CN105289021A - Apparatus and method for concentrating collagen solution used for articular cartilage regeneration - Google Patents
Apparatus and method for concentrating collagen solution used for articular cartilage regeneration Download PDFInfo
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- CN105289021A CN105289021A CN201510817544.1A CN201510817544A CN105289021A CN 105289021 A CN105289021 A CN 105289021A CN 201510817544 A CN201510817544 A CN 201510817544A CN 105289021 A CN105289021 A CN 105289021A
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Abstract
The present invention provides an apparatus and a method for concentrating a collagen solution, the apparatus comprises a sealed housing; at least one tray for containing a collagen solution; a motor; at least one stirrer; a vacuum pump for controlling the vacuum degree within the housing; and a temperature controller for controlling the temperature within the housing; the tray is arranged in the housing, and comprises an outer sidewall and a bottom surface; the motor is provided with a motor shaft substantially perpendicular to the tray; the stirrer is driven by the motor shaft and includes at least one blade for stirring the collagen solution, the lower edge of the blade is defined and arranged to be substantially parallel to the bottom surface of the tray to move, and the distance between the lower edge and the bottom surface of the tray is 1 to 3cm. The apparatus can be used for manufacturing of collagen in reduced cost and improved efficiency, and the produced collagen is particularly suitable for articular cartilage regeneration.
Description
Technical field
The present invention relates generally to and a kind ofly concentrates core apparatus for the collagen solution of Regeneration of Articular Cartilage and method.Specifically, the present invention relates to and use described device to concentrate the method for collagen solution under low temperature and vacuum.
Background technology
The treatment of cartilage damage and cartilage degradation is the global problem of medical circle for a long time always.Through the research of scientist, find and confirm that collagen can induce human stem cell to generate new cartilage.Although collagen has been widely used in the field such as plastic surgery, wound care, the method for its processing, extraction limit, and is not suitable for cartilage and soft tissue regeneration.
Collagen is the major structural protein existed in vertebrate.Natural collagen can obtain from various performing animal.Collagen may be dissolved in aqueous diluted acid or aqueous slkali, to form liquid phase.In solution, the concentration of collagen is higher, and the viscosity of solution is higher.Make to filter by extracting from various raw material the collagen produced, to produce medical grade material.But after filtration, the concentration of collagen is very low, such as, by weight about 0.1%.
In order to obtain the concentrated collagen solution that can be used for Regeneration of Articular Cartilage, attempting and having carried out various method.Such as, use sodium chloride from the precipitation collagen of water and collagen.The sodium chloride solution of high enrichment is added into collagen solution, to obtain 0.7M sodium chloride solution, for the object precipitated.Subsequently, the collagen of precipitation is separated by gravity.Because do not wish sodium chloride in the final collagen products for people's application, and the sodium chloride of residual high enrichment may hinder Stem cell differentiation chondroblast, so need by other removal process, the sodium chloride added in collagen manufacture process to be removed, and the quantity of the step of method can be increased like this and increase cost.Therefore, the method not using sodium chloride and concentrated collagen solution is needed.
Prior art also uses filter further from the mixture collagen of collagen and water.Use compression stress or power of extruding that mixture solution is pushed through filter surfaces, to force water to leave mixture solution, concentrated collagen solution thus.But, when the concentration of collagen is increased to 3% from 1%, or when being increased to 6% from 3%, can not work well with the compression method of filter, because mixture solution is too dense, water can not be separated from mixture solution dense like this, in this process so often do not reach the concentration required for regenerating bone or cartilage.Therefore, filter and compression stress is needed not use and the method for concentrate collagen.
Prior art heats, to be separated from collagen with the mixture of water by collagen further under being also used in high temperature (about 120 DEG C).Use heat and remove water by evaporation of water from mixture.But the shortcoming of heating is (such as, 1-2 days) consuming time, because evaporation of water carries out slowly at the end face of mixture solution.And the uneven microstructure destroying collagen of heating, is unfavorable for the growth substrate as osteocyte.Therefore, still need not use and at high temperature heat and the method for concentrate collagen.
Prior art also uses centrifugal force, to be separated by the mixture of collagen with collagen and water further.The method is based on the difference of density between water and collagen.But the density variation between water and collagen is not very large, thus use centrifugal force separate can not effectively for collagen solution, so often do not reach the concentration required for regenerating bone or cartilage.Therefore, the method for the concentrate collagen without centrifugal force is needed.
In view of above-mentioned present situation, needing to develop new does not have the method for shortcoming above-mentioned for the concentrated collagen solution that can be used for Regeneration of Articular Cartilage.
Summary of the invention
The present invention relates to the apparatus and method at low temperature and reduced under vacuum collagen solution.These apparatus and method can make the concentration of collagen solution from 0.5% being increased to 1% by weight with low cost and high efficiency, or are increased to 6% from 3%.By the collagen that such mode produces, be particularly suited for Regeneration of Articular Cartilage through verification experimental verification.
On the one hand, the invention provides the device for concentrated collagen solution, it comprises
(i) seal casinghousing;
(ii) for holding at least one pallet of collagen solution, it to be arranged in described housing and to comprise lateral wall and bottom surface;
(iii) motor, it has the motor shaft being substantially perpendicular to described pallet and arranging;
(iv) at least one agitating device, it is driven by described motor shaft and comprises at least one blade for stirring collagen solution, the lower limb of described blade is defined and the bottom surface being set to be arranged essentially parallel to described pallet is moved, and the distance between described lower limb and described tray bottom surface is 1.0 to 3.0cm;
V () is for controlling the vavuum pump of vacuum in described housing;
(vi) for controlling the temperature controller of temperature in described housing.
Preferably, the height of the lateral wall of described pallet is 2.0 to 5.0cm.
Preferably, the area of the bottom surface of described pallet is 100 to 1,000cm
2.
Preferably, the ratio of the radial dimension of the height of described lateral wall and the bottom surface of described pallet is 0.1 to 0.5.
Preferably, the shape of described pallet is square or circular; Be more preferably circular.
Preferably, described device comprises 2-20, and preferably 2-12, more preferably 2-7 pallet, the bottom surface of described pallet is parallel to each other.In addition, described device comprises multiple agitating device, wherein arranges an agitating device above each pallet.More preferably, each pallet also comprises the centre bore that madial wall and madial wall limit, and described motor shaft is arranged through the centre bore of pallet.Preferably, each agitating device comprises 1-10, is preferably 2-6, is more preferably 3 blades.
Preferably, the interval between two adjacent trays is 2 to 5cm.
Preferably, described device comprises further:
(vi) charging aperture be communicated with described pallet fluid and discharge gate, for shifting collagen solution.
Preferably, described device comprises further:
(vii) for showing the pressure gauge of pressure in described housing and the thermometer plate for showing temperature in described housing.
On the other hand, the invention provides the method using device above-mentioned to concentrate collagen solution, it comprises the steps:
A () provides initial collagen solution in pallet;
B () vacuum arranged in housing is 1,000 to 2,500Pa;
C temperature that () is arranged in housing is 7 to 21 DEG C;
D () stirs collagen solution;
E () concentrates collagen solution 8 to 16 hours at described temperature and vacuum;
F collagen products that () results are concentrated.
Preferably, the concentration of described initial collagen protein solution is by weight 0.55% to 3%.
Preferably, the speed of described stirring is 20 to 120rpm.
Preferably, the degree of depth of the initial collagen protein solution in described pallet is 3 to 8cm.
Preferably, the concentration of described concentrated collagen products is by weight 1% to 6%.
Preferably, the collagen products of results is gel film form.
Apparatus and method of the present invention can be used for concentrate collagen solution, and do not use sodium chloride, filter and compression stress, high-temperature heating or centrifugal force, and this can reduce production cost and improve the efficiency manufacturing collagen.The collagen produced is suitable for medical science, food or cosmetic formulations, is particularly useful for Regeneration of Articular Cartilage matrix used.
Accompanying drawing explanation
In order to more fully understand Characteristics and advantages of the present invention, refer now to detailed Description Of The Invention and accompanying drawing is described, and wherein:
Fig. 1 is the schematic diagram of the device of concentrated collagen solution according to an embodiment of the invention.
Detailed description of the invention
Although be discussed in detail formation and the use of the various embodiment of the present invention below, will be appreciated that the many applicable inventive concept that the invention provides and can implement in various specific environment.The detailed description of the invention discussed herein is only formed and use illustrative example of the present invention, does not limit the scope of the invention.
Fig. 1 shows according to the device 10 for concentrated collagen solution of the present invention.Device 10 for concentrated collagen solution comprise sealing housing 107, for holding 5 pallets 105 and the motor 102 of collagen solution, this motor 102 have be substantially perpendicular to pallet 105 arrange motor shaft 108.Each in housing and in them of described tray arrangement includes lateral wall 109 and bottom surface 110.The bottom surface 110 substantially parallel layout each other of pallet 105.In addition, device 10 also comprises 5 agitating devices 111 driven by motor shaft 108, wherein arranges an agitating device 111 above each pallet 105.Each agitating device 111 comprises 3 blades 104 for stirring collagen solution, described blade limits lower limb 112 and move in the bottom surface 110 being configured to be arranged essentially parallel to pallet 105, wherein, the distance between the bottom surface 110 of lower limb 112 and pallet is 3cm.The centre bore 114 that each pallet 105 also comprises madial wall 113 and limited by this madial wall 113, and motor shaft 108 is configured to the centre bore 114 through pallet 105.The height of the lateral wall 109 of pallet is 4cm.The area of the bottom surface 110 of pallet 105 is 500cm
2.The height of lateral wall 109 is 0.3 with the ratio of the radius size of tray bottom surface 110.The shape of pallet 105 is circular.Interval between two adjacent trays 105 is 3cm.
Device 10 also comprises the vavuum pump 103 for controlling vacuum in housing; For controlling the temperature controller 106 of temperature in housing; Be communicated with pallet 105 fluid, for carrying charging aperture and the discharge gate (not shown) of collagen solution; For showing the pressure gauge 101 of pressure in housing and the thermometer instrument board 101 for showing temperature in housing.
By by the embodiment only with reference to Fig. 1, the present invention is described now.In the following embodiments, pigskin is for the preparation of initial collagen protein solution.Weight measurements for measure collagen solution concentration (see, BritishPharmacopoeia, 2001).
Embodiment 1
2500g initial collagen protein solution is placed in pallet 105, each pallet 500g.In each pallet 105, the degree of depth of initial collagen protein solution is 3cm.Then vavuum pump 103 and temperature controller 106 is started until the vacuum in housing 107 reaches 1500Pa and temperature in housing 107 reaches 13 DEG C.Collagen solution is stirred 12 hours with the speed of 60rpm by agitating device 111.The concentration by weight of initial collagen protein solution is 3%.With the collagen products that the form of gel film results are concentrated.The concentration by weight of concentrated collagen products is 6%.
Embodiment 2
2500g initial collagen protein solution is placed in pallet 105, each pallet 500g.In each pallet 105, the degree of depth of initial collagen protein solution is 4cm.Then vavuum pump 103 and temperature controller 106 is started until the vacuum in housing 107 reaches 2500Pa and temperature in housing 107 reaches 21 DEG C.Collagen solution is stirred 12 hours with the speed of 60rpm by agitating device 111.The concentration by weight of initial collagen protein solution is 3%.With the collagen products that the form of gel film results are concentrated.The concentration of concentrated collagen products is (6% by weight).
Embodiment 3
5000g initial collagen protein solution is placed in pallet 105, each pallet 1000g.In each pallet 105, the degree of depth of initial collagen protein solution is 4cm.Then startup vavuum pump 103 and temperature controller 106 are until the vacuum in housing 107 reaches 1000Pa, and the temperature in housing 107 reaches 7 DEG C.Collagen solution is stirred 12 hours with the speed of 60rpm by agitating device 111.The concentration of initial collagen protein solution is by weight 3%.With the collagen products that the form of gel film results are concentrated.The concentration of concentrated collagen products is by weight 6%.
Embodiment 4
5,000g initial collagen protein solution is placed in pallet 105, each pallet 1000g.In each pallet 105, the degree of depth of initial collagen protein solution is 3cm.Then startup vavuum pump 103 and temperature controller 106 are until the vacuum in housing 107 reaches 1000Pa, and the temperature in housing 107 reaches 7 DEG C.Collagen solution is stirred 12 hours with the speed of 60rpm by agitating device 111.The concentration of initial collagen protein solution is by weight 1%.With the collagen products that the form of gel film results are concentrated.The concentration of concentrated collagen products is by weight 3%.
Can prepare and implement open and all devices of requiring and/or method and do not need excessive experiment herein according to the present invention.Although according to preferred embodiment describing apparatus and method of the present invention, but it will be apparent to those skilled in the art, modification is applicable to the step of device and/or method and method as herein described or the sequence of step, and does not deviate from concept of the present invention, spirit and scope.For those skilled in the art all these similar replacements apparent and modification be considered as limiting in such as appended claim spirit of the present invention, in scope and concept.
Claims (9)
1., for a device for concentrated collagen solution, it comprises;
(i) seal casinghousing;
(ii) for holding at least one pallet of collagen solution, it to be arranged in described housing and to comprise lateral wall and bottom surface;
(iii) motor, it has the motor shaft being substantially perpendicular to described pallet and arranging;
(iv) at least one agitating device, it is driven by described motor shaft and comprises at least one blade for stirring collagen solution, the lower limb of described blade is defined and the bottom surface being set to be arranged essentially parallel to described pallet is moved, and the distance between described lower limb and described tray bottom surface is 1.0 to 3.0cm;
V () is for controlling the vavuum pump of vacuum in described housing;
(vi) for controlling the temperature controller of temperature in described housing.
2. device according to claim 1, is characterized in that, the height of the lateral wall of described pallet is 2.0 to 5.0cm;
Preferably, the area of the bottom surface of described pallet is 100 to 1,000cm
2;
Preferably, the ratio of the radial dimension of the height of described lateral wall and the bottom surface of described pallet is 0.1 to 0.5;
Preferably, the shape of described pallet is square or circular; Be more preferably circular.
3. device according to claim 1 and 2, is characterized in that, described device comprises 2 to 20, preferably 2-12, more preferably 2-7 pallet, and the bottom surface of described pallet is substantially parallel to each other; Described device comprises multiple agitating device, wherein arranges an agitating device above each pallet;
Preferably, each pallet also comprises the centre bore that madial wall and madial wall limit, and described motor shaft is arranged through the centre bore of described pallet;
Preferably, each agitating device comprises 1-10, preferably 2-6, more preferably 3 blades;
Preferably, the interval between two adjacent trays is 2 to 5cm.
4. the device according to any one of claims 1 to 3, is characterized in that, described device comprises further:
(vii) charging aperture be communicated with described pallet fluid and discharge gate, for shifting described collagen solution;
Preferably, described device comprises further:
(viii) for showing the pressure gauge of pressure in described housing and the thermometer plate for showing temperature in described housing.
5. use the device according to any one of Claims 1-4 to concentrate the method for collagen solution, it comprises the steps:
A () provides initial collagen protein solution in described pallet;
B () vacuum arranged in described housing is 1,000 to 2,500Pa;
C temperature that () is arranged in described housing is 7 to 21 DEG C;
D () stirs described collagen solution;
E () concentrates collagen solution 8 to 16 hours at described temperature and vacuum;
F collagen products that () results are concentrated.
6. method according to claim 5, is characterized in that, the concentration of described initial collagen protein solution is by weight 0.55% to 3%.
7. the method according to claim 5 or 6, is characterized in that, the speed of described stirring is 20 to 120rpm.
8. the method according to any one of claim 5 to 7, is characterized in that, the degree of depth of the initial collagen protein solution in described pallet is 3 to 8cm.
9. the method according to any one of claim 5 to 8, is characterized in that, the concentration of described concentrated collagen products is by weight 1% to 6%;
Preferably, the collagen products of results is gel film form.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510817544.1A CN105289021B (en) | 2015-11-23 | 2015-11-23 | A kind of apparatus and method for concentrating the collagen solution for Regeneration of Articular Cartilage |
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| CN201510817544.1A CN105289021B (en) | 2015-11-23 | 2015-11-23 | A kind of apparatus and method for concentrating the collagen solution for Regeneration of Articular Cartilage |
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| CN105289021A true CN105289021A (en) | 2016-02-03 |
| CN105289021B CN105289021B (en) | 2017-08-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110063405A (en) * | 2019-05-16 | 2019-07-30 | 江苏美德实医疗科技有限公司 | A kind of device and method of industrial efficient batch continuous production collagen |
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| SU1169719A1 (en) * | 1984-02-20 | 1985-07-30 | Институт Неорганической Химии Со Ан Ссср | Phase mixing apparatus |
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| CN201094868Y (en) * | 2007-08-26 | 2008-08-06 | 冯建东 | Super short pipe-disk type vacuum rotating concentrator |
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| CN110063405A (en) * | 2019-05-16 | 2019-07-30 | 江苏美德实医疗科技有限公司 | A kind of device and method of industrial efficient batch continuous production collagen |
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| CN105289021B (en) | 2017-08-29 |
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