CN105287709B - A kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect - Google Patents
A kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect Download PDFInfo
- Publication number
- CN105287709B CN105287709B CN201510686098.5A CN201510686098A CN105287709B CN 105287709 B CN105287709 B CN 105287709B CN 201510686098 A CN201510686098 A CN 201510686098A CN 105287709 B CN105287709 B CN 105287709B
- Authority
- CN
- China
- Prior art keywords
- volume
- hickory leaves
- total flavone
- pinostrobin
- hickory
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000723418 Carya Species 0.000 title claims abstract description 51
- 229930003944 flavone Natural products 0.000 title claims abstract description 32
- 235000011949 flavones Nutrition 0.000 title claims abstract description 32
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 31
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 31
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 31
- 230000000694 effects Effects 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- RTIXKCRFFJGDFG-UHFFFAOYSA-N chrysin Chemical compound C=1C(O)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=CC=C1 RTIXKCRFFJGDFG-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000003814 drug Substances 0.000 claims abstract description 17
- ORJDDOBAOGKRJV-CQSZACIVSA-N (2S)-Pinocembrin Natural products C1([C@H]2CC(=O)C3=C(O)C=C(C=C3O2)OC)=CC=CC=C1 ORJDDOBAOGKRJV-CQSZACIVSA-N 0.000 claims abstract description 14
- ORJDDOBAOGKRJV-UHFFFAOYSA-N Dihydrotectochrysin Natural products O1C2=CC(OC)=CC(O)=C2C(=O)CC1C1=CC=CC=C1 ORJDDOBAOGKRJV-UHFFFAOYSA-N 0.000 claims abstract description 14
- CUGDOWNTXKLQMD-BQYQJAHWSA-N Pinostrobin chalcone Chemical compound OC1=CC(OC)=CC(O)=C1C(=O)\C=C\C1=CC=CC=C1 CUGDOWNTXKLQMD-BQYQJAHWSA-N 0.000 claims abstract description 14
- ORJDDOBAOGKRJV-AWEZNQCLSA-N pinostrobin Chemical compound C1([C@@H]2CC(=O)C3=C(O)C=C(C=C3O2)OC)=CC=CC=C1 ORJDDOBAOGKRJV-AWEZNQCLSA-N 0.000 claims abstract description 14
- 201000001431 Hyperuricemia Diseases 0.000 claims abstract description 10
- NYCXYKOXLNBYID-UHFFFAOYSA-N 5,7-Dihydroxychromone Natural products O1C=CC(=O)C=2C1=CC(O)=CC=2O NYCXYKOXLNBYID-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000015838 chrysin Nutrition 0.000 claims abstract description 9
- 229940043370 chrysin Drugs 0.000 claims abstract description 9
- CUGDOWNTXKLQMD-UHFFFAOYSA-N Pinostrobinchalkon Natural products OC1=CC(OC)=CC(O)=C1C(=O)C=CC1=CC=CC=C1 CUGDOWNTXKLQMD-UHFFFAOYSA-N 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 68
- 235000019441 ethanol Nutrition 0.000 claims description 30
- 238000010828 elution Methods 0.000 claims description 21
- 239000000706 filtrate Substances 0.000 claims description 16
- 238000011068 loading method Methods 0.000 claims description 12
- 239000011347 resin Substances 0.000 claims description 10
- 229920005989 resin Polymers 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 238000010936 aqueous wash Methods 0.000 claims description 6
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 238000004821 distillation Methods 0.000 claims description 4
- 150000002576 ketones Chemical class 0.000 claims description 4
- 238000000643 oven drying Methods 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 4
- NYSZJNUIVUBQMM-BQYQJAHWSA-N Cardamonin Chemical compound COC1=CC(O)=CC(O)=C1C(=O)\C=C\C1=CC=CC=C1 NYSZJNUIVUBQMM-BQYQJAHWSA-N 0.000 claims 6
- NYSZJNUIVUBQMM-UHFFFAOYSA-N alpinetin chalcone Natural products COC1=CC(O)=CC(O)=C1C(=O)C=CC1=CC=CC=C1 NYSZJNUIVUBQMM-UHFFFAOYSA-N 0.000 claims 3
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 abstract description 16
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 abstract description 16
- 229940116269 uric acid Drugs 0.000 abstract description 16
- 229940079593 drug Drugs 0.000 abstract description 12
- 210000002966 serum Anatomy 0.000 abstract description 8
- 240000001417 Vigna umbellata Species 0.000 abstract description 7
- 235000011453 Vigna umbellata Nutrition 0.000 abstract description 7
- 238000011552 rat model Methods 0.000 abstract description 3
- 230000036541 health Effects 0.000 abstract description 2
- 239000000284 extract Substances 0.000 description 8
- 241000700159 Rattus Species 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- 108010093894 Xanthine oxidase Proteins 0.000 description 2
- 102100033220 Xanthine oxidase Human genes 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 2
- OFCNXPDARWKPPY-UHFFFAOYSA-N allopurinol Chemical compound OC1=NC=NC2=C1C=NN2 OFCNXPDARWKPPY-UHFFFAOYSA-N 0.000 description 2
- 229960003459 allopurinol Drugs 0.000 description 2
- 230000002961 anti-hyperuricemic effect Effects 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- WHQCHUCQKNIQEC-UHFFFAOYSA-N benzbromarone Chemical compound CCC=1OC2=CC=CC=C2C=1C(=O)C1=CC(Br)=C(O)C(Br)=C1 WHQCHUCQKNIQEC-UHFFFAOYSA-N 0.000 description 2
- 229960002529 benzbromarone Drugs 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000006837 decompression Effects 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000002960 lipid emulsion Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000003424 uricosuric effect Effects 0.000 description 2
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 1
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 1
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 240000004875 Carya cathayensis Species 0.000 description 1
- 235000005663 Carya cathayensis Nutrition 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 201000005569 Gout Diseases 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 244000264601 Juglans mandschurica Species 0.000 description 1
- 235000014075 Juglans mandschurica Nutrition 0.000 description 1
- 208000000913 Kidney Calculi Diseases 0.000 description 1
- 208000001145 Metabolic Syndrome Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010029148 Nephrolithiasis Diseases 0.000 description 1
- 241000899834 Obovaria olivaria Species 0.000 description 1
- 241000168036 Populus alba Species 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 201000000690 abdominal obesity-metabolic syndrome Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003527 anti-angiogenesis Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000003405 delayed action preparation Substances 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- -1 oral solution Substances 0.000 description 1
- 229940100688 oral solution Drugs 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 238000005220 pharmaceutical analysis Methods 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000004144 purine metabolism Effects 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008085 renal dysfunction Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000007901 soft capsule Substances 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect, the Hickory Leaves extractive total flavone contain pinostrobin, pinostrobin chalcone, Chrysin and the bright gross mass percentage of red bean bandit 50% or more.The present invention is to rely on Hickory Leaves, prepares the general flavone in Hickory Leaves, develops its effect, expands the new medicine resource of Hickory Leaves.The Hickory Leaves extractive total flavone can significantly reduce the serum uric acid level of rat model, can be used for preventing and treating drug and health product that hyperuricemia is major function.
Description
Technical field
The present invention relates to pharmaceutical technology field, in particular to a kind of Hickory Leaves extract with anti-trioxypurine effect is always yellow
Ketone as well as preparation method and application thereof.
Background technique
Uric acid is the metabolite of purine in endogenous purine and diet.In the purine metabolism of human body, ATP etc. passes through one
The metabolic response of series, produces hypoxanthine and xanthine, and the two generates uric acid under xanthine oxidase effect.When purine generation
When thanking to disorder, serum uric acid level is excessively high, leads to the generation of hyperuricemia (Hyperuricemia), then uric acid is caused to be saltoutd
Joint is crystallized and be deposited in out, gout is caused to be broken out, and is often involved kidney and is caused arteriosclerotic kidney and uric acid kidney stones, and
And it may cause Metabolic syndrome and seek peace relevant cardiovascular disease.In recent years, the disease incidence of hyperuricemia sharply increases, and has surpassed
10% is crossed, one of big chronic disease of 21 century 20 is classified as by the United Nations.
Prevention and treatment hyperuricemia class drug is broadly divided into uric acid and generates depressant (such as Allopurinol) and uricosuric excretion at present
Two kinds of medicine (such as Benzbromarone), although these drugs have apparent anti-trioxypurine to act on, side effect is also larger, such as fash, hair
Heat, hepatic renal dysfunction etc., thus the range limited its application.Therefore, developmental research drug effect is clear and the novel of safety resists
Antihyperuricemic disease drug is very necessary.According to the literature, inhibit in natural plant crude drugs source containing a large amount of xanthine oxidases
The ingredient of agent and uricosuric excretion, and small toxicity, it is highly-safe, there is preferable research and development prospect.
Chinese walnut (Carya cathayensis Sarg.) is Juglandaceae hickory plant, Hickory Leaves frequently as
Folk tradition Chinese herbal medicine, for the purposes such as clearing heat and detoxicating, existing research shows that Hickory Leaves extract contains a large amount of anti-oxidant work
Property substance, predominantly Flavonoid substances, have apparent anti-aging, antitumor, anti-angiogenesis and other effects.Currently, there is not yet
To the relevant report using Hickory Leaves extractive total flavone treatment hyperuricemia.Inventors discovered through research that hickory nut
Leaf extract pinostrobin can reduce rat model serum uric acid level, can be used for preventing and treating the drug that hyperuricemia is major function
And health food.
Summary of the invention
The purpose of the present invention is to provide a kind of Hickory Leaves extractive total flavones with anti-trioxypurine effect, provide simultaneously
Preparation method and the usage.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of Hickory Leaves extractive total flavone with anti-trioxypurine effect, contains pinostrobin, pinostrobin chalcone, white poplar
Plain and bright red bean bandit gross mass percentage is 50% or more.
A kind of preparation method of the Hickory Leaves extractive total flavone with anti-trioxypurine effect, comprising the following steps:
(1) 35-65 DEG C of oven drying of Hickory Leaves is taken, 40-80 mesh is crushed to, is put into round-bottomed flask, volume hundred is added
Dividing concentration is 70-95% ethanol water, is extracted 20-100 minutes in 80-110 DEG C of normal pressure, repeats to extract 1-3 times；Then with filter
Paper filtering, merging filtrate；
(2) it is 45-65% that filtrate is concentrated under reduced pressure into filtrate concentration of alcohol under 300 ± 100pa vacuum degree, in centrifuging and taking
This supernatant is crossed SD200 large pore resin absorption column (filling SD200 aromatic series macroporous absorbent resin) by clear liquid, and loading speed is
1BV/h-8BV/h, loading volume 4-8BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocity, with distillation water elution tree
Rouge, elution volume 5-10BV；It is 10- with concentration expressed in percentage by volume with 1BV/h-8BV/h flow velocity under 20-35 DEG C, normal pressure
30% ethanol aqueous wash deresination, elution volume 8-12BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocity,
The ethanol aqueous wash deresination for being 40-90% with concentration expressed in percentage by volume, elution volume 8-12BV；Collect 40-90% ethyl alcohol
Eluent, concentration recycle ethyl alcohol, are dried under reduced pressure, obtain Hickory Leaves extractive total flavone, wherein pinostrobin, pinostrobin Cha Er
Ketone, Chrysin and the bright total content of red bean bandit are 50% or more.
Preferably, 70-95% ethanol water dosage is that every g Hickory Leaves crushed material adds 10mL in step (1).
Preferably, parameter of noncentricity in step (2) are as follows: 2000-4000 rpms, be centrifuged 20 minutes.With anti-trioxypurine
The Hickory Leaves extractive total flavone of effect is preparing the purposes in antihyperuricemic disease drug.
Above-mentioned Hickory Leaves extractive total flavone can with the auxiliary material approved on medicament or food, using Modern preparations skill
Capsule, tablet, powder, oral solution, soft capsule, pill, gel based on Hickory Leaves extractive total flavone is made in art
The various dosage forms such as agent, sustained release preparation.
The beneficial effects of the present invention are: the present invention is to rely on Hickory Leaves, the general flavone in Hickory Leaves is prepared, is developed
Its effect expands the new medicine resource of Hickory Leaves.The experiment of inventor shows: the Hickory Leaves extractive total flavone can be shown
The serum uric acid level for reducing rat model is write, can be used for preventing and treating drug and health product that hyperuricemia is major function.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.
In the present invention, if not refering in particular to, used raw material and equipment etc. are commercially available or commonly used in the art.
Method in following embodiments is unless otherwise instructed the conventional method of this field.
Embodiment
A kind of preparation method of the Hickory Leaves extractive total flavone with anti-trioxypurine effect, comprising the following steps: (1) take
35-65 DEG C of oven drying of Hickory Leaves, is crushed to 40-80 mesh, is put into round-bottomed flask, and addition concentration expressed in percentage by volume is 70-
95% ethanol water (every g Hickory Leaves crushed material adds 10mL) extracts 20-100 minutes in 80-110 DEG C of normal pressure, repeats to extract
1-3 times；Then it is filtered with filter paper, merging filtrate；
(2) filtrate is concentrated under reduced pressure under 300 ± 100pa vacuum degree, makes concentration of alcohol 45-65% in filtrate, centrifugation
(2000-4000 rpms, be centrifuged 20 minutes) takes supernatant, this supernatant is crossed SD200 large pore resin absorption column, loading speed
Degree is 1BV/h-8BV/h, loading volume 4-8BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocity, washed with distillation
Deresination, elution volume 5-10BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocity, it is with concentration expressed in percentage by volume
The ethanol aqueous wash deresination of 10-30%, elution volume 8-12BV；Under 20-35 DEG C, normal pressure, flowed with 1BV/h-8BV/h
Speed, the ethanol aqueous wash deresination for being 40-90% with concentration expressed in percentage by volume, elution volume 8-12BV；Collect 40-90% second
Alcohol eluen, concentration recycle ethyl alcohol, are dried under reduced pressure, obtain Hickory Leaves extractive total flavone, wherein pinostrobin, pinostrobin are looked into
Your ketone, Chrysin and the bright total content of red bean bandit are 50% or more.
Specific embodiment 1
After Hickory Leaves are dry, it is mechanically pulverized, weighs Hickory Leaves powder 200g, add the volume basis of 10 times of amounts (g:mL)
Than the ethanol solution for 95%, extract 2 times, each 30min, merging filtrate is concentrated under reduced pressure under 300 ± 100pa vacuum degree, makes
Concentration of alcohol is 55% in filtrate, 3000 rpms, is centrifuged 20 minutes, takes supernatant.Weigh SD200 aromatic series macroporous absorption tree
Rouge (Zhejiang Province Zhengguan Industry Co., Ltd) 4.0g is put into chromatographic column (15mm × 350mm i.d), with the flow velocity of 2BV/h
Carry out loading, loading volume 6.5BV.10BV water elution is first used, then is washed with the ethanol solution that 10BV percent by volume is 20%
It is de-, it is finally eluted with the ethanol solution that 10BV percent by volume is 70%, elution speed 4BV/h collects 70% ethanol elution
Liquid, decompression rotary evaporated to dryness is dry, obtains Hickory Leaves extractive total flavone, wherein pinostrobin, pinostrobin chalcone, Chrysin
And the bright total content of red bean bandit is 55.3% or more.
Specific embodiment 2
It after Hickory Leaves are dry, are mechanically pulverized, weigh Hickory Leaves powder 400g, adding 10 times of amount percents by volume is 95%
Ethanol solution, extract 2 times, each 30min, merging filtrate is concentrated under reduced pressure under 300 ± 100pa vacuum degree, makes second in filtrate
Determining alcohol is 55%, 3000 rpms, is centrifuged 20 minutes, takes supernatant.SD200 macroporous absorbent resin 200.0g is weighed, is put into
In chromatographic column (15mm × 350mm i.d), loading, loading volume 6.5BV are carried out with the flow velocity of 2BV/h.First washed with 10BV
It is de-, then the ethanol solution elution for being 20% with 10BV percent by volume, it is finally molten for 70% ethyl alcohol with 10BV percent by volume
Liquid elution, elution speed 4BV/h collect 70% ethanol eluate, and decompression rotary evaporated to dryness is dry, obtain Hickory Leaves and extract
Object general flavone, wherein the bright total content of pinostrobin, pinostrobin chalcone, Chrysin, red bean bandit is 52.2% or more.
The bright total content measuring method of pinostrobin, pinostrobin chalcone, Chrysin, red bean bandit is referring to the efficient liquid of the such as Shen Yong
Phase chromatography measures 5 flavone aglycone content [J] Pharmaceutical Analysis magazines in Hickory Leaves, 05 phase in 2013.
Effect test
Hickory Leaves extractive total flavone finds the hyperuricemia model rat prepared to fat emulsion through long term administration
Serum uric acid level have reduce effect significantly.
1 experimental material and instrument
1.1 materials and instrument
1.1 animal SD rats, male, weight (200-500g), 60.Quality certification number: scxk (Zhejiang) 20080033.
1.2 drugs are prepared
1.2.1 test drug
Hickory Leaves extractive total flavone, being configured to concentration with 0.3%CMC-Na is 200mg/kg and 100.0mg/kg.
1.2.2 positive drug allopurinol tablet, specification 100mg × 200 piece, Jiangsu side force pharmaceutical factory Co., Ltd, 10mg/
Kg and benzbromarone tablets, specification 50mg × 10 piece, the German pharmaceutical factory He Man great, 5mg/kg.
1.3 instrument
Electronic balance CP114 (Shanghai Ao Haosi Instrument Ltd.)；Electric jacket (the limited public affairs of the bold and unconstrained laboratory apparatus of Nantong benefit
Department)；GG17 Rotary Evaporators (Shensheng Science & Tech. Co., Ltd., Shanghai)；SZ-93A dual pure water distiller (Shanghai Asia Rong Sheng automatically
Change instrument plant)；Refrigerator (Qingdao HaiEr Co., Ltd)；Dionex high performance liquid chromatography (Dai An company, the U.S.)；TBA-
AOFR (ACCUTE) automatic clinical chemistry analyzer (Japanese wide medical Co., Ltd., Toshiba three).
2 experimental methods
2.1 administration
60 rats are randomly divided into normal group according to weight, model control group, positive controls, Hickory Leaves extract
General flavone high dose group (200mg/kg), Hickory Leaves extractive total flavone low dose group (100mg/kg), normal group give it is pure
Change water, model group gives fat emulsion.Tap water is given in gastric infusion, model group and normal stock stomach-filling, and administration group gives correspondence
Drug, administration capacity are 1mL/100g weight.
2.2 observation index
Successive administration 8 weeks, take hematometry rat blood serum uric acid (UA) horizontal every 2 weeks.
2.3 statistics and analysis
Measurement data data result withIt indicates, group difference uses t-test.
3 experimental results
Normal group, model group and each administration group serum uric acid situation of change during 3.1 administrations
Compared with normal group, model group rats serum uric acid is significantly raised (P < 0.01)；Compared with model group, it is administered 4 weeks
The high low dose group of Hickory Leaves extractive total flavone can be substantially reduced model group rats UA level (P < 0.05), and anti-trioxypurine effect
It is steady to continue.It the results are shown in Table 1.
The variation (X ± S, n=10) of 1 successive administration rat UA level of table
Note: compared with normal group, P < 0.01 * P < 0.05, * *；Compared with model control group, #P < 0.05, ##P < 0.01.
Above-mentioned embodiment is only a preferred solution of the present invention, not the present invention is made in any form
Limitation, there are also other variations and modifications on the premise of not exceeding the technical scheme recorded in the claims.
Claims (5)
1. a kind of Hickory Leaves extractive total flavone with anti-trioxypurine effect, it is characterised in that: looked into containing pinostrobin, pinostrobin
The gross mass percentage of your ketone, Chrysin and cardamomin is 50% or more；
The preparation of the Hickory Leaves extractive total flavone with anti-trioxypurine effect the following steps are included:
(1) 35-65 DEG C of oven drying of Hickory Leaves is taken, 40-80 mesh is crushed to, is put into round-bottomed flask, it is dense that volume basis is added
Degree is 70-95% ethanol water, is extracted 20-100 minutes in 80-110 DEG C of normal pressure, repeats to extract 1-3 times；Then with filter paper mistake
Filter, merging filtrate；
(2) it is 45-65%, centrifuging and taking supernatant that filtrate is concentrated under reduced pressure into concentration of alcohol in filtrate under 300 ± 100pa vacuum degree
This supernatant is crossed SD200 large pore resin absorption column by liquid, and loading speed is 1BV/h-8BV/h, loading volume 4-8BV；?
20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, with distillation water elution resin, elution volume 5-10BV；At 20-35 DEG C,
Under normal pressure, with 1BV/h-8BV/h flow velocity, the ethanol aqueous wash deresination for being 10-30% with concentration expressed in percentage by volume, elution volume
For 8-12BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocity, the ethyl alcohol for being 40-90% with concentration expressed in percentage by volume is water-soluble
Liquid elutes resin, elution volume 8-12BV；40-90% ethanol eluate is collected, concentration recycles ethyl alcohol, is dried under reduced pressure, obtains
Hickory Leaves extractive total flavone, wherein the total content of pinostrobin, pinostrobin chalcone, Chrysin and cardamomin 50% with
On.
2. a kind of preparation method of the Hickory Leaves extractive total flavone with anti-trioxypurine effect, which is characterized in that including following
Step:
(1) 35-65 DEG C of oven drying of Hickory Leaves is taken, 40-80 mesh is crushed to, is put into round-bottomed flask, it is dense that volume basis is added
Degree is 70-95% ethanol water, is extracted 20-100 minutes in 80-110 DEG C of normal pressure, repeats to extract 1-3 times；Then with filter paper mistake
Filter, merging filtrate；
(2) it is 45-65%, centrifuging and taking supernatant that filtrate is concentrated under reduced pressure into concentration of alcohol in filtrate under 300 ± 100pa vacuum degree
This supernatant is crossed SD200 large pore resin absorption column by liquid, and loading speed is 1BV/h-8BV/h, loading volume 4-8BV；?
20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, with distillation water elution resin, elution volume 5-10BV；At 20-35 DEG C,
Under normal pressure, with 1BV/h-8BV/h flow velocity, the ethanol aqueous wash deresination for being 10-30% with concentration expressed in percentage by volume, elution volume
For 8-12BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocity, the ethyl alcohol for being 40-90% with concentration expressed in percentage by volume is water-soluble
Liquid elutes resin, elution volume 8-12BV；40-90% ethanol eluate is collected, concentration recycles ethyl alcohol, is dried under reduced pressure, obtains
Hickory Leaves extractive total flavone, wherein the total content of pinostrobin, pinostrobin chalcone, Chrysin and cardamomin 50% with
On.
3. preparation method according to claim 2, it is characterised in that: 70-95% ethanol water dosage is in step (1)
Every g Hickory Leaves crushed material adds 10mL.
4. preparation method according to claim 2, it is characterised in that: parameter of noncentricity in step (2) are as follows: 2000-4000 turns
Per minute, it is centrifuged 20 minutes.
5. the Hickory Leaves extractive total flavone with anti-trioxypurine effect is in preparation hyperuricemia medicine as described in claim 1
Purposes in object.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510686098.5A CN105287709B (en) | 2015-10-20 | 2015-10-20 | A kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510686098.5A CN105287709B (en) | 2015-10-20 | 2015-10-20 | A kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105287709A CN105287709A (en) | 2016-02-03 |
| CN105287709B true CN105287709B (en) | 2019-09-13 |
Family
ID=55185996
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510686098.5A Active CN105287709B (en) | 2015-10-20 | 2015-10-20 | A kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105287709B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107252440A (en) * | 2017-06-05 | 2017-10-17 | 浙江中医药大学 | A kind of preparation method and purposes of the Hickory Leaves general flavone with hypoglycemic effect |
| CN107519234A (en) * | 2017-08-28 | 2017-12-29 | 付林春 | A kind of preparation technology of Hickory Leaves extractive of general flavone |
| CN111494569A (en) * | 2020-06-19 | 2020-08-07 | 陈正 | Preparation method of walnut leaf powder instant beverage for relieving gout |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142685A (en) * | 2013-02-07 | 2013-06-12 | 浙江中医药大学 | Method for extraction of total flavonoid aglycones from hickory leaves |
-
2015
- 2015-10-20 CN CN201510686098.5A patent/CN105287709B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142685A (en) * | 2013-02-07 | 2013-06-12 | 浙江中医药大学 | Method for extraction of total flavonoid aglycones from hickory leaves |
Non-Patent Citations (1)
| Title |
|---|
| Inhibition of chrysin on xanthine oxidase activity and its inhibition mechanism;Suyun Lin等;《International Journal of Biological Macromolecules》;20150811;第274-282页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105287709A (en) | 2016-02-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9089595B2 (en) | Extract of Rehmannia glutinasa Libosch. for reducing blood glucose and lipid levels and treating hematologic diseases, and methods for preparing the same | |
| CN100998621B (en) | Radix sileris total chromone extractive and its preparation method | |
| CN102641326B (en) | Astragalus extract, as well as preparation and application methods thereof | |
| CN104983758B (en) | A kind of medicinal usage of Fructus Terminaliae Billericae extract | |
| CN105560262A (en) | Application of Graveobioside A in preparation of drugs or healthcare food for preventing hyperuricemia and gout | |
| CN105055510B (en) | Perilla leaf inhibiting hyperuricemia active component and its preparation method and application | |
| JP2004250449A (en) | Pharmaceutical preparation containing phenyl ehtanoide glycoside extracted from cistanche tubulosa (schenk) wight belonging to the family cistanche, preparation method therefor and its use | |
| CN105287709B (en) | A kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect | |
| CN101274025A (en) | Chinese medicinal composition with functions of reducing fever, purging the intense heat and detoxicating and preparation method thereof and quality control method | |
| WO2012100612A1 (en) | Extraction method, total saponin and use of ilex kudingcha c.j.tseng leaves | |
| CN104546995B (en) | A kind of medicinal usage of emblic extract | |
| CN105963342A (en) | An antiallergic compound flavone composition, and a preparing method and applications thereof | |
| CN101978982B (en) | Application of Japanese ginseng or extract thereof to preparation of medicament for resisting gout and repairing hyperuricemic kidney injury | |
| CN105535533A (en) | Traditional Chinese medicine composition for treatinggastritis | |
| CN108743795A (en) | A kind of prevention diabetic nephropathy towards medicament extract and its preparation method and application | |
| CN105362259B (en) | A kind of Hickory Leaves extract pinostrobin with anti-trioxypurine effect and its preparation method and application | |
| CN102018741B (en) | Spanishneedles herb total flavone extract as well as preparation technology and preparation thereof | |
| CN106074636A (en) | The application of people face fruit leaf extract and preparation method | |
| CN103705772B (en) | Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof | |
| CN106038738A (en) | Docynia delavayi polyphenol extract and preparation method thereof | |
| CN107252440A (en) | A kind of preparation method and purposes of the Hickory Leaves general flavone with hypoglycemic effect | |
| CN101816702B (en) | Lung cancer preventing and treating composition extracted from selfheal and/or garden orache and application thereof in preparation of lung cancer preventing and treating medicine | |
| CN107693576A (en) | A kind of Chinese medicine composition for being used to treat coronary heart disease | |
| CN1969957B (en) | Pharmaceutical composition comprising flavescent sophora root, magnolia vine fruit and astragalus root | |
| CN110898170B (en) | Traditional Chinese medicine composition for treating metabolic syndrome and preparation thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |