CN105381503B - A kind of artificial skin of high activity - Google Patents

A kind of artificial skin of high activity Download PDF

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Publication number
CN105381503B
CN105381503B CN201510915412.2A CN201510915412A CN105381503B CN 105381503 B CN105381503 B CN 105381503B CN 201510915412 A CN201510915412 A CN 201510915412A CN 105381503 B CN105381503 B CN 105381503B
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weight
parts
skin
artificial skin
nano chitosan
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CN105381503A (en
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顾银凤
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Qingdao West Kai Biological Technology Co., Ltd.
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Qingdao West Kai Biological Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/416—Anti-neoplastic or anti-proliferative or anti-restenosis or anti-angiogenic agents, e.g. paclitaxel, sirolimus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Materials For Medical Uses (AREA)
  • Cosmetics (AREA)

Abstract

The present invention discloses a kind of artificial skin and manufacturing method；The artificial skin has preferable surface tension, plentiful elastic form, has good inhibition bacteria characteristic, is organized as the polymer of Nano chitosan and polypeptide.The artificial skin benefits be this skin biomedicine characteristic is good, medical value is high, technological process is short, operation is easy, save equipment reduce cost.With preferable application prospect and application value.

Description

A kind of artificial skin of high activity
Technical field
The present invention relates to medical ancillary technique field, especially a kind of skin-grafting artificial skin.
Background technology
Artificial skin has extensive medical application foreground in terms of the treatment of burn, scald etc., therefore receives both at home and abroad The extensive concern and in-depth study of scholar.The skin of burn patient faces tissue fluid exudation, infection (especially in recovery process It is charrin's disease) a series of problems, such as.Currently, clinically using high permeability steam film as dressing, including the U.S. more The Tegaderm of 3M companies, Britain OpSite etc. add propylene type adhesive in these dressing comprising polyurethane, these adhesives jail Disjunctor hair is adhered, pain is be easy to cause when removing, it is uncomfortable even to damage；And these dressing can not effectively solve tissue fluid The problems such as exudation, infection.
In clinical treatment, to accelerate wound healing, promote body cell growth, skin injury need to keep certain wetting State, that is, require wound can neither overdrying, and be unable to excessive moisture.And made of several process programs of the prior art Artificial skin over the course for the treatment of, due to the limitation of itself structure, tend not to skin injury build a suitable skin, The environment of Organism Growth, to affect the treatment.Therefore, present clinical unit is in treatment burn practice, still to artificial skin With larger demand.
Invention content
Present invention aim to overcome above-mentioned the shortcomings of the prior art, providing one kind having some strength and bullet Property, permeability is good, can closely be attached with the surface of a wound, and can observe the surface of a wound, sterile, nontoxic, and no antigen or antigenicity are small, to wound Face is non-stimulated or irritation is smaller, and has stronger bacteria resistance function；It is safe and convenient to use, the surface of a wound can be protected not infected, do not hindered Self skin and skin-grafting plate is hindered to grow, wound healing, scar is smaller, and raw material sources are extensive, can manufacture, and cost is relatively low, Easy long-term preservation, and just with the manufacturing method of the artificial skin of transport and the artificial skin.
It is an object of the invention to：For treatment skin trauma, there is an urgent need to provide a kind of inhibition bacterium life for patient It is long, and at the same time with preferable active artificial skin.
It is a further object to provide the processes for producing this artificial skin.
A kind of manufacturing method of artificial skin, is measured in parts by weight, using following proportioning raw materials：Nano chitosan 120, Polypeptide 5, lactic acid 5, organic acid 75, surfactant 30, absolute viscosity are the polyacrylic acid 60 of 4764mpa.s, deionized water 950；And is manufactured in the steps below：The first step, the preparation of A material：Remove 950 parts by weight of ionized water, 75 parts by weight of organic acid, Absolute viscosity is 60 parts by weight of polyacrylic acid of 4764mpa.s, is placed in reaction kettle, in 50 DEG C, 3000 turns/min stirring actions Lower dissolving 1.5 hours becomes the A material that pH value is 3.5-4.0, parts by weight are 1085；Second step, the preparation of B material：After A material are made, The temperature of A material in reaction kettle is kept to be stirred to it at 50 DEG C and at 3000 turns/min, with the time of 30min gradually by 120 weights The Nano chitosan of amount part is added in A material, until Nano chitosan is dissolved almost completely, becomes Nano chitosan solution, referred to as B Material；Third walks, the preparation of Nano chitosan and amino acid or the polymer capsules of lactic acid：After B material are made, by the B in reaction kettle Its temperature is gradually heated up by material under 3000 turns/min stirrings and 40-100W ultrasonic wave collective effects in 1.5 hours from 45 DEG C To 65 DEG C, then, the surfactant of 30 parts by weight is added, continued under aforementioned stirring and ultrasonic wave collective effect, gradually The lactic acid of 5 parts by weight and the polypeptide of 5 parts by weight is added（SEQ ID NO：Shown in 1-15）, do not stopping stirring and ultrasonic wave work Polymerisation is carried out under 2 hours, the temperature in reaction kettle is then gradually increased to 90 DEG C, and herein from 65 DEG C with 1.5 hours At a temperature of keep polymerisation 1 hour, polymerisation to accuse and finish, filter while hot, that is, obtain light salmon Nano chitosan and ammonia The polymer capsules of base acid or lactic acid；The polymer capsules of Nano chitosan and amino acid or lactic acid are processed into and receive by the 4th step Rice chitosan artificial skin：By the polymer capsules of Nano chitosan and amino acid or lactic acid, it is washed till with 40 DEG C of deionized water Neutrality, and shear film forming, then by this film at a temperature of 50 DEG C dry 1.5 hours to get to artificial skin.
This nanometer artificial skin is impregnated with translucent membrane body, in physiological saline with soft and full elasticity Form has the good characteristic for inhibiting bacterium and antitumor cell to grow in biomedicine while having preferable skin Smoothness and moisture.
The nanometer artificial skin that will have been dried, strictly carries out disinfection as required, then seals, packs, storage preserves.
Surfactant used can be Tween-20, peregal 0S-10, the efficient Uniform stains of peregal 0S-15, JFZ-1, JFZ bleeding agents or other fats polyvingl ethers；
Nano chitosan used, for the Nano chitosan product with following physical and chemical index：Powder granule>80 mesh, powder Body volume density is 0. 56-0. 62g/ml, powder moisture San 5. 6%, and the granularity of Nano chitosan particle is 20-60nm, again Metals content impurity：Lead<0. lmg/kg, mercury<0. lmg/kg, arsenic<0. 2mg/kg dissolves in weak acid, diluted acid or 6 third Ketone, it is sterile；
Ultrasonic wave used in technique, for the ultrasonic wave for the 40W-100W that supersonic generator generates.
Nano chitosan used in the present invention, can be by application No. is 02133792. 6, and the applying date is September 23 in 2002 Day, the method system provided in the Chinese invention patent application file of entitled " a kind of manufacturing method of nano-scale chitosan " It creates.Other raw materials used in the present invention are commercial product.
The advantage of the invention is that：Due to this product artificial skin, has and inhibit bacterial growth and antitumor cell growth Good biological medical characteristics, maintain preferable skin moisture and elasticity, thus can be widely used in burn, scald, The skin-grafting of ulcerous skin, wound hemostasis, antibacterial, anti-inflammatory simultaneously promote cytothesis, accelerate wound healing, film layer is thick, elasticity is good, It is highly practical；And technological process is short, operation is easy, saves equipment.
Specific implementation mode
The acquisition of 1 polypeptide of embodiment
The tissue extraction preparation method of polypeptide, includes the following steps：
1) sea dog cadaver skin is taken, is minced, PBS dissolvings are added papain and trypsin treatment 4h, are subsequently placed in 16h is digested in 4 DEG C of acidic ethanol, it is rear to centrifuge, supernatant spin concentration is taken, is freeze-dried, obtains lyophilized products；
2) size exclusion chromatography detaches：Step 1) obtained freeze-drying object is placed in the acetic acid of a concentration of 2mol/L and is handled Afterwards, it is fitted into 50 chromatographic column of sephadex G, is balanced with the acetic acid of a concentration of 2mol/L, pillar flow velocity 10mL/h, collect chromatography 60 component of peak is lyophilized to get the polypeptide；
It is 4 by volume that the acidic ethanol, which is by ethyl alcohol and the hydrochloric acid of a concentration of 0. 7mol/L,:1 ratio prepare and At；The dosage of the acidic ethanol is：8mL acidic ethanols are added per g skin histologies；
The temperature centrifuged described in step 1) is 4 DEG C, centrifugal force 3000g, time 30min.
The polypeptide is found that 15 its sequence of polypeptide such as SEQ ID NO by detection：Shown in 1-15, has and inhibit bacterium Growth and the effect for keeping skin gloss and elasticity.Therefore, it can be used for the application of artificial skin.
SEQ ID NO：1：KDQQSKCQITFMDLSHNHE；SEQ ID NO：2：WRVMPMFWGLNWWSQKWQ；
SEQ ID NO：3：TCPTHCEDNEWGKYESW；SEQ ID NO：4：RKPHHPWTGSGGSYPE；
SEQ ID NO：5：QWHWNHSFWSDTRH；SEQ ID NO：6：KPKNDDSITETLH；
SEQ ID NO：7：HMKDVDHHPGPPLNPAPMH；SEQ ID NO：8：PMHKSHKIQMIVRWSCRC；
SEQ ID NO：9：FNHFCTTEVFLQQFSRF；SEQ ID NO：10：QDSWLRIQHAEETRHT；
SEQ ID NO：11：VDQYWFPSLGLQRR；SEQ ID NO：12：RVKWSVPHSISRQ；
SEQ ID NO：13：PVEFKHGMSRFFWSIECIM ；
SEQ ID NO：14：LWPQVSHPSDPWCRRLCW；SEQ ID NO：15：TMQIHSGTWCRMTIPTV.
The manufacturing method of artificial skin of the embodiment 2 containing polypeptide
A kind of manufacturing method of artificial skin, is measured in parts by weight, using following proportioning raw materials：Nano chitosan 120, Polypeptide 5, lactic acid 5, organic acid 75, surfactant 30, absolute viscosity are the polyacrylic acid 60 of 4764mpa.s, deionized water 950；And is manufactured in the steps below：The first step, the preparation of A material：Remove 950 parts by weight of ionized water, 75 parts by weight of organic acid, Absolute viscosity is 60 parts by weight of polyacrylic acid of 4764mpa.s, is placed in reaction kettle, in 50 DEG C, 3000 turns/min stirring actions Lower dissolving 1.5 hours becomes the A material that pH value is 3.5-4.0, parts by weight are 1085；Second step, the preparation of B material：After A material are made, The temperature of A material in reaction kettle is kept to be stirred to it at 50 DEG C and at 3000 turns/min, with the time of 30min gradually by 120 weights The Nano chitosan of amount part is added in A material, until Nano chitosan is dissolved almost completely, becomes Nano chitosan solution, referred to as B Material；Third walks, the preparation of Nano chitosan and amino acid or the polymer capsules of lactic acid：After B material are made, by the B in reaction kettle Its temperature is gradually heated up by material under 3000 turns/min stirrings and 40-100W ultrasonic wave collective effects in 1.5 hours from 45 DEG C To 65 DEG C, then, the surfactant of 30 parts by weight is added, continued under aforementioned stirring and ultrasonic wave collective effect, gradually The lactic acid of 5 parts by weight and a kind of polypeptide of 5 parts by weight is added（SEQ ID NO：One kind of 1-15）, do not stopping stirring and surpass Polymerisation is carried out under sound wave effect 2 hours, the temperature in reaction kettle is then gradually increased to 90 DEG C from 65 DEG C with 1.5 hours, And polymerisation is kept at this temperature 1 hour, polymerisation is accused and is finished, and is filtered while hot, that is, it is poly- to obtain light salmon nanoshell Sugar and amino acid or the polymer capsules of lactic acid；4th step, will be at the polymer capsules of Nano chitosan and amino acid or lactic acid Manage into artificial skin prepard from Nano chitosan：By the polymer capsules of Nano chitosan and amino acid or lactic acid, with 40 DEG C of deionization Be washed to neutrality, and shear film forming, then by this film at a temperature of 50 DEG C dry 1.5 hours to get to artificial skin, totally 15 Kind.It is wherein control not add polypeptide.
The activity and Function detection of 3 skin of embodiment
（1）Skin surface moisture content, which scatters and disappears, to be tested
The artificial skin that Example 2 is prepared respectively is cut into 5cm diameter circulars, and it is water-soluble to be immersed in 10% glycerine (glycerine is the moisturizer commonly used in liquid）.Then it removes, applies in human skin 30 minutes.Artificial skin is removed, using After 15 minutes, with CK Electronic Multi-functional skin detectors, 580 types of MPA (Cutometer) detect skin table Face water loses.The skin that exists together not to be covered with any substance equally carries out above-mentioned steps, as a control group (A).The results show that After spreading on skin with the artificial skin for being not added with polypeptide of embodiment 2, lost rate of moisture content of skin reduces 18.7% at this, And it is prepared with embodiment 2 and is added to its moisture loss rate of the artificial skin of polypeptide reduction about 80% or so.In other words It says, skin can be transferred to by glycerine by being added to the artificial skin described in polypeptide with embodiments of the present invention 2 so that the water of skin Dispersion, which is lost, to be reduced, and has preferable skin moisture-keeping characteristic, can prevent skin excessively dry to greatest extent.
（2）Antibacterial skin detects
Artificial skin prepared by embodiment 2 is chosen into 10cm respectively2It is cultivated in human serum culture medium, not with culture medium It does not cross artificial skin preferably, simulates human body environment.By Staphylococcus aureus, hemolytic streptococcus and Pseudomonas aeruginosa according to this field Common cultural method culture is to 5*108CFU/mL then adds and takes 1 to drip to skin surface respectively, and 37 degrees Celsius are cultivated 5 days.It sees It is as follows to examine result：
4 skin tension of embodiment is tested
Using male adult skin as positive control, 15 kinds of skins of polypeptide are added to using in embodiment 2 as experiment pair As using the skin of the embodiment 2 more than no addition as instruction, by the skin tension measurement method of this field routine, measuring The surface tension of this skin in 17.It was found that there is no the skin for adding polypeptide than normal adult skin surface in embodiment 2 Power is small by about 10%, and 15 kinds of its surface tension of skin for being added to polypeptide are more left than the surface tension increase about 20% of adult skin The right side shows preferable skin effects.
Artificial skin based on the above present invention has preferable application prospect.
Sequence table
110 ＞ of ＜ care for silver-colored phoenix
A kind of artificial skins of high activity of 120 ＞ of ＜
〈210〉1
〈211〉 19
〈212〉PRT
213 ＞ artificial sequences of ＜
KDQQSKCQITFMDLSHNHE；
〈210〉2
〈211〉 18
〈212〉PRT
213 ＞ artificial sequences of ＜
WRVMPMFWGLNWWSQKWQ；
〈210〉3
〈211〉 17
〈212〉PRT
213 ＞ artificial sequences of ＜
TCPTHCEDNEWGKYESW；
〈210〉4
〈211〉 16
〈212〉PRT
213 ＞ artificial sequences of ＜
RKPHHPWTGSGGSYPE；
〈210〉5
〈211〉 14
〈212〉PRT
213 ＞ artificial sequences of ＜
QWHWNHSFWSDTRH；
〈210〉6
〈211〉 13
〈212〉PRT
213 ＞ artificial sequences of ＜
KPKNDDSITETLH；
〈210〉7
〈211〉 19
〈212〉PRT
213 ＞ artificial sequences of ＜
HMKDVDHHPGPPLNPAPMH；
〈210〉8
〈211〉 18
〈212〉PRT
213 ＞ artificial sequences of ＜
PMHKSHKIQMIVRWSCRC；
〈210〉9
〈211〉 17
〈212〉PRT
213 ＞ artificial sequences of ＜
FNHFCTTEVFLQQFSRF；
〈210〉10
〈211〉 16
〈212〉PRT
213 ＞ artificial sequences of ＜
QDSWLRIQHAEETRHT；
〈210〉11
〈211〉 14
〈212〉PRT
213 ＞ artificial sequences of ＜
VDQYWFPSLGLQRR；
〈210〉12
〈211〉 13
〈212〉PRT
213 ＞ artificial sequences of ＜
RVKWSVPHSISRQ；
〈210〉13
〈211〉 19
〈212〉PRT
213 ＞ artificial sequences of ＜
PVEFKHGMSRFFWSIECIM；
〈210〉14
〈211〉 18
〈212〉PRT
213 ＞ artificial sequences of ＜
LWPQVSHPSDPWCRRLCW；
〈210〉15
〈211〉 17
〈212〉PRT
213 ＞ artificial sequences of ＜
TMQIHSGTWCRMTIPTV；

Claims (3)

1. a kind of manufacturing method of artificial skin, which is characterized in that measure in parts by weight, using following proportioning raw materials：Nanometer Chitosan 120, polypeptide 5, lactic acid 5, organic acid 75, surfactant 30, absolute viscosity are the polyacrylic acid 60 of 4764mpa.s, Deionized water 950；And it is manufactured in the steps below：The first step, the preparation of A material：Remove 950 parts by weight of ionized water, organic acid 75 parts by weight, 60 parts by weight of polyacrylic acid that absolute viscosity is 4764mpa.s, are placed in reaction kettle, at 50 DEG C, 3000 turns/min It is dissolved 1.5 hours under stirring action, becomes the A material that pH values are 3.5-4.0, parts by weight are 1085；Second step, the preparation of B material：A After material is made, the temperature of A material in reaction kettle is kept to be stirred to it at 50 DEG C and at 3000 turns/min, with time of 30min by Gradually the Nano chitosan of 120 parts by weight is added in A material, until Nano chitosan is dissolved almost completely, it is molten to become Nano chitosan Liquid, referred to as B are expected；Third walks, the preparation of Nano chitosan and amino acid or the polymer capsules of lactic acid：After B material are made, it will react B material in kettle are under 3000 turns/min stirrings and 40-100W ultrasonic wave collective effects, by its temperature from 45 DEG C in 1.5 hours 65 DEG C are gradually heated to, then, adds the surfactant of 30 parts by weight, is continued in aforementioned stirring and ultrasonic wave collective effect Under, it is gradually added into the lactic acid of 5 parts by weight and a kind of polypeptide of 5 parts by weight：For SEQ ID NO：Any one of 1-15, not Stop carrying out polymerisation under stirring and ul-trasonic irradiation 2 hours, then with 1.5 hours by the temperature in reaction kettle, from 65 DEG C 90 DEG C are gradually increased to, and keeps polymerisation at this temperature 1 hour, polymerisation is accused and finished, and is filtered, that is, is obtained shallow while hot Yellowish pink Nano chitosan and amino acid or the polymer capsules of lactic acid；4th step, by Nano chitosan and amino acid or lactic acid Polymer capsules are processed into artificial skin prepard from Nano chitosan：By the polymer capsules of Nano chitosan and amino acid or lactic acid, use 40 DEG C of deionized water is washed till neutrality, and shears film forming, then by this film at a temperature of 50 DEG C dry 1.5 hours to get to Artificial skin.
2. the artificial skin that method as described in claim 1 is prepared.
3. a kind of polypeptide of artificial skin, which is characterized in that sequence is SEQ ID NO：Shown in 1-15 is any.
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CN106421930A (en) * 2016-11-03 2017-02-22 常州亚环环保科技有限公司 Preparation method of nonirritating artificial skin

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CN104971385A (en) * 2015-08-04 2015-10-14 杨国林 Artificial skin

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