CN105169472B - It is a kind of that there is efficient sterilizing, the artificial skin of high activity - Google Patents
It is a kind of that there is efficient sterilizing, the artificial skin of high activity Download PDFInfo
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- CN105169472B CN105169472B CN201510561471.4A CN201510561471A CN105169472B CN 105169472 B CN105169472 B CN 105169472B CN 201510561471 A CN201510561471 A CN 201510561471A CN 105169472 B CN105169472 B CN 105169472B
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Abstract
The present invention relates to a kind of artificial skin with efficient sterilizing, high activity.The artificial skin has preferable elastic form, has the good biomedical characteristic for suppressing bacterium and growth of tumour cell, it is organized as the polymer of Nano chitosan and chitosan and polypeptide.The artificial skin advantage, which is that the biomedical characteristic of the skin is good, medical value is high, technological process is short, operation is easy, save equipment, reduces cost.With preferable application prospect and application value.
Description
Technical field
The present invention relates to medical ancillary technique field, especially a kind of skin-grafting artificial skin.
Background technology
Artificial skin generally requires as the interim replacement skin of the surface of a wound and meets following function:1st, it is close with human body skin
Flexibility, wetting when can also keep certain form and intensity；2nd, it can be close to the surface of a wound, there is resistance bacterium effect；3rd, it can prevent
Moisture and body fluid are escaped from the surface of a wound；4th, the diffusate from surface of a wound depths can be absorbed, and is not resulted between artificial skin and the surface of a wound
Hydrops；5th, with good biocompatibility；6th, do not occur allergic reaction；7th, it can sterilize and sterilize, and can store, prevent thin
Bacterium infects；8th, pain can be delayed, with certain antibacterial action, promote granulation growth and skin regeneration, promote treatment；9th, preferably
It is transparent, it is able to observe that wound healing situation；Once the 10, occurring trauma surface infestation, it can easily be removed from the surface of a wound, wound not damaged
Face.
All kinds of artificial skins, in clinical practice, method all haves the shortcomings that different aspect and different degrees of, or because of adhesion not
Jail, intensity is too poor；Or because antigenicity is too strong, excitant is big；Or because transparent performance is bad, easy infection, or because hindering autosite skin
Growth, scar is innumerable；Or because raw material sources are difficult, cost is too high；Or because technique is numerous assorted, manufacture is difficult, storage etc. is difficult to again,
Successively it is eliminated." artificial skin's heat " is gradually cooled down, therefore, and present clinical unit is still passed in treatment burn practice using some
System method, causes patient pain, and scar is excessive, the phenomenon such as treatment phase length.
The content of the invention
Present invention aim to overcome the shortcomings of that above-mentioned prior art exists and has some strength and bullet there is provided one kind
Property, permeability is good, can closely be attached with the surface of a wound, the surface of a wound can be observed again, sterile, nontoxic, and no antigen or antigenicity are small, to wound
Face is non-stimulated or excitant is smaller, and has stronger bacteria resistance function；It is safe and convenient to use, the surface of a wound can be protected not infected, do not hindered
Autologous skin and skin-grafting plate is hindered to grow, wound healing, scar is smaller, raw material sources extensively, can manufacture, cost is relatively low,
It is easily long-term to preserve, and just with the manufacture method of the artificial skin of transport and the artificial skin.
It is an object of the invention to：For treatment skin trauma in the urgent need to providing a kind of to suppressing bacterium for patient
Growth, while with preferable active artificial skin；It is a further object to provide produce this artificial skin
Process.
A kind of manufacture method of artificial skin, is measured by weight, using following proportioning raw materials：
And manufactured in the steps below：The first step, the preparation of A material：Remove the parts by weight of ionized water 950, the weight of acetic acid 40
Part, the parts by weight of polyacrylic acid 70 that absolute viscosity is 4764mpa.s, are placed in reactor, make in 48 DEG C, 3500 turns/min stirrings
It is the A material that 4.0, parts by weight are 1060 as pH value with lower dissolving 1.5 hours；Second step, the preparation of B material：After A material are made, protect
The temperature of A material in reactor is held at 55 DEG C and it is stirred under 3500 turns/min, with 30min time gradually by 70 weight
The Nano chitosan of part is added in A material, until Nano chitosan is dissolved almost completely, as Nano chitosan solution, is then added again
Enter chitosan, until chitosan is dissolved almost completely, referred to as B material；3rd step, Nano chitosan and chitosan and lactic acid
The preparation of polymer capsules：After B material are made, by the B material in reactor in 3000 turns/min stirrings and 80W ultrasonic wave collective effects
Under, its temperature is gradually heated to 70 DEG C from 55 DEG C in 1.5 hours, then, the surfactant of 20 parts by weight is added, after
Continue under foregoing stirring and ultrasonic wave collective effect, be gradually added into the lactic acid of 5 parts by weight, do not stopping stirring and ul-trasonic irradiation
Lower progress polymerisation 2 hours, then with 1.5 hours by the temperature in reactor, 91 DEG C are gradually increased to from 70 DEG C, and warm herein
Degree is lower to keep polymerisation 1 hour, and polymerisation is that announcement finishes, and is filtered while hot, that is, obtains light salmon Nano chitosan and chitin
The polymer capsules of glycan and lactic acid；The polymer capsules of Nano chitosan and lactic acid are processed into Nano chitosan by the 4th step
Artificial skin：By Nano chitosan and the polymer capsules of lactic acid, neutrality is washed till with 40 DEG C of deionized water, and shear film forming,
Then this film is dried 1.5 hours at a temperature of 50 DEG C, that is, obtain thickness for 0.05-0.3mm, light brown, translucent receive
The polymer film of rice chitosan and chitosan and lactic acid, i.e. referred to as nanometer artificial skin.
The present invention provides the manufacture method of another artificial skin, measures by weight, using following proportioning raw materials：
And manufactured in the steps below：The first step, the preparation of A material：Remove the parts by weight of ionized water 950, the weight of acetic acid 60
Part, the parts by weight of polyacrylic acid 65 that absolute viscosity is 4764mpa.s, are placed in reactor, make in 55 DEG C, 3000 turns/min stirrings
It is the A material that 4.0, parts by weight are 1075 as pH value with lower dissolving 1.5 hours；Second step, the preparation of B material：After A material are made, protect
The temperature of A material in reactor is held at 55 DEG C and it is stirred under 3500 turns/min, with 30min time gradually by 70 weight
The Nano chitosan of part is added in A material, until Nano chitosan is dissolved almost completely, as Nano chitosan solution, is then being added
Enter the parts by weight of chitosan 40, B material are referred to as after same dissolving；The polymer capsules of 3rd step, Nano chitosan and lactic acid
Prepare：After B material are made, by the B material in reactor under 3500 turns/min stirrings and 80W ultrasonic wave collective effects, at 1.5 hours
It is interior that its temperature is gradually heated to 70 DEG C from 55 DEG C, then, the surfactant of 20 parts by weight is added, continued in foregoing stirring
Under ultrasonic wave collective effect, the lactic acid of 5 parts by weight and the polypeptide SEQ ID NO of 10 parts by weight are gradually added into:Any institutes of 1-110
Show, polymerisation is carried out in the case where not stopping stirring and ul-trasonic irradiation 2 hours, then with 1.5 hours by the temperature in reactor,
80 DEG C are gradually increased to from 70 DEG C, and keeps polymerisation at this temperature 1 hour, polymerisation is that announcement finishes, and is filtered while hot, i.e.,
Obtain light salmon Nano chitosan and chitosan and amino acid and the polymer capsules of lactic acid；4th step, by Nano chitosan
Artificial skin prepard from Nano chitosan is processed into the polymer capsules of chitosan and polypeptide and lactic acid：By Nano chitosan and amino
The polymer capsules of acid or lactic acid, neutrality is washed till with 40 DEG C of deionized water, and shears film forming, then by this film in 50 DEG C of temperature
Degree is lower to be dried 1.5 hours, that is, obtains a nanometer artificial skin.
This nanometer artificial skin, with translucent film body, in physiological saline, immersion has soft and full elasticity
Form, the characteristic in biomedicine with good suppression bacterium and high activity, while have preferable skin smoothness and
Moisture.
By dry nanometer artificial skin, strictly carry out disinfection on request, then seal, pack, storage is preserved.
Surfactant used can be Tween-20, peregal 0S-10, the efficient Uniform stains of peregal 0S-15, JFZ-1,
JFZ bleeding agents or other fats polyvingl ethers；
Nano chitosan used, for the Nano chitosan product with following physical and chemical index：Powder granule>80 mesh, powder
Volume density is 0.56-0.62g/ml, powder moisture San 5.6%, and the granularity of Nano chitosan particle is 20-60nm, heavy metal
Impurity content：Lead<0.lmg/kg, mercury<0.lmg/kg, arsenic<0.2mg/kg, dissolves in weak acid, diluted acid or six acetone, sterile；
The chitosan manufactures the chitosan of rank for skin commonly used in the art, sterile.
Ultrasonic wave used in technique, is the ultrasonic wave for the 40W-100W that supersonic generator is produced.
Nano chitosan of the invention used, can be by Application No. 02133792.6, and the applying date is September in 2002 23,
Method manufacture provided in the Chinese invention patent application file of entitled " a kind of manufacture method of nano-scale chitosan "
Out.Other raw materials used of the invention are commercially available prod.
The advantage of the invention is that：Due to this product artificial skin, with bacteria growing inhibiting and good biomedicine
Characteristic, maintains preferable skin moisture and elasticity, therefore can be widely used in burn, scald, the skin-grafting of ulcerous skin,
Wound hemostasis, antibacterial, anti-inflammatory simultaneously promotes cytothesis, accelerates wound healing, and its film layer is thick, elasticity is good, practical；And work
Skill flow is short, operation is easy, save equipment.
Embodiment
A kind of manufacture method of the artificial skin of embodiment 1
Measure by weight, using following proportioning raw materials：
And manufactured in the steps below：The first step, the preparation of A material：Remove the parts by weight of ionized water 950, the weight of acetic acid 40
Part, the parts by weight of polyacrylic acid 70 that absolute viscosity is 4764mpa.s, are placed in reactor, make in 48 DEG C, 3500 turns/min stirrings
It is the A material that 4.0, parts by weight are 1060 as pH value with lower dissolving 1.5 hours；Second step, the preparation of B material：After A material are made, protect
The temperature of A material in reactor is held at 55 DEG C and it is stirred under 3500 turns/min, with 30min time gradually by 70 weight
The Nano chitosan of part is added in A material, until Nano chitosan is dissolved almost completely, as Nano chitosan solution, is then added again
Enter chitosan, until chitosan is dissolved almost completely, referred to as B material；3rd step, Nano chitosan and chitosan and lactic acid
The preparation of polymer capsules：After B material are made, by the B material in reactor in 3000 turns/min stirrings and 80W ultrasonic wave collective effects
Under, its temperature is gradually heated to 70 DEG C from 55 DEG C in 1.5 hours, then, the surfactant of 20 parts by weight is added, after
Continue under foregoing stirring and ultrasonic wave collective effect, be gradually added into the lactic acid of 5 parts by weight, do not stopping stirring and ul-trasonic irradiation
Lower progress polymerisation 2 hours, then with 1.5 hours by the temperature in reactor, 91 DEG C are gradually increased to from 70 DEG C, and warm herein
Degree is lower to keep polymerisation 1 hour, and polymerisation is that announcement finishes, and is filtered while hot, that is, obtains light salmon Nano chitosan and chitin
The polymer capsules of glycan and lactic acid；The polymer capsules of Nano chitosan and lactic acid are processed into Nano chitosan by the 4th step
Artificial skin：By Nano chitosan and the polymer capsules of lactic acid, neutrality is washed till with 40 DEG C of deionized water, and shear film forming,
Then this film is dried 1.5 hours at a temperature of 50 DEG C, that is, obtain thickness for 0.05-0.3mm, light brown, translucent receive
The polymer film of rice chitosan and chitosan and lactic acid, i.e. referred to as nanometer artificial skin.
It is sterilized, pack preservation.Patient is placed in physiological saline in use, can take out the artificial skin sealed up for safekeeping
Immersion 2 hours, you can revert to soft and full elastic normal condition, for Clinical practice.
The acquisition of the polypeptide of embodiment 2
The tissue extraction preparation method of polypeptide, comprises the following steps：
1) take pineapple to join 1, mince, PBS dissolvings add papain and trypsin treatment 3h, are subsequently placed in 4 DEG C
Acidic ethanol in digest 10h, it is rear to centrifuge, take supernatant spin concentration, be freeze-dried, obtain lyophilized products；
2) size exclusion chromatography is separated：By step 1) obtained freeze-drying thing be placed in concentration be 2mol/L acetic acid in handle
Afterwards, it is fitted into the chromatographic column of sephadex G 50, is balanced using concentration as 2mol/L acetic acid, pillar flow velocity 10mL/h, collects chromatogram
The component of peak 60, freezes, produces the polypeptide；
The hydrochloric acid that it is 0.7mol/L by ethanol and concentration that the acidic ethanol, which is, is 3 by volume:1 proportions are formed
's；The consumption of the acidic ethanol is：8mL acidic ethanols are added per g sea cucumbers；
Step 1) described in the temperature that centrifuges be 4 DEG C, centrifugal force is 3000g, and the time is 30min.
The polypeptide is found that its sequence of 110 polypeptides such as SEQ ID NO by detection：Shown in 1-110, with suppression bacterium
Growth and promotion skin have the effect of more preferable characteristic.It therefore, it can the application for artificial skin.
The manufacture method of artificial skin of the embodiment 3 containing polypeptide
Measure by weight, using following proportioning raw materials：
And manufactured in the steps below：The first step, the preparation of A material：Remove the parts by weight of ionized water 950, the weight of acetic acid 60
Part, the parts by weight of polyacrylic acid 65 that absolute viscosity is 4764mpa.s, are placed in reactor, make in 55 DEG C, 3000 turns/min stirrings
It is the A material that 4.0, parts by weight are 1075 as pH value with lower dissolving 1.5 hours；Second step, the preparation of B material：After A material are made, protect
The temperature of A material in reactor is held at 55 DEG C and it is stirred under 3500 turns/min, with 30min time gradually by 70 weight
The Nano chitosan of part is added in A material, until Nano chitosan is dissolved almost completely, as Nano chitosan solution, is then being added
Enter the parts by weight of chitosan 40, B material are referred to as after same dissolving；The polymer capsules of 3rd step, Nano chitosan and lactic acid
Prepare：After B material are made, by the B material in reactor under 3500 turns/min stirrings and 80W ultrasonic wave collective effects, at 1.5 hours
It is interior that its temperature is gradually heated to 70 DEG C from 55 DEG C, then, the surfactant of 20 parts by weight is added, continued in foregoing stirring
Under ultrasonic wave collective effect, the lactic acid of 5 parts by weight and the polypeptide SEQ ID NO of 10 parts by weight are gradually added into:Any institutes of 1-110
Show, polymerisation is carried out in the case where not stopping stirring and ul-trasonic irradiation 2 hours, then with 1.5 hours by the temperature in reactor,
80 DEG C are gradually increased to from 70 DEG C, and keeps polymerisation at this temperature 1 hour, polymerisation is that announcement finishes, and is filtered while hot, i.e.,
Obtain light salmon Nano chitosan and chitosan and amino acid and the polymer capsules of lactic acid；4th step, by Nano chitosan
Artificial skin prepard from Nano chitosan is processed into the polymer capsules of chitosan and polypeptide and lactic acid：By Nano chitosan and amino
The polymer capsules of acid or lactic acid, neutrality is washed till with 40 DEG C of deionized water, and shears film forming, then by this film in 50 DEG C of temperature
Degree is lower to be dried 1.5 hours, that is, obtains a nanometer artificial skin.
The activity and Function detection of the skin of embodiment 4
(1) skin surface moisture content, which scatters and disappears, tests
The artificial skin that Example 1 and embodiment 3 are prepared, is respectively cut into 5cm diameter circulars, is immersed in 10% sweet
In oil solution (glycerine is conventional NMF).Then remove, apply in human body skin 30 minutes.Artificial skin is removed, then is passed through
Cross after 15 minutes, with CK Electronic Multi-functional skin detectors, the types of MPA 580 (Cutometer) detection skin surface water
Lose.Above-mentioned steps are equally carried out with the skin that exists together for not being covered with any material, as a control group (A).As a result show, with embodiment
1 artificial skin is spread on after skin, the lost speed reduction 21.2% of the moisture content of skin at this, and prepared with embodiment 3
Its moisture loss speed of artificial skin is fluctuated in the range of reducing about 75%.Whole result is similar.In other words, with the present invention
Embodiment 3 described in artificial skin glycerine can be transferred to skin so that the reduction of the moisture loss of skin, with preferable skin
Skin humectant properties, can prevent skin from excessively drying to greatest extent.
(2) antibacterial skin is detected
Artificial skin prepared by embodiment 1 and embodiment 3 is chosen into 10cm respectively2Cultivated in human serum culture medium,
Preferably not having artificial skin with culture medium, human body environment is simulated.By Staphylococcus aureus, hemolytic streptococcus and Pseudomonas aeruginosa
According to cultural method culture commonly used in the art to 108CFU/mL, then adds and takes 1 to drip to skin surface, 37 degrees Celsius of trainings respectively
Support 3 days.Observe result as follows：
(3) the therapeutic effect experiment of skin
Case selection：Select dermatosis patient 110, wherein male 50, women 60, age 20-75 Sui, average year
Age 47.5. ratio distribution such as following table：Burn, scald patient.Artificial skin prepared by a kind of polypeptide of each use is treated.
| Wounded patient | Number of cases | Recover number of cases completely | Significantly improve number of cases | Invalid number of cases |
| Burn | 75 | 70 | 5 | 0 |
| Scald | 35 | 31 | 4 | 0 |
Recover to refer to after treatment completely, skin and autologous skin difference are not highly significants, with preferable function.
Significantly improve and refer to after treatment, skin has certain difference with autologous skin, but can exercise normal work(
Energy.
(4) the stretch-proof experiment of artificial skin
Using the measuring method of the conventional skin properties in this area, hardness, tensile strength, the rigidity of artificial skin are measured,
As a result it is as follows：
Result more than, which can be seen that the artificial skin of the invention provided, has preferable antagonistic property, while also having
There is more preferable elasticity, be suitable for the treatment of skin, with more wide application prospect.
Sequence table
The ＞ poplars state woodss of ＜ 110
The ＞ of ＜ 120 are a kind of to have efficient sterilizing, the artificial skin of high activity
〈210〉1
〈211〉 19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
SRRQRWHWSSRMMRRKPCE；
〈210〉2
〈211〉 18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
HPDWAVIVWIRGQRQMSQ；
〈210〉3
〈211〉 16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
WIVPFWCIQFWQKMNR；
〈210〉4
〈211〉 14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
QFSMHLPQHRYNAP；
〈210〉5
〈211〉 19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
EWCHPKMNSFDQHWCTPHR；
〈210〉6
〈211〉 18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
SPVQARHFMMSDYNGGDD；
〈210〉7
〈211〉 16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
LHYNWPLWCFQGSVRH；
〈210〉8
〈211〉 14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
ASNQFVCWHACKMW；
〈210〉9
〈211〉 13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
CATHCQCFEHWGR；
〈210〉10
〈211〉 19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
RTMQPWPWPVMSHAEQAPR；
〈210〉11
〈211〉 18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
RHNMDRYHFHKVQPAHWW；
〈210〉12
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
KSDERAWMTWRSVWTL；
〈210〉13
〈211〉 14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PVAMDMQQHKRTLF；
〈210〉14
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
YWNMQSCWRQRWHQLPHKT；
〈210〉15
〈211〉 18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
IPFARLTSFGNLIFDHRC；
〈210〉16
〈211〉 16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
RMSQHGQYLWAEWHFE；
〈210〉17
〈211〉 14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
KNKSHGGAAEPKPD；
〈210〉18
〈211〉 13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
KQQASEFHFEWCW；
〈210〉19
〈211〉 19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
FGPNQMQWNPWPSALKSRN；
〈210〉20
〈211〉 18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
GETNADQQVYFIWPSMGS；
〈210〉21
〈211〉 16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
LYWRMDKSQPSLSALY；
〈210〉22
〈211〉 14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
LWWSFSNSWIQDGP；
〈210〉23
〈211〉 13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PMKRARSGYVHTA；
〈210〉24
〈211〉 19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PWQGWIIYRTFHYWIWYKL；
〈210〉25
〈211〉 18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
AIPMPWHSTPPGPDQAQN；
〈210〉26
〈211〉 16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
FVFWYAAPHMPEPGYH；
〈210〉27
〈211〉 14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
SQWIRSPGCQWCPQ；
〈210〉28
〈211〉 13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
SGWSDRIKPNWHA；
〈210〉29
〈211〉 19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
TQQGWSLQDLCAKAQHYNV；
〈210〉30
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
WWKWLLMQNHIHPQKSQF；
〈210〉31
〈211〉 16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
KKSRVCLGKPLSVQWM；
〈210〉32
〈211〉 13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
WIDCQYFQTWKPR；
〈210〉33
〈211〉 19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
REHVRPSSEKFFQLHKKVW；
〈210〉34
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
RNGIVWNRDHHHKHGYHR；
〈210〉35
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
NSHMVHIVPNFYMDQL；
〈210〉36
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
DSPAETWGQSDQCT；
〈210〉37
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PQHSNHEQQSSAF；
〈210〉38
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
GYQQPAFLEWGWPW；
〈210〉39
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
CRQKRRHGHDRSR；
〈210〉40
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
SVCPWPTNMQEEITQCPFP；
〈210〉41
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PRMTPYHPHSENQNFLSN；
〈210〉42
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
QPTGQEIPSWKDMVWH；
〈210〉43
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
VVREFQCTQSWPQQWRWIR；
〈210〉44
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
HPIWWPWNFHMRQPQKNP；
〈210〉45
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
QRLYVPQMAQQAPPRS；
〈210〉46
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
QWQTIWLFRRPMSL；
〈210〉47
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
MTCMIVFPHCCCW；
〈210〉48
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
VGWTIHLTGCTEPAINIIW；
〈210〉49
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
DWSCWPNRFWLATADTTE；
〈210〉50
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
CSPLKTESAFWAQICS；
〈210〉51
〈211〉 14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
MSDQPKNCPPGVHA；
〈210〉52
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
TYSQPTLFKKCIS；
〈210〉53
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
FVSNQQRQPMPSSMDWQNF；
〈210〉54
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
RRPFKSVRIHWLMSSNFQ；
〈210〉55
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
ASMTHFEKMCRSWCSC；
〈210〉56
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
KTNPPRNYDGVEVN；
〈210〉57
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
LEVCCCHWWPRWL；
〈210〉58
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
RMHFSHILHPRLFPAWWTP；
〈210〉59
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PGIFRQIPQRYIDPVKWR；
〈210〉60
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
IYLSHPTQRMQHQCML；
〈210〉61
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
HFEQHDNKVMTCRK；
〈210〉62
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PMVREMQEGSNAD；
〈210〉63
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
VDYWHNQVWSCRVTWWHHF；
〈210〉64
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
WKNVGEPLHENCTQEHSH；
〈210〉65
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
QVMHGTRQAPFWDNNS；
〈210〉66
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
NSHLTKSIFFPQH；
〈210〉67
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
FVDEHAQTQTNREWHKFAL；
〈210〉68
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
QMRIQWNIRPKWPHRHYH；
〈210〉69
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
WFPCKQQNDKQTPPMN；
〈210〉70
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
RWSWIGCREEMWAR；
〈210〉71
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
FQQERFIQQCGHM；
〈210〉72
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
QVGPICTRRWEFAYMERWY；
〈210〉73
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
RKTCAYNFIINCVSHSTR；
〈210〉74
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
WNQPNIIQPGHFRKTT；
〈210〉75
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PKMHCRTGNPNHRV；
〈210〉76
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
KPLRKLPTMHQHR；
〈210〉77
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PPHPYYPYPSFVPSLACSK；
〈210〉78
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
HSESICRTHKHPHMSVAT；
〈210〉79
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
MPPSIWSWHNPHGQWN；
〈210〉80
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
AEHPRFGIMIQTIA；
〈210〉81
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
WWFMDPGKHGDHR；
〈210〉82
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
FYRHFGDPFRQSVRQVKPW；
〈210〉83
〈211〉 18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
FMSVIVLRSRVHWSEAID；
〈210〉84
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
MSVRFRLGIQGEHPWW；
〈210〉85
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PEIQMQKVDPDHWF；
〈210〉86
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
YRRQHAHWDPCRG；
〈210〉87
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
HEWDQMRRHLDTPAKKDFQ；
〈210〉88
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
SKEIVCPVFEHMAWNEQH；
〈210〉89
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
SHIGQRQPCQQSPDSQ；
〈210〉90
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
KRWNWVQMTHLPWR；
〈210〉91
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
LYPDLPWWQIQWP；
〈210〉92
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
QVNHSHVRRKKPRYMAETG；
〈210〉93
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
NGNTDARRPRENQAILIS；
〈210〉94
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
SLQDVLYYLTMANITM；
〈210〉95
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
KTWYNVLLDPPPM；
〈210〉96
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
SHCSEIGRFNEQVQMIWRW；
〈210〉97
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
EQAWFHRWHEEMLWTRPM；
〈210〉98
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
HWVDLSSWCAGDPQLP；
〈210〉99
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
FRKVAMWWSCHQSH；
〈210〉100
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
LCVHQCNPRIKSM；
〈210〉101
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
IRWFLRISQWVQHKRHYWS；
〈210〉102
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
FHPERHKPVAGWIPPHWN；
〈210〉103
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
REIAGMPQTTWSHWDS；
〈210〉104
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
EQWIVGQPQHRPIE；
〈210〉105
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PFVQQMRWSIFLH；
〈210〉106
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
EQLDEEWITRQGCGIHDPD；
〈210〉107
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
HVMGQTEQSRRNNWGHSP；
〈210〉108
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
PYPECCIHQRPWNDPR；
〈210〉109
〈211〉14
〈212〉PRT
The ＞ artificial sequences of ＜ 213
AKHWQAHKVMYVHR；
〈210〉110
〈211〉13
〈212〉PRT
The ＞ artificial sequences of ＜ 213
KIAQPPEYQSLAR；
Claims (4)
1. a kind of manufacture method of artificial skin, it is characterised in that：Preparation process is as follows：The first step, the preparation of A material：Remove from
The sub- parts by weight of water 950, the parts by weight of acetic acid 60, absolute viscosity are the 4764mpa.s parts by weight of polyacrylic acid 65, are placed in reactor
In, dissolved 1.5 hours under 55 DEG C, 3000 turns/min stirring actions, be the A material that 4.0, parts by weight are 1075 as pH value；The
Two steps, the preparation of B material：After A material are made, the temperature of A material in reactor is kept to be stirred under 3500 turns/min at 55 DEG C and to it
Mix, gradually added the Nano chitosan of 70 parts by weight in A material with 30min time, until Nano chitosan is dissolved almost completely,
As Nano chitosan solution, the parts by weight of chitosan 40 then are being added, B material are referred to as after same dissolving；3rd step, nanometer
The preparation of the polymer capsules of chitosan and lactic acid：After B material are made, by the B material in reactor in 3500 turns/min stirrings and 80W
Under ultrasonic wave collective effect, its temperature is gradually heated to 70 DEG C from 55 DEG C in 1.5 hours, then, 20 parts by weight are added
Surfactant, continue it is foregoing stirring and ultrasonic wave collective effect under, be gradually added into the lactic acid and 10 parts by weight of 5 parts by weight
Polypeptide SEQ ID NO:Shown in 1-110 is any, polymerisation is carried out in the case where not stopping stirring and ul-trasonic irradiation 2 hours, connect
The temperature in reactor with 1.5 hours, 80 DEG C be gradually increased to from 70 DEG C, and keep polymerisation at this temperature 1 hour,
Polymerisation is that announcement finishes, and is filtered while hot, i.e., acquisition light salmon Nano chitosan and chitosan and amino acid and lactic acid is poly-
Compound utricule；The polymer capsules of Nano chitosan and chitosan and polypeptide and lactic acid are processed into nanoshell and gathered by the 4th step
Sugared artificial skin：By Nano chitosan and the polymer capsules of amino acid or lactic acid, neutrality is washed till with 40 DEG C of deionized water, and
Film forming is sheared, then this film is dried 1.5 hours at a temperature of 50 DEG C, that is, obtains a nanometer artificial skin.
2. the artificial skin that claim 1 methods described is prepared.
3. the artificial skin described in claim 2 is preparing the application in being used to treat the medicine of skin burn or scald.
4. a kind of polypeptide for artificial skin, it is characterised in that：Its amino acid sequence such as SEQ ID NO:Any one of 1-110 institutes
Show.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510561471.4A CN105169472B (en) | 2015-09-06 | 2015-09-06 | It is a kind of that there is efficient sterilizing, the artificial skin of high activity |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510561471.4A CN105169472B (en) | 2015-09-06 | 2015-09-06 | It is a kind of that there is efficient sterilizing, the artificial skin of high activity |
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| CN105169472B true CN105169472B (en) | 2017-10-20 |
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| CN201510561471.4A Active CN105169472B (en) | 2015-09-06 | 2015-09-06 | It is a kind of that there is efficient sterilizing, the artificial skin of high activity |
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Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1795935B (en) * | 2004-12-28 | 2010-09-29 | 广汉恒宇新材料有限公司 | Artificial skin prepared from Nano chitosan and preparing method |
| CN104292305B (en) * | 2014-01-17 | 2017-06-09 | 河南科技大学 | A kind of polypeptide, preparation method and applications |
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Inventor after: Zhang Lianbo Inventor after: Zhang Guang Inventor after: Liu Tianji Inventor before: Yang Guolin |
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Effective date of registration: 20170925 Address after: 130000 Changchun Qianjin Street, Jilin, No. 2699 Applicant after: Jilin University Address before: 100000 Beijing city Shijingshan District Street iron new house 34 ranked No. 7 Applicant before: Yang Guolin |
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