CN105349414A - System and method thereof for supplementing carbon source in spirulina big pool culture - Google Patents
System and method thereof for supplementing carbon source in spirulina big pool culture Download PDFInfo
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- CN105349414A CN105349414A CN201510729102.1A CN201510729102A CN105349414A CN 105349414 A CN105349414 A CN 105349414A CN 201510729102 A CN201510729102 A CN 201510729102A CN 105349414 A CN105349414 A CN 105349414A
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- pipeline
- spirulina
- sodium carbonate
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- carbonic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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Abstract
The invention relates to a system and a method thereof for supplementing a carbon source in spirulina big pool culture. The system for supplementing the carbon source in the spirulina big pool culture comprises a water return pond, a culture pond and a pipeline, and further comprises a carbon dioxide gas supply system and a sodium carbonate dissolving pond, wherein the pipeline is arranged between the water return pond and the culture pond and is provided with a water return pump; the water return pond and the culture pond are connected by virtue of the pipeline; by virtue of the water return pump, returned water of the water return pond is transmitted into the culture pond through the pipeline; the carbon dioxide gas supply system is communicated with the pipeline by virtue of a carbon dioxide gas supply pipeline; and the sodium carbonate dissolving pond is communicated with the pipeline by virtue of a carbon sodium supply pipeline. The system and the method disclosed by the invention have the advantages that the waste of carbon dioxide is reduced, the yield of spirulina is improved, cost is reduced, the concentration of available carbon source in a culture solution is increased, the usage amount of sodium hydrogen carbonate is reduced and the growth rate of the spirulina is accelerated; and the salinity of the culture solution is increased and simultaneously the carbon source is supplemented in cloudy and rainy weather, so that the spirulina is protected from dying due to great change in the salinity.
Description
Technical field
The present invention relates to a kind of technology of big pool cultivated spirulina, be specifically related to system and the method thereof of supplementary carbon source in a kind of spirulina great Chi cultivation.
Background technology
Carbon source is absolutely necessary for the growth carrying out photosynthetic various algae, maintains higher growth velocity and just must provide a large amount of carbon sources.Carbon source in SPIRULINA CULTIVATION process is sodium bicarbonate and carbonic acid gas mainly.
At present, the nutrient solution of domestic SPIRULINA CULTIVATION process can recycle, the carbon source used in SPIRULINA CULTIVATION is sodium bicarbonate and carbonic acid gas mainly, and concrete using method is that the spirulina medium after collection of spirulina being completed backward backwater and gathering is squeezed in culturing pool by pump in the liquid continuing to use and squeezed into carbonic acid gas and simultaneously in great Chi, supplement sodium bicarbonate.But the defect that above-mentioned way exists is: 1. carbonic acid gas is directly squeezed in backwater and caused nutrient solution pH too low, and back pool is not closed, carbonic acid gas is overflowed, and carbon source is wasted, actual little by absorbed dose; 2. supplementing a large amount of sodium bicarbonate during Cyclic culture causes salinity in nutrient solution constantly to raise, and suppresses the growth of spirulina; 3. rainy weather is gathered, great Chi nutrient solution pH is originally just low, photosynthesis can not be carried out, because the membership that adds of carbonic acid gas causes the too low spirulina that causes of nutrient solution pH dead, thus the rainy day can not add carbonic acid gas, and cause nutrient solution salinity altercation excessive to tackle precipitation, need to supplement sodium-chlor and sodium bicarbonate in great Chi.
Summary of the invention
The object of the present invention is to provide system and the method thereof of supplementary carbon source in the spirulina great Chi cultivation that a kind of utilization rate of carbon dioxide is high, production cost is low, the present invention proposes the method for supplementary carbon dioxide and sodium carbonate simultaneously in SPIRULINA CULTIVATION process first, changes method during tradition interpolation carbon source.
Object of the present invention is achieved through the following technical solutions: the system of supplementary carbon source during a kind of spirulina great Chi cultivates, it comprises back pool, culturing pool and be located at back pool, the pipeline with back water pump between culturing pool, described back pool, culturing pool is connected by pipeline, make back water pump that the backwater of back pool is sent into culturing pool by pipeline, it is characterized in that: it also comprises carbonic acid gas airing system and sodium carbonate dissolving tank, described carbonic acid gas airing system is by carbonic acid gas steam line and pipeline communication, sodium carbonate dissolving tank is by sodium carbonate supply line and pipeline communication.
Described carbonic acid gas steam line and sodium carbonate supply line are all connected on the pipeline between back water pump and back pool.Such design makes backwater, carbonic acid gas and sodium carbonate can react to each other more fully through back water pump.
Carbonic acid gas and the sodium carbonate joint first in pipeline is not less than 20m to the duct length between pipe water outlet.Backwater is made to have time enough fully to react with sodium carbonate, carbonic acid gas under turbulent flow.
The stirrer for fully being mixed by the nutrient solution in backwater and culturing pool is provided with in described culturing pool.
A method for supplementary carbon source during spirulina great Chi cultivates, is characterized in that: it comprises the following steps:
1) sodium carbonate is dissolved in sodium carbonate dissolving tank sodium carbonate is reached capacity state;
2) back water pump is opened, simultaneous regulation of carbon dioxide steam line top hole pressure, fully mix under the turbulence state that sodium carbonate, carbonic acid gas, backwater are formed in the duct, then pump in spirulina culture pond, and the pH value of pipe water outlet place, backwater controls as 7.5-8.0;
3) open the stirrer in culturing pool, make to mix with the nutrient solution in culturing pool with the backwater of carbonic acid gas, sodium carbonate, the pH value of solution in culturing pool is controlled between 9.0-9.5.
The present invention is according to the running balance that there are three kinds of carbon source forms in spirulina medium and mutually transform:
K
1, K
2chemical reaction equilibrium constant, when adopting volumetric molar concentration,
K
1＝4.3×10
-7
K
2＝5.6×10
-11
When reaction is in dynamic balance state, CO
2,
the concentration of three is satisfied with following amounts relation:
Three kinds of carbon sources form the balance dynamic mobile mutually transformed, and decide the trend of medium pH value change.
Mentality of designing of the present invention is: in water return pipeline, improve CO simultaneously
2with
concentration, forces molecular balance to generation
direction move, generate certain H+ ion simultaneously, reduce the pH value of backwater.In fact, add and can utilize carbon source
concentration, when backwater flows into after in SPIRULINA CULTIVATION great Chi, the concentration due to OH-in great Chi is about 10-4mol/LpH10, can neutralize the H+ ion produced primarily of above-mentioned balance 1, impels balance to continue towards generation
direction move, make the residue CO do not reacted in water return pipeline
2continue reaction; Meanwhile, the pH value of cultivating in great Chi can decrease to some degree.
Compared to prior art, the invention has the advantages that: 1) sodium carbonate is combined to spirulina medium supplementary carbon source with carbonic acid gas by the present invention, decreases the waste of carbonic acid gas; 2) invention increases the output of spirulina; 3) present invention reduces cost, increase in nutrient solution and can utilize carbon source concentration, reduce the usage quantity of sodium bicarbonate, increase growth of spirulina platensis speed; 4) the present invention can improve the salinity simultaneously supplementary carbon source of nutrient solution at rainy weather, avoid spirulina due to salinity altercation excessive and dead.
Accompanying drawing explanation
Fig. 1 is the structural representation of supplementary carbon source system of the present invention.
Label declaration: 1 back pool, 2 culturing pools, 3 sodium carbonate dissolving tanks, 4 back water pumps, 4-1 pipeline, 5 carbonic acid gas steam lines, 6 stirrers, 7 sodium carbonate supply lines.
Embodiment
Below in conjunction with Figure of description and embodiment, content of the present invention is described in detail:
As shown in Figure 1: the system of supplementary carbon source during a kind of spirulina great Chi cultivates, it comprises back pool 1, culturing pool 2 and be located at back pool 1, the pipeline 4-1 with back water pump 4 between culturing pool 2, described back pool 1, culturing pool 2 is connected by pipeline 4-1, make back water pump 4 that the backwater of back pool 1 is sent into culturing pool 2 by pipeline 4-1, it also comprises carbonic acid gas airing system 5 and sodium carbonate dissolving tank 3, described carbonic acid gas airing system 5 is communicated with pipeline 4-1 by carbonic acid gas steam line 5, sodium carbonate dissolving tank 3 is communicated with pipeline 4-1 by sodium carbonate supply line 7.
Described carbonic acid gas steam line 5 and sodium carbonate supply line 7 are all connected on the pipeline 4-1 between back water pump 4 and back pool 1.
Duct length between carbonic acid gas and the sodium carbonate joint first in pipeline 4-1 to pipeline 4-1 water outlet is not less than 20m.
The stirrer 6 for fully being mixed by the nutrient solution in backwater and culturing pool 2 is provided with in described culturing pool 2.
Embodiment one:
A method for supplementary carbon source during spirulina great Chi cultivates, it comprises the following steps:
1) sodium carbonate is dissolved in sodium carbonate dissolving tank 3 sodium carbonate is reached capacity state;
2) back water pump 4 is opened, simultaneous regulation of carbon dioxide steam line 5 top hole pressure, fully mix under the turbulence state that sodium carbonate, carbonic acid gas, backwater are formed in pipeline, then pump in spirulina culture pond 2, and the pH value of pipeline 4-1 water outlet, backwater controls to be 7.5;
3) open the stirrer 6 in culturing pool 2, the backwater with carbonic acid gas, sodium carbonate is mixed with the nutrient solution in culturing pool 2, and it is being 9.5 that the pH value of solution in culturing pool 2 is controlled.
Embodiment two:
A method for supplementary carbon source during spirulina great Chi cultivates, it comprises the following steps:
1) sodium carbonate is dissolved in sodium carbonate dissolving tank 3 sodium carbonate is reached capacity state;
2) back water pump 4 is opened, simultaneous regulation of carbon dioxide steam line 5 top hole pressure, fully mix under the turbulence state that sodium carbonate, carbonic acid gas, backwater are formed in pipeline, then pump in spirulina culture pond 2, and the pH value of pipeline 4-1 water outlet, backwater controls to be 7.8;
3) open the stirrer 6 in culturing pool 2, the backwater with carbonic acid gas, sodium carbonate is mixed with the nutrient solution in culturing pool 2, it is 9.3 that the pH value of solution in culturing pool 2 is controlled.
Embodiment three:
A method for supplementary carbon source during spirulina great Chi cultivates, is characterized in that: it comprises the following steps:
1) sodium carbonate is dissolved in sodium carbonate dissolving tank 3 sodium carbonate is reached capacity state;
2) back water pump 4 is opened, simultaneous regulation of carbon dioxide steam line 5 top hole pressure, fully mix under the turbulence state that sodium carbonate, carbonic acid gas, backwater are formed in pipeline, then pump in spirulina culture pond 2, and the pH value of pipeline 4-1 water outlet, backwater controls to be 8.0;
3) open the stirrer 6 in culturing pool 2, the backwater with carbonic acid gas, sodium carbonate is mixed with the nutrient solution in culturing pool 2, and making the pH value of solution in culturing pool 2 control at pH is 9.0.
Above 3 embodiments are the test carried out in the plant having 120,000 square metres of spirulina culture ponds one month, contriver traditional method is squeezed into carbonic acid gas carry out carbon source supplement test in contrast, find: carbonic acid gas directly adds in pipeline by traditional method, pipeline 4-1 water outlet pH is 5.5-6.0, the pressure of the carbonic acid gas steam line that above 3 embodiments adopt and traditional method carbonic acid gas steam line pressure used are consistent, and above 3 embodiment backwater exit pH are 7.5-8.0, also obtain experimental result in addition:
Table 1. the present invention 3 embodiments and controlled trial test the result of month
The present invention is for traditional carbon source compensation process as known from Table 1, and the present invention can not only improve the utilization ratio of carbonic acid gas, improve the output of spirulina, and reduces the usage quantity of sodium bicarbonate, increases growth of spirulina platensis speed.
Claims (5)
1. a spirulina great Chi cultivate in the system of supplementary carbon source, it comprises back pool (1), culturing pool (2) and be located at back pool (1), the pipeline with back water pump (4) (4-1) between culturing pool (2), described back pool (1), culturing pool (2) is connected by pipeline (4-1), make back water pump (4) that the backwater of back pool (1) is sent into culturing pool (2) by pipeline (4-1), it is characterized in that: it also comprises carbonic acid gas airing system and sodium carbonate dissolving tank (3), described carbonic acid gas airing system is communicated with pipeline (4-1) by carbonic acid gas steam line (5), sodium carbonate dissolving tank (3) is communicated with pipeline (4-1) by sodium carbonate supply line (7).
2. spirulina great Chi according to claim 1 cultivate in the system of supplementary carbon source, it is characterized in that: described carbonic acid gas steam line (5) and sodium carbonate supply line (7) are all connected on the pipeline (4-1) between back water pump (4) and back pool (1).
3. spirulina great Chi according to claim 1 and 2 cultivate in the system of supplementary carbon source, it is characterized in that: the duct length between carbonic acid gas and the joint first of sodium carbonate in pipeline (4-1) to pipeline (4-1) water outlet is not less than 20m.
4. during the spirulina great Chi according to claims 1 to 3 any one cultivates, the system of supplementary carbon source, is characterized in that: be provided with the stirrer (6) for fully being mixed by the nutrient solution in backwater and culturing pool (2) in described culturing pool (2).
5. spirulina great Chi cultivate in the method for supplementary carbon source, it is characterized in that: it comprises the following steps:
1) sodium carbonate is dissolved in sodium carbonate dissolving tank (3) and sodium carbonate is reached capacity state;
2) back water pump (4) is opened, simultaneous regulation of carbon dioxide steam line (5) top hole pressure, fully mix under the turbulence state that sodium carbonate, carbonic acid gas, backwater are formed in pipeline (4-1), pump into again in spirulina culture pond (2), and the pH value of pipeline (4-1) water outlet, backwater controls as 7.5-8.0;
3) stirrer (6) in culturing pool (2) is opened, backwater with carbonic acid gas, sodium carbonate is mixed with the nutrient solution in culturing pool (2), the pH value of solution in culturing pool (2) is controlled between 9.0-9.5.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510729102.1A CN105349414A (en) | 2015-10-30 | 2015-10-30 | System and method thereof for supplementing carbon source in spirulina big pool culture |
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| CN201510729102.1A CN105349414A (en) | 2015-10-30 | 2015-10-30 | System and method thereof for supplementing carbon source in spirulina big pool culture |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486660A (en) * | 2018-11-08 | 2019-03-19 | 浙江大学 | A kind of carbon dioxide absorption tower formula bioreactor and both culturing microalgae method |
| CN110917866A (en) * | 2019-12-16 | 2020-03-27 | 四川轻化工大学 | Carbon dioxide gas fixing device for microalgae culture |
| CN114752502A (en) * | 2022-04-29 | 2022-07-15 | 云南绿A生物产业园有限公司 | Culture method and system for improving growth rate of spirulina by comprehensively utilizing carbon source |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101066807A (en) * | 2007-05-31 | 2007-11-07 | 天津禄升海洋生物科技有限公司 | Sewage treating micro algae apparatus and process |
| CN101654313A (en) * | 2009-09-15 | 2010-02-24 | 哈尔滨工业大学水资源国家工程研究中心有限公司 | Method for utilizing advanced oxidation for carrying out pretreatment on sewage and culturing engineering microalgae for carrying out sewage deep treatment and carbon dioxide emission reduction |
| CN102304463A (en) * | 2011-09-01 | 2012-01-04 | 武汉凯迪工程技术研究总院有限公司 | Spirulina culture system and method based on silica white production system |
| CN102382755A (en) * | 2011-07-28 | 2012-03-21 | 中国科学院青岛生物能源与过程研究所 | Microalgae culturing device and microalgae culturing method |
| CN202246658U (en) * | 2011-09-28 | 2012-05-30 | 福清市新大泽螺旋藻有限公司 | Special device for improving utilization rate of carbon dioxide in process of cultivating microalgae |
-
2015
- 2015-10-30 CN CN201510729102.1A patent/CN105349414A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101066807A (en) * | 2007-05-31 | 2007-11-07 | 天津禄升海洋生物科技有限公司 | Sewage treating micro algae apparatus and process |
| CN101654313A (en) * | 2009-09-15 | 2010-02-24 | 哈尔滨工业大学水资源国家工程研究中心有限公司 | Method for utilizing advanced oxidation for carrying out pretreatment on sewage and culturing engineering microalgae for carrying out sewage deep treatment and carbon dioxide emission reduction |
| CN102382755A (en) * | 2011-07-28 | 2012-03-21 | 中国科学院青岛生物能源与过程研究所 | Microalgae culturing device and microalgae culturing method |
| CN102304463A (en) * | 2011-09-01 | 2012-01-04 | 武汉凯迪工程技术研究总院有限公司 | Spirulina culture system and method based on silica white production system |
| CN202246658U (en) * | 2011-09-28 | 2012-05-30 | 福清市新大泽螺旋藻有限公司 | Special device for improving utilization rate of carbon dioxide in process of cultivating microalgae |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486660A (en) * | 2018-11-08 | 2019-03-19 | 浙江大学 | A kind of carbon dioxide absorption tower formula bioreactor and both culturing microalgae method |
| CN110917866A (en) * | 2019-12-16 | 2020-03-27 | 四川轻化工大学 | Carbon dioxide gas fixing device for microalgae culture |
| CN114752502A (en) * | 2022-04-29 | 2022-07-15 | 云南绿A生物产业园有限公司 | Culture method and system for improving growth rate of spirulina by comprehensively utilizing carbon source |
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Application publication date: 20160224 |