CN105311177A - Pharmaceutical composition having gastric mucosa protection effect, preparation method and applications thereof - Google Patents
Pharmaceutical composition having gastric mucosa protection effect, preparation method and applications thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of traditional Chinese medicine preparations and health food, and particularly relates to a pharmaceutical composition with effects of gastric mucosa protection, chronic gastritis prevention and treatment, and stomach ulcer prevention and treatment, and a preparation method thereof, and applications of the pharmaceutical composition in drugs and health food. According to the present invention, the drug or health food adopts grifola frondosa as the main active component, citrus medica l. var. sarcodactylis swingle, atractylodes macrocephala and cynanchum otophyllum are combined, and the further matching is performed; the drug or health food can promote gastric mucosa epithelial cell proliferation, promote gastric mucosa epithelial cell to migrate to the wound surface, and accelerate damaged area epithelial cell growth so as to promote gastric mucosa injury repair; and the pharmaceutical composition can be used for preparation of drugs and health food with effects of gastric mucosa protection, chronic gastritis prevention and treatment, and stomach ulcer prevention and treatment, and has good application prospects and good economical benefits.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicine preparation and technical field of health care food, be specifically related to a kind of to there is protection gastric mucosa, prevent and treat chronic gastritis, the pharmaceutical composition of gastric ulcer, preparation method and the application in medicine, health food thereof.
Background technology
Chronic gastritis (chronicgastritis) is a kind of commonly encountered diseases, frequently-occurring disease, and its diseased region various places all over the world, relate to all kinds of crowd.And along with progress of modern society, rhythm of life is accelerated, and various pressure is ubiquitous especially, and various inherent extrinsic factor all constantly stimulate, destroy the gastric mucosa of people, cause many diseases such as pain, inflammation that have a stomach-ache, chronic gastritis is become affects a large disease of human health.Chronic gastritis is divided into constitutional and Secondary cases two class, and primary gastritis is divided into again shallow, atrophic and hypertrophica 3 kinds.
Research display, the cause of disease of the diseases such as chronic gastritis comprise unsound life style as stayed up late, tired, drink, drink the reasons such as strong tea, also comprise helicobacter pylori infections, medicine factor (as aspirin etc.), also have Nervous and Mental Factors and inherited genetic factors etc.The pathological change of chronic gastritis, is confined to mucous layer substantially, and major embodiment gastric mucosa is impaired, therefore strictly says and should be referred to as " chronic gastric mucositis " or " gastric mucosa is sick ".Therefore, study hotspot and pipe fitting that gastric mucosa becomes Prevention and Curation chronic gastric inflammation how is protected.
At present, the treatment for chronic gastritis there is no specific short, generally advocates that asymptomatic person is without the need to treating, if there is symptom can treat with reference to following method:
1, daily health caring avoids the factor causing acute gastritis, avoids taking to stomach food excitatory and medicine as NSAID etc. as given up tobacco and wine;
2, Diet Therapy principle is similar to Peptic Ulcers, repeatedly eats less, and soft diet is main, avoids raw food and irritable food, the more important thing is the dietary habit according to patient and many years of experience, sums up a set of recipe being applicable to oneself;
3, Drug therapy Hp Gastritis need carry out eradicating the treatment of Hp, and other chronic gastritiss there is no specific short, and gastritis mostly can not be made to reverse, therefore mainly symptomatic treatment.The most gastric acid of chronic gastritis is on the low side or anacidity, but itself is without special treatment, can give dilute hydrochloric acid and pepsin in right amount.As 1% hydrochloric acid l0ml, oral, 3 times/d, Pepsin Mixture 10ml, oral, 3 times/d.But gastric secretion reaches 1.5-2.5L every day, 30ml1% hydrochloric acid can not change the pH of gastric juice at all.Also some patient is counter after taking hydrochloric acid feels stomach discomfort, may be mucosal erosion, caused by hydrochloric acid stimulates.Have report cimetidine (cimetidine) to treat chronic gastritis, curative effect is also comparatively remarkable, and Swynnerton treats also to the dyspepsia clothes for patients alkalescent medicine of anacidity, also obtains good effect.These there is no method interpretation theoretically, but true experience is like this.
Visible, at present for the treatment of chronic gastritis still based on the Western medicine that zest is stronger, this also may cause the wound of secondary for gastric mucosa in damaged condition itself.All the time, Chinese medicine is all very popular with the therapeutic modality of its gentleness, therefore, also becomes the focus and direction studied with treatment by Chinese herbs chronic gastritis a few days ago.
Summary of the invention
For this reason, the object of the present invention is to provide one to have protection gastric mucosa effect, effectively can treat chronic gastritis, the pharmaceutical composition of gastric ulcer and health food, and openly its preparation method further.
For solving the problems of the technologies described above, the invention provides a kind of pharmaceutical composition with the effect of protection gastric mucosa, it is characterized in that, the crude drug of described pharmaceutical composition consists of: Effects of Extracts of Grifola frondosa on Active 1-10 weight portion; Rhizoma Atractylodis Macrocephalae 1-10 weight portion; Radix Paeoniae Alba 1-6 weight portion; Fructus Citri Sarcodactylis 1-6 weight portion.
Further, the crude drug of described pharmaceutical composition consists of: Effects of Extracts of Grifola frondosa on Active 5 weight portion; The Rhizoma Atractylodis Macrocephalae 5 weight portion; The Radix Paeoniae Alba 3 weight portion; Fructus Citri Sarcodactylis 3 weight portion.
Described pharmaceutical composition adds customary adjuvant, and be conventionally prepared into clinical acceptable dosage form, described dosage form includes but not limited to capsule, tablet, electuary, oral liquid, granule, powder, pill, solid beverage.
The invention also discloses and add customary adjuvant by described pharmaceutical composition, the preparation be conventionally prepared into.
The invention also discloses the method preparing described pharmaceutical composition, comprise and measure Effects of Extracts of Grifola frondosa on Active, the Rhizoma Atractylodis Macrocephalae, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis according to selected prescription, and the step of mix homogeneously, add customary adjuvant, conveniently technique and make acceptable dosage form clinically.
Further, described method is: get the Rhizoma Atractylodis Macrocephalae of recipe quantity, the Radix Paeoniae Alba, Fructus Citri Sarcodactylis mixing, add 6-10 times of times of weight water extraction 1-3 time, each 0.5-2 hour, propose merging and get liquid, relative density 1.10-1.20g/mL during vacuum concentration to 90 DEG C, obtains condensed cream; Effects of Extracts of Grifola frondosa on Active and the described condensed cream of getting recipe quantity mix, and add customary adjuvant, are conventionally prepared into clinical acceptable capsule, tablet, electuary, oral liquid, granule, powder, pill, solid beverage.
Further, adopt common process prepare needs such as capsule, tablet and granule carry out drying dosage form time, employing is carried out belt vacuum drying mode and is carried out drying, its charging rate 3000-5000mL/h of preferred control, every section of temperature be respectively 90-100 DEG C, 90-100 DEG C, 85-95 DEG C, 70-80 DEG C, 20-30 DEG C, belt speed 50-200mm/min, band dry material is after granulate, add 30-70% ethanol wet granulation, after 50-80 DEG C of oven dry, then carry out follow-up granulate and/or tabletting program.
Described method also comprises step Grifola frondosa conventionally extracted and prepare described Effects of Extracts of Grifola frondosa on Active.
Further, described Effects of Extracts of Grifola frondosa on Active extracts in accordance with the following steps: get Grifola frondosa 10-20 weight portion, section, add 10-20 times of weight water extraction 1-3 time, each 0.5-2 hour, merge extractive liquid, during vacuum concentration to 60 DEG C, relative density is 1.10-1.30g/mL, obtains described Effects of Extracts of Grifola frondosa on Active.
The invention also discloses compositions that described compositions or described method prepare and there is in preparation purposes in the medicine of protection gastric mucosa effect and health food.
The invention also discloses the purposes of compositions in the medicine and health food of preparation treatment chronic gastritis, gastric ulcer that described compositions or described method prepare.
Chinese medicine composition of the present invention is used as medicine by Effects of Extracts of Grifola frondosa on Active, the Rhizoma Atractylodis Macrocephalae, the Radix Paeoniae Alba, Fructus Citri Sarcodactylis, and the effective ingredient grifolan in Effects of Extracts of Grifola frondosa on Active proves to have the effect promoting people's gastric epithelial cells propagation, thus promotes the reparation of mucosal lesion; Rhizoma Atractylodis Macrocephalae invigorating the spleen and benefiting QI, dampness diuretic, strengthening superficial resistance to stop perspiration; The Radix Paeoniae Alba is enriched blood easing the affected liver, suppressing the hyperactive liver pain relieving.Yin fluid astringing receives antiperspirant; Fructus Citri Sarcodactylis depressed liver-energy dispersing and QI regulating, and stomach and alleviating pain, preventing phlegm from forming and stopping coughing.We, based on Effects of Extracts of Grifola frondosa on Active, coordinate the Chinese herbal medicine of invigorating the spleen and benefiting QI nourishing the stomach, have good reparation and protective effect to mucosal lesion.
Experiment shows, Chinese medicine composition of the present invention has the effect promoting people's gastric epithelial cells propagation, and for repair in trauma experiment, protection gastric mucosa granule can promote that GES-1 moves to wound face,
Accelerate the epithelial growth of damage field, thus promote the reparation of mucosal lesion, effective protective effect can be played to gastric mucosa, to the treatment of gastric ulcer, there are positive functions and effects.Therefore the medicine of the present invention to chronic disease such as preparation treatment gastric ulcer etc. opens a kind of new treatment by Chinese herbs method, and not only effective percentage is high and side effect is low, and processing technology is simple, and high financial profit, has good application prospect.
Experimental example
1. testing program
1.1 test materials and equipment
People's gastric epithelial cell (GES-1) is purchased from Tumour Inst., Chinese Medical Academy;
DMEM high glucose medium, hyclone, 0.2% trypsin are all purchased from GIBCO;
MTS (Promega); The pharmaceutical composition (by the made granule of embodiment 1) of protection gastric mucosa;
Biohazard Safety Equipment (HealForce power health); Digital difference inverted microscope (IX51Olympus); CO2 gas incubator (esco); High speed refrigerated centrifuge (5810REppendorf); Microplate reader (sunrisetecan).
1.2 test method
1.2.1 polysaccharide solution preparation
Cell culture fluid: DEME high glucose medium, 10% hyclone, 100UmL
-1penicillin, streptomycin; Protection gastric mucosa granule is dissolved in cell culture fluid, is mixed with 0 ~ 50mgmL
-1variable concentrations, 0.22 μm of filtering with microporous membrane is degerming, and 4 DEG C of refrigerators keep in Dark Place for subsequent use.
1.2.2 cell culture
People's gastric epithelial cell GES-1, put 37 DEG C, saturated humidity, 5%CO
2aseptic culture case is cultivated, and every 2 ~ 3d changes a not good liquor, and every 3 ~ 4d goes down to posterity, and makes cell concentration maintain 70% ~ 80% degrees of fusion.1.2.3 the impact that gastric mucosa granule is bred people's gastric epithelial cell GES-1 is protected
To take the logarithm trophophase cell, its concentration is adjusted to 2.0 × 104 mL
-1, be inoculated in 96 orifice plates, every hole 100 μ L, cultivate after 24h and change culture fluid, experimental group adds 50 respectively, 10,2,0.4,0.08mgmL
-1the each 200 μ L of protection gastric mucosa particle solution; blank group is cell culture fluid; solvent blank group is corresponding protection gastric mucosa particle solution, and often group establishes 6 repetitions, cultivate 1,2,3, after 4d; add MTS solution 20 μ L; continue to cultivate 4h, use microplate reader to survey the absorbance of 492nm wavelength, calculate cell proliferation rate; experiment in triplicate, and records its growth conditions.
1.2.4 the impact that gastric mucosa granule moves people's gastric epithelial cell GES-1 is protected
The foundation of gastric mucosa trauma model: people's gastric epithelial cells of trophophase of taking the logarithm, is inoculated in 24 orifice plates with 6 × 105, every hole cell, and every hole 400 μ L, puts 37 DEG C, 5%CO
2cultivate 24h in incubator, after iuntercellular is formed and connects into monolayer more closely, with diameter be the capillary tube of 1.5mm at the central vertical of culture dish standardized road vestige, and wound surface both sides can be seen under the microscope simultaneously.Sucking-off original fluid after scratching; every hole 200 μ LPBS wash twice with the non-attached cell of Ex-all; then each hole adds the culture fluid 400 μ L of different protection gastric mucosa granule final concentration; matched group only adds DMEM culture fluid 400 μ L; application specialized image analysis software Imageproplus6.0 measures 0 respectively; the cut wound healing situation of 6,12,24h.
1.3 date processing
Experimental data with average ± standard deviation (
) represent, use SPSS13.0 software processes, adopt method of analysis of variance, it is P<0.05 that difference has significance level, and pole significance level is P<0.01.
2 results and analysis
2.1 protection gastric mucosa granules are on the impact of GES-1 cell proliferation
Concentration, the action time of protection gastric mucosa granule promotion people's gastric epithelial cells GES-1 cultivation effect and protection gastric mucosa granule have important relation.In the effect incubation time of 96h, protection gastric mucosa pellet design concentration is 0.08mgmL
-1, 0.4mgmL
-1dosage group has short cultivation effect to people's gastric epithelial cell GES-1, wherein 0.08mgmL
-1, 0.4mgmL
-1group is urged the rate of increase at 48h and is reached 398.77%, 382.88%, and difference reaches extremely significantly (P<0.01).
According to proliferation experiment result, select 0.4mgmL
-1protection gastric mucosa granule density group is carried out effect again and is cultivated, and uses inverted microscope to observe GES-1 cell growth condition and form thereof.The GES-1 cell that protection gastric mucosa groups of grains is cultivated is obviously better than CK growth conditions; 24hCK group cell growth state is compared obviously poor; cellular morphology is little; circular; have no adherent; 48h protects gastric mucosa groups of grains adherent growth obvious; increasing number; cell is normal flat polygon or is bordering on fusiformis; have pseudopodium, although the nutritional labeling protecting gastric mucosa groups of grains quantity to increase to some extent due to culture fluid after 72h is used up, cell state starts to occur change; to 96h, cell starts to occur that atrophy becomes circle.
Can obtain from above-mentioned 2 experimental results, certain density protection gastric mucosa granule has the effect promoting people's gastric epithelial cells propagation.
2.2 protection gastric mucosa granules are on the impact of GES-1 cell migration
Select short GES-1 cell proliferation effect protect preferably gastric mucosa granule density group (0.4,0.08mgmL
-1) carrying out repair in trauma experiment, 2 kinds of concentration protection gastric mucosa granules all obviously promote the migration of GES-1 cell.Effect is cultivated 6h and is just presented remarkable result (P<0.05); When cultivating 24h, 0.4mgmL
-1the mobility that protection gastric mucosa granule urgees GES-1 cell reaches 96.69%, and reach significant effect (P<0.01), matched group is only 53.4%.
Due to the function of stomach and the special of residing internal medium thereof, gastric mucosa is tissue more easily damaged in humans and animals body, though gastric mucosa easy damaged, it repairs fast, it is one of tissue that in body, own metabolism is the fastest, and the average 2 ~ 3d of its epithelial cell changes once.Pass through microscopic examination; gastric epithelial cells has very strong self-regeneration function; cell can occur to move and make it healing near cut wound surface; after adding protection gastric mucosa granule in culture fluid, migration velocity is faster; 24h can reach 96.69%; visible protection gastric mucosa granule obviously can promote the migration of gastric epithelial cell, accelerates the healing of cut wound surface.
3 discuss and conclusion
Gastric mucosa ulcer occur and healing process in play an important role.The reparation of mucosa ulcer generally comprises following two processes: first, and the reparation of mucosa is a cell reconstitution process fast, mainly comprises impaired mucosa cells and comes off, by adjoin or the bottom cells migrate of damaged part, cover impaired mucous membrane surface; Secondly, the cell that affected area is lost is replaced by the cell of hypertrophy and is supplemented, and mucosa is returned to normal thickness and structure.
This experiment shows; protection gastric mucosa granule has the effect promoting people's gastric epithelial cells propagation; repair in trauma is tested; protection gastric mucosa granule can promote that GES-1 moves to wound face; accelerate the epithelial growth of damage field; thus promote the reparation of mucosal lesion, think the probability of application in chronic gastritis, gastric ulcer treatment thus.
Detailed description of the invention
Embodiment 1 granule
[prescription] Effects of Extracts of Grifola frondosa on Active 5kg, Rhizoma Atractylodis Macrocephalae 5kg, Radix Paeoniae Alba 3kg, Fructus Citri Sarcodactylis 3kg.
[preparation method] gets the Rhizoma Atractylodis Macrocephalae of recipe quantity, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis mixing, and add 8 times of weight purified water extraction 2 times, each 1 hour, propose merging and get liquid, vacuum concentration, to relative density 1.20g/mL (90 DEG C of surveys), obtains condensed cream; Get recipe quantity Effects of Extracts of Grifola frondosa on Active fully to mix with condensed cream and customary adjuvant, carry out belt vacuum drying (charging rate 3000mL/h, every section of temperature 95 DEG C, 95 DEG C, 90 DEG C, 75 DEG C, temperature 20 DEG C, belt speed 100mm/min), band dry material, after granulate, adds 40% ethanol wet granulation, 60 DEG C of oven dry, granulate, subpackage, to obtain final product.
Embodiment 2 capsule
[prescription] Effects of Extracts of Grifola frondosa on Active 3 weight portion, the Rhizoma Atractylodis Macrocephalae 8 weight portion, the Radix Paeoniae Alba 2 weight portion, Fructus Citri Sarcodactylis 4 weight portion.
[preparation method] gets the Rhizoma Atractylodis Macrocephalae of recipe quantity, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis mixing, and add 6 times of weight purified water extraction 2 times, each 0.5 hour, propose merging and get liquid, vacuum concentration, to relative density 1.15g/mL (90 DEG C of surveys), obtains condensed cream; The Effects of Extracts of Grifola frondosa on Active of condensed cream and recipe quantity, customary adjuvant are fully mixed, carries out belt vacuum drying (charging rate 3500mL/h, every section of temperature 98 DEG C, 95 DEG C, 85 DEG C, 70 DEG C, temperature 25 DEG C, belt speed 100mm/min), band dry material is after granulate, add 60% ethanol wet granulation, 70 DEG C of oven dry, granulate, filled capsules, aluminum-plastic packaged or bottling, to obtain final product.
Embodiment 3 tablet
[prescription] Effects of Extracts of Grifola frondosa on Active 7 weight portion, the Rhizoma Atractylodis Macrocephalae 4 weight portion, the Radix Paeoniae Alba 6 weight portion, Fructus Citri Sarcodactylis 2 weight portion.
[preparation method] gets the Rhizoma Atractylodis Macrocephalae of recipe quantity, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis mixing, and add 10 times of weight purified water extraction 2 times, each 2 hours, propose merging and get liquid, vacuum concentration, to relative density 1.10g/mL (90 DEG C of surveys), obtains condensed cream; Condensed cream is fully mixed with Effects of Extracts of Grifola frondosa on Active, customary adjuvant, carries out belt vacuum drying (charging rate 3000mL/h, every section of temperature 99 DEG C, 90 DEG C, 85 DEG C, 70 DEG C, temperature 30 DEG C, belt speed 150mm/min), band dry material is after granulate, add 50% ethanol wet granulation, 80 DEG C of oven dry, granulate, tabletting, aluminum-plastic packaged or bottling, to obtain final product.
Embodiment 4 oral liquid
[prescription] Effects of Extracts of Grifola frondosa on Active 6 weight portion, the Rhizoma Atractylodis Macrocephalae 10 weight portion, the Radix Paeoniae Alba 2 weight portion, Fructus Citri Sarcodactylis 5 weight portion.
[preparation method] gets the Rhizoma Atractylodis Macrocephalae of recipe quantity, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis mixing, and add 6 times of weight purified water extraction 3 times, each 0.5 hour, propose merging and get liquid, vacuum concentration, to relative density 1.20g/mL (90 DEG C of surveys), obtains condensed cream; Mixed homogeneously with Effects of Extracts of Grifola frondosa on Active, customary adjuvant and correctives by condensed cream, standardize solution, sterilizing, fill, to obtain final product.
Embodiment 5 solid beverage
[prescription] Effects of Extracts of Grifola frondosa on Active 10 weight portion, the Rhizoma Atractylodis Macrocephalae 5 weight portion, the Radix Paeoniae Alba 4 weight portion, Fructus Citri Sarcodactylis 4 weight portion.
[preparation method] gets the Rhizoma Atractylodis Macrocephalae of recipe quantity, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis mixing, and add 8 times of weight purified water extraction 2 times, each 2 hours, propose merging and get liquid, vacuum concentration, to relative density 1.15g/mL (90 DEG C of surveys), obtains condensed cream; Condensed cream is fully mixed with Effects of Extracts of Grifola frondosa on Active, customary adjuvant, carries out spraying dry, obtain extract powder, subpackage, to obtain final product.
Embodiment 6 granule
[prescription] Effects of Extracts of Grifola frondosa on Active 5 weight portion, the Rhizoma Atractylodis Macrocephalae 5 weight portion, the Radix Paeoniae Alba 3 weight portion, Fructus Citri Sarcodactylis 3 weight portion.
[preparation method] gets the Rhizoma Atractylodis Macrocephalae of recipe quantity, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis mixing, and add 8 times of weight purified water extraction 2 times, each 1 hour, propose merging and get liquid, vacuum concentration, to relative density 1.20g/mL (90 DEG C of surveys), obtains condensed cream; Condensed cream is fully mixed with Effects of Extracts of Grifola frondosa on Active, customary adjuvant, carries out belt vacuum drying (charging rate 3000mL/h, every section of temperature 95 DEG C, 95 DEG C, 90 DEG C, 75 DEG C, temperature 20 DEG C, belt speed 100mm/min), band dry material, after granulate, adds 40% ethanol wet granulation, 60 DEG C of oven dry, granulate, subpackage, to obtain final product.
Embodiment 7 capsule
[prescription] Effects of Extracts of Grifola frondosa on Active 1 weight portion, the Rhizoma Atractylodis Macrocephalae 10 weight portion, the Radix Paeoniae Alba 1 weight portion, Fructus Citri Sarcodactylis 6 weight portion.
[preparation method] gets the Rhizoma Atractylodis Macrocephalae of recipe quantity, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis mixing, and add 6 times of weight purified water extraction 2 times, each 0.5 hour, propose merging and get liquid, vacuum concentration, to relative density 1.15g/mL (90 DEG C of surveys), obtains condensed cream; Condensed cream is fully mixed with Effects of Extracts of Grifola frondosa on Active, customary adjuvant, carries out belt vacuum drying (charging rate 3500mL/h, every section of temperature 98 DEG C, 95 DEG C, 85 DEG C, 70 DEG C, temperature 25 DEG C, belt speed 100mm/min), band dry material is after granulate, add 60% ethanol wet granulation, 70 DEG C of oven dry, granulate, filled capsules, aluminum-plastic packaged or bottling, to obtain final product.
Embodiment 8 tablet
[prescription] Effects of Extracts of Grifola frondosa on Active 6 weight portion, the Rhizoma Atractylodis Macrocephalae 1 weight portion, the Radix Paeoniae Alba 6 weight portion, Fructus Citri Sarcodactylis 1 weight portion.
[preparation method] gets the Rhizoma Atractylodis Macrocephalae of recipe quantity, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis mixing, and add 10 times of weight purified water extraction 2 times, each 2 hours, propose merging and get liquid, vacuum concentration, to relative density 1.10g/mL (90 DEG C of surveys), obtains condensed cream; Condensed cream is fully mixed with Effects of Extracts of Grifola frondosa on Active, customary adjuvant, carries out belt vacuum drying (charging rate 3000mL/h, every section of temperature 99 DEG C, 90 DEG C, 85 DEG C, 70 DEG C, temperature 30 DEG C, belt speed 150mm/min), band dry material is after granulate, add 50% ethanol wet granulation, 80 DEG C of oven dry, granulate, tabletting, aluminum-plastic packaged or bottling, to obtain final product.
Obviously, above-described embodiment and experimental example are only for clearly example being described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of extending out or variation be still among the protection domain of the invention.
Claims (10)
1. have a pharmaceutical composition for protection gastric mucosa effect, it is characterized in that, the crude drug of described pharmaceutical composition consists of: Effects of Extracts of Grifola frondosa on Active 1-10 weight portion; Rhizoma Atractylodis Macrocephalae 1-10 weight portion; Radix Paeoniae Alba 1-6 weight portion; Fructus Citri Sarcodactylis 1-6 weight portion.
2. pharmaceutical composition as claimed in claim 1, it is characterized in that, the crude drug of described pharmaceutical composition consists of: Effects of Extracts of Grifola frondosa on Active 5 weight portion; The Rhizoma Atractylodis Macrocephalae 5 weight portion; The Radix Paeoniae Alba 3 weight portion; Fructus Citri Sarcodactylis 3 weight portion.
3. pharmaceutical composition as claimed in claim 1 or 2, is characterized in that, described Chinese medicine composition adds customary adjuvant, is conventionally prepared into clinical acceptable capsule, tablet, electuary, oral liquid, granule, powder, pill, solid beverage.
4. add customary adjuvant, the preparation be conventionally prepared into by the arbitrary described pharmaceutical composition of claim 1-3.
5. prepare the method for the arbitrary described pharmaceutical composition of claim 1-3 for one kind, it is characterized in that, comprise and measure Effects of Extracts of Grifola frondosa on Active, the Rhizoma Atractylodis Macrocephalae, the Radix Paeoniae Alba and Fructus Citri Sarcodactylis according to selected prescription, and the step of mix homogeneously, add customary adjuvant, conveniently technique and make acceptable dosage form clinically.
6. preparation method as claimed in claim 5, it is characterized in that, the method is: get the Rhizoma Atractylodis Macrocephalae of recipe quantity amount, the Radix Paeoniae Alba, Fructus Citri Sarcodactylis mixing, add 6-10 times of times of weight water extraction 1-3 time, each 0.5-2 hour, propose merging and get liquid, relative density 1.10-1.20g/mL during vacuum concentration to 90 DEG C, obtains condensed cream; Effects of Extracts of Grifola frondosa on Active and the described condensed cream of getting recipe quantity mix, and add customary adjuvant, are conventionally prepared into clinical acceptable capsule, tablet, electuary, oral liquid, granule, powder, pill, solid beverage.
7. the preparation method as described in claim 5 or 6, is characterized in that, the method also comprises step Grifola frondosa conventionally extracted and prepare Effects of Extracts of Grifola frondosa on Active.
8. preparation method according to claim 7, it is characterized in that, described Effects of Extracts of Grifola frondosa on Active extracts in accordance with the following steps: get Grifola frondosa 10-20 weight portion, section, add 10-20 times of weight water extraction 1-3 time, each 0.5-2 hour, merge extractive liquid, during vacuum concentration to 60 DEG C, relative density is 1.10-1.30g/mL, obtains described Effects of Extracts of Grifola frondosa on Active.
9. the pharmaceutical composition that the arbitrary described pharmaceutical composition of claim 1-3 or the arbitrary described method of claim 5-8 prepare has the purposes in the medicine and health food protecting gastric mucosa effect in preparation.
10. the pharmaceutical composition for preparing of the arbitrary described pharmaceutical composition of claim 1-3 or the arbitrary described method of claim 5-8 prevents and treats the purposes in chronic gastritis, the medicine of gastric ulcer and health food in preparation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112516129A (en) * | 2021-01-09 | 2021-03-19 | 兰州大学 | Application of trans-1, 3-dilinolein in preparation of drugs for treating gastric cancer, drugs for invigorating stomach, health products and foods |
| CN114107172A (en) * | 2021-11-12 | 2022-03-01 | 上海市农业科学院 | Method for constructing non-infectious gastric mucosa injury cell model and application |
-
2014
- 2014-07-28 CN CN201410362469.XA patent/CN105311177A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 白鸿: "《保健食品功效成分检测方法》", 31 May 2011, 中国中医药出版社 * |
| 陈朝青等: "灰树花提取物对大鼠胃黏膜的保护作用", 《中国美容医学》 * |
| 陈长青等: ""白术-白芍"药对不同提取工艺对药效作用的影响", 《中药新药与临床药理》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112516129A (en) * | 2021-01-09 | 2021-03-19 | 兰州大学 | Application of trans-1, 3-dilinolein in preparation of drugs for treating gastric cancer, drugs for invigorating stomach, health products and foods |
| CN112516129B (en) * | 2021-01-09 | 2022-04-29 | 兰州大学 | Application of trans-1, 3-dilinolein in preparation of drugs for treating gastric cancer, drugs for invigorating stomach, health products and foods |
| CN114107172A (en) * | 2021-11-12 | 2022-03-01 | 上海市农业科学院 | Method for constructing non-infectious gastric mucosa injury cell model and application |
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Application publication date: 20160210 |