CN105340412A - Preparation method for soybean nodulation pathogenic-bacterium inhibitor - Google Patents
Preparation method for soybean nodulation pathogenic-bacterium inhibitor Download PDFInfo
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- CN105340412A CN105340412A CN201510636730.5A CN201510636730A CN105340412A CN 105340412 A CN105340412 A CN 105340412A CN 201510636730 A CN201510636730 A CN 201510636730A CN 105340412 A CN105340412 A CN 105340412A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- Agronomy & Crop Science (AREA)
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- Biotechnology (AREA)
- Microbiology (AREA)
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- Pest Control & Pesticides (AREA)
- Beans For Foods Or Fodder (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention relates to a preparation method for a soybean nodulation pathogenic-bacterium inhibitor and belongs to the field of agriculture. Aiming at the current problem that the development of a soybean root system is harmed, the absorption of crops to moisture and nutrients is affected and the production reduction and plant death of soybeans are caused is mainly caused due to the fact that soybean plant roots are susceptible, the invention provides a method for extracting a soybean plant root secretion product as the pathogenic-bacterium inhibitor through culturing soybean plants, the growth and proliferation of pathogenic bacteria of soybean roots are effectively inhibited, and the yield of soybeans per mu is increased by 5-10% compared with that of the soybeans where a soybean nodulation pathogenic-bacterium inhibitor dilute solution is not applied.
Description
Technical field
The present invention relates to a kind of preparation method of soybean nodulation germ inhibitor, belong to agriculture field.
Background technology
The nutritive value of soybean is very high, is most important oil crop and high protein cereal crops in the world.In the dry matter contained by soybean kernel, protein accounts for 40%, and fat accounts for 20%, and carbohydrate accounts for 35%, and ash content accounts for 5%.The protein content of soybean exceeds more than 10 percentage points than other beans, and the 3-5 being general cereal doubly, is one of main dietary protein origin of the mankind.Soybean not only protein content is high, and protein quality is good, and amino acid forms closely human body needs, is the high-quality protein of human diet Middle nutrition balance.Soybean is also containing more fat, and oil quality is excellent.In fatty acid component flat greatly, the ratio of unsaturated fatty acid, up to 80 ~ 88%, is conducive to reducing cholesterol, preventing hypertension and the angiocardiopathy in blood.In addition, soybean is also containing some micro constitutents, and as oligosaccharide, isoflavones, saponin, phosphatide, vitamin E etc., these materials have special health-care effect to human body, have caused the attention of people.The purposes of soybean is very extensive, and the food made of soybean and chemical products have thousands of kinds.Except oil expression, soybean is also for making the numerous food such as bean curd, soya-bean milk, bean sprouts, fermented bean curd, soy sauce, thick broad-bean sauce, natto, red shellfish.Soybean oil is except edible, and all right making artificial cream, produces soap, glycerine, waterproofing agent, paint, lubrication wet goods.By the important source material that the protein isolate of Soybean production and protein concentrate are food industry.Dregs of beans is the main source of forage protein, very important to the development of livestock breeding.Along with the enhancing of health of people idea and the development of food chemistry industry, soybean prod also by appearing in the life of people more and more, plays an increasingly important role.
But soybean is at plantation and growing period, soybean nodulation easily infects, cause main harm soybean root system to grow, affect the absorption of crop to moisture and nutriment, make that the root of soybean plant strain is heavy, root nodule is heavy, impact that nitrogenase activity and side radical are all subject in various degree.Diseased plant 100-grain weight reduces by 1 ~ 3g than healthy plant, and the general underproduction 10 ~ 30%, can reach 60% time serious.If with soy bean cyst roundworm and the morbidity that combines, harm can be more serious, can cause the obvious depauperation of acrial part plant, even whole withered deaths, so need a kind of inhibitor of effective suppression soybean root pathogen growth breeding necessary.
Summary of the invention
The technical problem that the present invention mainly solves: easily infect for current soybean plant strain root, main harm soybean root system is caused to grow, affect the absorption of crop to moisture and nutriment, cause the problem of the soybean underproduction and plant death, provide a kind of by cultivating soybean plant strain, extract soybean plant strain root secretory product as the method for germ inhibitor, restrained effectively the Growth and reproduction of soybean root pathogen.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention:
(1) full grains are chosen, uniform soya seeds, be placed on after soaking 2 ~ 3h in deionized water, take out and use mass concentration be 10% hydrogenperoxide steam generator sterilization 20 ~ 30min, after process to be sterilized completes, with deionized water rinsing 3 ~ 5 times, be placed on and constantly pass in the saturated calcium sulphate soln of oxygen, soak 3 ~ 5h;
(2) treat that seed is in the saturated calcium sulphate soln passing into oxygen, when imbibition to volume is 1.5 times of original volume, to be taken out and the diameter be placed in distilled water is wetting is on 7cm filter paper, often open filter paper and place 3 ~ 5 seeds, the filter paper that placed seed is placed in dark vernalization, when seed shows money or valuables one carries unintentionally, be placed in the quartz sand matrix of sterilizing at 170 ~ 190 DEG C, every 3 days to pressing the amount of 3 strain 10mL to its quantitative watering, until its growth final singling after 10 ~ 15 days, every basin stays seedling 10 strain;
(3) count by weight, choose the nitrate of lime of the deionized water of 35 ~ 65 parts, the potassium nitrate of 15 ~ 25 parts, the magnesium sulfate of 5 ~ 15 parts and 15 ~ 25 parts, it is mixed in order it to dissolve completely, configure to obtain plant nutrition liquid, by every basin 100 ~ 150mL plant nutrition liquid, every day, 12 noon was to its fertilizer treatment, was cultured to the colored pod phase;
(4) treat that soybean soybean plant strain is cultured to colored pod after date, after cultivating 2 ~ 3h under being placed on sunlight in morning, with deionized water washing plant root and the quartz sand of 3 ~ 5L, remove the nutrient ion of the quartz sand absorption of root system of plant and non-root exudates, subsequently, by its illumination cultivation 5 ~ 6h under original condition;
(5) after having cultivated under original condition, with 2L deionized water drip washing plant root, and collect leacheate in beaker, and be 10:1 by leacheate and microbial inhibitor mass ratio, add isothiazolinone in above-mentioned leacheate, and at being placed on 40 ~ 45 DEG C, 1/2 of the most original volume of rotary evaporation being vacuumized to it and can be prepared into a kind of soybean nodulation germ inhibitor.
Application of the present invention: get 2.5g soybean nodulation germ inhibitor and be dissolved in 100mL deionized water, be prepared into soybean nodulation germ inhibitor dilution, water 100mL to bean plant sprinkling soybean nodulation germ inhibitor dilution by every strain every day, with the soybean of gathering in the crops in the maturing stage than the soybean of not spraying soybean nodulation germ inhibitor dilution, output increased 5 ~ 10%.
The invention has the beneficial effects as follows: soybean nodulation germ inhibitor fungistatic effect of the present invention is remarkable, and every per mu yield improves 5 ~ 10% than the soybean yields not applying soybean nodulation germ inhibitor dilution.
Embodiment
First full grains are chosen, uniform soya seeds, be placed on after soaking 2 ~ 3h in deionized water, take out and use mass concentration be 10% hydrogenperoxide steam generator sterilization 20 ~ 30min, after process to be sterilized completes, with deionized water rinsing 3 ~ 5 times, be placed on and constantly pass in the saturated calcium sulphate soln of oxygen, soak 3 ~ 5h; Treat that seed is in the saturated calcium sulphate soln passing into oxygen, when imbibition to volume is 1.5 times of original volume, to be taken out and the diameter be placed in distilled water is wetting is on 7cm filter paper, often open filter paper and place 3 ~ 5 seeds, the filter paper that placed seed is placed in dark vernalization, when seed shows money or valuables one carries unintentionally, be placed in the quartz sand matrix of sterilizing at 170 ~ 190 DEG C, every 3 days to pressing the amount of 3 strain 10mL to its quantitative watering, until its growth final singling after 10 ~ 15 days, every basin stays seedling 10 strain; Count by weight, choose the nitrate of lime of the deionized water of 35 ~ 65 parts, the potassium nitrate of 15 ~ 25 parts, the magnesium sulfate of 5 ~ 15 parts and 15 ~ 25 parts, it is mixed in order it to dissolve completely, configure to obtain plant nutrition liquid, by every basin 100 ~ 150mL plant nutrition liquid, every day, 12 noon was to its fertilizer treatment, was cultured to the colored pod phase; Treat that soybean soybean plant strain is cultured to colored pod after date, after cultivating 2 ~ 3h under being placed on sunlight in morning, with deionized water washing plant root and the quartz sand of 3 ~ 5L, remove the nutrient ion of the quartz sand absorption of root system of plant and non-root exudates, subsequently, by its illumination cultivation 5 ~ 6h under original condition; After having cultivated under original condition, with 2L deionized water drip washing plant root, and collect leacheate in beaker, and be 10:1 by leacheate and microbial inhibitor mass ratio, add isothiazolinone in above-mentioned leacheate, and at being placed on 40 ~ 45 DEG C, 1/2 of the most original volume of rotary evaporation being vacuumized to it and can be prepared into a kind of soybean nodulation germ inhibitor.
Example 1
First full grains are chosen, uniform soya seeds, be placed on after soaking 2h in deionized water, take out and use mass concentration be 10% hydrogenperoxide steam generator sterilization 20min, after process to be sterilized completes, with deionized water rinsing 3 times, be placed on and constantly pass in the saturated calcium sulphate soln of oxygen, soak 3h; Treat that seed is in the saturated calcium sulphate soln passing into oxygen, when imbibition to volume is 1.5 times of original volume, to be taken out and the diameter be placed in distilled water is wetting is on 7cm filter paper, often open filter paper and place 3 seeds, the filter paper that placed seed is placed in dark vernalization, when seed shows money or valuables one carries unintentionally, be placed in the quartz sand matrix of sterilizing at 170 DEG C, every 3 days to pressing the amount of 3 strain 10mL to its quantitative watering, until its growth final singling after 10 days, every basin stays seedling 10 strain; Count by weight, choose the nitrate of lime of the deionized water of 35 parts, the potassium nitrate of 25 parts, the magnesium sulfate of 15 parts and 25 parts, it is mixed in order it to dissolve completely, configure to obtain plant nutrition liquid, by every basin 100mL plant nutrition liquid, every day, 12 noon was to its fertilizer treatment, was cultured to the colored pod phase; Treat that soybean soybean plant strain is cultured to colored pod after date, be placed on after cultivating 2h under sunlight morning, with deionized water washing plant root and the quartz sand of 3L, remove the nutrient ion of the quartz sand absorption of root system of plant and non-root exudates, subsequently, by its illumination cultivation 5h under original condition; After having cultivated under original condition, with 2L deionized water drip washing plant root, and collect leacheate in beaker, and be 10:1 by leacheate and microbial inhibitor mass ratio, add isothiazolinone in above-mentioned leacheate, and at being placed on 40 DEG C, 1/2 of the most original volume of rotary evaporation being vacuumized to it and can be prepared into a kind of soybean nodulation germ inhibitor.Getting 2.5g soybean nodulation germ inhibitor is dissolved in 100mL deionized water, be prepared into soybean nodulation germ inhibitor dilution, water 100mL to bean plant sprinkling soybean nodulation germ inhibitor dilution by every strain every day, with the soybean of gathering in the crops in the maturing stage than the soybean of not spraying soybean nodulation germ inhibitor dilution, output increased 5%.
Example 2
First full grains are chosen, uniform soya seeds, be placed on after soaking 2.5h in deionized water, take out and use mass concentration be 10% hydrogenperoxide steam generator sterilization 25min, after process to be sterilized completes, with deionized water rinsing 4 times, be placed on and constantly pass in the saturated calcium sulphate soln of oxygen, soak 4h; Treat that seed is in the saturated calcium sulphate soln passing into oxygen, when imbibition to volume is 1.5 times of original volume, to be taken out and the diameter be placed in distilled water is wetting is on 7cm filter paper, often open filter paper and place 4 seeds, the filter paper that placed seed is placed in dark vernalization, when seed shows money or valuables one carries unintentionally, be placed in the quartz sand matrix of sterilizing at 180 DEG C, every 3 days to pressing the amount of 3 strain 10mL to its quantitative watering, until its growth final singling after 12 days, every basin stays seedling 10 strain; Count by weight, choose the nitrate of lime of the deionized water of 40 parts, the potassium nitrate of 20 parts, the magnesium sulfate of 15 parts and 25 parts, it is mixed in order it to dissolve completely, configure to obtain plant nutrition liquid, by every basin 125mL plant nutrition liquid, every day, 12 noon was to its fertilizer treatment, was cultured to the colored pod phase; Treat that soybean soybean plant strain is cultured to colored pod after date, be placed on after cultivating 2.5h under sunlight morning, with deionized water washing plant root and the quartz sand of 4L, remove the nutrient ion of the quartz sand absorption of root system of plant and non-root exudates, subsequently, by its illumination cultivation 5.5h under original condition; After having cultivated under original condition, with 2L deionized water drip washing plant root, and collect leacheate in beaker, and be 10:1 by leacheate and microbial inhibitor mass ratio, add isothiazolinone in above-mentioned leacheate, and at being placed on 43 DEG C, 1/2 of the most original volume of rotary evaporation being vacuumized to it and can be prepared into a kind of soybean nodulation germ inhibitor.Getting 2.5g soybean nodulation germ inhibitor is dissolved in 100mL deionized water, be prepared into soybean nodulation germ inhibitor dilution, water 100mL to bean plant sprinkling soybean nodulation germ inhibitor dilution by every strain every day, with the soybean of gathering in the crops in the maturing stage than the soybean of not spraying soybean nodulation germ inhibitor dilution, output increased 8%.
Example 3
First full grains are chosen, uniform soya seeds, be placed on after soaking 3h in deionized water, take out and use mass concentration be 10% hydrogenperoxide steam generator sterilization 30min, after process to be sterilized completes, with deionized water rinsing 5 times, be placed on and constantly pass in the saturated calcium sulphate soln of oxygen, soak 5h; Treat that seed is in the saturated calcium sulphate soln passing into oxygen, when imbibition to volume is 1.5 times of original volume, to be taken out and the diameter be placed in distilled water is wetting is on 7cm filter paper, often open filter paper and place 5 seeds, the filter paper that placed seed is placed in dark vernalization, when seed shows money or valuables one carries unintentionally, be placed in the quartz sand matrix of sterilizing at 190 DEG C, every 3 days to pressing the amount of 3 strain 10mL to its quantitative watering, until its growth final singling after 15 days, every basin stays seedling 10 strain; Count by weight, choose the nitrate of lime of the deionized water of 65 parts, the potassium nitrate of 15 parts, the magnesium sulfate of 5 parts and 15 parts, it is mixed in order it to dissolve completely, configure to obtain plant nutrition liquid, by every basin 150mL plant nutrition liquid, every day, 12 noon was to its fertilizer treatment, was cultured to the colored pod phase; Treat that soybean soybean plant strain is cultured to colored pod after date, be placed on after cultivating 3h under sunlight morning, with deionized water washing plant root and the quartz sand of 5L, remove the nutrient ion of the quartz sand absorption of root system of plant and non-root exudates, subsequently, by its illumination cultivation 6h under original condition; After having cultivated under original condition, with 2L deionized water drip washing plant root, and collect leacheate in beaker, and be 10:1 by leacheate and microbial inhibitor mass ratio, add isothiazolinone in above-mentioned leacheate, and at being placed on 45 DEG C, 1/2 of the most original volume of rotary evaporation being vacuumized to it and can be prepared into a kind of soybean nodulation germ inhibitor.Getting 2.5g soybean nodulation germ inhibitor is dissolved in 100mL deionized water, be prepared into soybean nodulation germ inhibitor dilution, water 100mL to bean plant sprinkling soybean nodulation germ inhibitor dilution by every strain every day, with the soybean of gathering in the crops in the maturing stage than the soybean of not spraying soybean nodulation germ inhibitor dilution, output increased 10%.
Claims (1)
1. a preparation method for soybean nodulation germ inhibitor, is characterized in that concrete preparation process is:
(1) full grains are chosen, uniform soya seeds, be placed on after soaking 2 ~ 3h in deionized water, take out and use mass concentration be 10% hydrogenperoxide steam generator sterilization 20 ~ 30min, after process to be sterilized completes, with deionized water rinsing 3 ~ 5 times, be placed on and constantly pass in the saturated calcium sulphate soln of oxygen, soak 3 ~ 5h;
(2) treat that seed is in the saturated calcium sulphate soln passing into oxygen, when imbibition to volume is 1.5 times of original volume, to be taken out and the diameter be placed in distilled water is wetting is on 7cm filter paper, often open filter paper and place 3 ~ 5 seeds, the filter paper that placed seed is placed in dark vernalization, when seed shows money or valuables one carries unintentionally, be placed in the quartz sand matrix of sterilizing at 170 ~ 190 DEG C, every 3 days to pressing the amount of 3 strain 10mL to its quantitative watering, until its growth final singling after 10 ~ 15 days, every basin stays seedling 10 strain;
(3) count by weight, choose the nitrate of lime of the deionized water of 35 ~ 65 parts, the potassium nitrate of 15 ~ 25 parts, the magnesium sulfate of 5 ~ 15 parts and 15 ~ 25 parts, it is mixed in order it to dissolve completely, configure to obtain plant nutrition liquid, by every basin 100 ~ 150mL plant nutrition liquid, every day, 12 noon was to its fertilizer treatment, was cultured to the colored pod phase;
(4) treat that soybean soybean plant strain is cultured to colored pod after date, after cultivating 2 ~ 3h under being placed on sunlight in morning, with deionized water washing plant root and the quartz sand of 3 ~ 5L, remove the nutrient ion of the quartz sand absorption of root system of plant and non-root exudates, subsequently, by its illumination cultivation 5 ~ 6h under original condition;
(5) after having cultivated under original condition, with 2L deionized water drip washing plant root, and collect leacheate in beaker, and be 10:1 by leacheate and microbial inhibitor mass ratio, add isothiazolinone in above-mentioned leacheate, and at being placed on 40 ~ 45 DEG C, 1/2 of the most original volume of rotary evaporation being vacuumized to it and can be prepared into a kind of soybean nodulation germ inhibitor.
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| CN201510636730.5A CN105340412A (en) | 2015-10-01 | 2015-10-01 | Preparation method for soybean nodulation pathogenic-bacterium inhibitor |
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| CN201510636730.5A CN105340412A (en) | 2015-10-01 | 2015-10-01 | Preparation method for soybean nodulation pathogenic-bacterium inhibitor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717213A (en) * | 2019-01-28 | 2019-05-07 | 广西大学 | A kind of disease-resistant tomato stock root exudates collection liquid and its suppressing method to ralstonia solanacearum |
| CN109717212A (en) * | 2019-01-28 | 2019-05-07 | 广西大学 | A kind of method that disease-resistant stock root exudates is collected and extracted |
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| US5628144A (en) * | 1987-04-03 | 1997-05-13 | Kamterter Products, Inc. | Solid matrix priming of seeds with microorganisms and selected chemical treatment |
| CN1951197A (en) * | 2006-11-01 | 2007-04-25 | 沈阳农业大学 | A novel method for controlling soybean root disease by using rhizobia |
| CN101935238A (en) * | 2009-07-02 | 2011-01-05 | 甘肃农业大学 | Pollution-resistance nitragin and preparation method thereof |
| CN104351047A (en) * | 2014-08-28 | 2015-02-18 | 中国农业科学院农业资源与农业区划研究所 | Preparation method of soybean root exudate for efficiently promoting competitive nodulation of rhizobium japonicum |
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2015
- 2015-10-01 CN CN201510636730.5A patent/CN105340412A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5628144A (en) * | 1987-04-03 | 1997-05-13 | Kamterter Products, Inc. | Solid matrix priming of seeds with microorganisms and selected chemical treatment |
| CN1951197A (en) * | 2006-11-01 | 2007-04-25 | 沈阳农业大学 | A novel method for controlling soybean root disease by using rhizobia |
| CN101935238A (en) * | 2009-07-02 | 2011-01-05 | 甘肃农业大学 | Pollution-resistance nitragin and preparation method thereof |
| CN104351047A (en) * | 2014-08-28 | 2015-02-18 | 中国农业科学院农业资源与农业区划研究所 | Preparation method of soybean root exudate for efficiently promoting competitive nodulation of rhizobium japonicum |
Non-Patent Citations (3)
| Title |
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| 张俊英 等: "大豆根系分泌物中氨基酸对根腐病菌生长的影响", 《植物营养与肥料学报》 * |
| 王媛媛 等: "根瘤内生细菌对大豆胞囊线虫及根腐病菌的影响", 《大豆科学》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717213A (en) * | 2019-01-28 | 2019-05-07 | 广西大学 | A kind of disease-resistant tomato stock root exudates collection liquid and its suppressing method to ralstonia solanacearum |
| CN109717212A (en) * | 2019-01-28 | 2019-05-07 | 广西大学 | A kind of method that disease-resistant stock root exudates is collected and extracted |
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Application publication date: 20160224 |