CN107252440A - A kind of preparation method and purposes of the Hickory Leaves general flavone with hypoglycemic effect - Google Patents
A kind of preparation method and purposes of the Hickory Leaves general flavone with hypoglycemic effect Download PDFInfo
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- CN107252440A CN107252440A CN201710415348.0A CN201710415348A CN107252440A CN 107252440 A CN107252440 A CN 107252440A CN 201710415348 A CN201710415348 A CN 201710415348A CN 107252440 A CN107252440 A CN 107252440A
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- general flavone
- hickory leaves
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- hickory
- hypoglycemic effect
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- 241000723418 Carya Species 0.000 title claims abstract description 47
- 229930003944 flavone Natural products 0.000 title claims abstract description 35
- 235000011949 flavones Nutrition 0.000 title claims abstract description 35
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 35
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 34
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 230000002218 hypoglycaemic effect Effects 0.000 title claims abstract description 15
- 239000003814 drug Substances 0.000 claims abstract description 10
- 239000000284 extract Substances 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 52
- 238000010828 elution Methods 0.000 claims description 13
- 239000000706 filtrate Substances 0.000 claims description 12
- 210000004369 blood Anatomy 0.000 claims description 10
- 239000008280 blood Substances 0.000 claims description 10
- 238000011068 loading method Methods 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 229920005989 resin Polymers 0.000 claims description 5
- 238000010936 aqueous wash Methods 0.000 claims description 4
- 230000006837 decompression Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 3
- 241000899834 Obovaria olivaria Species 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- 239000003405 delayed action preparation Substances 0.000 claims description 2
- 238000004821 distillation Methods 0.000 claims description 2
- 239000003480 eluent Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 235000013305 food Nutrition 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 239000000499 gel Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 238000000643 oven drying Methods 0.000 claims description 2
- 239000006187 pill Substances 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 239000007901 soft capsule Substances 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- 240000005809 Prunus persica Species 0.000 claims 1
- 235000006040 Prunus persica var persica Nutrition 0.000 claims 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims 1
- 229930003935 flavonoid Natural products 0.000 claims 1
- 150000002215 flavonoids Chemical class 0.000 claims 1
- 235000017173 flavonoids Nutrition 0.000 claims 1
- 206010012601 diabetes mellitus Diseases 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 2
- 235000013402 health food Nutrition 0.000 abstract 1
- HIMXGTXNXJYFGB-UHFFFAOYSA-N alloxan Chemical compound O=C1NC(=O)C(=O)C(=O)N1 HIMXGTXNXJYFGB-UHFFFAOYSA-N 0.000 description 10
- RTIXKCRFFJGDFG-UHFFFAOYSA-N chrysin Chemical compound C=1C(O)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=CC=C1 RTIXKCRFFJGDFG-UHFFFAOYSA-N 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 201000001421 hyperglycemia Diseases 0.000 description 7
- CUGDOWNTXKLQMD-BQYQJAHWSA-N Pinostrobin chalcone Chemical compound OC1=CC(OC)=CC(O)=C1C(=O)\C=C\C1=CC=CC=C1 CUGDOWNTXKLQMD-BQYQJAHWSA-N 0.000 description 6
- ORJDDOBAOGKRJV-CQSZACIVSA-N (2S)-Pinocembrin Natural products C1([C@H]2CC(=O)C3=C(O)C=C(C=C3O2)OC)=CC=CC=C1 ORJDDOBAOGKRJV-CQSZACIVSA-N 0.000 description 5
- ORJDDOBAOGKRJV-UHFFFAOYSA-N Dihydrotectochrysin Natural products O1C2=CC(OC)=CC(O)=C2C(=O)CC1C1=CC=CC=C1 ORJDDOBAOGKRJV-UHFFFAOYSA-N 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- ORJDDOBAOGKRJV-AWEZNQCLSA-N pinostrobin Chemical compound C1([C@@H]2CC(=O)C3=C(O)C=C(C=C3O2)OC)=CC=CC=C1 ORJDDOBAOGKRJV-AWEZNQCLSA-N 0.000 description 5
- NYCXYKOXLNBYID-UHFFFAOYSA-N 5,7-Dihydroxychromone Natural products O1C=CC(=O)C=2C1=CC(O)=CC=2O NYCXYKOXLNBYID-UHFFFAOYSA-N 0.000 description 4
- 240000001417 Vigna umbellata Species 0.000 description 4
- 235000011453 Vigna umbellata Nutrition 0.000 description 4
- 235000015838 chrysin Nutrition 0.000 description 4
- 229940043370 chrysin Drugs 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- CUGDOWNTXKLQMD-UHFFFAOYSA-N Pinostrobinchalkon Natural products OC1=CC(OC)=CC(O)=C1C(=O)C=CC1=CC=CC=C1 CUGDOWNTXKLQMD-UHFFFAOYSA-N 0.000 description 3
- 239000002250 absorbent Substances 0.000 description 3
- 230000002745 absorbent Effects 0.000 description 3
- -1 flavone compound Chemical class 0.000 description 3
- IXZISFNWUWKBOM-ARQDHWQXSA-N fructosamine Chemical compound NC[C@@]1(O)OC[C@@H](O)[C@@H](O)[C@@H]1O IXZISFNWUWKBOM-ARQDHWQXSA-N 0.000 description 3
- 238000000034 method Methods 0.000 description 3
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- 241000305527 Juglans cathayensis Species 0.000 description 2
- 235000014075 Juglans mandschurica Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000013118 diabetic mouse model Methods 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 1
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 1
- 201000004569 Blindness Diseases 0.000 description 1
- DQFBYFPFKXHELB-UHFFFAOYSA-N Chalcone Natural products C=1C=CC=CC=1C(=O)C=CC1=CC=CC=C1 DQFBYFPFKXHELB-UHFFFAOYSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 241000758791 Juglandaceae Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010029164 Nephrotic syndrome Diseases 0.000 description 1
- 208000022329 Protein metabolism disease Diseases 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000130764 Tinea Species 0.000 description 1
- 208000002474 Tinea Diseases 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003527 anti-angiogenesis Effects 0.000 description 1
- 230000001139 anti-pruritic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003472 antidiabetic agent Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000005513 chalcones Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 125000004073 flavone group Chemical group 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 208000037890 multiple organ injury Diseases 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 208000009928 nephrosis Diseases 0.000 description 1
- 231100001027 nephrosis Toxicity 0.000 description 1
- 238000005220 pharmaceutical analysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000012109 statistical procedure Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- DQFBYFPFKXHELB-VAWYXSNFSA-N trans-chalcone Chemical compound C=1C=CC=CC=1C(=O)\C=C\C1=CC=CC=C1 DQFBYFPFKXHELB-VAWYXSNFSA-N 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/52—Juglandaceae (Walnut family)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medical Informatics (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of preparation method and purposes of the Hickory Leaves chromocor extract with hypoglycemic effect, the Hickory Leaves general flavone of the present invention shows good hypoglycemic effect in each dosage, there is remarkable effect to diabetes, available for medicine, health food for preparing prevention or treatment diabetes etc..
Description
Technical field
The present invention relates to technical field of traditional Chinese medicines, the system of more particularly to a kind of Hickory Leaves general flavone with hypoglycemic effect
Preparation Method and purposes.
Background technology
Diabetes are one group of metabolic diseases being characterized with hyperglycaemia, are clinically mainly shown as chronic hyperglycemia and companion
There are carbohydrate, fat, protein metabolism disorder etc..Its course of disease is longer, if cannot timely and effectively treat, it is likely that
Cause whole body multiple organ injury, the complication such as infection, blindness, nephrosis occur, seriously endanger health.Sent out because it is extensive
Patient group and the incidence of disease being gradually increasing in recent years, diabetes have become the harm mankind after angiocardiopathy and cancer and are good for
The third-largest disease of health.For this present situation, a large amount of hypoglycemic drugs come out therewith, but clinically common treating diabetes at present
Medicine is generally chemical synthesis class medicine, although determined curative effect, also there is more adverse reaction simultaneously.Natural drug, is particularly me
State's Chinese traditional herbs, under this background with advantageous superiority condition, therefore research and develop the natural of high-efficiency low-toxicity
Medicine, it has also become the focus of prevention and treatment diabetes and complication.
Chinese walnut is the leaf of Juglandaceae hickory nut, with the antipruritic effect of clearing heat and detoxicating, desinsection, it is among the people often by
Treatment toe it is wet itch, skin tinea card etc..In addition literature research shows in Hickory Leaves extract containing antioxygens such as substantial amounts of flavones
Change active material, with the effect such as antitumor, anti-aging, anti-mutagenesis, anti-angiogenesis.And Chinese walnut be in itself a class for many years
Raw deciduous tree, its leaf is often dropped in planting process, therefore exploitation Hickory Leaves are with very big economic value and market
Potentiality.
The content of the invention
It is an object of the invention to provide a kind of Hickory Leaves general flavone extraction using flavone compound as main component
Thing；
The present invention provides the preparation method of above-mentioned Hickory Leaves extractive of general flavone；
The present invention provides above-mentioned Hickory Leaves extractive of general flavone and is preventing or treating the medicine of blood glucose rise disease or health care
Purposes in product preparation.
To achieve the above object, the technical scheme that the present invention takes is as follows：
A kind of preparation method of the Hickory Leaves general flavone with hypoglycemic effect, the preparation method comprises the following steps：
(1) 40 DEG C of oven dryings of Hickory Leaves are taken, 40 mesh sieves are crossed, 80-100 DEG C of normal pressure is extracted in 95% ethanol and extracts 30-90
Minute, repeat 1-3 times, filtering, merging filtrate；
(2) filtrate decompression is concentrated, and this supernatant is crossed SD200 large pore resin absorption columns by centrifuging and taking supernatant, and loading speed is
1BV/h-8BV/h, loading volume is 4-8BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocitys, with distillation water elution tree
Fat, elution volume is 5-10BV；It is 10- with concentration expressed in percentage by volume with 1BV/h-8BV/h flow velocitys under 20-35 DEG C, normal pressure
30% ethanol aqueous wash deresination, elution volume is 8-12BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocitys,
The ethanol aqueous wash deresination for being 40-90% with concentration expressed in percentage by volume, elution volume is 8-12BV；Collect 40-90% ethanol
Eluent, concentration reclaims ethanol, is dried under reduced pressure, obtains Hickory Leaves extract.
Preferably, 70-95% ethanol waters consumption is to add 10mL per 1g Hickory Leaves crushed materials in step (1).
Preferably, parameter of noncentricity is in step (2)：2000-4000 rpms, centrifuge 20 minutes.
Preferably, concentration makes concentration of alcohol in filtrate be 45-65% in step (2).
A kind of purposes of the Hickory Leaves general flavone with hypoglycemic effect, the Hickory Leaves general flavone of above-mentioned preparation is carried
Take thing, using Modern preparations technology, can be made with the auxiliary material approved on medicament or food based on Hickory Leaves general flavone
The various formulations such as capsule, tablet, powder, oral liquid, soft capsule, pill, gel, sustained release preparation；The preparation is used for
Clinical treatment blood glucose rise and the health-caring product capable of reducing blood sugar that the general flavone containing Hickory Leaves is made.
Hickory Leaves general flavone prepared by the present invention is evident in efficacy in terms of blood glucose is reduced, and causes diabetes small with alloxan
Mouse model experimental result shows that Hickory Leaves general flavone has significant hypoglycemic effect.
The advantage of the invention is that the present invention uses method simple economy, production cost is low, and gained general flavone content is higher
And steady quality.
Embodiment
Below by specific embodiment, technical scheme is described in further detail, but not office of the invention
It is limited to these embodiments.
In the present invention, if not refering in particular to, raw material and equipment used etc. is commercially available or commonly used in the art.
Method in following embodiments, is the conventional method of this area unless otherwise instructed.
Embodiment 1
After Hickory Leaves are dried, mechanical crushing weighs Hickory Leaves powder 200g, plus 10 times of amount (g：ML percent by volume) is
95% ethanol solution, is extracted 2 times, each 30min, merging filtrate is concentrated under reduced pressure under 300 ± 100pa vacuums, makes filtrate
Middle concentration of alcohol is 65%, 3000 rpms, centrifuges 20 minutes, takes supernatant.Weigh SD200 aromatic series macroporous absorbent resins
(Zhejiang Province Zhengguan Industry Co., Ltd) 4.0g, is put into chromatographic column (15mm × 350mm i.d), is entered with 2BV/h flow velocity
Row loading, loading volume is 6.5BV.10BV water elutions are first used, then are eluted with 10BV percents by volume for 20% ethanol solution,
Finally eluted with 10BV percents by volume for 70% ethanol solution, elution speed is 4BV/h, collect 70% ethanol eluate,
Depressurize rotary evaporated to dryness it is dry, obtain Hickory Leaves extractive total flavone, wherein pinostrobin, pinostrobin chalcone, Chrysin and
The bright total content of red bean bandit is more than 55.3%.
Embodiment 2
After Hickory Leaves are dried, mechanical crushing weighs Hickory Leaves powder 400g, plus the second that 10 times of amount percents by volume are 95%
Alcoholic solution, is extracted 2 times, each 30min, merging filtrate is concentrated under reduced pressure under 300 ± 100pa vacuums, makes ethanol in filtrate dense
Spend for 55%, 4000 rpms, centrifuge 20 minutes, take supernatant.SD200 macroporous absorbent resin 200.0g are weighed, chromatography is put into
In post (15mm × 350mm i.d), successively with 10BV water, 20% ethanol, 40% ethanol, 70% ethanol elution, and collect
70% ethanol eluate, decompression rotary evaporated to dryness is dry, produces Hickory Leaves extractive of general flavone, wherein pinostrobin, pinostrobin
The bright total content of chalcone, Chrysin, red bean bandit is more than 52.2%.
Embodiment 3
After Hickory Leaves are dried, mechanical crushing weighs Hickory Leaves powder 200g, plus 10 times of amount (g：ML percent by volume) is
95% ethanol solution, is extracted 3 times, each 90min, merging filtrate is concentrated under reduced pressure under 300 ± 100pa vacuums, makes filtrate
Middle concentration of alcohol is 65%, 2000 rpms, centrifuges 20 minutes, takes supernatant.Weigh SD200 aromatic series macroporous absorbent resins
(Zhejiang Province Zhengguan Industry Co., Ltd) 4.0g, is put into chromatographic column (15mm × 350mm i.d), is entered with 8BV/h flow velocity
Row loading, loading volume is 8BV.First use 5BV water elutions, then using ethanol of the 1BV/h flow velocitys with 12BV percents by volume as 30%
Solution is eluted, and is finally eluted using 5BV/h flow velocitys with 10BV percents by volume as 90% ethanol solution, is collected 90% ethanol elution
Liquid, decompression rotary evaporated to dryness is dry, obtains Hickory Leaves extractive total flavone, wherein pinostrobin, pinostrobin chalcone, Chrysin
And the bright total content of red bean bandit is more than 54.3%.
The bright total content assay method of pinostrobin, pinostrobin chalcone, Chrysin, red bean bandit is referring to the efficient liquid of the such as Shen Yong
Phase chromatography determines 5 flavone aglycone content [J] Pharmaceutical Analysis magazines, 05 phase in 2013 in Hickory Leaves.
Diabetic mouse model is caused to carry out hypoglycemic reality alloxan the gained Hickory Leaves general flavone of above-described embodiment 1
Test research.
Experimental method is as follows：
1 experiment material and instrument
1.1 experimental animal
18~22g health cleaning grade male ICR mouses
1.2 reagents and instrument
1.2.1 reagent
Hickory Leaves general flavone, CMC-Na, alloxan, melbine, Glucose estimation kit, Values of The Serum Fructosamine examination
Agent box etc..
1.2.1 instrument
Semi-automatic biochemical analyzer；SENCOR-201 rotary evaporators；Electronic balance；Centrifuge etc..
2 experimental methods
By male ICR mouse 50,6 groups, wherein blank control group 10, each 8 of remaining each group, by 40 models are randomly divided into
Tail vein injection alloxan solution 80mg/kg, which makes, after mouse fasting 12h touches (Normal group injection normal saline), 5d
After survey blood glucose, work as blood glucose>Modeling success during 11.1mmol/L.Then model mice is randomly divided into 5 groups, respectively hyperglycaemia mould
Type group, Hickory Leaves general flavone 50mg/kg low dose groups, 100mg/kg middle dose groups, 200mg/kg high dose groups, 50mg/kg
Melbine positive drug group.Normal group and model group give distilled water gavage during experiment, and remaining each group presses dosage
Gavage, 1 time/d, continuous 14d.After 14d, each group mouse blood sugar, body weight and Serum Fructosamine are determined, and record.Experimental result is equal
Statistical procedures are carried out using the statistical softwares of SPSS 18.0.Experimental data is represented with average ± standard deviation, using single factor test
Variance analysis, thinks there is significant difference during P ＜ 0.05.
Experimental result
Influence of the Hickory Leaves general flavone of table 1 to hyperglycemia model mouse
Note：Compared with normal group,**P<0.01；Compared with model group,#P<0.05,##P<0.01。
Influence of the Hickory Leaves general flavone different dosing dosage of table 2 to hyperglycaemia mouse weight
Note：Compared with normal group,**P<0.01；Compared with model group,#P<0.05。
Influence of the Hickory Leaves general flavone of table 3 to hyperglycemia model mice serum fructose amine content
Note：Compared with normal group**P<0.01；Compared with model group#P<0.05；##P<0.01。
Analysis of experimental results：The study show that the low middle high dose group hyperglycemia model of positive drug group, Hickory Leaves general flavone
Group mouse is compared, and blood sugar level is decreased significantly, and Serum Fructosamine level is significantly reduced and there is dose dependent, i.e.,
Test result indicates that Hickory Leaves general flavone causes diabetic mouse model to have notable hypoglycemic effect alloxan.
Embodiment described above is a kind of preferably scheme of the present invention, not makees any formal to the present invention
Limitation, also has other variants and remodeling on the premise of without departing from the technical scheme described in claim.
Claims (5)
1. a kind of preparation method of the Hickory Leaves general flavone with hypoglycemic effect, it is characterised in that the preparation method bag
Include following steps：
(1) 40 DEG C of oven dryings of Hickory Leaves are taken, 40 mesh sieves are crossed, 80-100 DEG C of normal pressure is extracted in 95% ethanol and extracts 30-90
Minute, repeat 1-3 times, filtering, merging filtrate；
(2) filtrate decompression is concentrated, and this supernatant is crossed SD200 large pore resin absorption columns by centrifuging and taking supernatant, and loading speed is
1BV/h-8BV/h, loading volume is 4-8BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocitys, with distillation water elution tree
Fat, elution volume is 5-10BV；It is 10- with concentration expressed in percentage by volume with 1BV/h-8BV/h flow velocitys under 20-35 DEG C, normal pressure
30% ethanol aqueous wash deresination, elution volume is 8-12BV；Under 20-35 DEG C, normal pressure, with 1BV/h-8BV/h flow velocitys,
The ethanol aqueous wash deresination for being 40-90% with concentration expressed in percentage by volume, elution volume is 8-12BV；Collect 40-90% ethanol
Eluent, concentration reclaims ethanol, is dried under reduced pressure, obtains Hickory Leaves extract.
2. a kind of preparation method of Hickory Leaves general flavone with hypoglycemic effect according to claim 1, its feature
It is, 95% ethanol water consumption is to add 10mL per 1g Hickory Leaves crushed materials in step (1).
3. a kind of preparation method of Hickory Leaves general flavone with hypoglycemic effect according to claim 1, its feature
It is, parameter of noncentricity is in step (2)：2000-4000 rpms, centrifuge 20 minutes.
4. a kind of preparation method of Hickory Leaves general flavone with hypoglycemic effect according to claim 1, its feature
It is, concentration makes concentration of alcohol in filtrate be 45-65% in step (2).
5. a kind of purposes of the Hickory Leaves general flavone with hypoglycemic effect, it is characterised in that mountain core prepared by claim 1
Peach leaf extractive of general flavone, using Modern preparations technology, can be made with hickory nut with the auxiliary material approved on medicament or food
The various formulations such as capsule, tablet, powder, oral liquid, soft capsule, pill, gel, sustained release preparation based on leaf flavonoids；
The preparation is used for clinical treatment blood glucose rise and the health-caring product capable of reducing blood sugar of the general flavone containing Hickory Leaves is made.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109232757A (en) * | 2018-08-31 | 2019-01-18 | 吉首大学 | Walnut Leaves polyoses extract and application |
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| CN1322132A (en) * | 1998-09-15 | 2001-11-14 | 韩国科学技术研究院 | Bioflavonoid as blood gloucose level lowering agent |
| CN1582919A (en) * | 2004-06-08 | 2005-02-23 | 华东师范大学 | Preparation of medicines for reducing blood sugar and triglyceride from hippophae rhamnoides flavone |
| CN105287709A (en) * | 2015-10-20 | 2016-02-03 | 浙江中医药大学 | Pecan leaf extract total flavone with uric-acid lowering effect and preparation method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1322132A (en) * | 1998-09-15 | 2001-11-14 | 韩国科学技术研究院 | Bioflavonoid as blood gloucose level lowering agent |
| CN1582919A (en) * | 2004-06-08 | 2005-02-23 | 华东师范大学 | Preparation of medicines for reducing blood sugar and triglyceride from hippophae rhamnoides flavone |
| CN105287709A (en) * | 2015-10-20 | 2016-02-03 | 浙江中医药大学 | Pecan leaf extract total flavone with uric-acid lowering effect and preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109232757A (en) * | 2018-08-31 | 2019-01-18 | 吉首大学 | Walnut Leaves polyoses extract and application |
| CN109232757B (en) * | 2018-08-31 | 2020-12-01 | 吉首大学 | Walnut leaf polysaccharide extract and application thereof |
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