CN105388057A - Disposable dropping dye liquor for small samples in gastric mucosa biopsy and interventional operations - Google Patents
Disposable dropping dye liquor for small samples in gastric mucosa biopsy and interventional operations Download PDFInfo
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- CN105388057A CN105388057A CN201510990333.8A CN201510990333A CN105388057A CN 105388057 A CN105388057 A CN 105388057A CN 201510990333 A CN201510990333 A CN 201510990333A CN 105388057 A CN105388057 A CN 105388057A
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- CN
- China
- Prior art keywords
- dye liquor
- disposable
- dissolved
- dyeing
- ethyl alcohol
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001574 biopsy Methods 0.000 title claims abstract description 17
- 210000001156 gastric mucosa Anatomy 0.000 title abstract 3
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 claims abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical class OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000012153 distilled water Substances 0.000 claims abstract description 7
- SEACYXSIPDVVMV-UHFFFAOYSA-L eosin Y Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 SEACYXSIPDVVMV-UHFFFAOYSA-L 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 3
- 210000004877 mucosa Anatomy 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 5
- HLUCICHZHWJHLL-UHFFFAOYSA-N hematein Chemical compound C12=CC=C(O)C(O)=C2OCC2(O)C1=C1C=C(O)C(=O)C=C1C2 HLUCICHZHWJHLL-UHFFFAOYSA-N 0.000 claims description 5
- 239000000470 constituent Substances 0.000 claims description 2
- 238000004043 dyeing Methods 0.000 abstract description 17
- 239000000463 material Substances 0.000 abstract description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 abstract 4
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 abstract 1
- 230000001575 pathological effect Effects 0.000 description 10
- 239000012188 paraffin wax Substances 0.000 description 9
- 210000001519 tissue Anatomy 0.000 description 8
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000004575 stone Substances 0.000 description 5
- 239000001993 wax Substances 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 238000010827 pathological analysis Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses disposable dropping dye liquor for small samples in gastric mucosa biopsy and interventional operations, and belongs to the field of biotechnology. The disposable dropping dye liquor is characterized by being prepared from 2 g of eosin Y, 40 ml of absolute ethyl alcohol, 2 g of hematoxylin, 2 g of potassium dichromate, 400 ml of distilled water and 40 ml of saturated picric acid. A preparation method of the disposable dropping dye liquor includes the steps that eosin Y is dissolved in 20 ml of absolute ethyl alcohol, hematoxylin is dissolved in 20 ml of absolute ethyl alcohol, potassium dichromate is heated and dissolved in distilled water, all the materials are mixed after being dissolved, saturated picric acid is added, and the disposable dropping dye liquor is obtained after even mixing. The disposable dropping dye liquor can be applied to dyeing small samples in gastric mucosa biopsy and interventional operations and conventional sections and can also be applied to rapidly dyeing frozen sections, dyeing only takes 1 second, and a fresh purple red can be obtained after dyeing.
Description
Technical field
The present invention relates to a kind of dye liquor, particularly a kind of Mucosa Biopsy and intervene operation micro specimen disposable dye liquor, belong to biological technical field.
Background technology
Along with the development of science and technology, medical technology is advanced by leaps and bounds, and conversion direction, develop into present micro-wound no-damage technology by original large invasive techniques, constantly reduce the misery of patient.The development of gastroscope technology and Wicresoft's requirement, biopsy is drawn materials more and more less, and current biopsy specimen is in 1 millimeter; Get involved the puncture needle diameter of biopsy procedure below 0.5 millimeter, the diameter of organizing passed is about 0.1-0.5 millimeter.Tissue can be solved by the method for dripping dye lose or cross the problem of repairing sheet and repairing sheet deficiency.
A dye liquor for normal compound is that 2 grams, Yihong and 80% ethanol 400ml mix, and can only be used for conventional section statining, needs 3 minutes, and dyeing is in kermesinus.To organizing small sample dye-free effect.
Summary of the invention
The invention discloses a kind of Mucosa Biopsy and intervene operation micro specimen disposable dye liquor, this dye liquor both can be applied to Mucosa Biopsy and the dyeing of intervene operation small sample, also conventional section statining can be applied to, also can be used for quick frozen-section dyeing, needed for 1 second, dyeing is in light violet redness.
The concrete scheme that the present invention adopts is:
Mucosa Biopsy of the present invention and intervene operation micro specimen disposable dye liquor, comprise following effective constituent:
Wherein, add potassium dichromate and can increase mordant dyeing effect and colouring power; Adding saturated picric acid can make solution be faintly acid, improves the mordant dyeing effect of potassium dichromate; Make solution be faintly acid, increase the ability (cytoplasm is that alkalescent has acidophilia) that soda acid combines.Saturated picric acid is that weak acid is unlikely to disorganize structure.
The preparation method of Mucosa Biopsy of the present invention and intervene operation micro specimen disposable dye liquor is: Eosin Y be dissolved in 20ml absolute ethyl alcohol, haematine is dissolved in 20ml absolute ethyl alcohol, potassium dichromate heating for dissolving is in distilled water, mix after above-mentioned whole dissolving and add saturated picric acid, mix and namely obtain disposable dye liquor.
Mucosa Biopsy of the present invention and intervene operation micro specimen disposable dye liquor can be applicable to conventional section statining, also can be applied to Mucosa Biopsy and the dyeing of intervene operation small sample, also can be applicable to quick frozen-section dyeing.
Advantage of the present invention is:
1, disposable dye liquor dyeing time of the present invention is rapid, only needs just can complete dyeing 1 second.
2, this dye liquor Color is clear, and dyeing, in light violet redness, is convenient to visual inspection, is beneficial to embedding and makes colored wax stone, the pathological section that standard of making is complete.
3, scope more conventional dye liquor of this dye liquor application is extensive, both can be applied to Mucosa Biopsy and the dyeing of intervene operation small sample, and also can be applied to conventional section statining, and also can be applicable to quick frozen-section dyeing.
Accompanying drawing explanation
Fig. 1 is for the dye liquor described in the employing embodiment of the present invention 1 is to the wax stone picture of tissue staining.
Embodiment
Embodiment 1
2g Eosin Y be dissolved in 20ml absolute ethyl alcohol, 2g haematine is dissolved in 20ml absolute ethyl alcohol, and 2g potassium dichromate heating for dissolving, in 400ml distilled water, mixes after above-mentioned whole dissolving and adds 40ml and enter saturated picric acid, mix and namely obtain disposable dye liquor.
The process of pathological section diagnosis:
(1) in vitro tissue fixes 10% neutral buffered formalin 4-24 hour.
(2) pathologic sampling, adopts the above-mentioned disposable dye liquor prepared to dye to Pathologic specimen.
(3) tissue dewatering, transparent, waxdip about 12 hours.
(4) paraffin embedding prepares colored wax stone.
(5) paraffin section.
(6) Yihong liquid in the above-mentioned replaceable conventional dye liquor of disposable dye liquor prepared is adopted to dye to paraffin section.
(7) mounting.
(8) basis of microscopic observation, makes pathological diagnosis.
Embodiment 2
1.7g Eosin Y is dissolved in 20ml absolute ethyl alcohol, 1.7g haematine is dissolved in 20ml absolute ethyl alcohol, 1.6g potassium dichromate heating for dissolving, in 400ml distilled water, mixes after above-mentioned whole dissolving and adds the saturated picric acid of 30ml, mix and namely obtain disposable dye liquor.
The process of pathological section diagnosis:
(1) in vitro tissue fixes 10% neutral buffered formalin 4-24 hour.
(2) pathologic sampling, adopts the above-mentioned disposable dye liquor prepared to dye to Pathologic specimen.
(3) tissue dewatering, transparent, waxdip about 12 hours.
(4) paraffin embedding prepares colored wax stone.
(5) paraffin section.
(6) Yihong liquid in the above-mentioned replaceable conventional dye liquor of disposable dye liquor prepared is adopted to dye to paraffin section.
(7) mounting.
(8) basis of microscopic observation, makes pathological diagnosis.
Embodiment 3
2.3g Eosin Y is dissolved in 20ml absolute ethyl alcohol, 2.3g haematine is dissolved in 20ml absolute ethyl alcohol, 2.2g potassium dichromate heating for dissolving, in 400ml distilled water, mixes after above-mentioned whole dissolving and adds the saturated picric acid of 50ml, mix and namely obtain disposable dye liquor.
The process of pathological section diagnosis:
(1) in vitro tissue fixes 10% neutral buffered formalin 4-24 hour.
(2) pathologic sampling, adopts the above-mentioned disposable dye liquor prepared to dye to Pathologic specimen.
(3) tissue dewatering, transparent, waxdip about 12 hours.
(4) paraffin embedding prepares colored wax stone.
(5) paraffin section.
(6) Yihong liquid in the above-mentioned replaceable conventional dye liquor of disposable dye liquor prepared is adopted to dye to paraffin section.
(7) mounting.
(8) basis of microscopic observation, makes pathological diagnosis.
Embodiment 1 is preferred embodiments of the present invention.
Claims (2)
1. Mucosa Biopsy and intervene operation micro specimen disposable dye liquor, is characterized in that, comprise following effective constituent:
2. Mucosa Biopsy according to claim 1 and intervene operation micro specimen disposable dye liquor, it is characterized in that, the preparation method of disposable described dye liquor is:
Eosin Y be dissolved in 20ml absolute ethyl alcohol, haematine is dissolved in 20ml absolute ethyl alcohol, and potassium dichromate heating for dissolving, in distilled water, mixes after above-mentioned whole dissolving and adds saturated picric acid, mix and namely obtain disposable dye liquor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510990333.8A CN105388057A (en) | 2015-12-25 | 2015-12-25 | Disposable dropping dye liquor for small samples in gastric mucosa biopsy and interventional operations |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510990333.8A CN105388057A (en) | 2015-12-25 | 2015-12-25 | Disposable dropping dye liquor for small samples in gastric mucosa biopsy and interventional operations |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105388057A true CN105388057A (en) | 2016-03-09 |
Family
ID=55420598
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510990333.8A Pending CN105388057A (en) | 2015-12-25 | 2015-12-25 | Disposable dropping dye liquor for small samples in gastric mucosa biopsy and interventional operations |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105388057A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8945941B2 (en) * | 2006-05-26 | 2015-02-03 | Brandeis University | Tissue sample preparation and MALDI MS imaging thereof |
| CN104893359A (en) * | 2015-06-02 | 2015-09-09 | 运城市妇幼保健院 | Rapid cast-off cell sample dyeing agent and preparation method thereof |
| CN104897453A (en) * | 2015-05-27 | 2015-09-09 | 四川农业大学 | Rapid organ tissue dyeing method |
-
2015
- 2015-12-25 CN CN201510990333.8A patent/CN105388057A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8945941B2 (en) * | 2006-05-26 | 2015-02-03 | Brandeis University | Tissue sample preparation and MALDI MS imaging thereof |
| CN104897453A (en) * | 2015-05-27 | 2015-09-09 | 四川农业大学 | Rapid organ tissue dyeing method |
| CN104893359A (en) * | 2015-06-02 | 2015-09-09 | 运城市妇幼保健院 | Rapid cast-off cell sample dyeing agent and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 李喜强等: "《基础医学实验与指导 上册》", 31 December 2007 * |
| 涂琦等: "《苏木素伊红染色一步法的研究及应用》", 《江西医药》 * |
| 王永军等: "《H E 中胞浆染色液的改进及染色体会》", 《THE 5TH CHINESE CONFERENCE ON ONCOLOGY》 * |
| 王永军等: "《伊红染液的改良及使用体会》", 《诊断病理学杂志》 * |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160309 |