CN114854631A - Sponge-derived biocontrol streptomyces ITBB-ZK-a5 and application thereof - Google Patents
Sponge-derived biocontrol streptomyces ITBB-ZK-a5 and application thereof Download PDFInfo
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Abstract
The invention discloses a sponge-derived biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 and a biocontrol product for preventing and treating crop and aquaculture animal diseases, which comprises the biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5, wherein the sponge-derived biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 and the biocontrol product have control effects on various pathogenic bacteria. Also discloses application of the sponge-derived biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 and a biocontrol product comprising the biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 in preparation of a medicament with pathogenic fungus and pathogenic bacteria preventing and treating effects.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a sponge-derived biocontrol streptomyces ITBB-ZK-a5 and application thereof.
Background
Actinomycetes are a class of microbial resources with important values, can produce abundant and various metabolites, and have been widely used in the fields of agriculture and health, so people increasingly attach importance to the discovery of novel actinomycetes resources with application potential from different environmental conditions. The special environments of high salt, low nutrition and the like of the ocean lead the ocean actinomycetes to have physiological structures and functions different from terrestrial populations and form unique metabolic pathways, so that abundant ocean microorganism resources are created, and the ocean actinomycetes become a huge agricultural microorganism resource library and an active metabolite library. The sponge (marine sponge) belongs to the porous animal door (Porifera) of the animal kingdom, is a large class of low-grade multicellular marine animals, and the body surface of the sponge has a plurality of small pipelines, seawater flows through the body through the pipelines, microorganisms and other organic debris in the seawater are used as food sources, and in the sponge filter feeding process, a plurality of microorganisms, particularly microorganisms which can not be digested, are reserved in the sponge, so that a large number of microorganisms are enriched in the sponge and on the body surface. Although a very simple and primitive marine organism, sponges produce a greater abundance of biologically active substances than other marine organism species, primarily in terms of antibacterial, antiviral, antitumor, antimalarial, anthelmintic, and enzyme inhibitor activities.
Diseases caused by fungi and bacteria are the most main diseases in agricultural production and aquaculture, and chemical pesticides are the most commonly used prevention and treatment methods, but the wide use of chemical pesticides can not only enhance the drug resistance of germs, but also pollute the ecological environment, and more importantly, pesticide residues also have certain influence on the health of human beings. Therefore, it is a common goal all over the world to find a more green, efficient, and safe control method. The actinomycetes are the microorganisms with biological control function discovered at the earliest, and the symbiotic actinomycetes with biological control effect on fungi and bacterial diseases are excavated from the sponge, so that the actinomycetes accord with the concept of green environmental protection, and play an increasingly important role in the research and development of crop green microbial pesticides and key technologies of green microbial preparations facing the aquaculture industry.
Disclosure of Invention
The invention aims to provide a sponge-derived biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 and a biocontrol product for preventing and treating diseases of crops and/or aquaculture animals, wherein the biocontrol product comprises the biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5, and the sponge-derived biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 and the biocontrol product thereof have control effects on various pathogenic bacteria.
The invention also aims to provide application of the sponge-derived biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 or a biocontrol product comprising the biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 in preparation of a medicament with an effect of preventing and treating pathogenic fungi and/or pathogenic bacteria.
The first object of the present invention can be achieved by the following technical solutions: a sponge-derived biocontrol Streptomyces sp ITBB-ZK-a5 strain with the deposit number of GDMCC No: 62383 it has a preservation date of 2022, 14/04, and the preservation unit is Guangdong province microorganism strain preservation center, and the preservation address is Guangdong province, Guangzhou city, Vibrio junior 100 college No. 59 building No. 5 building, Guangdong province academy of sciences microbiology.
The inventor obtains an antagonistic strain capable of effectively inhibiting various pathogenic bacteria by collecting sponges of the Hsisha Islands in south China sea, separating and purifying, and in the embodiment of the invention, the antagonistic strain is named as Streptomyces bio-control (ITBB) -ZK-a5, which is abbreviated as ITBB-ZK-a 5.
The invention also provides a biocontrol product for controlling crop and/or aquaculture animal diseases, which comprises the sponge-derived Streptomyces biocontrol (Streptomyces sp.) ITBB-ZK-a5 and/or the sponge-derived Streptomyces biocontrol (Streptomyces sp.) ITBB-ZK-a5 fermentation extract.
The second object of the present invention can be achieved by the following technical solutions: the application of the sponge-derived biocontrol Streptomyces sp ITBB-ZK-a5 or the biocontrol product in preparing medicines with the effect of preventing and treating pathogenic fungi and/or pathogenic bacteria.
Preferably, the pathogenic fungi comprise one or a combination of more of banana fusarium oxysporum, No. 4 physiological race of banana fusarium oxysporum, banana colletotrichum, papaya brown stalk rot, papaya canker, mango colletotrichum, capsicum clavatum, cowpea gray spot, cowpea stem rot, date palm pestalotiopsis, coconut fruit rot, rice blast, wheat gibberellic disease, cotton verticillium wilt, rubber colletotrichum, cucumber root rot, pitaya rot, peronospora littoralis and dragon boat flower colletotrichum.
Preferably, the pathogenic bacteria comprise one or more of rice bacterial blight, bacterial wilt eggplant, vibrio harveyi, streptococcus iniae and streptococcus agalactiae.
Compared with the prior art, the invention has the following advantages:
(1) the obtained Streptomyces biocontrol Streptomyces (Streptomyces sp.) ITBB-ZK-a5 has the characteristics of good antibacterial effect, wider antibacterial spectrum and stable control effect, and antibacterial active components in the fermentation extract of the Streptomyces biocontrol Streptomyces have the advantages that the bacteria can be further developed and applied to biological control of various agricultural biological diseases as biological pesticides, and the bacteria and the biocontrol products such as the fermentation extract of the bacteria have important potential and better development prospect in the research and development of key technologies for preparing green microbial pesticides for controlling agricultural biological diseases;
(2) compared with chemical pesticides, the biocontrol streptomyces strain ITBB-ZK-a5 and the biocontrol products thereof such as fermentation extracts have the advantages of no residue, low toxicity, environmental friendliness and the like, and better accord with the current green environmental protection concept.
Drawings
FIG. 1 is a colony morphology of actinomycetes ITBB-ZK-a5 obtained by screening in example 1;
FIG. 2 is a phylogenetic tree of the strain ITBB-ZK-a5 and related strains in example 2;
FIG. 3 shows the inhibitory effect of the strain ITBB-ZK-a5 on 4 pathogenic fungi in example 3 by streaking;
FIG. 4 is the thermostability of the inhibitory effect of the fermented extract of the strain ITBB-ZK-a5 in example 6 on banana colletotrichum at 40 ℃ and 50 ℃ in a water bath at 60 ℃ for 1 hour;
FIG. 5 shows the photostability of the inhibitory effect of the fermented extract of the strain ITBB-ZK-a5 in example 7 on banana anthracnose pathogen after being irradiated under an ultraviolet lamp for 1h, 2h, 4h, 6h, 8h and 10h, respectively.
Detailed Description
EXAMPLE 1 Strain isolation
A sponge sample collected from the Xisha Islands of the south China sea selects a Gao's synthetic culture medium I as a separation plate, is separated by a dilution coating method, is cultured at a constant temperature of 28 ℃ for 7 days, is observed and picked from actinomycete ITBB-ZK-a5 to a culture medium No. 2 (ISP2) of streptomycete, is cultured and purified, and is stored at a temperature of-20 ℃ in a glycerol tube.
The Gao's synthetic No. one culture medium is prepared from the following components in mass-volume ratio: 20.0g/L of soluble starch, 6.5g/L of sodium chloride, 0.01g/L of ferrous sulfate, 1.0g/L of potassium sulfate, 0.5g/L of dipotassium hydrogen sulfate, 0.5g/L of magnesium sulfate, 17.5g/L of sea salt, 50 mu g/mL of potassium dichromate and pH of 7.3 +/-0.2, and 20.0g/L of agar (1L of water is used as a reference, and the rest of the culture medium is the same) is added when preparing a solid plate.
The ISP2 culture medium is prepared from the following components in mass-volume ratio: yeast extract 4.0g/L, malt extract 10.0g/L, glucose 4.0g/L, sea salt 17.5g/L, pH 7.2.2 + -0.2 g/L, and agar 20.0g/L is added when preparing solid plate.
The actinomycete strain ITBB-ZK-a5 grows well on an ISP2 solid medium, spores begin to generate after 2-4 days of culture at 28 ℃, the color of a spore pile is close to white after 5 days of culture, and a colony morphology chart (culture 7d) is shown in FIG. 1.
Example 2 molecular characterization of Strain ITBB-ZK-a5
The actinomycetes ITBB-ZK-a5 obtained in example 1 was inoculated into an ISP2 solid medium, cultured at 28 ℃ for 5 days, a single colony was picked up and subjected to secondary purification culture on a fresh ISP2 medium plate for 5 days, and the plate with the actinomycetes was directly handed over to Kunming Branch of Biotech Limited, Beijing Oncology, for 16S rDNA sequencing.
The 16S rDNA gene sequence (shown as a sequence table SEQ ID NO: 1) of the strain ITBB-ZK-a5 is as follows:
aaaggaggtgatccagccgcaccttccggtacggctaccttgttacgacttcgtcccaatcgccagtcccaccttcgacggctccctccacaagggttgggccaccggcttcgggtgttaccgactttcgtgacgtgacgggcggtgtgtacaaggcccgggaacgtattcaccgcagcaatgctgatctgcgattactagcgactccgacttcatggggtcgagttgcagaccccaatccgaactgagaccggctttttgagattcgctccacctcacggcttcgcagctcattgtaccggccattgtagcacgtgtgcagcccaagacataaggggcatgatgacttgacgtcgtccccaccttcctccgagttgaccccggcagtctcctgtgagtccccggcataacccgctggcaacacaggacaagggttgcgctcgttgcgggacttaacccaacatctcacgacacgagctgacgacagccatgcaccacctgtacaccgaccacaagggggcacccatctctggatgtttccggtgtatgtcaagccttggtaaggttcttcgcgttgcgtcgaattaagccacatgctccgccgcttgtgcgggcccccgtcaattcctttgagttttagccttgcggccgtactccccaggcggggaacttaatgcgttagctgcggcacggacaacgtggaatgtcgcccacacctagttcccaacgtttacggcgtggactaccagggtatctaatcctgttcgctccccacgctttcgctcctcagcgtcagtatcggcccagagatccgccttcgccaccggtgttcctcctgatatctgcgcatttcaccgctacaccaggaattccgatctcccctaccgaactctagcctgcccgtatcgaatgcagacccggagttaagccccgggctttcacatccgacgcgacaagccgcctacgagctctttacgcccaataattccggacaacgcttgcgccctacgtattaccgcggctgctggcacgtagttagccggcgcttcttctgcaggtaccgtcactcacgcttcttccctgctgaaagaggtttacaacccgaaggccgtcatccctcacgcggcgtcgctgcatcaggcttgcgcccattgtgcaatattccccactgctgcctcccgtaggagtctgggccgtgtctcagtcccagtgtggccggtcgccctctcaggccggctacccgtcgtcgccttggtaggccatcaccccaccaacaagctgataggccgcgggctcatcctgcaccgccggagctttccacacgtatcccatgcgggaacgcgtcatatccggtattagaccccgtttccagggcttgtcccagagtgcagggcagattgcccacgtgttactcacccgttcgccactaatccccggccgaaaccggttcatcgttcgacttgcatgtgttaagcacgccgccagcgttcgtcctgagccaggatcaaactctccgt1517。
BLAST comparison is carried out on the obtained sequences at an NCBI website, a 16S rDNA gene sequence of a strain with the similarity of more than 98% is selected as a reference object, multi-sequence comparison is carried out in MEGA software, a constructed phylogenetic tree (shown in figure 2) can show that the strain ITBB-ZK-a5 and Streptomyces malaysiens AB249918.1 are on the same clade, and the strain is identified as Streptomyces (Streptomyces sp.), and the accession number is as follows: GDMCC No: 62383, date of deposit: 14/04/2022, depository: guangdong province microorganism strain preservation center, preservation address: the microbial research institute of the academy of sciences of Guangdong province, Guangzhou, Youzhou, Mr. No. 59 building 5 of the Zhou Dazhou school, Shih-100, of the Piezui district of Guangzhou.
Example 3 duration of bacteriostasis of the pathogenic fungus test by the Strain ITBB-ZK-a5
Pathogenic fungi for testing include banana wilt (Fusarium oxysporum f.sp.cubense, Foc), banana wilt 4 (Fusarium oxysporum f.sp.cubense tropical race 4, Foc4), banana anthracnose (Colletotrichum mosae), papaya anthracnose (Colletotrichum gloeosporioides sponz.), papaya brown rot (Phomopsis carota-payaeFetrak & Cif.), papaya trichum trichoderma (lasioderma theobromae) (main pathogen causing papaya canker), mango anthracnose (Fusarium gloeosporioides), pepper anthracnose (Colletotrichum trichothecoides), pepper rot (Fusarium oxysporum), pepper stem rot of common pea vine (Fusarium oxysporum), pepper rot of common pea vine (Fusarium oxysporum), common pea vine, common rot of common fungus Wheat scab (Fusarium graminearum schw.), cotton Verticillium wilt (Verticillium dahliae), Colletotrichum gloeosporioides (Colletotrichum aculatum), cucurbit root rot (Fusarium solani), dragon fruit rot (Gilbertella persica), peronospora litchii (Peronophythora litchii), and dragon boat flower anthracnose (Colletotrichum australium).
The bacterial strain ITBB-ZK-a5 obtained in example 1 was tested for its bacteriostatic duration by streaking, and actinomycetes-fungi coculture was selected: inoculating actinomycetes into a PDA (personal digital assistant) solid culture dish by a scribing method, carrying out constant-temperature static culture for 4d at 28 ℃, respectively inoculating 15 pathogenic fungus blocks with the diameter of 7mm in the center of a PDA flat plate after the growth of the actinomycetes in the culture dish is good, setting actinomycetes belts for culturing for 4d at positions 2cm above and below the fungus blocks, taking the flat plate without actinomycetes as a control group, carrying out constant-temperature static culture for 7d, 14d and 30d at 28 ℃, repeating the steps for 3 times in each treatment group, and recording the bacteriostasis rate in each period. The inhibition rates are shown in Table 1 below, and the inhibition effects of some strains are shown in FIG. 3.
The bacteriostatic rate (%) - (control pathogen growth diameter-pathogen block diameter) - (treatment pathogen growth diameter-pathogen block diameter) ]/(control pathogen growth diameter-pathogen block diameter) × 100%.
TABLE 1 results of bacteriostatic persistence of the strain ITBB-ZK-a5 (line marking)
The data in Table 1 show that the strain ITBB-ZK-a5 has strong inhibition effect on 15 plant pathogenic fungi in a 30-day opposite culture period, wherein the inhibition effect on pathogenic fungi banana anthracnose and papaya anthracnose of important tropical economic crops is the best, the inhibition effect within 30 days reaches 98.71 +/-1.28 percent and 98.07 +/-1.92 percent, the antibacterial duration is also good, the relative inhibition effect on the pitaya pythium is lower, and the inhibition rate is 72.43 +/-0.64 percent; in general, the strain ITBB-ZK-a5 has better bacteriostatic persistence on 15 pathogenic fungi. Therefore, the bacillus has important potential and development prospect in the key technology research and development of preparing green microbial pesticide for preventing and treating crop diseases.
A bacterium block confronting method is adopted to carry out bacteriostasis persistent effect test on 6 pathogenic bacteria, 7mm actinomycete blocks are inoculated to two ends of a flat plate, 6 pathogenic fungi blocks with the diameter of 7mm are respectively inoculated in the center of a PDA flat plate, the flat plate without actinomycete is taken as a control group, standing culture is carried out for 7d, 14d and 30d at the constant temperature of 28 ℃, each treatment group is repeated for 3 times, and the bacteriostasis rate of each period is recorded, and is shown in the following table 2.
TABLE 2 results of antibacterial persistence of the strain ITBB-ZK-a5 (Block confrontation method)
The data in Table 2 show that the strain ITBB-ZK-a5 has a good inhibition effect on 6 plant pathogenic fungi in a 30-day opposite culture period, wherein the inhibition effect on the important economic crop pathogenic fungi verticillium dahliae is the best, the inhibition rate within 30 days reaches 85.47 +/-1.59%, the inhibition rate on the main food crop pathogenic fungi rice blast is 79.48 +/-2.76%, the inhibition rate with lower inhibition rate is wheat scab, and the inhibition rate is 29.91 +/-3.36%. Therefore, the bacillus subtilis has important potential and development prospect in the aspect of preparing green and environment-friendly microbial pesticide medicaments for corresponding pathogenic bacteria and diseases.
EXAMPLE 4 determination of the bacteriostatic Profile of pathogenic bacteria by Strain ITBB-ZK-a5
The pathogenic bacteria tested included: rice bacterial blight (XOO), Ralstonia solanacearum (Ralstonia solanacearum), Vibrio harveyi (Vibrio harveyi), Streptococcus iniae (Streptococcus iniae), Streptococcus agalactiae (Streptococcus agalactiae).
The method comprises the following steps: the bacterial inhibition spectrum of the strain ITBB-ZK-a5 obtained in example 1 was investigated for pathogenic bacteria, and a plate counter method was selected. Diluting the pathogenic bacteria liquid to light absorption value OD 600 Sucking 100 mu L of diluted pathogenic bacteria liquid, adding the diluted pathogenic bacteria liquid into a sterile culture dish, pouring about 15mL of agar culture medium, shaking up, after the culture medium is solidified, punching a hole at the position, which is 10mm away from the edge, of the left side of the culture medium, adding 20 mu L of 20mg/mL kanamycin solution as a control, inverting an actinomycete block with the diameter of 7mm at the position, which is 10mm away from the edge, of the right side, culturing at the constant temperature of 28 ℃ for 24-48 h, observing whether a transparent bacteriostatic ring exists, and measuring the bacteriostatic diameter. The bacteriostatic results are shown in table 3 below.
TABLE 3 bacteriostatic diameter of the strain ITBB-ZK-a5 against pathogenic bacteria
The data in the table 3 show that the bacterial strain ITBB-ZK-a5 has the most obvious inhibition effect on the bacterial disease pathogenic bacteria rice bacterial blight germ of the main food crops, the inhibition diameter is 36mm, and the inhibition effect is equivalent to that of a positive medicament; the inhibition effect on tilapia pathogenic bacteria streptococcus agalactiae and streptococcus iniae of important aquaculture animals is equivalent to that of positive medicaments, and the inhibition effect on pathogenic bacteria vibrio harveyi of important seawater aquaculture animals is also equivalent to that of positive medicaments. Therefore, the bacillus has important application potential and development prospect in the key technology research and development of green microecological preparations in aquaculture industry.
Example 5 fermentation of the Strain ITBB-ZK-a5 and obtaining of its fermentation extract
The strain ITBB-ZK-a5 obtained in example 1 was activated by an ISP2 plate, inoculated into a TSB liquid medium, and subjected to shaking culture at 28 ℃ and 180rpm for 2 d; inoculating into 3L liquid fermentation culture medium according to 10% (volume percentage content), and performing shaking culture at 28 deg.C and 180rpm for 13d to obtain fermentation liquid; the fermentation broth was extracted with equal volume of ethyl acetate (3L) and concentrated to dryness at 45 deg.C under reduced pressure to give extract (5g), which was the fermented extract of strain ITBB-ZK-a 5.
The TSB liquid culture medium is prepared from the following components in percentage by weight and volume: 17g/L tryptone, 3g/L soybean papain hydrolysate, 5g/L sodium chloride, 2.5g/L dipotassium hydrogen phosphate and 2.5g/L glucose, and the pH value is 7.3 +/-0.2.
The liquid fermentation medium is prepared from the following components in percentage by weight and volume: 20g/L of soluble starch, 5g/L of peptone, 3g/L of malt extract, 3g/L of yeast extract, 10g/L of glucose and CaCO 3 1 g/L，pH7.0。
EXAMPLE 6 Strain ITBB-ZK-a5 fermentation extract thermostability test against phytopathogens
The fermentation extract prepared in example 5 was dissolved in dimethyl sulfoxide (DMSO) to obtain a sample solution with a concentration of 10mg/mL, and the solution was then subjected to water bath at 40 deg.C, 50 deg.C, and 60 deg.C for 1 h. The bacteriostatic activity of actinomycetes is determined by a flat filter paper sheet method by taking banana colletotrichum lagenarium as an indicator bacterium: inoculating a banana colletotrichum gloeosporioides bacterial block with the diameter of 7mm to the center of a PDA (personal digital assistant) plate, placing a filter paper sheet with the diameter of 7mm at a position 2cm away from the upper part and the lower part of the bacterial block, taking carbendazim (with the effective content of 98%), chlorothalonil (with the effective content of 75%) and myclobutanil (with the effective content of 40%) as positive control, taking a banana colletotrichum gloeosporioides plate without inoculating actinomycetes as a control group, repeating each treatment for 3 times, carrying out constant-temperature inverted culture at 28 ℃ for 10 days, and observing and recording the bacteriostasis rate; the bacteriostatic rate is shown in table 4 below, and the bacteriostatic effect is shown in fig. 4.
TABLE 4 thermostability of fermented extracts of strain ITBB-ZK-a5 against phytopathogens
As can be seen from the data in Table 4, the temperature increase has a slight influence on the bacteriostatic effects of the 3 positive drugs and the strain ITBB-ZK-a5, the bacteriostatic effect of the strain ITBB-ZK-a5 is equivalent to that of myclobutanil, and the bacteriostatic effect is stronger than that of chlorothalonil and has better stability to the temperature change.
EXAMPLE 7 photostability test of fermented extract of Strain ITBB-ZK-a5 against phytopathogens
The fermentation extract prepared in example 5 was dissolved in DMSO to obtain a sample solution with a concentration of 10mg/mL, and the sample solution was irradiated under an ultraviolet lamp for 1h, 2h, 4h, 6h, 8h, and 10h, respectively. The inhibition activity of actinomycetes is determined by a flat filter paper sheet method by taking banana colletotrichum lagenarium as an indicator bacterium: preparing a banana colletotrichum gloeosporioides bacterial block with the diameter of 7mm, inoculating the bacterial block to the center of a PDA (personal digital assistant) plate, placing a filter paper sheet with the diameter of 7mm at a position 2cm away from the upper part and the lower part of the bacterial block, taking carbendazim, chlorothalonil and myclobutanil as positive controls and taking a banana colletotrichum gloeosporioides plate without inoculating actinomycetes as a control group, repeating each treatment for 3 times, and performing inversion culture at the constant temperature of 28 ℃ for 10 days; the bacteriostatic rate is shown in the following table 5, and the bacteriostatic effect is shown in fig. 5.
TABLE 5 photostability of the fermented extract of strain ITBB-ZK-a5 against phytopathogens
As can be seen from the data in Table 5, the bacterial strain ITBB-ZK-a5 fermentation extract has a downward trend of bacteriostasis rate along with the extension of ultraviolet illumination time, but still has a good bacteriostasis effect, the bacteriostasis rate is about 58-70%, is equivalent to the bacteriostasis activity of myclobutanil, and is stronger than the bacteriostasis activity of chlorothalonil. Therefore, the strain ITBB-ZK-a5 can play a stable role in preventing and treating diseases caused by the fungi and bacteria.
It should be noted that the above-mentioned embodiments are only illustrative and not restrictive, and any modifications or changes within the meaning and range of equivalents to the technical solutions of the present invention by those skilled in the art should be considered to be included in the protection scope of the present invention.
Sequence listing
<110> research institute of tropical biotechnology of Chinese tropical academy of agricultural sciences
<120> sponge-derived biocontrol streptomycete ITBB-ZK-a5 and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1517
<212> DNA
<213> Streptomyces biocontrol (Streptomyces sp)
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aaaggaggtg atccagccgc accttccggt acggctacct tgttacgact tcgtcccaat 60
cgccagtccc accttcgacg gctccctcca caagggttgg gccaccggct tcgggtgtta 120
ccgactttcg tgacgtgacg ggcggtgtgt acaaggcccg ggaacgtatt caccgcagca 180
atgctgatct gcgattacta gcgactccga cttcatgggg tcgagttgca gaccccaatc 240
cgaactgaga ccggcttttt gagattcgct ccacctcacg gcttcgcagc tcattgtacc 300
ggccattgta gcacgtgtgc agcccaagac ataaggggca tgatgacttg acgtcgtccc 360
caccttcctc cgagttgacc ccggcagtct cctgtgagtc cccggcataa cccgctggca 420
acacaggaca agggttgcgc tcgttgcggg acttaaccca acatctcacg acacgagctg 480
acgacagcca tgcaccacct gtacaccgac cacaaggggg cacccatctc tggatgtttc 540
cggtgtatgt caagccttgg taaggttctt cgcgttgcgt cgaattaagc cacatgctcc 600
gccgcttgtg cgggcccccg tcaattcctt tgagttttag ccttgcggcc gtactcccca 660
ggcggggaac ttaatgcgtt agctgcggca cggacaacgt ggaatgtcgc ccacacctag 720
ttcccaacgt ttacggcgtg gactaccagg gtatctaatc ctgttcgctc cccacgcttt 780
cgctcctcag cgtcagtatc ggcccagaga tccgccttcg ccaccggtgt tcctcctgat 840
atctgcgcat ttcaccgcta caccaggaat tccgatctcc cctaccgaac tctagcctgc 900
ccgtatcgaa tgcagacccg gagttaagcc ccgggctttc acatccgacg cgacaagccg 960
cctacgagct ctttacgccc aataattccg gacaacgctt gcgccctacg tattaccgcg 1020
gctgctggca cgtagttagc cggcgcttct tctgcaggta ccgtcactca cgcttcttcc 1080
ctgctgaaag aggtttacaa cccgaaggcc gtcatccctc acgcggcgtc gctgcatcag 1140
gcttgcgccc attgtgcaat attccccact gctgcctccc gtaggagtct gggccgtgtc 1200
tcagtcccag tgtggccggt cgccctctca ggccggctac ccgtcgtcgc cttggtaggc 1260
catcacccca ccaacaagct gataggccgc gggctcatcc tgcaccgccg gagctttcca 1320
cacgtatccc atgcgggaac gcgtcatatc cggtattaga ccccgtttcc agggcttgtc 1380
ccagagtgca gggcagattg cccacgtgtt actcacccgt tcgccactaa tccccggccg 1440
aaaccggttc atcgttcgac ttgcatgtgt taagcacgcc gccagcgttc gtcctgagcc 1500
aggatcaaac tctccgt 1517
Claims (5)
1. A sponge-derived Streptomyces biocontrol strain (Streptomyces sp.) ITBB-ZK-a5 is characterized in that: the preservation number is GDMCC No: 62383 it has a preservation date of 2022, 14/04, and the preservation unit is Guangdong province microorganism strain preservation center, and the preservation address is Guangdong province, Guangzhou city, Vibrio junior 100 college No. 59 building No. 5 building, Guangdong province academy of sciences microbiology.
2. A biocontrol product for controlling diseases in crops and/or aquaculture animals, characterized in that it comprises the fermentation extract of Streptomyces spongiensis (Streptomyces sp.) ITBB-ZK-a5 of claim 1 and/or Streptomyces spongiensis (Streptomyces sp.) ITBB-ZK-a5 of claim 1.
3. Use of the sponge-derived Streptomyces biocontrol (Streptomyces sp.) ITBB-ZK-a5 or the biocontrol product of claim 2 in the preparation of a medicament having an effect of controlling pathogenic fungi and/or pathogenic bacteria.
4. Use according to claim 3, characterized in that: the pathogenic fungi comprise one or more of banana wilt pathogen, No. 4 physiological race of banana wilt pathogen, banana colletotrichum, papaya brown calyx rot pathogen, papaya canker pathogen, mango colletotrichum, pepper corynebacterium sporangium, cowpea gray spot pathogen, cowpea stem rot pathogen, date palm pestalotiopsis, coconut fruit rot pathogen, rice blast pathogen, wheat gibberellic disease, cotton verticillium wilt pathogen, rubber colletotrichum, cucumber root rot pathogen, dragon fruit rot pathogen, Peronospora litchi pathogen and dragon boat flower colletotrichum.
5. Use according to claim 3, characterized in that: the pathogenic bacteria comprise one or more of rice bacterial blight, bacterial wilt eggplant, Vibrio harveyi, Streptococcus iniae and Streptococcus agalactiae.
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Cited By (1)
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| CN114933997A (en) * | 2022-06-13 | 2022-08-23 | 中国热带农业科学院热带生物技术研究所 | Coral-derived streptomyces symbiosis SH001 and application thereof |
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| CN114933997B (en) * | 2022-06-13 | 2023-06-23 | 中国热带农业科学院热带生物技术研究所 | Coral-derived symbiotic streptomyces SH001 and application thereof |
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