CN105409775A - Dendrobium officinale rooting and transplantation culture medium and culture method thereof - Google Patents
Dendrobium officinale rooting and transplantation culture medium and culture method thereof Download PDFInfo
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- CN105409775A CN105409775A CN201510874078.0A CN201510874078A CN105409775A CN 105409775 A CN105409775 A CN 105409775A CN 201510874078 A CN201510874078 A CN 201510874078A CN 105409775 A CN105409775 A CN 105409775A
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- dendrobium candidum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a dendrobium officinale rooting and transplantation culture medium which is formed by a basal culture medium and additives additionally added into the basal culture medium, wherein the pH value of the culture medium is 6.0-6.4; the addition amounts of the additive in the basal culture medium are respectively as follows: 1-3% of coconut milk, 1-3% of peeled potato, 3-5% of peeled banana, 3-5% of dry soybean, 0.05-0.10% of powdered activated carbon, 2-3% of saccharose, and 0.55-0.75% of agar. In the culture medium, natural additives are adopted for replacing traditional hormone, so that the problems of long adjustment time and high workload for the traditional culture medium additive hormone as well as poor stability and high aberration rate of cultured seedlings are effectively avoided. The preparation method of the culture medium is simple and convenient, easy to operate, wide in raw material source and low in cost; by using the culture medium for culture, the rooting and transplantation time is short, generally 11-12 weeks, the cultured seedlings are thick, steady in shape, excellent in quality and developed in roots, the survival rate of transplanting is not less than 99.2%, no aberrance occurs, and the planting efficiency, survival rate and quality of dendrobium officinale can be greatly improved.
Description
Technical field
The invention belongs to plant biotechnology field, be specifically related to a kind of dendrobium candidum strengthening seedling and rooting medium and preparation method and cultural method.
Background technology
Dendrobium candidum (
dendrobiumofficinale) be the orchid family Dendrobium herbaceos perennial, be listed in first of " Chinese nine immortal grass " in " Taoist Scriptures ", effects such as there is reinforcing yin essence benefit essence, nourishing the stomach to improve the production of body fluid, fall three height, improve body immunity, be anti-ageing, antitumor.Because it has unique effect, dendrobium candidum is since ancient times always by the scholar of vast health is favored.Dendrobium officinale is mainly distributed in the ground such as Zhejiang, Anhui, Guizhou, Jiangxi, Yunnan, Seeds of Dendrobium Candidum is because of the structure of its uniqueness, cannot sprout alone, only just likely naturally sprout when encountering suitable fungal component, in addition the madness of people to wild resource is excavated, and present dendrobium officinale resource is endangered.Along with the development of modern biotechnology tissue culture technology, the artificial breeding technology of current seedlings of Dendrobium officinale is formed gradually.
During traditional dendrobium candidum tissue is cultivated, strengthening seedling and rooting is generally by adding hormone as the growth of the promotion seedlings of Dendrobium officinale such as NAA, 6-BA and increment on the basis of basal medium.But the reasonable kind that hormone adds, addition are difficult to grasp, the many time and efforts of cost are often needed to carry out a large amount of basic research.The situations such as improper or excessive interpolation can cause plantlet in vitro poor growth, small and weak, yellowing leaf is mottled, overall chlorosis, aberration rate rising, in addition characterizing part often has performance hysteresis quality, and these unfavorable factors all have a strong impact on the High-efficient Production of seedling and the survival rate of field production.
Summary of the invention
First technical problem to be solved by this invention is to provide a kind of without hormone, without harmful chemical component interpolation, the dendrobium candidum strengthening seedling and rooting medium promoting strengthening seedling and rooting fast.
Second technical problem to be solved by this invention is to provide a kind of preparation method of workable dendrobium candidum strengthening seedling and rooting medium.
3rd technical problem to be solved by this invention is to provide a kind of method utilizing above-mentioned medium culture dendrobium candidum strong sprout.
The present invention solves the technical scheme that above-mentioned first technical problem adopt: a kind of dendrobium candidum strengthening seedling and rooting medium, this medium is made up of basal medium and the additive additionally made an addition in basal medium, the pH value of this medium is 6.0 ~ 6.4, with the weighing scale of described basal medium, the addition of described additive in described basal medium is respectively: coconut milk 1 ~ 3%, peeled potatoes 1 ~ 3%, peeling banana 3 ~ 5%, dry soybean 3 ~ 5%, powdered activated carbon 0.05 ~ 0.10%, sucrose 2 ~ 3%, agar 0.55 ~ 0.75%.
Described basal medium is 1/3 ~ 2/3MS basal medium.MS basal medium uses the most general medium at present, and its composition comprises macroelement, trace element, molysite, organic principle, inositol etc., can make by oneself or select commercially available prod.1/3MS basal medium and macroelement become the medium of original 1/3, and in like manner 2/3MS basal medium and macroelement become the medium of original 2/3.The application selects 1/3 ~ 2/3MS basal medium, can promote the growth of dendrobium candidum seedling better.
The present invention solves the technical scheme that above-mentioned second technical problem adopt: a kind of preparation method of dendrobium candidum strengthening seedling and rooting medium, comprises the following steps:
1) pretreatment of additive: squeezed the juice by fresh coconut and obtain coconut milk, by potato and banana peeling, sends out 8 ~ 12 hours by dry soybean in 20 ~ 28 DEG C of bubbly waters, and gets out powdered activated carbon, sucrose, agar, for subsequent use;
2) preparation of basal medium: preparation basal medium;
3) interpolation of additive: with the weighing scale of basal medium, take each additive, the addition of each additive in basal medium is respectively: coconut milk 1 ~ 3%, peeled potatoes 1 ~ 3%, peeling banana 3 ~ 5%, dry soybean 3 ~ 5%, powdered activated carbon 0.05 ~ 0.10%, sucrose 2 ~ 3%, agar 0.55 ~ 0.75%, by coconut milk, peeled potatoes, peeling banana, soybean, powdered activated carbon, sucrose and agar are added in basal medium successively, obtain dendrobium candidum strengthening seedling and rooting medium, by NaOH solution, the pH value of dendrobium candidum strengthening seedling and rooting medium is adjusted to 6.0 ~ 6.4,
4) packing and sterilizing: dendrobium candidum strengthening seedling and rooting medium is heated to 85 ~ 90 DEG C, packing, then sterilizing 20 ~ 25min in the high steam of 121 DEG C after insulation 5 ~ 8min, finally by for subsequent use for the cooling of the medium of bacterium of having gone out.
The present invention solves above-mentioned 3rd technical scheme that technical problem adopts: a kind of method utilizing above-mentioned medium culture dendrobium candidum strong sprout, incubation is: inoculation is highly the female seedling of dendrobium candidum of 1 ~ 1.5cm in the medium, illumination every day 11 ~ 13 hours at 24 ~ 28 DEG C, intensity of illumination is 2500 ~ 3000Lux, until strengthening seedling and rooting.
Compared with prior art, the invention has the advantages that: dendrobium candidum strengthening seedling and rooting medium of the present invention, natural additive is adopted to replace classical hormonal, add without harmful chemical component, effectively prevent conventional medium and add the seedling poor stability that hormone debug time is long, workload is large, turn out, the problem that aberration rate is high; The preparation method of dendrobium candidum strengthening seedling and rooting medium of the present invention is easy, easy to operate, and raw material sources are wide, cost is low; Utilize the dendrobium candidum strengthening seedling and rooting time of medium culture of the present invention short, general at 11 ~ 12 weeks, and the seedling seedling body cultivated is sturdy, dimensionally stable, best in quality, well developed root system, transplanting survival rate can reach more than 99.2%, occur without variation situation, therefore medium of the present invention significantly can improve efficiency, survival rate and the quality that dendrobium candidum is planted.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
The dendrobium candidum strengthening seedling and rooting medium of embodiment one, be made up of 1/3MS basal medium and the additive additionally made an addition in basal medium, the pH value of this medium is 6.0, with the weighing scale of 1/3MS basal medium, the addition of additive in 1/3MS basal medium is respectively: coconut milk 1%, peeled potatoes 1%, peeling banana 3%, dry soybean 3%, powdered activated carbon 0.05%, sucrose 2%, agar 0.55%, and the preparation method of this dendrobium candidum strengthening seedling and rooting medium comprises the following steps:
1) pretreatment of additive: squeezed the juice by fresh coconut and obtain coconut milk, by potato and banana peeling, sends out 10 hours by dry soybean in 25 DEG C of bubbly waters, and gets out powdered activated carbon, sucrose, agar, for subsequent use;
2) preparation of basal medium: preparation 1/3MS basal medium;
3) interpolation of additive: with the weighing scale of 1/3MS basal medium, take each additive and be added in 1/3MS basal medium successively, obtain dendrobium candidum strengthening seedling and rooting medium, by NaOH solution, the pH value of dendrobium candidum strengthening seedling and rooting medium is adjusted to 6.0;
4) packing and sterilizing: dendrobium candidum strengthening seedling and rooting medium is heated to 85 DEG C, packing, then sterilizing 20min in the high steam of 121 DEG C after insulation 5min, finally cool the medium of bacterium of having gone out, i.e. the medium of obtained embodiment one.
Utilize the method in medium culture dendrobium candidum strong sprout of embodiment one, incubation is: inoculation is highly the female seedling of dendrobium candidum of 1 ~ 1.5cm in the medium, illumination every day 13 hours at 24 DEG C, intensity of illumination is 2500Lux, record and obtain finished product seedling afterwards in 84 days, this seedling seedling body is sturdy, well developed root system, transplanting survival rate is 99.2%, and aberration rate is zero.
The dendrobium candidum strengthening seedling and rooting medium of embodiment two, be made up of 1/2MS basal medium and the additive additionally made an addition in basal medium, the pH value of this medium is 6.2, with the weighing scale of 1/2MS basal medium, the addition of additive in 1/2MS basal medium is respectively: coconut milk 2%, peeled potatoes 2%, peeling banana 4%, dry soybean 4%, powdered activated carbon 0.075%, sucrose 2.5%, agar 0.62%, and the preparation method of this dendrobium candidum strengthening seedling and rooting medium comprises the following steps:
1) pretreatment of additive: squeezed the juice by fresh coconut and obtain coconut milk, by potato and banana peeling, sends out 9 hours by dry soybean in 28 DEG C of bubbly waters, and gets out powdered activated carbon, sucrose, agar, for subsequent use;
2) preparation of basal medium: preparation 1/2MS basal medium;
3) interpolation of additive: with the weighing scale of 1/2MS basal medium, take each additive and be added in 1/2MS basal medium successively, obtain dendrobium candidum strengthening seedling and rooting medium, by NaOH solution, the pH value of dendrobium candidum strengthening seedling and rooting medium is adjusted to 6.2;
4) packing and sterilizing: dendrobium candidum strengthening seedling and rooting medium is heated to 88 DEG C, packing, then sterilizing 22min in the high steam of 121 DEG C after insulation 6.5min, finally cool the medium of bacterium of having gone out, i.e. the medium of obtained embodiment two.
Utilize the method in medium culture dendrobium candidum strong sprout of embodiment two, incubation is: inoculation is highly the female seedling of dendrobium candidum of 1 ~ 1.5cm in the medium, illumination every day 12 hours at 26 DEG C, intensity of illumination is 3000Lux, record and obtain finished product seedling afterwards in 80 days, this seedling seedling body is sturdy, well developed root system, transplanting survival rate is 99.5%, and aberration rate is zero.
The dendrobium candidum strengthening seedling and rooting medium of embodiment three, be made up of 2/3MS basal medium and the additive additionally made an addition in basal medium, the pH value of this medium is 6.4, with the weighing scale of 2/3MS basal medium, the addition of additive in 2/3MS basal medium is respectively: coconut milk 3%, peeled potatoes 3%, peeling banana 5%, dry soybean 5%, powdered activated carbon 0.10%, sucrose 3%, agar 0.75%, and the preparation method of this dendrobium candidum strengthening seedling and rooting medium comprises the following steps:
1) pretreatment of additive: squeezed the juice by fresh coconut and obtain coconut milk, by potato and banana peeling, sends out 11 hours by dry soybean in 22 DEG C of bubbly waters, and gets out powdered activated carbon, sucrose, agar, for subsequent use;
2) preparation of basal medium: preparation 2/3MS basal medium;
3) interpolation of additive: with the weighing scale of 2/3MS basal medium, take each additive and be added in 2/3MS basal medium successively, obtain dendrobium candidum strengthening seedling and rooting medium, by NaOH solution, the pH value of dendrobium candidum strengthening seedling and rooting medium is adjusted to 6.4;
4) packing and sterilizing: dendrobium candidum strengthening seedling and rooting medium is heated to 90 DEG C, packing, then sterilizing 25min in the high steam of 121 DEG C after insulation 8min, finally cool the medium of bacterium of having gone out, i.e. the medium of obtained embodiment three.
Utilize the method in medium culture dendrobium candidum strong sprout of embodiment three, incubation is: inoculation is highly the female seedling of dendrobium candidum of 1 ~ 1.5cm in the medium, illumination every day 11 hours at 28 DEG C, intensity of illumination is 3000Lux, record and obtain finished product seedling afterwards in 78 days, this seedling seedling body is sturdy, well developed root system, transplanting survival rate is 99.6%, and aberration rate is zero.
Claims (4)
1. a dendrobium candidum strengthening seedling and rooting medium, it is characterized in that, this medium is made up of basal medium and the additive additionally made an addition in basal medium, the pH value of this medium is 6.0 ~ 6.4, with the weighing scale of described basal medium, the addition of described additive in described basal medium is respectively: coconut milk 1 ~ 3%, peeled potatoes 1 ~ 3%, peeling banana 3 ~ 5%, dry soybean 3 ~ 5%, powdered activated carbon 0.05 ~ 0.10%, sucrose 2 ~ 3%, agar 0.55 ~ 0.75%.
2. a kind of dendrobium candidum strengthening seedling and rooting medium according to claim 1, is characterized in that described basal medium is 1/3 ~ 2/3MS basal medium.
3. a preparation method for the dendrobium candidum strengthening seedling and rooting medium described in claim 1 or 2, is characterized in that comprising the following steps:
1) pretreatment of additive: squeezed the juice by fresh coconut and obtain coconut milk, by potato and banana peeling, sends out 8 ~ 12 hours by dry soybean in 20 ~ 28 DEG C of bubbly waters, and gets out powdered activated carbon, sucrose, agar, for subsequent use;
2) preparation of basal medium: preparation basal medium;
3) interpolation of additive: with the weighing scale of basal medium, take each additive, the addition of each additive in basal medium is respectively: coconut milk 1 ~ 3%, peeled potatoes 1 ~ 3%, peeling banana 3 ~ 5%, dry soybean 3 ~ 5%, powdered activated carbon 0.05 ~ 0.10%, sucrose 2 ~ 3%, agar 0.55 ~ 0.75%, by coconut milk, peeled potatoes, peeling banana, soybean, powdered activated carbon, sucrose and agar are added in basal medium successively, obtain dendrobium candidum strengthening seedling and rooting medium, by NaOH solution, the pH value of dendrobium candidum strengthening seedling and rooting medium is adjusted to 6.0 ~ 6.4,
4) packing and sterilizing: dendrobium candidum strengthening seedling and rooting medium is heated to 85 ~ 90 DEG C, packing, then sterilizing 20 ~ 25min in the high steam of 121 DEG C after insulation 5 ~ 8min, finally by for subsequent use for the cooling of the medium of bacterium of having gone out.
4. one kind utilizes the method in the medium culture dendrobium candidum strong sprout described in claim 1 or 2, it is characterized in that incubation is: inoculation is highly the female seedling of dendrobium candidum of 1 ~ 1.5cm in the medium, illumination every day 11 ~ 13 hours at 24 ~ 28 DEG C, intensity of illumination is 2500 ~ 3000Lux, until strengthening seedling and rooting.
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Cited By (3)
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| CN106962165A (en) * | 2017-05-22 | 2017-07-21 | 东莞市睿绅生物技术有限公司 | A kind of preparation method of moveable dendrobium officinale culture medium and integrated method for culturing seedlings |
| CN107094627A (en) * | 2017-05-12 | 2017-08-29 | 江苏绿洲园艺绿化有限公司 | A kind of dendrobium candidum rooting of vitro seedling culture medium and preparation method thereof |
| CN109496781A (en) * | 2018-10-16 | 2019-03-22 | 皖西学院 | Dendrobidium huoshanness culture medium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107094627A (en) * | 2017-05-12 | 2017-08-29 | 江苏绿洲园艺绿化有限公司 | A kind of dendrobium candidum rooting of vitro seedling culture medium and preparation method thereof |
| CN106962165A (en) * | 2017-05-22 | 2017-07-21 | 东莞市睿绅生物技术有限公司 | A kind of preparation method of moveable dendrobium officinale culture medium and integrated method for culturing seedlings |
| CN109496781A (en) * | 2018-10-16 | 2019-03-22 | 皖西学院 | Dendrobidium huoshanness culture medium |
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