CN105347885A - Bottled wood chip bacterium culture medium - Google Patents

Abstract

The invention discloses a bottled wood chip bacterium culture medium. 100% of the bottled wood chip bacterium culture medium comprises, by weight, 80% of wood chip, 10% of saw dust, 7.5% of wheat bran, 1% of gypsum, 1% of glucose, and 0.5% of magnesium sulfate. A preparation method is simple, is high in peculiarity, is convenient, and is high in practicality. The bottled wood chip bacterium culture medium is capable of providing farmers with convenience in edible mushrooms production process; operation of the bacterium production using the bottled wood chip bacterium culture medium is simple; bacterium production amount is large, and survival rate is high; the properties of obtained bacterium are stable, variation is not easily caused, bacterium growth speed is high; the bottled wood chip bacterium culture medium is capable of resisting storage, aging is not easily caused, production cost is reduced, pollution is reduced, and bacterium prepared with the bottled wood chip bacterium culture medium possesses storage resistance, and is portable; operation is simple; and strain characteristics are stable.

Description

A kind of bottled wooden unit bacterium culture medium

Technical field

The present invention relates to breed of edible fungus technical field, be specifically related to a kind of bottled wooden unit bacterium culture medium.

Background technology

Edible mushrooms refers to that sporophore is very large, macro fungi that can be edible, is commonly referred to as mushroom.The known edible mushrooms of China has kind more than 350, wherein belongs to Basidiomycotina more, common are: mushroom, straw mushroom, mushroom, auricularia auriculajudae, white fungus, hedgehog hydnum, dictyophora phalloidea, Trichotoma matsutake, dried mushroom, russule, glossy ganoderma, Chinese caterpillar fungus, truffle, lark and bolete etc.; Minority belongs to Ascomycotina, Qi Zhongyou: morel, saddle fungus, ferfas etc.It is nutritious that edible mushrooms has bacterium, high protein, lower fat, the advantages such as delicious flavour have become one of protective foods of people's eating, in recent years, the edible mushrooms cause of China just advances with unprecedented scale, production technology also improves a lot, for improving edible mushrooms seed output and quality, people are from bacterial classification, substratum nutriment, send out bacterium and management of producing mushroom technology to tackle key problems, achieve very much progress, the selection of substratum determines the growth efficiency of edible mushrooms to a certain extent, in addition, the selection of substratum determines production process whether environmental protection too, whether reach the object of energy-saving and emission-reduction.

Tradition culture medium of edible fungus generally comprises following raw material: wood chip, wheat skin, brown sugar and gypsum etc., the every double centner substratum of this culture medium of edible fungus prepared produces edible mushrooms about 6.5 kilograms, in base, raw material is few, lack nutrient raw material, growth is slow, inoculation quality is low, causes inoculation efficiency low, and bacterial classification output is and then reduced.

Summary of the invention

The object of the invention is to provide a kind of bottled wooden unit bacterium culture medium, and it can solve problem existing in background technology effectively.

A kind of bottled wooden unit bacterium culture medium, comprise following composition wooden unit, sawdust, wheat bran, gypsum and white sugar, wherein the scope of various composition is:

Wherein the summation of various composition is 100% (weight).

The shape of described wooden unit can be any one in rectangular parallelepiped, square, spheroid or right cylinder.

Described wooden unit is broad-leaved wooden unit.

A making method for above-mentioned substratum, specifically comprises following steps:

(1). first wooden unit is soaked 6 hours in potassium dihydrogen phosphate, described potassium dihydrogen phosphate is that the potassium primary phosphate that clear water adds 0.3% soaks 6 hours;

(2). then by the wooden unit through soaking and sawdust, wheat bran, gypsum, glucose, bottles after magnesium sulfate stirs;

(3). then cover tightly bottle cap and carry out sterilizing works;

(4). finally inoculate, fermentation, check, detect, the processes such as finished product packing;

As the preferred version of above-mentioned steps (2), the wooden unit soaked directly can be mixed wheat bran bottling.

Owing to have employed above technical scheme, the present invention has following beneficial effect: preparation method is simple, there is extraordinary uniqueness, practicality fast and easily, allow peasant household can be more handy in the process of producing edible mushrooms, convenient and swift, handy, the bacterial classification that this substratum is produced has simple to operate, bacterial classification amount is large, surviving rate is high, bacterial classification kind is stable not easily to make a variation, send out bacterium speed fast, storage endurance is not easily aging, reduce production cost, decreasing pollution, the bacterial classification that basal culture medium is produced simultaneously has storage endurance, the advantages such as simple to operate kind of property easy to carry is stable, use basal culture medium bacterial classification, be suitable for connecing sawdust medium and with grip for ice cream, and the secondary bacterium culture medium of plastic bag such as disposable chopsticks, connect bacterium.

Embodiment

The technique means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below the technical scheme in the embodiment of the present invention being clearly and completely described.

Embodiment 1

A kind of bottled wooden unit bacterium culture medium, comprise following composition wooden unit, sawdust, wheat bran, gypsum and white sugar, wherein the scope of various composition is:

Wherein the summation of various composition is 100% (weight).

The shape of described wooden unit can be any one in rectangular parallelepiped, square, spheroid or right cylinder.

Described wooden unit is broad-leaved wooden unit.

Embodiment 2

A making method for substratum described in process embodiment 1, specifically comprises following steps:

(1). first wooden unit is soaked 6 hours in potassium dihydrogen phosphate, described potassium dihydrogen phosphate is that the potassium primary phosphate that clear water adds 0.3% soaks 6 hours;

(2). then by the wooden unit through soaking and sawdust, wheat bran, gypsum, glucose, bottles after magnesium sulfate stirs;

(3). then cover tightly bottle cap and carry out sterilizing works;

(4). finally inoculate, fermentation, check, detect, the processes such as finished product packing;

As the preferred version of above-mentioned steps (2), the wooden unit soaked directly can be mixed wheat bran bottling.

The bacterial classification that the substratum that the present embodiment is made is produced has storage endurance, the advantages such as simple to operate kind of property easy to carry is stable, use basal culture medium bacterial classification, are suitable for connecing sawdust medium and with grip for ice cream, and the secondary bacterium culture medium of plastic bag such as disposable chopsticks, connect bacterium.

The Novel bottled wooden unit bacterium culture medium gone out according to embodiment 1 and embodiment 2 processing and fabricating has following effect:

1. bacterial classification amount is large, and to than test tube PDA substratum in the past, one bottle of Novel bottled wooden unit bacterium culture medium bacterial classification is equivalent to the inoculum size that 10-30 props up specification (2.2cm × 20cm) test tube PDA substratum;

2. storage endurance is not easily aging, and the quality guaranteed period of test tube PDA substratum is generally 18 days to 30 days, more than 6 times of the quality guaranteed period of Novel bottled wooden unit bacterium culture medium bacterial classification, to be 200 day quality guaranteed period be test tube PDA substratum;

3. surviving rate bacterium occurred frequently speed is fast: the surviving rate of test tube PDA substratum is 70 to 85% and the connecing bacterium success ratio and can reach more than 99% of Novel bottled wooden unit bacterium culture medium bacterial classification, and surviving rate is 1.2 times of test tube PDA substratum.Sending out bacterium with the original seed that test tube PDA substratum connects bacterium is cover with for 45-60 days, and the original seed that useful " Novel bottled wooden unit bacterium culture medium " bacterial classification is sent out is 25 days---within 35 days, just can cover with, and cell age is unanimous between the higher and lower levels, so this last important breakthrough that is edible mushrooms history

Embodiment 3

First ready-made secondary bacterium culture medium bag normal-pressure sterilization 100 degree is kept 8-10 hour, take the dish out of the pot naturally cooling, time in bag within temperature 20 degree, carry out attaching bacterium box sterilization, carry out wiping before connecing bacterium with 75% alcohol to tweezers, rear spirit lamp burns tweezers 1 minute, then the substratum produced according to embodiment 1-2 is turned on bacterial classification bottle cap by sawdust removing above, can inoculate, in each bag, put one piece of wooden unit bacterial classification, then be stoppered continuous plug and connect bacterium successively.Connect the bacterial classification that bacterium is remaining, tightening after must burning burning cap with spirit lamp, use in order to next time, all processes all will aseptically operate at every turn.

Send out bacterium temperature after producing secondary bacterium with this substratum strain transfer not easily too high, preferably control to carry out sending out bacterium at 22 DEG C-25 DEG C.Remarks; Each use is left to open connecing bacterium and can not preserving for a long time using unspent bacterial classification in 72 hours of lid.

Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art should be appreciated that it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.

Claims (4)

1. a bottled wooden unit bacterium culture medium, it is characterized in that comprising following composition wooden unit, sawdust, wheat bran, gypsum and white sugar, wherein the scope of various composition is:

2. the bottled wooden unit bacterium culture medium of one according to claim 1, is characterized in that the shape of described wooden unit can be any one in rectangular parallelepiped, square, spheroid or right cylinder.

3. the bottled wooden unit bacterium culture medium of one according to claim 1, is characterized in that described wooden unit is broad-leaved wooden unit.

4. a making method for the bottled wooden unit bacterium culture medium as described in claim 1-3, is characterized in that specifically comprising following steps:

(1). first wooden unit is soaked 6 hours in potassium dihydrogen phosphate, described potassium dihydrogen phosphate is that the potassium primary phosphate that clear water adds 0.3% soaks 6 hours;

(2). then by the wooden unit through soaking and sawdust, wheat bran, gypsum, glucose, bottles after magnesium sulfate stirs;

(3). then cover tightly bottle cap and carry out sterilizing works;

(4). finally inoculate, fermentation, check, detect, the processes such as finished product packing;

As the preferred version of above-mentioned steps (2), the wooden unit soaked directly can be mixed wheat bran bottling.