CN105395434A

CN105395434A - Application of floridoside to cosmetics

Info

Publication number: CN105395434A
Application number: CN201510973664.0A
Authority: CN
Inventors: 严江燕; 陈海敏; 陈娟娟; 杨锐; 严小军
Original assignee: Ningbo University
Current assignee: Ningbo University
Priority date: 2015-12-21
Filing date: 2015-12-21
Publication date: 2016-03-16

Abstract

The invention provides application of floridoside to cosmetics. Floridoside serves as a cosmetic additive. The additive is a moisture retention agent, a moisture absorbing agent or a whitening agent. Floridoside in use has the advantage of being free of toxic, is high in use safety, has better moisture retention performance and has a unique function in the aspect of inhibition of activity of monophenolase and diphenolase of monophenolase so that floridoside can reduce melanin in skin, which can be proved in melanin inhibition experiments of floridoside on melanoma cells. The action mechanism is clear, activity inhibition performance is good, and floridoside can be used in the cosmetic field and can be added as a whitening component.

Description

The application of floridosides in cosmetics

Technical field

The invention belongs to marine active substance applied technical field, be specifically related to the application of a kind of floridosides in cosmetics.

Background technology

Moisture in the moist and elasticity of skin and skin has substantial connection, when the excessive loss of the moisture in skin, can cause xerosis cutis, follow the string, accelerated ageing.Therefore moisture absorption, moisturizing are one of effects of skin protection cosmetics always.Desirable wetting agent should possess good wettability power, and moisture-keeping functions is lasting and stablize.Whitening is then another effect of cosmetics, and determine that the principal element of skin color is the melanin in skin, the depth of the colour of skin depends mainly on the ability of melanocyte synthesis of melanin.The effect of melanic generation and tryrosinase, DOPA quinone tautomerase and 5,6-dihydroxy indole-2-carboxylic oxidase is relevant.Desirable whitening agent should be able to act on the melanocyte of Showed Very Brisk rapidly, enduringly, stops melanin to be formed, reach and remove excessively calm melanic object at too many levels.And find that the new material with whitening skin and preserving moisture effect is the emphasis of cosmetics research field always.

Summary of the invention

Technical problem to be solved by this invention is to provide the application of floridosides in cosmetics, thus makes up the deficiencies in the prior art.

First the present invention provides floridosides preparing the application in cosmetics;

Described application is the application as cosmetics additive;

Described additive is wetting agent, hygroscopic agent or whitening agent;

Described floridosides, its structural formula is as follows:

The floridosides used, its preparation method is as follows:

Red algae powder is placed in 70-80% ethanol, carry out lixiviate in 60-70 DEG C to go out, after extracting solution filters, centrifugal 10 minutes of 7000r/min, be extracted with ethyl acetate rear ion exchange column of crossing and carry out purification, gained colourless transparent solution uses anhydrous alcohol solution after rotating evaporate to dryness, finally alcoholic solution is rotated evaporate to dryness, obtains floridosides of the present invention.

Described red algae comprises porphyra haitanensis, Eucheuma gelatinosum, dulse or Thallus Gracilariae;

Another aspect of the present invention provides a kind of wetting agent, hygroscopic agent or whitening agent, is to use above-mentioned floridosides to prepare.

The floridosides that the present invention uses has nontoxic feature, safe to use, has more outstanding performance of keeping humidity; And the unique function had in its single phenolase at restraint of tyrosinase and o-diphenolase activity, enable this floridosides reduce melanic formation in skin, this point is confirmed from the melanin Inhibition test of floridosides to melanoma cell.Therefore its mechanism of action is clear and definite, and inhibit activities is good, can be used in cosmetic field, adds as whitening composition.

Accompanying drawing explanation

Fig. 1 floridosides is on the impact of tryrosinase diphenol enzyme activity;

Fig. 2 floridosides suppresses type to tryrosinase;

Fig. 3 floridosides suppresses the mensuration of constant to tryrosinase;

The fate map of Fig. 4 floridosides restraint of tyrosinase list phenolase reaction;

Fig. 5 floridosides is on the impact of tryrosinase list phenolase stable state enzyme activity;

Fig. 6 floridosides is to the effect diagram of the melanoma cell rate of increase;

The effect diagram of Fig. 7 floridosides B16-F0 melanoma cell synthesis of melanin.

Detailed description of the invention

Below in conjunction with drawings and Examples, the present invention is described in further detail.

Embodiment 1: prepare floridosides

Floridosides is prepared from Thallus Porphyrae:

Get 200g Thallus Porphyrae powder, 2000mL70% ethanol is in 3000mL triangular flask, and with the sealing of sealed membrane gauze, be placed in shaking bath, 70 DEG C of water-baths 6 hours, 800r/min takes out.Solution is used multilamellar filtered through gauze, filtering residue hot ethanol rinses several times, and gained solution filters, and is extracted by filtering residue.Solution rotating evaporated, surplus solution is aqueous solution, gets solution, centrifugal 10 minutes of 7000r/min.Gained solution with ethyl acetate (1:1) about 500mL extracts 3 times.

By cation exchange column ((Dowex500W × 8100-200 (H) ionexchangeresin, D1008) after being washed till neutrality fast with pure water, when the above water of resin will drain off, slowly add sample liquid, and try not resin face to break up.Anion-exchange column (Dowex1 × 8100-200 (Cl) ionexchangeresin, D1016) is washed till neutrality with pure water, adds sample, repeat above step.Gained colourless transparent solution rotary evaporation, to evaporate to dryness, pours heating in water bath after dehydrated alcohol into, ultrasonic some minutes in round-bottomed flask, collect alcoholic solution, repeatedly.Alcoholic solution after centrifugal is rotated evaporate to dryness, obtains floridosides.

Get the raw materials of dulse as floridosides, preparation method is the same.

Embodiment 2: moisture absorption effect of floridosides

Using glycerol as positive control, respectively under the humidity of 80%, 43% and 29%, investigate the moisture pick-up properties of floridosides prepared by embodiment 1.As seen from the table, under three kinds of relative humidities, the hydroscopicity of floridosides is respectively 29.51%, 6.23%, 21.83%.Under R.H.80%, floridosides every 2h hydroscopicity increases with about 2% amplitude.Can find out from hygroscopicity test results, floridosides has certain hygroscopicity.

Table 1: the moisture pick-up properties of floridosides under three kinds of humidity (%)

Embodiment 3: the moisture-keeping efficacy of floridosides

In this test example, at relative humidity R.H.80% and 43% time, positive control glycerol has good moisture retention, floridosides then shows stronger performance of keeping humidity, and can play moisture-keeping function at short notice, extends in time, maintain good moisturizing rate, and all higher than glycerol.Under relative humidity R.H.29%, floridosides moisture retention is consistent with glycerol.

The performance of keeping humidity of table 2 floridosides under three kinds of humidity (%)

Embodiment 4: the tryrosinase of floridosides suppresses effect

Tryrosinase is the key enzyme of B16 cell, and its catalytic tyrosine forms DOPA and DOPA quinone, finally produces melanin.Therefore, it is possible to suppress the material of this enzymatic activity, the effect of skin whitening can be reached.First in this example, from the residual activity of enzyme and the concentration curve (Fig. 1) of floridosides, 40mmol/L floridosides can make the stable state vigor of enzyme decline 33.08%.From floridosides, type (Fig. 2) is suppressed to tryrosinase, the inhibitory action of floridosides to tryrosinase two phenolase is reversible competitive inhibition, suppression constant is 0.02mmol/L (Fig. 3), illustrates that the diphenol enzyme activity of this material to tryrosinase has good inhibitory action.

In this test example, variable concentrations floridosides is to tryrosinase list phenolase vigor, and time from reaction, the formation volume of product just rises lentamente, linearly rises after certain hour, reaction system reaches constant response speed, illustrates that catalytic reaction has reached stable state (Fig. 4).The enzyme activity of stable state declines along with the increase of inhibitor concentration, illustrates that the single phenolase activity of floridosides to tryrosinase has certain inhibitory action.In order to determine the inhibition strength of this material to tryrosinase list phenolase, with enzyme stable state vigor mapping (Fig. 5).100mmol/L floridosides makes enzyme activity have dropped 57.15%, records the inhibitor concentration (IC of the stable state vigor decline 50% of enzyme ₅₀) be about 89mmol/L.

Embodiment 5: floridosides secretes melanic suppression to melanoma cell

First this test example observes the Proliferation Ability situation of floridosides to the cell of melanoma cell B10-F0.Result shows, and floridosides does not have obvious lethal effect (Fig. 6) to B16-F0 melanoma cells under 4mmol/L concentration, illustrates that such material is the compound to cytotoxic, safety.And within the scope of this safe concentration, floridosides but can be good at suppressing the melanin of B16-F0 cell to form (Fig. 7), and the change in concentration trends.When concentration is 3.2mmol/L, suppression ratio is 27.71%.Therefore, can confirm intuitively from cell results, floridosides can effectively melanic generation in T suppression cell, has the effect of whitening.

Claims

1. an application for floridosides, is characterized in that, the application in cosmetics is being prepared in described application.

2. apply as claimed in claim 1, it is characterized in that, described application is the application of floridosides as cosmetics additive.

3. apply as claimed in claim 2, it is characterized in that, described additive is wetting agent, hygroscopic agent or whitening agent.

4. apply as claimed in claim 1 or 2, it is characterized in that, described floridosides, its structural formula is as follows:

5. apply as claimed in claim 1 or 2, it is characterized in that, described floridosides, its preparation method is as follows:

6. apply as claimed in claim 5, it is characterized in that, described red algae is porphyra haitanensis, Eucheuma gelatinosum, dulse or Thallus Gracilariae.

7. a cosmetic humectant, is characterized in that, employs floridosides in described wetting agent.

8. a hygroscopic agent, is characterized in that, employs floridosides in described hygroscopic agent.

9. a whitening agent, is characterized in that, employs floridosides in described whitening agent.