CN104893359A - Rapid cast-off cell sample dyeing agent and preparation method thereof - Google Patents
Rapid cast-off cell sample dyeing agent and preparation method thereof Download PDFInfo
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- CN104893359A CN104893359A CN201510309980.8A CN201510309980A CN104893359A CN 104893359 A CN104893359 A CN 104893359A CN 201510309980 A CN201510309980 A CN 201510309980A CN 104893359 A CN104893359 A CN 104893359A
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Abstract
The invention relates to a rapid cast-off cell sample dyeing agent and a preparation method thereof. The rapid cast-off cell sample dyeing agent comprises the following components in parts by weight: 3.5 parts of haematoxylin, 45 parts of absolute ethyl alcohol, 50 parts of aluminum potassium sulfate dodecahydrate, 500 parts of distilled water, 2 parts of mercuric oxide and 6 parts of glacial acetic acid. The preparation method of the rapid cast-off cell sample dyeing agent comprises the following steps: firstly putting aluminum potassium sulfate dodecahydrate into distilled water, heating to about 80 DEG C for dissolving aluminum potassium sulfate dodecahydrate, and cooling to normal temperature, so that an aluminum potassium sulfate dodecahydrate dissolved solution is obtained; and adding haematoxylin into absolute ethyl alcohol, dissolving, mixing with the aluminum potassium sulfate dodecahydrate dissolved solution, and then sequentially adding mercuric oxide and glacial acetic acid, so that the rapid cast-off cell sample dyeing agent is obtained. A cast-off cell smear sample is obtained by dyeing for 30+/-s by utilizing the rapid cast-off cell sample dyeing agent and washing for 10+/-s with flowing water. Compared with the prior art, the rapid cast-off cell sample dyeing agent has the beneficial effects that the dyeing time is shortened to 1 minute from 1 hour, the clinical diagnosis time is much earlier, a method is simple and easy to learn, and the cost of used raw materials is low; meanwhile, the time consumed by inspection personnel is less, the labour cost is low, and the financial burden of a patient is low.
Description
Technical field
The present invention relates to microscopy specimen staining agent and preparation method thereof in a kind of medical field cell pathology Morphologic Diagnosis, particularly relate to a kind of cast-off cells sample rapid dyeing agent and preparation method thereof.
Background technology
At present, cast-off cells pathological diagnosis, hematoxylin-eosin staining procedures (HE staining) that cell smear routine uses or Papanicolaou's vaginal smear technique; As HE staining step: 1, smear fixes 10
±min, 2, washing 2-3min, 3, hematoxylin liquid 2min, 4, wash 2-3 time from the beginning, 5,0.5% aqueous hydrochloric acid 2-3s, 6, tap water 2-3 time, 7, Quilonum Retard liquid 1min, 8, each 1min of 95% ethanol I, II, 9, each 30s of Yihong liquid 1min, 10,95% ethanol I, II, III, IV, 11, each 30s of dehydrated alcohol I, II, 12, each 30s of dimethylbenzene I, II, 13, neutral gum sealing.As Papanicolaou's vaginal smear technique step: 1, smear fixes 10
±min, 2, washing 3-5min, 3, hematoxylin liquid 2min, 4, clean 2-3 time in water, 5, (100ml adds unsaturated carbonate lithium 1) 1-2min is broken up, 6, clean 2-3 time in water, 7,95% ethanol 30s, 8, each 1 time of orange G liquid 0.5-1min, 9,95% ethanol I, II, 10, EA
50the each 30s of 2min, 11,95% ethanol I, II, III, IV, 12, each 30s of dehydrated alcohol I, II, 13, each 30s of dimethylbenzene I, II, 14, neutral gum sealing.Prior art is because of dyeing procedure complexity, step many (13 ~ 14 step), time-consuming length (about 1 hour), can not microscopy immediately, clinically provide the diagnosis report time long, patient will wait for a long time, not only affect diagnosis and treatment process, incur loss through delay patient's inspection and examine the time, and reviewer is consuming time many, cost of labor is high, and the economical load of patient is large.
Summary of the invention
Object of the present invention is exactly to overcome the deficiencies in the prior art, provides a kind of cast-off cells sample rapid dyeing agent and preparation method thereof.
The technical scheme adopted for realizing the object of the invention is:
The agent of a kind of cast-off cells sample rapid dyeing, is characterized in that: comprise following component by weight percentage: hematoxylin 3.5 parts, dehydrated alcohol 45 parts, the bright vanadium of Potassium 50 parts, distilled water 500 parts, 2 parts, red precipitate, 6 parts, Glacial acetic acid.
A preparation method for cast-off cells sample rapid dyeing agent, is characterized in that: comprise following component by weight percentage: hematoxylin 3.5 parts, dehydrated alcohol 45 parts, the bright vanadium of Potassium 50 parts, distilled water 500 parts, 2 parts, red precipitate, 6 parts, Glacial acetic acid; First bright for Potassium vanadium is put into distilled water to be heated to about 80 degree and to make it dissolve, cool to normal temperature, the obtained bright vanadium lysate of Potassium; After hematoxylin being added anhydrous alcohol solution, vanadium lysate bright with Potassium mixes, and then adds red precipitate, Glacial acetic acid successively, obtained cast-off cells sample rapid dyeing agent.
Using method of the present invention: 1, cast-off cells smear preparation cast-off cells sample of the present invention rapid dyeing agent dyeing 30
±s, 2, flowing water washes 10
±s completes.
The present invention's beneficial effect is compared with prior art: shorten dyeing time, and shortened in 1 minute by 1 hour and complete, the clinical diagnosis time greatly shifts to an earlier date, the easy easy grasp of method, and cost of raw materials used is low; Reviewer is consuming time few, and cost of labor is low, and the economical load of patient is little.
Accompanying drawing explanation
Fig. 1 be adopt normal dyeing method to obtain microscopy photo;
Fig. 2 adopts cast-off cells sample rapid dyeing agent of the present invention to dye the microscopy photo obtained.
Icon illustrates: 1. nucleus; 2. nucleus.
Embodiment
The present invention is further illustrated below by embodiment.
The agent of a kind of cast-off cells sample rapid dyeing, comprises following component: hematoxylin 3.5 grams, dehydrated alcohol 45 grams, the bright vanadium of Potassium 50 grams, distilled water 500 grams, 2 grams, red precipitate, 6 grams, Glacial acetic acid by weight percentage.
The preparation method of above-mentioned cast-off cells sample rapid dyeing agent is: first bright for Potassium vanadium is put into distilled water and be heated to about 80 degree and make it dissolve, cool to normal temperature, the obtained bright vanadium lysate of Potassium; After hematoxylin being added anhydrous alcohol solution, vanadium lysate bright with Potassium mixes, and then adds red precipitate, Glacial acetic acid successively, obtained cast-off cells sample rapid dyeing agent.
Using method of the present invention: 1, cast-off cells smear preparation cast-off cells sample of the present invention rapid dyeing agent dyeing 30
±s, 2, flowing water washes 10
±s completes.
Experimental result:
Accompanying drawing 1 be adopt normal dyeing method to obtain microscopy photo; Accompanying drawing 2 adopts cast-off cells sample rapid dyeing agent of the present invention to dye the microscopy photo obtained; As can be seen from the figure, the result that this staining and normal dyeing method obtain is basically identical, and cellular form and structural integrity, edge clear, meets coloration requirements completely.Cast-off cells sample rapid dyeing agent of the present invention dyeing is adopted to obtain coloration result humidity strip identical with dry plate effect.
Meet microscopy requirement completely through clinical application the present invention, the easy easy grasp of method, does not need special conditions, as long as possess Cytological Basis knowledge, the cytologic specimen of collection, after present method rapid dyeing, just can carry out cell pathology Morphologic Diagnosis; Receive slide sample in 1 minute and complete dyeing course; Smear preparation with to dye, can get final product microscopy, avoids patient wait marquis for a long time or come and go the disadvantage of getting diagnostic result more a long way, saves the time that patient is a large amount of, be very easy to patient; The five poisonous creatures: scorpion, viper, centipede, house lizard, toad of the present invention, environmental protection, can decreasing pollution, reduce the injury to operator's health; With low cost, can patient economy burden be alleviated.
The present invention is not limited to the preparation method described in embodiment; its description is illustrative; and it is nonrestrictive; authority of the present invention limited by claim; can change according to the present invention based on the art personnel, technology related to the present invention that the method such as restructuring obtains and product, all within protection scope of the present invention.
Claims (2)
1. a cast-off cells sample rapid dyeing agent, is characterized in that: comprise following component by weight percentage: hematoxylin 3.5 parts, dehydrated alcohol 45 parts, the bright vanadium of Potassium 50 parts, distilled water 500 parts, 2 parts, red precipitate, 6 parts, Glacial acetic acid.
2. a preparation method for cast-off cells sample rapid dyeing agent, is characterized in that: comprise following component by weight percentage: hematoxylin 3.5 parts, dehydrated alcohol 45 parts, the bright vanadium of Potassium 50 parts, distilled water 500 parts, 2 parts, red precipitate, 6 parts, Glacial acetic acid; First bright for Potassium vanadium is put into distilled water to be heated to about 80 degree and to make it dissolve, cool to normal temperature, the obtained bright vanadium lysate of Potassium; After hematoxylin being added anhydrous alcohol solution, vanadium lysate bright with Potassium mixes, and then adds red precipitate, Glacial acetic acid successively, obtained cast-off cells sample rapid dyeing agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510309980.8A CN104893359A (en) | 2015-06-02 | 2015-06-02 | Rapid cast-off cell sample dyeing agent and preparation method thereof |
Applications Claiming Priority (1)
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| CN201510309980.8A CN104893359A (en) | 2015-06-02 | 2015-06-02 | Rapid cast-off cell sample dyeing agent and preparation method thereof |
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| CN104893359A true CN104893359A (en) | 2015-09-09 |
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| CN201510309980.8A Pending CN104893359A (en) | 2015-06-02 | 2015-06-02 | Rapid cast-off cell sample dyeing agent and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105388057A (en) * | 2015-12-25 | 2016-03-09 | 贾振军 | Disposable dropping dye liquor for small samples in gastric mucosa biopsy and interventional operations |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3440317A (en) * | 1964-11-09 | 1969-04-22 | Arfilio J Martinez | Cell coloring process and composition for cytological examination |
| US20120276584A1 (en) * | 2007-03-15 | 2012-11-01 | Kosmeder Jerome W | Stabilized hematoxylin |
| CN103694732A (en) * | 2013-12-31 | 2014-04-02 | 江苏省原子医学研究所 | Hematoxylin staining solution and HE (hematoxylin eosin) staining solution containing hematoxylin staining solution |
| CN103725040A (en) * | 2013-12-31 | 2014-04-16 | 江苏省原子医学研究所 | Hematoxylin eosin staining solution and preparation method thereof |
-
2015
- 2015-06-02 CN CN201510309980.8A patent/CN104893359A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3440317A (en) * | 1964-11-09 | 1969-04-22 | Arfilio J Martinez | Cell coloring process and composition for cytological examination |
| US20120276584A1 (en) * | 2007-03-15 | 2012-11-01 | Kosmeder Jerome W | Stabilized hematoxylin |
| CN103694732A (en) * | 2013-12-31 | 2014-04-02 | 江苏省原子医学研究所 | Hematoxylin staining solution and HE (hematoxylin eosin) staining solution containing hematoxylin staining solution |
| CN103725040A (en) * | 2013-12-31 | 2014-04-16 | 江苏省原子医学研究所 | Hematoxylin eosin staining solution and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 游跃星等: "阴道脱落细胞苏木紫-伊红染色法步骤的改进", 《现代检验医学杂志》 * |
| 陈彦杰等: "Harris 苏木素染液配制过氧化和使用防氧化控制的探讨", 《中国当代医药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105388057A (en) * | 2015-12-25 | 2016-03-09 | 贾振军 | Disposable dropping dye liquor for small samples in gastric mucosa biopsy and interventional operations |
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Application publication date: 20150909 |
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