 15 centimeters of the topsoil layer, and removing foreign matters such as weeds and fallen leaves. It was sealed in a 200 ml glass bottle and transported to a laboratory at 0 to 4 ° C. 1 g of the collected sample was mixed with 10 mL of sterile water, shaken at 28 ° C. for 30 minutes, diluted 10 ^4-6 times by soil dilution agar plate method, and 0.1 mL of the diluted solution was dispensed into YM medium to evenly spread the entire surface of the medium. Next, the colonies generated after incubation for 2 to 4 days in a 30 ℃ incubator were separated by pure water and transferred to a YM solid medium used at 4 ℃ storage. Test strains were each fungi phytopsora metolum, colletotricum akutum, Lyactonia solani was used, and one of the best strains showing strong antibacterial activity in common to all three strains was selected.

2. Strain Identification

Identification of the strains selected above was carried out as follows.

(1) Morphological Characterization of Strains

      The selected strains were shown in Table 1 by observing the culture and physiological characteristics of a single colony obtained by incubating in YM agar medium for 3 days at 30 ℃ culture in various media as shown in Table 1.

Culture Characterization of Streptomyces argentherus.  Analysis item characteristic    Spore color / hyphae color by badge type       ISP 2 badge Taupe / yellow       ISP 3 badge Taupe / yellow       ISP 4 badge Taupe / brown       ISP 5 badge Taupe / yellow    Spore chain morphology gyre    Spore Count / Spore Chain 10-20 pieces    Whether pigments are produced       ISP 2 badge U       ISP 3 badge radish       ISP 4 badge radish       ISP 5 badge U    Carbon use       Arabinose -       Fructose +       Glucose +       Mannitol +       Raffinose -       Sucrose +       Xylose +    Whether to grow when the following ingredients are added       5% (w / v) sodium chloride (NaCl) +       100 μg / ml thallium acetate -       1 μg / ml crystal violet +       20 μg / ml streptomycin -       10 μg / ml penicillin -    Lowest pH for growth 3.5

+: Possible,-: impossible

2. 16S rDNA Sequencing

In order to confirm the phylogenetic location of the selected strains, 446 bp sequences corresponding to more than 29% of the 16S rDNA sequences were analyzed. PCR amplification of 16S rDNA segment 5'-AGA GTT TGA TCM TGG CTC AG-3 'from the selected strains with a primer set, and then amplified product was purified and inserted into the TOPO TA cloning kit (Invitrogen). E. coli was transformed. After the plasmid was extracted from the transformed E. coli, the nucleotide sequence was analyzed using an Applied Biosystems model 373A automatic sequencer and a kit (BigDye Terminator Cycle Sequencing kit, Perkin-Elmer Applied Biosystems). 16S rDNA of the selected strains were analyzed for homology using the gene bank blast program (Fig. 1). As a result, the selected strain exhibited 99% homology with Streptomyces argentherus NRRL B-3230, which was finally confirmed to be a Streptomyces argentherus strain. Therefore, on July 6, 2009, it was deposited as a patent strain to the Korea Biotechnology Research Institute Gene Bank and was assigned accession number KCTC 11524BP.

Example  2: for various plant pathogens Antagonism  black

Phytopathogens include a variety of pathogens that cause significant damage to plants, Streptomyces scabies and Pythium. utimum KACC 40705 (root rot), Colletotrichum acutatum KACC 40042 (anthrax), Botrytis cinerea kacc 40573 (grey mold), cylindrocarpon destructans KACC 41077 (root rot), Sclerotinia sclerotiorum KACC 41065 (bacterial disease), Phytophthora Eight test specimens of cactorum KACC 40166 (pepper blight) and Rhizoctonia solani AG-2-2 (IV) KACC 40123 (root rot) (Mozalococcal disease) were obtained from the Korea Agricultural Microbiological Resource Center. Antagonistic assay was carried out to investigate the antimicrobial range.

After sterilizing potato agar medium (PDA, Difco), plate and mix 200 μl of the culture medium containing mycelia and spores of each pathogen in a medium at 50 ° C. (8 mm in diameter) and placed on a petri dish mixed with pathogens and incubated at 28 ° C. for 3-4 days to grow various pathogens. The effect of antagonistic microorganisms on various pathogens was investigated with a low support diameter, which is shown in Table 2 below.

As can be seen in Table 2, as a result of the antagonistic effect of the antagonistic microorganism Streptomyces Argenteorus strain of the present invention and the phytopathogenic bacteria, the strain of the present invention is the phytophthora pactrum, which is the causative bacterium It has excellent inhibitory effects against choletotricum akutat, the cause of anthrax, lysatonia solani, the cause of root rot, Pisium Ultimum, the cause of wear and mozzarella, and syllotini cilerothiorum, the cause of mycotic disease. In addition, it was confirmed that the inhibitory effect on Streptomyces scavig, the causative agent of potato beetle disease, and Botrytis cineraria, which causes ash fungus disease.

Streptomyces Argenteorus antimicrobial range against pathogenic fungi Test bacteria (fungus) Activity level Streptomyces scabies ) - Pythium ultimum ) +++++ Colletotrichum acutatum ) ++++ Botrytis Ceria cinerea ) + Cylindrocarpon destructans ) - Silerotinia Slerotinia sclerotiorum ) ++++ Phytophthora cactorum ) ++++ Rhizoctonia solani ) ++++ ++++: size of low support> 40 mm,
+++: 30-40 mm,
++: 20-30 mm,
+: 10-20 mm,
-: 10 mm or less

It showed excellent antagonism in most test strains. Especially, it has strong antimicrobial activity against Pisium Ultimum, Colletotricum Akutatum, Silerotinia Silerothiorum, Phytoprosola Cactrum, Raiztonia Solani, etc. These various antimicrobial activities suggest the possibility of application to various crops with one product. In addition, in order to re-verify the activity of Streptomyces argentherus, the antimicrobial activity of each test bacterium showing antimicrobial activity was confirmed. 3, 4, 5, 6, 7, 8, 9 and 10 as a result showing the antifungal activity of Streptomyces argentherus, respectively, 3, 4, 5, 6, 7, 8, 9 and As shown in 10, it was confirmed that the very excellent activity.

Example  3: Streptomyces Argenteorus  Mass culture

Antagonist microorganism Streptomyces argenteorus strain was cultured using a 2.5 L fermenter. The production medium used was 5 g of starch, 20 g of glucose, 25 g of soy flour, 1 g of juice extract, 4 g of yeast extract, 2 sodium chloride. g, potassium phosphate 0.25 g, and calcium carbonate 2 g were dissolved in 1 L of distilled water, and then a conventional liquid medium having a pH of 7.2 was prepared and used. Species culture was incubated in a 500 ml Erlenmeyer flask using a 100 ml liquid medium containing 5 g / L yeast extract, 5 g / L juicy extract and 10 g / L glucose. The culture conditions were shaken for 120 hours at 28 ℃ and 150 rpm, inoculated in a 2.5 L fermentation medium containing 1.5 L production medium 5% (v / v) of the culture medium containing Streptomyces argentherus and 28 ℃ Incubated for 120 hours at 150 rpm, 1 vvm condition (Fig. 9).

Example  4: Streptomyces Argenteorus  Confirm antimicrobial activity

Streptomyces argeneorus cultured in the same manner as in Example 3 was mixed with vermiculite so that the number of 10 ⁸ cells / 10 ha and sprayed on the pepper planting was investigated for the occurrence of pepper anthrax and late blight. As for the spraying method, foliar spraying was carried out at the first stage (before seedling) and at the second stage (before the rainy season), and none was treated as a control. The growth of pepper was analyzed by measuring the number of peppers per kilogram of the final pepper produced. As shown in Table 3, the microbial treatments were 53 peppers per kg, showing much better growth than the 64 control.

Pepper count / kg of control and treatment division
Control Treatment Count 64 53

1 is a 16S rDNA nucleotide sequence of Streptomyces argentherus of the present invention 446bp.

2 is an electron micrograph taken at 10,000 times magnification of Streptomyces argentherus of the present invention.

Figure 3 shows the antimicrobial activity of Streptomyces argenteorus of the present invention against Pythium ultimum .

Figure 4 shows the antimicrobial activity of Streptomyces argentherus of the present invention against Rhizoctonia solani .

Figure 5 shows the antimicrobial activity of Streptomyces argentherus of the present invention against Botritis cinerea .

Figure 6 shows the antimicrobial activity of Streptomyces argentherus of the present invention against Altenaria panax .

7 is Cylidrocarpon destructans destructans ) shows the antimicrobial activity of Streptomyces argentherus of the present invention.

Figure 8 shows the antimicrobial activity of Streptomyces argentherus of the present invention against Fusarium solani.

Figure 9 is a graph showing the growth curve of Streptomyces argentherus of the present invention over the incubation time.

Figure 10 is a photograph of the pepper laboratory to perform the present invention.

Figure 11 is a photograph of the pepper produced by treating the Streptomyces Argenteorus of the present invention.

Claims (10)

Strain of Streptomyces argenteolus subsp.toyonakensis KCTC 11524BP. According to claim 1, wherein said strain is characterized in that it has an antimicrobial activity against plant pathogens. delete 3. The plant pathogen of claim 2, wherein the plant pathogen is a causative agent of at least one plant disease selected from the group consisting of pepper blight, anthrax, root rot, ash fungus, erosion, mozzarella, spots, mycobacterium, and potato beetle. Strain characterized in that. 3. The plant pathogen of claim 2, wherein the plant pathogens are Phytoprosora chitorum, Colletotricum akutatum, Raiztonia Solani, Pisium Ultimum, Botrytis Ceria, Altenaria Panax, Cylindrocapon Destract And at least one selected from the group consisting of Tans, Fusarium Solani, Silerotinia silerothiorum, and Streptomyces scavig. A pesticide preparation for plant disease control comprising the cells, spores, culture medium or mixtures of the Streptomyces argentheor toyonaksis subspecies strain according to claim 1 as an active ingredient. A method of controlling plant diseases by using the pesticide preparation for plant diseases control according to claim 6. 8. The method of claim 7, wherein the plant disease is at least one plant disease selected from the group consisting of red pepper disease, anthrax, root rot, ash fungus, wear disease, mozzarella disease, spots, fungal disease and potato beetle disease. A method for preparing a pesticide preparation for plant disease control, comprising the step of culturing the Streptomyces argentheor toyonaksis subspecies strain according to claim 1. 10. The method of claim 9, wherein the culture is 120 ~ 180rpm at 25 ~ 30 ℃ using a medium to which yeast extract 3 ~ 6 g / L, juicy extract 3 ~ 6 g / L, glucose 8 ~ 12 g / L is added Manufacturing method, characterized in that carried out under conditions 3 to 7 days.

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