CN105265486A - Bacillus marinus-pyrimethanil compounded bactericide and applications thereof - Google Patents
Bacillus marinus-pyrimethanil compounded bactericide and applications thereof Download PDFInfo
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- CN105265486A CN105265486A CN201410356151.0A CN201410356151A CN105265486A CN 105265486 A CN105265486 A CN 105265486A CN 201410356151 A CN201410356151 A CN 201410356151A CN 105265486 A CN105265486 A CN 105265486A
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Abstract
The present invention relates to Bacillus marinus-pyrimethanil compounded bactericide and applications thereof, particularly to a compounded bactericide containing Bacillus marinus and pyrimethanil, a pesticide and a plant disease prevention and control method, wherein a weight ratio of Bacillus marinus to pyrimethanil is 1:0.0005-500, and the compounded bactericide or pesticide can be especially used for preventing and controlling plant gray mold. According to the present invention, the action mechanisms of the two pesticides are different, and the two pesticides are compounded so as to provide the synergetic effect of the microorganism and the chemical agent, and achieve the purposes of advantage complementation, prevention effect improving, chemical agent consumption reducing, reduction of chemical agent residue in vegetables, fruits and flowers, and drug resistance incidence reducing.
Description
Technical field
The invention belongs to plant especially crop pest control field, be specifically related to a kind of composite bactericide and application thereof, especially a kind of by the bactericide of bacillus marinus and Pyrimethanil compositional and the application in control plant botrytis thereof.
Background technology
Gray mold is the common and a kind of fungal disease of more difficult control of open country, protecting field plant (especially crops), belongs to low temperature and high relative humidity type disease, is the disease high-incidence season when temperature 20 ~ 25 DEG C, humidity continue more than 90%.Along with the Chinese government promotes industrialized agriculture, the high humidity environment of booth impels the crops such as tomato, cucumber, and the gray mold of the flowers such as flameray gerbera, carnation is on the rise.The main means of prevention of current gray mold is chemical bactericide, but chemical bactericide excessive, frequently use, impel ash arrhizus bacteria to create pesticide resistance to chemical bactericide.Report that ash arrhizus bacteria creates pesticide resistance to various sterilization agent such as benzimidazoles both at home and abroad at present.So excessive, use chemical bactericide to prevent and treat gray mold and not only cause serious environmental pollution frequently, and directly increase the residual quantity of toxic chemical substance in vegetables, fruit, flowers, harm brought to human health.Along with growth in the living standard, the concern of people to food security is risen day by day.Microbial bactericide is because it is nuisanceless, to advantages such as person poultry safety, environmentally friendly, sick worm not easily develop immunity to drugs, day by day cause the attention of people.But field uses microbial bactericide because of by various factors, causes during actual use and also there is the problems such as such as field efficacy is unstable, field efficacy effect is slow.
The fundamental way solving this outstanding problem is: the limitation breaking through the means of prevention at present based on chemical bactericide, utilize the features such as chemical bactericide effect is quick, stable and microbial bactericide is safe, not easily develop immunity to drugs, set up efficient, the safe Disease management technical system of Biocontrol microorganism and low toxicity chemical bactericide Collaborative Control plant botrytis.By autotelic introduction beneficial microbe, improve the biological community structure using ground ecologic niche, promote beneficial microorganism population quantity, suppress the develop of the population of ash arrhizus bacteria and worsen its living environment, thus give full play to the effect of beneficial microbe biological control, significantly reduce the usage amount of chemical pesticide, realize the sustainable control of plant botrytis.
Bacillus marinus (Bacillusmarinus) is a class amphimicrobian, sporiferous Gram-positive bacillus, belongs to series bacillus and belongs to.Show that bacillus marinus can cause people, animal or plant causes a disease without any research at present, namely this bacterium is safe to people and animals, can be used for developing microbial pesticide.
The bacillus marinus B-9987 bacterial strain that East China University of Science and Zeyuan Marine life Technology Co Ltd, Shanghai obtain, from intertidal zone, Bohai Sea plant-Suaeda salsa root, has good inhibitory action to pathogenetic bacterias such as multiple disease fungus, bacterial wilt such as gray molds.This bacterial strain is identified and preservation by China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preserving number is No.2095, Classification system is BacillusmarinusB-9987 (being called for short B-9987 herein) (Luo Yuanchan etc., the qualification of marine bacteria B-9987 and the bacteriostatic test to phytopathogen thereof, " agricultural chemicals ", 47th volume the 9th phase, in September, 2008,691-693 page).Its biological character is recited as: B-9987 bacterium colony is rounded or subcircular on medium, flat, and milky is to light yellow, opaque, and Initial stage of culture bacterium colony smooth surface, neat in edge, there is gauffer on later stage surface, edge sawtooth shape.Gram-reaction is positive; Thalline is straight or closely straight shaft-like, and have a circle to oval gemma close to cell end, peritrichous, can move, aerobic growth amphimicrobian, without soluble pigment on nutrient agar; Enzyme touches reaction, glucose is positive, and nitrate reduction reaction, V-P test, indole reaction, H2S reaction, Starch Hydrolysis, hydrolysis of urea and Arabinose, L-rhamnose, fructose, wood sugar etc. utilize reaction to be feminine gender.
Bacillus marinus B-9987 also has many premium properties.As fast growth, accommodative ability of environment is strong, well surely can grow in plant rhizosphere soil, each organ of plant; Inducing plant produces disease resistance; Produce the active substance of multiple Antifungi, bacterium.Be that the microbial pesticide that biocontrol microorganisms is formulated has good preventive effect to plant botrytis with B-9987, in addition good preventive effect is also had to various bacterial diseases such as multiple fungal disease and bacterial wilt such as root rot, be that the microbial pesticide-1,000,000,000 CFU/g sea bacillus wettable powder that biocontrol microorganisms is formulated applies for registration of control of plant bacterial wilt, gray mold of cucumber at present with B-9987, the formal registration card of agricultural chemicals will be obtained the second half year in 2014.In addition, B-9987 obviously can also promote the growth of the crops such as banana, apple.
Phonetic mould amine, English common name pyrimethanil; Chemical name is N-(4,6-dimethyl pyrimidine-2-base) aniline.Former medicine is Light yellow crystals powder, fusing point 96.3 DEG C (sterling), and industrial goods content is more than 95%, most of organic solvent can be dissolved in, under room temperature, in water, solvability is 0.121g/L, and when 25 DEG C, steam pressure is 2.2mPa, stable under weak acid-weak basic condition.Determination of oil-water partition coefficient LogP is 2.84 (n-octyl alcohol/water).The former medicine of phonetic mould amine is categorized as low toxicity level by Chinese toxicity criterion.Female, the male acute oral LD50 of rat is respectively 3690mg/kg and 4300mg/kgb.w., and rat acute is greater than 2000mg/kgb.w. through skin LD50.To rabbit skin, eye irritation.Without teratogenesis, carcinogenic, mutagenesis in test dose.Phonetic mould amine mechanism of action is unique, and it can suppress pathogen Protein secretion, comprises hydrolase level, then penetrates in host tissue and makes it downright bad, thus with existing bactericide without interactivity.Phonetic mould amine can be used for the gray mold of preventing and treating the plants such as cucumber, tomato, grape, strawberry, pea, leek, flowers.
At present, having no with bacillus marinus is the report that active ingredient registers agricultural chemicals, more has no the report by bacillus marinus and Pyrimethanil compositional bactericide.
Summary of the invention
The object of the invention is to: for producing upper high frequency time, a large amount of use chemical pesticide control plant botrytis at present, cause that vegetables, fruit agriculture are residual to exceed standard and the problem of environmental pollution, propose a kind of bacillus marinus Pyrimethanil compositional bactericide and the application thereof plant botrytis to good control efficiency.After bacillus marinus B-9987 and Pyrimethanil compositional, form the effect of having complementary advantages, quick, the stable bacteriostasis of chemical agent can be played, B-9987 can be played again and after inducing plant produces disease resistance, amount reproduction, carry out site competition with pathogen and adjust the effects such as the function using microbiologic population's reasonable layout in microhabitat.By using this microorganism composite bactericide, introduce a large amount of beneficial microbe using in microhabitat, worsen the living environment of pathogen, in conjunction with agricultural measures such as cultivation managements, form stable, balance, a bio-diversity ecosystem, reach the object that drug effect increases, reduces chemical agent usage amount, reduces pesticide resistance, controls gray mold enduringly.
Therefore, first aspect present invention provides a kind of composite bactericide, and described composite bactericide contains bacillus marinus and phonetic mould amine.
In an embodiment, the weight proportion of bacillus marinus and phonetic mould amine is 1:0.0005 ~ 500.
In an embodiment, the weight proportion of bacillus marinus and phonetic mould amine is 1:0.01 ~ 300, is more preferably 1:0.05 ~ 100.
In an embodiment, the weight proportion of bacillus marinus and phonetic mould amine is 1:0.005 ~ 0.01.
In an embodiment, the weight proportion of bacillus marinus and phonetic mould amine is 1:0.05 ~ 0.1.
In an embodiment, the weight proportion of many sticky class gemma and phonetic mould amine is 1:0.2 ~ 1.
In an embodiment, the weight proportion of bacillus marinus and phonetic mould amine is 1:5 ~ 20.
In an embodiment, the weight proportion of bacillus marinus and phonetic mould amine is 1:30 ~ 50.
In an embodiment, the weight proportion of bacillus marinus and phonetic mould amine is 1:60 ~ 100.
In an embodiment, described bacillus marinus is the bacillus marinus of preserving number CGMCCNo.2095.
The present invention provides a kind of agricultural chemicals on the other hand, and described agricultural chemicals contains composite bactericide of the present invention.
In an embodiment, the formulation of described agricultural chemicals is pulvis.
In an embodiment, described agricultural chemicals is also containing acceptable carrier or auxiliary agent in Pesticide Science.
In an embodiment, described carrier be selected from White Carbon black, diatomite, starch, precipitated calcium carbonate, talcum powder, attapulgite, turfy soil, bentonite and kaolin one or more, described auxiliary agent be selected from neopelex, Tea Saponin, Tween-20, OPEO and diisooctyl Disodium sulfosuccinate one or more.
The present invention also provides a kind of method of controlling plant diseases, and described method comprises grants plant by composite bactericide of the present invention or agricultural chemicals.
In an embodiment, described plant is selected from cucumber, tomato, grape, strawberry, pea, leek and various flowers.
In an embodiment, described disease is gray mold.
The invention has the advantages that: by biocontrol microorganisms bacillus marinus B-9987 and the phonetic mould amine of chemical bactericide used in combination by a certain percentage, because bacillus marinus is completely different to the mechanism of action of pathogen with phonetic mould amine, both composite uses can not only play the phonetic mould amine bacteriostatic rate of chemical bactericide fast, to the stable advantage of gray mold control efficiency; And Biocontrol microorganism bacillus marinus B-9987 can also be kept to enclose at plant roots leaf surely grow, secrete antibacterial substance, inducing plant produces the features such as disease resistance, improves the ecotope using microhabitat.By composite, synergy, reach complementary advantage, improve the object of control efficiency; Reduce chemical agent dosage simultaneously, reduce the residual quantity of chemical agent in vegetables, fruit, flowers, make pathogen not easily produce resistance.
Embodiment
The present invention relates to containing as the bacillus marinus of active component and the composite bactericide of phonetic mould amine and agricultural chemicals.
Be applicable to bacillus marinus of the present invention and be preferably deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preserving number is No.2095, and Classification system is the bacillus marinus of BacillusmarinusB-9987 (being called for short B-9987 herein).
Such as, can use sea bacillus wettable powder disclosed in CN101331881A, this article full content is included in herein as a reference.This wetting powder contains bacillus marinus, carrier and auxiliary agent.Optional one or more in White Carbon black, diatomite, starch, precipitated calcium carbonate, talcum powder, attapulgite, turfy soil, bentonite, kaolin of carrier, auxiliary agent can be selected from neopelex, Tea Saponin, Tween-20, OPEO, diisooctyl Disodium sulfosuccinate one or more.
Usually, the active constituent content of bacillus marinus product can up to 5000-10000CFU/ μ g.After being mixed with composite bactericide of the present invention or agricultural chemicals, the content of its active ingredient can within the scope of 500-4000CFU/ μ g, such as at 1000-3500CFU/ μ g, 1000-3000CFU/ μ g, 1500-3500CFU/ μ g not etc.
Phonetic mould amine and N-(4,6-dimethyl pyrimidine-2-base) aniline, can adopt the phonetic mould amine of commercially available acquisition to prepare composite bactericide of the present invention or agricultural chemicals.Usually, commercially available phonetic mould amine is the composition containing phonetic mould amine, and wherein the content of phonetic mould amine is about 40 % by weight.Also bulk drug (i.e. the phonetic mould amine of high-purity, about about 98%) can be adopted to prepare.
Such as, simply by the bacillus marinus of aequum and phonetic mould amine mixing, after uniform stirring, just composite bactericide of the present invention and agricultural chemicals can be prepared.
In composite bactericide, the weight proportion of active ingredient bacillus marinus and phonetic mould amine is 1:0.0005 ~ 500.In a preferred embodiment, described weight proportion can be, 1:0.001 ~ 500,1:0.05 ~ 500,1:0.05 ~ 300,1:0.05 ~ 100,1,1:0.2 ~ 300,1,1:0.2 ~ 100,1:5 ~ 100,1:5 ~ 60,1:0.001 ~ 60,1:0.005 ~ 60 not etc.In some preferred embodiment, described weight proportion can be 1:0.005 ~ 0.01,1:0.05 ~ 0.1,1:0.2 ~ 1,1:5 ~ 20,1:30 ~ 50,1:60 ~ 100 not etc.
Also can containing acceptable carrier or auxiliary agent in Pesticide Science in composite bactericide and agricultural chemicals.Such as, if use the bacillus marinus product of commercially available acquisition and phonetic mould amine product, then namely other composition in these products except active ingredient bacillus marinus and phonetic mould amine can be applicable to carrier of the present invention or auxiliary agent.This kind of carrier or auxiliary agent include but not limited to previously described carrier and auxiliary agent, such as carrier is optional from White Carbon black, diatomite, starch, precipitated calcium carbonate, talcum powder, attapulgite, turfy soil, bentonite, kaolin or its any mixture, and carrier can be selected from neopelex, Tea Saponin, Tween-20, OPEO, diisooctyl Disodium sulfosuccinate or its any mixture.
The weight proportion of composite bactericide in agricultural chemicals can be such as, 0.1-99%.
Usually, in agricultural chemicals, the content of the active ingredient of bacillus marinus product can within the scope of 500-4000CFU/ μ g, such as at 1000-3500CFU/ μ g, 1000-3000CFU/ μ g, 1500-3500CFU/ μ g not etc.Phonetic mould amine can account for the 0.1-80% of agricultural chemicals weight, and such as 1-50%, 1-40%, 1-30%, 1-20%, 5-50%, 5-30% are not etc.Surplus can be acceptable carrier or auxiliary agent in Pesticide Science.
The present invention also provides a kind of method of controlling plant diseases, and described method comprises grants plant by composite bactericide of the present invention or agricultural chemicals.
" grant " and include but not limited to spray, spill.
As previously mentioned, the plant (comprising crops) being applicable to composite bactericide of the present invention or agricultural chemicals includes but not limited to cucumber, tomato, grape, strawberry, pea, leek and flowers.Described disease is gray mold.
Can be sprayed onto after composite bactericide of the present invention or agricultural chemicals dilute with water on plant (comprising crops).Consumption is generally every mu of 50-300 gram of composite bactericide of the present invention or agricultural chemicals, usually at every mu of 80-150 gram, and such as every mu about 100 grams.
Hereafter the mode with specific embodiment is described the present invention, its object is to better understand content of the present invention.Should be understood that these embodiments are only illustrative, and nonrestrictive.The reagent used in embodiment unless specifically indicated, otherwise is all that routine is buied from the market.Its usage and consumption all can use according to the usage of routine and consumption.The percentage by weight related in the present invention is the percentage by weight of pure material.
Embodiment 1: the control bacillus marinus of gray mold of cucumber and the composition of phonetic mould amine, its weight proportion is bacillus marinus: phonetic mould amine=1:0.005 ~ 0.01.The present invention is according to 5000CFU/ μ g bacillus marinus, and concentration is 40% phonetic mould amine batching.All the other embodiments are also identical.
Following table 1 shows concrete medicament, consumption and control efficiency.
Table 1: control gray mold of cucumber pharmacy effect
(data source was in field control effectiveness test in 2012)
Embodiment 2: the control bacillus marinus of gray mold of cucumber and the composition of phonetic mould amine, its weight proportion is bacillus marinus: phonetic mould amine=1:0.05 ~ 0.1.
Following table 2 shows concrete medicament, consumption and control efficiency.
Table 2: control gray mold of cucumber pharmacy effect compares
(data source was in field control effectiveness test in 2012)
Embodiment 3: the control bacillus marinus of gray mold of cucumber and the composition of phonetic mould amine, its weight proportion is bacillus marinus: phonetic mould amine=1:0.2 ~ 1.
Following table 3 shows concrete medicament, consumption and control efficiency.
Table 3: control gray mold of cucumber pharmacy effect
(data source was in field control effectiveness test in 2012)
Embodiment 4: the control bacillus marinus of gray mold of cucumber and the composition of phonetic mould amine, its weight proportion is bacillus marinus: phonetic mould amine=1:5 ~ 20.
Following table 4 shows concrete medicament, consumption and control efficiency.
Table 4: control gray mold of cucumber pharmacy effect
(data source was in field control effectiveness test in 2012)
Embodiment 5: the control bacillus marinus of gray mold of cucumber and the composition of phonetic mould amine, its weight proportion is bacillus marinus: phonetic mould amine=1:30 ~ 50.
Following table 5 shows concrete medicament, consumption and control efficiency.
Table 5: control gray mold of cucumber pharmacy effect
(data source was in field control effectiveness test in 2012)
Embodiment 6: the control bacillus marinus of gray mold of cucumber and the composition of phonetic mould amine, its weight proportion is bacillus marinus: phonetic mould amine=1:60 ~ 100.
Following table 6 shows concrete medicament, consumption and control efficiency.
Table 6: control gray mold of cucumber pharmacy effect
(data source was in field control effectiveness test in 2012)
Embodiment 7: composite bactericide is mixed with powder-type agricultural chemicals, in composite bactericide, active component content is: bacillus marinus 3000CFU/ μ g, and phonetic mould amine content is 12% (with agricultural chemicals weighing scale).
Following table 6 shows concrete medicament, consumption and control efficiency.
Table 7: control gray mold of cucumber pharmacy effect
(data source was in field control effectiveness test in 2012)
Embodiment 8: the formulations of pesticide are the composite bactericide of the control plant botrytis of pulvis, and in composite bactericide, active component content is: bacillus marinus 3000CFU/ μ g, phonetic mould amine content is 12% (with agricultural chemicals weighing scale).
Following table 8 shows concrete medicament, consumption and control efficiency.
Table 8: control graw mold of tomato pharmacy effect
(data source was in field control effectiveness test in 2012)
Embodiment 9: the formulations of pesticide are the composite bactericide of the control plant botrytis of pulvis, and in composite bactericide, active component content is: bacillus marinus 3000CFU/ μ g, phonetic mould amine content is 12% (with agricultural chemicals weighing scale).
Following table 9 shows concrete medicament, consumption and control efficiency.
Table 9: control grape grey mould pharmacy effect
(data source was in field control effectiveness test in 2012)
Embodiment 10
Bacillus marinus B-9987 bacterial strain has natural resistance to phonetic mould amine, through PB solid culture medium (the sucrose 10g that induction can be 1000ppm at phonetic mould amine content, Yeast diffusion juice 4.5g, sodium chloride 0.5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.5g, agar 15g, water 1000ml) upper normal growth.
Table 10: the many sticky growing state of class gemma bar on the phonetic mould amine PB flat board of different content
In table 10, lowercase and capitalization are illustrated respectively in the significance of difference of 0.05 and 0.01 level, and alphabetical identical table differential is different not remarkable, and letter is different represents significant difference.
Claims (10)
1. a composite bactericide, is characterized in that, described composite bactericide contains bacillus marinus and phonetic mould amine.
2. composite bactericide as claimed in claim 1, it is characterized in that, the weight proportion of described bacillus marinus and phonetic mould amine is 1:0.0005 ~ 500, is preferably 1:0.01 ~ 300, is more preferably 1:0.05 ~ 100.
3. composite bactericide as claimed in claim 2, it is characterized in that, the weight proportion of described bacillus marinus and phonetic mould amine is 1:0.05 ~ 0.1,1:0.2 ~ 1,1:5 ~ 20,1:30 ~ 50 or 1:60 ~ 100.
4. the composite bactericide according to any one of claim 1-3, is characterized in that, described bacillus marinus is the bacillus marinus of preserving number CGMCCNo.2095.
5. an agricultural chemicals, is characterized in that, described agricultural chemicals contains the composite bactericide according to any one of claim 1-5.
6. agricultural chemicals as claimed in claim 5, it is characterized in that, the described formulations of pesticide are pulvis.
7. the agricultural chemicals according to any one of claim 5-6, is characterized in that, described agricultural chemicals is also containing acceptable carrier or auxiliary agent in Pesticide Science.
8. a method for controlling plant diseases, is characterized in that: described method comprises grants plant by the composite bactericide according to any one of claim 1-4 or the agricultural chemicals according to any one of claim 5-7.
9. method as claimed in claim 8, it is characterized in that, described plant is selected from cucumber, tomato, grape, strawberry, pea, leek and flowers.
10. method as claimed in claim 8 or 9, it is characterized in that, described disease is gray mold.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101331881A (en) * | 2007-06-27 | 2008-12-31 | 国家海洋局第一海洋研究所 | Sea bacillus wettable powder, and preparation method and use thereof |
| CN101700041A (en) * | 2009-11-20 | 2010-05-05 | 江苏省农业科学院 | Bacillus subtilis and pyrimethanil compounded bactericide and application thereof |
| CN102845475A (en) * | 2012-09-11 | 2013-01-02 | 倪烈 | Compound pesticide used for controlling paddy rice sheath blight |
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- 2014-07-24 CN CN201410356151.0A patent/CN105265486A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101331881A (en) * | 2007-06-27 | 2008-12-31 | 国家海洋局第一海洋研究所 | Sea bacillus wettable powder, and preparation method and use thereof |
| CN101700041A (en) * | 2009-11-20 | 2010-05-05 | 江苏省农业科学院 | Bacillus subtilis and pyrimethanil compounded bactericide and application thereof |
| CN102845475A (en) * | 2012-09-11 | 2013-01-02 | 倪烈 | Compound pesticide used for controlling paddy rice sheath blight |
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