CN105287709A - Pecan leaf extract total flavone with uric-acid lowering effect and preparation method and application thereof - Google Patents
Pecan leaf extract total flavone with uric-acid lowering effect and preparation method and application thereof Download PDFInfo
- Publication number
- CN105287709A CN105287709A CN201510686098.5A CN201510686098A CN105287709A CN 105287709 A CN105287709 A CN 105287709A CN 201510686098 A CN201510686098 A CN 201510686098A CN 105287709 A CN105287709 A CN 105287709A
- Authority
- CN
- China
- Prior art keywords
- total flavone
- volume
- hickory leaves
- pinostrobin
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses pecan leaf extract total flavone with uric-acid lowering effect and a preparation method and application thereof. The pecan leaf extract total flavone contains pinostrobin, pinostrobin chalcone, chrysin and cardamonin whose total mass percentage is 50% or above. The total flavone in pecan leaf is prepared on the basis of the pecan leaf, efficacy of the pecan leaf is developed, and novel medicinal resources of the pecan leaf are expanded. The pecan leaf extract total flavone can remarkably lower serum uric acid level of model rats and can be used for medicaments and healthcare products having main functions of preventing and treating hyperuricemia.
Description
Technical field
The present invention relates to medical art, particularly a kind of Hickory Leaves extractive total flavone with uric acid resisting effect and preparation method thereof and purposes.
Background technology
Uric acid is the metabolite of purine in endogenous purine and diet.In the purine metabolism of human body, ATP etc. are through a series of metabolic response, and produce hypoxanthine and xanthine, both generate uric acid under xanthine oxidase effect.When purine metabolic disturbance, serum uric acid level is too high, cause the generation of hyperuricemia (Hyperuricemia), then cause urate crystallization and be deposited in joint, gout is caused to show effect, often involve kidney and cause chronic interstitial nephritis and uric acid kidney stones, and Metabolic syndrome may be caused to seek peace relevant cardiovascular disease.In recent years, the sickness rate of hyperuricemia sharply increases, and more than 10%, united state is classified as one of 21 century 20 large pertinacious disease.
Prevent and treat hyperuricemia class drug main at present and will be divided into uricopoiesis depressant (as allopurinol) and uricosureic agent (as benzbromarone) two kinds, although these medicines have obvious uric acid resisting effect, but side effect is also larger, as erythra, heating, hepatic renal dysfunction etc., thus limit the scope of its application.Therefore, developmental research drug effect is clear and definite and the novel anti-antihyperuricemic disease drug of safety is very necessary.According to the literature, containing the composition that a large amount of xanthine oxidase inhibitor and uricosuric are drained in natural plant crude drugs source, and toxicity is little, and safety is high, has research and DEVELOPMENT PROSPECT preferably.
Chinese walnut (CaryacathayensisSarg.) is Juglandaceae hickory plant, Hickory Leaves Chang Zuowei folk tradition Chinese herbal medicine, for purposes such as heat-clearing and toxic substances removing, there are some researches show that Hickory Leaves extract contains a large amount of anti-oxidation active substance, be mainly Flavonoid substances, there is the effects such as obvious defying age, antitumor, angiogenesis inhibitor.At present, there is not yet the relevant report adopting Hickory Leaves extractive total flavone treatment hyperuricemia.Inventor is found by research, and Hickory Leaves extract pinostrobin can reduce rat model serum uric acid level, can be used for preventing and treating medicine and health food that hyperuricemia is major function.
Summary of the invention
The object of the present invention is to provide a kind of Hickory Leaves extractive total flavone with uric acid resisting effect, provide Preparation Method And The Use simultaneously.
The technical solution adopted for the present invention to solve the technical problems is:
There is a Hickory Leaves extractive total flavone for uric acid resisting effect, containing the bright gross mass percentage ratio of pinostrobin, pinostrobin chalcone, chrysin and Semen Phaseoli bandit more than 50%.
There is a preparation method for the Hickory Leaves extractive total flavone of uric acid resisting effect, comprise the following steps:
(1) get Hickory Leaves 35-65 DEG C oven drying, be crushed to 40-80 order, put into round-bottomed flask, adding concentration expressed in percentage by volume is 70-95% ethanol water, extracts 20-100 minute at 80-110 DEG C of normal pressure, repeats to extract 1-3 time; Then filter paper filtering is used, merging filtrate;
(2) filtrate is evaporated to concentration of alcohol in filtrate under 300 ± 100pa vacuum is 45-65%, centrifuging and taking supernatant, this supernatant is crossed SD200 macroporous adsorptive resins (filling SD200 aromatic series macroporous adsorbent resin), loading speed is 1BV/h-8BV/h, and loading volume is 4-8BV; At 20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, with distilled water eluting resin, elution volume is 5-10BV; At 20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, be the ethanol aqueous wash deresination of 10-30% by concentration expressed in percentage by volume, elution volume is 8-12BV; At 20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, be the ethanol aqueous wash deresination of 40-90% by concentration expressed in percentage by volume, elution volume is 8-12BV; Collect 40-90% ethanol elution, concentrated, reclaim ethanol, drying under reduced pressure, obtains Hickory Leaves extractive total flavone, and the total content that wherein pinostrobin, pinostrobin chalcone, chrysin and Semen Phaseoli bandit are bright is more than 50%.
As preferably, in step (1), 70-95% ethanol water consumption is that every g Hickory Leaves ground product adds 10mL.
As preferably, in step (2), parameter of noncentricity is: 2000-4000 rpm, centrifugal 20 minutes.The Hickory Leaves extractive total flavone with uric acid resisting effect is preparing the purposes in antihyperuricemic disease drug.
Above-mentioned Hickory Leaves extractive total flavone can with the adjuvant approved on medicament or food, adopt Modern preparations technology, make based on various dosage forms such as the capsule of Hickory Leaves extractive total flavone, tablet, powder, oral liquid, soft capsule, pill, gel, slow releasing preparation.
The invention has the beneficial effects as follows: the present invention relies on, and prepares the total flavones in Hickory Leaves, develops its effect, expands the medicine resource that Hickory Leaves is new.The experiment of inventor shows: this Hickory Leaves extractive total flavone significantly can reduce the serum uric acid level of rat model, can be used for preventing and treating medicine and health promoting product that hyperuricemia is major function.
Detailed description of the invention
Below by specific embodiment, technical scheme of the present invention is described in further detail.
In the present invention, if not refer in particular to, the raw material adopted and equipment etc. all can be buied from market or this area is conventional.Method in following embodiment, if no special instructions, is the conventional method of this area.
Embodiment
A kind of preparation method with the Hickory Leaves extractive total flavone of uric acid resisting effect, comprise the following steps: (1) gets Hickory Leaves 35-65 DEG C oven drying, be crushed to 40-80 order, put into round-bottomed flask, adding concentration expressed in percentage by volume is 70-95% ethanol water (every g Hickory Leaves ground product adds 10mL), extract 20-100 minute at 80-110 DEG C of normal pressure, repeat to extract 1-3 time; Then filter paper filtering is used, merging filtrate;
(2) filtrate concentrating under reduced pressure under 300 ± 100pa vacuum, concentration of alcohol in filtrate is made to be 45-65%, centrifugal (2000-4000 rpm, centrifugal 20 minutes) get supernatant, this supernatant is crossed SD200 macroporous adsorptive resins, loading speed is 1BV/h-8BV/h, and loading volume is 4-8BV; At 20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, with distilled water eluting resin, elution volume is 5-10BV; At 20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, be the ethanol aqueous wash deresination of 10-30% by concentration expressed in percentage by volume, elution volume is 8-12BV; At 20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, be the ethanol aqueous wash deresination of 40-90% by concentration expressed in percentage by volume, elution volume is 8-12BV; Collect 40-90% ethanol elution, concentrated, reclaim ethanol, drying under reduced pressure, obtains Hickory Leaves extractive total flavone, and the total content that wherein pinostrobin, pinostrobin chalcone, chrysin and Semen Phaseoli bandit are bright is more than 50%.
Specific embodiment 1
After Hickory Leaves drying, mechanical activation comminution, takes Hickory Leaves powder 200g, and the percent by volume adding 10 times amount (g:mL) is the alcoholic solution of 95%, extract 2 times, each 30min, merging filtrate, concentrating under reduced pressure under 300 ± 100pa vacuum, concentration of alcohol in filtrate is made to be 55%, 3000 rpms, centrifugal 20 minutes, get supernatant.Take SD200 aromatic series macroporous adsorbent resin (Zhejiang Province Zhengguan Industry Co., Ltd) 4.0g, put into chromatographic column (15mm × 350mmi.d), carry out loading with the flow velocity of 2BV/h, loading volume is 6.5BV.First use 10BV water elution, be the alcoholic solution eluting of 20% again by 10BV percent by volume, it is finally the alcoholic solution eluting of 70% by 10BV percent by volume, elution speed is 4BV/h, collect 70% ethanol elution, decompression rotary evaporated to dryness is dry, and obtain Hickory Leaves extractive total flavone, the total content that wherein pinostrobin, pinostrobin chalcone, chrysin and Semen Phaseoli bandit are bright is more than 55.3%.
Specific embodiment 2
After Hickory Leaves drying, mechanical activation comminution, takes Hickory Leaves powder 400g, and adding 10 times amount percents by volume is the alcoholic solution of 95%, extract 2 times, each 30min, merging filtrate, concentrating under reduced pressure under 300 ± 100pa vacuum, concentration of alcohol in filtrate is made to be 55%, 3000 rpms, centrifugal 20 minutes, get supernatant.Take SD200 macroporous adsorbent resin 200.0g, put into chromatographic column (15mm × 350mmi.d), carry out loading with the flow velocity of 2BV/h, loading volume is 6.5BV.First use 10BV water elution, be the alcoholic solution eluting of 20% again by 10BV percent by volume, it is finally the alcoholic solution eluting of 70% by 10BV percent by volume, elution speed is 4BV/h, collect 70% ethanol elution, decompression rotary evaporated to dryness is dry, obtains Hickory Leaves extractive total flavone, and the total content that wherein pinostrobin, pinostrobin chalcone, chrysin, Semen Phaseoli bandit are bright is more than 52.2%.
The bright total content assay method of pinostrobin, pinostrobin chalcone, chrysin, Semen Phaseoli bandit is see Shen Yong etc. 5 flavone aglycone content [J] in high effective liquid chromatography for measuring Hickory Leaves. and pharmaceutical analysis magazine, 05 phase in 2013.
Effect test
Hickory Leaves extractive total flavone finds to have reducing effect significantly to the serum uric acid level of hyperuricemia model rat prepared by lipomul through long term administration.
1 experiment material and instrument
1.1 materials and instrument
1.1 animal SD rats, male, body weight (200-500g), 60.The quality certification number: scxk (Zhejiang) 20080033.
1.2 Pharmaceutical formulations
1.2.1 by reagent
Hickory Leaves extractive total flavone, being mixed with concentration with 0.3%CMC-Na is 200mg/kg and 100.0mg/kg.
1.2.2 positive drug allopurinol tablet, specification 100mg × 200 slice, pressure pharmaceutical factory, Jiangsu side Co., Ltd, 10mg/kg and benzbromarone tablets, specification 50mg × 10 slice, the large pharmaceutical factory of German He Man, 5mg/kg.
1.3 instrument
Electronic balance CP114 (Shanghai Ao Haosi Instrument Ltd.); Electric jacket (Nantong Li Hao experimental apparatus company limited); GG17 Rotary Evaporators (Shensheng Science & Tech. Co., Ltd., Shanghai); The automatic dual pure water distillator (Shanghai Yarong Biochemical Instrument Plant) of SZ-93A; Electric refrigerator (Qingdao HaiEr Co., Ltd); Dionex high performance liquid chromatography (Dai An company of the U.S.); TBA-AOFR (ACCUTE) automatic clinical chemistry analyzer (wide medical Co., Ltd. of Japanese Toshiba three).
2 experimental techniques
2.1 administration
According to body weight, 60 rats are divided into normal group at random, model control group, positive controls, Hickory Leaves extractive total flavone high dose group (200mg/kg), Hickory Leaves extractive total flavone low dose group (100mg/kg), normal group gives purified water, and model group gives lipomul.Gastric infusion, model group and normal stock gavage give tap water, and administration group gives corresponding medicine, and administration capacity is 1mL/100g body weight.
2.2 observation index
Successive administration 8 weeks, gets hematometry rat blood serum uric acid (UA) level in every 2 weeks.
2.3 statistics and analysis
Measurement data data result with
represent, group difference adopts t-test.
3 experimental results
Normal group, model group and each administration group serum uric acid situation of change during 3.1 administrations
Compared with normal group, model group rats serum uric acid obviously raises (P<0.01); Compared with model group, administration 4 weeks Hickory Leaves extractive total flavone high low dose group obviously can reduce model group rats UA level (P<0.05), and uric acid resisting effect steadily continues.The results are shown in Table 1.
The change (X ± S, n=10) of table 1 successive administration rat UA level
Note: compared with normal group, * P<0.05, * * P<0.01; Compared with model control group, #P<0.05, ##P<0.01.
Above-described embodiment is one of the present invention preferably scheme, not does any pro forma restriction to the present invention, also has other variant and remodeling under the prerequisite not exceeding the technical scheme described in claim.
Claims (5)
1. there is a Hickory Leaves extractive total flavone for uric acid resisting effect, it is characterized in that: containing the bright gross mass percentage ratio of pinostrobin, pinostrobin chalcone, chrysin and Semen Phaseoli bandit more than 50%.
2. there is a preparation method for the Hickory Leaves extractive total flavone of uric acid resisting effect as claimed in claim 1, it is characterized in that, comprise the following steps:
(1) get Hickory Leaves 35-65 DEG C oven drying, be crushed to 40-80 order, put into round-bottomed flask, adding concentration expressed in percentage by volume is 70-95% ethanol water, extracts 20-100 minute at 80-110 DEG C of normal pressure, repeats to extract 1-3 time; Then filter paper filtering is used, merging filtrate;
(2) filtrate is evaporated to concentration of alcohol in filtrate under 300 ± 100pa vacuum is 45-65%, and centrifuging and taking supernatant, crosses SD200 macroporous adsorptive resins by this supernatant, and loading speed is 1BV/h-8BV/h, and loading volume is 4-8BV; At 20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, with distilled water eluting resin, elution volume is 5-10BV; At 20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, be the ethanol aqueous wash deresination of 10-30% by concentration expressed in percentage by volume, elution volume is 8-12BV; At 20-35 DEG C, under normal pressure, with 1BV/h-8BV/h flow velocity, be the ethanol aqueous wash deresination of 40-90% by concentration expressed in percentage by volume, elution volume is 8-12BV; Collect 40-90% ethanol elution, concentrated, reclaim ethanol, drying under reduced pressure, obtains Hickory Leaves extractive total flavone, and the total content that wherein pinostrobin, pinostrobin chalcone, chrysin and Semen Phaseoli bandit are bright is more than 50%.
3. preparation method according to claim 2, is characterized in that: in step (1), 70-95% ethanol water consumption is that every g Hickory Leaves ground product adds 10mL.
4. preparation method according to claim 2, is characterized in that: in step (2), parameter of noncentricity is: 2000-4000 rpm, centrifugal 20 minutes.
5. the Hickory Leaves extractive total flavone as claimed in claim 1 with uric acid resisting effect is preparing the purposes in antihyperuricemic disease drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510686098.5A CN105287709B (en) | 2015-10-20 | 2015-10-20 | A kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510686098.5A CN105287709B (en) | 2015-10-20 | 2015-10-20 | A kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105287709A true CN105287709A (en) | 2016-02-03 |
| CN105287709B CN105287709B (en) | 2019-09-13 |
Family
ID=55185996
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510686098.5A Active CN105287709B (en) | 2015-10-20 | 2015-10-20 | A kind of Hickory Leaves extractive total flavone as well as preparation method and application thereof with anti-trioxypurine effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105287709B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107252440A (en) * | 2017-06-05 | 2017-10-17 | 浙江中医药大学 | A kind of preparation method and purposes of the Hickory Leaves general flavone with hypoglycemic effect |
| CN107519234A (en) * | 2017-08-28 | 2017-12-29 | 付林春 | A kind of preparation technology of Hickory Leaves extractive of general flavone |
| CN111494569A (en) * | 2020-06-19 | 2020-08-07 | 陈正 | Preparation method of walnut leaf powder instant beverage for relieving gout |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142685A (en) * | 2013-02-07 | 2013-06-12 | 浙江中医药大学 | Method for extraction of total flavonoid aglycones from hickory leaves |
-
2015
- 2015-10-20 CN CN201510686098.5A patent/CN105287709B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142685A (en) * | 2013-02-07 | 2013-06-12 | 浙江中医药大学 | Method for extraction of total flavonoid aglycones from hickory leaves |
Non-Patent Citations (1)
| Title |
|---|
| SUYUN LIN等: "Inhibition of chrysin on xanthine oxidase activity and its inhibition mechanism", 《INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107252440A (en) * | 2017-06-05 | 2017-10-17 | 浙江中医药大学 | A kind of preparation method and purposes of the Hickory Leaves general flavone with hypoglycemic effect |
| CN107519234A (en) * | 2017-08-28 | 2017-12-29 | 付林春 | A kind of preparation technology of Hickory Leaves extractive of general flavone |
| CN111494569A (en) * | 2020-06-19 | 2020-08-07 | 陈正 | Preparation method of walnut leaf powder instant beverage for relieving gout |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105287709B (en) | 2019-09-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5352655B2 (en) | Pharmaceutical preparation comprising a phenylethanoid glycoside extracted from a herbaceous plant, the all parasitic plant of the family Amaranthaceae [CISTANCHETUBULOSA (SCHENK.) WIIGHT], method for producing the same, and use thereof | |
| CN100998621B (en) | Radix sileris total chromone extractive and its preparation method | |
| US10967030B2 (en) | Traditional Chinese medicine composition for treating psoriasis and method for preparing the same | |
| CN105560262A (en) | Application of Graveobioside A in preparation of drugs or healthcare food for preventing hyperuricemia and gout | |
| CN104435226B (en) | Fevervine extract and application thereof | |
| CN104983758B (en) | A kind of medicinal usage of Fructus Terminaliae Billericae extract | |
| CN107854507B (en) | Method for extracting flavonoid component from folium artemisiae argyi | |
| CN105287709A (en) | Pecan leaf extract total flavone with uric-acid lowering effect and preparation method and application thereof | |
| CN103169788A (en) | Chinese date leaf extractive and application thereof in preparation of medicament or health food for preventing and treating liver injury | |
| CN101502579A (en) | Chinese medicinal composition for treating digestive tumor and preparation method thereof | |
| CN104546995B (en) | A kind of medicinal usage of emblic extract | |
| CN101347491A (en) | Radical lobelia total flavones effective component and preparation thereof | |
| CN103830374B (en) | The application in hyperuricemia clearly of three leaf glycolipids | |
| CN103816147B (en) | The medical usage of gamlogic acid, neogambogic acid and compositions thereof | |
| CN105362259A (en) | Chinese walnut leaf extract pinostrobin having uric acid reducing effect and preparation method thereof and application | |
| CN103705772B (en) | Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof | |
| CN102302545A (en) | Dai medicine extract with blood-sugar reducing activity, preparation method, composition and application | |
| CN106361811A (en) | Tongmai pharmaceutical composition and preparation method thereof | |
| CN103550200B (en) | Application of pharmaceutical composition in preparation of medicament for inhibiting blood sugar rise and medicament for reducing blood fat | |
| CN106074636A (en) | The application of people face fruit leaf extract and preparation method | |
| CN107252440A (en) | A kind of preparation method and purposes of the Hickory Leaves general flavone with hypoglycemic effect | |
| CN106038738A (en) | Docynia delavayi polyphenol extract and preparation method thereof | |
| CN105380985B (en) | Pharmaceutical composition for treating cerebral arterial thrombosis | |
| CN102232982A (en) | Extract product of herba cephalanoploris effective position, preparation method thereof and purpose thereof | |
| CN102631386A (en) | Bupleurum antipyretic and analgesic preparation and technology for preparing same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |