CN105360289A - Glucanase titanium silicon agent for preventing papaya anthracnose - Google Patents

Abstract

The invention discloses a glucanase titanium silicon agent for preventing papaya anthracnose, which belongs to the technical field of plant disease biological control, and uses bacillus licheniformis and bacillus mucilaginosus, adopts spherical shell clustering 80 nano indium tin oxides (ITO2) as a catalyst, prepares antibiotics containing glucanase and chitinase through a catalytic fermentation method under the conditions of 450nm:660nm in optical wavelength and 8:2 in rate and 2500 lumens in illuminance by culture, stimulates papaya to produce endogenous origin antibiotics after being used, and can prevent and resist the papaya anthracnose which is caused by Colletotrichum nigrum, Gloeosporium piperatum and Colletotrichum capsici. The glucanase titanium silicon agent for preventing the papaya anthracnose uses natural materials of rice, brown sugar and salt, is formed by adopting probiotics to ferment, is adsorbed without residue by plants after being used, and is a biological type non-toxic no-residue organic prevention scheme for preventing the papaya anthracnose.

Description

A kind of dextranase silicon titanium mixture preventing and treating papaya anthracnose

Technical field

The present invention relates to a kind of dextranase silicon titanium mixture preventing and treating papaya anthracnose, prevent and treat for black thorn dish spore bacterium, capsicum dish long spore bacterium, the fruit of the microbial papaya of capsicum clump thorn dish spore, the anthracnose of blade, the function that the present invention relates to belongs to the technical field of agricultural disease control.

Background technology

The pathogen of papaya anthracnose is divided into the black thorn dish spore bacterium causing fruit rot, the long spore bacterium of capsicum dish, cause leaf, the capsicum clump thorn dish spore bacterium of petiole anthracnose, one of major reason causing papaya post-harvest fruits to rot, be by the pathogen of Fruit Anthracnose when field latent infection in fruit, field and after gathering the Chinese olive phase generally do not fall ill, once fruit enters the stage of yellow ripeness, the anthracnose of hiding will be fallen ill and be produced harm, cause fruit in storage, transport, serious rotting in sales process, in actual mechanical process, hide because of anthrax-bacilus and to be difficult to find in Chinese olive, in Chinese olive postharvest storage period because pathogen is via aqueous vapor, the reasons such as the friction between fruit, cause mutual infection, thus morbidity is started in the stage of yellow ripeness, the lighter produces scab at fruit, cause time serious and all rot, cause loss economically, pawpaw tree in field is because the factor of weather, especially at the beginning of last month of spring in winter, during the time of the year when autumn changes into winter, pathogen morbidity causes the stem of papaya to rot, the root of pawpaw is penetrated into time serious, cause the stem of papaya, root rot, cause whole strain dead, disease control at present after papaya Chinese olive is gathered, main dependence chemical agent and ripener carry out anticorrosion process of accelerating the ripening, pesticide control common in control in field, Prochloraz, carbendazim, Bravo, thiophanate methyl, the chemical agents such as Mancozeb are prevented and treated, but these chemical bactericide of a large amount of uses brings more and more serious threat to the health of human body and environment, people are concerned about food-safety problem day by day, nontoxic, in non-harmful commodity, these chemical bactericides must not be required and have been detected, the technician of domestic and international control of plant disease all make great efforts research new adopt safely and effectively after with the anthracnose prevention and controls in planting process.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of dextranase silicon titanium mixture preventing and treating papaya anthracnose, mainly applying rice husk becomes thoroughly decomposed containing a large amount of water-soluble silicon elements, coordinate bacillus licheniformis, colloid bacillus cereus, the photodissociation culture medium of bacillus subtilis, the plant endogenesis source antibiotic of dextranase is obtained after fermentation, its function is directly act on β (1.3) glucan in hydrolysis pathogen cells wall, thus cause lysis and the death of pathogen, Water-soluble silicon fertilizer is the essential elements required for papaya growth, papaya can form silicified cell after absorbing siliceous fertilizer in vivo, make stem, leaf, the cells of superficial layer wall thickening of fruit, cuticula increases, form one firm protective layer, that prevents and treats pathogen infects attachment, titanium elements can strengthen the photosynthesis of plant under the low light level, increase the nutrition conversion capability of papaya, promote stem's lignin and cellulosic formation speed, form natural diaphragm, chitin can bring out the generation of the chitinase in papaya body, the antibody producing oneself resists infecting of pathogen, by the acting in conjunction of above-mentioned various enzyme and element, to the deactivation of pathogen, prevent from infecting, the solution of systemic control anthracnose is played in self-protection, reach green, non-polluted planting, the target of storage.

The present invention is that to solve the problems of the technologies described above the technical scheme adopted be a kind of dextranase silicon titanium mixture preventing and treating papaya anthracnose, its material mixture ratio and processing step as follows:

1, material:

(1), material I enriched medium: paddy becomes thoroughly decomposed leaching liquor 82%, brown sugar 15%, salt 2%, calcium carbonate 0.5%, zinc sulfate 0.2%, manganese sulfate 0.17%, magnesium sulfate 0.2%, ferrous sulfate 0.02%;

(2), material II bacterial classification: bacillus licheniformis 8000IU is a, and colloid bacillus cereus 8000IU is a;

(3), material III photochemical catalyst: it is a that shell gathers ball 80 nano-ITO 2;

(4), material IV thickener: shitosan is a;

2, processing step:

(1), by after paddy ammonification, add flooding 48H after becoming thoroughly decomposed via hot fermentation groove, filtration residue obtains paddy and to become thoroughly decomposed leaching liquor;

(2), further by paddy leaching liquor add the other materials of enriched medium, after being uniformly mixed, put into high-temperature sterilization pot, carry out sterilizing with 120 DEG C;

(3), further the red enriched medium of completing steps 2 is placed to be cooled after 25 DEG C, be placed in the fermentation cauldron of glass, inoculation material II bacterial classification;

(4), further add the photochemical catalyst of material III, start illumination fermentation 7d after stirring, irradiating optical wavelength between yeast phase is 450nm:660nm, ratio is 8:2, the light of illumination 2500 lumen, and fermentation temperature maintains 25 DEG C-28 DEG C, fermented filtration residue, obtains suspension for subsequent use;

(5), further material IV thickener is added in the suspension of completing steps 4, obtain transparent thick liquid after stirring, be a kind of dextranase silicon titanium mixture preventing and treating papaya anthracnose;

Advantage of the present invention:

The present invention contrasts the advantage of prior art, the present invention is mainly by dextranase, chitinase, silicon, the systemic control method of the papaya anthracnose that titanium is polymerized, it is the biological nature utilizing papaya, thus bring out the antibacterial ability of crop itself, at papaya growing period, with the protective capability of oneself, resist intrusion and the morbidity of Anthracnose Pathogen body, the present invention exists in gel form mode, directly spread upon on the crust of fruit after papaya Chinese olive is adopted, directly the existence of pathogen is destroyed, reduce the development of pathogen, reach Chinese olive storage, transport, in the approach sold, reduce rotting due to the initiation of anthrax pathogen, solve the Bravo that current papaya usually uses in plantation with storage, carbendazim, Prochloraz, the use of the agricultural chemicals such as Mancozeb, the soil pollution caused and the problem of residues of pesticides, the advantage that the present invention separately has is that the raw material that obtains and technological process are simple, with low cost, problem that is residual and soil pollution is not produced after using, the mechanism of effect is that the interior raw antibiotic of induction papaya itself and the principle of enhancing antibody reach disease-resistant effect, the resistance to the action of a drug of pathogen is caused after can not producing use, it is a kind of product of worth large-scale promotion application, solve the plantation of current papaya, to the problem that agricultural chemicals relies in storage.

Accompanying drawing explanation

A kind of dextranase silicon titanium mixture preventing and treating papaya anthracnose of Fig. 1

detailed description of the invention and case

coordinate actual use case that characteristic of the present invention is described below

1, case one: Zhangjiagang, Jiangsu papaya plantation farm, utilize greenhouse gardening papaya 60 mu, in field planting in September, 2014, find that stem rots in March, 2015, the phenomenon of plant lodging, Chinese olive finds wheel line shape scab, after cultivating, determine it is infect the papaya anthracnose caused by black thorn dish spore bacterium, the long spore bacterium of capsicum dish and capsicum clump thorn dish spore bacterium; The dextranase silicon titanium mixture 500 times of liquid applying papaya anthracnose of the present invention spray the papaya in whole strain, spray after 10 once, do not flow dense rotting in the sick portion drying finding to be infected by the end of April, new stem young leaves regeneration, May, new stem grew, affected part degree of lignification is high, robust plant, plucks Chinese olive at the bottom of May, and user's 2000 times of liquid spread upon whole fruit storage, after mid-June Chinese olive yellow maturity, fruit dehydration drying has no rotten phenomenon;

2, case two: farm, the suburbs, Changsha, application simple plastic greenhouse plants red prince wife's papaya 20 mu, in field planting in March, 2015 papaya seedling, find that the aerial part of papaya occurs that the scab of wheel line shape does not find ulcer shape in June, after Bacteria Culture, determine it is by the microbial papaya anthracnose of capsicum clump thorn dish spore, apply dextranase silicon titanium mixture of the present invention 500 times of liquid to spray and spray in the papaya 1000 Bei Yezheng garden of whole strain, continued to spray once every 15 days backward, the sick portion of having an attack of one's old illness is checked when June, find that the phenomenon of wheel line shape disappears, other not ill plant do not find symptom,

By above actual in the application in land for growing field crops, when the morbidity of papaya anthracnose is serious, take the ratio of high concentration to treat, early stage adopts the ratio of low solubility to treat, and serves the effect suppressing the activity of germ to reach healing, after Chinese olive is plucked before storage, be applied in fruit surface by low concentration proportioning, antiseptic effect is good, checks the quality index of the ripening fruits of papaya, the sugariness of papaya on the market, between 9-11 degree, uses papaya sugariness of the present invention at 12-14 degree.There is fruital taste that papaya is original in addition, hardness ratio has more hardness on the market, be applicable to long-distance transport, use the dextranase silicon titanium mixture of control papaya anthracnose of the present invention, except preventing and treating except the anthracnose of papaya, separately can increase the sugariness of fruit, colourity and the local flavor of itself with and the resistance of crop own, be the solution of a kind of green non-poisonous papaya anthracnose control be worthy to be popularized.

Although the present invention discloses as above with preferred embodiment; so itself and be not used to limit the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; when doing a little amendment and perfect, therefore protection scope of the present invention is when being as the criterion of defining with claims.

Claims (2)

1. prevent and treat the dextranase silicon titanium mixture of papaya anthracnose for one kind, it is characterized in that by bacillus licheniformis, colloid bacillus cereus, ball 80 nano-ITO 2 is gathered for photochemical catalyst by shell, via the optical wavelength of 450nm:660nm, ratio is 8:2, the light-illuminating fermentation of illumination 2500 lumen, produces the antibiotic containing dextranase, chitinase.

2. a kind of dextranase silicon titanium mixture preventing and treating papaya anthracnose as claimed in claim 1, is characterized in that adopting following materials and process step to make:

1), material: material I enriched medium, paddy becomes thoroughly decomposed leaching liquor 82%, brown sugar 15%, salt 2%, calcium carbonate 0.5%, zinc sulfate 0.2%, manganese sulfate 0.17%, magnesium sulfate 0.2%, ferrous sulfate 0.02%; Material II bacterial classification, bacillus licheniformis 8000IU is a, and colloid bacillus cereus 8000IU is a; Material III photochemical catalyst, it is a that shell gathers ball 80 nano-ITO 2; Material IV thickener, shitosan is a;

2), processing step:

(1), by after paddy ammonification, add flooding 48H after becoming thoroughly decomposed via hot fermentation groove, filtration residue obtains paddy and to become thoroughly decomposed leaching liquor;

(2), further by paddy leaching liquor add the other materials of enriched medium, after being uniformly mixed, put into high-temperature sterilization pot, carry out sterilizing with 120 DEG C;

(3), further the red enriched medium of completing steps 2 is placed to be cooled after 25 DEG C, be placed in the fermentation cauldron of glass, inoculation material II bacterial classification;

(4), further add the photochemical catalyst of material III, start illumination fermentation 7d after stirring, irradiating optical wavelength between yeast phase is 450nm:660nm, ratio is 8:2, the light of illumination 2500 lumen, and fermentation temperature maintains 25 DEG C-28 DEG C, fermented filtration residue, obtains suspension for subsequent use;

(5), further material IV thickener is added in the suspension of completing steps 4, obtain transparent thick liquid after stirring, be a kind of dextranase silicon titanium mixture preventing and treating papaya anthracnose.