CN105309287A - Soilless culture method for horseradish - Google Patents
Soilless culture method for horseradish Download PDFInfo
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- CN105309287A CN105309287A CN201410263567.8A CN201410263567A CN105309287A CN 105309287 A CN105309287 A CN 105309287A CN 201410263567 A CN201410263567 A CN 201410263567A CN 105309287 A CN105309287 A CN 105309287A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention relates to the technical field of purpose-made plant plating, in particular to a soilless culture method for horseradish. The soilless culture method includes the steps of culture device installing, horseradish seedling culturing, seedling hardening, transplanting and managing. Indoor culture pipes are used for planting horseradish, soilless culture of horseradish is achieved, horseradish is cultured in a gloomy and cold room, the natural growth condition of horseradish is simulated well, and therefore the most suitable growth environment is provided for growth of horseradish; as seedling hardening is carried out before transplanting, tolerance of horseradish to unsuitable environments is greatly improved, and the survival rate of horseradish is effectively increased; pest and disease damage to horseradish is reduced by means of indoor culture, labor amount for horseradish culture is reduced, and production cost of horseradish is effectively reduced.
Description
Technical field
The present invention relates to special planting technical field, specifically the soilless culture method of a kind of horseradish.
Background technology
The nutriment that soilless cultivation plant utilizes is the various fertilizer manually allocated in nutrient solution, have without matrix, only just passable by water planting, some matrix, but matrix is not for plant provides any nutrient, this planting patterns cost compare is high, but because optimum nutrient condition can be provided for plant, so output is very high, but soilless culture is to capital, equipment, the requirement of technology etc. is all higher, so only have the vegetable melon and fruit of minority and this planting patterns kind of rare flower at present out.
Horseradish has another name called scurvy grass in China's Mainland, formal name used at school Wasabia (transliteration " Wa Shabi "), is Cruciferae happiness my year negative raw herbaceous plant, is described as " green gold " by people.In Japan, horseradish has the cultivation history in more than 350 years, just just walks its plantation in China.Horseradish be used as tune product be mainly manifested in perfume (or spice), pungent, peppery, rush four characteristics, be the indispensable condiment of Japanese cuisine.It contains the various trace elements of abundant amino acid, vitamin and needed by human body, has to improve a poor appetite, antisepsis and sterilization, establishing-Yang, to purify the blood and analgesic activity, has very high edible and medical value.
Because the growth of horseradish requires high to ecotope, need are sombre, moist, ventilative, water quality is good, and therefore the place of suitable planting is few, according to interrelated data introduction, domestic only have Lagotis yunnanesis at present, and the plantation of certain scale nonirrigated farmland is carried out in the mountain and forest region of the Baoshan and Erlangshan, Sichuan Province.The restriction of natural conditions, greatly have impact on the development of horseradish industry.
Summary of the invention
The object of the invention is, for above-mentioned Problems existing, to provide the soilless culture method of a kind of horseradish.
For achieving the above object, the technical solution used in the present invention is: the soilless culture method of a kind of horseradish, comprises the steps:
(1) installation of culturing device
In the culturing room of cool, damp, being 16 ~ 20cm by diameter is arranged on the high support of overhead 20 ~ 30cm with the cultivation tube side-by-side of field planting hole, then tube connector is utilized to be connected by cultivation pipe head and the tail, be provided with infusion pump in the front end of first cultivation pipe, finally with tube connector, cultivation pipe, liquid storage tank, infusion pump be connected to form the hydroponic system that a culture fluid can circulate;
(2) horseradish plantlet is cultivated
A
1select the horseradish kind needing to carry out cultivating, seed is kept 18 ~ 22 DEG C, and humidity is 90%, and short its germinates;
A
2when wasabi seed germination length is 1 ~ 2cm, cut stem apex, length is 0.3 ~ 0.4cm;
A
3by steps A
2after the bud cleaning put in order, with gauze parcel, utilize running water continual rinsing 2 hours, then soak 30min, finally with aseptic this suck dry moisture of moisture absorption paper substrate with the carbendazim solution of 1% concentration;
A
4will through steps A
3obtained horseradish bud stem apex utilizes alcohol-pickled 28 ~ 32 seconds of 70% volumetric concentration, then utilizes 0.2%HgCl
2carry out surface sterilization, finally use sterile water wash 5 times;
A
5medium is utilized to cultivate into aseptic horseradish plantlet the stem apex after sterilizing;
A
6the shoot apical meristem of educating through being commissioned to train at the beginning of sterilizing detoxification is transferred in the 1/2MS medium not adding hormone and grows;
(3) hardening
Utilize above-mentioned steps A
6medium continue, the amount of medium was removed the half to only having initial medium amount every 2 ~ 3 days by period, cultivated 1 ~ 2 day, was then filling it up with medium, so circulating, and cultivated and completed hardening after 20 ~ 27 days;
(4) transplant
First will in culture apparatus, utilize infusion pump to pump into horseradish special culture solution, allow its culture fluid be full of cultivation pipe; Take out the horseradish seedling after hardening, utilize running water to wash away the medium in horseradish shoot root portion, the root then utilizing sponge to wrap horseradish seedling is put into culture hole and is cultivated, and the transfusion speed simultaneously controlling infusion pump avoids culture fluid flow velocity to rush askew by horseradish seedling soon;
(5) manage
After horseradish seedling survives, changed a horseradish special culture solution every 15 ~ 30 days, then joint carries out the process of turk platform, during turk platform during the blossom season, base portion stays 3 ~ 5cm to protect stem, and control cultivation indoor temperature 18 ~ 23 DEG C, humidity 60 ~ 80%, intensity of illumination are 2000 ~ 2400Lux.
Further, in step (1), the distance between two adjacent described field planting holes is 30 ~ 40cm.
Further, steps A
6in, be added with the root-growing agent that concentration is 0.3 ~ 0.5mg/L in described 1/2MS medium, root-growing agent is one in IBA, NAA, IAA or its mixture.
Further, in step (5), described horseradish special culture solution is the aqueous solution containing horseradish special fertilizer, and the part by weight of special fertilizer and water is 1 ~ 2:50.
Advantageous Effects of the present invention is:
(1) the present invention adopts indoor growing pipe plantation horseradish, achieve the soilless culture of horseradish, in sombre indoor cultivation, simulate horseradish natural growthing condition well, growth for horseradish provides most suitable growing environment, owing to having carried out hardening process before transplanting, substantially increase the tolerance of horseradish to uncomfortable environment, effectively can improve the survival rate of horseradish; And indoor cultivation reduces the generation of horseradish damage by disease and insect, thus reduce the amount of labour of cultivation horseradish, effect reduces the production cost of horseradish;
(2) the inventive method is owing to adopting the hydroponic system of circulation, a culture fluid is changed every 15 ~ 30 days, can effectively control bacteria breed in water, avoid the phenomenon because bacteria breed causes horseradish root system to rot, owing to often changing culture fluid, water quality relative clean, meets problem high to water quality requirement in horseradish growth.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
A soilless culture method for horseradish, comprises the steps:
(1) installation of culturing device
In the culturing room of cool, damp, being 16cm by diameter is arranged on the high support of overhead 20cm with the cultivation tube side-by-side of field planting hole, then tube connector is utilized to be connected by cultivation pipe head and the tail, be provided with infusion pump in the front end of first cultivation pipe, finally with tube connector, cultivation pipe, liquid storage tank, infusion pump be connected to form the hydroponic system that a culture fluid can circulate; Distance between two adjacent field planting holes is 30cm; In culturing room, provided with fan carrys out the gas permeability in auxiliary control room;
(2) horseradish plantlet is cultivated
A
1select the horseradish kind needing to carry out cultivating, seed is kept 18 DEG C, humidity is 90%, and short its germinates;
A
2when wasabi seed germination length is 1cm, cut stem apex, length is 0.3cm;
A
3by steps A
2after the bud cleaning put in order, with gauze parcel, utilize running water continual rinsing 2 hours, then soak 30min, finally with aseptic this suck dry moisture of moisture absorption paper substrate with the carbendazim solution of 1% concentration;
A
4will through steps A
3obtained horseradish bud stem apex utilizes alcohol-pickled 28 seconds of 70% volumetric concentration, then utilizes 0.2%HgCl
2carry out surface sterilization, finally use sterile water wash 5 times;
A
5medium is utilized to cultivate into aseptic horseradish plantlet the stem apex after sterilizing;
A
6the shoot apical meristem of educating through being commissioned to train at the beginning of sterilizing detoxification is transferred in the 1/2MS medium not adding hormone and grows;
Wherein, be added with the root-growing agent that concentration is 0.3mg/L in 1/2MS medium, root-growing agent is IBA;
(3) hardening
Utilize above-mentioned steps A
6medium continue, the amount of medium was removed the half to only having initial medium amount every 2 days by period, cultivated 1 day, was then filling it up with medium, so circulating, and cultivated and completed hardening after 20 days;
(4) transplant
First will in culture apparatus, utilize infusion pump to pump into horseradish special culture solution, allow its culture fluid be full of cultivation pipe; Take out the horseradish seedling after hardening, utilize running water to wash away the medium in horseradish shoot root portion, the root then utilizing sponge to wrap horseradish seedling is put into culture hole and is cultivated, and the transfusion speed simultaneously controlling infusion pump avoids culture fluid flow velocity to rush askew by horseradish seedling soon;
(5) manage
After horseradish seedling survives, a horseradish special culture solution is changed every 15 days, then joint carries out the process of turk platform during the blossom season, during turk platform, base portion stays 3cm to protect stem, control to cultivate indoor temperature 18 DEG C, humidity 60%, the intensity of illumination aqueous solution that to be 2000Lux horseradish special culture solution be containing horseradish special fertilizer, the part by weight of special fertilizer and water is 1:50.
Embodiment 2
A soilless culture method for horseradish, comprises the steps:
(1) installation of culturing device
In the culturing room of cool, damp, being 18cm by diameter is arranged on the high support of overhead 25cm with the cultivation tube side-by-side of field planting hole, then tube connector is utilized to be connected by cultivation pipe head and the tail, be provided with infusion pump in the front end of first cultivation pipe, finally with tube connector, cultivation pipe, liquid storage tank, infusion pump be connected to form the hydroponic system that a culture fluid can circulate; Distance between two adjacent field planting holes is 35cm; In culturing room, provided with fan carrys out the gas permeability in auxiliary control room;
(2) horseradish plantlet is cultivated
A
1select the horseradish kind needing to carry out cultivating, seed is kept 20 DEG C, humidity is 90%, and short its germinates;
A
2when wasabi seed germination length is 1.5cm, cut stem apex, length is 0.4cm;
A
3by steps A
2after the bud cleaning put in order, with gauze parcel, utilize running water continual rinsing 2 hours, then soak 30min, finally with aseptic this suck dry moisture of moisture absorption paper substrate with the carbendazim solution of 1% concentration;
A
4will through steps A
3obtained horseradish bud stem apex utilizes alcohol-pickled 31 seconds of 70% volumetric concentration, then utilizes 0.2%HgCl
2carry out surface sterilization, finally use sterile water wash 5 times;
A
5medium is utilized to cultivate into aseptic horseradish plantlet the stem apex after sterilizing;
A
6the shoot apical meristem of educating through being commissioned to train at the beginning of sterilizing detoxification is transferred in the 1/2MS medium not adding hormone and grows;
Wherein, be added with the root-growing agent that concentration is 0.4mg/L in 1/2MS medium, root-growing agent is each half of IBA and IAA;
(3) hardening
Utilize above-mentioned steps A
6medium continue, the amount of medium was removed the half to only having initial medium amount every 3 days by period, cultivated 2 days, was then filling it up with medium, so circulating, and cultivated and completed hardening after 24 days;
(4) transplant
First will in culture apparatus, utilize infusion pump to pump into horseradish special culture solution, allow its culture fluid be full of cultivation pipe; Take out the horseradish seedling after hardening, utilize running water to wash away the medium in horseradish shoot root portion, the root then utilizing sponge to wrap horseradish seedling is put into culture hole and is cultivated, and the transfusion speed simultaneously controlling infusion pump avoids culture fluid flow velocity to rush askew by horseradish seedling soon;
(5) manage
After horseradish seedling survives, changed a horseradish special culture solution every 24 days, then joint carries out the process of turk platform during the blossom season, and during turk platform, base portion stays 4cm to protect stem, and control cultivation indoor temperature 20 DEG C, humidity 70%, intensity of illumination are 2200Lux.
Horseradish special culture solution is the aqueous solution containing horseradish special fertilizer, and the part by weight of special fertilizer and water is 1.5:50.
Embodiment 3
A soilless culture method for horseradish, comprises the steps:
(1) installation of culturing device
In the culturing room of cool, damp, being 20cm by diameter is arranged on the high support of overhead 30cm with the cultivation tube side-by-side of field planting hole, then tube connector is utilized to be connected by cultivation pipe head and the tail, be provided with infusion pump in the front end of first cultivation pipe, finally with tube connector, cultivation pipe, liquid storage tank, infusion pump be connected to form the hydroponic system that a culture fluid can circulate; Distance between two adjacent field planting holes is 40cm; In culturing room, provided with fan carrys out the gas permeability in auxiliary control room;
(2) horseradish plantlet is cultivated
A
1select the horseradish kind needing to carry out cultivating, seed is kept 22 DEG C, humidity is 90%, and short its germinates;
A
2when wasabi seed germination length is 2cm, cut stem apex, length is 0.4cm;
A
3by steps A
2after the bud cleaning put in order, with gauze parcel, utilize running water continual rinsing 2 hours, then soak 30min, finally with aseptic this suck dry moisture of moisture absorption paper substrate with the carbendazim solution of 1% concentration;
A
4will through steps A
3obtained horseradish bud stem apex utilizes alcohol-pickled 32 seconds of 70% volumetric concentration, then utilizes 0.2%HgCl
2carry out surface sterilization, finally use sterile water wash 5 times;
A
5medium is utilized to cultivate into aseptic horseradish plantlet the stem apex after sterilizing;
A
6the shoot apical meristem of educating through being commissioned to train at the beginning of sterilizing detoxification is transferred in the 1/2MS medium not adding hormone and grows;
Wherein, be added with the root-growing agent that concentration is 0.4mg/L in 1/2MS medium, root-growing agent is IAA;
(3) hardening
Utilize above-mentioned steps A
6medium continue, the amount of medium was removed the half to only having initial medium amount every 3 days by period, cultivated 2 days, was then filling it up with medium, so circulating, and cultivated and completed hardening after 27 days;
(4) transplant
First will in culture apparatus, utilize infusion pump to pump into horseradish special culture solution, allow its culture fluid be full of cultivation pipe; Take out the horseradish seedling after hardening, utilize running water to wash away the medium in horseradish shoot root portion, the root then utilizing sponge to wrap horseradish seedling is put into culture hole and is cultivated, and the transfusion speed simultaneously controlling infusion pump avoids culture fluid flow velocity to rush askew by horseradish seedling soon;
(5) manage
After horseradish seedling survives, changed a horseradish special culture solution every 30 days, then joint carries out the process of turk platform during the blossom season, and during turk platform, base portion stays 5cm to protect stem, and control cultivation indoor temperature 23 DEG C, humidity 80%, intensity of illumination are 2400Lux; Horseradish special culture solution is the aqueous solution containing horseradish special fertilizer, and the part by weight of special fertilizer and water is 2:50.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. a soilless culture method for horseradish, is characterized in that, comprises the steps:
(1) installation of culturing device
In the culturing room of cool, damp, being 16 ~ 20cm by diameter is arranged on the high support of overhead 20 ~ 30cm with the cultivation tube side-by-side of field planting hole, then tube connector is utilized to be connected by cultivation pipe head and the tail, be provided with infusion pump in the front end of first cultivation pipe, finally with tube connector, cultivation pipe, liquid storage tank, infusion pump be connected to form the hydroponic system that a culture fluid can circulate;
(2) horseradish plantlet is cultivated
A
1select the horseradish kind needing to carry out cultivating, seed is kept 18 ~ 22 DEG C, and humidity is 90%, and short its germinates;
A
2when wasabi seed germination length is 1 ~ 2cm, cut stem apex, length is 0.3 ~ 0.4cm;
A
3by steps A
2after the bud cleaning put in order, with gauze parcel, utilize running water continual rinsing 2 hours, then soak 30min, finally with aseptic this suck dry moisture of moisture absorption paper substrate with the carbendazim solution of 1% concentration;
A
4will through steps A
3obtained horseradish bud stem apex utilizes alcohol-pickled 28 ~ 32 seconds of 70% volumetric concentration, then utilizes 0.2%HgCl
2carry out surface sterilization, finally use sterile water wash 5 times;
A
5medium is utilized to cultivate into aseptic horseradish plantlet the stem apex after sterilizing;
A
6the shoot apical meristem of educating through being commissioned to train at the beginning of sterilizing detoxification is transferred in the 1/2MS medium not adding hormone and grows;
(3) hardening
Utilize above-mentioned steps A
6medium continue, the amount of medium was removed the half to only having initial medium amount every 2 ~ 3 days by period, cultivated 1 ~ 2 day, was then filling it up with medium, so circulating, and cultivated and completed hardening after 20 ~ 27 days;
(4) transplant
First will in culture apparatus, utilize infusion pump to pump into horseradish special culture solution, allow its culture fluid be full of cultivation pipe; Take out the horseradish seedling after hardening, utilize running water to wash away the medium in horseradish shoot root portion, the root then utilizing sponge to wrap horseradish seedling is put into culture hole and is cultivated, and the transfusion speed simultaneously controlling infusion pump avoids culture fluid flow velocity to rush askew by horseradish seedling soon;
(5) manage
After horseradish seedling survives, changed a horseradish special culture solution every 15 ~ 30 days, then joint carries out the process of turk platform, during turk platform during the blossom season, base portion stays 3 ~ 5cm to protect stem, and control cultivation indoor temperature 18 ~ 23 DEG C, humidity 60 ~ 80%, intensity of illumination are 2000 ~ 2400Lux.
2. the soilless culture method of a kind of horseradish according to claim 1, it is characterized in that, in step (1), the distance between two adjacent described field planting holes is 30 ~ 40cm.
3. the soilless culture method of a kind of horseradish according to claim 1, is characterized in that, steps A
6in, be added with the root-growing agent that concentration is 0.3 ~ 0.5mg/L in described 1/2MS medium, root-growing agent is one in IBA, NAA, IAA or its mixture.
4. the soilless culture method of a kind of horseradish according to claim 1, it is characterized in that, in step (5), described horseradish special culture solution is the aqueous solution containing horseradish special fertilizer, and the part by weight of special fertilizer and water is 1 ~ 2:50.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410263567.8A CN105309287A (en) | 2014-06-14 | 2014-06-14 | Soilless culture method for horseradish |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410263567.8A CN105309287A (en) | 2014-06-14 | 2014-06-14 | Soilless culture method for horseradish |
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| CN105309287A true CN105309287A (en) | 2016-02-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111466285A (en) * | 2020-04-20 | 2020-07-31 | 成都大学 | Method for planting horseradish plants in water area environment |
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|---|---|---|---|---|
| JPS6485016A (en) * | 1987-09-24 | 1989-03-30 | New Bio Giken Kk | Aquiculture of japanese horseradish |
| JP2008011789A (en) * | 2006-07-06 | 2008-01-24 | Daitsu:Kk | Cultivation method and device for japanese horseradish |
| JP2008118922A (en) * | 2006-11-13 | 2008-05-29 | Daitsu:Kk | Method for culturing wasabia japonica and culture apparatus |
| JP4314316B1 (en) * | 2008-12-26 | 2009-08-12 | 日本グリーンファーム株式会社 | Plant cultivation equipment for home use |
| CN102668992A (en) * | 2012-06-28 | 2012-09-19 | 井冈山大学 | Method for rooting of horseradish outside tissue culture seedling bottle |
| CN103125386A (en) * | 2013-02-04 | 2013-06-05 | 四川蓝公府农业科技有限公司 | Industrial horseradish planting method |
| JP2013158284A (en) * | 2012-02-03 | 2013-08-19 | Shinshu Univ | Germination method of wasabi seed |
| CN203424100U (en) * | 2013-08-27 | 2014-02-12 | 张志远 | Pipeline type hydroponic flower system |
-
2014
- 2014-06-14 CN CN201410263567.8A patent/CN105309287A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6485016A (en) * | 1987-09-24 | 1989-03-30 | New Bio Giken Kk | Aquiculture of japanese horseradish |
| JP2008011789A (en) * | 2006-07-06 | 2008-01-24 | Daitsu:Kk | Cultivation method and device for japanese horseradish |
| JP2008118922A (en) * | 2006-11-13 | 2008-05-29 | Daitsu:Kk | Method for culturing wasabia japonica and culture apparatus |
| JP4314316B1 (en) * | 2008-12-26 | 2009-08-12 | 日本グリーンファーム株式会社 | Plant cultivation equipment for home use |
| JP2013158284A (en) * | 2012-02-03 | 2013-08-19 | Shinshu Univ | Germination method of wasabi seed |
| CN102668992A (en) * | 2012-06-28 | 2012-09-19 | 井冈山大学 | Method for rooting of horseradish outside tissue culture seedling bottle |
| CN103125386A (en) * | 2013-02-04 | 2013-06-05 | 四川蓝公府农业科技有限公司 | Industrial horseradish planting method |
| CN203424100U (en) * | 2013-08-27 | 2014-02-12 | 张志远 | Pipeline type hydroponic flower system |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111466285A (en) * | 2020-04-20 | 2020-07-31 | 成都大学 | Method for planting horseradish plants in water area environment |
| CN111466285B (en) * | 2020-04-20 | 2022-01-11 | 成都大学 | Method for planting horseradish plants in water area environment |
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Application publication date: 20160210 |