CN105296140A - Method based on Maillard reaction for preparing strong aromatic flaxseed oil - Google Patents
Method based on Maillard reaction for preparing strong aromatic flaxseed oil Download PDFInfo
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Abstract
The invention discloses a method based on a Maillard reaction for preparing strong aromatic flaxseed oil. The method is characterized by comprising the following steps: pretreatment of raw materials, enzymolysis, centrifugation, Maillard reaction, and blending. An enzymatic hydrolysis solution of flaxseed meal is obtained through enzymolysis and centrifugation; the enzymatic hydrolysis solution of the flaxseed meal together with glucose and fructose is subjected to the Maillard reaction in a glycerin system to obtain a Maillard reaction product; and the Maillard reaction product is mixed with flaxseed oil according to a ratio to obtain Maillard reaction flaxseed oil. Compared with the prior art, the method has characteristics of simple process, convenience, good color and luster of a finished product, and strong aroma.
Description
Technical field
The present invention relates to field of food, relate generally to a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction.
Background technology
Edible oil is people's daily life requisite, and along with the raising of people's living standard, people require also more and more higher to edible oil quality.General edible oil quality is made up of multiple factors, and as local flavor, nutrition, color and luster and functionally active etc., wherein, fragrance is the sensory attribute of edible vegetable oil most feature, its difference because of oil variety, the growth condition such as weather and processing conditions and difference to some extent.As everyone knows, the good and bad odour characteristics also had with it of most edible oil quality is closely bound up.At present, people, on the basis of resolving adequate food and clothing, start fragrance and the nutrition of paying close attention to edible oil, and edible oil kind is also progressively developed by the general layout of single oil product to diversification, nutrient laden, functionalization.Therefore, green, healthy edible oil industry plays very important effect as a Chaoyang industry in people's daily life.Siritch is because having strong, unique fragrance and abundant nutritive value effect and being familiar with by increasing people, and its fragrance and local flavor meet the food habits of majority, especially in Xinjiang, Qinghai, Gansu, the provinces and regions, northwest such as Inner Mongol and Ningxia are rather well received.Along with progress and the development of food scientific technology, the research such as the Changing Pattern in the analysis and identification of siritch characteristic aroma substance, the course of processing and Forming Mechanism thereof causes the concern of many investigators.
At present, Flax Output Index occupies the 7th in the large oil crops rank in the world ten, and the country of plant development flax mainly contains China, Canada, Argentina and Russian etc.China's flax plantation is mainly distributed in each province, the Northwest, it is local main plant oil source, annual cultivated area about 1,000,000 hectares, with Xinjiang, the Inner Mongol, Gansu, provinces and regions, Shanxi four output maximum (about 420,000 tons/year), accounts for 75% of national ultimate production.
Flax fruit is capsule, spherical in shape, splits when top is ripe, and each fruit is containing about 10, seed.Flaxseed is primarily of seed coat, endosperm and cotyledon composition, and color is divided into brown and yellow, full seed Average oil concentration 30% ~ 40%.Because alpha-linolenic acid in siritch accounts for about 50% of total fatty acid content, therefore, flaxseed is the oil crops that alpha-linolenic acid content is the highest.
Xinjiang is one of six large flax producing regions, the whole nation, is also one of provinces and regions that national flax per unit area yield is higher, annual plantation flax 25 ~ 350,000 mu.2005 ~ 2007 annual per mu yields 86.9 kilograms, higher 17.4 kilograms than the whole nation average yield per mu 69.5 kilograms same period, exceed 25%.Xinjiang Flax is produced more than 80% area and is concentrated on Kazak Autonomous Prefecture of Ili, meadow, Yi Li state, forest coverage 67.7%, annual surface water yielding flow 164 billion cubic meter, due to the difference of sea level elevation between territory, county, eight Xian Er city year sunshine times are between 2600 ~ 3000 hours, more than 10 DEG C annual accumution reach 1600 ~ 3500 DEG C, about 100 days ~ 170 days frostless season, quantity of precipitation 200 ~ 500 millimeters.Yi Li state is main Foodgrain and oilseed production base, Xinjiang, flax essential species is implanted in the dry land in piedmont hill area, the Mountain Ranges of Tian Shan Mountains, cultivated area reaches 500,000 mu, varieties of plant mainly contains " No. 1, Yi Ya ", " No. 3, Yi Ya ", " No. 4, Yi Ya ", " Gansu Province sub-No. 7 ", " sub-No. 8 of Gansu Province ", the kind such as " fixed sub-No. 17 " and " No. 6, Asia, sky ", wherein, only just about 20,000 hm2 are reached in Ili Prefecture periphery seven county's flax cultivated areas.In recent years, the domestic development research to flaxseed also obtains certain progress, as the flax edible health-care oil, alpha-linolenic acid Capsules of health productions, lignan etc. of domestic-developed, but its goods market need further develop and spread, therefore flax deep processing and comprehensive utilization market potential huge.
Linseed oil is extracted from flaxseed by different process method to obtain, and its main technique evaluation index is the extraction yield of oil.At present, linseed oil main preparation methods has mechanical milling process, solvent extraction method, biological enzyme and supercritical extraction etc., and applying more in actual production is mechanical expression method and solvent extraction method.
Milling process refers to that grease is directly extruded the method separated by the simple physical pressure that adopts from oil plant, is mainly divided into hot moulding and cold press two kinds of methods.Before hot moulding liquefaction, need raw material to stir-fry to there being strong fragrance to produce and squeeze, the method oil yield is relatively high and give off a strong fragrance, but liquefaction color and luster is darker; Cold press then directly extrudes liquefaction under field conditions (factors), the method can at utmost ensure seed oil natural nutrient component be not damaged, but slightly light relative to its fragrance of hot moulding method, oil yield is lower.Sun Dongwei etc. first by the flax raw material that screened through strip plate, then steam at 40 ~ 60 DEG C, fry base, then normal temperature squeezes to obtain low temperature crude oil and grouts, then to carry out after multiple squeezing, anhydrous dephosphorization obtaining low-temperature cold pressing siritch.Liu Hongju etc. also report the technological method of the highest a kind of oil outlet temperature lower than the low-temperature cold pressing siritch of 80 DEG C, and all refining process adopts dry method or physisorphtion to carry out at lower than the temperature of 40 DEG C.
Solvent extraction method refers to and adopts suitable organic solvent by spraying oil plant and soaking, and makes grease in oil plant by solvent extraction out, then through precipitation, come unstuck, dewater, decolour, deodorization, the step such as depickling obtain lixiviate oil.Solvent extraction method is relative to the above two, and oil yield significantly improves, but grease fragrance is relatively thin, also may there is dissolvent residual.Linseed oil extracts the mode adopting milling process and extraction to combine more to carry out, to extract the grease in flaxseed to greatest extent.
Therefore, it is simple that one has technique, and method is convenient, and finished product color and luster is good, and oil yield is high, and the siritch preparation method that fragrance is dense is suggested.
Summary of the invention
The object of this invention is to provide a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction.
The invention discloses a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps:
A, raw materials pretreatment: get the 100 weight part flaxseed dregs of rice and mix with 300 ~ 500 weight parts waters, process 10 ~ 30min, be then cooled to 45-50 DEG C in 60 ~ 100 DEG C of water-baths, regulates pH to 7.3 ~ 9, obtain the pre-treatment material of concentration of substrate 25% ~ 35%;
B, enzymolysis: the Sumizyme MP adding 30000 ~ 40000U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4 ~ 5h, high temperature once goes out enzyme be cooled to 40 ~ 60 DEG C afterwards, regulate pH to 6 ~ 6.8, the flavor protease adding 2500 ~ 3000U again carries out secondary enzymolysis, the secondary enzymolysis time is 3 ~ 4h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, gets supernatant liquid, namely obtains flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1 ~ 2 weight part and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 40 ~ 50 weight part step c and 80 ~ 120 weight parts in reaction vessel, under 15 ~ 25KHz condition, supersound process 8 ~ 12min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.5 ~ 3h, still internal pressure 18 ~ 23Mpa, pH is 7.0-7.5, temperature 120 ~ 130 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:80 ~ 150 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch.
The flaxseed dregs of rice of above-mentioned a step are pulverized, grinding particle size≤30 order.
Above-mentioned step c, centrifugal under 3000 ~ 5000r/min condition centrifugal 10 ~ 20min.
Above-mentioned step e, siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Compared with prior art, it is simple that the present invention has technique, and method is convenient, the features such as finished product color and luster is good, and oil yield is high, and fragrance is dense.
Embodiment
Embodiment 1: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 2 object flaxseed dregs of rice mix with 300 weight parts waters, 10min is processed in 60 DEG C of water-baths, then 45 DEG C are cooled to, regulate pH to 7.3, obtain the pre-treatment material of concentration of substrate 25%;
B, enzymolysis: the Sumizyme MP adding 30000U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4h, high temperature once goes out enzyme be cooled to 40 DEG C afterwards, regulate pH to 6, the flavor protease adding 2500U again carries out secondary enzymolysis, the secondary enzymolysis time is 3h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3080r/min condition centrifugal 10min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1 weight part and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 40 weight part step c and 80 weight parts in reaction vessel, under 15KHz condition, supersound process 8min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.5h, still internal pressure 18Mpa, pH is 7, temperature 120 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:80 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 2: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 4 object flaxseed dregs of rice mix with 500 weight parts waters, 30min is processed in 100 DEG C of water-baths, then 50 DEG C are cooled to, regulate pH to 9, obtain the pre-treatment material of concentration of substrate 35%;
B, enzymolysis: the Sumizyme MP adding 30040U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 5h, high temperature once goes out enzyme be cooled to 60 DEG C afterwards, regulate pH to 6.8, the flavor protease adding 2500U again carries out secondary enzymolysis, the secondary enzymolysis time is 4h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3160r/min condition centrifugal 20min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 2 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 50 weight part step c and 82 weight parts in reaction vessel, under 25KHz condition, supersound process 8.2min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.55h, still internal pressure 18.2Mpa, pH is 7.2, temperature 124 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:82 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 3: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 6 object flaxseed dregs of rice mix with 308 weight parts waters, 12min is processed in 62 DEG C of water-baths, then 45.3 DEG C are cooled to, regulate pH to 7.5, obtain the pre-treatment material of concentration of substrate 25.2%;
B, enzymolysis: the Sumizyme MP adding 30080U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.1h, high temperature once goes out enzyme be cooled to 42 DEG C afterwards, regulate pH to 6.1, the flavor protease adding 2520U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.1h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3240r/min condition centrifugal 11min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.1 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 41 weight part step c and 84 weight parts in reaction vessel, under 15.2KHz condition, supersound process 8.4min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.51h, still internal pressure 18.4Mpa, pH is 7.4, temperature 126 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:84 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 4: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 8 object flaxseed dregs of rice mix with 316 weight parts waters, 29min is processed in 98 DEG C of water-baths, then 1.3 DEG C are cooled to, regulate pH to 8.8, obtain the pre-treatment material of concentration of substrate 34.9%;
B, enzymolysis: the Sumizyme MP adding 30120U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.3h, high temperature once goes out enzyme be cooled to 59 DEG C afterwards, regulate pH to 6.6, the flavor protease adding 2540U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.3h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3320r/min condition centrifugal 18min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.5 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 43 weight part step c and 86 weight parts in reaction vessel, under 15.4KHz condition, supersound process 8.05min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.52h, still internal pressure 18.6Mpa, pH is 7.02, temperature 128 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:86 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 5: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 10 object flaxseed dregs of rice mix with 324 weight parts waters, 14min is processed in 64 DEG C of water-baths, then 45.5 DEG C are cooled to, regulate pH to 7.5, obtain the pre-treatment material of concentration of substrate 25.4%;
B, enzymolysis: the Sumizyme MP adding 30160U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.5h, high temperature once goes out enzyme be cooled to 44 DEG C afterwards, regulate pH to 6.3, the flavor protease adding 2560U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.5h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3400r/min condition centrifugal 13min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.7 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 45 weight part step c and 88 weight parts in reaction vessel, under 15.6KHz condition, supersound process 8.05min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.53h, still internal pressure 18.8Mpa, pH is 7.04, temperature 130 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:88 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 6: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 12 object flaxseed dregs of rice mix with 332 weight parts waters, 27min is processed in 96 DEG C of water-baths, then 48.6 DEG C are cooled to, regulate pH to 8.6, obtain the pre-treatment material of concentration of substrate 34.7%;
B, enzymolysis: the Sumizyme MP adding 30200U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.3h, high temperature once goes out enzyme be cooled to 57 DEG C afterwards, regulate pH to 6.4, the flavor protease adding 2580U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.05h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3480r/min condition centrifugal 16min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.9 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 47 weight part step c and 90 weight parts in reaction vessel, under 15.8KHz condition, supersound process 9min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 3h, still internal pressure 19Mpa, pH is 7.06, temperature 120.2 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:90 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 7: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 14 object flaxseed dregs of rice mix with 340 weight parts waters, 16min is processed in 66 DEG C of water-baths, then 45.7 DEG C are cooled to, regulate pH to 7.7, obtain the pre-treatment material of concentration of substrate 25.6%;
B, enzymolysis: the Sumizyme MP adding 30240U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.4h, high temperature once goes out enzyme be cooled to 46 DEG C afterwards, regulate pH to 6.5, the flavor protease adding 2600U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.15h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3560r/min condition centrifugal 15min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.2 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 49 weight part step c and 92 weight parts in reaction vessel, under 16KHz condition, supersound process 9.5min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.55h, still internal pressure 19.2Mpa, pH is 7.08, temperature 120.4 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:92 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 8: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 16 object flaxseed dregs of rice mix with 348 weight parts waters, 25min is processed in 94 DEG C of water-baths, then 48.4 DEG C are cooled to, regulate pH to 8.4, obtain the pre-treatment material of concentration of substrate 34.5%;
B, enzymolysis: the Sumizyme MP adding 30280U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.2h, high temperature once goes out enzyme be cooled to 55 DEG C afterwards, regulate pH to 6.2, the flavor protease adding 2620U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.25h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3640r/min condition centrifugal 14min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.4 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 42 weight part step c and 94 weight parts in reaction vessel, under 16.2KHz condition, supersound process 10min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.6h, still internal pressure 19.4Mpa, pH is 7.1, temperature 120.6 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:94 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 9: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 18 object flaxseed dregs of rice mix with 356 weight parts waters, 18min is processed in 68 DEG C of water-baths, then 45.9 DEG C are cooled to, regulate pH to 7.9, obtain the pre-treatment material of concentration of substrate 25.8%;
B, enzymolysis: the Sumizyme MP adding 30320U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.12h, high temperature once goes out enzyme be cooled to 48 DEG C afterwards, regulate pH to 6.7, the flavor protease adding 2640U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.35h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3720r/min condition centrifugal 17min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.6 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 44 weight part step c and 96 weight parts in reaction vessel, under 16.4KHz condition, supersound process 11min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.52h, still internal pressure 19.6Mpa, pH is 7.12, temperature 120.8 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:96 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 10: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 20 object flaxseed dregs of rice mix with 364 weight parts waters, 23min is processed in 90 DEG C of water-baths, then 48 DEG C are cooled to, regulate pH to 8.2, obtain the pre-treatment material of concentration of substrate 34.3%;
B, enzymolysis: the Sumizyme MP adding 30360U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.4h, high temperature once goes out enzyme be cooled to 53 DEG C afterwards, regulate pH to 6.15, the flavor protease adding 2660U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.04h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3800r/min condition centrifugal 12min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.8 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 46 weight part step c and 98 weight parts in reaction vessel, under 16.6KHz condition, supersound process 10.5min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.53h, still internal pressure 19.8Mpa, pH is 7.14, temperature 120.1 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:98 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 11: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 22 object flaxseed dregs of rice mix with 372 weight parts waters, 20min is processed in 70 DEG C of water-baths, then 46.1 DEG C are cooled to, regulate pH to 8, obtain the pre-treatment material of concentration of substrate 26;
B, enzymolysis: the Sumizyme MP adding 30400U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.3h, high temperature once goes out enzyme be cooled to 50 DEG C afterwards, regulate pH to 6.25, the flavor protease adding 2680U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.14h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3880r/min condition centrifugal 19min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 2 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 48 weight part step c and 100 weight parts in reaction vessel, under 16.8KHz condition, supersound process 11.5min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.5h, still internal pressure 20Mpa, pH is 7.16, temperature 120.3 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:100 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 12: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 24 object flaxseed dregs of rice mix with 380 weight parts waters, 21min is processed in 88 DEG C of water-baths, then 47.8 DEG C are cooled to, regulate pH to 7.2, obtain the pre-treatment material of concentration of substrate 34.1%;
B, enzymolysis: the Sumizyme MP adding 30440U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.14h, high temperature once goes out enzyme be cooled to 51 DEG C afterwards, regulate pH to 6.35, the flavor protease adding 2700U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.24h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4040r/min condition centrifugal 10.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.02 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 41.5 weight part step c and 102 weight parts in reaction vessel, under 17KHz condition, supersound process 11.2min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 3h, still internal pressure 20.2Mpa, pH is 7.18, temperature 120.5 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:102 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 13: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 26 object flaxseed dregs of rice mix with 388 weight parts waters, 22min is processed in 72 DEG C of water-baths, then 46.3 DEG C are cooled to, regulate pH to 7.4, obtain the pre-treatment material of concentration of substrate 26.2%;
B, enzymolysis: the Sumizyme MP adding 30480U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.16h, high temperature once goes out enzyme be cooled to 52 DEG C afterwards, regulate pH to 6.45, the flavor protease adding 2720U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.03h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4120r/min condition centrifugal 19.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.04 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 42.5 weight part step c and 104 weight parts in reaction vessel, under 17.2KHz condition, supersound process 11.4min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.52h, still internal pressure 20.4Mpa, pH is 7.20, temperature 120.7 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:105 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 14: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 28 object flaxseed dregs of rice mix with 396 weight parts waters, 19min is processed in 86 DEG C of water-baths, then 47.6 DEG C are cooled to, regulate pH to 7.6, obtain the pre-treatment material of concentration of substrate 33.9%;
B, enzymolysis: the Sumizyme MP adding 30520U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.18h, high temperature once goes out enzyme be cooled to 49 DEG C afterwards, regulate pH to 6.55, the flavor protease adding 2740U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.13h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4200r/min condition centrifugal 11.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.06 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 43.5 weight part step c and 106 weight parts in reaction vessel, under 17.4KHz condition, supersound process 10.5min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 3h, still internal pressure 20.6Mpa, pH is 7.21, temperature 120.9 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:108 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 15: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 30 object flaxseed dregs of rice mix with 404 weight parts waters, 24min is processed in 74 DEG C of water-baths, then 46.5 DEG C are cooled to, regulate pH to 7.8, obtain the pre-treatment material of concentration of substrate 26.4%;
B, enzymolysis: the Sumizyme MP adding 30560U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.22h, high temperature once goes out enzyme be cooled to 54 DEG C afterwards, regulate pH to 6.65, the flavor protease adding 2760U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.12h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4280r/min condition centrifugal 18.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.08 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 44.5 weight part step c and 108 weight parts in reaction vessel, under 17.6KHz condition, supersound process 10.3min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.8h, still internal pressure 20.8Mpa, pH is 7.22, temperature 121.2 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:115 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 16: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 5 object flaxseed dregs of rice mix with 412 weight parts waters, 17min is processed in 84 DEG C of water-baths, then 47.4 DEG C are cooled to, regulate pH to 7.35, obtain the pre-treatment material of concentration of substrate 33.7%;
B, enzymolysis: the Sumizyme MP adding 30600U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.24h, high temperature once goes out enzyme be cooled to 47 DEG C afterwards, regulate pH to 6.75, the flavor protease adding 2780U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.32h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4360r/min condition centrifugal 12.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.12 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 45.5 weight part step c and 110 weight parts in reaction vessel, under 17.8KHz condition, supersound process 10.1min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.7h, still internal pressure 21Mpa, pH is 7.24, temperature 121.3 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:118 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 17: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 15 object flaxseed dregs of rice mix with 420 weight parts waters, 26min is processed in 76 DEG C of water-baths, then 46.7 DEG C are cooled to, regulate pH to 7.45, obtain the pre-treatment material of concentration of substrate 26.2%;
B, enzymolysis: the Sumizyme MP adding 30640U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.26h, high temperature once goes out enzyme be cooled to 56 DEG C afterwards, regulate pH to 6.24, the flavor protease adding 2800U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.24h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4440r/min condition centrifugal 17.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.14 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 46.5 weight part step c and 112 weight parts in reaction vessel, under 18KHz condition, supersound process 9.1min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.56h, still internal pressure 21.2Mpa, pH is 7.26, temperature 121.5 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:120 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 18: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 25 object flaxseed dregs of rice mix with 428 weight parts waters, 15min is processed in 82 DEG C of water-baths, then 47.2 DEG C are cooled to, regulate pH to 7.65, obtain the pre-treatment material of concentration of substrate 26.4%;
B, enzymolysis: the Sumizyme MP adding 30680U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.28h, high temperature once goes out enzyme be cooled to 45 DEG C afterwards, regulate pH to 6.26, the flavor protease adding 2820U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.22h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4520r/min condition centrifugal 13.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.16 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 47.5 weight part step c and 114 weight parts in reaction vessel, under 18.2KHz condition, supersound process 9.3min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.54h, still internal pressure 21.4Mpa, pH is 7.28, temperature 121.7 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:122 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 19: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 23 object flaxseed dregs of rice mix with 432 weight parts waters, 15.5min is processed in 78 DEG C of water-baths, then 46.6 DEG C are cooled to, regulate pH to 7.75, obtain the pre-treatment material of concentration of substrate 33.5%;
B, enzymolysis: the Sumizyme MP adding 30720U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.32h, high temperature once goes out enzyme be cooled to 58 DEG C afterwards, regulate pH to 6.28, the flavor protease adding 2840U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.52h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4600r/min condition centrifugal 16.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.18 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 48.5 weight part step c and 116 weight parts in reaction vessel, under 18.4KHz condition, supersound process 8.1min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.58h, still internal pressure 21.6Mpa, pH is 7.3, temperature 121.9 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:125 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 20: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 13 object flaxseed dregs of rice mix with 430 weight parts waters, 25.5min is processed in 80 DEG C of water-baths, then 47 DEG C are cooled to, regulate pH to 7.85, obtain the pre-treatment material of concentration of substrate 26.6%;
B, enzymolysis: the Sumizyme MP adding 30760U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.34h, high temperature once goes out enzyme be cooled to 43 DEG C afterwards, regulate pH to 6.32, the flavor protease adding 2860U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.55h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4680r/min condition centrifugal 14.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.21 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 49.5 weight part step c and 118 weight parts in reaction vessel, under 18.6KHz condition, supersound process 8.3min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.62h, still internal pressure 21.8Mpa, pH is 7.32, temperature 122.1 DEG C, taking out product is U.S. rad raw fragrant source product;
The ratio of 128 carries out concussion extraction aroma substance, and namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 21: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 7 object flaxseed dregs of rice mix with 438 weight parts waters, 20.2min is processed in 63 DEG C of water-baths, then 46.8 DEG C are cooled to, regulate pH to 7.95, obtain the pre-treatment material of concentration of substrate 33.3%;
B, enzymolysis: the Sumizyme MP adding 30800U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.35h, high temperature once goes out enzyme be cooled to 60 DEG C afterwards, regulate pH to 6.34, the flavor protease adding 2880U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.14h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4760r/min condition centrifugal 15.5min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.24 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 41.6 weight part step c and 120 weight parts in reaction vessel, under 18.8KHz condition, supersound process 8.5min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.63h, still internal pressure 22Mpa, pH is 7.34, temperature 122.3 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:130 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 22: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 13 object flaxseed dregs of rice mix with 446 weight parts waters, 13min is processed in 85 DEG C of water-baths, then 46.8 DEG C are cooled to, regulate pH to 8.05, obtain the pre-treatment material of concentration of substrate 26.8%;
B, enzymolysis: the Sumizyme MP adding 30840U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.45h, high temperature once goes out enzyme be cooled to 41 DEG C afterwards, regulate pH to 6.36, the flavor protease adding 2900U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.23h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4840r/min condition centrifugal 12.4min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.26 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 41.8 weight part step c and 81 weight parts in reaction vessel, under 19KHz condition, supersound process 9.5min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.65h, still internal pressure 22.2Mpa, pH is 7.36, temperature 122.5 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:80 ~ 150 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 23: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 9 object flaxseed dregs of rice mix with 454 weight parts waters, 28min is processed in 65 DEG C of water-baths, then 46.8 DEG C are cooled to, regulate pH to 8.15, obtain the pre-treatment material of concentration of substrate 33.1%;
B, enzymolysis: the Sumizyme MP adding 30880U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.42h, high temperature once goes out enzyme be cooled to 45.5 DEG C afterwards, regulate pH to 6.38, the flavor protease adding 2920U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.15h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 4920r/min condition centrifugal 15.6min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.28 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 42.4 weight part step c and 83 weight parts in reaction vessel, under 19.2KHz condition, supersound process 9.3min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.75h, still internal pressure 22.4Mpa, pH is 7.38, temperature 122.7 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:135 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 24: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 23 object flaxseed dregs of rice mix with 462 weight parts waters, 17.5min is processed in 95 DEG C of water-baths, then 45.8 DEG C are cooled to, regulate pH to 8.25, obtain the pre-treatment material of concentration of substrate 27%;
B, enzymolysis: the Sumizyme MP adding 30920U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.43h, high temperature once goes out enzyme be cooled to 55.5 DEG C afterwards, regulate pH to 6.42, the flavor protease adding 2940U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.14h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 5000r/min condition centrifugal 18.6min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.56 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 42.6 weight part step c and 85 weight parts in reaction vessel, under 19.4KHz condition, supersound process 9.2min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.85h, still internal pressure 22.6Mpa, pH is 7.4, temperature 122.9 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:140 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Embodiment 25: a kind of preparation method preparing giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps: a, raw materials pretreatment: get 100 parts by weight of crushed most the 27 object flaxseed dregs of rice mix with 470 weight parts waters, 26.5min is processed in 75 DEG C of water-baths, then 47.6 DEG C are cooled to, regulate pH to 8.35, obtain the pre-treatment material of concentration of substrate 29.5;
B, enzymolysis: the Sumizyme MP adding 30960U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4.65h, high temperature once goes out enzyme be cooled to 42.5 DEG C afterwards, regulate pH to 6.5, the flavor protease adding 2950U again carries out secondary enzymolysis, the secondary enzymolysis time is 3.5h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, centrifugal under 3020r/min condition centrifugal 13.6min, get supernatant liquid, namely obtain flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1.55 weight parts and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 42.8 weight part step c and 87 weight parts in reaction vessel, under 19.6KHz condition, supersound process 11min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.5h, still internal pressure 22.8Mpa, pH is 7.5, temperature 123.5 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:145 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch, described siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
Claims (5)
1. prepare a preparation method for giving off a strong fragrance siritch based on Maillard reaction, it is characterized in that comprising the steps:
A, raw materials pretreatment: get the 100 weight part flaxseed dregs of rice and mix with 300 ~ 500 weight parts waters, process 10 ~ 30min, be then cooled to 45-50 DEG C in 60 ~ 100 DEG C of water-baths, regulates pH to 7.3 ~ 9, obtain the pre-treatment material of concentration of substrate 25% ~ 35%;
B, enzymolysis: the Sumizyme MP adding 30000 ~ 40000U in the pre-treatment material that a step obtains carries out primary enzymolysis, enzymolysis time is 4 ~ 5h, high temperature once goes out enzyme be cooled to 40 ~ 60 DEG C afterwards, regulate pH to 6 ~ 6.8, the flavor protease adding 2500 ~ 3000U again carries out secondary enzymolysis, the secondary enzymolysis time is 3 ~ 4h, and high temperature secondary is gone out enzyme afterwards;
C, centrifugal: secondary goes out after enzyme and carries out centrifugal treating, gets supernatant liquid, namely obtains flaxseed dregs of rice enzymolysis solution;
D, Maillard reaction: the glycerine adding the glucose of 1 ~ 2 weight part and/or fructose, flaxseed dregs of rice enzymolysis solution prepared by 40 ~ 50 weight part step c and 80 ~ 120 weight parts in reaction vessel, under 15 ~ 25KHz condition, supersound process 8 ~ 12min is carried out after Homogeneous phase mixing, after process, mixture is added in reactor and react 2.5 ~ 3h, still internal pressure 18 ~ 23Mpa, pH is 7.0-7.5, temperature 120 ~ 130 DEG C, taking out product is U.S. rad raw fragrant source product;
E, mixing: the raw fragrant source product of U.S. rad Step d prepared and siritch carry out concussion according to the ratio of weight ratio 1:80 ~ 150 and extract aroma substance, namely obtain U.S. rad raw fragrant source siritch.
2. the preparation method preparing giving off a strong fragrance siritch based on Maillard reaction according to claim 1, is characterized in that: the flaxseed dregs of rice of above-mentioned a step are pulverized, grinding particle size≤30 order.
3. the preparation method preparing giving off a strong fragrance siritch based on Maillard reaction according to claim 1 and 2, is characterized in that: above-mentioned step c, centrifugal under 3000 ~ 5000r/min condition centrifugal 10 ~ 20min.
4. the preparation method preparing giving off a strong fragrance siritch based on Maillard reaction according to claim 1 and 2, it is characterized in that: above-mentioned step e, siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
5. the preparation method preparing giving off a strong fragrance siritch based on Maillard reaction according to claim 3, is characterized in that: above-mentioned step e, and siritch is the siritch that in mechanical expression, extraction, biological enzyme or supercritical extraction, wherein a kind of method is obtained.
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| CN115322827A (en) * | 2022-09-16 | 2022-11-11 | 合肥工业大学 | Preparation method of self-sourced Maillard reaction sesame oil with strong fragrance |
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| CN107212109A (en) * | 2017-05-03 | 2017-09-29 | 理星（天津）生物科技有限公司 | A kind of method that use micro passage reaction prepares giving off a strong fragrance local flavor animal oil |
| CN107969700A (en) * | 2017-11-22 | 2018-05-01 | 上海华宝孔雀香精有限公司 | A kind of giving off a strong fragrance walnut oil microcapsule and preparation method thereof |
| CN107969700B (en) * | 2017-11-22 | 2021-01-12 | 上海华宝孔雀香精有限公司 | Strong-fragrance walnut oil microcapsule and preparation method thereof |
| CN111378526A (en) * | 2018-12-28 | 2020-07-07 | 贵州中烟工业有限责任公司 | Preparation method of linseed essence |
| CN110551565A (en) * | 2019-10-09 | 2019-12-10 | 武汉轻工大学 | Processing production method of strong-fragrance pumpkin seed oil |
| CN111296816A (en) * | 2020-03-27 | 2020-06-19 | 石河子大学 | Method for preparing linseed oil fragrance-producing source product by combining oriented enzymolysis of flaxseed meal with Maillard reaction |
| CN112080340A (en) * | 2020-09-18 | 2020-12-15 | 湖南新金浩茶油股份有限公司 | Tea oil with rich flavor and preparation method thereof |
| CN112574808A (en) * | 2020-11-12 | 2021-03-30 | 山东三星玉米产业科技有限公司 | Production process for preparing sesame-flavor corn oil by enzyme method |
| CN114868808B (en) * | 2022-03-14 | 2024-04-05 | 江南大学 | Preparation method of strong-flavor beef tallow and product thereof |
| CN115322827A (en) * | 2022-09-16 | 2022-11-11 | 合肥工业大学 | Preparation method of self-sourced Maillard reaction sesame oil with strong fragrance |
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