CN105359909B - A method of for magnesium deficiency induction Niu Heer navel orange veins explosion - Google Patents
A method of for magnesium deficiency induction Niu Heer navel orange veins explosion Download PDFInfo
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- CN105359909B CN105359909B CN201510681686.XA CN201510681686A CN105359909B CN 105359909 B CN105359909 B CN 105359909B CN 201510681686 A CN201510681686 A CN 201510681686A CN 105359909 B CN105359909 B CN 105359909B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The present invention provides a kind of methods for magnesium deficiency induction Niu Heer navel orange veins explosion, include the following steps：（a）Annual spring chooses 2 years raw trifoliate orange anvil Niu Heer navel orange graftings, pruned, stem, supply deionized water carries out sand culture field planting, hardening after leaf；（b）With 1/4 improvement Hoagland and Aron formula nutritional liquid culture graftings（c）Magnesium deficiency coercing cultivation is carried out to grafting with improvement Hoagland and Aron formula nutritionals liquid；（d）Young sprout growth is controlled by trimming means, promotes the explosion of 2 age old leaf veins, and disclose each nutrient solution prescription.The present invention makes biennial Niu Erheer navel oranges plant 2 age old leaf be in serious magnesium deficiency level in a short time, and significant, typical vein Decrepitation Phenomena is occurring for the year, and easy to operate, is easy to grasp.
Description
Technical field
The present invention relates to a kind of methods for magnesium deficiency induction Niu Heer navel orange veins explosion.
Background technology
China has become the world's largest Niu Heer navel oranges place of production, and south jiangxi is the Niu Heer navel oranges producing region of Largest In China.Closely
There is the blade nutritional deficiency yellow of large area and vein in succession with increase south jiangxi numerous Niu Heer navel oranges garden of the result time limit in Nian Lai
Swollen to split phenomenon, the few Red Soils In A Hill Region of organic fertilizer application and sandy soil orchard are particularly acute.In general, swollen split of vein is identified citrus
The classical symptom of boron deficiency, therefore the use always of the upper Newhall navel orange of production is applied boron and is rescued.But it is rectified through long-term boron of applying
It controls, the swollen symptom split of south jiangxi producing region Niu Heer navel oranges yellowing leaf, vein still fails to eliminate.Since 2008, inventor is to Jiangxi
Southern navel orange producing region vein is swollen to split and (is accompanied by yellowing leaf) that symptom orchard is investigated, and analyzes the full nutritive element of blade
Situation shows that most of orchard blade boron contents have been in appropriate and a large amount is horizontal, but generally existing magnesium lacks or deficiency,
Tentatively judge this symptom for caused by magnesium deficiency.Then, Li Jian etc. in the field investigation of Yongan City, Fujian Province it has also been found that magnesium deficiency can lead to knob lotus
Your navel orange vein is swollen to be split；2009-2011, inventor apply magnesium experiment in the field of south jiangxi and find that vein can effectively be rescued by applying magnesium
It is swollen to split；Yellow mirror in 2011 is great to be waited using Youxi gold mandarin orange, the annual aging Spring leaf of Niu Heer navel oranges and Guanxi small stream honey shaddocks orchard as material
Material, have studied blade vascular tissue structure change under the conditions of magnesium deficiency, propose xylem obstruction degenerate with irregular hyperplasia and
The degeneration of marrow cell is that magnesium deficiency typical organization is abnormal.Further demonstrating magnesium deficiency stress can cause Niu Heer navel orange veins swollen
It splits.
But the swollen research for splitting mechanism of magnesium deficiency Niu Heer navel oranges vein at present is seldom, is concentrated mainly on field inspection.
This is not only the reason inadequate to its attention degree early period, also with there is no under magnesium deficiency stress conditions induce Niu Heer navel orange veins it is quick-fried
The method split is related.The foundation of correlation technique mainly faces the problem of following aspect：First, Niu Heer navel orange are grafting, water
Culture method is it is difficult to ensure that its long-term normal survival；Second, whether long-term existence sand culture mode ensures that citrus magnesium deficiency is disputed on；The
Three, it there is no the magnesium deficiency culture formula of liquid that can ensure that Niu Heer navel orange leaf nutrition element balances at present；4th, since magnesium is plant
The moderate-element of body, the ratio in citrus leaves are higher than other positions, so being lacked it is difficult to which blade is made to reach serious in a short time
Mistake degree.Since 2011, inventor, which gradually starts to explore, established relevant abductive approach, has grasped coerced in magnesium deficiency at present
The method of vein explosion occurs for induction Niu Heer navel oranges current year under the conditions of compeling.
Invention content
The purpose of the present invention is to provide a kind of short magnesium deficiencies that is used for easy to operate, time-consuming to induce Niu Heer navel orange veins quick-fried
The method split.
The purpose of the present invention is what is realized by following measures：
A method of for magnesium deficiency induction Niu Heer navel orange veins explosion, include the following steps：
(a) annual spring chooses 2 years raw trifoliate orange anvil Niu Heer navel orange graftings, pruned, stem, supply deionized water after leaf
Carry out sand culture field planting, hardening；
(b) with 1/4 improvement Hoagland and Aron formula nutritional liquid culture graftings；
(c) magnesium deficiency coercing cultivation is carried out to grafting with improvement Hoagland and Aron formula nutritionals liquid；
(d) young sprout growth is controlled by trimming means, promotes the explosion of 2 age old leaf veins；
In the step b), 1/4 improvement Hoagland and Aron formula nutritional formula of liquid is：Ca(NO3)2·4H2O
0.8125mmol·L-1, KNO3 0.1575mmol·L-1, NH4NO3 0.125mmol·L-1, KH2PO4 0.125mmol·L-1,
MgSO4·7H2O 0.375mmol·L-1, FeSO4-EDTA 6.25μmol·L-1, H3BO3 3.45μmol·L-1, MnSO4·
4H2O3μmol·L-1, ZnSO4·7H2O 0.95μmol·L-1, CuSO4·5H2O 0.175μmol·L-1, Na2MoO4 0.5μ
mol·L-1, pH 6.0；
In the step c), improvement Hoagland and Aron formula nutritional formula of liquid is：Ca(NO3)2·4H2O
3.25mmol·L-1, KNO3 0.63mmol·L-1, NH4NO3 0.5mmol·L-1, KH2PO4 0.5mmol·L-1, Na2SO4
1.5mmol·L-1, FeSO4-EDTA 25μmol·L-1, H3BO3 13.8μmol·L-1, MnSO4·4H2O 12μmol·L-1,
ZnSO4·7H2O 3.8μmol·L-1, CuSO4·5H2O 0.7μmol·L-1, Na2MoO4 2μmol·L-1, pH 6.0.
According to embodiments of the present invention, the present invention can also have following additional technical characteristic：
Above-mentioned steps a) includes：A-1 diameter of stock about 1.5cm, the height well-grown graftings of about 35cm) are chosen, it will
Plant above ground portion is cut to 20cm or so, there are 52 age old leaf (last year autumn growth blade), main root is cut to 5cm or so；A-2 it) is colonized
Carry out potting sand culture (quartz sand: perlite volume ratio is 1: 1), supply deionized water hardening 10-20d.
The hardening time is preferably the annual 3-4 months in above-mentioned steps a).
In above-mentioned steps b), 1/4 improvement Hoagland and Aron formula nutritional liquid incubation times are preferably 30d.
Above-mentioned steps d) includes：D-1 the spring tip control) sprouted is 3 branches, spring leaf toss about in bed entirely it is green after trimming to each branch
Retain 3 spring leaves；D-2) sprout the summer and autumn tip open up entirely after young leaves, turn it is green after by trimming removal.
Advantageous effect
1. the present invention keeps biennial Niu Erheer navel oranges plant 2 age old leaf horizontal in serious magnesium deficiency in a short time, and
Significant, typical vein Decrepitation Phenomena occurs for current year, and easy to operate, is easy to grasp.
2. Niu Heer navel oranges are grafting, the present invention ensures citrus magnesium deficiency using sand culture mode；And ensure to make leaf in a short time
Piece reaches serious magnesium missing, and induces Niu Heer navel orange current years that the classical symptom that vein bursts occurs, such as the green area of leaf base
Domain is in typical " ∧ " font.
3. of the invention ensureing that so that blade is reached serious magnesium missing in a short time induces Niu Heer navel orange current year generation veins quick-fried
In the case of splitting, other elements, such as trace element, moderate-element and a great number of elements keep normal level.
Description of the drawings
Flow chart of Fig. 1 present invention for the method for magnesium deficiency induction Niu Heer navel orange veins explosion；
The design sketch of Fig. 2 embodiments 1；
The design sketch of the magnesium deficiency induction Niu Heer navel orange veins explosion of 2 magnesium deficiency group of Fig. 3 embodiments；
The blade master pulse of 2 magnesium deficiency group of Fig. 4 embodiments and lateral vein changes in microstructure figure.
Specific implementation mode
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings.By reference to attached drawing
The embodiment of description is exemplary, and is only used for explaining the present invention, and is not considered as limiting the invention.
The side according to the ... of the embodiment of the present invention for magnesium deficiency induction Niu Heer navel orange veins explosion is described with reference to Fig. 1
Method.
As shown in Figure 1, the method according to the ... of the embodiment of the present invention for magnesium deficiency induction Niu Heer navel orange veins explosion is specific
Include the following steps：
(a) annual spring chooses 2 years raw trifoliate orange anvil Niu Heer navel orange graftings, pruned, stem, supply deionized water after leaf
Carry out sand culture field planting, hardening；
(b) with 1/4 improvement Hoagland and Aron formula nutritional liquid culture graftings；
(c) magnesium deficiency coercing cultivation is carried out to grafting with improvement Hoagland and Aron formula nutritionals liquid；
(d) young sprout growth is controlled by trimming means, promotes the explosion of 2 age old leaf veins.
Method according to the ... of the embodiment of the present invention for magnesium deficiency induction Niu Heer navel orange veins explosion as a result, can make two
It is horizontal and quick-fried significant vein occurs for the year that year Sheng Niuerheer navel oranges plant 2 age old leaf is in serious magnesium deficiency in a short time
Phenomenon is split, and easy to operate, is easy to grasp.
In step (a), in order to meet needed for quantification experimental study, raw trifoliate orange anvil Niu Heer navel orange graftings need within 2 years
Unified trimming is carried out, and passes through field planting, the sample strain that hardening is grown and nutritional condition is almost the same.Preferably, according to this
One embodiment of invention, specific processing method may include：
A-1 diameter of stock about 1.5cm, the height well-grown graftings of about 35cm) are chosen, by plant above ground portion cut to
20cm or so, there are 52 age old leaf (last year autumn growth blade), main root is cut to 5cm or so；
A-2) field planting carries out potting sand culture (quartz sand: perlite volume ratio is 1: 1), supply deionized water hardening 10-
20d。
Trimming, field planting and the hardening of Niu Heer navel orange graftings can carry out in annual spring.Reality according to the present invention
Apply example, the preferably annual 3-4 months.
In step (b), according to an embodiment of the invention, 1/4 improvement Hoagland and Aron formula nutritional formula of liquid,
Formula is：Ca(NO3)2·4H2O 0.8125mmol·L-1, KNO3 0.1575mmol·L-1, NH4NO3 0.125mmol·L-1,
KH2PO4 0.125mmol·L-1, MgSO4·7H2O 0.375mmol·L-1, FeSO4-EDTA 6.25μmol·L-1,
H3BO33.45μmol·L-1, MnSO4·4H2O 3μmol·L-1, ZnSO4·7H2O 0.95μmol·L-1, CuSO4·5H2O
0.175μmol·L-1, Na2MoO4 0.5μmol·L-1, pH 6.0.
According to one embodiment of present invention, incubation time is preferably 30d.Due to step (b) primarily to promoting to plant
Plant shape is at sand culture root system, it is ensured that plant root nutrient absorption ability is normal, consistent, so the incubation time of step (b) not only limits
In the variation of same purpose.
In step (c), Niu Heer navel oranges plant grows under complete magnesium deficiency stress conditions, develops.It is according to the present invention
One embodiment, using following improvement Hoagland and Aron formula nutritional formula of liquid：Ca(NO3)2·4H2O 3.25mmol·
L-1, KNO3 0.63mmol·L-1, NH4NO3 0.5mmol·L-1, KH2PO4 0.5mmol·L-1, NaSO4 1.5mmol·L-1,
FeSO4-EDTA 25μmol·L-1, H3BO3 13.8μmol·L-1, MnSO4·4H2O 12μmol·L-1, ZnSO4·7H2O
3.8μmol·L-1, CuSO4·5H2O 0.7μmol·L-1, Na2MoO4 2μmol·L-1, pH 6.0.
In step (d), the magnesium deficiency stress level that appropriate time trimming young sprout may advantageously facilitate 2 age old leaf is held.According to
The embodiment of the present invention, preferably：Sprouting the spring tip control be 3 branches, spring leaf toss about in bed entirely it is green after trimming to each branch retain 3
A spring leaf；The summer and autumn tip of sprouting opens up entirely after young leaves, turn green after removed by trimming.
1 magnesium deficiency of embodiment induces Niu Heer navel orange veins explosion Initial Experimental Stage
Diameter about 0.5cm, the height well-grown anvil Niu Heer navel orange graftings of about 30cm are chosen, plant above ground portion is cut
To 20cm high or so, there are 5 autumn growth blades in 2010, main root is cut to 5cm or so.On March 23rd, 2011, field planting carried out potting
Sand culture test adds quartz sand and perlite (volume ratio 1: 1) 8kg, pallet is put at basin bottom per one plant of basin.Early period only supplies deionization
Water carries out hardening.
On April 15th, 2011 carries out magnesium deficiency coercing cultivation.Culture solution uses Hoagland (1/2 concentration) and the full concentration of Aron
Formula nutritional liquid：
Normally group of formula is：Ca(NO3)2·4H2O 2mmol·L-1, KNO3 3mmol·L-1, NH4H2PO4 0.5mmol·
L-1, MgSO4·7H2O 1mmol·L-1, H3BO3 46μmol·L-1, MnCl2·4H2O 6μmol·L-1, ZnSO4·7H2O
0.7μmol·L-1, CuSO4·5H2O 0.3μmol·L-1, H2MoO4 1μmol·L-1, 25 μm of olL of Fe-EDTA-1, pH
6.0；
Magnesium deficiency group of formula is：Ca(NO3)2·4H2O 2mmol·L-1, KNO3 3mmol·L-1, NH4H2PO4 0.5mmol·
L-1, Na2SO4 1mmol·L-1, H3BO3 46μmol·L-1, MnCl2·4H2O 6μmol·L-1, ZnSO4·7H2O 0.7μ
mol·L-1, CuSO4·5H2O 0.3μmol·L-1, H2MoO4 1μmol·L-1, 25 μm of olL of Fe-EDTA-1, pH 6.0.
During magnesium deficiency Stress treatment, per each basin teeming 500ml nutrient solutions of 1-2d, salt is washed with deionized water 1 time per 7-10d,
Prevent salt accumulation.
Magnesium deficiency vein is swollen to split Symptom Observation：As shown in Fig. 2, when experiment process 4 months, magnesium deficiency 2 age old leaf yellow, on blade tip
Portion and leaf base master pulse be not nearby in "∧" type green ("∧" type green is true to type simultaneously), and explosion symptom occurs in lateral vein.
11 kinds of constituent content analysis such as 4 spring at monthly age tip leaf nitrogen, phosphorus, potassium：Citrus plant nutrient status is generally by the 4-6 spring at monthly age
The nutritive element content of tip leaf judges.Therefore, in order to verify the nutrition condition and sand culture of sand culture trifoliate orange anvil Niu Heer navel oranges
Concentration formula, when experiment process 4 months, take 1 spring in age tip leaf to measure 11 kinds of nutritive element contents such as nitrogen, phosphorus, potassium.Wherein Leaf N
After Sulfuric-Acid Digestion, it is measured with hydrochloric titration.Phosphorus is nitric acid-perchloric acid resolution, and molybdenum antimony resistance colorimetric method is measured.
Potassium, calcium, magnesium, iron, manganese, zinc, copper are nitric acid-perchloric acid resolution, and (PerkinElmer is public for AA-800 atomic absorption spectrophotometers
Department) it is measured.Boron is dry ashing method, and (the general analysis all purpose instrument Limited Liability in Beijing is public for TU-1901 ultraviolet-uisible spectrophotometers
Department) it is measured under 430nm wavelength.As shown in table 1, normally group leaf magnesium is slightly below optimum range, and magnesium deficiency group leaf magnesium is in and lacks
Range, phosphorus, sulphur and iron are in optimum range, and boron a large amount, potassium is excessive, and nitrogen, calcium, manganese, zinc and copper are less than optimum range.
The magnesium deficiency sand culture hydroponics Niu Heer navel oranges of the embodiment can induce the swollen appearance for splitting feature of its vein, but logical
Foliar nutrient diagnosis is crossed, there is magnesium missings for tree body, and boron potassium is in a large amount level above, and nitrogen, calcium, manganese, zinc and copper are less than suitable
Suitable range, being indicated above swollen split of Niu Heer navel orange vein atypia may be related with magnesium shortage, the excessive synergistic effect of boron potassium, and
Non- simple magnesium deficiency induction Niu Heer navel orange veins are swollen to be split.
Niu Heer navel orange 1 age Spring leaf nutritive element contents when 1 experiment process April of table
Note：Leaf nutrition element optimum range is drafted with reference to Peng Liangzhi etc.《South jiangxi navel orange Foliar nutrient diagnosis technical regulation》
(DB 36/T 625-2011)
2 simple magnesium deficiency of embodiment induces Niu Heer navel orange vein burst experiments
As shown in Figure 1, choosing diameter of stock about 1.5cm, height well-grown 2 years raw trifoliate orange anvil Niu Heer navel oranges of about 35cm
Grafting, plant above ground portion is cut to 20cm or so, there are 52 age old leaf (autumn growth blade in 2013), main root is cut to the left sides 5cm
It is right.On March 18th, 2014, field planting carried out potting sand culture test, per one plant of basin, added quartz sand and perlite (volume ratio 1: 1) 8kg,
Pallet is put at basin bottom, and supply deionized water carries out hardening 15d.
Hoagland the and Aron formula nutritional liquid culture graftings 30d of improvement 3 days 1/4 April in 2014.Its formula is：
Ca(NO3)2·4H2O 0.8125mmol·L-1, KNO3 0.1575mmol·L-1, NH4NO3 0.125mmol·L-1,
KH2PO40.125mmol·L-1, MgSO4·7H2O 0.375mmol·L-1, FeSO4-EDTA 6.25μmol·L-1, H3BO3
3.45μmol·L-1, MnSO4·4H2O 3μmol·L-1, ZnSO4·7H2O 0.95μmol·L-1, CuSO4·5H2O 0.175μ
mol·L-1, Na2MoO4 0.5μmol·L-1, pH 6.0.
May 3 carried out magnesium deficiency coercing cultivation.Culture solution is using Hoagland the and Aron formula nutritional liquid improved：Normally
Group of formula is：Ca(NO3)2·4H2O 3.25mmol·L-1, KNO3 0.63mmol·L-1, NH4NO3 0.5mmol·L-1,
KH2PO4 0.5mmol·L-1, MgSO4·7H2O 1.5mmol·L-1, FeSO4-EDTA 25μmol·L-1, H3BO313.8μ
mol·L-1, MnSO4·4H2O 12μmol·L-1, ZnSO4·7H2O 3.8μmol·L-1, CuSO4·5H2O 0.7μmol·L-1, Na2MoO4 2μmol·L-1, pH 6.0.Magnesium deficiency group of formula：Ca(NO3)2·4H2O 3.25mmol·L-1, KNO3
0.63mmol·L-1, NH4NO3 0.5mmol·L-1, KH2PO4 0.5mmol·L-1, Na2SO4 1.5mmol·L-1, FeSO4-
EDTA 25μmol·L-1, H3BO3 13.8μmol·L-1, MnSO4·4H2O 12μmol·L-1, ZnSO4·7H2O 3.8μ
mol·L-1, CuSO4·5H2O 0.7μmol·L-1, Na2MoO4 2μmol·L-1, pH 6.0.During magnesium deficiency Stress treatment, often
Its nutrient solution 1 time, it is ensured that there are the solution of 2cm or so depths in cultivation tray bottom tray；Salt is washed with deionized water 1 time per 10d,
Prevent salt accumulation.
Meanwhile during magnesium deficiency Stress treatment, the control of the spring tip of sprouting is 3 branches, spring leaf toss about in bed entirely it is green after trimming to each
Branch retains 3 spring leaves, and plant nutrient status is detected with spring leaf；Viviparous summer shoot and autumn growth are planted in trimming, and the trimming time is
Young leaves is opened up, turns green young sprout entirely, until plant 2 age old leaf vein bursts.
2 age old leaf magnesium element contents measure：Magnesium deficiency takes 2 age old leaf to measure magnesium element content, 3 repetitions during coercing.Leaf
Piece measures magnesium elements after nitric acid-perchloric acid resolution using AA-800 atomic absorption spectrophotometers (PerkinElmer companies)
Content.
The magnesium element content of Niu Heer navel oranges 2 age old leaf during 2 magnesium deficiency of table is coerced
As shown in Table 2, with the magnesium element content of increase embodiment 2 Niu Heer navel oranges, the 2 age old leaf of magnesium deficiency stress time
Trend, and substantially less than normal level (2.5~5.0mg/g) are reduced in significant.
11 kinds of constituent content analysis such as 4 spring at monthly age tip leaf nitrogen, phosphorus, potassium：1 spring in age tip leaf is taken to measure when experiment process 4 months
11 kinds of constituent contents such as nitrogen, phosphorus, potassium.As shown in Table 3, normal processing group all elements are in optimum range in example 2,
And magnesium deficiency group is less than optimum range except magnesium elements, other elements are in optimum range.
Niu Heer 4 monthly age of navel orange Spring leaf nutritive element contents when 3 experiment process April of table
Note：Leaf nutrition element optimum range is drafted with reference to Peng Liangzhi etc.《South jiangxi navel orange Foliar nutrient diagnosis technical regulation》
(DB 36/T 625-2011)
Magnesium deficiency vein is swollen to split Symptom Observation：As shown in figure 3, when experiment process 6 months, magnesium deficiency 2 age old leaf leaf base it is green
Color region is in typical " ∧ " font, and explosion symptom occurs in lateral vein.
The microscopic change of embodiment 4 blade master pulse and lateral vein institutional framework
Microstructure of leaf is observed：Using 2 age autumn growth old leaf of embodiment 2 as object, using traditional paraffin section preparation methods.It is main
Arteries and veins slice thickness is 6 μm, and lateral vein slice thickness is 5 μm；And using sarranine, it is fast green to dye, resinene mounting, observation master pulse and
The fibre structure of lateral vein changes.Picture is obtained by OLMPUS BX-51 micro imaging systems.
Test result is as shown in Figure 4.Wherein, A：2nd month master pulse；B：4th month master pulse；C：Primary phleom cell moves back
Change (red arrow)；D：6th month master pulse, phloem cell significantly grow increase (black arrow)；E：Phloem cell increases (black arrow
Head), cytoclasis (red arrow)；F：Master pulse bursts；G：2nd month lateral vein；H：4th month lateral vein, phloem cell hyperplasia are (red
Arrow)；I：Phloem cell hyperplasia (red arrow)；J：6th month phloem cell significantly increases (red arrow)；K：6th month
Lateral vein, bast disintegrate (red arrow), and tracheid disintegrates (black arrow)；L：Lateral vein bursts.1：Marrow；2：Xylem；3：
Bast；4：Fibrous sheath；5：Cortex.
As shown in Figure 4, magnesium deficiency stress causes phloem cell quantity significantly to increase (Fig. 4, A-D), processing group at the 4th month
Primary phloem tissue is squeezed and irregular fold occurs, and part primary phleom parenchyma cell is extruded broken (Fig. 4, C)；
Processing group phloem cell raised growth, increase at 6th month, part metaphloem cell, which is squeezed, to be crushed pure (Fig. 4, D, E)；
The xylem structure of individual blade tip positions master pulse gets muddled, and wood radiaftive rays is imperfect, and xylem parenchyma cell is dissipated with vessel element
Disorderly distribution, abaxial side part parenchyma cell are extruded broken death, and vein bursts (Fig. 4, F).
It is also known by Fig. 4, with the notable hyperplasia of extension phloem cell of test period.It is similar to master pulse, magnesium deficiency stress
Processing group phloem cell quantity significantly increases (Fig. 4, G-I) at 4th month, wooden when especially magnesium deficiency was coerced to 6th month
Interface between portion and bast disappears, and phloem cell significantly grows, increases (Fig. 4, J), and extruding leads to xylem structure more
It dissipates, wood radiaftive rays is imperfect (Fig. 4, K), and abaxial side part parenchyma cell is extruded broken, lateral vein explosion (Fig. 4, L).
The method for magnesium deficiency induction Niu Heer navel orange veins explosion described according to embodiments of the present invention 2, can make two
It is horizontal and quick-fried significant vein occurs for the year that year Sheng Niuerheer navel oranges plant 2 age old leaf is in serious magnesium deficiency in a short time
Phenomenon is split, and induces Niu Heer navel orange veins are swollen to split for simple magnesium deficiency.And it is easy to operate, it is easy to grasp.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not
In the case of being detached from the principle of the present invention and objective a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The range of invention is limited by claim and its equivalent.
Claims (4)
1. a kind of method for magnesium deficiency induction Niu Heer navel orange veins explosion includes the following steps：
（a）Annual spring chooses 2 years raw trifoliate orange anvil Niu Heer navel orange graftings, pruned, stem, supply deionized water carries out after leaf
Sand culture field planting, hardening；
（b）With 1/4 improvement Hoagland and Aron formula nutritional liquid culture graftings；
（c）Magnesium deficiency coercing cultivation is carried out to grafting with improvement Hoagland and Aron formula nutritionals liquid；
（d）Young sprout growth is controlled by trimming means, promotes the explosion of 2 age old leaf veins;The step（b）In, 1/4 improvement
Hoagland and Aron formula nutritional formula of liquid is：Ca(NO3)2·4H2O 0.8125 mmol·L-1, KNO3 0.1575
mmol·L-1, NH4NO3 0.125 mmol·L-1, KH2PO4 0.125 mmol·L-1, MgSO4·7H2O 0.375 mmol·
L-1, FeSO4- EDTA 6.25 µmol·L-1, H3BO3 3.45 µmol·L-1, MnSO4·4H2O 3 µmol·L-1,
ZnSO4·7H2O 0.95 µmol·L-1, CuSO4·5H2O 0.175 µmol·L-1, Na2MoO4 0.5 µmol·L-1, pH
6.0；
The step（c）In, improvement Hoagland and Aron formula nutritional formula of liquid is：Ca(NO3)2·4H2O 3.25
mmol·L-1, KNO3 0.63 mmol·L-1, NH4NO3 0.5 mmol·L-1, KH2PO4 0.5 mmol·L-1, Na2SO4 1.5
mmol·L-1, FeSO4-EDTA 25 µmol·L-1, H3BO3 13.8µmol·L-1, MnSO4·4H2O 12 µmol·L-1,
ZnSO4·7H2O 3.8 µmol·L-1, CuSO4·5H2O 0.7 µmol·L-1, Na2MoO4 2 µmol·L-1, pH 6.0；
Step (a）Including：（a-1）Diameter of stock about 1.5cm, the height well-grown graftings of about 35cm are chosen, will be planted
Strain overground part is cut to 20cm or so, there are 52 age old leaf, main root is cut to 5cm or so；
(a-2）Field planting carries out potting sand culture, and Shi Ying sand ﹕ perlite volume ratios are 1 ﹕ 1, supply deionized water hardening 10-20d.
2. the method for magnesium deficiency induction Niu Heer navel orange veins explosion as described in claim 1, the step（a）Middle hardening
Time is the annual 3-4 months.
3. the method for magnesium deficiency induction Niu Heer navel orange veins explosion as claimed in claim 1 or 2,1/4 improvement
Hoagland and Aron formula nutritional liquid incubation times are 30d.
4. the method for magnesium deficiency induction Niu Heer navel orange veins explosion as claimed in claim 1 or 2, the step（d）Packet
It includes:(d-1) sprout the spring tip control be 3 branches, spring leaf toss about in bed entirely it is green after trimming to each branch retain 3 spring leaves；(d-2)
The summer and autumn tip of sprouting opens up entirely after young leaves, turn green after removed by trimming.
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