CN105287342A - External skin care composition with functions of moisture retention and skin tightening and its emulsion and preparation method - Google Patents

Abstract

The invention discloses an external skin care composition with functions of moisture retention and skin tightening. The external skin care composition comprises a phase A, a phase B and a phase C. The phases A, B and C are prepared from raw materials according to weight percentages. The phase C comprises traditional Chinese medicine extract. The traditional Chinese medicine extract is prepared from 2-10 parts by weight of dendrobium, 1-6 parts by weight of oat, 1-3 parts by weight of clove, 2-4 parts by weight of yerbadetajo herb, 2-5 parts by weight of stemona and 1-5 parts by weight of honey. An experiment proves that the external skin care composition is safe for skin, has no irritation and has effects of nourishing and tightening skin, keeping skin moisture and repairing skin, balancing fat, preventing UV and electromagnetic radiation, making skin plump, moist and glossy, and maintaining beauty and caring skin, and safely and effectively solves pregnancy woman skin problems. The external traditional Chinese medicine composition has the advantages of easily available raw materials, simple preparation processes, safety, environmental friendliness, and good application prospect and market prospect.

Description

There is moisturizing to compact the external skin-care composition, emulsion and preparation method thereof of effect

Technical field

The present invention relates to a kind of external skin-care composition, specifically a kind of have nourish compact, potent reparation, Sage Extract, the external skin-care composition of anti-ultraviolet effect and the external-use skin care preparation that prepared by said composition.

Background technology

Commercially available skin-protection product effect of a great variety is different, but a lot of skin care item easily cause the safety issues such as skin allergy due to its content problem, especially for anemia of pregnant woman and child, anemia of pregnant woman is in the process breeding life, metabolism accelerates, add the specific response in pregnancy period, skin state is worsened, easily occurs the problems such as drying is coarse, cutis laxa, mottle, pregnant occasion stricture of vagina.Along with people are to the enhancing of skin care item safety consciousness, anemia of pregnant woman is with skin care item safety and both effectivenessly receive much concern.And Chinese medicine extract have stable effect lasting, evident in efficacy, to skin action temperature and and the advantage such as safety is high, thus, consumer favors the green skin care item of the natural type refined by Chinese herbal medicine more.

In order to tackle pregnancy period scaly dry skin and the ultimate attainment safe two fold problem of product, develop a have nourish compact, potent reparation, the natural moisture preserving of Sage Extract effect, the Chinese medicine cosmetic of UV resistance effect have the meaning of reality and wide market prospect.

Summary of the invention

Primary and foremost purpose of the present invention is to propose a kind ofly to be had moisturizing and to compact the skin care compositions of effect.

Another object of the present invention is the preparation method proposing a kind of above-mentioned skin care compositions and the emulsion obtained.

Invention thinking of the present invention is:

The present invention utilizes advanced biotechnology, Herba Dendrobii, Herba bromi japonici, Flos Caryophylli, Herba Ecliptae, the Radix Stemonae and Mel are effectively combined, forms compound recipe, and it can be used as active component, be prepared into skin care formulation, it has moisturizing and nourishing, compacts and repair and uvioresistant effect.Above-mentioned each Chinese medicinal components brief introduction is as follows:

Herba Dendrobii: another name woods orchid, taboo life, Du Lan, stone Weed, outstanding bamboo, bamboo in thousand, its Latin is called HerbaDendrobiiNobilis.Both can be used for treating consumption of body fluid caused by febrile disease; Xerostomia excessive thirst; Deficiency of stomach-Yin; Stomachache is retched; Dryness of the lung dry cough; Deficiency-heat is not moved back; It is dark that the moon hinders order; Waist knee joint weakness waits disease, and can drink by daily bubble again, promote the production of body fluid stomach reinforcing; Nourishing YIN and clearing away heat; Lung moistening kidney tonifying; The strong waist of improving eyesight.

Herba bromi japonici: another name Herba bromi japonici, nature and flavor, sweet; Flat.Herba bromi japonici reduces blood pressure, reduce cholesterol, the medical value of control colorectal cancer, control heart disease and health-care effect.

Flos Caryophylli: another name clove tree, dismemberent perfume, male Flos Caryophylli, Flos Caryophylli.Acrid in the mouth; Warm in nature, warming middle-JIAO to send down the adverse QI; Warming the kidney to activate YANG.For the alabastrum of dicotyledon medicine plant clove of myrtaceae.

Herba Ecliptae: another name Herba Ecliptae, nature and flavor: sweet; Acid; Return liver, kidney channel; Cure mainly: nourishing the liver and kidney, cooling blood for hemostasis, various haematemesis can be controlled, the diseases such as enterorrhagia.

The Radix Stemonae: sweet-bitter flavor; Tepor; Return lung meridian; For the root of Stemonaceae plant Radix Stemonae, Radix stemonae japonicae and radix stemonae tuberosae.There is antibacterial, insecticidal action.

Mel: sweet in the mouth; Property is put down.There is effect of harmonizing the spleen and stomach, relieving spasm to stop pain, nourishing the lung to arrest cough, loosening bowel to relieve constipation and removing toxic substances.

For realizing object of the present invention, the present invention adopts following concrete technical scheme:

Have moisturizing to compact the external skin-care composition of effect, wherein, described skin care compositions is by A phase, B phase and C phase composition, and described A phase, B phase and C phase are made up of the raw material of following weight percentage ratio respectively:

Described Chinese medicine extract is made up of the crude drug of following weight parts proportioning:

Herba Dendrobii 2 ~ 10, Herba bromi japonici 1 ~ 6, Flos Caryophylli 1 ~ 3, Herba Ecliptae 2 ~ 4, the Radix Stemonae 2 ~ 5, Mel 1 ~ 5.

The preparation method of above-mentioned Chinese medicine extract is as follows:

(1) in above-mentioned raw materials medicine, Herba Dendrobii, Herba bromi japonici, Flos Caryophylli, Herba Ecliptae, the Radix Stemonae take by described weight after pulverizing, mixing;

(2) extracting in water: the mass ratio of crude drug and water is 1:50 ~ 1:100,60 DEG C ~ 100 DEG C are extracted 1 ~ 4 hour to obtain extracting solution;

(3) extracting solution that step (2) obtains is cooled to 20 ~ 30 DEG C, 100 ~ 200 orders filter, and leach medicinal residues final vacuum sucking filtration, collect filtrate, add Mel, after mixing, to obtain final product.

Described step (1) Raw medicated powder is broken to 10 ~ 60 orders.

The mass ratio of described step (2) Raw medicine and water is 1:60 ~ 1:80.

In described step (3), vacuum filtration condition for spread two layers of filter paper in buchner funnel, and filter paper therebetween 0.3 ~ 0.6cm kieselguhr, vacuum-0.095MPa, carries out vacuum filtration.

A preparation method for above-mentioned skin care compositions, wherein, described method comprises the steps:

(1) A heat phase is to 75 ~ 85 DEG C, stirs, be incubated 20 ~ 60 minutes for subsequent use;

(2) after B phase carbomer and aqueous dispersion hydration fully, add other raw materials of B phase, be heated to 75 ~ 85 DEG C and stir, be incubated 20 ~ 60min for subsequent use;

(3) B phase homogenizing 2000 ~ 5000 revs/min, is added in B phase by A, homogenizing 3 ~ 8 minutes; Stir 30 ~ 50 revs/min of coolings, cool to 45 ~ 50 DEG C, add the sodium hydroxide in C phase, stir;

(4) add Chinese medicine extract and hyaluronate sodium described in C phase, 30 ~ 50 revs/min, stir;

(5) 30 ~ 50 revs/min are stirred cooling, are cooled to less than 45 DEG C, add Methylisothiazolinone/iodine propilolic alcohol butyl mephenesin Carbamate, stir.

The preparation that above-mentioned raw materials and method prepare is facial treatment milk.Different according to use approach, external medicine composition of the present invention obtains active extract after extracting, it also can be made into other external-use skin care preparations, as cream frost, astringent, essential oils, facial film etc., but be not limited to above-mentioned dosage form, various different external preparation can be made according to method known in cosmetics industry field.

The preparation method of Chinese medicine composition of the present invention is not limited to of the present invention, and in this area, other conventional methods also can realize described effect, and the method for the invention is the better preparation method of inventor's recommendation.

Invention formulation recommendation method is: after clean of every day, impose on human skin with smearing method, and massage is until absorb gently.

Beneficial effect of the present invention:

The present invention confirms non-stimulated to skin safe through experiment, have effective nourish compact, moisturizing reparation, Sage Extract, UV resistance effect, make turgor moist, plentiful bright and new, reach effect of beauty and skin care.The present invention also has effect of anti-electricity device radiation, effectively can resist the infringement of extraneous pessimal stimulation to skin.Raw material of the present invention obtains easily, preparation method is easy, and green safety, has good application prospect and market prospect.

Accompanying drawing explanation

Fig. 1 is the hydration rate figure of facial treatment milk prepared by embodiment 4;

Fig. 2 is that the water of facial treatment milk prepared by embodiment 4 falls apart rate figure;

Fig. 3 is the tightening effect figure of facial treatment milk prepared by embodiment 5;

Fig. 4 is the uvioresistant injury experiment result figure of facial treatment milk prepared by embodiment 4;

Fig. 5 is the control oil design sketch of facial treatment milk prepared by embodiment 4.

Detailed description of the invention

The present embodiment medical material used all can be bought from China Medicament Group Corp. and obtain, and meets Pharmacopoeia of People's Republic of China 2010 editions standards.Other raw materials all obtain by commercially available purchase, and the present invention uses the source of raw material in table 1, and the instrument title that the present invention is used and producer are in table 2.

Table 1

Table 2

Title	Model	Producer
			Thermostat water bath	HH·S1-M	Chang'an, Beijing scientific instrument factory
High-speed multifunctional pulverizer	JP-400B-8	Yongkang City nine grades of rank in the feudal regimes Trade Co., Ltd.
			Intelligent temperature control heating stirrer	SZCL	Yu Hua Instrument Ltd. of Gongyi City
Circulating water type vacuum pump	SHB-Ⅲ	Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd.

The preparation of embodiment 1 Chinese medicine extract of the present invention

(1) take by described proportioning after being crushed to 10 orders according to following raw material medicaments (except Mel), mixing;

Herba Dendrobii 6g, Herba bromi japonici 3g, Flos Caryophylli 2g, Herba Ecliptae 4g, Radix Stemonae 5g, Mel 3g;

(2) crude drug extracting in water, the mass ratio of crude drug and water is 1:70, and 80 DEG C are extracted 2 hours;

(3) extracting solution that step (2) obtains is cooled to 20 DEG C, 100 orders filter, and carry out vacuum filtration after leaching medicinal residues, condition is: in buchner funnel, spread two layers of filter paper, filter paper therebetween 0.5cm kieselguhr, carries out vacuum filtration by coarse filtration gained filtrate, collects filtrate; Add Mel, after mixing, to obtain final product.

The preparation of embodiment 2 Chinese medicine extract of the present invention

(1) take by described weight after being crushed to 50 orders according to following raw material medicaments (except Mel), mixing;

Herba Dendrobii 2g, Herba bromi japonici 6g, Flos Caryophylli 1g, Herba Ecliptae 3g, Radix Stemonae 2g, Mel 1g;

(2) crude drug extracting in water, the mass ratio of crude drug and water is 1:50, and 100 DEG C are extracted 4 hours;

(3) extracting solution that step (2) obtains is cooled to 30 DEG C, 200 orders filter, vacuum filtration is carried out after leaching medicinal residues, condition is: in buchner funnel, spread two layers of filter paper, filter paper therebetween 0.3cm kieselguhr, and coarse filtration gained filtrate is carried out vacuum filtration, collect filtrate, add Mel, after mixing, to obtain final product.

The preparation of embodiment 3 Chinese medicine composition of the present invention

(1) take by described weight after being crushed to 60 orders according to following raw material medicaments (except Mel), mixing;

Herba Dendrobii 10g, Herba bromi japonici 1g, Flos Caryophylli 3g, Herba Ecliptae 2g, Radix Stemonae 2.5g, Mel 5g;

(2) crude drug extracting in water, the mass ratio of crude drug and water is 1:100, and 60 DEG C are extracted 1 hour;

(3) extracting solution that step (2) obtains is cooled to 25 DEG C, 100 orders filter, and carry out vacuum filtration after leaching medicinal residues, condition is: in buchner funnel, spread two layers of filter paper, filter paper therebetween 0.6cm kieselguhr, carries out vacuum filtration by coarse filtration gained filtrate, collects filtrate; Add Mel, after mixing, to obtain final product.

The preparation of embodiment 4 facial treatment milk of the present invention

Component and consumption:

Table 3

Preparation method:

(1) A heat phase is to 75 DEG C, stirs, be incubated 30 minutes for subsequent use;

(2), after B phase carbomer and deionized water dispersion hydration fully, add other raw materials of B phase, be heated to 75 DEG C and stir, be incubated 30 minutes for subsequent use;

(3) B phase homogenizing 2000 revs/min, is added in B phase by A, homogenizing 5 minutes; 30 revs/min are stirred cooling, cool to 50 DEG C, add the sodium hydroxide in C phase, stir;

(4) add the Chinese medicine extract in C phase and hyaluronate sodium, 30 revs/min, stir;

(5) 30 revs/min are stirred cooling, cool to less than 45 DEG C, add Methylisothiazolinone/iodine propilolic alcohol butyl mephenesin Carbamate, stir.

The preparation of embodiment 5 facial treatment milk of the present invention

Component and consumption:

Table 4

Preparation method:

(1) A heat phase is to 80 DEG C, stirs, be incubated 20 minutes for subsequent use;

(2), after B phase carbomer and deionized water dispersion hydration fully, add other raw materials of B phase, be heated to 85 DEG C and stir equal, be incubated 20 minutes for subsequent use;

(3) B phase homogenizing 5000 revs/min, is added in B phase by A, homogenizing 3 minutes; 50 revs/min are stirred cooling, cool to 45 DEG C, add the sodium hydroxide in C phase, stir;

(4) add Chinese medicine extract and hyaluronate sodium described in C phase, 50 revs/min, stir;

(5) 50 revs/min are stirred cooling, are cooled to less than 45 DEG C, add Methylisothiazolinone/iodine propilolic alcohol butyl mephenesin Carbamate, stir.

The preparation of embodiment 6 facial treatment milk of the present invention

Component and consumption:

Table 5

Preparation method

(1) A heat phase is to 85 DEG C, stirs, be incubated 60 minutes for subsequent use;

(2), after B phase carbomer and deionized water dispersion hydration fully, add other raw materials of B phase, be heated to 80 DEG C and stir equal, be incubated 60 minutes for subsequent use;

(3) B phase homogenizing 3000 revs/min, is added in B phase by A, homogenizing 8 minutes; 40 revs/min are stirred cooling, cool to 45 DEG C, add the sodium hydroxide in C phase, stir;

(4) add Chinese medicine extract and hyaluronate sodium described in C phase, 40 revs/min, stir;

(5) 40 revs/min are stirred cooling, are cooled to less than 45 DEG C, add Methylisothiazolinone/iodine propilolic alcohol butyl mephenesin Carbamate, stir.

Efficacy experiments of the present invention

One, embodiment 4 gained facial treatment milk A, B, C moistening effect is evaluated

1, moistening effect---human skin keratinocyte's layer hydration rate is tested

(1) test method

Test specimen is that embodiment 4 prepares gained emulsion.

Test crowd is the Chinese women of skin health, and amount to 10 people, experimenter tests first 2 days, stops using skin-protection product.

Set up experimenter's person document, and require that volunteer fills in Informed Consent Form, staff carries out instrument and experimental enviroment debugging; Temperature 22 ± 2 DEG C, relative humidity 50% ± 5%; Experimenter arrives testing location, cleaning recipient site, wipes clean, sits quietly 20 minutes;

Adopt circulation double blinding labelling;

Test starts, and this time is 0 timing, gathers subjects skin's background values, and is designated as T ₀; Sample is smeared at recipient site by technical staff, and timing; Data acquisition is carried out respectively when 1h, 2h, 4h.

For understanding in time and processing the untoward reaction that may occur after experimenter uses sample or tester, duration of test experimenter can react tested region sense of discomfort at any time, during as there is the skin irritation of obvious systemic adverse reactions or more than 3 grades, should termination test immediately, and experimenter is suitably treated.

(2) data analysis

Apparatus measures is evaluated: adopt t inspection to carry out statistical disposition to using the change in value of every measurement index before and after product, makes whether effective conclusion according to result of calculation.

(3) experimental result

Within test period, in tested crowd, do not occur the skin irritation of obvious systemic adverse reactions or more than 3 grades, therefore effective volunteer's number of this test is 10 people.

Moisture content of skin MMV:

△＝T _n-T ₀

Hydration rate=(T _n-T ₀)/T ₀, this value is higher, and moisturizing effect is more obvious.

In formula: T ₀for sample sets initial value;

Tn is sample sets each time point data acquisition set value.

Acquired results as shown in Figure 1.

As seen from Figure 1, in test period 4h, sample supplements moisture of skin successful; Inventor utilizes embodiment 5, embodiment 6 is prepared gained facial treatment milk and repeated above-mentioned experiment, and the effect that experiment finally obtains is with above-mentioned.2, skin barrier effect is repaired---moisture of skin scatters and disappears and changes

Moisture of skin windage (Transepidermalwaterloss, TEWL) reflect in test period, Experimental Area moisture loss rule over time, it can the reparation skin barrier function of characterization test sample, its value is less, moisture loss is fewer, and reparation skin barrier ability is stronger.

Moisture of skin scatters and disappears and changes the same hydration rate of test method.

Percentage of water loss (%)=(sample sets water loss amount-starting moisture windage)/starting moisture windage × 100.

Moisture loss the results are shown in Figure 2.

Result shows: in embodiment 4, gained facial treatment milk all has well reparation skin screen effect in 4h.

In sum, in embodiment 4 provided by the invention, gained facial treatment milk has skin and repairs screen effect significantly.

Inventor utilizes embodiment 5, embodiment 6 is prepared facial treatment milk and repeated above-mentioned experiment, and the effect that test finally obtains is with above-mentioned.

Two, embodiment 5 is prepared facial treatment milk and to be compacted efficacy assessments

Test specimen is that embodiment 5 prepares gained facial treatment milk.

Through the health volunteer 8 (women) that professional is trained before test, the age, all experimenters were all without skin or systemic disease history, and recipient site is without exception at 25 ~ 35 years old.

Test site room temperature (25 ± 1) DEG C, relative humidity (40 ± 5) %, before test, experimenter should keep body to be steady statue.An embodiment 5 is used to be prepared into facial treatment milk, respectively in use 5 minutes and 2 hours its effects of later evaluation.Adopt Multi-functional skin mirror image analysis system to add up result, result is as Fig. 3.Trier smears embodiment 5 and prepares 2h after gained facial treatment milk, skin compacts, moist, illustrates try out sample can the generation of stimulation collagen and repair tissue, recovered cell neogenesis power, originally be eliminated by the wrinkle lines of lax generation, skin is returned and is compacted without lines state.

Inventor utilizes that embodiment 1, embodiment 2, embodiment 3 prepare extract obtained and embodiment 4, embodiment 6 is prepared facial treatment milk and repeated above-mentioned experiment, and effect is with embodiment 5.

Three, embodiment 4 gained facial treatment milk UV resistance efficacy assessments

Suppress skin erythema test

This experiment is all through the agreement of each tester.

MED (minimal erythema dose) causes skin to produce time needed for firm visible erythema or millijoule/square centimeter (mJ/cm in fixing condition (light source and distance) ²).The mensuration of med value is the basis of photo patch test, can detect the light poison caused by photosensitive agent and photoallergy on this basis further.Assay method: random selecting is healthy without ultraviolet allergies person 30 people, before experiment, by 30 volunteers (female: 15, man: 15) upper arm is clean by clean water, irradiates at Medial upper arm with GS2006 type MULTI-WAVELENGTHRANGESPFTESTER (multi-wavelength SPF tester).The each hole 2h of the dose irradiation increased progressively by ladder, afterwards every 6h observed result.Determine everyone med value.

Experiment adopts in advance at upper arm surface smear embodiment 4 gained facial treatment milk, carries out exposing to erythema according to everyone different med value and produces afterwards, observes afterwards every 6h.

As shown in Figure 4, embodiment 4 gained facial treatment milk uvioresistant damage results shows: use facial treatment milk, 6h occurs that the number of erythema reduces 60%, 12h and occurs that the number of erythema reduces 57%; Use facial treatment milk, erythema occurs delaying the number to 18h, increases by 275%; Erythema time of origin extends to 24h number and increases 150%.Show that the present invention obviously can extend the time of erythema generation, namely postpone the generation of UV-induced skin erythema.Inventor utilizes that embodiment 1, embodiment 2, embodiment 3 prepare gained Chinese medicine extract and embodiment 5, embodiment 6 is prepared gained facial treatment milk and repeated above-mentioned experiment, and effect is with embodiment 4.

Four, embodiment 4 gained skin care milk hydraulic control oil efficacy assessments

(1) test method

Test specimen is that embodiment 4 prepares gained facial treatment milk.

Tested crowd is health volunteer 10 (women), the age at 25 ~ 35 years old, all experimenters all without skin or systemic disease history, except recipient site easy fuel-displaced except without exception.

Set up experimenter's person document, and require that volunteer fills in Informed Consent Form, staff carries out instrument and experimental enviroment debugging; Experimenter wipes after cleaning recipient site only, and the 20 ~ 30min that sits quietly;

This test employing half face test: experimental group VS matched group; Matched group half face uses distilled water as negative control; Adopt single-blind randomized principle of ordering.

Test starts, and this time is 0 timing, gathers subjects skin's background values, and is designated as T ₀.Use test sample on request, and timing; Data acquisition is carried out respectively when 1h, 2h, 4h.

For understanding in time and processing the untoward reaction that may occur after experimenter uses sample or tester, duration of test experimenter can react tested region sense of discomfort at any time, during as there is the skin irritation of obvious systemic adverse reactions or more than 3 grades, should termination test immediately, and experimenter is suitably treated.

(2) data analysis

Apparatus measures is evaluated: adopt t inspection to carry out statistical disposition to using the change in value of every measurement index before and after product, calculate the difference between each test zone initial value and other times point measured value, then this difference is utilized, the difference of statistical analysis different time points product zone.

(3) experimental result

Within test period, in tested crowd, do not occur the skin irritation of obvious systemic adverse reactions or more than 3 grades, therefore effective volunteer's number of this test is 10 people.

Before relatively not using sample, rate of change (%)=(Tn-T ₀)/T ₀× 100

The rate of change of relative blank group, i.e. relative change rate (%)=[(Tn-T ₀)-(Bn-B ₀)]/T ₀× 100

In formula, Tn---tested region changes numerical value in time

T ₀---tested region initial value

Bn---white space changes numerical value in time

B ₀---white space initial value

Experimental group and the average fat content of matched group are as shown in Figure 5.The rate of change of average fat content and relative change rate are as table 6.

Table 6

As can be seen from Fig. 5 experimental group and the average fat content of matched group and table 6 fat content rate of change and relative change rate, in test period 4h, sample sets is cashed out and is continued the oily effect of control, controls oily successful; Inventor utilizes embodiment 5, embodiment 6 is prepared gained facial treatment milk and repeated above-mentioned experiment, and the effect that experiment finally obtains is with above-mentioned.

Five, embodiment 1, embodiment 2, embodiment 3 gained Chinese medicine extract anti-electricity device Efficacy of irradiation are evaluated

(1) research purpose

By observing the impact that Chinese medicine extract A, B, C (being prepared by embodiment 1, embodiment 2 and embodiment 3 respectively) grows Mouse Skin Fibroblasts strain 3T6 after cellular radiation, evaluate Chinese medicine composition A, B, C to the cytostatic protective effect of cellular radiation.

(2) experiment material

By test product: embodiment 1, embodiment 2, embodiment 3 gained Chinese medicine extract A, B, C

Cell strain: Mouse Skin Fibroblasts strain 3T6, purchased from Chinese Academy of Sciences's cell bank, uses after this laboratory passage.

1428479), 0.25%Trypsin-EDTA (lot number: 1213981) purchased from GIBCO company reagent: FBS (lot number:.Modified form RPMI-1640 type culture medium (lot number: NXL0745) is purchased from HYCLONE company.DMSO (lot number: 20121102) purchased from Chemical Reagent Co., Ltd., Sinopharm Group.Cell proliferation-toxicity detection-CCK-8 test kit is Japanese colleague's DOJINDO chemistry institute product.

Experimental apparatus: X71 type inverted microscope is Olympus Products.HHCP-01W type carbon dioxide cell incubator is Medical Equipment Plant of Shanghai Boxun Industrial Co., Ltd. product.The single one side clean work station of SW-CJ-IFD type is Purifying Equipment Co., Ltd., Suzhou's product.RT-6000 type microplate reader, Shenzhen thunder Du Products.Nokia's mobile phone 1050 type, frequency range is GSM900MHz.

(3) experimental technique

Experiment is divided into blank group, radiation matched group, medicine variable concentrations group, often organizes 8 multiple holes.Collect logarithmic (log) phase cell, counting, by 1 × 10 ⁴/ mL concentration is inoculated into 96 orifice plates, every hole 200 μ L.Put 37 DEG C, 5%CO ₂cultivate 24 hours in cell culture incubator, make cell attachment.Medication group adds different Chinese medicine extract 100 μ L, and blank group and radiation matched group add 100 μ L serum-free mediums, continues to cultivate.After 24 hours, cell plates are placed in CO ₂incubator exoadaptation 1 hour, except blank group extracellular, other cell plates are placed on above mobile phone, and mobile phone keeps talking state, and blank group cell plates are placed in above mobile phone in another room, and mobile phone keeps closed condition.Use CCK method to detect cytoactive after 6 hours, and calculate proliferation inhibition rate and Proliferation Ability protective rate.

(4) experimental result

Result shows, use cellular radiation after 6 hours, skin flbroblast propagation is suppressed, and absorbance is reduced, radiation cellular control unit OD value comparatively blank group compares and has significant difference (P<0.01), and proliferation inhibition rate reaches 15.87%; Chinese medicine extract A, B, C dosage is at 1000 μ gmL ^-1shi Jun has significant protective effect to cell inhibitory effect after radiation, can effectively reduce radiation to fibroblastic inhibitory action, thus absorbance rises, and compares have significant difference (P<0.05) with radiation matched group.(see table 7) Chinese medicine extract A suppresses protective rate to reach 10.17% to fibroblast proliferation.

Table 7 Chinese medicine extract A, B, C are on the impact of cell proliferation/toxicity after radiation

Claims (6)

1. have moisturizing to compact the external skin-care composition of effect, it is characterized in that, described external skin-care composition is by A phase, B phase and C phase composition, and described A phase, B phase and C phase are made up of the raw material of following weight percentage ratio respectively:

Described Chinese medicine extract is made up of the crude drug of following weight parts proportioning:

Herba Dendrobii 2 ~ 10, Herba bromi japonici 1 ~ 6, Flos Caryophylli 1 ~ 3, Herba Ecliptae 2 ~ 4, the Radix Stemonae 2 ~ 5, Mel 1 ~ 5.

2. external skin-care composition as claimed in claim 1, it is characterized in that, the preparation method of described Chinese medicine extract is as follows:

(1) in crude drug described in claim 1, Herba Dendrobii, Herba bromi japonici, Flos Caryophylli, Herba Ecliptae, the Radix Stemonae take by described weight after pulverizing, mixing;

(2) extracting in water: the mass ratio of crude drug and water is 1:50 ~ 1:100,60 DEG C ~ 100 DEG C are extracted 1 ~ 4 hour to obtain extracting solution;

(3) extracting solution that step (2) obtains is cooled to 20 ~ 30 DEG C, 100 ~ 200 orders filter, and leach medicinal residues final vacuum sucking filtration, collect filtrate, add Mel, after mixing, to obtain final product.

3. external skin-care composition as claimed in claim 2, it is characterized in that, described step (1) Raw medicated powder is broken to 10 ~ 60 orders.

4. external skin-care composition as claimed in claim 2, it is characterized in that, the mass ratio of described step (2) Raw medicine and water is 1:60 ~ 1:80.

5. external skin-care composition as claimed in claim 2, it is characterized in that, in described step (3), vacuum filtration condition for spread two layers of filter paper in buchner funnel, filter paper therebetween 0.3 ~ 0.6cm kieselguhr, vacuum-0.095MPa, carries out vacuum filtration.

6. the preparation method of external skin-care composition described in any one in claim 1 to 5, it is characterized in that, described method comprises the steps:

(1) A heat phase is to 75 ~ 85 DEG C, stirs, be incubated 20 ~ 60 minutes for subsequent use;

(2) after B phase carbomer and aqueous dispersion hydration fully, add other raw materials of B phase, be heated to 75 ~ 85 DEG C and stir, be incubated 20 ~ 60min for subsequent use;

(3) B phase homogenizing 2000 ~ 5000 revs/min, is added in B phase by A, homogenizing 3 ~ 8 minutes; 30 ~ 50 revs/min are stirred cooling, cool to 45 ~ 50 DEG C, add the sodium hydroxide in C phase, stir;

(4) add Chinese medicine extract and hyaluronate sodium described in C phase, 30 ~ 50 revs/min, stir;

(5) 30 ~ 50 revs/min are stirred cooling, are cooled to less than 45 DEG C, add Methylisothiazolinone/iodine propilolic alcohol butyl mephenesin Carbamate, stir.