CN105385587B - A kind of noncontacting proximity sensor joint and its application - Google Patents
A kind of noncontacting proximity sensor joint and its application Download PDFInfo
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- CN105385587B CN105385587B CN201510981653.7A CN201510981653A CN105385587B CN 105385587 B CN105385587 B CN 105385587B CN 201510981653 A CN201510981653 A CN 201510981653A CN 105385587 B CN105385587 B CN 105385587B
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- Prior art keywords
- proximity sensor
- noncontacting proximity
- basal disc
- transparent cut
- sensor probe
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
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- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract
The present invention relates to a kind of noncontacting proximity sensor joint and its application.The noncontacting proximity sensor joint of the present invention includes the sensor probe chamber resettling of basal disc and opening, the basal disc is provided with transparent cut-off, the sensor probe chamber resettling is connected with the basal disc and is provided with conversion film positioned at the side of the transparent cut-off, the opposite side of transparent cut-off.The noncontacting proximity sensor joint of the present invention is particularly suitable for use in enclosed cell culture apparatus.
Description
Technical field
The present invention relates to noncontacting proximity sensor joint and its application.
Background technology
Traditional cell culture is cultivated with culture dish or blake bottle in carbon dioxide incubator, will to condition of culture
Ask comparison high, it is necessary to be operated under super-clean bench, then be placed into CO2gas incubator and cultivate, and take out to be placed into good time and lead
Cause micro- Microscopic observation cell growth state.
For the pH value in nutrient solution in cell cultivation process, the measurement of dissolved oxygen amount, contact measurement is generally required, easily
Cause pollution.Even if strict sterile working, it is also difficult to avoid the not contaminated danger of cell, be especially used for clinic in culture
During the human immunocyte of research or clinical treatment, once cell is contaminated, the not only culture to cell has an impact, while may
Threaten the life of patient.
The content of the invention
For in the prior art the defects of, the present invention various risk factors present in the cell cultivation process consider, knot
Close the various conditions of cell culture, there is provided a kind of noncontacting proximity sensor joint and application, can need not contact sample, in real time prison
Control the index of correlation of cell culture fluid.
Present invention firstly provides a kind of noncontacting proximity sensor joint, including basal disc and the sensor probe of opening to house
Chamber, the basal disc are provided with transparent cut-off, and the sensor probe chamber resettling is connected with the basal disc and positioned at described
The side of transparent cut-off, the opposite side of transparent cut-off are provided with conversion film.
Preferably, the transparent cut-off water white transparency, both sides are smooth.
The sensor probe chamber resettling can be with sensor probe grafting or threaded connection.
Preferably, the sensor probe chamber resettling is provided with internal thread.
The film of changing causes color-changing membrane or dissolved oxygen to cause color-changing membrane into pH.
It is further preferred that the outer for the side that the transparent cut-off is provided with conversion film is provided with the bead for protruding from basal disc 1.
It also can be detachable connection that the sensor probe chamber resettling can be fixedly connected with basal disc.Detachable connection
Mode includes but is not limited to grafting, threaded connection etc..
Preferably, the sensor probe chamber resettling and basal disc junction rounding off.
The noncontacting proximity sensor joint of the present invention can be used for enclosed cell culture apparatus.
The cell culture apparatus includes but is not limited to cell culture bags.
The design of the noncontacting proximity sensor joint of the present invention, the matched sensor used can be caused without sterilizing
Sterilization, not only operate it is more convenient, and can more preferable Pollution protection.
Brief description of the drawings
Fig. 1 is shown as the noncontacting proximity sensor joint schematic diagram of the embodiment of the present invention.
Fig. 2 is shown as the sectional view of the noncontacting proximity sensor joint of the embodiment of the present invention.
Fig. 3 is shown as the stereogram of the noncontacting proximity sensor joint of the embodiment of the present invention.
Component label instructions
1 basal disc
11 transparent cut-offs
2 sensor probe chamber resettling
21 internal threads
3 beads
Embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book understands other advantages and effect of the present invention easily.
Fig. 1 is referred to Fig. 3.It should be clear that structure, ratio, size depicted in this specification institute accompanying drawings etc., only to
Coordinate the content disclosed in specification, so that those skilled in the art understands and reads, being not limited to the present invention can be real
The qualifications applied, therefore do not have technical essential meaning, the tune of the modification of any structure, the change of proportionate relationship or size
It is whole, in the case where not influenceing the effect of present invention can be generated and the purpose that can reach, all should still fall in disclosed skill
Art content is obtained in the range of covering.Meanwhile in this specification it is cited as " on ", " under ", "left", "right", " centre " and
The term of " one " etc., understanding for narration is merely convenient to, and is not used to limit the enforceable scope of the present invention, its relativeness
It is altered or modified, in the case where changing technology contents without essence, when being also considered as the enforceable category of the present invention.
Noncontacting proximity sensor joint as Figure 1-3, including basal disc 1 and open sensor probe chamber resettling 2,
The basal disc 1 is provided with transparent cut-off 11, and the sensor probe chamber resettling 2 is connected with the basal disc 1 and positioned at described
The side of transparent cut-off 11, the opposite side of transparent cut-off 11 are provided with conversion film.
The sensor probe chamber resettling of the opening refers to that the chamber is provided with opening, and the opening can be used for convenient pass
Sensor probe insertion.
The conversion film is respectively arranged on the both sides of the transparent cut-off 11 with the sensor probe chamber resettling 2.Fig. 2 institutes
Show that arrow points to the side for being provided with conversion film.In use, the conversion film is in contact with testing liquid, and sensor probe is fixed
It is relative with conversion film and isolate with testing liquid through transparent in sensor probe chamber resettling.
The basal disc can install container with prepare liquid and engage., can be by the side of the basal disc 1 by taking cell culture bags as an example
Face or edge and the cell culture bags phase welding or welding, cell culture bags correspond to it is transparent cut-off 11 position be provided with open
Mouthful, the opening causes the conversion film on transparent cut-off to be in contact with nutrient solution in cell culture bags, and inserts sensor probe
The sensor probe of chamber resettling 2 is then isolated due to the presence of transparent cut-off 11 with nutrient solution in cell culture bags.
Preferably, 11 water white transparencies of the transparent cut-off, both sides are smooth.Smooth transparent cut-off is more conducive to change film color
Through.
The transparent cut-off 11 is not limited to only be located at the middle part of the basal disc 1, only needs transparent cut-off periphery to leave necessarily
The basal disc of width is enough to be combined with cell culture bags.
The sensor probe chamber resettling 2 can be with sensor probe grafting or threaded connection.
In a preferred embodiment, the sensor probe chamber resettling 2 is provided with internal thread 21.Matching, the sensing
Device probe is provided with external screw thread.Threaded connection may further ensure that the stability of sensor probe detection.
The film of changing causes color-changing membrane or dissolved oxygen to cause color-changing membrane into pH.
PH, which causes color-changing membrane to refer to, to convert printing opacity color according to the pH value of contact liq.PH cause color-changing membrane can by pH indicator with
It is film-made and obtains after filmogen mixture.PH causes pH described in color-changing membrane to cause color-changing membrane to belong to prior art, as used in pH electrodes
PH sensing membranes etc..
Change film causes the sensor connector of color-changing membrane to be engaged with RGB sensors and white light source into pH.It detects pH
The mechanism of value is：PH causes color-changing membrane to penetrate the film according to the pH value turn colors of its contact liq so as to influence white light source
Color, white light source coordinate pH to cause color-changing membrane that pH signals can be changed into color signal, the detection of RGB sensors penetrate conversion film and
The color signal of transparent cut-off, the pH of prepare liquid in prepare liquid installation container is known according to the relation of conversion film color and pH value
Value, changing the relation of film color and pH value can be obtained using the standard liquid detection of known pH value in advance.
Dissolved oxygen causes color-changing membrane to convert printing opacity color according to the dissolved oxygen value of contact liq.Dissolved oxygen causes color-changing membrane can be by
Oxygen indicator is dissolved to obtain with being film-made after filmogen mixture.Dissolved oxygen causes dissolved oxygen described in color-changing membrane to cause color-changing membrane to belong to existing
Technology.
Change film causes the sensor connector of color-changing membrane to be engaged with RGB color sensor and white light source into dissolved oxygen.
It detects the mechanism of oxygen dissolving value：Dissolved oxygen causes dissolved oxygen concentration turn colors of the color-changing membrane according to its contact liq, so as to shadow
The color that white light source penetrates the film is rung, white light source coordinates dissolved oxygen to cause color-changing membrane that dissolving oxygen signal can be changed into color letter
Number, the detection of RGB color sensor penetrates the color signal of conversion film and transparent cut-off, according to color and the relation of dissolved oxygen concentration
Know the dissolved oxygen value of prepare liquid in prepare liquid installation container, the relation changed between film color and dissolved oxygen can utilize in advance
The standard liquid detection of known dissolved oxygen concentration obtains.
In order to preferably protect conversion film, the outer for the side that the transparent cut-off 11 is provided with conversion film, which is provided with, protrudes from base
The bead 3 of disk 1.The bead can alleviate nutrient solution for changing the impact of film to further ensure that the steady of sensor probe detection
It is qualitative.
It also can be detachable connection that the sensor probe chamber resettling 2 can be fixedly connected with basal disc 1.Detachable company
The mode of connecing includes but is not limited to grafting, threaded connection etc..
Preferably, the sensor probe chamber resettling 2 and the junction rounding off of basal disc 1.
The noncontacting proximity sensor joint of the present invention is particularly suitable for use in cell culture bags.
By the noncontacting proximity sensor joint of the present invention, the pH of prepare liquid and dissolved oxygen situation can in prepare liquid installation container
Color signal is converted into, by accessing RGB sensor probes in sensor probe chamber resettling, you can turn color signal
Level signal or square-wave signal are changed to, the controller of RGB sensors reads level signal or square-wave signal, according to conversion film color
Corresponding level signal and pH value and the transformational relation of dissolved oxygen, obtain the pH value and oxygen dissolving value of cell culture fluid.Change film color
The transformational relation of corresponding level signal and pH value and dissolved oxygen can be by routine techniques, such as beforehand through to known pH value or dissolved oxygen
Curve is drawn after the standard liquid detection of value to obtain.
In summary, the present invention effectively overcomes various shortcoming of the prior art and has high industrial utilization.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (9)
- A kind of 1. noncontacting proximity sensor joint suitable for cell culture bags, it is characterised in that the biography including basal disc and opening Sensor probe chamber resettling, the basal disc are provided with transparent cut-off, and the sensor probe chamber resettling is connected with the basal disc Connect and be provided with conversion film positioned at the side of the transparent cut-off, the opposite side of transparent cut-off, in use, the conversion film is with treating Liquid is surveyed to be in contact, and sensor probe is fixed in sensor probe chamber resettling, and and testing liquid relative with conversion film Isolate through transparent cut-off.
- 2. noncontacting proximity sensor joint as claimed in claim 1, it is characterised in that the transparent cut-off water white transparency, two Smooth-sided compression candles.
- 3. noncontacting proximity sensor joint as claimed in claim 1, it is characterised in that the transparent cut-off is located at the basal disc Middle part.
- 4. noncontacting proximity sensor joint as claimed in claim 1, it is characterised in that the sensor probe chamber resettling sets There is internal thread.
- 5. noncontacting proximity sensor joint as claimed in claim 1, it is characterised in that it is described change film into pH cause color-changing membrane or Dissolved oxygen causes color-changing membrane.
- 6. noncontacting proximity sensor joint as claimed in claim 1, it is characterised in that the transparent cut-off is provided with conversion film The outer of side is provided with the bead for protruding from basal disc.
- 7. noncontacting proximity sensor joint as claimed in claim 1, it is characterised in that the sensor probe chamber resettling with Basal disc junction rounding off.
- 8. the noncontacting proximity sensor joint as described in claim 1-7 any claims is used for closed cell culture apparatus Purposes.
- 9. purposes as claimed in claim 8, the cell culture apparatus is cell culture bags.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510981653.7A CN105385587B (en) | 2015-12-23 | 2015-12-23 | A kind of noncontacting proximity sensor joint and its application |
| US16/064,414 US10934513B2 (en) | 2015-12-23 | 2016-12-15 | Fully automated continuous cell culture system |
| PCT/CN2016/110035 WO2017107837A1 (en) | 2015-12-23 | 2016-12-15 | Fully automated continuous cell culture system |
| JP2018533724A JP6889723B2 (en) | 2015-12-23 | 2016-12-15 | Cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510981653.7A CN105385587B (en) | 2015-12-23 | 2015-12-23 | A kind of noncontacting proximity sensor joint and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105385587A CN105385587A (en) | 2016-03-09 |
| CN105385587B true CN105385587B (en) | 2017-12-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510981653.7A Active CN105385587B (en) | 2015-12-23 | 2015-12-23 | A kind of noncontacting proximity sensor joint and its application |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5164796A (en) * | 1988-03-15 | 1992-11-17 | Akzo N.V. | Apparatus and method for detection of microorganisms |
| CN101082578A (en) * | 2006-05-30 | 2007-12-05 | 上海理工大学 | System for detecting light transmission substance thermophysical variation with electrical signal |
| CN201289406Y (en) * | 2008-11-14 | 2009-08-12 | 常州市惠昌传感器有限公司 | Temperature sensor joint |
| DE102009037345A1 (en) * | 2009-06-16 | 2010-12-23 | Sartorius Stedim Biotech Gmbh | Container with a sensor adapter |
| CN103162851A (en) * | 2011-12-15 | 2013-06-19 | 西安民生电热技术工程有限责任公司 | Thermosensitive temperature sensor connector with socket |
| FR2992722B1 (en) * | 2012-06-28 | 2015-05-29 | Commissariat Energie Atomique | LOCALIZATION OF LEAKAGE IN A CANALIZATION |
| CN203999619U (en) * | 2014-07-17 | 2014-12-10 | 上海美凯纯生物科技有限公司 | A kind of microorganism fast culture proofing unit |
| CN205420410U (en) * | 2015-12-23 | 2016-08-03 | 上海吉凯基因科技有限公司 | Noncontacting proximity sensor connects |
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| CN105385587A (en) | 2016-03-09 |
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| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20160309 Assignee: China (Shanghai) Biological Medicine Co Ltd Assignor: SHANGHAI JIKAI GENE TECHNOLOGY CO., LTD. Contract record no.: 2017310000066 Denomination of invention: Non-contact sensor connector and application thereof Granted publication date: 20171205 License type: Exclusive License Record date: 20171207 |
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| EE01 | Entry into force of recordation of patent licensing contract | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171227 Address after: Room 101-7, No. 332, Edison Road, China (Shanghai) free trade test area, Shanghai Patentee after: Shanghai Jibi Biotechnology Co., Ltd. Address before: 201203 room 101-A, No. 328 Edison Road, Zhangjiang hi tech park, Shanghai, Pudong New Area Patentee before: SHANGHAI JIKAI GENE TECHNOLOGY CO., LTD. |
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| TR01 | Transfer of patent right |