CN114854631B - Sponge-derived biocontrol streptomyces ITBB-ZK-a5 and application thereof - Google Patents
Sponge-derived biocontrol streptomyces ITBB-ZK-a5 and application thereof Download PDFInfo
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Abstract
The invention discloses a sponge-source Streptomyces sp. Biocontrol ITBB-ZK-a5 and a biocontrol product comprising the Streptomyces sp. Biocontrol ITBB-ZK-a5 for preventing and controlling diseases of crops and aquaculture animals, and the sponge-source Streptomyces sp. Biocontrol ITBB-ZK-a5 and the biocontrol product have prevention and control effects on various pathogenic bacteria. Also disclosed is the use of the sponge-derived biocontrol Streptomyces sp ITBB-ZK-a5 and biocontrol products comprising the same in the preparation of medicaments having the effects of preventing and controlling pathogenic fungi and pathogenic bacteria.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a sponge-derived biocontrol streptomyces ITBB-ZK-a5 and application thereof.
Background
Actinomycetes are a type of important microbial resource, which can produce abundant and diverse metabolites, and have been widely used in the agricultural and health fields, so that people increasingly pay attention to mining novel actinomycete resources with application potential from different environmental conditions. The special high-salt and low-nutrition environment of the ocean ensures that the marine actinomycetes have physiological structures and functions different from terrestrial populations and form unique metabolic pathways, so that abundant marine microorganism resources are created, and a huge agricultural microorganism resource library and an active metabolite library are formed. Sponges (marine sponges) are a large class of lower multicellular marine animals, which are open to the animal kingdom, and which have many tiny ducts through which seawater flows through the body, and which use the microorganisms and other organic fragments in the seawater as a food source, and many microorganisms, particularly those which cannot be digested, remain in the sponge during the sponge filter feeding process, so that a large number of microorganisms are enriched in the sponge and in the body surface. The sponge is a very simple and original marine organism, but can generate more abundant bioactive substances than other marine organism species, and is mainly characterized by the activities of antibiosis, antivirus, anti-tumor, antimalarial, insect expelling, enzyme inhibitor and the like.
Diseases caused by fungi and bacteria are the most main diseases in agricultural production and aquaculture, chemical pesticides are the most commonly used control methods, but the wide use of chemical pesticides not only can enhance the drug resistance of bacteria, but also can cause pollution to ecological environment, and more importantly, pesticide residues have a certain influence on human health. Therefore, finding a more green, efficient, safe control method is a common goal worldwide. Actinomycetes are the earliest microorganisms with biological control effect, the symbiotic actinomycetes with biological control effect on fungi and bacterial diseases are excavated from the sponge, the concept of green environmental protection is met, and the actinomycetes play an increasingly important role in the development of green microbial pesticides for crops and green microecological preparation key technologies facing the aquaculture industry.
Disclosure of Invention
The invention aims to provide a sponge-derived Streptomyces sp (Streptomyces sp.) ITBB-ZK-a5 and a biocontrol product comprising the Streptomyces sp.) ITBB-ZK-a5 for controlling crop and/or aquaculture animal diseases, wherein the sponge-derived Streptomyces sp (Streptomyces sp.) ITBB-ZK-a5 and the biocontrol product thereof have control effects on various pathogenic bacteria.
The invention also aims to provide application of the sponge-derived biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 or a biocontrol product comprising the biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 in preparation of medicines with effects of preventing and treating pathogenic fungi and/or pathogenic bacteria.
The first object of the present invention can be achieved by the following technical means: a strain of sponge-derived Streptomyces sp. ITBB-ZK-a5 having accession No. GDMCC No:62383, the date of preservation is 2022, 04 and 14, the preservation unit is the microorganism strain preservation center of Guangdong province, and the preservation address is the building 5 of No. 59 of the national institute of microbiology, academy of sciences of Guangdong province, xiuzhou, first, china.
The inventor acquires sponge of the southwest sandy islands of China, separates and purifies the sponge to obtain an antagonistic strain capable of effectively inhibiting various pathogenic bacteria, and names the antagonistic strain as Streptomyces sp.
The invention also provides a biocontrol product for preventing and treating crop and/or aquaculture animal diseases, which comprises the fermentation extract of the sponge-derived Streptomyces sp (ITBB-ZK-a 5) biocontrol Streptomyces sp and/or the sponge-derived Streptomyces sp (ITBB-ZK-a 5).
The second object of the present invention can be achieved by the following technical means: the application of the sponge-derived biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 or the biocontrol product in the preparation of medicaments for preventing and treating pathogenic fungi and/or pathogenic bacteria.
Preferably, the pathogenic fungi comprise one or a combination of a plurality of banana fusarium wilt, banana fusarium wilt No. 4 physiological race, banana anthracnose, papaya brown stalk rot, papaya canker, mango anthracnose, pepper bar spot, cowpea gray spot, cowpea stalk rot, date phomopsis, coconut fruit rot, rice blast, wheat scab, cotton verticillium, rubber anthracnose, cucumber root rot, dragon fruit rot, lychee cream phytophthora and dragon flower anthracnose.
Preferably, the pathogenic bacteria include one or a combination of several of bacterial leaf blight of rice, bacterial wilt of eggplant, vibrio harveyi, streptococcus iniae and streptococcus agalactiae.
Compared with the prior art, the invention has the following advantages:
(1) The Streptomyces sp ITBB-ZK-a5 has the characteristics of good antibacterial effect, wide antibacterial spectrum and stable control effect, and the antibacterial active component in the fermentation extract can be further used as a biological pesticide to be developed and applied to biological control of various agricultural biological diseases, and the bacteria and the biological control products such as the fermentation extract have important potential and good development prospect in the key technical development of green microbial pesticides for controlling the agricultural biological diseases;
(2) Compared with chemical pesticide ratios of biocontrol streptomyces ITBB-ZK-a5 and biocontrol products thereof, such as fermentation extracts and the like, the biocontrol streptomyces ITBB-ZK-a5 has the advantages of no residue, low toxicity, environmental friendliness and the like, and accords with the current environment-friendly concept.
Drawings
FIG. 1 is a colony morphology of actinomycetes ITBB-ZK-a5 obtained by screening in example 1;
FIG. 2 is a phylogenetic tree of the strain ITBB-ZK-a5 and related strains of example 2;
FIG. 3 shows the inhibitory effect of the strain ITBB-ZK-a5 of example 3 on 4 pathogenic fungi by streaking;
FIG. 4 shows the thermal stability of the inhibiting effect of the fermented extract of strain ITBB-ZK-a5 of example 6 on banana anthracnose at 40℃and 50℃in a 60℃water bath for 1 h;
FIG. 5 shows the photostability of the inhibiting effect of the fermented extract of strain ITBB-ZK-a5 of example 7 on banana anthracnose in UV light irradiation for 1h, 2h, 4h, 6h, 8h and 10h, respectively.
Detailed Description
EXAMPLE 1 isolation of strains
The sponge sample collected from the southwest island of China sea is separated by adopting a Gaoshi synthetic first culture medium as a separation plate and adopting a dilution coating method, and after culturing for 7 days at a constant temperature of 28 ℃, actinomycete strains ITBB-ZK-a5 are observed and selected to be cultured and purified on a Streptomyces culture medium No. 2 (ISP 2) and stored at a temperature of minus 20 ℃.
The culture medium for Gaoshi synthesis No. 1 is prepared from the following components in mass volume ratio: soluble starch 20.0g/L, sodium chloride 6.5g/L, ferrous sulfate 0.01g/L, potassium sulfate 1.0g/L, dipotassium hydrogen sulfate 0.5g/L, magnesium sulfate 0.5g/L, sea salt 17.5g/L, potassium dichromate 50 mu g/mL, pH 7.3+ -0.2, agar 20.0g/L (based on 1L water, the rest of culture medium is the same) was added when preparing the solid plate.
The ISP2 culture medium is prepared from the following components in mass volume ratio: yeast extract 4.0g/L, malt extract 10.0g/L, glucose 4.0g/L, sea salt 17.5g/L, pH 7.2.2+ -0.2, and agar 20.0g/L when preparing solid plate.
Actinomycete strain ITBB-ZK-a5 grows well on ISP2 solid medium, spores are generated after 2-4 d of culture at 28 ℃, the color of spore pile is nearly white after 5d of culture, and the colony morphology chart (7 d of culture) is shown in figure 1.
Example 2 molecular characterization of Strain ITBB-ZK-a5
Actinomycetes ITBB-ZK-a5 obtained in example 1 was inoculated into ISP2 solid medium, cultured at 28℃for 5d, single colony was picked up on fresh ISP2 medium plate for secondary purification culture for 5d, and the bacteria-carrying plate was directly submitted to Kunming division of Beijing qing department Biotechnology Co., ltd for 16S rDNA sequence determination.
The 16S rDNA gene sequence (shown as SEQ ID NO: 1) of the strain ITBB-ZK-a5 is as follows:
aaaggaggtgatccagccgcaccttccggtacggctaccttgttacgacttcgtcccaatcgccagtcccaccttcgacggctccctccacaagggttgggccaccggcttcgggtgttaccgactttcgtgacgtgacgggcggtgtgtacaaggcccgggaacgtattcaccgcagcaatgctgatctgcgattactagcgactccgacttcatggggtcgagttgcagaccccaatccgaactgagaccggctttttgagattcgctccacctcacggcttcgcagctcattgtaccggccattgtagcacgtgtgcagcccaagacataaggggcatgatgacttgacgtcgtccccaccttcctccgagttgaccccggcagtctcctgtgagtccccggcataacccgctggcaacacaggacaagggttgcgctcgttgcgggacttaacccaacatctcacgacacgagctgacgacagccatgcaccacctgtacaccgaccacaagggggcacccatctctggatgtttccggtgtatgtcaagccttggtaaggttcttcgcgttgcgtcgaattaagccacatgctccgccgcttgtgcgggcccccgtcaattcctttgagttttagccttgcggccgtactccccaggcggggaacttaatgcgttagctgcggcacggacaacgtggaatgtcgcccacacctagttcccaacgtttacggcgtggactaccagggtatctaatcctgttcgctccccacgctttcgctcctcagcgtcagtatcggcccagagatccgccttcgccaccggtgttcctcctgatatctgcgcatttcaccgctacaccaggaattccgatctcccctaccgaactctagcctgcccgtatcgaatgcagacccggagttaagccccgggctttcacatccgacgcgacaagccgcctacgagctctttacgcccaataattccggacaacgcttgcgccctacgtattaccgcggctgctggcacgtagttagccggcgcttcttctgcaggtaccgtcactcacgcttcttccctgctgaaagaggtttacaacccgaaggccgtcatccctcacgcggcgtcgctgcatcaggcttgcgcccattgtgcaatattccccactgctgcctcccgtaggagtctgggccgtgtctcagtcccagtgtggccggtcgccctctcaggccggctacccgtcgtcgccttggtaggccatcaccccaccaacaagctgataggccgcgggctcatcctgcaccgccggagctttccacacgtatcccatgcgggaacgcgtcatatccggtattagaccccgtttccagggcttgtcccagagtgcagggcagattgcccacgtgttactcacccgttcgccactaatccccggccgaaaccggttcatcgttcgacttgcatgtgttaagcacgccgccagcgttcgtcctgagccaggatcaaactctccgt1517。
the obtained sequences were subjected to BLAST alignment at the NCBI website, the 16S rDNA gene sequences of strains with similarity higher than 98% were selected as reference objects, multiple sequence alignment was performed in MEGA software, and the strains ITBB-ZK-a5 and Streptomyces malaysiens AB249918.1 were seen to be on the same clade through the phylogenetic tree constructed (as shown in fig. 2), and identified as Streptomyces sp., deposit No.: GDMCC No:62383, date of preservation: 2022, 04, 14, deposit unit: the collection address of the microorganism strain collection in Guangdong province: building 5 of No. 59 of 100 university of Mitsui in the View area of Guangzhou City of Guangdong province, academy of sciences of Guangdong province.
Example 3 test of antibacterial Effect of Strain ITBB-ZK-a5 on pathogenic fungi
The pathogenic fungi tested included banana Fusarium wilt (Fusarium oxysporum f.sp.cube, foc), banana Fusarium wilt No. 4 physiological race (Fusarium oxysporum f.sp.cube surgical race 4, foc 4), banana anthracnose (Colletotrichum musae), papaya anthracnose (Colletotrichum gloosporides penz.), papaya brown root rot (Phomopsis caricae-papaya efrak & cif.), papaya cocoa Fusarium dichotomum (Lasiodiplodia theobromae) (the primary pathogen causing papaya canker), mango anthracnose (Colletotrichum gloeosporioides), pepper anthracnose (Colletotrichum canci), pepper bar rot leaf spot (Corynespora cassiicola), cowpea gray stem (Corynespora cassiicola), cowpea anthracnose (Pythium aphanidermatum (ds.) Fitzp.), jujube dish-shaped trichoderma (Pestalotiopsis microspora), coconut root rot (4) (the primary pathogen causing papaya eflower & cif), papaya zebra (39384), corn (light yellow mould (4635), brown rot (463), brown rot (4639, and brown rot (463), and brown rot (5995.
The bacterial inhibition persistence of the strain ITBB-ZK-a5 obtained in example 1 was tested by streaking, and actinomycetes-fungi co-cultivation was selected: inoculating actinomycetes into a PDA solid culture dish by a streaking method, standing and culturing for 4d at the constant temperature of 28 ℃, respectively inoculating 15 pathogenic fungi blocks with the diameter of 7mm to the center of a PDA flat plate after the actinomycetes grow well in the culture dish, culturing for 4d at the position which is 2cm above and below the blocks, taking a flat plate without actinomycetes as a control group, standing and culturing for 7d, 14d and 30d at the constant temperature of 28 ℃, repeating each treatment group for 3 times, and recording the bacteriostasis rate of each period. The inhibition ratios are shown in Table 1 below, and the inhibition effect of some strains is shown in FIG. 3.
Antibacterial ratio (%) = [ (control group pathogen growth diameter-pathogen mass diameter) - (treatment group pathogen growth diameter-pathogen mass diameter) ]/(control group pathogen growth diameter-pathogen mass diameter) ×100%.
TABLE 1 results of antibacterial persistence of the strain ITBB-ZK-a5 (streaking method)
From the data in Table 1, the strain ITBB-ZK-a5 has strong inhibition effect on 15 plant pathogenic fungi in a counter culture period of 30 days, wherein the inhibition effect on important tropical economic crop pathogenic fungi banana anthracnose bacteria and papaya anthracnose bacteria is the best, the inhibition effect in 30 days reaches 98.71+/-1.28% and 98.07+/-1.92%, the inhibition effect is good, the inhibition effect of the dragon fruit rot bacteria is relatively low, and the inhibition rate is 72.43 +/-0.64%; in the whole, the strain ITBB-ZK-a5 has good antibacterial effect on 15 pathogenic fungi. Therefore, the strain has important potential and development prospect in the research and development of the key technology for preparing the green microbial pesticide for preventing and treating crop diseases.
The bacteria block counter method is adopted to perform bacteriostasis and persistence test on 6 pathogenic bacteria, 7mm actinomycete blocks are inoculated to two ends of a flat plate, meanwhile, 6 pathogenic fungi blocks with the diameter of 7mm are respectively inoculated to the center of a PDA flat plate, the flat plate without actinomycete is taken as a control group, standing culture is performed for 7d, 14d and 30d at the constant temperature of 28 ℃, each treatment group is repeated for 3 times, and the bacteriostasis rate of each period is recorded as shown in the following table 2.
TABLE 2 results of antibacterial persistence of the strain ITBB-ZK-a5 (method of block confrontation)
From the data in Table 2, it can be seen that the strain ITBB-ZK-a5 also has a better inhibition effect on 6 plant pathogenic fungi in a counter culture period of 30 days, wherein the inhibition effect on important economic crop pathogenic fungi cotton verticillium wilt is best, the inhibition rate in 30 days reaches 85.47+/-1.59%, the inhibition rate on main grain crop pathogenic fungi rice blast germ also reaches 79.48 +/-2.76%, and the inhibition rate is lower than that of wheat gibberella zeae, and the inhibition rate is 29.91+/-3.36%. Therefore, the strain has important potential and development prospect in the aspect of preparing green and environment-friendly microbial pesticide preparations for corresponding bacterial diseases.
Example 4 determination of the bacteriostasis Spectrum of the Strain ITBB-ZK-a5 on pathogenic bacteria
The test pathogenic bacteria include: bacterial blight of rice (Xanthomonas oryzae, XOO), bacterial wilt of eggplant (Ralstonia solanacearum), vibrio harveyi, streptococcus ragmitis (Streptococcus iniae), streptococcus agalactiae (Streptococcus agalactiae).
The method comprises the following steps: bacterial inhibition spectra of the pathogenic bacteria were studied with the strain ITBB-ZK-a5 obtained in example 1, and a plate-counter method was selected. Diluting the pathogenic bacteria liquid to light absorption value OD 600 The method comprises the steps of (1) absorbing 100 mu L of diluted pathogenic bacteria liquid, adding the diluted pathogenic bacteria liquid into a sterile culture dish, pouring about 15mL of agar culture medium, shaking uniformly, punching holes at the position, which is 10mm away from the edge, on the left side of the culture medium, adding 20 mu L of 20mg/mL kanamycin solution as a control, inverting an actinomycete block with the diameter of 7mm at the position, which is 10mm away from the edge on the right side, culturing at the constant temperature of 28 ℃ for 24-48 hours, observing whether a transparent bacteriostasis ring exists or not, and measuring the bacteriostasis diameter. The antibacterial results are shown in table 3 below.
TABLE 3 antibacterial diameter of the strain ITBB-ZK-a5 against pathogenic bacteria
From the data in Table 3, it can be seen that the bacterial strain ITBB-ZK-a5 has the most obvious inhibition effect on bacterial disease pathogenic bacteria of main grain crops, namely rice bacterial blight, and the antibacterial diameter is 36mm, and is equivalent to that of positive medicines; the inhibition effect on pathogenic bacteria streptococcus agalactiae and streptococcus iniae of tilapia of important aquaculture animals is equivalent to that of positive medicines, and the antibacterial effect on pathogenic bacteria vibrio harveyi of the important marine aquaculture animals is also achieved. Therefore, the strain has important application potential and development prospect in the development of the key technology of green microecological preparations in the aquaculture industry.
Example 5 fermentation of Strain ITBB-ZK-a5 and acquisition of fermented extract thereof
Activating the strain ITBB-ZK-a5 obtained in example 1 by ISP2 plates, inoculating in TSB liquid medium, and shaking culturing at 28 ℃ and 180rpm for 2d; inoculating into 3L liquid fermentation medium according to 10% (volume percentage) inoculum size, and shake culturing at 28deg.C and 180rpm for 13d to obtain fermentation broth; extracting the fermentation broth with equal volume of ethyl acetate (3L), and concentrating under reduced pressure at 45deg.C to obtain extract (5 g) which is strain ITBB-ZK-a5 fermentation extract.
The TSB liquid culture medium is prepared from the following components in weight-volume ratio: 17g/L of tryptone, 3g/L of soybean papain hydrolysate, 5g/L of sodium chloride, 2.5g/L of dipotassium hydrogen phosphate, 2.5g/L of glucose and pH 7.3+/-0.2.
The liquid fermentation medium is prepared from the following components in percentage by weight and volume: 20g/L of soluble starch, 5g/L of peptone, 3g/L of malt extract, 3g/L of yeast extract, 10g/L of glucose and CaCO 3 1 g/L，pH7.0。
Example 6 thermal stability test of fermented extracts of Strain ITBB-ZK-a5 against plant pathogenic bacteria
The fermented extract prepared in example 5 was dissolved in dimethyl sulfoxide (DMSO) to give 10mg/mL of a sample solution, and the solution was placed in water baths at 40℃and 50℃and 60℃for 1 hour, respectively. The bacterial inhibition activity of actinomycetes is measured by a flat filter paper sheet method by taking banana anthracnose as an indicator bacterium: inoculating 7mm diameter banana anthracnose bacteria blocks to the center of a PDA flat plate, placing a filter paper sheet with the diameter of 7mm at the position 2cm above and below the bacteria blocks, taking carbendazim (effective content 98%), chlorothalonil (effective content 75%) and myclobutanil (effective content 40%) as positive control, taking a banana anthracnose bacteria flat plate without actinomycetes as a control group, repeating each treatment for 3 times, culturing for 10 days at the constant temperature of 28 ℃, observing and recording the bacteriostasis rate; the bacteriostasis rate is shown in the following table 4, and the bacteriostasis effect is shown in fig. 4.
TABLE 4 thermal stability of fermented extracts of the strain ITBB-ZK-a5 against plant pathogens
As can be seen from the data in Table 4, the improvement of the temperature has slight influence on the antibacterial effect of 3 positive drugs and the bacterial strain ITBB-ZK-a5, the antibacterial effect of the bacterial strain ITBB-ZK-a5 is equivalent to that of myclobutanil, the inhibition effect of the bacterial strain ITBB-ZK-a5 is stronger than that of chlorothalonil, and the bacterial strain ITBB-ZK-a5 has better stability to temperature change.
EXAMPLE 7 photostability test of fermented extract of Strain ITBB-ZK-a5 against plant pathogenic bacteria
The fermented extract prepared in example 5 was dissolved with DMSO to give a 10mg/mL sample solution, and the solution was irradiated under an ultraviolet lamp for 1h, 2h, 4h, 6h, 8h, and 10h, respectively. The inhibiting activity of actinomycetes is measured by a flat filter paper sheet method by taking banana anthracnose as an indicator bacterium: inoculating 7mm diameter banana anthracnose bacteria block to the center of a PDA flat plate, placing a 7mm diameter filter paper sheet at a position 2cm above and below the bacteria block, taking carbendazim, chlorothalonil and myclobutanil as positive control, taking a banana anthracnose bacteria flat plate without actinomycetes as a control group, repeating each treatment for 3 times, and culturing for 10 days at a constant temperature of 28 ℃; the bacteriostasis rate is shown in the following table 5, and the bacteriostasis effect is shown in fig. 5.
TABLE 5 photostability of fermented extracts of the strain ITBB-ZK-a5 against plant pathogens
As can be seen from the data in Table 5, along with the extension of the ultraviolet irradiation time, the antibacterial rate of the fermented extract of the strain ITBB-ZK-a5 has a reduced trend, but still has a better antibacterial effect, the antibacterial rate is about 58 to 70 percent, and the antibacterial activity of the antibacterial extract is equivalent to that of myclobutanil and is stronger than that of chlorothalonil. Thus, the strain ITBB-ZK-a5 can exert a stabilizing effect in controlling diseases caused by such above fungi and bacteria.
It should be noted that the above-mentioned embodiments are merely for further explanation of the present invention and are not intended to limit the present invention, and any modifications or variations within the meaning and scope of the technical solutions of the present invention, which are considered to be included in the scope of the present invention by those skilled in the art.
Sequence listing
<110> Tropical biotechnology institute of Tropical agricultural academy of sciences in China
<120> Streptomyces biocontrol ITBB-ZK-a5 from sponge and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1517
<212> DNA
<213> Streptomyces biocontrol (Streptomyces sp)
<400> 1
aaaggaggtg atccagccgc accttccggt acggctacct tgttacgact tcgtcccaat 60
cgccagtccc accttcgacg gctccctcca caagggttgg gccaccggct tcgggtgtta 120
ccgactttcg tgacgtgacg ggcggtgtgt acaaggcccg ggaacgtatt caccgcagca 180
atgctgatct gcgattacta gcgactccga cttcatgggg tcgagttgca gaccccaatc 240
cgaactgaga ccggcttttt gagattcgct ccacctcacg gcttcgcagc tcattgtacc 300
ggccattgta gcacgtgtgc agcccaagac ataaggggca tgatgacttg acgtcgtccc 360
caccttcctc cgagttgacc ccggcagtct cctgtgagtc cccggcataa cccgctggca 420
acacaggaca agggttgcgc tcgttgcggg acttaaccca acatctcacg acacgagctg 480
acgacagcca tgcaccacct gtacaccgac cacaaggggg cacccatctc tggatgtttc 540
cggtgtatgt caagccttgg taaggttctt cgcgttgcgt cgaattaagc cacatgctcc 600
gccgcttgtg cgggcccccg tcaattcctt tgagttttag ccttgcggcc gtactcccca 660
ggcggggaac ttaatgcgtt agctgcggca cggacaacgt ggaatgtcgc ccacacctag 720
ttcccaacgt ttacggcgtg gactaccagg gtatctaatc ctgttcgctc cccacgcttt 780
cgctcctcag cgtcagtatc ggcccagaga tccgccttcg ccaccggtgt tcctcctgat 840
atctgcgcat ttcaccgcta caccaggaat tccgatctcc cctaccgaac tctagcctgc 900
ccgtatcgaa tgcagacccg gagttaagcc ccgggctttc acatccgacg cgacaagccg 960
cctacgagct ctttacgccc aataattccg gacaacgctt gcgccctacg tattaccgcg 1020
gctgctggca cgtagttagc cggcgcttct tctgcaggta ccgtcactca cgcttcttcc 1080
ctgctgaaag aggtttacaa cccgaaggcc gtcatccctc acgcggcgtc gctgcatcag 1140
gcttgcgccc attgtgcaat attccccact gctgcctccc gtaggagtct gggccgtgtc 1200
tcagtcccag tgtggccggt cgccctctca ggccggctac ccgtcgtcgc cttggtaggc 1260
catcacccca ccaacaagct gataggccgc gggctcatcc tgcaccgccg gagctttcca 1320
cacgtatccc atgcgggaac gcgtcatatc cggtattaga ccccgtttcc agggcttgtc 1380
ccagagtgca gggcagattg cccacgtgtt actcacccgt tcgccactaa tccccggccg 1440
aaaccggttc atcgttcgac ttgcatgtgt taagcacgcc gccagcgttc gtcctgagcc 1500
aggatcaaac tctccgt 1517
Claims (5)
1. Streptomyces biocontrol strain from spongeStreptomycessp.) ITBB-ZK-a5, characterized by: the deposit number is GDMCC No:62383, a date of deposit of 2022, 04, 14, a deposit unit of GuangdongThe collection address of the microorganism strain collection center is the building 5 of No. 59 of No. 100 of Mitsui No. 100 of Xiuzhou district of Guangzhou city of Guangdong province, and the institute of microorganisms of the academy of sciences of Guangdong province.
2. A biocontrol product for preventing and treating diseases of crops and/or aquaculture animals, which is characterized by comprising the sponge-source biocontrol streptomyces as described in claim 1Streptomyces sp.) ITBB-ZK-a5 and/or Streptomyces biocontrol of sponge origin as defined in claim 1Streptomycessp.) fermented extracts of ITBB-ZK-a 5; the fermentation extract is prepared from sponge-derived streptomyces biocontrol strainStreptomycessp.) the fermentation broth of ITBB-ZK-a5 was extracted with an equal volume of ethyl acetate and concentrated to dryness under reduced pressure at 45 ℃.
3. The sponge-derived Streptomyces biocontrol strain of claim 1Streptomycessp.) ITBB-ZK-a5 or the biocontrol product of claim 2 for use in the manufacture of a medicament having an effect of controlling pathogenic fungi and/or pathogenic bacteria.
4. A use according to claim 3, characterized in that: the pathogenic fungi comprise banana fusarium wiltFusarium oxysporum f.sp.cubenseFoc physiological race No. 4 of banana fusarium wiltFusarium oxysporumf.sp.cubense tropical race4, foc4, banana anthracnoseColletotrichum musaeBacillus anthracisColletotrichum gloeosporioidesPenz, papaya brown pedicle rot germPhomopsis caricae- papayae Fetrak&Cif, chaenomeles sinensis, conidiomyces lanuginosusLasiodiplodia theobromaeMango anthracnoseColletotrichum gloeosporioidesColletotrichum glomeratum of capsicumColletotrichum capsiciLeuconostoc capsiciCorynespora cassiicolaGray spot germ of cowpeaCorynespora cassiicola(Berk. Et Curt.) Wei, cowpea stem rot pathogenPythium aphanidermatum(eds.) Fitzp., mucor pulmonale, choerospondiasPestalotiopsis microsporaCocois radiata mouldThielaviopsis paradoxaWater ofRice blast bacteriaPyricularia oryzaeWheat scab fungusFusarium graminearumschw, cotton verticilliumVerticillium dahliaeColletotrichum gloeosporioides (L.) persColletotrichum acutatumMelon root rot disease bacteriaFusarium solaniFruit rot of dragon fruitGilbertella persicariaPhytophthora litchiiPeronophythora litchiiAnd anthracnose pathogen of LonghuaColletotrichum australianumOne or a combination of several of them.
5. A use according to claim 3, characterized in that: the pathogenic bacteria include bacterial leaf blight of riceXanthomonas oryzaepv.oryzaeLaurella solanacearumRalstonia solanacearumVibrio harveyi (Vibrio harveyi)Vibrio harveyi) Streptococcus iniae (Streptococcus iniae)Streptococcus iniae) And Streptococcus agalactiaeStreptococcus agalactiae) One or a combination of several of them.
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| CN114933997B (en) * | 2022-06-13 | 2023-06-23 | 中国热带农业科学院热带生物技术研究所 | Coral-derived symbiotic streptomyces SH001 and application thereof |
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