CN105396541B - A kind of albumen and sugared graft reaction equipment and the preparation method of glycosylation albumen - Google Patents
A kind of albumen and sugared graft reaction equipment and the preparation method of glycosylation albumen Download PDFInfo
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Abstract
The invention belongs to albumen deep process technology field, and in particular to a kind of albumen and sugared graft reaction equipment and the preparation method of glycosylation albumen.When carrying out the graft reaction of albumen and sugar using the equipment, reaction substrate is from feeding assembly enters reaction chamber, pH control assemblies, temperature-controlling module, pressure control assembly and mixing control component set regulation reaction condition jointly, after the completion of reaction, reaction product obtains target glycosylation albumen from discharge component exits into after-treatment device, after processing.The equipment provided using the present invention carries out graft reaction, improves the grafting degree of albumen and sugared graft reaction, improves emulsibility and dissolubility of the albumen after glycosylation, solves albumen and the sugared graft reaction time is long, low production efficiency reacts discontinuous problem.
Description
Technical field
The invention belongs to albumen deep process technology field, and in particular to a kind of albumen and sugared graft reaction equipment and glycosylation
The preparation method of albumen.
Background technology
Glycosylation modification is free amino group based on amino acid side chain in protein molecule and the carbonyl of glycan molecule reduction end
Carbonyl ammonia reaction-Maillard reaction between base, it is not necessary to which any chemical reagent can make the reaction as catalyst, only heating
Spontaneously carry out.It is mainly used in soybean protein, rice protein, lactalbumin, casein etc..Come from the document reported at present
See, the method that protein carries out graft modification with sugar mainly has two kinds of a dry heating method and wet heating, but the reaction of both approaches
Time is longer, particularly dry process reaction, at least tens of hours, at most several days or even several weeks, is unfavorable for application, and substitution value is low.
Wet heating adds the collision probability of albumen and sugar, shortens the time than dry heating method, but be mainly used in the reaction of small molecular sugar.Cause
It is short that this invents a kind of working time, it is adaptable to the graft reaction equipment of the sugar grafting of protein and various molecular weights, high to obtaining
Emulsibility and dissolubility albumen are most important.
The content of the invention
In order to solve the above-mentioned technical problem, the invention provides a kind of albumen and sugared graft reaction equipment and glycosylation albumen
Preparation method, improve the grafting degree of albumen and sugared graft reaction, improve emulsibility and dissolving of the albumen after glycosylation
Property, solve albumen and the sugared graft reaction time is long, low production efficiency, react discontinuous problem.
The present invention is achieved in that according to an aspect of the invention, there is provided a kind of albumen is set with sugared graft reaction
It is standby, including：
Feeding assembly, by albumen feed pipe, sugared feed pipe, distilled water feed pipe, charging modulator and charging control display
Device is constituted, and charging control display is used for setting the inventory needed for reaction, and the inventory information transmission of setting is adjusted to charging
Device is controlled, the inlet amount of each material in the albumen feed pipe, sugared feed pipe, distilled water feed pipe is controlled by feed controller；
Reaction chamber, albumen feed pipe, sugared feed pipe, distilled water feed pipe with feeding assembly are connected；
PH control assemblies, the pH of reactant in regulation and control reaction chamber are connected with reaction chamber, by pH detectors, alkali feed pipe, acid
Feed pipe, pH modulators and pH control display compositions, pH control displays are used for setting the pH needed for reaction, by the pH of setting
Information transmission gives pH modulators, and pH modulators are by the mixed material pH that detects the pH detectors stretched into reaction chamber with setting
Fixed pH compares, thus regulate and control from alkali feed pipe be material plus alkali or from sour feed pipe be material acid adding；
Temperature-controlling module, the temperature of reactant in regulation and control reaction chamber is connected with reaction chamber, is added by infrared radiation thermometer, microwave
Hot component, circulating water component, temperature regulator, temperature control display composition, temperature control display are used for setting anti-
Required temperature is answered, the temperature information of setting is sent to temperature regulator, temperature regulator is by the way that infrared radiation thermometer is measured
Reaction chamber in mixed material temperature with set reaction temperature compared so that regulate and control microwave heating component for material heat or adjust
Circulating water component is controlled for material to cool；Microwave heating component includes microwave generating apparatus, microwave absorbing device and microwave work(
Rate controls display, and microwave power control display is used for setting microwave power needed for reaction, power needed for reaction is sent to
Microwave generating apparatus, microwave generating apparatus is connected with reaction chamber, and microwave is launched into reaction chamber, and microwave absorbing device is located at reaction
The side wall of chamber and bottom surface, absorb the microwave produced；
Pressure control assembly, the pressure of reactant in regulation and control reaction chamber is connected with reaction chamber, by pressure gauge, snorkel, is taken out
Tracheae, pressure regulator and Stress control display composition, Stress control display are used for setting the pressure needed for reaction, will set
Fixed pressure information sends pressure regulator to, and pressure regulator passes through in the reaction chamber that measures the pressure gauge in reaction chamber
Pressure with set reaction pressure compared so that regulate and control snorkel for reaction chamber supply gas supercharging or exhaust tube from reaction chamber pumping drop
Pressure；
Mixing control component, is made up of, agitator is arranged on reaction agitator, stirring modulator and mixing control display
Intracavitary, mixing control display is used for setting the speed of agitator needed for reaction, by the speed of agitator information transmission of setting to stirring
Modulator, the rotating speed of stirring modulator control agitator is so as to realize the stirring of mixed material in reaction chamber；
Discharge component, including discharging opening, discharge nozzle, circulating pump and discharging control display, discharging opening are arranged on reaction chamber
Bottom, discharging control display is used for setting the reaction deadline, after the completion of reaction, and discharging control display control is opened out
Material mouth, circulating pump extracts reaction product out from the reaction chamber；And
After-treatment device, is connected with discharge nozzle, is pellicle dialysis apparatus.
Further, circulating water component includes flow solenoid valve, heat exchanger tube and refrigerating plant, and recirculated water is filled in refrigeration
Put to enter after refrigeration and occur heat exchange reduction reaction temperature in heat exchanger tube with reaction chamber, flow solenoid valve is by temperature regulator
Regulation and control control recirculated water uninterrupted.
According to another aspect of the invention, there is provided utilize above-mentioned graft reaction equipment to carry out albumen and sugared graft reaction
Method, comprise the following steps：
1) amount of albumen and distilled water needed for graft reaction being set on charging control display；
2) pH needed for setting prepares protein liquid on pH control displays, setting prepares albumen on mixing control display
Speed of agitator needed for liquid；
3) start the graft reaction equipment, protein liquid, halt device is made；
4) amount sugared needed for setting graft reaction on charging control display, controls display, temperature control to show in pH
Show to set on device, microwave power control display, Stress control display, mixing control display and discharging control display and connect
PH, temperature, microwave power, pressure, speed of agitator and time needed for branch reaction, equipment is again started up, carries out graft reaction；
5) after reaction terminates, the reaction cavity pressure is discharged by Stress control display, it is aobvious by temperature control
Show that device sets reaction cavity temperature to be room temperature, controlled by pH after display adjusts product pH again, it is aobvious using discharging control
Show that device opens discharging opening, reaction product is pumped in dialysis apparatus and dialysed by circulating pump, obtain graft reaction product glycosylation egg
In vain, it is freeze-dried.
Further, albumen is rice protein in the above method, and rice protein amount needed for reaction is 10-50g, amount of distilled water
For 1000-2000mL, pH needed for preparing protein liquid is 12, and speed of agitator is 300-700r/min, and the time is 30min；
Sugar is one kind in glucan, maltodextrin, glucose, lactose or soluble starch, and sugar amount needed for reaction is 15-
60g；
PH needed for graft reaction is 10.5, and temperature is 80-110 DEG C, and microwave power is 100-800W, and pressure is 40-
100MPa, speed of agitator is 300-700r/min, and the reaction time is 10-25min；
The pH of reaction product is 7.0.
Compared with prior art, the advantage of the invention is that：
1st, the albumen that the present invention is provided overcomes albumen physical modification, chemical modification and enzyme process with sugared graft reaction equipment and changed
Property the time length, the shortcomings of react slow, it is long to solve the reaction time existed using traditional graft reaction, the problem of cost is higher；
2nd, the equipment that albumen is provided with sugar using the present invention carries out graft reaction, and grafting degree is greatly improved, and graft
Emulsibility and dissolubility are greatly improved, and obtained protein grafting sugar can be used for field of food.
Brief description of the drawings
Below in conjunction with the accompanying drawings and embodiment the present invention is further detailed explanation：
Albumen and sugared graft reaction device structure schematic diagram that Fig. 1 provides for the present invention；
The equipment modules connection diagram that Fig. 2 provides for the present invention；
Fig. 3 is glycosylation rice protein Fourier's infared spectrum that embodiment 1-5 is obtained.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with the accompanying drawings and embodiment, it is right
The present invention is further elaborated.It should be appreciated that specific embodiment described herein is used only for explaining the present invention, and
It is not used in the restriction present invention.
In order to solve existing dry method, wet method prepare glycosylation the albumen time length, efficiency it is low, obtained graft product grafting degree
The problem of low, emulsibility and not high dissolubility, the invention provides a kind of albumen and sugared graft reaction equipment and utilize the equipment
The method for carrying out graft reaction, the equipment can make albumen with sugared graft reaction in constant-temperature high-pressure and the common work of microwave radiation
Carried out under, accelerate reaction rate and reaction process, improve the grafting degree of product, make modified protein solubility, emulsification
Property is improved.
With reference to Fig. 1, the albumen that the present invention is provided and sugared graft reaction equipment, including feeding assembly 1, reaction chamber 2, pH control
Component 3, temperature-controlling module 4, pressure control assembly 5, mixing control component 6, discharge component 7 and after-treatment device 8 are constituted, and are entered
Expect component 1 by albumen feed pipe 11, distilled water feed pipe 12, sugared feed pipe 13, charging modulator 14 and charging control display
15 compositions, charging control display 15 is used for setting the inventory needed for reaction, by the inventory information transmission of setting to charging
Modulator 14, albumen feed pipe 11, sugared feed pipe 13, the distilled water feed pipe into reaction chamber 2 are controlled by feed controller 14
The inlet amount of each material in 12；
PH control assemblies 3 are shown by pH detectors 31, alkali feed pipe 32, sour feed pipe 33, pH modulators 34 and pH controls
Device 35 is constituted, and pH control displays 35 are used for setting the pH needed for reaction, by the pH information transmissions of setting to pH modulators 34, pH
Modulator 34 is compared by the mixed material pH for detecting pH detectors 31 with the pH set, so as to regulate and control from alkali feed pipe 32
For material in reactor 2 add alkali or from sour feed pipe 33 be reactor 2 in material acid adding；
Temperature-controlling module 4 is by infrared radiation thermometer 41, microwave heating component 42, circulating water component 43, temperature adjusting
Device 44 and temperature control display 45 are constituted, and temperature control display 45 is used for setting the temperature needed for reaction, by the temperature of setting
Information transmission is spent to temperature regulator 44, and temperature regulator 44 passes through mixture in the reaction chamber 2 that measures infrared radiation thermometer 41
Material temperature degree is compared with the reaction temperature set, is followed so as to regulate and control microwave heating component 42 for material heating or regulation and control in reaction chamber 2
Ring water cooled components 43 are material cooling in reaction chamber 2；
Pressure control assembly 5 is shown by pressure gauge 51, snorkel 52, exhaust tube 53, pressure regulator 54 and Stress control
Device 55 is constituted, and Stress control display 55 is used for setting the pressure needed for reaction, and sending the pressure information of setting to pressure adjusts
Device 54 is controlled, pressure regulator 54 is compared by the pressure in the reaction chamber 2 that measures pressure gauge 51 with the reaction pressure of setting, from
And regulate and control snorkel 52 and supply gas supercharging or exhaust tube 53 from the pumped down of reaction chamber 2 for reaction chamber 2；
Mixing control component 6 is made up of agitator 61, stirring modulator 62 and mixing control display 63, and mixing control shows
Show that device 63 is used for setting the speed of agitator needed for reaction, stirring modulator 62, stirring are given by the speed of agitator information transmission of setting
The rotating speed of the control agitator 61 of modulator 62 is so as to realize the stirring of mixed material in reaction chamber 2；
Discharge component 7 includes discharging opening 71, discharge nozzle 72, circulating pump 73 and discharging control display 74, and discharging control is aobvious
Show that device 74 is used for setting the reaction deadline, after the completion of reaction, discharging opening 71, circulation are opened in the discharging control control of display 74
Pump 73 extracts reaction product out from reaction chamber 2；
After-treatment device 8 is connected with discharge nozzle 72, is pellicle dialysis apparatus.
Microwave heating component 42 includes microwave generating apparatus 421, microwave absorbing device 422 and microwave power control display
423, microwave power control display 423 is used for setting microwave power needed for reaction, sends power needed for reaction to microwave hair
Generating apparatus 421, microwave generating apparatus 421 is connected with reaction chamber 2, and microwave is launched into reaction chamber 2, and microwave absorbing device 422 is set
In the side wall of reaction chamber 2 and bottom surface, the microwave produced is absorbed.
Circulating water component 43 includes flow solenoid valve 431, heat exchanger tube 432 and refrigerating plant 433, and recirculated water is in refrigeration
Entered after freezing in device 433 and occur heat exchange reduction reaction temperature, flow solenoid valve 431 in heat exchanger tube 432 with reaction chamber 2
By the uninterrupted of the regulation and control control recirculated water of temperature regulator 44.
During working condition, feeding assembly 1 is connected respectively at each charge holder；The soda acid feed pipe of pH control assemblies 3
It is connected respectively with soda acid reservoir；The snorkel 52 of pressure control assembly 5 is connected with water vapor generation device, it is necessary to increase reaction
During pressure in chamber 2, completed by sending into vapor, exhaust tube 53 is connected with aspiration pump.
Civil power provides power supply for this equipment, with reference to Fig. 2, and each control display is in parallel, each control display and its institute
The downstream module series connection of control, completes the control of whole equipment.
When carrying out the graft reaction of albumen and sugar using the equipment, reaction substrate enters reaction chamber 2 from feeding assembly 1
In, pH control assemblies 3, temperature-controlling module 4, pressure control assembly 5 and mixing control component 6 set regulation reaction bar jointly
Part, after the completion of reaction, reaction product is exited into after-treatment device 8 from discharge component 7, and target glycosylation is obtained after processing
Albumen.
Embodiment 1
1) amount of rice protein is 10g needed for charging controls to set graft reaction on display, and the amount of distilled water is set to
1000mL；
2) control pH needed for setting prepares protein liquid on display to be 12 in pH, set and prepare on mixing control display
Speed of agitator needed for protein liquid is 300r/min；
3) the graft reaction equipment is started, the reaction time is 30min, and halt device after protein liquid is made；
4) control amount sugared needed for setting graft reaction on display to be 30g in charging, display, temperature control are controlled in pH
On on display processed, microwave power control display, Stress control display, mixing control display and discharging control display
PH needed for graft reaction is set be 10.5, temperature be 110 DEG C, microwave power be that 100W, pressure are that 100MPa, speed of agitator are
300r/min, the reaction time is 10min, is again started up equipment, carries out graft reaction；
5) after reaction terminates, the reaction cavity pressure is discharged by Stress control display, it is aobvious by temperature control
Show that device sets reaction cavity temperature to be room temperature, it is 7.0 to control display to adjust product pH again by pH, aobvious using discharging control
Show that device opens discharging opening, reaction product is pumped in dialysis apparatus the 24h that dialyses, obtains graft reaction product glucose by circulating pump
Glycosylate rice protein, freeze-drying.
Embodiment 2
The places different from embodiment 1 are：
Step 1) in rice protein addition be set to 20g, amount of distilled water is set to 1500mL, and the speed of agitator of protein liquid is made
For 400r/min；
Step 4) in the sugar that adds be maltodextrin, addition is set to 60g, and reaction temperature is 100 DEG C, and microwave power is
300W, pressure is 80MPa, and speed of agitator is 400r/min, and the reaction time is 15min.
Embodiment 3
The places different from embodiment 1 are：
Step 1) in rice protein addition be set to 10g, amount of distilled water is set to 1500mL, and the speed of agitator of protein liquid is made
For 500r/min；
Step 4) in the sugar that adds be glucose, addition is set to 20g, and reaction temperature is 90 DEG C, and microwave power is 500W,
Pressure is 60MPa, and speed of agitator is 500r/min, and the reaction time is 25min.
Embodiment 4
Step 1) in rice protein addition be set to 20g, amount of distilled water is set to 1500mL, and the speed of agitator of protein liquid is made
For 600r/min；
Step 4) in the sugar that adds be maltodextrin, addition is set to 60g, and reaction temperature is 100 DEG C, and microwave power is
300W, pressure is 80MPa, and speed of agitator is 600r/min, and the reaction time is 15min.
Embodiment 5
Step 1) in rice protein addition be set to 50g, amount of distilled water is set to 2000mL, and the speed of agitator of protein liquid is made
For 700r/min；
Step 4) in the sugar that adds be lactose, addition is set to 50g, and reaction temperature is 80 DEG C, and microwave power is 800W, pressure
Power is 40MPa, and speed of agitator is 700r/min, and the reaction time is 15min.
To connecing for the glycosylation rice protein that is obtained in embodiment 1, embodiment 2, embodiment 3, embodiment 4 and embodiment 5
Branch degree, pH are that 7 temperature are that solubility, emulsibility at 25 DEG C is detected,
The detection method of grafting degree is as follows：
The method for determining free amino group using OPA methods determines the grafting degree of glycosylation albumen.Specific method is as follows：It is accurate to claim
Take 40mg OPA to be dissolved in lmL methanol, be separately added into 20% (w/v) SDS 2.5mL, 0.lmol/L borax 25mL and
The coloured glaze base ethanol of 100uL β mono-, finally with distilled water constant volume to 50ml, this is OPA reagents, and is prepared immediately using preceding.During measure,
OPA reagents 4mL is measured in test tube, injection 200uL sample liquids (5mg/mL) mix and react 2min after 35 DEG C, under 340nm
Survey its light absorption value A340, to add 200uL water in OPA reagents as blank.Standard curve is made with lysine, calculated according to Δ A
The content of free amine group in sample, grafting degree (DG) calculation formula：
DG=(C0-Ct)/C0× 100%
Wherein：C0：Free amino groups of protein total amount during for unreacted；
Ct：To react the content of t free amino groups of protein
Deliquescent detection method is as follows：
Take 0.5g protein samples to be scattered in 20mL distilled water, 2h is stirred on magnetic stirring apparatus, be transferred to 25mL capacity
Constant volume in bottle, takes 9mL in 10mL centrifuge tubes, 7000rpm centrifugation 10min, accurate Aspirate supernatant 0.1mL, according to
Bradford methods are determined, and standard curve is done using bovine serum albumin (BSA), light absorption value at 595nm are determined, according to albumen in sample
Protein content calculates dissolubility in content and solution, is represented (NSI%) with nitrogen soluble index.
The detection method of emulsibility is as follows：
Using nephelometry Pearce et al. (1978), comprise the following steps that：Prepare 0.1% (m/v) sample solution
25mL is in pH7.0,0.1mol/L phosphate buffer solution.25mL sample solutions are taken to add 8mL soybean oils, at room temperature in height
10000rpm homogeneous 1min in fast refiner, forms emulsion.After homogeneous terminates, drawn respectively in 0min and 10min from bottom
100 μ L samples, add 10mL 0.1%SDS (m/v) dilutions, and absorbance (determining three times), emulsifying activity are determined at 500nm
Index (Emulsification Activity Index, EAI) is calculated as follows:
In formula：
EAI(m2/g)：N extension ratesFraction shared by oil phase in system
C：Protein concentration (g/ml)
L：It is colorimetric pool optical path (1cm)
Emulsion stability (Emulsification Stability Index, ESI) is calculated as follows:
ESI=(A0×ΔT)/(A0-A10)
In formula：
ESI：Stable emulsifying sex index, min；
A0：Absorbance during 0min；
ΔT：The time interval twice of emulsibility is determined, this experiment takes 10min；
A10：Absorbance during 10min, testing result is as shown in the table：
Table 1, embodiment product detection result
It was found from upper table result：Under the microwave and condition of high voltage of the equipment, rice protein and sugared graft reaction time subtract
As little as 10~20min, grafting degree is up to 21~31%, while the dissolubility of graft is up to 63.58%~83.58%, than not
Modified rice protein highest can improve 10%~56.6%, and emulsibility reaches as high as 22.16, than unmodified rice protein
31.8%~48.1% is improved, emulsion stability reaches as high as 51.51, carried than unmodified rice protein and improve 24.1%
~62.2%, reach the purpose for accelerating the sugared graft reaction time and changing rice protein dissolubility and emulsibility.
Fourier's infrared detection：
It is the glycosylation rice to being obtained in embodiment 1, embodiment 2, embodiment 3, embodiment 4 and embodiment 5 with reference to Fig. 3
Albumen carries out the collection of illustrative plates that Fourier's infrared scan is obtained, it can be seen that using FTIR spectrum method, to graft FT-
IR spectrum are analyzed, it can be seen that both had the phthalein amines of albumen with the infrared spectrum of the rice protein after sugar grafting
Absorption band characteristic peak, again have sugar characteristic absorption peak, combination product near 3350cm-1 exist by O-H and N-H shapes
Into wider absorption band, this be due to novel substance covalent cross-linking reaction occur in that new N-H keys, at 1651cm-1 (acid amides I)
There is strong C=O stretching vibration at place, illustrates that group is reacted herein, and this vibration can form β-corner, 1539 (acid amides
II) there is N-H change angular oscillation in N-H keys stretching vibration enhancing, and it belongs to beta sheet, is made up of the polypeptide chain stretched,
1153cm-1 (acid amides III) place peak belongs to N-H and becomes alpha-helix and random coil that angular oscillation and C-N stretching vibrations are formed；Together
When stronger absorption is occurred in that in 1020cm-1, this is probably the O-H flexural vibrations occurred due to the access of hydroxyl, by right
The analysis of product infared spectrum, it can be concluded that rice protein structure can be changed by the equipment and make rice protein with
Sugar there occurs graft reaction, and graft copolymer is generated by covalent bond.
Claims (2)
1. a kind of method that albumen and sugared graft reaction are carried out using graft reaction equipment, it is characterised in that the graft reaction
Equipment includes：
Feeding assembly, by albumen feed pipe, sugared feed pipe, distilled water feed pipe, charging modulator and charging control display group
Into, charging control display is used for setting the inventory needed for reaction, and charging modulator is given by the inventory information transmission of setting,
The inlet amount of each material in the albumen feed pipe, sugared feed pipe, distilled water feed pipe is controlled by charging modulator；
Reaction chamber, albumen feed pipe, sugared feed pipe, distilled water feed pipe with the feeding assembly are connected；
PH control assemblies, are connected the pH of reactant in the reaction chamber with the reaction chamber, are fed by pH detectors, alkali
Pipe, sour feed pipe, pH modulators and pH control display compositions, pH control displays are used for setting the pH needed for reaction, will set
Fixed pH information transmissions give pH modulators, and pH modulators pass through the mixing that detects the pH detectors stretched into the reaction chamber
Material pH with set pH compared so that regulate and control from the alkali feed pipe be material plus alkali or from sour feed pipe be material acid adding；
Temperature-controlling module, is connected the temperature of reactant in the reaction chamber with the reaction chamber, by infrared radiation thermometer, micro-
Wave heating component, circulating water component, temperature regulator, temperature control display composition, temperature control display are used for setting
Temperature needed for fixed reaction, sends the temperature information of setting to temperature regulator, and temperature regulator is by by infrared radiation thermometer
Mixed material temperature is compared with the reaction temperature set in the reaction chamber measured, is heated so as to regulate and control microwave heating component for material
Or regulation and control circulating water component cools for material；The microwave heating component includes microwave generating apparatus, microwave absorbing device
Display is controlled with microwave power, microwave power control display is used for setting microwave power needed for reaction, by work(needed for reaction
Rate sends microwave generating apparatus to, and the microwave generating apparatus is connected with the reaction chamber, and microwave, microwave are launched into reaction chamber
Absorption plant is located at side wall and the bottom surface of the reaction chamber, absorbs the microwave produced；
Pressure control assembly, is connected the pressure of reactant in the reaction chamber with the reaction chamber, by pressure gauge, supplies gas
Pipe, exhaust tube, pressure regulator and Stress control display composition, Stress control display are used for setting the pressure needed for reaction
Power, sends the pressure information of setting to pressure regulator, and pressure regulator passes through the institute that measures the pressure gauge in reaction chamber
The pressure stated in reaction chamber is compared with the reaction pressure set, is pressurized or is evacuated so as to regulate and control the snorkel and be supplied gas for reaction chamber
Pipe is from reaction chamber pumped down；
Mixing control component, is made up of, agitator is arranged on the reaction agitator, stirring modulator and mixing control display
Intracavitary, mixing control display is used for setting the speed of agitator needed for reaction, by the speed of agitator information transmission of setting to stirring
Modulator, the rotating speed of stirring modulator control agitator is so as to realize the stirring of mixed material in the reaction chamber；
Discharge component, including discharging opening, discharge nozzle, circulating pump and discharging control display, discharging opening are arranged on the reaction chamber
Bottom, discharging control display is used for setting the reaction deadline, after the completion of reaction, and discharging control display control is opened out
Material mouth, circulating pump extracts reaction product out from the reaction chamber；And
After-treatment device, is connected with the discharge nozzle, is pellicle dialysis apparatus；
Methods described comprises the following steps：
1) amount of albumen and distilled water needed for graft reaction being set on charging control display；
2) pH needed for setting prepares protein liquid on pH control displays, setting prepares protein liquid institute on mixing control display
Need speed of agitator；
3) start the graft reaction equipment, protein liquid, halt device is made；
4) sugared amount needed for graft reaction being set on charging control display, pH control display, temperature control display,
Set grafting anti-on microwave power control display, Stress control display, mixing control display and discharging control display
PH, temperature, microwave power, pressure, speed of agitator and time needed for answering, equipment is again started up, carries out graft reaction；
5) after reaction terminates, the reaction cavity pressure is discharged by the Stress control display, passes through the temperature control
Display processed sets reaction cavity temperature to be room temperature, controls after display adjusts product pH again, to utilize discharging control by pH
Display processed opens discharging opening, and reaction product is pumped in dialysis apparatus and dialysed by the circulating pump, obtains graft reaction product
Glycosylate albumen, freeze-drying；
Wherein, the albumen is rice protein, and rice protein amount is 10-50g needed for reaction, and amount of distilled water is 1000-2000mL,
PH needed for preparing protein liquid is 12, and speed of agitator is 300-700r/min, and the time is 30min；
The sugar is one kind in glucan, maltodextrin, glucose, lactose or soluble starch, and sugar amount needed for reaction is 15-
60g；
PH needed for graft reaction is 10.5, and temperature is 80-110 DEG C, and microwave power is 100-800W, and pressure is 40-100MPa, is stirred
Mix rotating speed is 300-700r/min, and the reaction time is 10-25min；
The pH of reaction product is 7.0.
2. the method according to claim 1 that albumen and sugared graft reaction are carried out using graft reaction equipment, its feature is existed
In the circulating water component includes flow solenoid valve, heat exchanger tube and refrigerating plant, and recirculated water is made in the refrigerating plant
Entered after cold and occur heat exchange reduction reaction temperature in heat exchanger tube with reaction chamber, the flow solenoid valve is by the temperature adjusting
The uninterrupted of the regulation and control control recirculated water of device.
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| CN106420666A (en) * | 2016-11-25 | 2017-02-22 | 沈阳师范大学 | Preparation method and device of glycosylation zein nano-carrier |
| CN108003219A (en) * | 2017-12-12 | 2018-05-08 | 安徽省寿县丰茂农产品开发有限公司 | A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification to it |
| US10994255B2 (en) * | 2018-01-16 | 2021-05-04 | Alliance Bioenergy Plus Inc. | System to convert cellulosic materials into sugar and method of using the same |
| CN109730294A (en) * | 2019-03-12 | 2019-05-10 | 武汉商学院 | Embedding method and application based on glucan-soybean protein isolate graft dihydrochalcone-type sweetening agent |
| CN110074353A (en) * | 2019-06-04 | 2019-08-02 | 吉林大学 | A kind of preparation method of high grafting degree pectin egg white product |
| CN115261130B (en) * | 2022-09-27 | 2023-01-17 | 山东渤海油脂工业有限公司 | Production device for refining soybean oil by enzyme method |
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| CN1594401A (en) * | 2004-07-09 | 2005-03-16 | 江南大学 | Method for graft modification of soyabean protein-sugar |
| CN101381399A (en) * | 2008-10-09 | 2009-03-11 | 江南大学 | Microwave preparation method of rice protein glycosylation modification |
| CN101654478A (en) * | 2009-08-25 | 2010-02-24 | 华南理工大学 | Soyabean protein glycosylation graft modification method |
| CN101785522A (en) * | 2010-03-26 | 2010-07-28 | 华南理工大学 | Graft polymerization method of vegetable protein and polysaccharide |
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| CN1594401A (en) * | 2004-07-09 | 2005-03-16 | 江南大学 | Method for graft modification of soyabean protein-sugar |
| CN101381399A (en) * | 2008-10-09 | 2009-03-11 | 江南大学 | Microwave preparation method of rice protein glycosylation modification |
| CN101654478A (en) * | 2009-08-25 | 2010-02-24 | 华南理工大学 | Soyabean protein glycosylation graft modification method |
| CN101785522A (en) * | 2010-03-26 | 2010-07-28 | 华南理工大学 | Graft polymerization method of vegetable protein and polysaccharide |
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