CN105360183A - Traditional Chinese medicine spraying agent for hospital sterilization and disinfection and preparation method thereof - Google Patents

Traditional Chinese medicine spraying agent for hospital sterilization and disinfection and preparation method thereof Download PDF

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CN105360183A
CN105360183A CN201510828680.0A CN201510828680A CN105360183A CN 105360183 A CN105360183 A CN 105360183A CN 201510828680 A CN201510828680 A CN 201510828680A CN 105360183 A CN105360183 A CN 105360183A
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王清源
李成香
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Abstract

The invention discloses a traditional Chinese medicine spraying agent for hospital sterilization and disinfection and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine spraying agent is used for solving the problem of large side effects of a conventional disinfectant and is composed of the following traditional Chinese medicine raw materials in parts by weight: 10-15 parts of herba houttuyniae, 15-18 parts of cornus officinalis, 5-8 parts of cicada slough, 15-20 parts of herba ephedra, 3-5 parts of cyrtomium fortunei, 10-18 parts of szechwan chinaberry fruit, 15-22 parts of honeysuckle, 3-8 parts of mulberry leaves, 2-5 parts of rhizoma anemarrhenae, 8-12 parts of pomegranate peel, 8-12 parts of radix saposhnikoviae, 10-15 parts of root of Chinese pulsatilla, 5-10 parts of dandelion, 3-6 parts of radix sophorae flavescentis, 2-10 parts of rhizoma cimicifugae, 10-15 parts of coptis chinensis, 3-5 parts of radix clematidis, and 1-3 parts of belamcanda chinensis. The preparation method comprises the steps of grinding, alcohol extraction, water extraction and mixing. The traditional Chinese medicine spraying agent is suitable for all personnel intensive areas such as medical, hygiene and health-care institutions and the like.

Description

A kind of medicinal herb spray for hospital's sterilizing and preparation method thereof
Technical field
The present invention relates to a kind of medicinal herb spray for hospital's sterilizing and preparation method thereof, belong to technical field of traditional Chinese medicines.
Background technology
Air is one of main media of transmission, air sterillization is that cut-out respiratory infectious disease route of transmission is the most effective, the most direct method, sterilizing method conventional is at present that disinfectant sprays, chemical method and the Ultraviolet radiation such as stifling, the physical methods such as ozone, wherein chemical method adopt the most irritant smell of medicament or to skin have stimulate damage, Long-Time Service can affect health to some extent, Ultraviolet radiation is by fluorescent tube intensity, cleanliness factor, ambient temperature and humidity, shelter and distance impact, and ultraviolet is large to skin lethality, the side reactions such as ultraviolet ophthalmia can be caused.Therefore, two kinds of methods all should not be used for the air sterillization of the very large hospital of flow of the people.
Goal of the invention
The object of the present invention is to provide a kind of medicinal herb spray for hospital's sterilizing, production cost is low, and safety non-toxic is non-stimulated, and bactericidal effect is good.
Invention also provides a kind of preparation method of the medicinal herb spray for hospital's sterilizing.
The described medicinal herb spray for hospital's sterilizing, is made up of the Chinese medicine material of following weight portion:
Cordate houttuynia 10-15 part, dogwood 15-18 part, cicada slough 5-8 part, Chinese ephedra 15-20 part, rhizome of cyrtomium 3-5 part, Fructus meliae toosendan 10-18 part, honeysuckle 15-22 part, mulberry leaf 3-8 part, wind-weed 2-5 part, granatum 8-12 part, windproof 8-12 part, Chinese pulsatilla 10-15 part, dandelion 5-10 part, kuh-seng 3-6 part, rattletop 2-10 part, coptis 10-15 part, root of Chinese clematis 3-5 part, blackberry lily 1-3 part.
Preferably for the medicinal herb spray of hospital's sterilizing, be made up of the Chinese medicine material of following weight portion:
Cordate houttuynia 12 parts, dogwood 16 parts, cicada slough 7 parts, 18 parts, Chinese ephedra, rhizome of cyrtomium 4 parts, Fructus meliae toosendan 15 parts, honeysuckle 20 parts, 5 parts, mulberry leaf, the wind-weed 3 parts, granatum 10 parts, windproof 10 parts, the Chinese pulsatilla 12 parts, dandelion 8 parts, kuh-seng 4 parts, rattletop 6 parts, the coptis 13 parts, 4 parts, the root of Chinese clematis, blackberry lily 2 parts.
The preparation method of the described medicinal herb spray for hospital's sterilizing, comprises the following steps:
(1) the Chinese medicine material difference grind into powder getting above-mentioned weight portion is for subsequent use;
(2) get Heartleaf Houttuynia Herb according to the mass ratio of 1:3-6 join mass fraction be soak 8-10 hour in the alcohol of 95% after, it is for subsequent use that cordate houttuynia filtrate is got in centrifugation;
(3) extracting honeysuckle, Fructus meliae toosendan, rattletop, it is add hot reflux 2-3 hour in the alcohol of 90% that the coptis and Ephedra mixing join mass fraction according to the mass ratio of 1:5-10, gets distillate for subsequent use;
(4) dogwood is got, cicada slough, rhizome of cyrtomium, mulberry leaf, the wind-weed, granatum, windproof, the Chinese pulsatilla, dandelion, kuh-seng, the root of Chinese clematis and Blackberrylily Rhizome mixing, add deionized water according to the mass ratio of 1:10-15, and heating continues little fiery boiling 1-2 hour after boiling, and is separated the dregs of a decoction and liquid after ceasing fire;
(5) by the cordate houttuynia filtrate of step (2), the distillate of step (3) and the liquid of step (4) mix and obtain the medicinal herb spray of the present invention for hospital's sterilizing.
Preferably for the preparation method of the medicinal herb spray of hospital's sterilizing, comprise the following steps:
(1) the Chinese medicine material difference grind into powder getting above-mentioned weight portion is for subsequent use;
(2) getting Heartleaf Houttuynia Herb, to join mass fraction according to the mass ratio of 1:5 be soak after 9 hours in the alcohol of 95%, and it is for subsequent use that cordate houttuynia filtrate is got in centrifugation;
(3) extracting honeysuckle, Fructus meliae toosendan, rattletop, it is add hot reflux in the alcohol of 90% 2.5 hours that the coptis and Ephedra mixing join mass fraction according to the mass ratio of 1:8, gets distillate for subsequent use;
(4) dogwood is got, cicada slough, rhizome of cyrtomium, mulberry leaf, the wind-weed, granatum, windproof, the Chinese pulsatilla, dandelion, kuh-seng, the root of Chinese clematis and Blackberrylily Rhizome mixing, add deionized water according to the mass ratio of 1:13, and heating continues little fiery boiling 1.5 hours after boiling, and is separated the dregs of a decoction and liquid after ceasing fire;
(5) by the cordate houttuynia filtrate of step (2), the distillate of step (3) and the liquid of step (4) mix and obtain the medicinal herb spray of the present invention for hospital's sterilizing.
Using method: spray filling for this product directly to eminence during use in sprayer, ventilation of can windowing after ten minutes.
The mechanism of action of each Chinese medicine:
Cordate houttuynia: pungent, be slightly cold, returns lung channel; There is clearing heat and detoxicating, the carbuncle that disappears apocenosis, inducing diuresis for removing edema, treating stranguria effect; Cure mainly the illness such as tonsillitis, lung abscess, urinary tract infections, dyspnea and cough due to lung-heat, malaria, oedema, carbuncle sore tumefacting virus, heat pouring, eczema, prolapse of the anus.
Dogwood: acid, tepor.Enter liver, kidney channel.Function cures mainly: tonify the liver and kidney, puckery smart arrest sweating.Control dizziness, dizzy, tinnitus, soreness and weakness of waist and knees, impotence and seminal emission lingering diarrhea, the enuresis, the incontinence of old man's frequent micturition, incessant sweating due to debility, menorrhalgia, vaginal dripping.
Cicada slough: sweet, cold.Return lung, Liver Channel.Function with cure mainly: dispelling wind and heat from the body, relieve sore throat, promoting eruption, removing nebula, spasmolysis.For anemopyretic cold, pharyngalgia hoarsen, measles without adequate eruption, rubella itch, cataract, convulsion, tetanus.
Chinese ephedra: warm in nature, pungent, the micro-hardship of taste.Function cures mainly: Pulvis Diaphoreticus is trembled with fear, and freeings lung and relieving asthma, inducing diuresis for removing edema.For anemofrigid cold, uncomfortable in chestly to breath with cough, geomantic omen edema, bronchial astehma.
Rhizome of cyrtomium: cold in nature, bitter; Slightly poisonous.Function cures mainly: clearing heat and detoxicating, hemostasis, desinsection.For cold, fever, dysentery, damp and hot, sore, has blood in stool, hematuria, menorrhalgia, and knife wound is hemorrhage, roundworm, rich worm, tapeworm disease, induced abortion, postpartum haemorrhage.
Fructus meliae toosendan: bitter, cold; Slightly poisonous.Return liver, small intestine, urinary bladder channel.Function with cure mainly: soothing the liverly to expel the heat-evil, promoting qi circulation and relieving pain, desinsection.For liver depression forming fire, the chest side of body, abdominal distention, hernia pain, abdominal pain due to enterositosis.
Honeysuckle: sweet, cold.Return lung, the heart, stomach warp.Function with cure mainly: clearing heat and detoxicating, dispelling wind and heat from the body.Treat sore, larynx numbness, erysipelas, toxic-heat and blood stasis, anemopyretic cold for carbuncle is swollen, warm disease is generated heat.
Mulberry leaf: cold in nature, taste is sweet, bitter.Function cures mainly: dispelling wind and heat from the body, and clearing away the lungheat and moisturizing is clear liver and improved vision.Dim-sighted for anemopyretic cold, lung-heat type cough, headache and dizzy, hot eyes.
The wind-weed: bitter, sweet, cold.Return lung, stomach, kidney channel.Function with cure mainly: clearing heat-fire, nourshing Yin and drynsessmoistening prescription.For fever caused by exogenous pathogenic factors, high hot polydipsia, lung-heat type cough, osteopyrexia and fever, interior heat is quenched one's thirst, the dry constipation of intestines.
Granatum: sour, puckery, temperature.Return large intestine channel.Function with cure mainly: relieving diarrhea with astringents, hemostasis, expelling parasite.For rushing down for a long time, protracted dysentery, has blood in stool, prolapse of the anus, uterine bleeding, under band, and abdominal pain due to enterositosis.
Windproof: warm in nature, taste is sweet, pungent.Function cures mainly: inducing diaphoresis to dispel wind, and victory is wet, only convulsion.For cold headache, arthralgia due to wind-dampness, spasm of limbs, rheumatism itch, tetanus.
The Chinese pulsatilla: bitter, cold.Return stomach, large intestine channel.Function with cure mainly: clearing heat and detoxicating, remove heat from the blood and relieve diarrhea.For toxic-heat and blood stasis, under pruritus vulvae band.
Dandelion: cold in nature, bitter, sweet.Function cures mainly: clearing heat and detoxicating, dispersing swelling and dissipating binds, inducing diuresis for treating strangurtia.Puckery pain is drenched for acute mastitis, scrofula, furuncle swelling toxin, pharyngalgia, lung carbuncle, acute appendicitis, hot eyes, jaundice with damp-heat pathogen, heat.
Kuh-seng: bitter, cold.The thoughts of returning home, liver, stomach, large intestine, urinary bladder channel.Function with cure mainly: heat-clearing and damp-drying drug, desinsection, diuresis.For hot dysentery, have blood in stool, jaundice renal shutdown, leukorrhea with reddish discharge, swelling of vulva pruritus vulvae, eczema, wet sore, pruitus, mange leprosy; Control trichomonas vaginitis outward.
Rattletop: pungent, micro-sweet, is slightly cold.Return lung, spleen, stomach, large intestine channel.Function with cure mainly: deliver promoting eruption, clearing heat and detoxicating, elevate a turnable ladder yang-energy.For headache due to pathogenic wind-heat, dentalgia, aphtha, abscess of throat, measles without adequate eruption, sun poison sends out spot, prolapse of the anus, the prolapse of uterus.
The coptis: bitter, cold.The thoughts of returning home, spleen, stomach, liver, courage, large intestine channel.Function with cure mainly: heat-clearing and damp-drying drug, purging intense heat and detonicating.Full for damp and hot ruffian, vomiting acid regurgitation, rush down dysentery, jaundice, unconsciousness due to high fever, heart-fire hyperactivity, dysphoria and insomnia, palpitation and uneasiness, blood-head tells nosebleed, hot eyes, and toothache, quenches one's thirst, and carbuncle is swollen treats sore; Control eczema outward, wet sore, duct is suppurated.
The root of Chinese clematis: pungent, salty, temperature.Return urinary bladder channel.Function with cure mainly: wines used as antirheumatic, stimulate the menstrual flow network.For arthralgia due to wind-dampness, extremity numbness, the contraction of muscle arteries and veins, joint stuffiness.
Blackberry lily: bitter, cold.Return lung channel.Function with cure mainly: clearing heat and detoxicating, dissolving phlegm, relieve sore throat.Tie for the malicious convulsive seizure due to phlegm-fire Tai of heat, abscess of throat, accumulation and obstruction of sputum, cough and asthma.
Compared with prior art the invention has the beneficial effects as follows:
Raw material of the present invention is natural Chinese medicinal herb, production cost is low, safety non-toxic is non-stimulated, staphylococcus aureus can be eliminated fast and effectively, Escherichia coli, staphylococcus albus, the various pathogens such as shigella dysenteriae, and to Leptospira, the multiple pathogenic microorganisms such as influenza virus and pathogenic moulds also has inhibitory action, there is fungicidal spectrum wide, sterilizing ability is strong, speed of action is fast, the advantages such as good stability, and this product ethanol content is low, non-explosive, nonflammable, use safety, have no irritating odor after using, human body reacts without discomfort, be applicable to all medical treatment, health, health institution, comprise situation of all-level hospitals, clinic, clinic, commune hospital, healthcare hospital for women & children, maternity hospital, sanatorium, health station, sanatorium, convalescent home, blood station, various clinics etc.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
The described medicinal herb spray for hospital's sterilizing, is made up of the Chinese medicine material of following weight portion:
Cordate houttuynia 12 parts, dogwood 16 parts, cicada slough 7 parts, 18 parts, Chinese ephedra, rhizome of cyrtomium 4 parts, Fructus meliae toosendan 15 parts, honeysuckle 20 parts, 5 parts, mulberry leaf, the wind-weed 3 parts, granatum 10 parts, windproof 10 parts, the Chinese pulsatilla 12 parts, dandelion 8 parts, kuh-seng 4 parts, rattletop 6 parts, the coptis 13 parts, 4 parts, the root of Chinese clematis, blackberry lily 2 parts.
The preparation method of the described medicinal herb spray for hospital's sterilizing, comprises the following steps:
(1) the Chinese medicine material difference grind into powder getting above-mentioned weight portion is for subsequent use;
(2) getting Heartleaf Houttuynia Herb, to join mass fraction according to the mass ratio of 1:5 be soak after 9 hours in the alcohol of 95%, and it is for subsequent use that cordate houttuynia filtrate is got in centrifugation;
(3) extracting honeysuckle, Fructus meliae toosendan, rattletop, it is add hot reflux in the alcohol of 90% 2.5 hours that the coptis and Ephedra mixing join mass fraction according to the mass ratio of 1:8, gets distillate for subsequent use;
(4) dogwood is got, cicada slough, rhizome of cyrtomium, mulberry leaf, the wind-weed, granatum, windproof, the Chinese pulsatilla, dandelion, kuh-seng, the root of Chinese clematis and Blackberrylily Rhizome mixing, add deionized water according to the mass ratio of 1:13, and heating continues little fiery boiling 1.5 hours after boiling, and is separated the dregs of a decoction and liquid after ceasing fire;
(5) by the cordate houttuynia filtrate of step (2), the distillate of step (3) and the liquid of step (4) mix and obtain the medicinal herb spray of the present invention for hospital's sterilizing.
Embodiment 2
The described medicinal herb spray for hospital's sterilizing, is made up of the Chinese medicine material of following weight portion:
Cordate houttuynia 10 parts, dogwood 15 parts, cicada slough 5 parts, 15 parts, Chinese ephedra, rhizome of cyrtomium 3 parts, Fructus meliae toosendan 10 parts, honeysuckle 15 parts, 3 parts, mulberry leaf, the wind-weed 2 parts, granatum 8 parts, windproof 8 parts, the Chinese pulsatilla 10 parts, dandelion 5 parts, kuh-seng 3 parts, rattletop 2 parts, the coptis 10 parts, 3 parts, the root of Chinese clematis, blackberry lily 1 part.
The preparation method of the described medicinal herb spray for hospital's sterilizing, comprises the following steps:
(1) the Chinese medicine material difference grind into powder getting above-mentioned weight portion is for subsequent use;
(2) getting Heartleaf Houttuynia Herb, to join mass fraction according to the mass ratio of 1:3 be soak after 8 hours in the alcohol of 95%, and it is for subsequent use that cordate houttuynia filtrate is got in centrifugation;
(3) extracting honeysuckle, Fructus meliae toosendan, rattletop, it is add hot reflux in the alcohol of 90% 2 hours that the coptis and Ephedra mixing join mass fraction according to the mass ratio of 1:5, gets distillate for subsequent use;
(4) dogwood is got, cicada slough, rhizome of cyrtomium, mulberry leaf, the wind-weed, granatum, windproof, the Chinese pulsatilla, dandelion, kuh-seng, the root of Chinese clematis and Blackberrylily Rhizome mixing, add deionized water according to the mass ratio of 1:10, and heating continues little fiery boiling 1 hour after boiling, and is separated the dregs of a decoction and liquid after ceasing fire;
(5) by the cordate houttuynia filtrate of step (2), the distillate of step (3) and the liquid of step (4) mix and obtain the medicinal herb spray of the present invention for hospital's sterilizing.
Embodiment 3
The described medicinal herb spray for hospital's sterilizing, is made up of the Chinese medicine material of following weight portion:
Cordate houttuynia 15 parts, dogwood 18 parts, cicada slough 8 parts, 20 parts, Chinese ephedra, rhizome of cyrtomium 5 parts, Fructus meliae toosendan 18 parts, honeysuckle 22 parts, 8 parts, mulberry leaf, the wind-weed 5 parts, granatum 12 parts, windproof 12 parts, the Chinese pulsatilla 15 parts, dandelion 10 parts, kuh-seng 6 parts, rattletop 10 parts, the coptis 15 parts, 5 parts, the root of Chinese clematis, blackberry lily 3 parts.
The preparation method of the described medicinal herb spray for hospital's sterilizing, comprises the following steps:
(1) the Chinese medicine material difference grind into powder getting above-mentioned weight portion is for subsequent use;
(2) getting Heartleaf Houttuynia Herb, to join mass fraction according to the mass ratio of 1:6 be soak after 10 hours in the alcohol of 95%, and it is for subsequent use that cordate houttuynia filtrate is got in centrifugation;
(3) extracting honeysuckle, Fructus meliae toosendan, rattletop, it is add hot reflux in the alcohol of 90% 3 hours that the coptis and Ephedra mixing join mass fraction according to the mass ratio of 1:10, gets distillate for subsequent use;
(4) dogwood is got, cicada slough, rhizome of cyrtomium, mulberry leaf, the wind-weed, granatum, windproof, the Chinese pulsatilla, dandelion, kuh-seng, the root of Chinese clematis and Blackberrylily Rhizome mixing, add deionized water according to the mass ratio of 1:15, and heating continues little fiery boiling 2 hours after boiling, and is separated the dregs of a decoction and liquid after ceasing fire;
(5) by the cordate houttuynia filtrate of step (2), the distillate of step (3) and the liquid of step (4) mix and obtain the medicinal herb spray of the present invention for hospital's sterilizing.
The medicinal herb spray measure of merit that the present invention is used for hospital's sterilizing is as follows:
Testing location: hospital registers the Room, waiting room, ward, corridor;
Method of testing: impingement method
Adopt MAS-100 air microorganism sampler, air 50L is gathered with 100L/min, make the aerial particle hits of carrying disease germs of suspension on nutrient agar panel, after then it being cultivated 48h in the insulating box of 36 ± 1 DEG C, calculate bacteria colony count contained in every cubic metres of air.
Medicinal herb spray of the present invention is added sprayer and carries out spraying disinfection to testing location, sprinkling consumption is 1-3mL/m 3.Respectively in use first 30 minutes of the present invention with use latter 30 minutes in the register Room, waiting room, ward, corridor of hospital and respectively get 2 samples apart from ground level about 1 meter place, calculate and sample bacterial clump mean value and sterilization rate, concrete data are as table 1:
Table 1
Cfu/m before sterilizing 3 Cfu/m after sterilizing 3 Sterilization rate %
To register No. 1, Room sample 568 36 93.66
To register No. 2, Room sample 571 42 92.64
Waiting room No. 3 samples 487 31 93.63
Waiting room No. 4 samples 514 29 94.36
No. 5, ward sample 325 27 91.69
No. 6, ward sample 331 23 93.05
No. 7, corridor sample 378 32 91.53
No. 8, corridor sample 403 29 92.80
Mean value 447.125 31.125 93.04
As can be seen from Table 1, sterilization rate of the present invention can reach more than 90%, and bactericidal effect is good, and have no irritating odor after using, human body reacts without discomfort.

Claims (3)

1., for a medicinal herb spray for hospital's sterilizing, it is characterized in that being made up of the Chinese medicine material of following weight portion:
Cordate houttuynia 10-15 part, dogwood 15-18 part, cicada slough 5-8 part, Chinese ephedra 15-20 part, rhizome of cyrtomium 3-5 part, Fructus meliae toosendan 10-18 part, honeysuckle 15-22 part, mulberry leaf 3-8 part, wind-weed 2-5 part, granatum 8-12 part, windproof 8-12 part, Chinese pulsatilla 10-15 part, dandelion 5-10 part, kuh-seng 3-6 part, rattletop 2-10 part, coptis 10-15 part, root of Chinese clematis 3-5 part, blackberry lily 1-3 part.
2. the medicinal herb spray for hospital's sterilizing according to claim 1, is characterized in that being made up of the Chinese medicine material of following weight portion:
Cordate houttuynia 12 parts, dogwood 16 parts, cicada slough 7 parts, 18 parts, Chinese ephedra, rhizome of cyrtomium 4 parts, Fructus meliae toosendan 15 parts, honeysuckle 20 parts, 5 parts, mulberry leaf, the wind-weed 3 parts, granatum 10 parts, windproof 10 parts, the Chinese pulsatilla 12 parts, dandelion 8 parts, kuh-seng 4 parts, rattletop 6 parts, the coptis 13 parts, 4 parts, the root of Chinese clematis, blackberry lily 2 parts.
3. a preparation method for the medicinal herb spray for hospital's sterilizing according to claim 1, is characterized in that comprising the following steps:
(1) the Chinese medicine material difference grind into powder getting above-mentioned weight portion is for subsequent use;
(2) get Heartleaf Houttuynia Herb according to the mass ratio of 1:3-6 join mass fraction be soak 8-10 hour in the alcohol of 95% after, it is for subsequent use that cordate houttuynia filtrate is got in centrifugation;
(3) extracting honeysuckle, Fructus meliae toosendan, rattletop, it is add hot reflux 2-3 hour in the alcohol of 90% that the coptis and Ephedra mixing join mass fraction according to the mass ratio of 1:5-10, gets distillate for subsequent use;
(4) dogwood is got, cicada slough, rhizome of cyrtomium, mulberry leaf, the wind-weed, granatum, windproof, the Chinese pulsatilla, dandelion, kuh-seng, the root of Chinese clematis and Blackberrylily Rhizome mixing, add deionized water according to the mass ratio of 1:10-15, and heating continues little fiery boiling 1-2 hour after boiling, and is separated the dregs of a decoction and liquid after ceasing fire;
(5) by the cordate houttuynia filtrate of step (2), the distillate of step (3) and the liquid of step (4) mix and obtain the medicinal herb spray of the present invention for hospital's sterilizing.
CN201510828680.0A 2015-11-24 2015-11-24 Traditional Chinese medicine spraying agent for hospital sterilization and disinfection and preparation method thereof Pending CN105360183A (en)

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CN108576081A (en) * 2018-01-24 2018-09-28 合肥华盖生物科技有限公司 A kind of medical equipment environment-friendly type Chinese medicine antiseptic and preparation method thereof
CN108371192A (en) * 2018-02-23 2018-08-07 荆丽杰 A kind of Traditional Chinese medicine disinfectant and preparation method thereof for medical waste coagulation
CN110278968A (en) * 2019-05-13 2019-09-27 李汝新 A kind of manufacturing method of clinical care disinfectant

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Application publication date: 20160302