CN105359826A - Technology of inducing hypsizygusmarmoreus budding by using chitosan oligosaccharide - Google Patents
Technology of inducing hypsizygusmarmoreus budding by using chitosan oligosaccharide Download PDFInfo
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- CN105359826A CN105359826A CN201510753828.9A CN201510753828A CN105359826A CN 105359826 A CN105359826 A CN 105359826A CN 201510753828 A CN201510753828 A CN 201510753828A CN 105359826 A CN105359826 A CN 105359826A
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- bacterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a technology of inducing hypsizygusmarmoreus budding by using chitosan oligosaccharide, which comprises a procedure ofpreparinga medium; a procedure of putting the medium into a fungus bottle/fungus sack; a procedure of disinfection; a procedure of inoculation and spawn running; a procedure of fungi scratching and budding inducing. The procedure of fungi scratching and budding inducing comprises the steps of scratching the periphery of the medium after inoculation and spawn running to form a circular groove; injecting a Chitosan oligosaccharide solution with a concentration of 0.03-0.07g/ml into the fungus bottle/fungus sack; removing the effusion of the Chitosan oligosaccharide solution inside the circular groove after the medium absorbsthe solutionfully for 0.8-1.2h to start the budding inducing process; for budding inducing, placing the fungus bottle/fungus sack in a room and covering the fungus bottle/fungus sack with perforated plastic film or non-woven fabric, wherein the indoor temperature is 14-16 DEG C, the indoor humidity is higher than 90%, the indoor carbon dioxide concentration is0.1-0.2%, the illuminance at the initial stage of mushroom budding is 50-100 lux, and the illuminance at the middle and late stage of the mushroom budding is 500-1000 lux. According to the invention, the asexual growth period of hypsizygusmarmoreus can be shortened; buds grow earlier; and the output of hypsizygusmarmoreus can be raised in a certain degree.
Description
Technical field
The invention belongs to seafood mushroom planting technology field, especially a kind of chitosan oligosaccharide induction seafood mushroom is buddingged technology.
Background technology
Seafood mushroom has very high edible and medical value, and its bacterial context is plump, delicate mouthfeel, fragrant, delicious flavour, normal food seafood mushroom has anticancer, anti-cancer, improves immunity, effect anti-aging, life-extending in advance, and it has become urban and rural residents and has consumed popular kind.But the existing production technology of seafood mushroom is still perfect not, and the growth cycle of seafood mushroom is longer, and particularly the mycelium stimulation of seafood mushroom urges flower bud operation, its quality directly affects seafood mushroom growth cycle and quality.Therefore, research and development are a kind of accelerates seafood mushroom mycelium physiological ripening, and the technology of inducing it to budding fast is very important.
Chitosan oligosaccharide has active cell, the formation of promotion plant callus, regulating growth of plants, adjustment Nutrient Absorption, the fertility that improves, diseases prevention, disease-resistant and effect such as raising output, oil recovery enhancement etc.Research finds, chitosan oligosaccharide had certain effect to production cycles such as shortening Xingbao mushroom, woodear, flat mushrooms.
Summary of the invention
The present invention is intended to overcome above-mentioned the deficiencies in the prior art, and provide the technology of buddingging of a kind of chitosan oligosaccharide induction seafood mushroom, this technology can shorten the asexual growth cycle of seafood mushroom, buddings ahead of time, and improves the output of seafood mushroom to a certain extent.
The present invention is achieved through the following technical solutions above-mentioned purpose:
A kind of chitosan oligosaccharide induction seafood mushroom is buddingged technology, comprises the following steps:
(1) medium preparation process, the batching of medium is cotton seed hulls 35 ~ 45%, wood chip 34 ~ 38%, wheat bran 15 ~ 20%, corn flour 1.5 ~ 3%, carbonic acid fine particle calcium carbonate 0.8 ~ 1.2%, lime 0.8 ~ 1.2%, pH value 7.5 ~ 8, water content 60 ~ 70%;
(2) medium loads bacterium bottle/bacterium bag process, is loaded by medium in the bacterium bottle/bacterium bag of 700 ~ 900ml, and every bottle/every bag culture medium siccative is 240 ~ 260g;
(3) sterilization process, first needs the cold air in pot all to get rid of, then under bacterium bottle/bacterium bag is remained on the temperature of 121 ~ 128 DEG C, maintains 2 ~ 2.5h, then make bacterium bottle/bacterium bag be cooled to less than 30 DEG C;
(4) bacterium operation is sent out in inoculation, aseptically carries out strain inoculation to the medium in bacterium bottle/bacterium bag, after strain inoculation completes, indoor temperature is controlled within the scope of 22 ~ 25 DEG C, by CO
2concentration remains on the bacterium process that less than 0.4% carries out seafood mushroom;
(5) mycelium stimulation urges flower bud operation, its processing method is, the surrounding of the medium sent out after bacterium operation through described inoculation is scratched except forming circular groove, in bacterium bottle/bacterium bag, implantation concentration is the chitosan oligosaccharide solution of 0.03 ~ 0.07g/ml, and medium fully absorbs through 0.8h ~ 1.2h and enters the process of urging flower bud by after the chitosan oligosaccharide solution hydrops removal in circular groove;
The described method of flower bud of urging is, bacterium bottle/bacterium bag is inserted indoor and is hidden by bacterium bottle/bacterium bag with porose plastic film or non-woven fabrics, the temperature of described indoor is 14 ~ 16 DEG C, and humidity is greater than 90%, indoor CO
2concentration is 0.1 ~ 0.2%, and the illuminance of mushroom flower bud initial stage of origination is 50 ~ 100lux, and the illuminance that the middle and later periods occurs mushroom flower bud is 500 ~ 1000lux.
Preferably, in step (1), the batching of medium is cotton seed hulls 40%, wood chip 38%, wheat bran 18%, corn flour 2%, carbonic acid fine particle calcium carbonate 1%, lime 1%, pH value 7.5 ~ 8, water content 60 ~ 70%.
Preferably, the concentration of step (5) mesochite oligosaccharide solution is 0.05g/ml, and the chitosan oligosaccharide solution hydrops in circular groove removes by medium after 1h fully absorbs.
Time not specified, percentage sign all represents percentage by weight.
Beneficial effect of the present invention is:
Utilize chitosan oligosaccharide to the inducing action of seafood mushroom cell, when not affecting output, the quality of seafood mushroom Squaring number and seafood mushroom, break the resting state of the sexual growth of seafood mushroom cell, make seafood mushroom enter sexual vegetative period ahead of time and budding ahead of time, the asexual growth phase of seafood mushroom (i.e. bacteria time) can be made to shorten 30 ~ 35 days.
Embodiment
For the ease of understanding the present invention, below with reference to specific embodiment, the present invention is described more fully.But the present invention can realize in many different forms, is not limited to embodiment described herein.
A kind of chitosan oligosaccharide induction seafood mushroom is buddingged technology, comprises the following steps:
Step one, medium preparation process, the batching of 100kg medium is cotton seed hulls 40kg, wood chip 38kg, wheat bran 18kg, corn flour 2kg, carbonic acid fine particle calcium carbonate 1kg, lime 1kg, pH value 7.5 ~ 8, water content 65%;
Step 2, medium loads bacterium bottle operation, and loaded by medium in the vinyon bottle of 800ml, every bottle of medium siccative is 250g;
Step 3, sterilization process, first needs the cold air in pot all to get rid of, then at temperature bacterium bottle being remained on 121 ~ 128 DEG C, maintains 2.5h, then make bacterium bottle be cooled to less than 30 DEG C;
Step 4, bacterium operation is sent out in inoculation, aseptically carries out strain inoculation to the medium in bacterium bottle, after strain inoculation completes, indoor temperature is controlled within the scope of 22 ~ 25 DEG C, by CO
2concentration remains on the bacterium process that less than 0.4% carries out seafood mushroom;
Step 5, mycelium stimulation urges flower bud operation, medium enters mycelium stimulation and urges flower bud operation after an inoculation bacterium operation, mycelium stimulation urges the method for flower bud operation to be the surrounding of medium scratched except forming circular groove, in bacterium bottle/bacterium bag, implantation concentration is the chitosan oligosaccharide solution of 0.05g/ml, and medium enters the process of urging flower bud after being removed by the chitosan oligosaccharide solution hydrops in circular groove after 1h fully absorbs; The method of flower bud is urged to be that bacterium bottle/bacterium bag is inserted indoor and is hidden by bacterium bottle/bacterium bag with porose plastic film or non-woven fabrics, the temperature of described indoor is 14 ~ 16 DEG C, and humidity is greater than 90%, CO in holding chamber
2concentration is 0.1 ~ 0.2%, and the illuminance of mushroom flower bud initial stage of origination is 50 ~ 100lux, and the illuminance that the middle and later periods occurs mushroom flower bud is 500 ~ 1000lux.
Employing chitosan oligosaccharide induction seafood mushroom is buddingged after technology, break the resting state of the sexual growth of seafood mushroom cell, made seafood mushroom enter sexual vegetative period ahead of time and budding ahead of time, shorten the bacteria time of 30 days, biological transformation ratio reaches 80%, and reduces the production cost of 1%.
Claims (3)
1. chitosan oligosaccharide induction seafood mushroom is buddingged a technology, it is characterized in that, comprises the following steps:
(1) medium preparation process, the batching of medium is cotton seed hulls 35 ~ 45%, wood chip 34 ~ 38%, wheat bran 15 ~ 20%, corn flour 1.5 ~ 3%, carbonic acid fine particle calcium carbonate 0.8 ~ 1.2%, lime 0.8 ~ 1.2%, pH value 7.5 ~ 8, water content 60 ~ 70%;
(2) medium loads bacterium bottle/bacterium bag process, is loaded by medium in the bacterium bottle/bacterium bag of 700 ~ 900ml, and every bottle/every bag culture medium siccative is 240 ~ 260g;
(3) sterilization process, first needs the cold air in pot all to get rid of, then under bacterium bottle/bacterium bag is remained on the temperature of 121 ~ 128 DEG C, maintains 2 ~ 2.5h, then make bacterium bottle/bacterium bag be cooled to less than 30 DEG C;
(4) bacterium operation is sent out in inoculation, aseptically carries out strain inoculation to the medium in bacterium bottle/bacterium bag, after strain inoculation completes, indoor temperature is controlled within the scope of 22 ~ 25 DEG C, by CO
2concentration remains on the bacterium process that less than 0.4% carries out seafood mushroom;
(5) mycelium stimulation urges flower bud operation, its processing method is, the surrounding of the medium sent out after bacterium operation through described inoculation is scratched except forming circular groove, in bacterium bottle/bacterium bag, implantation concentration is the chitosan oligosaccharide solution of 0.03 ~ 0.07g/ml, and medium fully absorbs through 0.8h ~ 1.2h and enters the process of urging flower bud by after the chitosan oligosaccharide solution hydrops removal in circular groove;
The described method of flower bud of urging is, bacterium bottle/bacterium bag is inserted indoor and is hidden by bacterium bottle/bacterium bag with porose plastic film or non-woven fabrics, the temperature of described indoor is 14 ~ 16 DEG C, and humidity is greater than 90%, indoor CO
2concentration is 0.1 ~ 0.2%, and the illuminance of mushroom flower bud initial stage of origination is 50 ~ 100lux, and the illuminance that the middle and later periods occurs mushroom flower bud is 500 ~ 1000lux.
2. chitosan oligosaccharide according to claim 1 induction seafood mushroom is buddingged technology, it is characterized in that, in described step (1), the batching of medium is cotton seed hulls 40%, wood chip 38%, wheat bran 18%, corn flour 2%, carbonic acid fine particle calcium carbonate 1%, lime 1%, pH value 7.5 ~ 8, water content 60 ~ 70%.
3. chitosan oligosaccharide according to claim 1 induction seafood mushroom is buddingged technology, and it is characterized in that, the concentration of described chitosan oligosaccharide solution is 0.05g/ml, and the chitosan oligosaccharide solution hydrops in circular groove removes by medium after 1h fully absorbs.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108575560A (en) * | 2018-04-10 | 2018-09-28 | 山东省农业科学院农业资源与环境研究所 | A kind of cultural method of the beautiful gill fungus of evil spirit |
Citations (7)
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| JPH06116113A (en) * | 1992-10-06 | 1994-04-26 | Gold Kosan Kk | Plant growth regulator |
| JPH06311820A (en) * | 1993-04-30 | 1994-11-08 | Fumio Murata | Cultivation of mushroom and culture base |
| CN1809274A (en) * | 2003-04-22 | 2006-07-26 | 生物农业股份有限公司 | Use of lipo chitooligosaccharides to initiate early flowering and fruit development in plants and related methods and compositions. |
| CN102924179A (en) * | 2012-11-22 | 2013-02-13 | 海南正业中农高科股份有限公司 | Application of chitosan-oligosaccharide containing composition to production of edible fungi |
| CN104261931A (en) * | 2014-08-29 | 2015-01-07 | 江苏省天健生物科技有限公司 | Production method of hypsizygus marmoreus original seed culture medium |
| CN104609954A (en) * | 2015-01-10 | 2015-05-13 | 枞阳县周泽红家庭农场 | Coprinus comatus culture medium containing mushroom dregs and preparation method of coprinus comatus culture medium |
| CN104684871A (en) * | 2012-09-27 | 2015-06-03 | 诺维信生物农业公司 | Compositions and methods for enhancing plant growth |
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2015
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Patent Citations (7)
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| JPH06116113A (en) * | 1992-10-06 | 1994-04-26 | Gold Kosan Kk | Plant growth regulator |
| JPH06311820A (en) * | 1993-04-30 | 1994-11-08 | Fumio Murata | Cultivation of mushroom and culture base |
| CN1809274A (en) * | 2003-04-22 | 2006-07-26 | 生物农业股份有限公司 | Use of lipo chitooligosaccharides to initiate early flowering and fruit development in plants and related methods and compositions. |
| CN104684871A (en) * | 2012-09-27 | 2015-06-03 | 诺维信生物农业公司 | Compositions and methods for enhancing plant growth |
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| CN104261931A (en) * | 2014-08-29 | 2015-01-07 | 江苏省天健生物科技有限公司 | Production method of hypsizygus marmoreus original seed culture medium |
| CN104609954A (en) * | 2015-01-10 | 2015-05-13 | 枞阳县周泽红家庭农场 | Coprinus comatus culture medium containing mushroom dregs and preparation method of coprinus comatus culture medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108575560A (en) * | 2018-04-10 | 2018-09-28 | 山东省农业科学院农业资源与环境研究所 | A kind of cultural method of the beautiful gill fungus of evil spirit |
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Application publication date: 20160302 |