CN105273436A - Medlar flower pigment extraction technology - Google Patents
Medlar flower pigment extraction technology Download PDFInfo
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- CN105273436A CN105273436A CN201410341296.3A CN201410341296A CN105273436A CN 105273436 A CN105273436 A CN 105273436A CN 201410341296 A CN201410341296 A CN 201410341296A CN 105273436 A CN105273436 A CN 105273436A
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Abstract
The present invention discloses a medlar flower pigment extraction technology. The extraction method comprises the following steps: (1) medlar flowers are chopped and pulped evenly, and then mixed with hydrochloric acid, acetic acid and citric acid uniformly; (2) then solid-liquid separation is performed after homogeneous extraction; the homogeneous extraction conditions comprise operating at 5000-10 000 r/min for longer than or equal to 5min and operating at 1000-4000r / min for longer than or equal to 30min; (3) supernatant can be obtained by neutralization reaction and centrifugation of the solid-liquid separated liquid. The extraction method is rapid, low in cost, high in extraction rate and suitable for industrial production.
Description
Technical field
The invention belongs to nature strength technical field, be specifically related to a kind of extraction process of Flos Lycii pigment.
Background technology
At present, pigment used in foodstuffs industry mostly is synthetic colour, nearly all has toxicity in various degree, and the long-term edible health that can endanger people, little by little, natural pigment causes the concern of scientific research field.
Cyanidin(e) is the water-soluble natural pigment that nature one class is extensively present in plant, belong to flavonoid compound, it is as a kind of natural food colour, safe, nontoxic, aboundresources, and there is certain nutrition and pharmacological action, there is huge application potential in food, makeup, medicine and other fields.Flos Lycii is 1 ~ 2 flower that clusters, corolla is in white, lavender or purple, be rich in cyanidin(e), multiple amino acids and trace element, but in prior art, about the research report of anthocyanidin of medlar flower is little, only there is the ultrasonic extraction extracting under normal temperature condition of people's research and utilization mixing acid such as Liu Lanying, obtain pink extract, every gram of Flos Lycii extracts cyanidin(e) about 0.514 milligram of (anthocyanidin of medlar flower Study on extraction [J], Agriculture of Anhui science, 2010,38 (32): 18140-18141,18143).Ultrasonic technology is used for the extraction of natural pigment more and more by people are paid close attention to by this kind, utilizes ultrasonic mechanical effect, cavitation effect and heat effect, by increase medium molecule movement velocity, increase the penetration power of medium to extract biological effective components,
But it is high often to there is cost in its practical application, environmental noise is large, easily produces the defects such as ultrasonic white space, has certain restriction to its broadened application.
Summary of the invention
Technical problem to be solved by this invention overcomes prior art to extract anthocyanidin of medlar flower method to use ultrasonic wave to there is cost high, environmental noise is large, the defect of the ultrasonic white space of easy generation, provide a kind of quick, with low cost, extraction yield is high and be suitable for the extracting method of anthocyanidin of medlar flower of suitability for industrialized production.
The extracting method of anthocyanidin of medlar flower of the present invention comprises the steps:
(1) Flos Lycii is smash slurry evenly, then with hydrochloric acid, acetic acid and citric acid Homogeneous phase mixing;
(2) and then homogeneous extract after solid-liquid separation; Described homogeneous extraction conditions is that 5000-10000 turns/min effect >=5min, and then 1000-4000 turns/min process >=30min;
(3) liquid neutralization reaction solid-liquid separation obtained after is also centrifugal, obtains supernatant liquor.
In the present invention, described Flos Lycii is the fresh Flos Lycii that this area routine uses, and is generally the Flos Lycii petal of this area routine removing anthocaulus and pistil.The kind of described Flos Lycii is not limit.
In the present invention, described smashes slurry for the conventional said slurry of smashing in this area, generally smashes homogenate.
In the present invention, described Flos Lycii and the consumption of hydrochloric acid are preferably mass volume ratio 1: 1g/mL-1: 10g/mL, and wherein, this Flos Lycii quality is generally to smash the Flos Lycii slurry quality of starching and obtaining.
In the present invention, the concentration of described hydrochloric acid is that this area routine is used, is preferably 0.2mol/L-0.3mol/L, and that better is 0.25mol/L.
In the present invention, described acetic acid and the consumption of citric acid are this area conventional amount used, and the consumption of described acetic acid is preferably with hydrochloric acid volumeter, 5mL/100mL hydrochloric acid; The consumption of described citric acid is preferably with hydrochloric acid volumeter, 5g/100mL hydrochloric acid.
In the present invention, described homogeneous extraction conditions is first 10000 turns/min effect 10min-30min, then 3000 turns/min process 1-2 hour preferably.
In the present invention, it is that this area routine is used that described homogeneous extracts equipment used, is preferably high-shear homogenizing machine, commercially, and the SAI-3 type high-shear homogenizing machine of such as Shanghai Suo Wei.
In the present invention, described solid-liquid separation is this area routine operation, preferably for filtering.
In the present invention, after described solid-liquid separation preferably also by the filter residue of solid-liquid separation gained by step (2) repetitive operation once, merge the liquid that solid-liquid separation obtains.
In the present invention, the reagent of described neutralization reaction is that this area routine is used, is preferably sodium hydroxide.Wherein, the concentration of described sodium hydroxide is preferably 0.1mol/L.
In the present invention, described centrifugal be this area routine operation, preferably centrifugal condition is with 1000 turns/min process 5min-30min, and better is with 1000 turns/min process 10min.
The temperature of each step operation of extracting method of the present invention is that this area is conventional, is generally room temperature 0 DEG C-40 DEG C.
Preferably, the present invention also by this area routine operation, through concentrated, namely can obtain dry powder after lyophilize after obtaining supernatant liquor.
Agents useful for same of the present invention and raw material are all commercially.
On the basis meeting this area general knowledge, the optimum condition of each technical characteristic above-mentioned in the present invention arbitrary combination can obtain preferred embodiments of the present invention.
Positive progressive effect of the present invention is: the invention provides a kind of new extractive technique for anthocyanidin of medlar flower, technical support is provided for anthocyanidin of medlar flower makes full use of further, the extracting method technique of anthocyanidin of medlar flower of the present invention is simple, quick, with low cost, low to production unit requirement, extraction yield is high, obviously be better than traditional extraction method, be suitable for suitability for industrialized production.
Accompanying drawing explanation
Fig. 1 is the extracting method process flow diagram of the anthocyanidin of medlar flower of the embodiment of the present invention 1.
Fig. 2 is the ultraviolet-visible light spectrogram of the anthocyanidin of medlar flower of the embodiment of the present invention 1.
Embodiment
Mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments.
High-shear homogenizing machine is the SAI-3 type high-shear homogenizing machine of Shanghai Suo Wei.
Embodiment 1
Fig. 1 is the process flow diagram of the present embodiment.
(1) get fresh Flos Lycii, remove anthocaulus, pistil, smash slurry and tremble even, for subsequent use;
(2) homogenate of 100g is taken, add with hydrochloric acid volumeter by the quality volume fraction of solid-to-liquid ratio, Glacial acetic acid 5mL/100mL hydrochloric acid, citric acid 5g/100mL hydrochloric acid, and add the hydrochloric acid 100mL of 0.25mol/L, high speed 10000 rpms process 30 minutes under room temperature, filter after low speed 3000 rpms of lower homogeneous extract 60 minutes again, collect filter residue, filtrate respectively.It is high-shear homogenizing machine that homogeneous extracts equipment used.
(3) by filter residue by above-mentioned steps (2) reprocessing once, filter, merge twice filtrate, carry out neutralization reaction with 0.1mol/L sodium hydroxide, centrifugal 1000 turns/min process 30min after neutralization, collect supernatant liquor;
(4) supernatant liquor is through vacuum concentration, namely obtains Powdered anthocyanidin of medlar flower dry powder 60.25mg after lyophilize.
The product of the present embodiment detects through ultraviolet-visible absorption spectroscopy, as shown in Figure 2, the absorption spectrum of extract has 3 absorption peaks, respectively at 535nm, 335nm and 283nm place to result, wherein the absorption peak at 535nm place is the characteristic peak of anthocyanogen, and what show that the present embodiment extracts is anthocyanidin of medlar flower.
Embodiment 2
(1) get fresh Flos Lycii, remove anthocaulus, pistil, smash slurry and tremble even, for subsequent use;
(2) homogenate of 100g is taken, add with hydrochloric acid volumeter 5mL/100mL Glacial acetic acid, 5g/100mL citric acid by the quality volume fraction of solid-to-liquid ratio, and add the hydrochloric acid of 0.25mol/L, consumption is 500mL, high speed 10000 rpms process 20 minutes under room temperature, filter after low speed 3000 rpms of lower homogeneous extract 120 minutes again, collect filter residue, filtrate respectively.
It is high-shear homogenizing machine that homogeneous extracts equipment used.
(3) by filter residue by above-mentioned steps (2) reprocessing once, filter, merge twice filtrate, carry out neutralization reaction with 0.1mol/L sodium hydroxide, centrifugal 1000 turns/min process 5min after neutralization, collect supernatant liquor;
(4) supernatant liquor is through vacuum concentration, namely obtains Powdered anthocyanidin of medlar flower dry powder 61.08mg after lyophilize.
Embodiment 3
(1) get fresh Flos Lycii, remove anthocaulus, pistil, smash slurry and tremble even, for subsequent use;
(2) homogenate of 100g is taken, add with hydrochloric acid volumeter 5mL/100mL Glacial acetic acid, 5g/100mL citric acid by the quality volume fraction of solid-to-liquid ratio, and add the hydrochloric acid 1000mL of 0.25mol/L, high speed 10000 rpms process 10 minutes under room temperature, filter after low speed 3000 rpms of lower homogeneous extract 90 minutes again, collect filter residue, filtrate respectively.Homogeneous is carried
Getting equipment used is high-shear homogenizing machine.
(3) by filter residue by above-mentioned steps (2) reprocessing once, filter, merge twice filtrate, carry out neutralization reaction with 0.1mol/L sodium hydroxide, centrifugal 1000 turns/min process 10min after neutralization, collect supernatant liquor;
(4) supernatant liquor is through vacuum concentration, namely obtains Powdered anthocyanidin of medlar flower dry powder 60.85mg after lyophilize.
Embodiment 4
Other steps are all with embodiment 1, and wherein, described homogeneous extraction conditions is 5000 turns/min effect 5min, then 4000 turns/min process 30min; The concentration of described hydrochloric acid is 0.2mol/L.Obtain Powdered anthocyanidin of medlar flower dry powder 61.09mg.
Embodiment 5
Other steps are all with embodiment 1, and wherein, described homogeneous extraction conditions is 10000 turns/min effect 5min, then 1000 turns/min process 30min; The concentration of described hydrochloric acid is 0.3mol/L.Obtain Powdered anthocyanidin of medlar flower dry powder 61.25mg.
Claims (10)
1. a Flos Lycii Extraction of Pigment, is characterized in that: it comprises the steps:
(1) Flos Lycii is smash slurry evenly, then with hydrochloric acid, acetic acid and citric acid Homogeneous phase mixing;
(2) and then homogeneous extract after solid-liquid separation; Described homogeneous extraction conditions is that 5000-10000 turns/min effect >=5min, and then 1000-4000 turns/min process >=30min;
(3) liquid neutralization reaction solid-liquid separation obtained after is also centrifugal, obtains supernatant liquor.
2. extraction process as claimed in claim 1, is characterized in that: described Flos Lycii and the consumption of hydrochloric acid are mass volume ratio 1: 1g/mL-1: 10g/mL; Wherein, this Flos Lycii quality is to smash the Flos Lycii slurry quality of starching and obtaining.
3. extraction process as claimed in claim 1, is characterized in that: the concentration of described hydrochloric acid is 0.2mol/L-0.3mol/L, and that better is 0.25mol/L.
4. extraction process as claimed in claim 1, is characterized in that: the consumption of described acetic acid is with hydrochloric acid volumeter, 5mL/100mL hydrochloric acid; The consumption of described citric acid is with hydrochloric acid volumeter, 5g/100mL hydrochloric acid.
5. extracting method as claimed in claim 1, is characterized in that: described homogeneous extraction conditions is first 10000 turns/min effect 10min-30min, then 3000 turns/min process 1-2 hour.
6. extraction process as claimed in claim 1, is characterized in that: it is high-shear homogenizing machine that described homogeneous extracts equipment used.
7. extraction process as claimed in claim 1, is characterized in that: described solid-liquid separation is for filtering; After described solid-liquid separation also by the filter residue of solid-liquid separation gained by step (2) repetitive operation once, the liquid that solid-liquid separation obtains is merged.
8. extraction process as claimed in claim 1, is characterized in that: the reagent of described neutralization reaction is sodium hydroxide; The concentration of described sodium hydroxide is 0.1mol/L.
9. extraction process as claimed in claim 1, is characterized in that: described centrifugal condition is with 1000 turns/min process 5min-30min, is preferably with 1000 turns/min process 10min.
10. extraction process as claimed in claim 1, is characterized in that: also through concentrated after obtaining described supernatant liquor, namely obtain dry powder after lyophilize.
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| CN201410341296.3A CN105273436A (en) | 2014-07-18 | 2014-07-18 | Medlar flower pigment extraction technology |
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| CN201410341296.3A CN105273436A (en) | 2014-07-18 | 2014-07-18 | Medlar flower pigment extraction technology |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105662998A (en) * | 2016-01-29 | 2016-06-15 | 中国科学院西北高原生物研究所 | Yak hide collagen mask with ultraviolet ray resistance and preparation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105662998A (en) * | 2016-01-29 | 2016-06-15 | 中国科学院西北高原生物研究所 | Yak hide collagen mask with ultraviolet ray resistance and preparation method |
| CN105662998B (en) * | 2016-01-29 | 2018-10-16 | 中国科学院西北高原生物研究所 | A kind of Yak-skin Gelatin original facial mask and preparation method with protectiving ultraviolet |
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Application publication date: 20160127 |