CN104922732B - A kind of oral biological film preparation method - Google Patents
A kind of oral biological film preparation method Download PDFInfo
- Publication number
- CN104922732B CN104922732B CN201410101451.4A CN201410101451A CN104922732B CN 104922732 B CN104922732 B CN 104922732B CN 201410101451 A CN201410101451 A CN 201410101451A CN 104922732 B CN104922732 B CN 104922732B
- Authority
- CN
- China
- Prior art keywords
- collagen
- chondroitin sulfate
- slurries
- acetic acid
- film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention relates to a kind of oral biological film preparation method, which includes being added to I-type collagen in acetic acid solution that collagen acetic acid swelling solution is made；Chondroitin sulfate is added wherein, stirring is configured to collagen-chondroitin sulfate slurries；By its vacuum freeze drying；It is pressed into collagen composite film later；Collagen-chondroitin sulfate slurries are sprayed in two layers of collagen composite film, and carry out vacuum freeze drying；High-temperature vacuum is crosslinked again later；Sterilizing obtains the compound oral biological film of collagen-chondroitin sulfate after crosslinking.It is suitable for controllable degradation absorbability, low antigenicity, good plasticity and mechanical property the invention has the benefit that there is good histocompatbility using the oral biological film of this method production.It performs the operation for the department of stomatology, three-dimensional porous structure provides tissue engineering bracket for the reparation of injury tissue, is conducive to intrusion and the differential growth of cell, can induce the reparative regeneration of defective tissue, and specific thickness can effectively prevent growing into for soft tissue.
Description
Technical field
The present invention relates to oral medical Material Field more particularly to a kind of oral biological film preparation methods.
Background technique
Collagen is a kind of biological polymer substance, English scientific name Collagen.Play the part of combination in zooblast
The role of tissue.For one of most critical raw material of biotech industry and the very huge best raw doctor of demand
Material.Its application field includes biology doctor material, cosmetics, food industry, research purposes etc..
Collagen is the structural protein of extracellular matrix, and molecule is collected as supramolecular structured in extracellular matrix
Structure.Molecular weight is 300KD.The most common structure feature of collagen is triple-helix structure.It is made of 3 a chain polypeptides, each
Collagen chain is all left hand helix configuration.3 left hand helix chain forks are mutually wound in right-handed helix structure, i.e. superhelix closes
The unique triple helices structure of collagen, keeps its molecular structure highly stable, and has low immunogenicity and good life
Object compatibility etc..Structure determines that property, property determine that purposes, the diversity and complexity of the structure of collagen determine that it is being permitted
Multi-field critical role.Collagen product has a good application prospect.
Collagen is with many merits of itself: good biocompatibility, catabolite are easily absorbed, and finally decompose ammonification
Base acid, carbon dioxide and water, become the research hotspot of used in tissue engineering biomaterial.
Chondroitin sulfate is one of extracellular matrix glycosaminoglycan, and sugar chain is by alternate glucuronic acid and N- acetyl
Galactosamine (also known as N- acetylamino galactosamine) disaccharide unit composition, is connected to core protein like sugar chain block by one
On serine residue.Chondroitin sulfate, which is present in from nematode to people, to be removed in exophytic all biologies, is played many important
Physiological function.Although the backbone structure of polysaccharide is simultaneously uncomplicated, with regard to degree, sulfate and two species diversity to isomery alditol
For distribution in sour chain again, the inhomogeneity of height is presented.The fine structure of chondroitin sulfate decides the specificity of function
With the interaction with multiple proteins molecule.
In terms of the selection of material, collagen and chondroitin sulfate are the organizational project biology material of function admirable
Material, biocompatibility is good, degradable absorption.From the point of view of the configuration aspects of material, the film or sponge material of single layer, mechanical property
Suitability etc. of capable of and degrading be there is a problem that certain.
Summary of the invention
The object of the present invention is to provide a kind of oral biological film preparation methods, to solve the above-mentioned deficiency of the prior art.
The purpose of the present invention is be achieved through the following technical solutions:
The present invention provides a kind of oral biological film preparation method, which includes the following steps:
I-type collagen is added in the acetic acid solution of 0.2%-06% and is configured to collagen acetic acid swelling solution；
Chondroitin sulfate is added in configured collagen acetic acid swelling solution, stirring is configured to collagen-chondroitin sulfate slurry
Liquid；
The collagen stirred evenly-chondroitin sulfate slurries are poured into stainless steel lyophilized plate, vacuum freeze drier is placed in
Middle progress first time vacuum freeze drying；
It is thin that collagen after first time vacuum freeze drying-chondroitin sulfate slurries using film laminator are pressed into collagen composite
Film；
One layer of collagen-chondroitin sulfate slurries are sprayed in two layers of collagen composite film, are put into vacuum freeze drier
Second of vacuum freeze drying of interior progress；
Collagen composite film after second of vacuum freeze drying is subjected to high-temperature vacuum crosslinking；
Collagen composite film after crosslinking is subjected to ethylene oxide sterilizing, it is raw to obtain the compound oral cavity of collagen-chondroitin sulfate
Object film.
Preferably, the I-type collagen is the I-type collagen extracted from ox heel string, is being added to 0.2%-
When in 0.6% acetic acid solution, I-type collagen mass fraction is controlled between 0.3%-0.8%, in 15 DEG C of -25 DEG C of temperature
Under with 16000r-20000r high-speed stirred per minute, be configured to collagen acetic acid swelling solution.
Preferably, when chondroitin sulfate being added in the configured collagen acetic acid swelling solution, chondroitin sulfate is controlled
Mass fraction between 0.01%-0.05%, with 16000r-20000r high-speed stirred per minute at 10 DEG C -20 DEG C, be configured to
Collagen-chondroitin sulfate slurries.
Preferably, when pouring into the collagen stirred evenly-chondroitin sulfate slurries in stainless steel lyophilized plate, after pouring into
With a thickness of 5mm ± 0.5mm.
Preferably, when collagen-chondroitin sulfate slurries is carried out vacuum freeze drying, in such a way that gradient is cooling into
Row cooling.
Preferably, the collagen composite film being pressed into through film laminator with a thickness of 1-3mm.
Preferably, the high-temperature vacuum crosslinking is the progress high-temperature vacuum friendship in 90 DEG C -110 DEG C of high-temperature vacuum drying box
Connection.
Preferably, the crosslinking method is physical crosslinking, crosslinking time 24-36h.
The invention has the benefit that (1) present invention uses ox heel string for substrate, by removal of impurities, cleaning, acid-alkali salt and life
Object processing, the high purity collagen of extraction are made, and have good histocompatbility, are suitable for that controllable degradation absorbs
Property, low antigenicity, good plasticity and mechanical property.
(2) collagen by the way of vacuum freeze drying twice-chondroitin sulfate composite membrane is the membranaceous material of dense thin
Material has good mechanical property, is convenient for clinical manipulation and requirement.
(3) after for department of stomatology operation, three-dimensional porous structure provides tissue engineering bracket for the reparation of injury tissue,
Be conducive to intrusion and the differential growth of cell, can induce the reparative regeneration of defective tissue, specific thickness can effectively hinder
Only soft tissue is grown into.
Detailed description of the invention
Below according to attached drawing, invention is further described in detail.
Fig. 1 is the flow chart of oral biological film preparation method described in the embodiment of the present invention.
Specific embodiment
As shown in Figure 1, a kind of oral biological film preparation method, the preparation method described in the embodiment of the present invention include following
Step:
The swelling of collagen acetic acid is configured to Step 1: I-type collagen is added in the acetic acid solution of 0.2%-0.6%
Liquid；
Specifically, the I-type collagen is the I-type collagen extracted from ox heel string, it is being added to 0.2%-
When in 0.6% acetic acid solution, I-type collagen mass fraction is controlled between 0.3%-0.8%, in 15 DEG C of -25 DEG C of temperature
Under with 16000r-20000r high-speed stirred per minute, be configured to collagen acetic acid swelling solution.
Step 2: chondroitin sulfate is added in configured collagen acetic acid swelling solution, it is soft that stirring is configured to collagen-sulfuric acid
Ossein slurries；
Specifically, controlling chondroitin sulfate when chondroitin sulfate being added in the configured collagen acetic acid swelling solution
Mass fraction between 0.01%-0.05%, with 16000r-20000r high-speed stirred per minute at 10 DEG C -20 DEG C, be configured to
Collagen-chondroitin sulfate slurries.
Step 3: the collagen stirred evenly-chondroitin sulfate slurries are poured into stainless steel lyophilized plate, it is placed in vacuum refrigeration
First time vacuum freeze drying is carried out in drying machine；
Specifically, when pouring into the collagen stirred evenly-chondroitin sulfate slurries in stainless steel lyophilized plate, after pouring into
With a thickness of 5mm ± 0.5mm.When collagen-chondroitin sulfate slurries are carried out vacuum freeze drying, in such a way that gradient is cooling
It is cooled down.
Step 4: the collagen after first time vacuum freeze drying-chondroitin sulfate slurries are suppressed plastic using film laminator
Former laminated film；
Specifically, the collagen composite film being pressed into through film laminator with a thickness of 1-3mm.
Step 5: spraying one layer of collagen-chondroitin sulfate slurries in two layers of collagen composite film, it is put into vacuum refrigeration
Second of vacuum freeze drying is carried out in drying machine；
Step 6: the collagen composite film after second of vacuum freeze drying is carried out high-temperature vacuum crosslinking；
Specifically, the high-temperature vacuum crosslinking is the progress high-temperature vacuum friendship in 90 DEG C -110 DEG C of high-temperature vacuum drying box
Connection.The crosslinking method is physical crosslinking, crosslinking time 24-36h.
Step 7: the collagen composite film after crosslinking is carried out ethylene oxide sterilizing, it is compound to obtain collagen-chondroitin sulfate
Oral biological film.
Detailed solution is carried out to oral biological film preparation method provided in an embodiment of the present invention with specific embodiment below
It releases.
Embodiment 1
1. prepared by slurries
1.1 raw materials thaw:
The I-type collagen extracted from ox heel string is taken out from freezing chamber, is thawed.
The preparation of 1.2 acetic acid solutions:
It takes 2-6ml acetic acid to 1000ml conical flask to add sterile water to 1000ml, is uniformly mixed
The preparation of 1.3 slurries:
I-type collagen after weighing appropriate thaw, is added in the above-mentioned acetic acid solution of 973ml, according to I-type collagen
Water content calculates I-type collagen dosage, and control I-type collagen mass fraction is 0.3%, stirs 1-2 hours, forms collagen
Acetic acid swelling solution.
It weighs 0.10g chondroitin sulfate to be added in the above-mentioned acetic acid solution of 27ml, with 0.22 μm of filter after stirring
Film filtration sterilization is slowly added to using peristaltic pump into the collagen acetic acid swelling solution stirred evenly, and collagen-sulfuric acid is formed
Chondroitin slurries.
2 air extract
Stirring rear slurry is added in bottle,suction, vacuum pump jog bottle,suction frequently is opened, is evacuated to bubble-free in slurries.
3 freeze-dryings
3.1 pour into the collagen for being evacuated de-bubble-chondroitin sulfate slurries 250ml in stainless steel lyophilized plate, the thickness after pouring into
Degree be 5mm ± 0.5mm vacuumized by pre-freeze, gradient freezing heating and etc. be freeze-dried.
3.2 are compressed to the collagen after freeze-drying-chondroitin sulfate sponge using film laminator the collagen composite of thickness 1mm
Film.
After uniformly spraying slurries between 3.3 two layers of collagen composite film, two panels film is pasted together, is carried out second
Vacuum freeze drying.
The crosslinking of 4 vacuum high-temperatures
Laminated film after second of vacuum freeze drying is wrapped up into one layer of aluminium-foil paper, is placed in high-temperature vacuum drying box,
The lower 90 DEG C of crosslinkings 36h of vacuum environment.
5 ethylene oxide sterilizings
Film after crosslinking is cut into different size, after double casing, utilizes ethylene oxide sterilizing.
Embodiment 2
The preparation method of oral biological film of the invention, system the following steps are included:
1. prepared by slurries
1.1 raw materials thaw:
I-type collagen is taken out from freezing chamber, thaws.
The preparation of 1.2 acetic acid solutions:
It takes 2-6ml acetic acid to 1000ml conical flask to add sterile water to 1000ml, is uniformly mixed；
The preparation of 1.3 slurries:
I-type collagen after weighing appropriate thaw, is added in the above-mentioned acetic acid solution of 865ml, according to I-type collagen
Water content calculates I-type collagen dosage, and control I-type collagen mass fraction is 0.8%, stirs 1-2 hours, forms collagen
Acetic acid swelling solution.
It weighs 0.5g chondroitin sulfate to be added in the above-mentioned acetic acid solution of 135ml, with 0.22 μm of filter after stirring
Film filtration sterilization is slowly added to using peristaltic pump into the collagen acetic acid swelling solution stirred evenly, and collagen-sulfuric acid is formed
Chondroitin slurries.
2 air extract
Stirring rear slurry is added in bottle,suction, vacuum pump jog bottle,suction frequently is opened, is evacuated to bubble-free in slurries.
3 freeze-dryings
3.1 pour into the slurries 250ml for being evacuated de-bubble in stainless steel lyophilized plate, after pouring into a thickness of 5mm ± 0.5mm,
By pre-freeze, vacuumize, gradient freezing heating and etc. be freeze-dried.
3.2 are compressed to the collagen after freeze-drying-chondroitin sulfate sponge using film laminator the type i collagen of thickness 2mm
Protein film.
After uniformly spraying slurries between 3.3 two layers of I-type collagen film, two panels film is pasted together, secondary freezing
It is dry.
The crosslinking of 4 vacuum high-temperatures
Laminated film after secondary freeze drying is wrapped up into one layer of aluminium-foil paper, is placed in high-temperature vacuum drying box, vacuum ring
The lower 110 DEG C of crosslinkings in border are for 24 hours.
5 ethylene oxide sterilizings
Film after crosslinking is cut into different size, after double casing, utilizes ethylene oxide sterilizing.
The preparation method of the oral biological film provided by above-mentioned specific embodiment can be quickly obtained oral biological film,
The oral biological film that the present invention produces has the advantage that (1) uses ox heel string for substrate, by removal of impurities, cleaning, acid-alkali salt
And biological processes, the high purity collagen of extraction are made, and have good histocompatbility, are suitable for controllable degradation
Absorbability, low antigenicity, good plasticity and mechanical property.
(2) collagen by the way of vacuum freeze drying twice-chondroitin sulfate composite membrane is the membranaceous material of dense thin
Material has good mechanical property, is convenient for clinical manipulation and requirement.
(3) after for department of stomatology operation, three-dimensional porous structure provides tissue engineering bracket for the reparation of injury tissue,
Be conducive to intrusion and the differential growth of cell, can induce the reparative regeneration of defective tissue, specific thickness can effectively hinder
Only soft tissue is grown into.
The present invention is not limited to above-mentioned preferred forms, anyone can show that other are various under the inspiration of the present invention
The product of form, however, make any variation in its shape or structure, it is all that there is skill identical or similar to the present application
Art scheme, is within the scope of the present invention.
Claims (7)
1. a kind of oral biological film preparation method, which is characterized in that include the following steps:
I-type collagen is added in the acetic acid solution of 0.2%-0.6% and is configured to collagen acetic acid swelling solution；The I type glue
Former albumen is the I-type collagen extracted from ox heel string, when in the acetic acid solution for being added to 0.2%-0.6%, controls I type
Collagen quality score is between 0.3%-0.8%, with 16000r-20000r high speed per minute at a temperature of 15 DEG C -25 DEG C
Stirring, is configured to collagen acetic acid swelling solution；
Chondroitin sulfate is added in configured collagen acetic acid swelling solution, stirring is configured to collagen-chondroitin sulfate slurries；In
When chondroitin sulfate being added in the configured collagen acetic acid swelling solution, the mass fraction for controlling chondroitin sulfate exists
Between 0.01%-0.05%, with 16000r-20000r high-speed stirred per minute at 10 DEG C -20 DEG C, it is soft to be configured to collagen-sulfuric acid
Ossein slurries；
The collagen stirred evenly-chondroitin sulfate slurries are poured into stainless steel lyophilized plate, be placed in vacuum freeze drier into
Row first time vacuum freeze drying；
Collagen after first time vacuum freeze drying-chondroitin sulfate slurries are pressed into collagen composite film using film laminator；
One layer of collagen-chondroitin sulfate slurries are sprayed in two layers of collagen composite film, are put into vacuum freeze drier and are carried out the
Secondary vacuum freeze-drying；
Collagen composite film after second of vacuum freeze drying is subjected to high-temperature vacuum crosslinking；
Collagen composite film after crosslinking is subjected to ethylene oxide sterilizing, obtains the compound oral biological film of collagen-chondroitin sulfate.
2. oral biological film preparation method according to claim 1, which is characterized in that in the collagen-sulphur that will be stirred evenly
When aching and limp ossein slurries are poured into stainless steel lyophilized plate, after pouring into a thickness of 5mm ± 0.5mm.
3. oral biological film preparation method according to claim 1 or 2, which is characterized in that by collagen-chondroitin sulfate
When slurries carry out vacuum freeze drying, cooled down in such a way that gradient is cooling.
4. oral biological film preparation method according to claim 1 or 2, which is characterized in that described to be pressed into through film laminator
Collagen composite film with a thickness of 1-3mm.
5. oral biological film preparation method according to claim 1 or 2, which is characterized in that the high-temperature vacuum, which is crosslinked, is
High-temperature vacuum crosslinking is carried out in 90 DEG C -110 DEG C of high-temperature vacuum drying box.
6. the preparation method of oral biological film according to claim 1 or 2, which is characterized in that the crosslinking method is
Physical crosslinking, crosslinking time 24-36h.
7. the oral biological film that any one of -6 the methods prepare according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410101451.4A CN104922732B (en) | 2014-03-19 | 2014-03-19 | A kind of oral biological film preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410101451.4A CN104922732B (en) | 2014-03-19 | 2014-03-19 | A kind of oral biological film preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104922732A CN104922732A (en) | 2015-09-23 |
| CN104922732B true CN104922732B (en) | 2019-11-15 |
Family
ID=54110371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410101451.4A Active CN104922732B (en) | 2014-03-19 | 2014-03-19 | A kind of oral biological film preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104922732B (en) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105381504B (en) * | 2015-11-17 | 2018-08-21 | 浙江星月生物科技股份有限公司 | A kind of collagen-based cartilage frame |
| EP3175869A1 (en) * | 2015-12-04 | 2017-06-07 | Geistlich Pharma AG | Resorbable crosslinked formstable membrane |
| CN105688288B (en) * | 2016-03-09 | 2019-03-05 | 四川大学 | Compound oral restoration membrane material of collagen-based and preparation method thereof |
| CN107050519B (en) * | 2016-12-22 | 2021-04-13 | 天新福（北京）医疗器材股份有限公司 | Preparation method of multilayer absorbable biological membrane |
| CN107050521B (en) * | 2017-04-27 | 2020-10-09 | 天新福（北京）医疗器材股份有限公司 | Double-layer collagen dermal scaffold and preparation method thereof |
| CN109954167B (en) * | 2019-02-28 | 2021-09-21 | 天新福（北京）医疗器材股份有限公司 | Bone repair material and application thereof |
| CN110075363A (en) * | 2019-05-07 | 2019-08-02 | 江苏地韵医疗科技有限公司 | One kind having omnidirectional's through-hole structure collagen scaffold and preparation method thereof |
| CN110368525B (en) * | 2019-09-03 | 2021-11-23 | 山东奥精生物科技有限公司 | Oral cavity film for reconstruction of attached gingiva and preparation method thereof |
| CN110559479B (en) * | 2019-09-12 | 2021-12-07 | 苏州高哲斯环境新材料有限公司 | Preparation method of tissue regeneration guiding membrane for tooth restoration |
| CN110743044B (en) * | 2019-11-23 | 2022-03-08 | 北京银河巴马生物技术股份有限公司 | Dental bone-guided regenerated collagen membrane and preparation method thereof |
| CN114177358B (en) * | 2021-12-13 | 2022-07-19 | 天新福（北京）医疗器材股份有限公司 | Biological scaffold, and forming method and application thereof |
| CN114259324B (en) * | 2021-12-24 | 2022-09-30 | 天新福（北京）医疗器材股份有限公司 | Preparation method of multilayer artificial dura mater |
| CN114272440B (en) * | 2021-12-24 | 2022-08-19 | 天新福（北京）医疗器材股份有限公司 | Preparation method of biological membrane for skull base reconstruction operation |
| CN114058068B (en) * | 2022-01-17 | 2022-04-12 | 天新福（北京）医疗器材股份有限公司 | Tissue-guided regenerated collagen membrane and preparation method thereof |
| CN114053480B (en) * | 2022-01-17 | 2022-05-03 | 天新福（北京）医疗器材股份有限公司 | Soft tissue repair collagen membrane and preparation method thereof |
| CN118304483A (en) * | 2023-01-06 | 2024-07-09 | 陕西慧康生物科技有限责任公司 | Natural double-layer recombinant collagen-like protein absorbable biological membrane and preparation method and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1255479C (en) * | 2003-09-23 | 2006-05-10 | 中国医学科学院生物医学工程研究所 | Composite bone tissue engineering rack material and its prepn |
| DE102009025190A1 (en) * | 2009-06-12 | 2010-12-16 | Call, Krimhild | Enzyme-based method for coupling- and/or crosslinking reactions of e.g. textiles, comprises coating surfaces of products, modifying suitable compounds, and coupling and/or crosslinking suitable compounds as coupling agents to the compounds |
| CN103191085B (en) * | 2012-01-09 | 2016-08-03 | 深圳兰度生物材料有限公司 | A kind of two-layer compound regeneration membrane and preparation method thereof |
-
2014
- 2014-03-19 CN CN201410101451.4A patent/CN104922732B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104922732A (en) | 2015-09-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104922732B (en) | A kind of oral biological film preparation method | |
| Zhang et al. | Layered nanofiber sponge with an improved capacity for promoting blood coagulation and wound healing | |
| CN108404209B (en) | Collagen-fibroin co-assembled sponge material, co-assembled artificial skin and preparation method thereof | |
| Cui et al. | Collagen-tussah silk fibroin hybrid scaffolds loaded with bone mesenchymal stem cells promote skin wound repair in rats | |
| CA2673589C (en) | Method of drying biological material | |
| CN102743796B (en) | Silk fibroin porous support made from polyvinyl alcohol, and preparation method and application thereof | |
| CN104707183B (en) | Absorbable orthopaedics internal fixation material based on silk-fibroin(s) and preparation method thereof | |
| CN110743044B (en) | Dental bone-guided regenerated collagen membrane and preparation method thereof | |
| JP2017506097A (en) | Compartmented cryopreservation container and use thereof | |
| CN102861360B (en) | Nerve repair promoting material and preparation method and application thereof | |
| CN106581776A (en) | Directional microporous collagen/chitosan/silk fibroin composite scaffold and preparation method thereof | |
| Been et al. | Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration | |
| CN107126579A (en) | A kind of former protein sponge of Yak-skin Gelatin acted on quick-acting haemostatic powder and its preparation method and application | |
| CN1259980C (en) | Biologic material for medical use and its preparing process and usage | |
| CN105037752A (en) | Modified polyvinyl alcohol cornea repair material with collagen in graded distribution and preparation method | |
| US20170368231A1 (en) | Bioengineered Regenerative Graft Matrix, and Methods for Making Thereof | |
| CN108409992A (en) | Fibroin albumen bulk and preparation method thereof | |
| CN104587525A (en) | Scaffold containing platelets and hyaluronic acid and preparation method of scaffold | |
| CN106039400A (en) | Method using ice crystal template method to prepare three-dimensional biological scaffold with regular lamellar structure and application of method | |
| CN107715166A (en) | Load preparation method of composite nano fiber dressing of mescenchymal stem cell and products thereof and application | |
| WO2011050622A1 (en) | Granular biomaterial for tissue repairing and preparation method thereof | |
| CN107519535A (en) | Degradable sodium alginate/fibroin bilayer skin tissue engineering bracket material and preparation method thereof | |
| CN106386787A (en) | Breast cancer stem cell cryopreservation protection agent and prepared cryopreservation protection kit | |
| Ding et al. | Dual green hemostatic sponges constructed by collagen fibers disintegrated from Halocynthia roretzi by a shortcut method | |
| CN109867811B (en) | Porous-solid composite silk material and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| CB02 | Change of applicant information |
Address after: 102200 Changping District science and Technology Park, Beijing torch street, No. 30 Applicant after: Tianxinfu (Beijing) medical equipment Limited by Share Ltd Address before: 102200 Changping District science and Technology Park, Beijing torch street, No. 30 Applicant before: Beijing Tian Xin Fu Medical Appliance Co., Ltd. |
|
| COR | Change of bibliographic data | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |